The dichloro quinolinic acid herbicidal agent in cigarette rice crop rotation district causes contaminated soil comprehensive repair method
Technical field
The present invention relates to the recovery technique that herbicide causes contaminated soil, the dichloro quinolinic acid herbicidal agent that especially relates to a kind of cigarette rice crop rotation district causes contaminated soil comprehensive repair method.
Background technology
The dichloro quinolinic acid chemical name is 3, the chloro-8-Quinoline Carboxylic Acid of 7-bis-, be before the selective bud by the exploitation of German BASF AG, post-emergence herbicide, be mainly used in preventing and treating barnyard grass and other grassy weeds in rice field, have that the optimum period of using is wide, consumption is few and the advantage such as herbicidal effect is good, this product has obtained registration on China paddy rice at present, and the product of the former medicine of domestic production dichloro quinolinic acid or its compound preparation has reached more than 150, becomes the herbicide product that is most widely used in current rice field.
The dichloro quinolinic acid herbicidal agent is long and strict to the time interval of plantation succession crop due to the longevity of residure.As require it to use in latter 300 days except paddy rice, can not to plant any crop, within 12 months, can not plant the crops such as tobacco.And the crop rotation of cigarette rice is the main cropping pattern of China's South Hunan, the implantation time interval deficiency of rice-cigarette 1 month.The dichloro quinolinic acid herbicidal agent is used in rice field: cigarette strain top spire blade narrows down shrinkage thickening, leaf margin to the involute shrinkage of leaf back, and it is hook-shaped that blade tip becomes, and the leaf look dark green, and tobacco leaf seriously distorts.Follow the extensive use of dichloro quinolinic acid herbicidal agent in rice field, the generation area that dichloro quinolinic acid causes the tobacco deformity phenomenon increases year by year, the extent of injury increases the weight of year by year, to the tobacco production and value amount, brings and has a strong impact on.
The restorative procedure that present dichloro quinolinic acid causes contaminated soil mainly contains: (one) tobacco-growing soil is used quick lime.The method causes to dichloro quinolinic acid the effect that contaminated soil has certain mitigation symptoms, but can not remove hazard symptoms, makes tobacco leaf recover normal.(2) efficient degrading bacteria SE08 is repaired, as: state invention public announcement of a patent application CN 103173377 A on 06 26th, 2013, a kind of MCPA,MCP,methoxone herbicide degradation bacterium SE08 and screening technique and the application of announcing, its herbicide degradation bacterium SE08, for enterobacteria (Enterobacter sp.), deposit number is CGMCCNO7016, and preservation date: 2012.12.18 is deposited in China Microbial Culture Preservation Commission's common micro-organisms center.Think and be applicable to biological prosthetic in MCPA,MCP,methoxone contaminated soil and water.Through actual experiment, use separately the method to cause the contaminated soil effect to dichloro quinolinic acid not fairly obvious.(3) use the natural plants growth regulator.As: on 07 14th, 2010, Chinese invention patent application publication No. CN 101773119 A, disclosed a kind of dichloro quinolinic acid causes agent for releasing and the application process thereof of tobacco deformity, this agent for releasing is comprised of following material: the natural plants growth regulator, the plant microecology preparation, tobacco leaf nutritional agents and synergist.Application process is: at dichloro quinolinic acid, cause the tobacco deformity early period of origination, every 1000 cigarette strains, with this agent for releasing 500g-900g, 35 times of-75 times of clear water dilutions, are evenly sprayed cigarette strain and tobacco leaf positive and negative, with the wet dew in blade face, are not dripped and be as the criterion.Through actual experiment, the method material combination is strong, and operation requirements is strict, is not suitable for the spread operation.
Summary of the invention
The dichloro quinolinic acid herbicidal agent in a kind of cigarette rice crop rotation district that purpose of the present invention provides specially for above-mentioned prior art existing problems just causes contaminated soil comprehensive repair method, the method is used chemical measure, biological control measure and physical measure to combine and is used and alleviates the effective of poisoning, makes to cause contaminated soil and recovers the long environment of normal tobacco plant student.
The objective of the invention is to be achieved through the following technical solutions:
The dichloro quinolinic acid herbicidal agent in a kind of cigarette rice crop rotation of the present invention district causes contaminated soil comprehensive repair method, is in conjunction with biomass carbon, degradation bacteria SE08 and Shi Haomei and beautiful No. 1 coupling of energy; Agent causes the contaminated soil deep ploughing to dichloro quinolinic acid herbicidal after the preceding crop results, before succession crop is transplanted, digs large cave, and upper layer of soil is turned down, and uses biomass carbon 1400-1600 kg/ha; And spray with degradation bacteria SE08 liquid; Its bacterial concentration 1.5 * 10
11Cfu/L, consumption 40-60mL/ cave; In succession crop, stretching the employing of root phase executes beautiful and can fill with root 1 time beautiful No. 1; In the prosperous long-term complete stool of succession crop and root, then even spraying is executed beautiful and can beautiful No. 12 times; Recommended density and consumption that the concentration that Shi Haomei and energy are beautiful No. 1 and consumption are manufacturer.
The above-mentioned U.S.A that executed is known agricultural chemicals, and market such as Shandong nuisanceless novel pesticide research promotion center is on sale; Be a kind of molasses fermented liquid, be mainly to utilize vegeto-animal residual body, as cane molasses, starch, beet extract and multiple beneficial biological inoculum, through modern microbial fermentation technology and highly enriched after the product made.Execute U.S. action principle 1, the nutrient of plant growth the nutrient in activating soil are provided, promoted root growth; 2, active component is the good carrier of nutrient absorption; 3, contain growth factor UGF, can excite the sprouting of root system; 4, the various active rhzomorph can directly be absorbed by root system of plant; 5, organic acid and enzyme decompose the mineral matter of indissoluble in soil to make K, P, Si, Mg, Zn etc. discharge for plant and absorb and increase fast chlorophyll with ionic species.
Above-mentioned energy is known agricultural chemicals beautiful No. 1, market is as on sale as Shaanxi Province Weinan City energy Science and Technology Ltd., a kind of anticracking extract, have activate the expansion of plurality of enzymes system and thicken nutrient delivery conduit, photosynthetic conduction fast, strengthen power conversion, accelerate the rapid absorption of plant to nutrient.Remove simultaneously resistant function between element, coordinate nutrient balance, better bring into play the characteristics of each element, the full nutrition of polynary fertilizer.
Above-mentioned biomass carbon is the organic substance that system will directly or indirectly utilize green plants photosynthesis to form; Comprise plant, animal and microorganism and excretion and metabolin except fossil fuel, with the charing production technology, carry out low-temperature carbonization, formed charcoal.
Above-mentioned degradation bacteria SE08 is enterobacteria (Enterobacter sp.), and deposit number is CGMCCNO7016, and preservation date: 2012.12.18 is deposited in China Microbial Culture Preservation Commission's common micro-organisms center.
Above-mentioned kg/ha is the meaning of kg/ha.
Above-mentioned cfu is CFU, and 1cfu/L=1/L, " cfu " is different from the method that " individual " just explains group.
The present invention is in conjunction with biomass carbon, degradation bacteria SE08 and Shi Haomei and beautiful No. 1 coupling of energy; First will treat the comprehensive repair district dry field of deep ploughing, before succession crop is transplanted, with biomass carbon, process, then dig dell topsoil is ploughed under to lower floor, be equivalent to soil-layer displacement, can be beneficial to alleviate the loss that the dichloro quinolinic acid herbicidal agent to the cigarette shoot root is.When dichloro quinolinic acid herbicidal agent poisoning is just aobvious, carry out again the use of Shi Haomei and beautiful No. 1 growth regulator of energy, induce the cigarette strain to produce a large amount of basic element of cell division and vitamin E, strengthen the photosynthesis ability, produce callus, make the plant recovery normal growth, stretching root phase employing filling root, accelerate the growth of root system, be convenient to the absorption of plant to moisture and nutrient.When the cigarette strain enters, prosperously carry out Shi Haomei when long-term and can spray growth regulator 2 times by beautiful No. 1 growth regulator complete stool, can remove preferably the hazard symptoms that dichloro quinolinic acid causes tobacco deformity.
Below that carry out in proving ground, Chenzhou City tobacco company leaf tobacco production technique center year October in August, 2011~2012.8~November in 2011, varieties of plant was excellent No. 2 late rices of Y two.Two processing: T are established in the herbicide test, and after rice transplanting, 15 d spray dichloro quinolinic acid to water, and use amount is 3 times of conventional amount used, i.e. 2250 g/ha; CK, spray the equivalent clear water, and every processing repeats for 3 times, totally 6 ,Mei residential quarter, residential quarter 100 m
2, normal water and fertilizer management.1~March in 2012 was potted plant trial test, warmhouse booth carries out in Chenzhou City tobacco company leaf tobacco production technique center, if 5 processing, be followed successively by CK1, T1, T2, T3, T4 in Table 1, observe each residential quarter tobacco situation of being injured, Taking Pictures recording, and under mensuration plant height and top, the leaf of the 4th leaf is wide, leaf is long, the calculating leaf is long, leaf is wide, the plant height inhibiting rate.
Table 1 experimental design
The impact of the different restorative procedure charcoals of table 2 on the residual vega cigarette of dichloro quinolinic acid strain growth
The present invention repairs control group through the contrast prior art and observes, from on the plant height of repairing control group, maximum leaf length, these three indexs of maximum width of blade, obviously be better than independent chemical measure control group and independent biological control measure control group, illustrate that biomass carbon, degradation bacteria SE08 and Shi Haomei and the measure of the beautiful No. 1 coupling comprehensive repair of energy be active and effective.
The specific embodiment
In conjunction with following specific embodiment, the present invention is described in further details:
Embodiment 1
The dichloro quinolinic acid herbicidal agent in a kind of cigarette rice crop rotation district of the present embodiment causes contaminated soil comprehensive repair method, be that agent causes the contaminated soil deep ploughing to dichloro quinolinic acid herbicidal after the preceding crop results, before succession crop is transplanted, dig large cave, upper layer of soil is turned down, used biomass carbon 1400 kg/ha; And spray with degradation bacteria SE08 liquid; Its bacterial concentration 1.5 * 10
11Cfu/L, consumption 40mL/ cave; In succession crop, stretching the employing of root phase executes beautiful and can fill with root 1 time beautiful No. 1; In the prosperous long-term complete stool of succession crop and root, then even spraying is executed beautiful and can beautiful No. 12 times; Recommended density and consumption that the concentration that Shi Haomei and energy are beautiful No. 1 and consumption are manufacturer.
Test card 2 results show, apply repairing method provided by the invention, the dichloro quinolinic acid herbicidal agent causes the contaminated soil reparation, the leaf of cured tobacco leaf long is significantly increased, leaf is wide and total reducing sugar extremely significantly increases, nicotine extremely significantly reduces, can efficient solution cause the tobacco deformity symptom except dichloro quinolinic acid, to the wide inhibiting rate of the leaf of lopsided tobacco leaf (%) be reduced to 9.7%, the long inhibiting rate of leaf (%) is reduced to 6.4%, plant height inhibiting rate (%) is reduced to 3.8%, makes tobacco leaf significantly recover normal botany proterties and intrinsic chemical quality.
Embodiment 2
The dichloro quinolinic acid herbicidal agent in a kind of cigarette rice crop rotation district of the present embodiment causes contaminated soil comprehensive repair method, be that agent causes the contaminated soil deep ploughing to dichloro quinolinic acid herbicidal after the preceding crop results, before succession crop is transplanted, dig large cave, upper layer of soil is turned down, used biomass carbon 1500 kg/ha; And spray with degradation bacteria SE08 liquid; Its bacterial concentration 1.5 * 10
11Cfu/L, consumption 50mL/ cave; In succession crop, stretching the employing of root phase executes beautiful and can fill with root 1 time beautiful No. 1; In the prosperous long-term complete stool of succession crop and root, then even spraying is executed beautiful and can beautiful No. 12 times; Recommended density and consumption that the concentration that Shi Haomei and energy are beautiful No. 1 and consumption are manufacturer.
Test card 2 results show, apply repairing method provided by the invention, the dichloro quinolinic acid herbicidal agent causes the contaminated soil reparation, the leaf of cured tobacco leaf long is significantly increased, leaf is wide and total reducing sugar extremely significantly increases, nicotine extremely significantly reduces, can efficient solution cause the tobacco deformity symptom except dichloro quinolinic acid, to the wide inhibiting rate of the leaf of lopsided tobacco leaf (%) be reduced to 9.2%, the long inhibiting rate of leaf (%) is reduced to 6.2%, plant height inhibiting rate (%) is reduced to 3.5%, makes tobacco leaf significantly recover normal botany proterties and intrinsic chemical quality.
Embodiment 3
The dichloro quinolinic acid herbicidal agent in a kind of cigarette rice crop rotation district of the present embodiment causes contaminated soil comprehensive repair method, be that agent causes the contaminated soil deep ploughing to dichloro quinolinic acid herbicidal after the preceding crop results, before succession crop is transplanted, dig large cave, upper layer of soil is turned down, used biomass carbon 1600 kg/ha; And spray with degradation bacteria SE08 liquid; Its bacterial concentration 1.5 * 10
11Cfu/L, consumption 60mL/ cave; In succession crop, stretching the employing of root phase executes beautiful and can fill with root 1 time beautiful No. 1; In the prosperous long-term complete stool of succession crop and root, then even spraying is executed beautiful and can beautiful No. 12 times; Recommended density and consumption that the concentration that Shi Haomei and energy are beautiful No. 1 and consumption are manufacturer.
Test card 2 results show, apply repairing method provided by the invention, the dichloro quinolinic acid herbicidal agent causes the contaminated soil reparation, the leaf of cured tobacco leaf long is significantly increased, leaf is wide and total reducing sugar extremely significantly increases, nicotine extremely significantly reduces, can efficient solution cause the tobacco deformity symptom except dichloro quinolinic acid, to the wide inhibiting rate of the leaf of lopsided tobacco leaf (%) be reduced to 9.1%, the long inhibiting rate of leaf (%) is reduced to 6.1%, plant height inhibiting rate (%) is reduced to 3.2%, makes tobacco leaf significantly recover normal botany proterties and intrinsic chemical quality.
The above is only the preferred embodiment of the present invention, but does not limit the present invention, can not assert that embodiments of the present invention are confined to these embodiment.It should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle and design, can also make some improvement or retouching, all should be considered as protection scope of the present invention.