CN103394506A - Comprehensive remediation method of 2-methyl-4-chlorophenoxyacetic acid herbicide contaminated soil in tobacco-rice rotation zones - Google Patents
Comprehensive remediation method of 2-methyl-4-chlorophenoxyacetic acid herbicide contaminated soil in tobacco-rice rotation zones Download PDFInfo
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- CN103394506A CN103394506A CN2013103486290A CN201310348629A CN103394506A CN 103394506 A CN103394506 A CN 103394506A CN 2013103486290 A CN2013103486290 A CN 2013103486290A CN 201310348629 A CN201310348629 A CN 201310348629A CN 103394506 A CN103394506 A CN 103394506A
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Abstract
The invention relates to a comprehensive remediation method of 2-methyl-4-chlorophenoxyacetic acid herbicide contaminated soil in tobacco-rice rotation zones, belonging to the remediation technology of contaminated soil. The method is characterized by deeply ploughing the 2-methyl-4-chlorophenoxyacetic acid herbicide contaminated soil after preceding crops are harvested, and digging big holes, turning down the upper layer soil and applying 1500-1900kg/ha of biomass carbon before succeeding crops are transplanted; and spraying degrading bacterium SE08 liquid, wherein the concentration of the liquid is 1.5*1011cfu/L and the dosage is 40-60mL/hole. Through combined utilization of biological measures and physical measures, the effect of relieving phytotoxicity is good, so that the tobacco botanical growth environment of the contaminated soil returns to normal.
Description
Technical field
The present invention relates to the recovery technique that herbicide causes contaminated soil, the MCPA,MCP,methoxone herbicide that especially relates to a kind of cigarette rice crop rotation district causes contaminated soil comprehensive repair method.
Background technology
Nineteen forty-one P.Pokrny reported first 2, the synthetic method of 4-D, next year P.W.Zmmerman and A.E.Hitchcock have reported 2,4-D is as the effect of plant growth regulator and herbicide, this is one of invention of great significance on the forties agricultural, so far herbicide is formally generally acknowledged, and organic epoch have been entered, subsequently 2,4-D develops rapidly, and similar kind is developed in succession, and different groups replace on phenyl ring, nineteen forty-six has been developed the MCPA,MCP,methoxone herbicide, indicates the birth of the first kind herbicide of organic synthesis-phenoxy carboxylic acid herbicides class.China used phenoxy carboxylic acid herbicides since 1956 on a large scale in rice field and vega, and wherein the MCPA,MCP,methoxone herbicide is widely used because herbicidal effect is remarkable, cheap.MCPA,MCP,methoxone belongs to the phenoxy carboxylic acid selective herbicide, has stronger interior suction conductibility, after being mainly used in seedling, cauline leaf is processed, medicament passes cuticula and cytoplasma membrane, is transmitted to finally each several part, at different parts, nucleic acid and protein is synthesized the generation Different Effects, suppress the synthetic of nucleic acid metabolism and protein on the plant top, growing point is stopped growing, and not tensible of young leaflet tablet, until photosynthesis can not normally carry out; Be transmitted to the medicament of plant bottom, make the nucleic acid of plant stem tissue and the synthetic increase of protein, promote abnormal cell division, the tip of a root expands, and loses the ability that absorbs nutrient, cause cane distortion, deformity, screen casing stops up, and bast destroys, and the organic matter transportation is obstructed, thereby destroy the normal viability of plant, finally cause plant dead.MCPA,MCP,methoxone is very sensitive to the Seedling Stage of grass, 3-4
The leaf phaseRear resistance strengthens gradually, and late tillering state is the strongest, and panicle primordium dif ferentiation stage sensitiveness rises.In temperature successful variation lower than lower than 18 ℃ the time, bad to the weeds effect of not being unearthed.Common every mu of 30-60 gram of effective ingredient consumption.Common formulations has 13%, 20% aqua, 56% wettable powder.After paddy rice was planted first quarter moon, 200～250 milliliters, every mu of use 20% aqua,, to 50 kilograms of sprayings of water, can prevent and kill off most of sedge weed and broad leaved weed.But MCPA,MCP,methoxone easily enters aquatic system, and hydrobiological toxicity be can not be ignored.MCPA,MCP,methoxone easily shifts and exists the possibility of polluted underground water at soil surface; On the other hand, since early 1970s, along with being widely used of herbicide, weeds highlight drug resistance, the resistance to the action of a drug problem that herbicide produces, occurring in nature has also produced the species of the dissimilar antiweed that gets more and more, therefore, in the elimination environment, the residual harm of herbicide is significant.
At present the MCPA,MCP,methoxone restorative procedure that causes contaminated soil mainly contains: (one) chemistry is repaired, and makes good use of bright the receipts after just aobvious to the MCPA,MCP,methoxone poisoning and sprays, and 7 days 1 time, sprays 3 times.Or with planting the power source, MCPA,MCP,methoxone is caused after the contaminated soil poisoning just shows and sprays, 7 days 1 time, spray 3 times.Or with the rape lactone, MCPA,MCP,methoxone is caused after the contaminated soil poisoning just shows and sprays, 7 days 1 time, spray 3 times.The method causes contaminated soil to MCPA,MCP,methoxone the effect of certain mitigation symptoms, but can not remove hazard symptoms, makes tobacco leaf recover normal.(2) efficient degrading bacteria SE08 is repaired, as: state invention public announcement of a patent application CN 103173377 A on 06 26th, 2013, a kind of MCPA,MCP,methoxone herbicide degradation bacterium SE08 and screening technique and the application of announcing, its herbicide degradation bacterium SE08, for enterobacteria (Enterobacter sp.), deposit number is CGMCCNO7016, and preservation date: 2012.12.18 is deposited in China Microbial Culture Preservation Commission's common micro-organisms center.Think and be applicable to biological restoration in MCPA,MCP,methoxone contaminated soil and water., through actual experiment, use separately the method effect not fairly obvious.
Summary of the invention
The MCPA,MCP,methoxone herbicide in a kind of cigarette rice crop rotation district that purpose of the present invention provides specially for above-mentioned prior art existing problems just causes contaminated soil comprehensive repair method, the method is used biological control measure and physical measure to unite and is used and alleviates the effective of poisoning, makes and causes the long environment of the contaminated soil normal tobacco plant student of recovery.
The objective of the invention is to be achieved through the following technical solutions:
The MCPA,MCP,methoxone herbicide in a kind of cigarette rice crop rotation of the present invention district causes contaminated soil comprehensive repair method, is in conjunction with biomass carbon, degradation bacteria SE08 coupling; After the preceding crop results, the MCPA,MCP,methoxone herbicide is caused the contaminated soil deep ploughing, before succession crop is transplanted, dig large cave, upper layer of soil is turned down, use biomass carbon 1500-1900 kg/ha; And with degradation bacteria SE08 liquid, spray; Its bacterial concentration 1.5 * 10
11Cfu/L, consumption 40-60mL/ cave.
Above-mentioned biomass carbon is the organic substance that system will directly or indirectly utilize green plants photosynthesis to form; Comprise plant, animal and microorganism and excretion and metabolin except fossil fuel, carry out low-temperature carbonization with the charing production technology, formed charcoal.
Above-mentioned degradation bacteria SE08 is enterobacteria (Enterobacter sp.), and deposit number is CGMCCNO7016, and preservation date: 2012.12.18 is deposited in China Microbial Culture Preservation Commission's common micro-organisms center.
Above-mentioned kg/ha is the meaning of kg/ha.
Above-mentioned cfu is CFU, and 1cfu/L=1/L, " cfu " is different from the method that " individual " just explains group.
The present invention is in conjunction with biomass carbon, degradation bacteria SE08, first will treat the comprehensive repair district dry field of deep ploughing, process with biomass carbon before succession crop is transplanted, then dig dell topsoil is ploughed under lower floor, be equivalent to soil-layer displacement, can be beneficial to alleviate the loss of MCPA,MCP,methoxone herbicide to cigarette shoot root system.
Below that carry out in proving ground, Chenzhou City tobacco company leaf tobacco production technique center year October in August, 2011～2012.8～November in 2011, varieties of plant was excellent No. 2 late rices of Y two.Two processing: T are established in the herbicide test, and after rice transplanting, 15 d spray MCPA,MCP,methoxone to water, and use amount is 3 times of conventional amount used, i.e. 2250 g/ha; CK, spray the equivalent clear water, and every processing 3 times repeats, totally 6 residential quarters, every residential quarter 100 m
2, normal water and fertilizer management.1～March in 2012 was potted plant trial test, warmhouse booth carries out in Chenzhou City tobacco company leaf tobacco production technique center, if 5 processing, be followed successively by CK1, T1, T2, T3, T4 in Table 1, observe each residential quarter tobacco situation of being injured, Taking Pictures recording, and under mensuration plant height and top, the leaf of the 4th leaf is wide, leaf is long, the calculating leaf is long, leaf is wide, the plant height inhibiting rate.
Table 1 experimental design
| Process | The rice field medicament | Restorative procedure | The vega dosing | Use period | Method of application |
| CK1 | Clear water | Clear water | 225 L/ha | 3 d after transplanting | Spray |
| T1 | MCPA,MCP,methoxone | Efficient degrading bacteria SE08 | 750 kg/ha | Before transplanting, soil treatment is 1 time, the 50mL/ cave, and after transplanting, cigarette strain rhizosphere soil is processed 1 time, the 50mL/ strain | Bacterium liquid sprays |
| T2 | MCPA,MCP,methoxone | CN 101773119 A agent for releasing | 3000g/ha | Transplanted rear about the 30th day | After 43 times of clear water dilutions, evenly spray cigarette strain and tobacco leaf positive and negative |
| T3 | MCPA,MCP,methoxone | Biomass carbon | 1800 kg/ha | Before transplanting | Puddle and bury |
| T4 | MCPA,MCP,methoxone | Biomass carbon and degradation bacteria SE08 | 1875 kg/ha | Before and after transplanting | Spray+puddle and bury |
The impact of the different restorative procedure charcoals of table 2 on the residual vega cigarette of MCPA,MCP,methoxone strain growth
The present invention repairs control group through the contrast prior art and observes, on the plant height of repairing control group, maximum leaf length, these three indexs of maximum width of blade, obviously be better than independent chemical measure control group and independent biological control measure control group, illustrate that biomass carbon and the measure of degradation bacteria SE08 coupling comprehensive repair are active and effective.
The specific embodiment
In conjunction with following specific embodiment, the present invention is described in further details:
Embodiment 1
The MCPA,MCP,methoxone herbicide in a kind of cigarette rice crop rotation district of the present embodiment causes contaminated soil comprehensive repair method, be, after the preceding crop results, the MCPA,MCP,methoxone herbicide is caused the contaminated soil deep ploughing, before succession crop is transplanted, dig large cave, upper layer of soil is turned down, used biomass carbon 1500 kg/ha; And with degradation bacteria SE08 liquid, spray; Its bacterial concentration 1.5 * 10
11Cfu/L, consumption 40mL/ cave.
Test card 2 results show, apply repairing method provided by the invention, the MCPA,MCP,methoxone herbicide causes the contaminated soil reparation, the leaf of cured tobacco leaf long is significantly increased, leaf is wide and total reducing sugar extremely significantly increases, nicotine extremely significantly reduces, can efficient solution cause the tobacco deformity symptom except MCPA,MCP,methoxone, to the wide inhibiting rate of the leaf of lopsided tobacco leaf (%) be reduced to 9.7%, the long inhibiting rate of leaf (%) is reduced to 6.4%, plant height inhibiting rate (%) is reduced to 3.8%, makes tobacco leaf significantly recover normal botany proterties and intrinsic chemical quality.
Embodiment 2
The MCPA,MCP,methoxone herbicide in a kind of cigarette rice crop rotation district of the present embodiment causes contaminated soil comprehensive repair method, be, after the preceding crop results, the MCPA,MCP,methoxone herbicide is caused the contaminated soil deep ploughing, before succession crop is transplanted, dig large cave, upper layer of soil is turned down, used biomass carbon 1900 kg/ha; And with degradation bacteria SE08 liquid, spray; Its bacterial concentration 1.5 * 10
11Cfu/L, consumption 40mL/ cave.
Test card 2 results show, apply repairing method provided by the invention, the MCPA,MCP,methoxone herbicide causes the contaminated soil reparation, the leaf of cured tobacco leaf long is significantly increased, leaf is wide and total reducing sugar extremely significantly increases, nicotine extremely significantly reduces, can efficient solution cause the tobacco deformity symptom except MCPA,MCP,methoxone, to the wide inhibiting rate of the leaf of lopsided tobacco leaf (%) be reduced to 9.2%, the long inhibiting rate of leaf (%) is reduced to 6.2%, plant height inhibiting rate (%) is reduced to 3.5%, makes tobacco leaf significantly recover normal botany proterties and intrinsic chemical quality.
Embodiment 3
The MCPA,MCP,methoxone herbicide in a kind of cigarette rice crop rotation district of the present embodiment causes contaminated soil comprehensive repair method, be, after the preceding crop results, the MCPA,MCP,methoxone herbicide is caused the contaminated soil deep ploughing, before succession crop is transplanted, dig large cave, upper layer of soil is turned down, used biomass carbon 1700 kg/ha; And with degradation bacteria SE08 liquid, spray; Its bacterial concentration 1.5 * 10
11Cfu/L, consumption 40mL/ cave.
Test card 2 results show, apply repairing method provided by the invention, the MCPA,MCP,methoxone herbicide causes the contaminated soil reparation, the leaf of cured tobacco leaf long is significantly increased, leaf is wide and total reducing sugar extremely significantly increases, nicotine extremely significantly reduces, can efficient solution cause the tobacco deformity symptom except MCPA,MCP,methoxone, to the wide inhibiting rate of the leaf of lopsided tobacco leaf (%) be reduced to 9.1%, the long inhibiting rate of leaf (%) is reduced to 6.1%, plant height inhibiting rate (%) is reduced to 3.2%, makes tobacco leaf significantly recover normal botany proterties and intrinsic chemical quality.
The above is only the preferred embodiment of the present invention, but does not limit the present invention, can not assert that embodiments of the present invention are confined to these embodiment.Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle and design, can also make some improvement or retouching, all should be considered as protection scope of the present invention.
Claims (1)
1. the MCPA,MCP,methoxone herbicide in a cigarette rice crop rotation district causes contaminated soil comprehensive repair method, it is characterized in that in conjunction with biomass carbon, degradation bacteria SE08 coupling; After the preceding crop results, the MCPA,MCP,methoxone herbicide is caused the contaminated soil deep ploughing, before succession crop is transplanted, dig large cave, upper layer of soil is turned down, use biomass carbon 1500-1900 kg/ha; And with degradation bacteria SE08 liquid, spray; Its bacterial concentration 1.5 * 10
11Cfu/L, consumption 40-60mL/ cave.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104823585A (en) * | 2015-04-15 | 2015-08-12 | 中国科学院东北地理与农业生态研究所 | Paddy field fertilizing method for in situ reduction of non-point source pollutant herbicide |
| CN106941844A (en) * | 2017-03-08 | 2017-07-14 | 安徽省农业科学院烟草研究所 | Tobacco-rice rotation integral fertilization method |
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| CN104823585A (en) * | 2015-04-15 | 2015-08-12 | 中国科学院东北地理与农业生态研究所 | Paddy field fertilizing method for in situ reduction of non-point source pollutant herbicide |
| CN106941844A (en) * | 2017-03-08 | 2017-07-14 | 安徽省农业科学院烟草研究所 | Tobacco-rice rotation integral fertilization method |
| CN106941844B (en) * | 2017-03-08 | 2019-12-06 | 安徽省农业科学院烟草研究所 | Tobacco and rice rotation integrated fertilization method |
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Application publication date: 20131120 |