CN103430903A - Method for preparing non-human animal model with opportunistic infections and chronic wasting diseases and medicament screened by using same - Google Patents
Method for preparing non-human animal model with opportunistic infections and chronic wasting diseases and medicament screened by using same Download PDFInfo
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- CN103430903A CN103430903A CN201310409648XA CN201310409648A CN103430903A CN 103430903 A CN103430903 A CN 103430903A CN 201310409648X A CN201310409648X A CN 201310409648XA CN 201310409648 A CN201310409648 A CN 201310409648A CN 103430903 A CN103430903 A CN 103430903A
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Abstract
The invention discloses a method for establishing an animal model with opportunistic infections and chronic wasting diseases, and a traditional Chinese medicinal preparation for treating opportunistic infections and chronic wasting diseases. The traditional Chinese medicinal preparation is prepared by the following components in parts by weight: 100-180 parts of monkhood, 100-180 parts of dry ginger, and 100-160 parts of liquorice. The method has the positive effects that the method for establishing the animal model with opportunistic infections and chronic wasting diseases is found, and the model has high controllability, reliability and repeatability, and is convenient and economical to use; the model syndrome accords with the attack process of opportunistic infections and chronic wasting diseases; the model establishing factor accords with the theory of the Internal Canon of Medicine, and does not relates to causal organisms, so that the testing risks can be reduced, and the organism safety can be guaranteed; the traditional Chinese medicinal preparation obtained by screening has the characteristics of high efficiency, no toxicity, wide medicine sources and the like, is convenient to use and economical, can be used for prolonging the life of patients with opportunistic infections and chronic wasting diseases, improving the living quality of the patients and improving lesion of each tissue, and does not have toxic or side effect when a large dosage is used.
Description
Technical field
The present invention relates to a kind of non-human animal model, specifically the method for draft machine opportunistic infections and chronic wasting disease non-human animal model.The invention still further relates to the method for utilizing this model discrimination, and the therapy apparatus opportunistic infections of screening and the medicine of chronic wasting disease.
Background technology
Opportunistic infections refer to the pathogene that some pathogenicities are weak, when immune function of human body is normal, can not cause a disease, but, when immune function of human body reduces, they are taken advantage of a weak point, and invade in human body, cause various diseases.Normal flora is low at body's immunity, under the specified conditions such as position change or flora imbalance of living away from home, also can cause infection.Be found in following situation: the surface barrier effect of (1) skin and mucous membrane is damaged, as damage, burn, intubate, operation etc.(2) congenital immunity defect.Comprise: 1. humoral immune defect, without gamma globulin mass formed by blood stasis patient, repeated infection often occurs as congenital, especially bacterium infects.2. virus, tulase or fungal infection, as thymic aplasia or hypogenetic patient, very easily occur in the patient of cellular immune function defect, also easily suffers from parasitosis as Pneumocystis Carinii Infection In Rats.3. Reconstruction in Sever Combined Immunodeciency disease, patient's T cell and the immunologic function of B cell lack fully, and the birth of being everlasting is died from infection in latter 1 year.(3) secondary immunodeficiency.As patient, person in poor health and the chronic disease patient of patients with advanced cancer, application immunodepressant steroid hormone, antimetabolite, alkylating agent and antifol etc., some infects the patient as measles, infectious mononucleosis etc., often, because of immunocyte decline, easily infect.(4) acquired immunodeficiency syndrome (AIDS).AIDS is because immunity of organism is low or defect, the multiple metabolic disorder and the immune disorder that involve the many organs of whole body multisystem, consists of, and the patient often dies from the expendable cachexia after opportunistic infections and metabolism severe disorder.Human immunodeficiency virus (HIV) and non-HIV virus infections all can cause AIDS, can involve the organization systems such as eye, skin, urinary system, respiratory system, digestive system, central nervous system.The main helper lymphocyte T of invading of HIV virus, obviously lower the quantity of this cell, and infected tract is extensive, and the infected chance of patient is frequent, and patient nearly all dies from secondary infection and tumour.The HAART therapy at present, causes the treatment of acquired immunodeficiency syndrome for HIV, often adopts highly active antiretroviral therapy (HAART), although can make patient's blood plasma HIV carrying capacity and CD4
+t subset lymphocyte count is improved, but clinical symptoms appears in most of patients, worsens, even dead.Current, the generation of the acquired immunodeficiency syndrome that HIV causes is still at rapid growth, the incidence of disease is high, pathogenesis is unclear, the form of expression is various, treatment is the key character of acquired immunodeficiency syndrome without specificity, has become the hot subject of domestic and international acquired immune deficiency syndrome (AIDS) research.In addition, generation reason, the formation mechanism of the acquired immunodeficiency syndrome that non-HIV virus factor causes are also clear, and its treatment is also problem demanding prompt solution.(5) some antibiotic prolonged application, can suppress normal flora, and this is conducive to the growth of some conditioned pathogen as fungi, thereby causes opportunistic infections.(6) immunity superfluous (autoimmune disease).Infect multiple be born in autoimmune disease morbidity for many years before.The aggregate measures such as clinical normal employing immunodepressant and anti-infectives, but easily cause that the patient immune function descends, bring out opportunistic infections.(7) organ transplant is postoperative.Clinically, for suppressing the posttransplantation HVGR of organ and graft-versus-host reaction, immunosuppressive drug commonly used, but easily bring out infection.In above-mentioned situation, often show as repeated infection or severe infections disease on patient clinical, even cause death.For above-mentioned opportunistic infections, clinical major measure treatment is: (1) eliminating pathogen.Anti-infectives commonly used, as antibacterials, antifungal, antiviral agent etc., but the application of anti-infectious agent can bring in various degree the bad reaction with type, the Novel presentation that the clinical symptoms occurred in " immunologic reconstitution inflammatory syndrome (IRIS) " occurred after the anti-retroviral treatment (ART) as the suprainfection of the prolonged application of drug resistance, broad spectrum antibiotic, AIDS---body's immunity process of reconstruction worsens, vital tissue organ damage etc.(2) manage to improve the immunologic function of human body, as strengthened nutrition, defeated fresh blood or leucocyte, injection gamma globulin, thymic peptide, specific immune ribonucleic acid etc., but clinical efficacy is limited and bring certain bad reaction.Therefore, exploitation promotes body's immunity reconstruction, low toxicity or nontoxic medicine, is expected to improve the effect of therapy apparatus opportunistic infections.
Chronic wasting disease generally refers to that classes such as various malignant tumours, pulmonary tuberculosis, atrophic gastritis, severe trauma, burn, systemic loupus erythematosus, chronic purulent infection, chronic blood loss consume the body energy material excessively, cause the disease general name of human body energy negative balance.Show as apositia, atrophic gastritis, chronic colitis, chronic hepatitis, tuberculosis, tumour (especially malignant tumour), hyperthyroidism, diabetes etc.Cachexia often appears in the patient, and skeletonize, finally because of excessively weak and dead.The clinical treatment measure is: (1) for nutrition insufficiency of intake, apositia, the patient such as excessively go on a diet for a long time, often adopt the measure that increases patient's nutrition supply; (2), for patients such as atrophic gastritis, chronic colitis, chronic hepatitiss, adopt and to improve nutrient absorption, the measure such as antibiotic; (3), for patients such as tuberculosis, tumours, adopt antibiotic, antineoplaston (measures such as chemotherapy, radiotherapy, operation) and reduce the measure such as patient's nutrient consumption; (4), for patients such as hyperthyroidism, diabetes, often take to improve the treatment measure of patient's metabolic function.Above-mentioned clinical treatment measure often can cause multiple bad reaction, causes the clinical efficacy of chronic wasting disease still undesirable.Therefore, research and development are efficient, the medicine of low toxicity or nontoxic treatment chronic wasting disease, become the task of top priority.
Adopt Research of Animal Model for Study opportunistic infections and chronic wasting disease and realize than adopting human trial to be studied more easily.Utilize suitable animal opportunistic infections and chronic wasting disease model to carry out controllability, reliability, the repeatability of opportunistic infections and chronic wasting disease research tool height, can shorten flow of research, the reduce disturbance factor, reduce empirical risk simultaneously.Opportunistic infections and chronic wasting disease Research of Animal Model for Study aspect with TCM Syndrome Features belong to blank, and the science objective evaluation of sifting machine opportunistic infections and chronic wasting disease medicine and curative effect has brought obvious obstacle.
Summary of the invention
The invention reside in the method that draft machine opportunistic infections and chronic wasting disease non-human animal model are provided.
The technical scheme that realizes the object of the invention is: comprise following steps:
Comprise following steps:
(1) by every male non-human animal in every day 18:00 and 6-10 only, female non-human animal mates, and makes it mating,
(2) next day 9:00-9:30,12:30-13:00, tri-time periods of 17:00-17:30, above-mentioned male non-human animal is swum, gives feeding after each swimming finishes less than or equal to distilled water or the cold water of 4 degrees centigrade,
(3) continuous 7 days,
(4) pick out now state symptom the non-human animal: illness in eye+depilation+abdominal distension diarrhoea+blood urine+opportunistic infect.
Wherein,
Described non-human animal is selected from: rat, mouse;
Described opportunistic infect comprises: streptococcus pneumonia, staphylococcus aureus, bacillus variabilis, Candida albicans, kidney mycotic infection and/or enteron aisle infection by Strongyloides stercoralis;
Described opportunistic infect detects discovery by cause of disease.
The general using mouse is more convenient, so realize that the preferably scheme of the object of the invention is, (1): by the male mouse of clean level at every 1 monthly age in every day a 18:00 and 6-10 female mice mate, and make it mating; (2): next day 9:00-9:30,12:30-13:00, tri-time periods of 17:00-17:30, above-mentioned male mouse is swum, after finishing, each swimming gives distilled water or the cold water 3ml less than or equal to 4 degrees centigrade, (3) continuous 7 days, (4) pick out now state symptom male mouse: disease+depilation+abdominal distension diarrhoea+blood urine+opportunistic infect.
Method of the present invention is usingd appearance " illness in eye+depilation+abdominal distension diarrhoea+blood urine+opportunistic infect " as the modeling success.
The theoretical foundation that this model is set up is:
According to pathological process and the clinical symptoms of opportunistic infections and chronic wasting disease, opportunistic infections and chronic wasting disease should belong to the categories such as the traditional Chinese medical science " epidemic disease ", " consumptive disease ".Occur that opportunistic infections and chronic wasting disease external cause are mainly catching pestilential pathogens, damage body healthy tendency, internal cause is that vital essence is taken by force and caused the healthy tendency consume, and qi and blood is all empty, and with the passing of time the five internal organs are all empty, finally cause vital QI cannot conquer pathogen, and its disease is from life.We have set up the mouse model of symbol opportunistic infections and chronic wasting disease according to the "Nei Jing" theory of " consumption of normal qi causing asthenia → pathogenic domination inducing sthenia → progress of disease of struggling between the vital energy and the pathogenic factor ".
The method of draft machine opportunistic infections of the present invention and chronic wasting disease non-human animal model, its advantage is: the 1. controllability of this model tool height, reliability, repeatability and easy to use, economic; 2. this model syndrome meets the clinical onset process; 3. modeling factor meets the "Nei Jing" theory; 4. this model is set up does not need to use the causal organism infection animal, reduces empirical risk, ensures bio-safety.
Another object of the present invention is to provide the method for the medicine of screening therapy apparatus opportunistic infections and chronic wasting disease, and non-human animal model prepared according to preceding method by the method utilization, comprise the steps:
(1) the non-human animal model administration to preparing according to preceding method with alternative material, and
(2) observe, check and/or detect the opportunistic infections of this animal model and the variation of chronic wasting disease situation,
(3) picking out can mitigation symptoms, life-extending and/or reduce the material of lethality, using this medicine as therapy apparatus opportunistic infections and chronic wasting disease or as the effective ingredient of the medicine of therapy apparatus opportunistic infections and chronic wasting disease.
Wherein said alternative material comprises: cmm of simple prescription, Chinese medicine compound prescription, compound, composition.Here, Chinese medicine comprises ethnic drug, as: Tibetan medicine, anaesthetic, seedling medicine, precious jade medicine, strong medicine, Dai Nationality's medicine, Yi nationality's medicine, the She nationality, distributed over Fujian, Zhejiang, Jiangxi and Guangdong's medicine, dimension medicine.
Wherein said administration is: connect or be processed into the form of being convenient to administration by alternatives is upright, enter in this animal model by mode oral or injection.Also can take in addition any applicable administering mode.
In the present invention, the method of the medicine of screening therapy apparatus opportunistic infections and chronic wasting disease, in principle, on conventional method and the whole relevant items of all medicament research and development screening such as concrete process, experiment, detection, medicine preparation, formulation dosage, medication, all consistent with method and requirement standard, routine or that laws and regulations are stipulated.
The 3rd purpose of the present invention is to provide a kind of Chinese medicine compound prescription that is used for the treatment of opportunistic infections and chronic wasting disease.Non-human animal model prepared according to preceding method by this Chinese medicine compound prescription utilization, obtain by aforesaid screening technique.
This Chinese medicine compound prescription, made by following raw material by weight ratio: 100 ~ 180 parts of monkshoods, 100 ~ 180 parts of rhizoma zingiberis, 100 ~ 160 parts, Radix Glycyrrhizae.
The preferred weight portion proportioning of this Chinese medicine compound prescription is: 150 parts of monkshoods, 150 parts of rhizoma zingiberis, 150 parts, Radix Glycyrrhizae.
Described raw material is dry product.
Can be prepared by the method comprised the steps by this Chinese medicine compound prescription:
(1) take traditional Chinese medicine according to the bulk drug part by weight, cataclasm;
(2) add 5 ~ 9 times of water gagings of medicinal material total amount, boil rear little fire and extract 3 ~ 6 hours;
(3) filter and remove residue, obtain water extraction liquid, and gained water extraction liquid is the Chinese medicine compound prescription that is used for the treatment of opportunistic infections and chronic wasting disease.
Above-mentioned water extraction liquid, according to conventional medicine manufacture method, is made and be take its oral liquid as whole compositions or partial elements or injection, tablet, capsule, granular medicine with therapy apparatus opportunistic infections and chronic wasting disease.
The pharmacological action of this Chinese medicine compound prescription each component is:
Monkshood: hot, sweet, large heat; Poisonous.The thoughts of returning home, kidney, the spleen channel.There is recuperating depleted yang, mend fire supporing yang, by the effect of wind-cold damp pathogen.Cure mainly the yang depletion collapse, the cold weak pulse of limb, impotence, palace is cold, trusted subordinate's crymodynia, the cold of insufficiency type is vomited and diarrhoea, cold sensation of the genitalia oedema, numbness and ache of cold dampness etc." book on Chinese herbal medicine converges and says " cloud: " monkshood, return yang-energy, loose cold sensation of the genitalia.The time of all genus deficiency of yang negative electrodes, the heat symptom-complex person of lung kidney unit, clothes dead distinguished service arranged." for yin excess gram sun, loss of YANG due to profuse perspiration, vomiting and diarrhoea is fainted contrary, the cold weak pulse of limb, trusted subordinate's crymodynia, cold dysentery, the beriberi oedema, wind-cold-dampness arthralgia, impotence, palace is cold, and the cold of insufficiency type is vomited and diarrhoea, cold sensation of the genitalia oedema, deficiency of yang diseases caused by external factors, the disease of cloudy subcutaneous ulcer sore and all chronic diseaseses.And monkshood is to hypophysis---interrenal system has excitation, can also excited vagus and the tool cardiotonic.Pharmacological action: the effects such as cardiac stimulant, anti-arrhythmia, anti-shock, hemangiectasis, increase CBF, the function that strengthens interrenal system, cold resistance; And anti-inflammatory, analgesia, cold resistance, the raising effects such as tolerance to anoxic.
Rhizoma zingiberis: flavor is hot, hot in nature.Return spleen, stomach, the heart, lung channel.Have that warming spleen and stomach for dispelling cold, Hui Yang are promoted blood circulation, the effect of eliminating dampness dissolving phlegm, warming lung to reduce watery phlegm.Cure mainly coldness and pain in the epigastrium, vomit, have loose bowels, the cold weak pulse of limb, yang depletion is fainted, and contrary, cold drink is breathed with cough, numbness and ache of cold dampness.Pharmacological action: have excited guinea pig in vitro intestinal tube, excited vasomotor center and heart respiratory center, excited heart, antihistamine, antibacterial, suppress the effect such as platelet aggregation.
Radix Glycyrrhizae: distinguish the flavor of sweet, property is flat, nontoxic.There is beneficial gas bowl spares, relieving spasm to stop pain, moisten the lung and relieve the cough, the effect of purging intense heat and detonicating, coordinating the drug actions of a prescription, diuresis.Cure mainly burnout food less, thin Huang of flesh, palpitation, stomachache loose stool, the anxious pain of four limbs contraction, hysteria, cough and asthma, abscess of throat, carbuncle sore swell and ache, infant fetal toxin and medicine, food poisoning.Also for the anxious pain of weakness of the spleen and the stomach, lassitude hypodynamia, palpitation, coughing with a lot of sputum, gastral cavity abdomen and four limbs contraction, heat, drench odynuria, carbuncle sore tumefacting virus and cushion toxicity, strong.Pharmacological action: there are the functions such as anti-inflammatory, antiviral, treatment ulcer, protecting liver and detoxication, enhancing immunologic function, anti-cancer and cancer-preventing, interferon inducers and cell immunomodulator.Clinically be widely used in treating gastric and duodenal ulcer, various acute, chronic hepatitis, bronchitis, acquired immune deficiency syndrome (AIDS) and antiatherosclerosis forms.
Experiment confirms, the therapy apparatus opportunistic infections of opportunistic infections and chronic wasting disease and the Chinese medicine compound prescription of chronic wasting disease of being used for the treatment of of the present invention, there is the opportunistic infections of prolongation and chronic wasting disease mouse life, reduce lethality, and can improve the tissue pathologies changes such as opportunistic infections and the little rathole of chronic wasting disease, lung, liver, kidney, and after medication, having no side effect, is a kind of efficient, low toxicity, wide, easy to use, the economic Chinese medicine compound prescription in medicine source.
Embodiment
The Chinese medicine compound prescription preparation
Embodiment 1
Take: monkshood 150g, rhizoma zingiberis 150g, Radix Glycyrrhizae 150g.
Above-mentioned raw materials is dry product.
Then,
(1) cataclasm to 1 ~ 3mm;
(2) add water 2500g, boil rear little fire and extract 4 hours;
(3) filter and remove residue, obtain water extraction liquid 450ml.It is every milliliter contained 1 gram.
Gained water extraction liquid is the Chinese medicine compound prescription that is used for the treatment of opportunistic infections and chronic wasting disease.
Embodiment 2
Take: monkshood 100g, rhizoma zingiberis 100g, Radix Glycyrrhizae 160g.
Above-mentioned raw materials is dry product.
Then,
(1) cataclasm to 1 ~ 3mm;
(2) add water 3200g, boil rear little fire and extract 6 hours;
(3) filter and remove residue, obtain water extraction liquid 450ml.It is every milliliter contained 1 gram.
Gained water extraction liquid is the Chinese medicine compound prescription that is used for the treatment of opportunistic infections and chronic wasting disease.
Embodiment 3
Take: monkshood 180g, rhizoma zingiberis 180g, Radix Glycyrrhizae 100g.
Above-mentioned raw materials is dry product.
Then,
(1) cataclasm to 1 ~ 3mm;
(2) add water 3220g, boil rear little fire and extract 6 hours;
(3) filter and remove residue, obtain water extraction liquid 450ml.It is every milliliter contained 1 gram.
Gained water extraction liquid is the Chinese medicine compound prescription that is used for the treatment of opportunistic infections and chronic wasting disease.
Modeling and pharmacotherapy
72 of the male mouse of Kunming kind, body weight 16.9 ± 2 g, at 1 monthly age, provided credit number: GLMC20130308HL by Medical Colleges Of Guilin's zoopery center.
The male mouse of clean level at 1 monthly age is divided into to 2 groups at random: 1. Normal group (CTRL, 24): every day 9:00-9:30,12:30-13:00, tri-time periods of 17:00-17:30 are swum, after finishing, each swimming gives 28 ℃ of distilled water 3ml, continuous 7 days.2. model group (MAIDS, 48): the modeling factor comprises " sexual strain is lost smart+tired+interior raw ", every day, 18:00 first mated mating by every male mice and 6-10 female mice, carry out the swimming identical with control group daytime next day, after finishing, each swimming gives the distilled water 3ml less than or equal to 4 degrees centigrade, continuous 7 days.
After modeling success, model group is divided into not two groups for the treatment of groups and compound of Chinese herbal medicine group, 24 every group.Treatment group is not refused pharmacotherapy, every day the gavage running water, 1 milliliter every.
The compound of Chinese herbal medicine group is given the compound of Chinese herbal medicine with gained of the present invention.The Chinese medicine compound prescription raw material is totally 450 grams (monkshood 150g, rhizoma zingiberis 150g, Radix Glycyrrhizae 150g) add 2500 ml waters, decoct 450 milliliters of medicine soup, every milliliter contained 1 gram, every day gavage once, each 1 milliliter, treatment drug withdrawal after 7 days continuously, observe mouse life, and each histopathology of mouse that detects in the 28th day after administration changes.
Observe and find, 24 mouse of Normal group all do not fall ill.
And 48 mouse of model group occur in 7 days afterwards: (1) illness in eye (eye conjunctiva and ulcer of the cornea, hemorrhage, suppuration → eyes closure, blind); (2) depilation (being equivalent to patient's erythema fash); (3) digestive function not normal (serious abdominal distension, Mucous Stool); (4) the not normal deeline of metabolism such as blood urine; (5) become thin; (6) high contagiousness; (7) cause of disease detects opportunistic infects such as finding streptococcus pneumonia, staphylococcus aureus, bacillus variabilis, Candida albicans, mould, bulky stool quasi-colubriformis.The pathological change of each tissue of model group mouse is: empsyxis oedema, enteron aisle strongyloides intestinalis, kidney mycotic infection.
The lethality in treatment group morbidity mouse 7 days is not up to 87.5%.
Compound of Chinese herbal medicine group mouse death rate is reduced to 12.5%, lung, intestines, the above-mentioned pathological change of kidney all be improved significantly, strongyloides intestinalis all is eliminated enteron aisle.
From the result of experiment, the method for building up for opportunistic infections and chronic wasting disease animal model of the present invention can successfully be set up the animal model that meets clinical opportunistic infections and chronic wasting disease pathological process; Opportunistic infect and expendable cachexia all appear in animal pattern, and pathological change all appears in eye, lung, kidney, enteron aisle; And the modeling factor meets the "Nei Jing" theory, the controllability of this modeling method tool height, reliability, repeatability and easy to use, economic; The therapy apparatus opportunistic infections of screening gained and the Chinese medicine compound prescription of chronic wasting disease have the opportunistic infections of prolongation and chronic wasting disease mouse life, reduce lethality, and can improve the tissue pathologies changes such as little rathole, lung, liver, kidney, and after medication, having no side effect, is wide, easy to use, the economic therapy apparatus opportunistic infections of a kind of efficient, low toxicity, medicine source and the Chinese medicine compound prescription of chronic wasting disease.
Claims (10)
1. the method for draft machine opportunistic infections and chronic wasting disease non-human animal model comprises following steps:
(1) by every male non-human animal in every day 18:00 and 6-10 only, female non-human animal mates, and makes it mating,
(2) next day 9:00-9:30,12:30-13:00, tri-time periods of 17:00-17:30, above-mentioned male non-human animal is swum, gives feeding after each swimming finishes less than or equal to distilled water or the cold water of 4 degrees centigrade,
(3) continuous 7 days,
(4) pick out now state symptom the non-human animal: illness in eye+depilation+abdominal distension diarrhoea+blood urine+opportunistic infect.
2. the process of claim 1 wherein
Described non-human animal is selected from: rat, mouse;
Described opportunistic infect comprises: streptococcus pneumonia, staphylococcus aureus, bacillus variabilis, Candida albicans, kidney mycotic infection and/or enteron aisle infection by Strongyloides stercoralis;
Described opportunistic infect detects discovery by cause of disease.
3. the process of claim 1 wherein step (1): by the male mouse of clean level at every 1 monthly age in every day a 18:00 and 6-10 female mice mate, and make it mating;
Step (2) wherein: next day 9:00-9:30,12:30-13:00, tri-time periods of 17:00-17:30, above-mentioned male mouse is swum, after finishing, each swimming gives distilled water or the cold water 3ml less than or equal to 4 degrees centigrade.
4. the method for the medicine of screening therapy apparatus opportunistic infections and chronic wasting disease, comprise the steps:
(1) with alternative material to the administration of claim 1 gained non-human animal model, and
(2) observe, check and/or detect the variation of chronic wasting disease and/or the opportunistic infect situation of this animal model,
(3) picking out can mitigation symptoms, life-extending and/or reduce the material of lethality, using this medicine as therapy apparatus opportunistic infections and chronic wasting disease or as the effective ingredient of the medicine of therapy apparatus opportunistic infections and chronic wasting disease.
5. the method for claim 4, wherein said alternative material comprises: cmm of simple prescription, Chinese medicine compound prescription, compound, composition.
6. the method for claim 4, wherein said administration is: connect or be processed into the form of being convenient to administration by alternatives is upright, enter in this animal model by mode oral or injection.
7. the method for claim 4, wherein said opportunistic infections comprise: the opportunistic infect that the surface barrier effect of skin and mucous membrane is damaged, congenital immunity defect, secondary immunodeficiency, acquired immunodeficiency syndrome, autoimmune disease, prolonged application antibiotic and transplant operation cause;
The chronic wasting disease that described chronic wasting disease comprises and atrophic gastritis, chronic colitis, chronic hepatitis, tuberculosis, tumour, hyperthyroidism and diabetes cause
.
8. the Chinese medicine compound prescription that is used for the treatment of opportunistic infections and chronic wasting disease of being screened by claim 1 gained non-human animal model, it is characterized in that: by following raw material by weight ratio, made: 100 ~ 180 parts of monkshoods, 100 ~ 180 parts of rhizoma zingiberis, 100 ~ 160 parts, Radix Glycyrrhizae.
9. Chinese medicine compound prescription according to claim 8, it is characterized in that: wherein the weight proportion of each raw material is: 150 parts of monkshoods, 150 parts of rhizoma zingiberis, 150 parts, Radix Glycyrrhizae.
10. Chinese medicine compound prescription according to claim 8 or claim 9 is characterized in that: that according to conventional medicine manufacture method, makes take its oral liquid as whole compositions or partial elements, injection, tablet, capsule or particle.
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| CN108969739A (en) * | 2018-08-28 | 2018-12-11 | 海南热带海洋学院 | Application of the Sini Tang in preparation prevention or treatment ulcerative colitis drug |
| CN113226020A (en) * | 2018-11-14 | 2021-08-06 | 珠海岐微生物科技有限公司 | Animal models, screening methods and treatment methods for intraocular diseases or disorders |
| CN112618690A (en) * | 2021-01-06 | 2021-04-09 | 四川成康动物药业有限公司 | Injection for raising livestock at low temperature and refreshing and preparation method thereof |
| CN115088674A (en) * | 2022-06-10 | 2022-09-23 | 桂林医学院第二附属医院 | Construction method and application of echovirus 30 type wild suckling mouse model |
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| CN103430903B (en) | 2015-01-07 |
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