CN103518509A - Shoot cutting seedling-culturing method of cyclocarya paliurus - Google Patents

Abstract

A method for growing seedlings of Cyclocarya twigs by cuttings, which mainly includes multiple steps such as treatment of cutting mother trees, configuration and treatment of cutting substrates, auxin treatment, pruning of cuttings and cuttings, management after cuttings, transplanting, etc., using new substrates and hormone formula for cuttings The new method of raising seedlings solves the problems of difficult tissue formation, organ differentiation, long rooting time, and low rooting rate of Cyclocarya paliurus cuttings. Provide strong technical support for the preservation and reuse of high-quality resources.

Description

A method for growing seedlings of Cyclocarya twigs by cuttings

technical field

The invention relates to the asexual reproduction technology of plants, in particular to a method for growing seedlings by cuttings of Cyclocarya paliurus twigs.

Background technique

According to relevant information, Cyclocarya paliurus is a rare tree species that survived the Quaternary Glacier and belongs to the national second-class protected tree species. It is a unique natural health food resource in my country. It is found in many provinces in southern my country, mostly in sporadic distribution. It is generally believed that Cyclocarya paliurus is rich in various mineral elements related to human health and hypoglycemic effect, and its roots, branches, and leaves can be used as medicine. With a history of more than 100 years, this health-care tea has a delicate fragrance and taste as sweet as licorice, so it is called "sweet tea tree". All in all, Cyclocarya paliurus is a health food resource that integrates multiple unique functions such as economical materials, greening and ornamental, tea health care, medicinal treatment, etc., and has broad prospects for development and utilization.

However, due to the conventional seed propagation of Cyclocarya paliurus, the germination rate is quite low and the growth varies. Plant tissue culture has only been involved in the initial stage in recent years and has not broken through the technical bottleneck. The rooting time of cutting propagation is long and the rooting rate is not high. , the reproduction coefficient is small, and the reproduction scale is limited, resulting in the current high price of Cyclocarya paliurus seedlings in the market. Due to the small number of seedlings, it is difficult to meet the growing demand for tea health care and medical applications, which seriously restricts the industrial development of Cyclocarya paliurus.

However, since the first related literature report in 2005, the main problems of Cyclocarya paliurus in tissue culture are: it is difficult for the callus to undergo organ differentiation after subculture, it is easy to brown, and it is difficult to induce clustered buds. Some literature guides planting, but the repetition rate is low, and it is difficult to achieve large-scale seedling cultivation. Compared with tissue culture technology, the rooting time of cutting propagation is shorter. However, according to literature search, the highest rooting rate of cutting propagation technology is only between 70% and 80%, and the rooting time is more than 60 days. , therefore, has a relatively large limitation. Therefore, it is of great significance to carry out the research on the new technology of cutting seedlings of Cyclocarya paliurus, and breed a large number of seedlings in a short period of time to meet the needs of the market.

Contents of the invention

The technical problem to be solved by the present invention is to provide a method for growing seedlings by cuttings of Cyclocarya twigs. This method utilizes new substrates and hormone formulas for cutting seedlings, which can be used to solve the problem of difficult tissue formation and organ differentiation of Cyclocarya carya cuttings. , Long rooting time, low rooting rate and other problems.

The technical problem solved by the present invention adopts following technical scheme to realize:

A method for growing seedlings of Cyclocarya twigs by cuttings, specifically comprising the following steps:

1. Mother tree treatment: In order to obtain excellent sprouting shoots, the ear-picking mother tree is cut off in January to February of that year, and the cut height is 15cm to 20cm.

2. Cutting substrate treatment: Mix peat soil with a particle size of 1~2mm and perlite at a volume ratio of 2:1 to make a cutting substrate, and put the prepared cutting substrate into a seedling tray with a size of 50 holes. The diameter is 6cm and the depth is 11cm.

3. Auxin treatment: cut out the germinated branches without disease, select the semi-lignified cuttings, each cutting is about 8-10cm long, and contains 2 axillary buds, and keep 2 and a half leaves. The cuttings were sterilized by the bacteria clear solution, and then the base of the cuttings was soaked in mud in 600ppm IBA auxin solution for 60 minutes.

4. Cutting: When cutting, first insert the substrate into a small hole, and then insert the treated sprouted branches into the small hole to a depth of about 5cm, and press it properly. Then drenched with clear water and sprayed 1000 times of 75% chlorothalonil solution.

5. Post-insertion management: Place the seedling trays of the cut seedlings in the greenhouse, and spray water mist for 10 seconds every 10 minutes to keep the ambient temperature at 25°C~32°C and the humidity above 95%.

Under the management conditions of the present invention, the cuttages start to take root in about 20 days, and can form a complete root system in about 30 days. According to the 30-day survey and statistics, the average number of roots is more than 5, and the rooting rate reaches 92%. Because the seedlings are raised in containers, there is no need to harden the seedlings. You can choose to transplant or go up the mountain for afforestation according to your needs.

In the present invention, it is best to choose early June and mid-August every year for the cutting time of the mother tree for picking ears, and after entering November, because Cyclocarya paliurus enters the dormant period, no cuttings are used to grow seedlings.

In the present invention, the cuttings must be sterilized with chlorothalonil or thiophanate-methyl solution before cutting.

Advantage of the present invention and beneficial effect are:

1. The formation time of the root primordium of the seedlings using the cutting seedling raising method of the present invention can be effectively shortened, and a complete root system can be formed in about 30 days, and the rooting time is doubled compared to the 2 months required by the traditional cutting seedling raising method ;

2. The number of rooted seedling cuttings using the method for growing seedlings by cuttings of the present invention is generally more than five, which can achieve the purpose and effect of rapid breeding of fine varieties more quickly;

3. The cutting rooting rate of seedlings using the cutting seedling raising method of the present invention is usually above 92%, which is at least 12 percentage points higher than the 70% to 80% of the cutting rooting rate of seedlings in the prior art.

Detailed ways

The present invention is described in detail with examples below.

Embodiment one:

In a nursery of an experimental forest farm in the north of Hunan, the cuttings of Cyclocarya paliurus twigs were used to raise seedlings. First, in January of that year, the mother trees were cut to a height of 15 cm; In the example, the cutting substrate is a mixture of peat soil with a particle size of 1~2mm and perlite at a ratio of 2:1. The hole diameter is 6cm and the depth is 11cm; Cuttings with a degree of lignification, and keep the length of each cutting between 8 and 10 cm, and contain 2 axillary buds, and keep 2 half leaves, sterilize the cuttings with a 1000-fold concentration of chlorothalonil solution, and then sterilize the base of the cuttings Dip in mud and soak in 600ppm IBA auxin solution for 60 minutes; then carry out cuttings in early June and mid-August, and the cutting amount is 1:1. Insert the germinated branches into the small hole, the depth is about 5cm, and press it properly. Then drench clear water, spray 1000 times 75% chlorothalonil solution for sterilization, then place the seedling trays with cuttages in the greenhouse, spray water mist for 10 seconds every 10 minutes, and keep the ambient temperature at 25°C The humidity is kept above 95%.

In this example, the seedlings after cutting began to take root in 20 days, and formed a complete root system in 30 days. The rooting time was about 25 days, the average number of roots was more than 5, the cutting rooting rate reached 92.3%, and the transplanting survival rate was 96.4% %.

Embodiment two:

In an ecological agricultural garden in Suining County, Hunan Province, the cuttings of Cyclocarya paliurus twigs were raised. First, in January of that year, the mother tree was cut off to a height of 20 cm; In this embodiment, the cutting substrate is the mixed soil quality after peat soil and perlite with a particle size of 1-2 mm are mixed in a ratio of 2:1, and the diameter of the hole mouth is 6 cm and the depth is 11 cm; Select semi-lignified cuttings, keep the length of each cutting between 8 and 10 cm, and contain 2 axillary buds, and keep 2 and a half leaves, sterilize the cuttings with a 1000-fold concentration of chlorothalonil solution, and then sterilize the cuttings Soak the base in 600ppm IBA auxin solution with mud for 60 minutes; then cuttages were carried out in early June and mid-August, and the cutting amount was 1:1. When cutting, first insert a small hole in the matrix, and then put Insert the treated sprouted branches into the small hole with a depth of about 5cm, and press them properly. Then drench clear water, spray 1000 times 75% chlorothalonil solution for sterilization, and then place the seedling trays with cuttages in the greenhouse, spray water mist for 10 seconds every 10 minutes, and keep the ambient temperature at 28°C The humidity is kept above 95%.

In this example, the seedlings after cutting began to take root on the 20th day, and formed a complete root system on the 30th day. The rooting time was about 25 days, the average number of roots was more than 5, the cutting rooting rate reached 92.8%, and the transplanting survival rate 96.2%.

The basic principles and main features of the present invention and the advantages of the present invention have been shown and described above. Those skilled in the industry should understand that the present invention is not limited by the above-mentioned embodiments. What are described in the above-mentioned embodiments and the description only illustrate the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will also have Variations and improvements are possible, which fall within the scope of the claimed invention. The protection scope of the present invention is defined by the appended claims and their equivalents.

Claims (6)

1. a Wand Cutting Techniques of Cyclocarya paliurus method, is characterized in that, specifically comprises following operating procedure:

1. elite stand is processed: 1 ~ February scion-seed tree cutting strunk is being processed then;

2. cutting medium is processed: peat soil and perlite particle are mixed and made into cutting medium, and pack the cutting medium configuring into seedling culture hole plate;

3. growth hormone is processed: the disease-free germinating branch of clip on the elite stand from 1. step is processed, select semi-lignified degree cuttings, each cuttings is about 8～10cm, and have and comprise 2 axillalry buds, and retain 2 half sheet leaves, with the tpn solution of 1000 times of concentration, cuttings is sterilized, then the base portion of cuttings is stained with to mud dipping in 600ppmIBA growth hormone solution;

4. cuttage: during cuttage, the germinating branch of handling well is inserted in the matrix of described seedling culture hole plate, the degree of depth, in 5cm left and right, compresses, and the clear water of then drenching, sprays the tpn solution of 1000 times 75%;

Management after inserting: the seedling culture hole plate of the good nursery stock of cuttage is placed in green house and is cultivated, and every 10min atomized water spray 10s, makes environmental temperature remain on 25 ℃ ~ 32 ℃, humidity and remain on more than 95% during cultivation.

2. a kind of Wand Cutting Techniques of Cyclocarya paliurus method according to claim 1, is characterized in that, the fringe cutting time of adopting of scion-seed tree is selected to annual early June or mid-August.

3. a kind of Wand Cutting Techniques of Cyclocarya paliurus method according to claim 1, is characterized in that, step 1. in the cutting strunk height processed of the cutting strunk processed of elite stand be 15cm ~ 20cm.

4. a kind of Wand Cutting Techniques of Cyclocarya paliurus method according to claim 1, is characterized in that, step 2. in peat soil and perlitic particle diameter be 1 ~ 2mm, the volume ratio that its mixed proportion is 2:1.

5. a kind of Wand Cutting Techniques of Cyclocarya paliurus method according to claim 1, is characterized in that, the cave mouth diameter of the cave basin of growing seedlings that 2. step adopted is that 6cm, the degree of depth are 11cm.

6. a kind of Wand Cutting Techniques of Cyclocarya paliurus method according to claim 1, is characterized in that, step 3. in the base portion of the cuttings soak time of being stained with mud dipping in growth hormone solution be 60min.