CN107810677A - A kind of stripping and slicing rudiment method of Jiang He category flowers bulb - Google Patents

A kind of stripping and slicing rudiment method of Jiang He category flowers bulb Download PDF

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Publication number
CN107810677A
CN107810677A CN201710958870.3A CN201710958870A CN107810677A CN 107810677 A CN107810677 A CN 107810677A CN 201710958870 A CN201710958870 A CN 201710958870A CN 107810677 A CN107810677 A CN 107810677A
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stripping
slicing
bulb
jiang
rudiment
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CN107810677B (en
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丁华侨
刘建新
徐笑寒
毛俐慧
田丹青
王炜勇
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ZHEJIANG PROVINCE XIAOSHAN COTTON AND FLAX RESEARCH INSTITUTE
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ZHEJIANG PROVINCE XIAOSHAN COTTON AND FLAX RESEARCH INSTITUTE
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08—Immunising seed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a kind of stripping and slicing rudiment method of Jiang He category flowers bulbs.Step 1）Preservation, screening and the processing of bulb；Step 2）The cutting of bulb：Stripping and slicing is carried out to bulb, each stripping and slicing requirement at least contains a bud point；Step 3）Sterilization, desinsection and the wound healing processing of stripping and slicing；Step 4）The vernalization of stripping and slicing：The bulb stripping and slicing disinfected uniformly is placed in culture plate, it is covered with the matrix with moisture-retaining capacity of the sterilized processing of 1cm, be then placed into 25 DEG C 35 DEG C, vernalization is handled in the environment of humidity 80% or so, by the vernalization of 30 40 days, you can rudiment stripping and slicing of being unearthed；Step 5）The application of rudiment stripping and slicing：Rudiment stripping and slicing is used for tissue culture expanding propagation as explant, or rudiment stripping and slicing is cultivated and expands numerous into seedling, then transplanting production, progress nature growth.The present invention, which can make Jiang He belong to each bud point of flowers, to be sprouted, and greatly increase the breeding coefficient of Jiang He category flowers, the gardens flower border of boosting Jiang He category flowers, spend sea application and the popularization of new varieties.

Description

A kind of stripping and slicing rudiment method of Jiang He category flowers bulb
Technical field
The invention discloses a kind of induction Germination Technique of Jiang He category flowers bulb bud points, belong to plant culture and breeding skill Art field.
Background technology
Jiang He belongs to（Curcuma linnaeus, also known as turmeric）Plant is that one kind is new, has very much ornamental value Flowers, be perennial herb, have meat, fragrance rhizome, inflorescence is Long Circle or elliptical spike locusta, and sterile bract exists Arranged on inflorescence it is close, it is gorgeous and the duration is grown, great ornamental value.Including the curcuma alismatifolia (Curcuma that in the market is popular Alismatifolia Gagnep), beautiful comply with one's wishes（Curcuma hybrid ‘Laddawan’ ）, flourishing torch root tuber of aromatic turmeric（Curcuma hybrid ‘Red Torch’ ）, Saloman turmeric（Curcuma soloensis）, queen root tuber of aromatic turmeric（Curcuma petiolata ）Deng.
The production of Jiang He category flowers is mainly bred with napiform root, under field conditions (factors), December, as temperature drops Low and the sunshine-duration further to shorten, under ground portion starts to wither, and underground bulb then enters resting state, can now be planted The harvesting work of ball.For the reproductive number of every bulb typically at 3-5, breeding coefficient is relatively low.Especially developed in recent years Jiang He category flowers gardens flower border, spend sea apply since（Fourth overseas Chinese, Liu Jianxin, Zou Qingcheng etc., Jiang He category Flower Resources and gardens Applied analysis modern gardens, 2014,11（8）：85-88）, Jiang He category flowers in the winter time cold district by script perennial plant Thing becomes annual plant（Winter can not overwintering, bulb freezes to death）, Jiang He category flowers bulb production and supply become outstanding For anxiety, it is badly in need of improving breeding coefficient to alleviate the insufficient contradiction of bulb.Meanwhile it is new introduce Jiang He category flower varieties due to The limitation of quantity, can not Rapid Popularization into Landscape Application, cause gardens flower border application in Jiang He category flower variety it is relatively single One, thus the technical need for improving Jiang He category flowers breeding rates also seems very urgent.
Jiang He category flowers bulbs are generally a mother bulb band several nutrition balls（It is commonly called as milk container）, also some kinds are in mother bulb Lower section be extended with hyperplastic tissue（With bud point）.By taking the curcuma alismatifolia of the category as an example, curcuma alismatifolia bulb is symmetrical in the presence of 6 The bud point of arrangement.And other Jiang He category flowers, according to the of different sizes of bulb, existing bud point quantity also differs, and quantity is more Can reach more than ten such as flourishing torch root tuber of aromatic turmeric.Each bud possesses the potentiality sprouted into single plant in theory, but in reality In production, common Jiang He category flowers rudiments, all it is that a bulb goes out a bud, extremely there are two buds to occur individually.
The content of the invention
The invention provides a kind of stripping and slicing rudiment method of efficiently easy Jiang He category flowers bulbs, tissue culture can apply to Expand bulb separation breeding that is numerous and growing naturally, be remarkably improved breeding rate.
A kind of stripping and slicing rudiment method of Jiang He category flowers bulb, step are as follows：
Step 1）Preservation, screening and the processing of bulb：Selected from upper annual production and intact Jiang He category flowers bulbs Healthy and strong bulb, is rinsed well standby with clear water；
Step 2）The cutting of bulb：Stripping and slicing is carried out to bulb with the scalpel sterilized, each stripping and slicing requirement at least contains one Bud point；
Step 3）Sterilization, desinsection and the wound healing processing of stripping and slicing；
Step 4）The vernalization of stripping and slicing：The bulb stripping and slicing disinfected uniformly is placed in culture plate, is covered with the sterilized places of 1cm The matrix with moisture-retaining capacity of reason, it is then placed into 25 DEG C -35 DEG C, vernalization is handled in the environment of humidity 80% or so, process The vernalization of 30-40 days, you can rudiment stripping and slicing of being unearthed；
Step 5）The application of rudiment stripping and slicing：Rudiment stripping and slicing is used for tissue culture expanding propagation as explant, or rudiment stripping and slicing is cultivated into small Seedling, then transplanting production, it is numerous to carry out nature growth expansion.
Step 2）Described in bulb cutting, each stripping and slicing volume not less than 0.5cm*0.5cm*0.5cm cube or The other shapes of close volume.
Step 3）Described in the sterilization of stripping and slicing, desinsection and wound healing processing, bulb stripping and slicing be soaked in 70%-80% wine 10s in essence, is then pulled out, treats that alcohol volatilization is complete, final concentration of 1000 times of carbendazim and 2000 times of phoxim insecticide mix Close solution to soak 10 minutes, airing, handled 20 seconds with 15% 2000 times of liquid leachings of pentac missible oil, solution immersion mixed above It can exchange with the order of 2000 times of liquid leaching processing of pentac missible oil, then be air-dried 4 hours in shady place.
Described Jiang He category flowers include curcuma alismatifolia, it is beautiful comply with one's wishes, flourishing torch, Saloman turmeric, queen root tuber of aromatic turmeric.
Beneficial effects of the present invention
We belong to flowers bulb to Jiang He and carry out stripping and slicing processing according to bud point, and other bud points are sprouted to release main bud using physical means Inhibitory action existing for hair, each bud point is set to develop into independent individual, to realize being doubled and redoubled for breeding coefficient.According to public affairs Literature Consult is opened, is not all reported at present in Jiang He belongs to flowers in the research of correlation.The present invention can make Jiang He category flowers Each bud point can be sprouted, and the bud point of sprouting can be used for tissue culture expanding propagation as explant, can also cutting rudiment Block is cultivated to be expanded numerous into seedling, then transplanting production, progress nature growth.The breeding of Jiang He category flowers can so be greatly increased Coefficient, the gardens flower border of boosting Jiang He category flowers, spend sea application and the popularization of new varieties.
1）The present invention can make it that the bud point overwhelming majority of Jiang He category flowers bulbs can be sprouted, different according to species, Bud point quantity is sprouted about between 5-12；
2）The bud point obtained using the present invention can be used for tissue cultures directly as explant；
3）Using the rudiment stripping and slicing that obtains of the present invention, can directly cultivate into seedling, then carry out nature growth expand it is numerous.
Brief description of the drawings
Fig. 1 is the bulb of Jiang He category curcuma alismatifolias；
Fig. 2 is the beautiful bulb as one wishes of Jiang He category；
Fig. 3 is the bulb of Jiang He genus solenopsis torches；
Fig. 4 is the curcuma alismatifolia bulb stripping and slicing of budding；
Fig. 5 is the jade bulb stripping and slicing as one wishes of budding；
Fig. 6 is the flourishing torch bulb stripping and slicing of budding.
Embodiment
The present invention is further illustrated below by way of embodiments and drawings, but present disclosure is not limited to This.
Embodiment one
Step 1）Preservation, screening and the processing of bulb：Stalwartness is selected from upper annual production and intact curcuma alismatifolia bulb Bulb（As shown in Figure 1）, rinsed well with clear water.
Step 2）The cutting of bulb：Stripping and slicing is carried out to bulb with the scalpel sterilized, requirement at least contains per fritter tissues There is a bud point, stripping and slicing size is not less than 0.5cm*0.5cm*0.5cm（Or the other shapes of close volume cut shape）.
Step 3）The wound healing of stripping and slicing：Stripping and slicing is put in the good indoor environment of air flow property, section is exposed, cool It is dry 4 hours, to cicatrize a wound.
Step 4）The vernalization of stripping and slicing：The bulb stripping and slicing of airing is uniformly placed in culture plate, be covered with 1cm or so through going out The matrix for having certain moisture-retaining capacity of bacterium processing（Such as peat）, it is then placed into 25 DEG C -35 DEG C, in the environment of humidity 80% or so Vernalization was handled, by the vernalization of 30-40 days, you can germination of being unearthed；
Step 5）Hardening and transplanting：Stripping and slicing seedling is transferred in 25-32 DEG C of open-air atmosphere and carries out hardening 1 week, after robust seedling reality, Transplanted again, pay attention to that root can not be hindered during transplanting.
Step 6）Naturally growth and breeding：The management and maintenance of routine, the especially nutrient such as liquid manure are carried out to stripping and slicing seedling Will abundance, through outgrowth, bloom, aerial part it is gradually dead, then carry out bulb harvesting and storage.
As a result：Stripping and slicing bud point is sprouted normally, but bud ratio, about 80%, part diced facets have white mildew, and section occurs Go rotten, small part stripping and slicing softening, all rot, the bud point sprouted is then dead.
Embodiment two
Step 1）Preservation, screening and the processing of bulb：Stalwartness is selected from upper annual production and intact curcuma alismatifolia bulb Bulb, rinsed well with clear water.
Step 2）The cutting of bulb：Stripping and slicing is carried out to bulb with the scalpel sterilized, requirement at least contains per fritter tissues There is a bud point, stripping and slicing size is not less than 0.5cm*0.5cm*0.5cm（Or the other shapes of close volume cut shape）.
Step 3）The wound healing of stripping and slicing：Stripping and slicing is put in the good indoor environment of air flow property, section is exposed, cool It is dry 4 hours, to cicatrize a wound.
Step 4）The vernalization of stripping and slicing：The bulb stripping and slicing of airing is uniformly placed in culture plate, be covered with 1cm or so through going out The matrix for having certain moisture-retaining capacity of bacterium processing（Such as peat）, it is then placed into 25 DEG C -35 DEG C, in the environment of humidity 80% or so Vernalization was handled, by the vernalization of 30-40 days, you can germination of being unearthed.
Step 5）The bud point of sprouting is used for tissue culture expanding propagation as explant, i.e., by inoculation, culture, adventitious bud inducing, no Bud multiplication, small seedling rooting, hardening domestication carry out nature growth and breeding with transplanting and other steps（Particular content refers to, curcuma alismatifolia Quick breeding method for tissue culture, ZL201110177559.8）.
As a result：Stripping and slicing bud point is sprouted normally, but bud ratio, about 80%, part diced facets have white mildew, and section occurs Go rotten, small part stripping and slicing softening, all rot, the bud point sprouted is then dead.
Embodiment three
Step 1）Preservation, screening and the processing of bulb：Stalwartness is selected from upper annual production and intact curcuma alismatifolia bulb Bulb, rinsed well with clear water.
Step 2）The cutting of bulb：Stripping and slicing is carried out to bulb with the scalpel sterilized, requirement at least contains per fritter tissues There is a bud point, stripping and slicing size is not less than 0.5cm*0.5cm*0.5cm（Or the other shapes of close volume cut shape）.
Step 3）Sterilization, the desinsection processing of stripping and slicing：Bulb stripping and slicing is soaked in 10s in 70%-80% wine clear, then dragged for Go out, treat that alcohol volatilization is complete, then soaked with final concentration of 1000 times of carbendazim and 2000 times of phoxim insecticide mixed solution 10 minutes, airing, handled 20 seconds with 15% 2000 times of liquid leachings of pentac missible oil, sequence described above can exchange.Then in cool place Place air-dries 4 hours.
Step 4）The vernalization of stripping and slicing：The bulb stripping and slicing disinfected uniformly is placed in culture plate, is covered with 1cm or so Sterilized processing the matrix for having certain moisture-retaining capacity（Such as peat）, it is then placed into 25 DEG C -35 DEG C, humidity 80% or so Vernalization was handled in environment, by the vernalization of 30-40 days, you can germination of being unearthed（As shown in Figure 4）.
Step 5）Hardening and transplanting：Stripping and slicing seedling is transferred in more than 25-32 DEG C of open-air atmosphere and carries out hardening 1 week, is treated After robust seedling reality, then transplanted, pay attention to that root can not be hindered during transplanting.
Step 6）Naturally growth and breeding：The management and maintenance of routine, the especially nutrient such as liquid manure are carried out to stripping and slicing seedling Will abundance, through outgrowth, bloom, aerial part it is gradually dead, then carry out bulb harvesting and storage.
As a result：Stripping and slicing bud point is sprouted normally, and for bud ratio about in 95%, averagely often bulb acquisition budlet 5.7, stripping and slicing is normal, Without obvious putrefactive phenomenon.
Example IV
Step 1）Preservation, screening and the processing of bulb：Stalwartness is selected from upper annual production and intact curcuma alismatifolia bulb Bulb, rinsed well with clear water.
Step 2）The cutting of bulb：Stripping and slicing is carried out to bulb with the scalpel sterilized, requirement at least contains per fritter tissues There is a bud point, stripping and slicing size is not less than 0.5cm*0.5cm*0.5cm（Or the other shapes of close volume cut shape）.
Step 3）Sterilization, the desinsection processing of stripping and slicing：Bulb stripping and slicing is soaked in 10s in 70%-80% wine clear, then dragged for Go out, treat that alcohol volatilization is complete, then soaked with final concentration of 1000 times of carbendazim and 2000 times of phoxim insecticide mixed solution 10 minutes, airing；Handled 20 seconds with 15% 2000 times of liquid leachings of pentac missible oil, sequence described above can exchange.Then in cool place Place air-dries 4 hours.
Step 4）The vernalization of stripping and slicing：The bulb stripping and slicing disinfected uniformly is placed in culture plate, is covered with 1cm or so Sterilized processing the matrix for having certain moisture-retaining capacity（Such as peat）, it is then placed into 25 DEG C -35 DEG C, humidity 80% or so Vernalization was handled in environment, by the vernalization of 30-40 days, you can germination of being unearthed.
Step 5）The bud point of sprouting is used for tissue culture expanding propagation as explant, i.e., by inoculation, culture, adventitious bud inducing, no Bud multiplication, small seedling rooting, hardening domestication carry out nature growth and breeding with transplanting and other steps.
As a result：Stripping and slicing bud point is sprouted normally, and for bud ratio about in 95%, averagely often bulb acquisition budlet 5.7, stripping and slicing is normal, Without obvious putrefactive phenomenon.
Embodiment five
Step 1）Preservation, screening and the processing of bulb：Stalwartness is selected from upper annual production and intact jade bulb as one wishes Bulb（As shown in Figure 2）, rinsed well with clear water.
Step 2）The cutting of bulb：Stripping and slicing is carried out to bulb with the scalpel sterilized, requirement at least contains per fritter tissues There is a bud point, stripping and slicing size is not less than 0.5cm*0.5cm*0.5cm（Or the other shapes of close volume cut shape）.
Step 3）Sterilization, the desinsection processing of stripping and slicing：Bulb stripping and slicing is soaked in 10s in 70%-80% wine clear, then dragged for Go out, treat that alcohol volatilization is complete, then soaked with final concentration of 1000 times of carbendazim and 2000 times of phoxim insecticide mixed solution 10 minutes, airing；Handled 20 seconds with 15% 2000 times of liquid leachings of pentac missible oil, sequence described above can exchange.Then in cool place Place air-dries 4 hours.
Step 4）The vernalization of stripping and slicing：The bulb stripping and slicing disinfected uniformly is placed in culture plate, is covered with 1cm or so Sterilized processing the matrix for having certain moisture-retaining capacity（Such as peat）, it is then placed into 25 DEG C -35 DEG C, humidity 80% or so Vernalization was handled in environment, by the vernalization of 30-40 days, you can germination of being unearthed（As shown in Figure 5）.
Step 5）Hardening and transplanting：Stripping and slicing seedling is transferred in more than 25-32 DEG C of open-air atmosphere and carries out hardening 1 week, is treated After robust seedling reality, then transplanted, pay attention to that root can not be hindered during transplanting.
Step 6）Naturally growth and breeding：The management and maintenance of routine, the especially nutrient such as liquid manure are carried out to stripping and slicing seedling Will abundance, through outgrowth, bloom, aerial part it is gradually dead, then carry out bulb harvesting and storage.
As a result：Stripping and slicing bud point is sprouted normally, and bud ratio is about in 93%, averagely often bulb acquisition budlet 5.93, and stripping and slicing is just Often, without obvious putrefactive phenomenon.
Embodiment six
Step 1）Preservation, screening and the processing of bulb：Stalwartness is selected from upper annual production and intact jade bulb as one wishes Bulb, rinsed well with clear water.
Step 2）The cutting of bulb：Stripping and slicing is carried out to bulb with the scalpel sterilized, requirement at least contains per fritter tissues There is a bud point, stripping and slicing size is not less than 0.5cm*0.5cm*0.5cm（Or the other shapes of close volume cut shape）.
Step 3）Sterilization, the desinsection processing of stripping and slicing：Bulb stripping and slicing is soaked in 10s in 70%-80% wine clear, then dragged for Go out, treat that alcohol volatilization is complete, then soaked with final concentration of 1000 times of carbendazim and 2000 times of phoxim insecticide mixed solution 10 minutes, airing；Handled 20 seconds with 15% 2000 times of liquid leachings of pentac missible oil, sequence described above can exchange.Then in cool place Place air-dries 4 hours.
Step 4）The vernalization of stripping and slicing：The bulb stripping and slicing disinfected uniformly is placed in culture plate, is covered with 1cm or so Sterilized processing the matrix for having certain moisture-retaining capacity（Such as peat）, it is then placed into 25 DEG C -35 DEG C, humidity 80% or so Vernalization was handled in environment, by the vernalization of 30-40 days, you can germination of being unearthed.
Step 5）The bud point of sprouting is used for tissue culture expanding propagation as explant, i.e., by inoculation, culture, adventitious bud inducing, no Bud multiplication, small seedling rooting, hardening domestication carry out nature growth and breeding with transplanting and other steps（Particular content refers to, curcuma alismatifolia Quick breeding method for tissue culture, ZL201110177559.8）.
As a result：Stripping and slicing bud point is sprouted normally, and bud ratio is about in 93%, averagely often bulb acquisition budlet 5.93, and stripping and slicing is just Often, without obvious putrefactive phenomenon.
Embodiment seven
Step 1）Preservation, screening and the processing of bulb：Stalwartness is selected from upper annual production and intact flourishing torch bulb Bulb（As shown in Figure 3）, rinsed well with clear water.
Step 2）The cutting of bulb：Stripping and slicing is carried out to bulb with the scalpel sterilized, requirement at least contains per fritter tissues There is a bud point, stripping and slicing size is not less than 0.5cm*0.5cm*0.5cm（Or the other shapes of close volume cut shape）.
Step 3）Sterilization, the desinsection processing of stripping and slicing：Bulb stripping and slicing is soaked in 10s in 70%-80% wine clear, then dragged for Go out, treat that alcohol volatilization is complete, then soaked with final concentration of 1000 times of carbendazim and 2000 times of phoxim insecticide mixed solution 10 minutes, airing；Handled 20 seconds with 15% 2000 times of liquid leachings of pentac missible oil, sequence described above can exchange.Then in cool place Place air-dries 4 hours.
Step 4）The vernalization of stripping and slicing：The bulb stripping and slicing disinfected uniformly is placed in culture plate, is covered with 1cm or so Sterilized processing the matrix for having certain moisture-retaining capacity（Such as peat）, it is then placed into 25 DEG C -35 DEG C, humidity 80% or so Vernalization was handled in environment, by the vernalization of 30-40 days, you can germination of being unearthed（As shown in Figure 6）.
Step 5）Hardening and transplanting：Stripping and slicing seedling is transferred in more than 25-32 DEG C of open-air atmosphere and carries out hardening 1 week, is treated After robust seedling reality, then transplanted, pay attention to that root can not be hindered during transplanting.
Step 6）Naturally growth and breeding：The management and maintenance of routine, the especially nutrient such as liquid manure are carried out to stripping and slicing seedling Will abundance, through outgrowth, bloom, aerial part it is gradually dead, then carry out bulb harvesting and storage.
As a result：Stripping and slicing bud point is sprouted normally, and bud ratio is about in 95%, averagely often bulb acquisition budlet 11.1, and stripping and slicing is just Often, without obvious putrefactive phenomenon.
Embodiment eight
Step 1）Preservation, screening and the processing of bulb：Stalwartness is selected from upper annual production and intact flourishing torch bulb Bulb, rinsed well with clear water.
Step 2）The cutting of bulb：Stripping and slicing is carried out to bulb with the scalpel sterilized, requirement at least contains per fritter tissues There is a bud point, stripping and slicing size is not less than 0.5cm*0.5cm*0.5cm（Or the other shapes of close volume cut shape）.
Step 3）Sterilization, the desinsection processing of stripping and slicing：Bulb stripping and slicing is soaked in 10s in 70%-80% wine clear, then dragged for Go out, treat that alcohol volatilization is complete, then soaked with final concentration of 1000 times of carbendazim and 2000 times of phoxim insecticide mixed solution 10 minutes, airing；Handled 20 seconds with 15% 2000 times of liquid leachings of pentac missible oil, sequence described above can exchange.Then in cool place Place air-dries 4 hours.
Step 4）The vernalization of stripping and slicing：The bulb stripping and slicing disinfected uniformly is placed in culture plate, is covered with 1cm or so Sterilized processing the matrix for having certain moisture-retaining capacity（Such as peat）, it is then placed into 25 DEG C -35 DEG C, humidity 80% or so Vernalization was handled in environment, by the vernalization of 30-40 days, you can germination of being unearthed.
Step 5）The bud point of sprouting is used for tissue culture expanding propagation as explant, i.e., by inoculation, culture, adventitious bud inducing, no Bud multiplication, small seedling rooting, hardening domestication carry out nature growth and breeding with transplanting and other steps（Particular content refers to, curcuma alismatifolia Quick breeding method for tissue culture, ZL201110177559.8）.
As a result：Stripping and slicing bud point is sprouted normally, and bud ratio is about in 95%, averagely often bulb acquisition budlet 11.1, and stripping and slicing is just Often, without obvious putrefactive phenomenon.

Claims (4)

  1. A kind of 1. stripping and slicing rudiment method of Jiang He category flowers bulb, it is characterised in that step is as follows：
    Step 1）Preservation, screening and the processing of bulb：Selected from upper annual production and intact Jiang He category flowers bulbs Healthy and strong bulb, is rinsed well standby with clear water；
    Step 2）The cutting of bulb：Stripping and slicing is carried out to bulb with the scalpel sterilized, each stripping and slicing requirement at least contains one Bud point；
    Step 3）Sterilization, desinsection and the wound healing processing of stripping and slicing；
    Step 4）The vernalization of stripping and slicing：The bulb stripping and slicing disinfected uniformly is placed in culture plate, is covered with the sterilized places of 1cm The matrix with moisture-retaining capacity of reason, it is then placed into 25 DEG C -35 DEG C, vernalization is handled in the environment of humidity 80% or so, process The vernalization of 30-40 days, you can rudiment stripping and slicing of being unearthed；
    Step 5）The application of rudiment stripping and slicing：Rudiment stripping and slicing is used for tissue culture expanding propagation as explant, or rudiment stripping and slicing is cultivated into small Seedling, then transplanting production, it is numerous to carry out nature growth expansion.
  2. 2. the stripping and slicing rudiment method of Jiang He category flowers bulb according to claim 1, it is characterised in that step 2）Described in Bulb cutting, it is characterised in that each cube of the stripping and slicing volume not less than 0.5cm*0.5cm*0.5cm or close volume Other shapes.
  3. 3. the stripping and slicing rudiment method of Jiang He category flowers bulb according to claim 1, it is characterised in that step 3）Described in The sterilization of stripping and slicing, desinsection and wound healing processing, bulb stripping and slicing be soaked in 10s in 70%-80% alcohol, then pull out, treat Alcohol volatilization is complete, and final concentration of 1000 times of carbendazim and 2000 times of phoxim insecticide mixed solution soak 10 minutes, cool It is dry, handled 20 seconds with 15% 2000 times of liquid leachings of pentac missible oil, solution immersion mixed above and 2000 times of liquid of pentac missible oil The order of leaching processing can exchange, and then be air-dried 4 hours in shady place.
  4. 4. the stripping and slicing rudiment method of Jiang He category flowers bulb according to claim 1, it is characterised in that described Jiang He category Flowers include curcuma alismatifolia, it is beautiful comply with one's wishes, flourishing torch, Saloman turmeric, queen root tuber of aromatic turmeric.
CN201710958870.3A 2017-10-16 2017-10-16 Cutting and sprouting method for zingiber flower bulbs Expired - Fee Related CN107810677B (en)

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CN113796297A (en) * 2020-07-30 2021-12-17 佛山市连艺生物科技有限公司 Cultivation method for improving water culture quality of curcuma longa
CN113950889A (en) * 2021-10-19 2022-01-21 绍兴文理学院元培学院 Making method of artificial seeds of curcuma alismatifolia
CN114938779A (en) * 2022-06-15 2022-08-26 闽南师范大学 Method for establishing curcuma alismatifolia regeneration system by taking florets as explants

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113796297A (en) * 2020-07-30 2021-12-17 佛山市连艺生物科技有限公司 Cultivation method for improving water culture quality of curcuma longa
CN113796297B (en) * 2020-07-30 2022-06-07 佛山市连艺生物科技有限公司 Cultivation method for improving water culture quality of curcuma longa
CN113950889A (en) * 2021-10-19 2022-01-21 绍兴文理学院元培学院 Making method of artificial seeds of curcuma alismatifolia
CN114938779A (en) * 2022-06-15 2022-08-26 闽南师范大学 Method for establishing curcuma alismatifolia regeneration system by taking florets as explants
CN114938779B (en) * 2022-06-15 2023-08-18 闽南师范大学 Method for establishing ginger lotus regeneration system by taking floret as explant

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