The dichloro quinolinic acid herbicidal agent in Yan Dao crop rotation district causes contaminated soil comprehensive repair method
Technical field
The present invention relates to the recovery technique that herbicide causes contaminated soil, the dichloro quinolinic acid herbicidal agent that especially relates to a kind of Yan Dao crop rotation district causes contaminated soil comprehensive repair method.
Background technology
Dichloro quinolinic acid chemical name is 3, the chloro-8-Quinoline Carboxylic Acid of 7-bis-, be before the selective bud of being developed by German BASF AG, post-emergence herbicide, be mainly used in preventing and treating barnyard grass and other grassy weeds in rice field, have that the optimum period of using is wide, consumption is few and the advantage such as herbicidal effect is good, this product has obtained registration on China paddy rice at present, and the product of the former medicine of domestic production dichloro quinolinic acid or its compound preparation has reached more than 150, becomes the herbicide product being most widely used in current rice field.
Dichloro quinolinic acid herbicidal agent is because the longevity of residure is long and strict to the time interval requirement of plantation succession crop.As require it to use in latter 300 days except paddy rice, can not to plant any crop, within 12 months, can not plant the crops such as tobacco.And the crop rotation of cigarette rice is the main cropping pattern of China South Hunan, the implantation time interval deficiency of rice-cigarette 1 month.Dichloro quinolinic acid herbicidal agent is used to rear stubble tobacco in field hazard symptoms mainly in rice field: cigarette strain top spire blade narrows shrinkage thickening, leaf margin to the involute shrinkage of leaf back, and it is hook-shaped that blade tip becomes, and leaf look dark green, and tobacco leaf seriously distorts.Follow the extensive use of dichloro quinolinic acid herbicidal agent in rice field, the generation area that dichloro quinolinic acid causes tobacco deformity phenomenon increases year by year, the extent of injury increases the weight of year by year, brings and has a strong impact on to tobacco production and value amount.
The restorative procedure that current dichloro quinolinic acid causes contaminated soil mainly contains: (one) tobacco-growing soil is used quick lime.The method causes contaminated soil to dichloro quinolinic acid the effect of certain mitigation symptoms, but can not remove hazard symptoms, makes tobacco leaf recover normal.(2) efficient degrading bacteria SE08 is repaired, as: 06 Yue26Zhong state invention public announcement of a patent application CN 103173377 A in 2013, a kind of MCPA,MCP,methoxone herbicide degradation bacterium SE08 and screening technique and the application of announcing, its herbicide degradation bacterium SE08, for enterobacteria (Enterobacter sp.), deposit number is CGMCCNO7016, and preservation date: 2012.12.18 is deposited in China Microbial Culture Preservation Commission's common micro-organisms center.Think and be applicable to the biological restoration in MCPA,MCP,methoxone contaminated soil and water.Through actual experiment, use separately the method to cause contaminated soil effect to dichloro quinolinic acid not fairly obvious.(3) use natural plants growth regulator.As: on 07 14th, 2010, Chinese invention patent application publication No. CN 101773119 A, disclosed a kind of dichloro quinolinic acid causes agent for releasing and the application process thereof of tobacco deformity, this agent for releasing is made up of following material: natural plants growth regulator, plant microecology preparation, tobacco leaf nutritional agents and synergist.Application process is: cause tobacco deformity early period of origination at dichloro quinolinic acid, every this agent for releasing 500g-900g, 35 times of-75 times of clear water dilutions for 1000 cigarette strains, evenly spray cigarette strain and tobacco leaf positive and negative, do not drip and be as the criterion with the wet dew in blade face.Through actual experiment, the method material combination is strong, and operation requirements is strict, is not suitable for spread operation.
Summary of the invention
The dichloro quinolinic acid herbicidal agent in a kind of Yan Dao crop rotation district that object of the present invention provides specially for above-mentioned prior art existing problems just causes contaminated soil comprehensive repair method, the method is used chemical measure, biological control measure and physical measure to combine and uses alleviates the effective of poisoning, makes to cause contaminated soil and recovers normal tobacco plant growing environment.
The object of the invention is to be achieved through the following technical solutions:
The dichloro quinolinic acid herbicidal agent in a kind of Yan Dao crop rotation of the present invention district causes contaminated soil comprehensive repair method, is in conjunction with biomass carbon, degradation bacteria SE08 and Shi Haomei and beautiful No. 1 coupling of energy; After preceding crop results, to dichloro quinolinic acid herbicidal, agent causes contaminated soil deep ploughing, before succession crop is transplanted, digs large cave, and upper layer of soil is turned down, and uses biomass carbon 1400-1600 kg/ha; And spray with degradation bacteria SE08 liquid; Its bacterial concentration 1.5 × 10
11cfu/L, consumption 40-60mL/ cave; Stretching the employing of root phase in succession crop executes beautiful and can fill with root 1 time beautiful No. 1; In the prosperous long-term complete stool of succession crop and root, then even spraying is executed beautiful and can beautiful No. 12 times; Concentration and consumption that Shi Haomei and energy are beautiful No. 1 are recommended density and the consumption of manufacturer.
The above-mentioned U.S.A that executed is known agricultural chemicals, and novel pesticide research promotion as nuisanceless in Shandong center, market is on sale; Be a kind of molasses fermented liquid, be mainly to utilize vegeto-animal residual body, as cane molasses, starch, beet extract and multiple beneficial biological inoculum, through modern microbial fermentation technology and highly enriched after the product made.Execute U.S. action principle 1, the nutrient in nutrient the activating soil of plant growth is provided, promoted root growth; 2, active component is the good carrier of nutrient absorption; 3, contain growth factor UGF, can excite the sprouting of root system; 4, various active rhzomorph can directly be absorbed by root system of plant; 5, organic acid and enzyme decompose the mineral matter of indissoluble in soil to make K, P, Si, Mg, Zn etc. discharge for plant and absorb and increase fast chlorophyll with ionic species.
Above-mentioned energy is beautiful is for No. 1 known agricultural chemicals, market is as on sale in Weinan City energy Science and Technology Ltd. of Shaanxi Province, a kind of anticracking extract, there is the expansion of the plurality of enzymes system of activation and thicken nutrient delivery conduit, photosynthetic conduction fast, strengthen power conversion, accelerate the rapid absorption of plant to nutrient.Remove resistant function between element simultaneously, coordinate nutrient balance, better bring into play the feature of each element, the full nutrition of polynary fertilizer.
Above-mentioned biomass carbon is that system is by the organic substance that directly or indirectly utilizes green plants photosynthesis to form; Comprise plant, animal and microorganism and excretion and metabolin except fossil fuel, carry out low-temperature carbonization, the charcoal forming by charing production technology.
Above-mentioned degradation bacteria SE08 is enterobacteria (Enterobacter sp.), and deposit number is CGMCCNO7016, and preservation date: 2012.12.18 is deposited in China Microbial Culture Preservation Commission's common micro-organisms center.
Above-mentioned kg/ha is the meaning of kg/ha.
Above-mentioned cfu is CFU, and 1cfu/L=1/L, " cfu " is different from the method that " individual " just explains group.
The present invention is in conjunction with biomass carbon, degradation bacteria SE08 and Shi Haomei and beautiful No. 1 coupling of energy; First will treat the comprehensive repair district dry field of deep ploughing, before succession crop is transplanted, process with biomass carbon, then dig dell topsoil is ploughed under to lower floor, be equivalent to soil-layer displacement, can be beneficial to alleviate the loss that dichloro quinolinic acid herbicidal agent to cigarette shoot root is.In the time that dichloro quinolinic acid herbicidal agent poisoning is just aobvious, carry out again the use of Shi Haomei and beautiful No. 1 growth regulator of energy, the strain of induction cigarette produces a large amount of basic elements of cell division and vitamin E, strengthen photosynthesis ability, produce callus, make plant recovery normal growth, stretching root phase employing filling root, accelerate the growth of root system, be convenient to the absorption of plant to moisture and nutrient.Prosperously carry out Shi Haomei when long-term and can spray growth regulator 2 times by beautiful No. 1 growth regulator complete stool when cigarette strain enters, can remove preferably dichloro quinolinic acid and cause the hazard symptoms of tobacco deformity.
Below that carry out in proving ground, Chenzhou City tobacco company leaf tobacco production technique center year October in August, 2011~2012.8~November in 2011, varieties of plant was excellent No. 2 late rices of Y two.Two processing: T are established in herbicide test, and after rice transplanting, 15 d spray dichloro quinolinic acid to water, use amount is conventional amount used 3 times, i.e. 2250 g/ha; CK, sprays equivalent clear water, and every processing repeats for 3 times, totally 6 communities, every community 100 m
2, normal water and fertilizer management.1~March in 2012 is potted plant trial test, in Chenzhou City tobacco company leaf tobacco production technique center, warmhouse booth carries out, if 5 processing, be followed successively by CK1, T1, T2, T3, T4 in table 1, observe each community tobacco situation of being injured, Taking Pictures recording, and measure that the leaf of the 4th leaf under plant height and top is wide, leaf is long, calculate leaf length, Ye Kuan, plant height inhibiting rate.
Table 1 experimental design
The impact of the different restorative procedure charcoals of table 2 on the residual vega cigarette of dichloro quinolinic acid strain growth
The present invention repairs control group through contrast prior art and observes, from repairing the plant height of control group, maximum leaf length, these three indexs of maximum width of blade, obviously be better than independent chemical measure control group and independent biological control measure control group, illustrate that biomass carbon, degradation bacteria SE08 and Shi Haomei and the measure of the beautiful No. 1 coupling comprehensive repair of energy be active and effective.
Detailed description of the invention
In conjunction with following specific embodiment, the present invention is described in further details:
Embodiment 1
The dichloro quinolinic acid herbicidal agent in a kind of Yan Dao crop rotation district of the present embodiment causes contaminated soil comprehensive repair method, be that agent causes contaminated soil deep ploughing to dichloro quinolinic acid herbicidal after preceding crop results, before succession crop is transplanted, dig large cave, upper layer of soil is turned down, used biomass carbon 1400 kg/ha; And spray with degradation bacteria SE08 liquid; Its bacterial concentration 1.5 × 10
11cfu/L, consumption 40mL/ cave; Stretching the employing of root phase in succession crop executes beautiful and can fill with root 1 time beautiful No. 1; In the prosperous long-term complete stool of succession crop and root, then even spraying is executed beautiful and can beautiful No. 12 times; Concentration and consumption that Shi Haomei and energy are beautiful No. 1 are recommended density and the consumption of manufacturer.
Test card 2 results show, apply repairing method provided by the invention, dichloro quinolinic acid herbicidal agent causes contaminated soil reparation, the leaf of cured tobacco leaf long is significantly increased, leaf is wide and total reducing sugar extremely significantly increases, nicotine extremely significantly reduces, energy efficient solution is removed dichloro quinolinic acid and is caused tobacco deformity symptom, to the wide inhibiting rate of the leaf of lopsided tobacco leaf (%) be reduced to 9.7%, the long inhibiting rate of leaf (%) is reduced to 6.4%, plant height inhibiting rate (%) is reduced to 3.8%, makes tobacco leaf significantly recover normal botany proterties and intrinsic chemical quality.
Embodiment 2
The dichloro quinolinic acid herbicidal agent in a kind of Yan Dao crop rotation district of the present embodiment causes contaminated soil comprehensive repair method, be that agent causes contaminated soil deep ploughing to dichloro quinolinic acid herbicidal after preceding crop results, before succession crop is transplanted, dig large cave, upper layer of soil is turned down, used biomass carbon 1500 kg/ha; And spray with degradation bacteria SE08 liquid; Its bacterial concentration 1.5 × 10
11cfu/L, consumption 50mL/ cave; Stretching the employing of root phase in succession crop executes beautiful and can fill with root 1 time beautiful No. 1; In the prosperous long-term complete stool of succession crop and root, then even spraying is executed beautiful and can beautiful No. 12 times; Concentration and consumption that Shi Haomei and energy are beautiful No. 1 are recommended density and the consumption of manufacturer.
Test card 2 results show, apply repairing method provided by the invention, dichloro quinolinic acid herbicidal agent causes contaminated soil reparation, the leaf of cured tobacco leaf long is significantly increased, leaf is wide and total reducing sugar extremely significantly increases, nicotine extremely significantly reduces, energy efficient solution is removed dichloro quinolinic acid and is caused tobacco deformity symptom, to the wide inhibiting rate of the leaf of lopsided tobacco leaf (%) be reduced to 9.2%, the long inhibiting rate of leaf (%) is reduced to 6.2%, plant height inhibiting rate (%) is reduced to 3.5%, makes tobacco leaf significantly recover normal botany proterties and intrinsic chemical quality.
Embodiment 3
The dichloro quinolinic acid herbicidal agent in a kind of Yan Dao crop rotation district of the present embodiment causes contaminated soil comprehensive repair method, be that agent causes contaminated soil deep ploughing to dichloro quinolinic acid herbicidal after preceding crop results, before succession crop is transplanted, dig large cave, upper layer of soil is turned down, used biomass carbon 1600 kg/ha; And spray with degradation bacteria SE08 liquid; Its bacterial concentration 1.5 × 10
11cfu/L, consumption 60mL/ cave; Stretching the employing of root phase in succession crop executes beautiful and can fill with root 1 time beautiful No. 1; In the prosperous long-term complete stool of succession crop and root, then even spraying is executed beautiful and can beautiful No. 12 times; Concentration and consumption that Shi Haomei and energy are beautiful No. 1 are recommended density and the consumption of manufacturer.
Test card 2 results show, apply repairing method provided by the invention, dichloro quinolinic acid herbicidal agent causes contaminated soil reparation, the leaf of cured tobacco leaf long is significantly increased, leaf is wide and total reducing sugar extremely significantly increases, nicotine extremely significantly reduces, energy efficient solution is removed dichloro quinolinic acid and is caused tobacco deformity symptom, to the wide inhibiting rate of the leaf of lopsided tobacco leaf (%) be reduced to 9.1%, the long inhibiting rate of leaf (%) is reduced to 6.1%, plant height inhibiting rate (%) is reduced to 3.2%, makes tobacco leaf significantly recover normal botany proterties and intrinsic chemical quality.
The above is only the preferred embodiment of the present invention, but does not limit the present invention, can not assert that embodiments of the present invention are confined to these embodiment.It should be pointed out that for those skilled in the art, not departing under the prerequisite of the technology of the present invention principle and design, can also make some improvement or retouching, all should be considered as protection scope of the present invention.