CN103430903B - Method for preparing non-human animal model with opportunistic infections and chronic wasting diseases and medicament screened by using same - Google Patents
Method for preparing non-human animal model with opportunistic infections and chronic wasting diseases and medicament screened by using same Download PDFInfo
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- CN103430903B CN103430903B CN201310409648.XA CN201310409648A CN103430903B CN 103430903 B CN103430903 B CN 103430903B CN 201310409648 A CN201310409648 A CN 201310409648A CN 103430903 B CN103430903 B CN 103430903B
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Abstract
The invention discloses a method for establishing an animal model with opportunistic infections and chronic wasting diseases, and a traditional Chinese medicinal preparation for treating opportunistic infections and chronic wasting diseases. The traditional Chinese medicinal preparation is prepared by the following components in parts by weight: 100-180 parts of monkhood, 100-180 parts of dry ginger, and 100-160 parts of liquorice. The method has the positive effects that the method for establishing the animal model with opportunistic infections and chronic wasting diseases is found, and the model has high controllability, reliability and repeatability, and is convenient and economical to use; the model syndrome accords with the attack process of opportunistic infections and chronic wasting diseases; the model establishing factor accords with the theory of the Internal Canon of Medicine, and does not relates to causal organisms, so that the testing risks can be reduced, and the organism safety can be guaranteed; the traditional Chinese medicinal preparation obtained by screening has the characteristics of high efficiency, no toxicity, wide medicine sources and the like, is convenient to use and economical, can be used for prolonging the life of patients with opportunistic infections and chronic wasting diseases, improving the living quality of the patients and improving lesion of each tissue, and does not have toxic or side effect when a large dosage is used.
Description
Technical field
The present invention relates to a kind of non-human animal model, specifically the method for draft machine opportunistic infections and chronic wasting disease non-human animal model.The invention still further relates to the method utilizing this model discrimination, and the medicine of the therapy apparatus opportunistic infections screened and chronic wasting disease.
Background technology
Opportunistic infections refer to the pathogene that some pathogenicities are more weak, can not cause a disease when immune function of human body is normal, but when immune function of human body reduces, they are taken advantage of a weak point, and invade in human body, cause various disease.Normal flora is low at body's immunity, also can cause infection under the specified conditions such as position change or flora imbalance of living away from home.Be found in following situation: the surface barrier effect of (1) skin and mucous membrane is damaged, as damage, burn, intubate, operation etc.(2) congenital immune deficiency.Comprise: 1. humoral immune defect, often repeated infection occurs as congenital without gamma globulin mass formed by blood stasis patient, especially bacteriological infection., as thymic aplasia or hypogenetic patient, very easily there is virus, tulase or fungal infection in the 2. patient of cellular immune function defect, also easily suffers from parasitosis as Pneumocystis Carinii Infection In Rats.3. Reconstruction in Sever Combined Immunodeciency disease, the T cell of patient and the immunologic function of B cell lack completely, often die from infection in 1 year after birth.(3) secondary immunodeficiency.As the patient of patients with advanced cancer, application immunodepressant steroid hormone, antimetabolite, alkylating agent and antifol etc., person in poor health and chronic disease patient, some infects patient as measles, infectious mononucleosis etc., normal because of immunocyte decline, easily infect.(4) acquired immunodeficiency syndrome (AIDS).AIDS is that be made up of the multiple metabolic disorder and immune disorder involving whole body multisystem multiple organ, patient often dies from the expendable cachexia after opportunistic infections and metabolism severe disorder because immunity of organism is low or defect.Human immunodeficiency virus (HIV) and non-HIV virus infections all can cause AIDS, can involve the organization systems such as eye, skin, urinary system, respiratory system, digestive system, central nervous system.HIV virus mainly invades helper lymphocyte T, and the quantity of this cell is obviously lowered, and infected tract is extensive, and the infected chance of patient is frequent, and patient nearly all dies from secondary infection and tumour.HAART therapy at present, causes the treatment of acquired immunodeficiency syndrome for HIV, often adopts highly active antiretroviral therapy (HAART), although can make blood plasma HIV carrying capacity and the CD4 of patient
+t subset lymphocyte count is improved, but most of patients occurs that clinical symptoms worsens, even dead.Current, the generation of the acquired immunodeficiency syndrome that HIV causes is still in quick growth, the key character that the incidence of disease is high, pathogenesis is unclear, the form of expression is various, treatment is acquired immunodeficiency syndrome without specificity, has become the hot subject of domestic and international acquired immune deficiency syndrome (AIDS) research.In addition, the producing cause of the acquired immunodeficiency syndrome that non-HIV virus factor causes, Forming Mechanism also do not understand, its treatment is also problem demanding prompt solution.(5) some antibiotic prolonged application, can suppress normal flora, and this is conducive to the growth of some conditioned pathogen as fungi, thus causes opportunistic infections.(6) immunity superfluous (autoimmune disease).Infect multiple be born in autoimmune disease morbidity for many years before.The aggregate measures such as clinical normal employing immunodepressant and anti-infectives, but easily cause patient immune function to decline, bring out opportunistic infections.(7) organ transplant is postoperative.Clinically, for suppressing the posttransplantation HVGR of organ and graft-versus-host reaction, conventional immunosuppressive drug, but easily bring out infection.In above-mentioned situation, patient clinical often shows as repeated infection or serious infections, even causes death.For above-mentioned opportunistic infections, clinical major measure treatment is: (1) eliminating pathogen.Conventional anti-infectives, as antibacterials, antifungal, antiviral agent etc., but the application of anti-infectious agent can bring in various degree with the bad reaction of type, as " immunologic reconstitution inflammatory syndrome (IRIS) " that the suprainfection of the prolonged application of drug resistance, broad spectrum antibiotic, the antiretroviral therapy (ART) of AIDS occur afterwards---Novel presentation, vital tissue organ damage etc. that the clinical symptoms occurred in body's immunity process of reconstruction worsens.(2) manage to improve the immunologic function of human body, as strengthened nutrition, defeated fresh blood or leucocyte, injection gamma globulin, thymic peptide, specific immune ribonucleic acid etc., but clinical efficacy is limited and bring certain bad reaction.Therefore, exploitation promotes body's immunity reconstruction, low toxicity or nontoxic medicine, is expected to the effect improving therapy apparatus opportunistic infections.
Chronic wasting disease generally refers to that classes such as various malignant tumour, pulmonary tuberculosis, atrophic gastritis, severe trauma, burn, systemic loupus erythematosus, chronic suppurative infection, chronic blood loss consume body energy material excessively, causes the disease general name of human body energy negative balance.Show as apositia, atrophic gastritis, chronic colitis, chronic hepatitis, tuberculosis, tumour (especially malignant tumour), hyperthyroidism, diabetes etc.Often there is cachexia in patient, skeletonize, finally because of excessively weak and dead.Clinical treatment measure is: (1) for nutrition intake deficiency, apositia, the patient such as excessively to go on a diet for a long time, often adopts the measure increasing patient's nutrition supply; (2) for patients such as atrophic gastritis, chronic colitis, chronic hepatitiss, adopt and improve nutrient absorption, the measure such as antibacterial; (3) for the patient such as tuberculosis, tumour, adopt antibacterial, antineoplaston measures such as () chemotherapy, radiotherapy, operations and reduce the measures such as patient's nutrient consumption; (4) for the patient such as hyperthyroidism, diabetes, the remedy measures improving patient's metabolic function is often taked.Above-mentioned clinical treatment measure often can cause multiple bad reaction, causes the clinical efficacy of chronic wasting disease still undesirable.Therefore, research and development are efficient, the medicine of low toxicity or nontoxic treatment chronic wasting disease, become the task of top priority.
Research of Animal Model for Study opportunistic infections and chronic wasting disease is adopted to carry out studying more easily realizing than adopting human trial.Utilize suitable animal opportunistic infections and chronic wasting disease model to carry out controllability, reliability, the repeatability of opportunistic infections and chronic wasting disease research tool height, can flow of research be shortened simultaneously, reduce disturbing factor, reduce empirical risk.Opportunistic infections and the chronic wasting disease Research of Animal Model for Study aspect with TCM Syndrome Features belong to blank, and the science objective evaluation of sifting machine opportunistic infections and chronic wasting disease medicine and curative effect brings obvious obstacle.
Summary of the invention
The invention reside in the method that draft machine opportunistic infections and chronic wasting disease non-human animal model are provided.
The technical scheme realizing the object of the invention is: comprise following steps:
Comprise following steps:
(1) by every male non-human animal in every day 18:00 and 6-10 only, female non-human animal mates, and makes it mating,
(2) tri-time periods of next day 9:00-9:30,12:30-13:00,17:00-17:30, above-mentioned male non-human animal is swum, gives feeding after each swimming terminates less than or equal to the distilled water of 4 degrees Celsius or cold water,
(3) continuous 7 days,
(4) pick out now state symptom non-human animal: illness in eye+depilation+abdominal distension diarrhoea+blood urine+opportunistic infect.
Wherein,
Described non-human animal is selected from: rat, mouse;
Described opportunistic infect comprises: streptococcus pneumonia, staphylococcus aureus, bacillus variabilis, Candida albicans, kidney mycotic infection and/or enteron aisle infection by Strongyloides stercoralis;
Described opportunistic infect is found by Pathogen test.
General mouse is more convenient, so the preferably scheme realizing the object of the invention is, (1): by the male mouse of cleaning grade at every only 1 monthly age in every day 18:00 and 6-10 female mice mate, and make it mating; (2): tri-time periods of next day 9:00-9:30,12:30-13:00,17:00-17:30, above-mentioned male mouse is swum, the distilled water less than or equal to 4 degrees Celsius or cold water 3ml is given after each swimming terminates, (3) continuous 7 days, (4) pick out now state symptom male mouse: the diarrhoea+blood urine+opportunistic infect of disease+depilation+abdominal distension.
Method of the present invention is to occur that " illness in eye+depilation+abdominal distension diarrhoea+blood urine+opportunistic infect " is as modeling success.
The theoretical foundation that this model is set up is:
According to pathological process and the clinical symptoms of opportunistic infections and chronic wasting disease, opportunistic infections and chronic wasting disease should belong to the category such as the traditional Chinese medical science " epidemic disease ", " consumptive disease ".Occur opportunistic infections and chronic wasting disease external cause mainly catching pestilential pathogen, damage body healthy tendency, internal cause is that vital essence is taken by force and caused healthy tendency to be consumed, and qi and blood is all empty, and with the passing of time then the five internal organs are all empty, finally causes vital QI cannot conquer pathogen, and its disease is from life.We are theoretical according to the "Nei Jing" of " consumption of normal qi causing asthenia → pathogenic domination inducing sthenia → then progress of disease of struggling between the vital energy and the pathogenic factor ", establish the mouse model of symbol opportunistic infections and chronic wasting disease.
The method of draft machine opportunistic infections of the present invention and chronic wasting disease non-human animal model, its advantage is: the 1. controllability of this model tool height, reliability, repeatability and easy to use, economic; 2. this model syndrome meets clinical onset process; 3. modeling factor meets "Nei Jing" theory; 4. this model is set up does not need to use causal organism infection animal, reduces empirical risk, ensures bio-safety.
Another object of the present invention is to provide the method for the medicine of screening therapy apparatus opportunistic infections and chronic wasting disease, and the method utilizes the non-human animal model prepared according to preceding method, comprises the steps:
(1) with alternative material to the non-human animal model administration prepared according to preceding method, and
(2) observe, check and/or detect the opportunistic infections of this animal model and the change of chronic wasting disease situation,
(3) picking out can mitigation symptoms, life-extending and/or reduce the material of lethality, the effective ingredient of the medicine in this, as therapy apparatus opportunistic infections and chronic wasting disease or the medicine as therapy apparatus opportunistic infections and chronic wasting disease.
Wherein said alternative material comprises: cmm of simple prescription, Chinese medicine compound prescription, compound, composition.Here, Chinese medicine comprises ethnic drug, as: Tibetan medicine, anaesthetic, seedling medicine, precious jade medicine, strong medicine, Dai Nationality's medicine, Yi nationality's medicine, the She nationality, distributed over Fujian, Zhejiang, Jiangxi and Guangdong's medicine, dimension medicine.
Wherein said administration is: connect upright for alternatives or be processed into the form being convenient to administration, is entered in this animal model by mode that is oral or injection.In addition any administering mode be applicable to can also be taked.
In the present invention, the method of the medicine of screening therapy apparatus opportunistic infections and chronic wasting disease, in principle, on conventional method and whole concerns of all medicament research and development screening of concrete process, experiment, detection, medicine preparation, formulation dosage, medication etc., all with specification, routine or method that laws and regulations specify and require consistent.
3rd object of the present invention is to provide a kind of Chinese medicine compound prescription being used for the treatment of opportunistic infections and chronic wasting disease.This Chinese medicine compound prescription utilizes the non-human animal model prepared according to preceding method, is obtained by aforesaid screening technique.
This Chinese medicine compound prescription, is made up of following raw material by weight ratio: monkshood 100 ~ 180 parts, rhizoma zingiberis 100 ~ 180 parts, 100 ~ 160 parts, Radix Glycyrrhizae.
The preferred weight of this Chinese medicine compound prescription is: monkshood 150 parts, rhizoma zingiberis 150 parts, 150 parts, Radix Glycyrrhizae.
Described raw material is dry product.
This Chinese medicine compound prescription can be prepared by the method comprised the steps:
(1) traditional Chinese medicine is taken according to bulk drug part by weight, cataclasm;
(2) add medicinal material total amount 5 ~ 9 times of water gagings, boil rear little fire and extract 3 ~ 6 hours;
(3) filter and remove residue, obtains Aqueous extracts, and gained Aqueous extracts is the Chinese medicine compound prescription being used for the treatment of opportunistic infections and chronic wasting disease.
By above-mentioned Aqueous extracts medicine manufacture method conveniently, making with it is that the oral liquid of whole composition or partial elements or injection, tablet, capsule, granular medicine are with therapy apparatus opportunistic infections and chronic wasting disease.
The pharmacological action of this Chinese medicine compound prescription each component is:
Monkshood: pungent, sweet is warm greatly; Poisonous.The thoughts of returning home, kidney, the spleen channel.There is recuperating depleted yang, mend fire supporing yang, by effect of wind-cold damp pathogen.Cure mainly yang depletion collapse, the cold weak pulse of limb, impotence, Gong Leng, trusted subordinate's crymodynia, the cold of insufficiency type is vomited and diarrhoea, cold sensation of the genitalia oedema, numbness and ache of cold dampness etc." book on Chinese herbal medicine converges and says " cloud: " monkshood, returns yang-energy, loose cold sensation of the genitalia.The time of all genus deficiency of yang negative electrodes, lung nephrons heat symptom-complex person, clothes had dead distinguished service." for yin excess gram sun, loss of YANG due to profuse perspiration, vomiting and diarrhoea, it is inverse to faint, the cold weak pulse of limb, trusted subordinate's crymodynia, cold dysentery, beriberi oedema, wind-cold-dampness arthralgia, impotence, Gong Leng, and the cold of insufficiency type is vomited and diarrhoea, cold sensation of the genitalia oedema, deficiency of yang diseases caused by external factors, the disease of cloudy subcutaneous ulcer sore and all chronic diseaseses.And monkshood is to hypophysis---interrenal system has excitation, can also excited vagus and tool cardiotonic.Pharmacological action: the effect such as function, cold resistance of cardiac stimulant, anti-arrhythmia, Hemorrhagic shock, hemangiectasis, increase CBF, enhancing interrenal system; And anti-inflammatory, analgesia, cold resistance, raising are to effects such as the tolerances of anoxic.
Rhizoma zingiberis: taste is pungent, hot in nature.Return spleen, stomach, the heart, lung channel.There is warming spleen and stomach for dispelling cold, Hui Yang promote blood circulation, eliminating dampness dissolving phlegm, warming lung to reduce watery phlegm effect.Cure mainly coldness and pain in the epigastrium, vomit, have loose bowels, the cold weak pulse of limb, yang depletion are fainted, and inverse, cold drink is breathed with cough, numbness and ache of cold dampness.Pharmacological action: have excited guinea pig in vitro intestinal tube, excited vasomotor center and heart respiratory center, excited heart, antihistamine, antibacterial, suppress the effect such as platelet aggregation.
Radix Glycyrrhizae: taste is sweet, property is put down, nontoxic.Have beneficial gas bowl spares, relieving spasm to stop pain, moisten the lung and relieve the cough, purging intense heat and detonicating, coordinating the drug actions of a prescription, diuresis effect.Cure mainly burnout food less, flesh thin Huang, palpitation, stomachache loose stool, the anxious pain of four limbs contraction, hysteria, cough and asthma, abscess of throat, carbuncle sore swell and ache, infant fetal toxin and medicine, food poisoning.Also odynuria, carbuncle sore tumefacting virus and cushion toxicity, strong is drenched for weakness of the spleen and the stomach, lassitude hypodynamia, palpitation, coughing with a lot of sputum, the anxious pain of gastral cavity abdomen and four limbs contraction, heat.Pharmacological action: there is anti-inflammatory, antiviral, function such as treatment ulcer, protecting liver and detoxication, enhancing immunologic function, anti-cancer and cancer-preventing, interferon inducers and cell immunomodulator etc.Clinical treatment gastric and duodenal ulcer, various acute, chronic hepatitis, bronchitis, acquired immune deficiency syndrome (AIDS) and the antiatherosclerosis of being widely used in is formed.
Experiment confirms, of the present inventionly be used for the treatment of the therapy apparatus opportunistic infections of opportunistic infections and chronic wasting disease and the Chinese medicine compound prescription of chronic wasting disease, have and extend opportunistic infections and chronic wasting disease mouse life, reduction lethality, and opportunistic infections and the little rathole of chronic wasting disease, lung, liver, Shen Deng tissue pathologies change can be improved, and have no side effect after medication, be the Chinese medicine compound prescription that a kind of efficient, low toxicity, medicine source are wide, easy to use, economic.
Embodiment
Prepared by Chinese medicine compound prescription
Embodiment 1
Take: monkshood 150g, rhizoma zingiberis 150g, Radix Glycyrrhizae 150g.
Above-mentioned raw materials is dry product.
Then,
(1) cataclasm to 1 ~ 3mm;
(2) add water 2500g, boils rear little fire and extract 4 hours;
(3) filter and remove residue, obtains Aqueous extracts 450ml.I.e. every milliliter contained 1 gram.
Gained Aqueous extracts is the Chinese medicine compound prescription being used for the treatment of opportunistic infections and chronic wasting disease.
Embodiment 2
Take: monkshood 100g, rhizoma zingiberis 100g, Radix Glycyrrhizae 160g.
Above-mentioned raw materials is dry product.
Then,
(1) cataclasm to 1 ~ 3mm;
(2) add water 3200g, boils rear little fire and extract 6 hours;
(3) filter and remove residue, obtains Aqueous extracts 450ml.I.e. every milliliter contained 1 gram.
Gained Aqueous extracts is the Chinese medicine compound prescription being used for the treatment of opportunistic infections and chronic wasting disease.
Embodiment 3
Take: monkshood 180g, rhizoma zingiberis 180g, Radix Glycyrrhizae 100g.
Above-mentioned raw materials is dry product.
Then,
(1) cataclasm to 1 ~ 3mm;
(2) add water 3220g, boils rear little fire and extract 6 hours;
(3) filter and remove residue, obtains Aqueous extracts 450ml.I.e. every milliliter contained 1 gram.
Gained Aqueous extracts is the Chinese medicine compound prescription being used for the treatment of opportunistic infections and chronic wasting disease.
Modeling and pharmacotherapy
The male mouse 72 of Kunming kind, body weight 16.9 ± 2 g, at 1 monthly age, is provided by Medical Colleges Of Guilin's animal experimental center, credit number: GLMC20130308HL.
Male for the cleaning grade at 1 monthly age mouse is divided into 2 groups at random: 1. Normal group (CTRL, 24): tri-time periods of every day 9:00-9:30,12:30-13:00,17:00-17:30 swim, 28 DEG C of distilled water 3ml are given, continuous 7 days after each swimming terminates.2. model group (MAIDS, 48): modeling factor comprises " sexual strain is lost smart+tired+interior raw ", every day, every male mice and 6-10 female mice were first mated mating by 18:00, daytime next day carries out the swimming identical with control group, the distilled water 3ml less than or equal to 4 degrees Celsius is given, continuous 7 days after each swimming terminates.
After modeling success, model group is divided into not treatment group and compound of Chinese herbal medicine group two groups, often organizes 24.Treatment group does not refuse pharmacotherapy, every day gavage running water, every only 1 milliliter.
Compound of Chinese herbal medicine group gives the compound of Chinese herbal medicine with gained of the present invention.Chinese medicine compound prescription raw material is totally 450 grams of (monkshood 150g, rhizoma zingiberis 150g, Radix Glycyrrhizae 150g) add 2500 ml waters, decoct medicine soup 450 milliliters, every milliliter contained 1 gram, every day gavage once, each 1 milliliter, continuous treatment drug withdrawal after 7 days, observes mouse life, and the 28th day each changes in histopathology of detection mouse after administration.
Observe and find, Normal group 24 mouse all do not fall ill.
And model group 48 mouse 7 days occur afterwards: (1) illness in eye (eye conjunctiva and ulcer of the cornea, hemorrhage, suppurations → eyes close, blind); (2) (being equivalent to patient's erythematous rash) is lost hair or feathers; (3) digestive function not normal (serious abdominal distension, Mucous Stool); (4) the not normal deeline of metabolism such as blood urine; (5) become thin; (6) high contagiousness; (7) Pathogen test finds the opportunistic infects such as streptococcus pneumonia, staphylococcus aureus, bacillus variabilis, Candida albicans, mould, bulky stool quasi-colubriformis.The pathological change that model group mouse is respectively organized is: empsyxis oedema, enteron aisle strongyloides intestinalis, kidney mycotic infection.
Lethality not in treatment group morbidity mouse 7 days is up to 87.5%.
Compound of Chinese herbal medicine group mouse death rate is reduced to 12.5%, the above-mentioned pathological change of lung, intestines, kidney all be improved significantly, strongyloides intestinalis is all eliminated enteron aisle.
From the result of experiment, the method for building up for opportunistic infections and chronic wasting disease animal model of the present invention can successfully set up the animal model meeting clinical opportunistic infections and chronic wasting disease pathological process; All there is opportunistic infect and expendable cachexia in animal pattern, eye, lung, kidney, enteron aisle all occur pathological change; And modeling factor meets "Nei Jing" theory, the controllability of this modeling method tool height, reliability, repeatability and easy to use, economic; The screening therapy apparatus opportunistic infections of gained and the Chinese medicine compound prescription of chronic wasting disease have prolongation opportunistic infections and chronic wasting disease mouse life, reduce lethality, and little rathole, lung, liver, Shen Deng tissue pathologies change can be improved, and have no side effect after medication, the therapy apparatus opportunistic infections wide, easy to use, economic for a kind of efficient, low toxicity, medicine source and the Chinese medicine compound prescription of chronic wasting disease.
Claims (6)
1. the method for draft machine opportunistic infections and chronic wasting disease non-human animal model, comprises following steps:
(1) by every male non-human animal in every day 18:00 and 6-10 only, female non-human animal mates, and makes it mating,
(2) tri-time periods of next day 9:00-9:30,12:30-13:00,17:00-17:30, above-mentioned male non-human animal is swum, give feeding after each swimming terminates less than or equal to the distilled water of 4 degrees Celsius or cold water;
(3) continuous 7 days,
(4) pick out now state symptom non-human animal: illness in eye+depilation+abdominal distension diarrhoea+blood urine+opportunistic infect.
2. the process of claim 1 wherein
Described non-human animal is selected from: rat, mouse;
Described opportunistic infect comprises: streptococcus pneumonia, staphylococcus aureus, bacillus variabilis, Candida albicans, kidney mycotic infection and/or enteron aisle infection by Strongyloides stercoralis;
Described opportunistic infect is found by Pathogen test.
3. the process of claim 1 wherein step (1): by the male mouse of cleaning grade at every only 1 monthly age in every day 18:00 and 6-10 female mice mate, and make it mating;
Wherein step (2): tri-time periods of next day 9:00-9:30,12:30-13:00,17:00-17:30, makes above-mentioned male mouse swim, and gives the distilled water less than or equal to 4 degrees Celsius or cold water 3ml after each swimming terminates.
4. the Chinese medicine compound prescription being used for the treatment of opportunistic infections and chronic wasting disease of being screened by claim 1 gained non-human animal model, it is characterized in that: be made up of following raw material by weight ratio: monkshood 100 ~ 180 parts, rhizoma zingiberis 100 ~ 180 parts, 100 ~ 160 parts, Radix Glycyrrhizae.
5. Chinese medicine compound prescription according to claim 4, is characterized in that: wherein the weight proportion of each raw material is: monkshood 150 parts, rhizoma zingiberis 150 parts, 150 parts, Radix Glycyrrhizae.
6. the Chinese medicine compound prescription according to claim 4 or 5, is characterized in that: what medicine manufacture method was conveniently made is the oral liquid of whole composition or partial elements, injection, tablet, capsule or particle with it.
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| CN108969739A (en) * | 2018-08-28 | 2018-12-11 | 海南热带海洋学院 | Application of the Sini Tang in preparation prevention or treatment ulcerative colitis drug |
| BR112021009136A2 (en) * | 2018-11-14 | 2021-08-10 | Zhuhai Qiwei Bio-Technology Ltd. | screening method, method for producing a mammalian model, animal model and its method of preparation, use of an effective amount of an antibiotic, a compound and an extract |
| CN112618690A (en) * | 2021-01-06 | 2021-04-09 | 四川成康动物药业有限公司 | Injection for raising livestock at low temperature and refreshing and preparation method thereof |
| CN115088674A (en) * | 2022-06-10 | 2022-09-23 | 桂林医学院第二附属医院 | Construction method and application of echovirus 30 type wild suckling mouse model |
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