CN103518509B - A kind of Wand Cutting Techniques of Cyclocarya paliurus method - Google Patents
A kind of Wand Cutting Techniques of Cyclocarya paliurus method Download PDFInfo
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- CN103518509B CN103518509B CN201310472020.4A CN201310472020A CN103518509B CN 103518509 B CN103518509 B CN 103518509B CN 201310472020 A CN201310472020 A CN 201310472020A CN 103518509 B CN103518509 B CN 103518509B
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Abstract
A kind of Wand Cutting Techniques of Cyclocarya paliurus method; mainly comprise the process of cuttings elite stand, cutting medium configuration and process, growth hormone process, cuttings is pruned and cuttage, slotting to manage afterwards, the multinomial step such as transplanting; utilize new matrix, hormone prescription cuttage and seedling culture new method; solve blue or green money willow cuttage callus and form the problems such as difficulty, Organ Differentiation is difficult, rootage duration is long, rooting rate is low; be a kind of means that a kind of protective development utilizes blue or green money willow plant resources, also can be Germ-plasma resources protection recycling and strong technical guarantee is provided.
Description
Technical field
The present invention relates to the vegetative propagation technique of plant, be specially a kind of Wand Cutting Techniques of Cyclocarya paliurus method.
Background technology
According to interrelated data, blue or green money willow is the rare tree species that glacier four discipline survives, and belongs to national second class protection seeds, and be the distinctive natural health care resource of China, at south China, many provinces are all found, mainly with being sporadicly distributed as master.It is generally acknowledged, containing the abundant several kinds of mineral elements relevant with health and hypoglycemic activity in blue or green money willow, its root, branch, Ye Junke are used as medicine, simultaneously, among the peoplely make the health tea also history of existing about 200 years with its blade, this health protection tea tea gas delicate fragrance, taste is sweet in Radix Glycyrrhizae, therefore has the title of " sweet tea tree ".Generally speaking, blue or green money willow be a kind ofly integrate economy material, greening is viewed and admired, tea-drinking health care, the multiple unique function such as medicinal treatment health food resource, value of exploiting and utilizing has a extensive future.
But, due to the conventional seminal propagation of blue or green money willow, germination rate is quite low, and growth differs, even plant tissue culture is also just started in recent years to involve in starting stage, not break-through skill bottleneck, the long rooting rate of cottage propagation rootage duration is not high, and reproduction coefficient is little, breeding scale is limited, causes blue or green money willow seedling in the market to hold at high price.Because nursery stock quantity is few, be difficult to meet the demands such as growing tea-drinking health care and medical applications, seriously constrain the industrialization development of blue or green money willow.
And from first section of pertinent literature report in 2005 so far, the subject matter that blue or green money willow exists in group training cultivation is: callus is difficult to occur Organ Differentiation after subculture and easy brownization, inducing clumping bud are difficult, though also there are some documents to instruct plantation, but repetitive rate is low, be difficult to realize extensive nursery.And relative to tissue culture technique, the seedling raise period of taking root of cottage propagation wants shorter, but by literature search, utilize Technique on Cuttage Propagation to breed, its rooting rate is the highest to be also just between 70% ~ 80%, and rootage duration is more than 60 days, thus, also there is larger limitation.Therefore, carry out blue or green money willow cuttage and seedling culture new industrial research, breed a large amount of nursery stocks in a short time, to meet the demand in market, be significant.
Summary of the invention
Technical problem solved by the invention is to provide a kind of Wand Cutting Techniques of Cyclocarya paliurus method, this method utilizes new matrix and hormone prescription to carry out cuttage and seedling culture, can form the problems such as difficulty, Organ Differentiation is difficult, rootage duration is long, rooting rate is low in order to solve blue or green money willow cuttage callus.
Technical problem solved by the invention realizes by the following technical solutions:
A kind of Wand Cutting Techniques of Cyclocarya paliurus method, specifically comprises following operating procedure:
1, elite stand process: in order to obtain excellent germinating spray, in 1 ~ February then by the process of scion-seed tree cutting strunk, cutting strunk height 15cm ~ 20cm.
2, cutting medium process: be that peat soil and the perlite of 1 ~ 2mm is mixed and made into cutting medium according to the volume ratio of 2:1 by particle diameter, and the cutting medium configured is loaded the seedling culture hole plate of 50 cave specifications, cave mouth diameter is 6cm, the degree of depth is 11cm.
3, growth hormone process: the germinating branch that clip is disease-free, select semi-lignified degree cuttings, each cuttings is about 8 ~ 10cm, and have and comprise 2 axillalry buds, and retain 2 half leaves, with the tpn solution of concentration 1000 times, cuttings is sterilized, then the base portion of cuttings is stained with mud dipping, soak time 60min in 600ppmIBA growth hormone solution.
4, cuttage: first matrix is inserted a duck eye during cuttage, then inserted in duck eye by the germinating branch handled well, the degree of depth, at about 5cm, suitably compresses.Then to drench clear water, spray the tpn solution of 1000 times 75%.
5, management after inserting: be placed in green house by the seedling culture hole plate of good for cuttage nursery stock, every 10min atomized water spray 10s, makes that environmental temperature remains on 25 DEG C ~ 32 DEG C, humidity remains on more than 95%.
Under management condition of the present invention, the spray that cuttage is good starts to take root for about 20 days, within about 30 days, can form complete root system.According to 30 days investigation statisticses, on average take root several more than 5, rooting rate reached 92%, because being container nursery, need not hardening, and can select as required to transplant, afforestation of going up a hill.
In the present invention, to scion-seed tree adopt that fringe cutting time selects annual early June, mid-August for best, and because blue or green money willow enters resting stage after entering November, no longer adopts fringe cuttage and seedling culture.
In the present invention, must with tpn or the process of thiophanate methyl solution disinfection before described cuttings cuttage.
Advantage of the present invention and beneficial effect are:
1, use the formation time of the root restriction of the nursery stock of cuttage and seedling culture method of the present invention effectively to shorten, about 30d can form complete root system, and rootage duration is relative within 2 months, shortening one times needed for traditional cuttage and seedling culture method;
2, use the nursery stock cuttage root-taking number of cuttage and seedling culture method of the present invention to be generally more than five, object and the effect of improved seeds fast breeding can be reached sooner;
3, use the cutting plantation of the nursery stock of cuttage and seedling culture method of the present invention usually more than 92%, relative to 70% ~ 80% of nursery stock cutting plantation in prior art, exceed at least ten two percentage points.
Embodiment
Give an actual example below and describe the present invention.
Embodiment one:
Wand Cutting Techniques of Cyclocarya paliurus is carried out in certain experimental forest nursery northern, Hunan, first, in January then by the process of scion-seed tree cutting strunk, cutting strunk height 15cm; Then in seedling culture hole plate, carry out cutting medium process, in the present embodiment, the peat soil of cutting medium to be particle diameter be 1 ~ 2mm mixes soil property with perlite in the ratio of 2:1 is mixed, and its cave mouth diameter is 6cm, the degree of depth is 11cm; The germinating branch that clip is disease-free, select semi-lignified degree cuttings, and keep each cutting length between 8 ~ 10cm, and have and comprise 2 axillalry buds, and retain 2 half leaves, with the tpn solution of concentration 1000 times, cuttings is sterilized, then the base portion of cuttings is stained with mud dipping, soak time 60min in 600ppmIBA growth hormone solution; Then carry out a cuttage respectively in early June and mid-August, its cuttage amount is 1:1, first matrix is inserted a duck eye during cuttage, and then inserted in duck eye by the germinating branch handled well, the degree of depth, at about 5cm, suitably compresses.Then drench clear water, the tpn solution spraying 1000 times 75% carries out sterilization treatment, and then just the seedling culture hole plate of the good nursery stock of cuttage is placed in green house, and every 10min atomized water spray 10s, make environmental temperature remain on about 25 DEG C, humidity remains on more than 95%.
In the present embodiment, the seedling after cuttage started at 20 days to take root, within 30 days, form complete root system, and rootage duration is about 25 days, and on average take root several more than 5, cutting plantation reaches 92.3%, and transplanting survival rate is 96.4%.
Embodiment two:
Wand Cutting Techniques of Cyclocarya paliurus is carried out in Suining County of Hunan Province ecological agricultural garden, first, in January then by the process of scion-seed tree cutting strunk, cutting strunk height 20cm; Then in seedling culture hole plate, carry out cutting medium process, in the present embodiment, the peat soil of cutting medium to be particle diameter be 1 ~ 2mm mixes soil property with perlite in the ratio of 2:1 is mixed, and its cave mouth diameter is 6cm, the degree of depth is 11cm; The germinating branch that clip is disease-free, select semi-lignified degree cuttings, and keep each cutting length between 8 ~ 10cm, and have and comprise 2 axillalry buds, and retain 2 half leaves, with the tpn solution of concentration 1000 times, cuttings is sterilized, then the base portion of cuttings is stained with mud dipping, soak time 60min in 600ppmIBA growth hormone solution; Then carry out a cuttage respectively in early June and mid-August, its cuttage amount is 1:1, first matrix is inserted a duck eye during cuttage, and then inserted in duck eye by the germinating branch handled well, the degree of depth, at about 5cm, suitably compresses.Then drench clear water, the tpn solution spraying 1000 times 75% carries out sterilization treatment, and then just the seedling culture hole plate of the good nursery stock of cuttage is placed in green house, and every 10min atomized water spray 10s, make environmental temperature remain on about 28 DEG C, humidity remains on more than 95%.
In the present embodiment, the seedling after cuttage started at the 20th day to take root, within 30 days, form complete root system, and rootage duration is about 25 days, and on average take root several more than 5, cutting plantation reaches 92.8%, and transplanting survival rate is 96.2%.
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (6)
1. a Wand Cutting Techniques of Cyclocarya paliurus method, is characterized in that, specifically comprises following operating procedure:
1. elite stand process: in 1 ~ February then by the process of scion-seed tree cutting strunk;
2. cutting medium process: peat soil and perlite particle are mixed and made into cutting medium, and the cutting medium configured is loaded seedling culture hole plate;
3. growth hormone process: the germinating branch that clip is disease-free from the elite stand after 1. step processes, select semi-lignified degree cuttings, long 8 ~ the 10cm of each cuttings, and have and comprise 2 axillalry buds, and retain 2 half leaves, with the tpn solution of 1000 times of concentration, cuttings is sterilized, then the base portion of cuttings is stained with mud dipping in 600ppmIBA growth hormone solution;
4. cuttage: during cuttage, the germinating branch handled well is inserted in the matrix of described seedling culture hole plate, the degree of depth is at about 5cm, and compress, clear water of then drenching, sprays the tpn solution of 1000 times 75%;
Management after inserting: be placed in green house by the seedling culture hole plate of good for cuttage nursery stock and cultivate, every 10min atomized water spray 10s, makes during cultivation that environmental temperature remains on 25 DEG C ~ 32 DEG C, humidity remains on more than 95%.
2. a kind of Wand Cutting Techniques of Cyclocarya paliurus method according to claim 1, is characterized in that, selects annual early June or mid-August to the fringe cutting time of adopting of scion-seed tree.
3. a kind of Wand Cutting Techniques of Cyclocarya paliurus method according to claim 1, is characterized in that, step 1. in the cutting strunk height of cutting strunk process of elite stand process be 15cm ~ 20cm.
4. a kind of Wand Cutting Techniques of Cyclocarya paliurus method according to claim 1, is characterized in that, step 2. middle peat soil and perlitic particle diameter is 1 ~ 2mm, and its mixed proportion is the volume ratio of 2:1.
5. a kind of Wand Cutting Techniques of Cyclocarya paliurus method according to claim 1, is characterized in that, the cave mouth diameter of the nursery cave basin that step is 2. adopted is 6cm, the degree of depth is 11cm.
6. a kind of Wand Cutting Techniques of Cyclocarya paliurus method according to claim 1, is characterized in that, step 3. in the base portion of the cuttings soak time of being stained with mud dipping in growth hormone solution be 60min.
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| CN103947410A (en) * | 2014-04-21 | 2014-07-30 | 北京绿源智林农业研究院 | Rapid Salix-chaenomeloides-twig cutting-propagation technology |
| CN104798643A (en) * | 2015-03-26 | 2015-07-29 | 安徽华宇工艺品集团有限公司 | High-yield planting method of green-branch willow |
| CN105010136B (en) * | 2015-06-30 | 2017-03-22 | 略阳县锦绣农业发展有限公司 | Cyclocarya paliurus non-tube rapid propagation method |
| CN104904489B (en) * | 2015-07-08 | 2018-03-20 | 四川农业大学 | High Pentaclethra macrophylla low altitude area propagation method |
| CN105052458A (en) * | 2015-07-28 | 2015-11-18 | 龙胜泓文生态农林科技贸易有限公司 | Planting method of zinc-supplemented cyclocarya paliurus |
| CN105061028A (en) * | 2015-07-28 | 2015-11-18 | 龙胜泓文生态农林科技贸易有限公司 | Matrix for cutting propagation of cyclocarya paliurus and processing method thereof |
| CN105052609A (en) * | 2015-07-28 | 2015-11-18 | 龙胜泓文生态农林科技贸易有限公司 | Matrix for cutting and breeding of cyclocarya paliurus and preparation method thereof |
| CN104982253A (en) * | 2015-07-28 | 2015-10-21 | 龙胜泓文生态农林科技贸易有限公司 | Cyclocarya paliurus cutting propagation substrate and processing method thereof |
| CN105027926A (en) * | 2015-07-28 | 2015-11-11 | 龙胜泓文生态农林科技贸易有限公司 | Planting method of cyclocarya paliurus of supplementing selenium |
| CN105123402A (en) * | 2015-08-28 | 2015-12-09 | 龙胜泓文生态农林科技贸易有限公司 | Method for preventing and treating cyclocarya paliurus insect attack |
| CN106538161A (en) * | 2015-09-16 | 2017-03-29 | 贵州琦福苑茶业有限公司 | A kind of cuttage and seedling culture method of high-perfume type black tea new varieties gold Paeonia suffruticosa |
| CN106105983A (en) * | 2016-06-30 | 2016-11-16 | 南京林业大学 | A kind of Cyclocarya paliurus Iljinskaja Container nursery technique of optimization |
| CN106305276A (en) * | 2016-08-19 | 2017-01-11 | 武冈市青钱柳茶业有限公司 | Cyclocarya paliurus root cutting propagation method |
| CN109348883A (en) * | 2018-12-07 | 2019-02-19 | 怀化职业技术学院 | Improve the method for culturing seedlings of juglandaceae plant cuttage seeding transplanting survival rate |
| CN110881322A (en) * | 2019-12-19 | 2020-03-17 | 绥宁县神农金康药用植物科技开发有限公司 | Cultivation method capable of quickly cultivating cyclocarya paliurus tea |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138416A (en) * | 2011-01-13 | 2011-08-03 | 南京林业大学 | Cuttage propagation method for twig of cyclocarya paliurus |
| CN102487679A (en) * | 2011-11-17 | 2012-06-13 | 赵声驰 | Cutting propagation method for lithocarpus polystachyus rehd |
| CN102860204A (en) * | 2012-09-18 | 2013-01-09 | 王继华 | Cuttage propagation method for styrax tonkinensis |
-
2013
- 2013-10-11 CN CN201310472020.4A patent/CN103518509B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138416A (en) * | 2011-01-13 | 2011-08-03 | 南京林业大学 | Cuttage propagation method for twig of cyclocarya paliurus |
| CN102487679A (en) * | 2011-11-17 | 2012-06-13 | 赵声驰 | Cutting propagation method for lithocarpus polystachyus rehd |
| CN102860204A (en) * | 2012-09-18 | 2013-01-09 | 王继华 | Cuttage propagation method for styrax tonkinensis |
Non-Patent Citations (4)
| Title |
|---|
| 波叶红果树扦插繁殖技术研究;蒋挺等;《中国观赏园艺研究进展》;20090818;第272-276页 * |
| 神茶原料青钱柳扦插繁殖试验初报;夏小华等;《蚕桑茶叶通讯》;19941231(第2期);第12-14页 * |
| 青钱柳嫩枝扦插影响因素的研究;谢风;《安徽农业科学》;20091231;第37卷(第9期);第4014-4015页 * |
| 青钱柳嫩枝扦插育苗的研究;郭春兰等;《江西农业大学学报》;20060430;第28卷(第2期);第254-257页 * |
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