CN104739861A - Construction method of 'humanized' microorganism disorder depression model - Google Patents
Construction method of 'humanized' microorganism disorder depression model Download PDFInfo
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Abstract
The invention relates to a construction method of a 'humanized' microorganism disorder depression model. According to the construction method, excrement, obtained through gavage, of a depressive patient is transplanted to a germ-free mouse, and behavior assessment shows that the germ-free mouse transplanted with the excrement of the depressive patient presents typical depressed behaviors including forced swinging and prolonging of immobilization time in a tail suspension test. The model is a 'humanized' depression model for excrement transplantation of the depressive patient; carriers are provided for the researching of the action mechanism of enteric microorganisms in the occurrence of depressive disorders.
Description
Technical field
The present invention relates to animal model and build field, specifically a kind of construction method for the disorderly depression model of microorganism.
Background technology
Depression is the great mental sickness of heterogeneity that a kind of gene and environmental interaction affect.Main clinical characteristics is reduced to interest so that remarkable and lasting mental state is low.The quickening of social work rhythm of life now and the increase of competitive pressure, make the sickness rate of having a depression increase year by year.World Health Organization's data display, the annual whole world has 1.21 hundred million patients doubly to groan.The prevalence of China's depression is about 6.87%, namely about has 9,000 ten thousand people to get involved.More seriously, the patients with depression of 2/3 usually has strong suicide idea and suicide, and the patients with depression of 15-25% is finally committed suiside successfully.Therefore, the research strengthening depression mechanism is extremely urgent.
At present, depression mechanism theory mainly comprises monoamine neurotransmitter unbalance, neural generation obstacle, oxidative stress obstacle and immunoregulatory disorder etc.In these brains, abnormal theory is that the cause of disease disclosing depression provides certain theoretical foundation, but mostly can only explain the reason of some aspect.Such as, the clinical anti depressant therapy carried out based on the unbalance theory of monoamine neurotransmitter in brain only can make 40% patient symptom alleviate.As can be seen here, depression mechanism midbrain intrinsic factor is no doubt important, but external environment factor and mechanism of action thereof also can not be ignored, and is expected to become and resolves this disease new breakthrough point.
Enteric microorganism is considered to maximum, the most direct external environment of human body, plays an important role maintenance health.Enteric microorganism affects the neural biochemical and behavior phenotype of cerebral nerve of mice by " microorganism-intestinal-brain " axle.Clinical research finds, depression is disorderly with enteric microorganism, and the human body probiotic bacteria taking regulating intestinal canal microorganism can alleviate the clinical symptoms of refractory depression patient.Therefore, a kind of depressed animal model is newly invented very necessary to study the effect of enteric microorganism in depression occurs.
Summary of the invention
Object of the present invention mainly by depressive patient feces gavage is transplanted germfree mouse, and adopts the model constructed by multiple Depressive behavior evaluation methodology evaluation.
The technical solution used in the present invention is that the construction method of the disorderly depression model of " humanization " microorganism comprises:
(1) patients with depression and the n example Healthy People including the starting non-medication of n example in is as donor, and for each donor collection stage casing feces, liquid nitrogen flash freezer ,-80 DEG C of Refrigerator stores, n is positive integer.
(2) to be melted again in 4 DEG C by the feces after process in (1), then the feces of each donor takes 0.1g and is dissolved in the aseptic PBS solution of 1.5ml respectively, and concussion 5min, gets stool suspension after leaving standstill 5min.
(3) get the stool suspension mixing of the patients with depression of the starting non-medication of n example after (2) process of equivalent, get the mixed patients with depression stool suspension of 200uL and be implanted into mice in gavage mode; Equally, get the stool suspension mixing of the Healthy People of n example after (2) process of equivalent, get the mixed Healthy People stool suspension of 200uL and be implanted into mice in gavage mode.
(4) mice after gavage transplants 1 and/or 2 week carries out wilderness experiment (Open Field Test, OFT), forced swimming (Forced Swimming Test, FST) and tail-suspention test (Tail Suspension Test, TST).
At present, depression mechanism theory is numerous, mainly contains monoamine neurotransmitter unbalance, neural generation obstacle, oxidative stress obstacle and immunoregulatory disorder etc.In these brains, molecule abnormality theory is that control depression provides certain theoretical basis, but only reflects the reason in a certain respect that depression occurs.The antidepressant drug developed based on these theories also only can make some patients be benefited, and the anti depressant therapy such as carried out according to the unbalance theory of classical neurotransmitter only can make 40% patient symptom alleviate.Therefore, must traditional view be upgraded, in concern brain with depression while abnormal mechanism, pay attention to the effect of outer (external environment) factor of brain in depression occurs.Enteric microorganism is maximum, the most direct external environment of human body, and its total number is 9 times of human body cell quantity, and number gene is 150 times of human body, plays an important role maintenance health.Now there are some researches show, enteric microorganism can regulate the important physiological process of human body, with the generation of disease, develops and lapses to closely related.In view of enteric microorganism is in the important function regulating health, NIH in 2007 announces formal and starts " human microbial organizes plan ".By the animal model that the inventive method builds, Behavioral assessment is carried out to mice and finds that the germfree mouse transplanting patients with depression feces shows as typical Depressive behavior, comprise forced swimming and tail-suspention test test occurs that the dead time extends.This model is that the mechanism of action of research enteric microorganism in depression occurs provides carrier.And provide novel targets for the treatment of depression.
Accompanying drawing explanation
Fig. 1 is experiment flow figure of the present invention;
Fig. 2 is that gavage of the present invention transplants 1 Zhou Hou behavioristics result figure;
Fig. 3 is that gavage of the present invention transplants 2 Zhou Hou behavioristics result figure;
Fig. 4 is that gavage of the present invention transplants 2 Zhou Hou behavioristics result figure (containing P value);
In figure: A and B represents wilderness experimental result, C represents forced swim test result, and D represents tail-suspention test result; The mice of normal person's feces is transplanted in CON representative, and the mice of patients with depression feces is transplanted in MDD representative; The total distance of A vertical coordinate representative motion, B vertical coordinate represents centre of motion distance percentage ratio, and C, D vertical coordinate represents the dead time.
Detailed description of the invention
Specific experiment of the present invention wants summation process as follows.
1. include patients with depression and normal healthy controls diagnostic criteria in: for candidate, cure mainly Psycs's independent diagnostics of more than rank by two and adopt 17-Hamilton depressive scale to carry out scale assessment.Total score is divided into major depression more than 24, and 7-24 is divided into light modest depression, is less than 7 and is divided into without depressive symptom.
2. laboratory animal: select aseptic kunming mice in 4-6 age in week.
3. main agents: phosphate buffer (PBS).
4. key instrument: No. 12 gastric perfusion needle, agitator.
5. experimental design, flow process is see Fig. 1.
The inclusive criteria of 5.1 patients with depression and normal healthy controls and exclusion standard
Inclusive criteria: all patients with depression included in meet the diagnostic criteria of DSM-IV major depressive episode.Hamilton depression (HDRS) 17 scale score are more than or equal to 18 points.The patients with depression of First-episode, does not take any antidepressant drug.Age is greater than 18 years old, is less than 60 years old.
Exclusion standard: existing have other mental sickness medical history person with other mental sickness or the past.Merge brain organic disease and serious brain traumatism history; Merge the heart, liver, kidney diaseases, diabetes and other.Severe physical disease etc.Laboratory routine examination (routine blood test, liver function, routine urinalysis) abnormal person.Be in women's object of study of gestation, suckling, menstrual phase.Medicine and substance abuse history.
Normal healthy controls is included in and exclusion standard: impassivity mental sickness medical history, without drug dependence history or dependence history, and planless physical disease; Routine Test Lab detects Non Apparent Abnormality.
5.2 feces collection method and storage methods
Acquisition method: get stage casing feces when avoiding mixing with urine, is placed in 15mL sterile centrifugation tube.Liquid nitrogen flash freezer.
Storage method: be stored in-80 DEG C of refrigerators.
5.3 night soil-treatment and gavage implantation method
Night soil-treatment: feces melts again in 4 DEG C, and the feces of each donor takes 0.1g and is dissolved in the aseptic PBS solution of 1.5mL respectively, concussion 5min, gets suspension after leaving standstill 5min.
Gavage is transplanted: the 5 routine patients with depression stool suspension mixing of getting equivalent, gets 200uL mixed patients with depression feces PBS suspension and transplants modeling group mice in gavage mode.Equally, get 5 routine normal control stool suspension mixing of equivalent, get 200uL mixed normal control feces PBS suspension and transplant control group mice in the same fashion.
5.4 Behavioral assessment methods
Gavage is transplanted after 1 and 2 week and is carried out wilderness experiment, forced swimming and tail-suspention test.All behavioristicss record all uses video monitoring system (SMART).
Wilderness is tested: the record 5min of mice in spacious field (45cm*45cm) moves total distance, central area move distance.
Forced swimming: mice is placed in the acclimatization training that water (swimming container 15cm*20cm) carries out 15min, records the accumulative dead time in 5min after 24h.
Tail-suspention test: by the 0.5-1cm place binding of mice distance tail point, is suspended on (distance hook 5-7cm) in outstanding boot (20cm*30cm), the dead time in record 5min.
Behavioristics's data following (mice of normal person's feces is transplanted in HC representative, and the mice of patients with depression feces is transplanted in MDD representative, and P is less than 0.05 and has statistical significance)
After humanization first week
Wilderness experiment (OFT)
| Grouping | Total distance (cm) | Centre distance % |
| HC | 3.28E3±4.93E2 | 12.69±6.11 |
| MDD | 3.02E3±7.45E2 | 11.11±8.87 |
| P value | 0.255 | 0.559 |
Illustrate: after humanization transplants one week, carry out Open Field Test, acquired results is shown in Fig. 2.A: represent the total distance of the motion of two groups, does not have significant difference by between visible two groups of data.Show that the motor capacity of two groups is without significant difference.B: the centre of motion distance percentage ratio representing two groups, does not have significant difference by between visible two groups of data.Show that the anxious state of two groups is without significant difference.
Forced swimming (FST) and tail-suspention test (TST)
| Grouping | The TST dead time | TST dead time % | The FST dead time | FST dead time % |
| HC | 82.26±18.85 | 27.41±6.28 | 172.27±46.07 | 57.41±15.35 |
| MDD | 96.24±22.99 | 32.01±7.66 | 159.08±46.02 | 53.01±15.33 |
| P value | 0.72 | 0.72 | 0.417 | 0.417 |
Illustrate: after humanization transplants one week, carry out forced swimming and tail-suspention test, acquired results is shown in Fig. 2.C: represent two groups of dead times in forced swimming, does not have significant difference by between visible two groups of data.Show that the Condition of depression of two groups does not have difference.D: represent two groups of dead times in tail-suspention test, does not have significant difference by between visible two groups of data.Show that the Condition of depression of two groups does not have difference.
Second week after humanization
Wilderness is tested
| Grouping | Total distance | Centre distance % |
| MDD | 3433.91±1319.35 | 11.19±6.32 |
| HC | 3765.66±1011.53 | 15.10±5.94 |
| P value | 0.106 | 0.000 |
Illustrate: after humanization transplants two weeks, carry out Open Field Test, acquired results is shown in Fig. 3.A: represent the total distance of the motion of two groups, does not have significant difference by between visible two groups of data.Show that the motor capacity of two groups is without significant difference.B: the centre of motion distance percentage ratio representing two groups, by having significant difference between visible two groups of data and MDD group is starkly lower than HC group, illustrates that, compared with HC group, MDD group shows significant anxiety.
Forced swimming (FST) and tail-suspention test (TST)
| Grouping | The TST dead time | TST dead time % | The FST dead time | FST dead time % |
| HC | 104.33±30.54 | 34.77±10.18 | 63.02±13.37 | 21.01±4.46 |
| MDD | 114.63±28.25 | 38.20±10.18 | 88.04±23.40 | 29.35±7.80 |
| P value | 0.059 | 0.059 | 0.000 | 0.000 |
Illustrate: after humanization transplants two weeks, carry out forced swimming and tail-suspention test, acquired results is shown in Fig. 3.C: represent two groups of dead times in forced swimming, by there is significant significant difference between visible two groups of data and MDD group apparently higher than HC group, illustrate that, compared with HC group, MDD group shows significant depression.D: represent two groups of dead times in tail-suspention test, by there is significant significant difference between visible two groups of data and MDD group apparently higher than HC group, illustrate that, compared with HC group, MDD group shows significant depression.
Claims (5)
1. the construction method of the disorderly depression model of " humanization " microorganism, is characterized in that comprising:
(1) patients with depression and the n example Healthy People including the starting non-medication of n example in as donor, for each donor collection stage casing feces, liquid nitrogen flash freezer ,-80 DEG C of Refrigerator stores, n is positive integer;
(2) to be melted again in 4 DEG C by the feces after process in (1), then the feces of each donor takes 0.1g and is dissolved in the aseptic PBS solution of 1.5ml respectively, and concussion 5min, gets stool suspension after leaving standstill 5min;
(3) get the stool suspension mixing of the patients with depression of the starting non-medication of n example after (2) process of equivalent, get the mixed patients with depression stool suspension of 200uL and be implanted into mice in gavage mode; Equally, get the stool suspension mixing of the Healthy People of n example after (2) process of equivalent, get the mixed Healthy People stool suspension of 200uL and be implanted into mice in gavage mode;
(4) mice after gavage transplants 1 and/or 2 week carries out wilderness experiment, forced swimming and tail-suspention test.
2. the construction method of the disorderly depression model of " humanization " microorganism according to claim 1, is characterized in that: described mice selects aseptic kunming mice in 4-6 age in week.
3. the construction method of the disorderly depression model of " humanization " microorganism according to claim 1 or 2, is characterized in that: the experiment of described wilderness for record mice is in spacious field, the total distance of motion in 5min, the percentage ratio of central area move distance and middle distance.
4. the construction method of the disorderly depression model of " humanization " microorganism according to claim 1 or 2, is characterized in that: described forced swimming is mice is placed in the acclimatization training that water carries out 15min, records the accumulative dead time in 5min after 24h.
5. the construction method of the disorderly depression model of " humanization " microorganism according to claim 1 or 2, is characterized in that: described tail-suspention test for by the 0.5-1cm place binding of mice distance tail point, is suspended in outstanding boot, records the dead time in 5min.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107349225A (en) * | 2017-07-06 | 2017-11-17 | 广州医科大学附属第五医院 | A kind of experimental mouse model and construction method of caprophyl transplanting |
| CN110178787A (en) * | 2019-05-08 | 2019-08-30 | 河南中医药大学 | A method of self-closing disease rat model is established with caprophyl grafting |
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| CN103430903A (en) * | 2013-09-11 | 2013-12-11 | 桂林医学院 | Method for preparing non-human animal model with opportunistic infections and chronic wasting diseases and medicament screened by using same |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107349225A (en) * | 2017-07-06 | 2017-11-17 | 广州医科大学附属第五医院 | A kind of experimental mouse model and construction method of caprophyl transplanting |
| CN110178787A (en) * | 2019-05-08 | 2019-08-30 | 河南中医药大学 | A method of self-closing disease rat model is established with caprophyl grafting |
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Application publication date: 20150701 |