CN103387469A - Biological activator granules, as well as preparation method and application thereof - Google Patents
Biological activator granules, as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention relates biological activator granules, as well as a preparation method and application thereof. At present, because temperature and humidity are difficult to control and the deterioration of colors of fruits is caused by high-concentration CO2, a fruit preservation way is difficult to popularize. The method disclosed by the invention comprises the following steps of: preparing a liquid preparation of a biological activator, drying peat and turfy soil by a roller dryer, crushing the peat and the turfy soil after drying by a crusher, performing oscillation screening after crushing, re-crushing large molecules which can not pass through a screen to small molecules till the small molecules can pass through the screen of 80 meshes, taking the small molecules as humic acid reactants, granulating by a granulating machine, then preparing humic acid granules, and spraying and adsorbing the liquid preparation of the biological activator on the humic acid granules to prepare the biological activator granules, wherein the content of the humic acid in the biological activator granules is not less than 45%, and the content of the liquid preparation of the biological activator is not less than 25%. The biological activator granules disclosed by the invention are used for base fertilizers or additional fertilizers of field crops and cash crops.
Description
technical field:
the present invention relates to agricultural biological technical field, be specifically related to a kind of bio-active agent particle and preparation method, application.
background technology:
china has been maximum in the world chemical fertilizers production state.Although cultivated area is less than 10% of whole world total amount, the applying quantity of chemical fertilizer of China approaches 1/3 of world's total amount, and usage quantity occupies the whole world the 4th.The Chinese Academy of Agricultural Sciences shows in the investigation in northern five province 20Ge counties, surpasses 50% Nitrate content in ground water in more than 800 points for investigation and exceeds standard because of excessive use nitrogenous fertilizer, healthy if this type of water source of long-term drinking will harm humans.According to statistics, China is annual because unreasonable fertilising causes the nitrogen loss of more than 1,000 ten thousand tons outside farmland, and direct economic loss reaches 30,000,000,000 yuan.And the life-time service chemical fertilizer also can cause soil property deterioration, deterioration in quality, peasant's fund input amount to strengthen year by year, Higher output is not accompanied by a higher income.Nearer next, in agriculture production, applying biological preparation progressively fertilizer for replacing chemical has become a kind of trend.Countries in the world are for all drawbacks of chemical fertilizer, developed that a series of fertility are high, cost is low, pollution-free, to person poultry harmless's biotechnological formulation.China also will progressively use the bio-feritlizer fertilizer for replacing chemical, particularly in the production of vegetables, will use as early as possible biotechnological formulation.
at present, the method for breaking soil compaction is mainly the machinery deep ploughing and uses soil improvement agent.The machinery deep ploughing is to utilize high-power agricultural machine, more than plowed soils thickness reaches 60cm, and the soil of loose sclerosis, the reconstruct crumb structure, increase soil fertility, water-retaining effect, this method is pollution-free, does not destroy profitable strain.Shortcoming is that the soil amount of stirring is large, easily forms uneven ridges, and impact allows planeness, and labour intensity is large, and cost is high.Use effectively improved soil nodule structure of soil improvement agent, play the effect of ventilation, water-retaining effect.Soil improvement agent comprises synthetic modifying agent and natural modifying agent.Polyacrylamide, the synthetic modifying agent costs such as asphalt emulsion are high, and the time length is short, and ecotope is had to disadvantageous effect.Natural modifying agent has same improved effect as saccharan, humic acid, peat, but cost is relatively low, and the greenization environmental protection has obvious advantage.
at present and even from now on, the raising of crop yield, in the constant situation of per unit area yield, will only have by enlarging cultivated area or increasing multiple cropping and will realize; In the constant situation of cultivated area, only have the approach of the crop yield by improving unit surface to realize.
the quality of crop product refers to the quality of product, reaches the fitness of certain requirement of people, is directly connected to the economic worth of product product.
the fruit freshness preserving mode mainly contains preservation by low temperature method, controlled atmosphere method, chemical fresh-keeping method etc. at present, but because temperature and humidity is difficult to control, high concentration CO
2
cause the bad change of its color and cost is high, mycocide relates to food-safety problem, make these methods be difficult to promote.Therefore, research and development are controlled fruit without residual hazard, free of contamination New Biological Preservatives and are adopted the rear rotten focus that becomes research and development.
summary of the invention:
the purpose of this invention is to provide a kind of bio-active agent particle and preparation method, application, the liquid preparation and the biologically active substance that utilize gel-shaped series bacillus (Paenibacillus mucilaginosus preserving number CGMCC1.3714) fermentation to expand after numerous carry out the composite agricultural biological activator product obtained.
above-mentioned purpose realizes by following technical scheme:
a kind of preparation method of bio-active agent particle, the method comprises the steps: to prepare the liquid preparation of bio-active agent, by peat and turfy soil through the roller dryer drying, dried described peat and described turfy soil are pulverized with pulverizer, shaken sub-sieve after pulverizing, the macromole that can not sieve is crushed to small molecules again until by sieve mesh 80 orders, as the humic acids reactant, after granulator granulation, make the humic acids particle, the spraying of the liquid preparation of described bio-active agent is adsorbed on described humic acids particle, make the bio-active agent particle, the content of the humic acid in described bio-active agent particle>=45%, the liquid preparation of described bio-active agent>=25%.
the preparation method of described bio-active agent, the described liquid preparation for preparing bio-active agent is to add substratum in fermentor tank, by glucose, W-Gum, yeast powder, bean cake powder, manganous sulfate, dissolution of calcium carbonate is in the tap water purified, the weight fraction of described glucose is 1.8%, described W-Gum weight fraction is 1.7%, described yeast powder weight fraction is 3.0%, described bean cake powder weight fraction is 3.2%, described manganous sulfate weight fraction is 0.05%, described weight of calcium carbonate mark is 0.3%, 121 ℃ of sterilizings 30 minutes, access gel-shaped series bacillus, the preserving number CGMCC1.3714 of described gel-shaped series bacillus, described gel-shaped series bacillus is purchased from the common micro-organisms center C GMCC of China Committee for Culture Collection of Microorganisms, be numbered CGMCC NO.1.3714, address is Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, the inoculum size of described gel-shaped series bacillus is 10%, start fermentation, during fermentation, culture temperature is 28-30 ℃, tank pressure 0.05mPa/cm
2
, stirring velocity 220rpm/ minute, air flow 1:1, initial pH7.0-7.2 of fermenting, microscopy under the sampling microscope, produce spore 90% and complete with top fermentation, obtains the liquid preparation of described bio-active agent.
a kind of bio-active agent particle that utilizes above-mentioned method to make, the living bacteria count of the gel-shaped series bacillus of described bio-active agent particle>=0.2 hundred million/gram, miscellaneous bacteria rate≤100/gram, the content of humic acids>=40%.
a kind of application of the bio-active agent particle that utilizes above-mentioned method to make, described bio-active agent particle mix use with composite fertilizer or chemical fertilizer, as base manure or topdress, reach improvement soil regime, increase soil fertility, improve crop yield, improve crop quality, improve the purpose of crop anti-adversity.
the application of the described bio-active agent particle that utilizes above-mentioned method to make, described bio-active agent particle and composite fertilizer or chemical fertilizer are used in conjunction with, and are used as base manure or topdress, and for the plantation of corn, soybean, beet, improve crop yield; Plantation for fruit tree, improve crop quality.
beneficial effect:
1. the present invention adopts gel-shaped series bacillus (Paenibacillus mucilaginosus preserving number CGMCC1.3714).This is had to the special effective function strain fermentation and expand the biologically active substance extracted in the crude substance such as liquid preparation after numerous and humic acid and be combined with each other by Particular craft, produce high-quality and efficient bio-active agent product.Major technique and performance index reach the You effect Jun Shuo>=0.2 hundred million/gram of living, miscellaneous bacteria Shuai<=100/gram, You Ji Zhi>=40%.A kind of bio-active agent product is carried out NY 884-2012 standard.
2. the present invention gropes by biological test and production technique, through the every detection of above-mentioned bio-active agent identify, the chemical examination pointer all reaches or be better than national existing industry standard, major technique and performance index reach You effect Jun Shuo alive>=0.2 hundred million/gram, miscellaneous bacteria Shuai<=100/gram, You Ji Zhi>=40%, the quality pointer is reliable and stable.In product effectively the technical indicator such as viable bacteria all reach or be better than national standard.
3. the present invention can increase soil fertility for improveing soil regime, improves crop yield, improves crop quality, can improve again the resistance of crop.It is suitable for various edatopes and weather condition, can mix use with composite fertilizer or chemical fertilizer, is used as base manure or topdresses.Farm crop are used this product, and per unit area yield improves output 10%-30%; The crop ripening stage shortens 7-15 days, but and the freshness date of extending fruit.A kind of every chemical examination pointer of bio-active agent all reaches or is better than national existing industry standard (NY884 2012), major technique and performance index reach You effect Jun Shuo alive>=0.2 hundred million/gram, miscellaneous bacteria Shuai<=100/gram, You Ji Zhi>=40%, the quality pointer is reliable and stable.
4. the present invention is in order to reach the purpose of increasing soil fertility, and the present invention uses described bio-active agent to mix use with composite fertilizer or chemical fertilizer, is used as base manure or topdresses.
5. the present invention is in order to reach the purpose that improves crop yield, and the present invention uses described bio-active agent to mix use with composite fertilizer or chemical fertilizer, is used as base manure or topdresses.
6. the present invention is in order to reach the purpose that improves crop quality, and the present invention uses described bio-active agent to mix use with composite fertilizer or chemical fertilizer, is used as base manure or topdresses.
7. the present invention is in order to reach the purpose that improves crop anti-adversity, and the present invention uses described bio-active agent and composite fertilizer or chemical fertilizer to be used in conjunction with, and is used as base manure or topdresses.
the accompanying drawing explanation:
accompanying drawing 1 is the process flow sheet of method of the present invention.
accompanying drawing 2 is field of the present invention distribution plans.
embodiment:
embodiment 1:
a kind of preparation method of bio-active agent particle, the method comprises the steps: to prepare the liquid preparation of bio-active agent, by peat and turfy soil through the roller dryer drying, dried described peat and described turfy soil are pulverized with pulverizer, shaken sub-sieve after pulverizing, the macromole that can not sieve is crushed to small molecules again until by sieve mesh 80 orders, as the humic acids reactant, after granulator granulation, make the humic acids particle, the spraying of the liquid preparation of described bio-active agent is adsorbed on described humic acids particle, make the bio-active agent particle, the content of the humic acid in described bio-active agent particle>=45%, the liquid preparation of described bio-active agent>=25%,
the described liquid preparation for preparing bio-active agent is to add substratum in fermentor tank, by glucose, W-Gum, yeast powder, bean cake powder, manganous sulfate, dissolution of calcium carbonate is in the tap water purified, the weight fraction of described glucose is 1.8%, described W-Gum weight fraction is 1.7%, described yeast powder weight fraction is 3.0%, described bean cake powder weight fraction is 3.2%, described manganous sulfate weight fraction is 0.05%, described weight of calcium carbonate mark is 0.3%, 121 ℃ of sterilizings 30 minutes, access gel-shaped series bacillus, described gel-shaped series bacillus (Paenibacillus mucilaginosus preserving number CGMCC1.3714), described gel-shaped series bacillus (Paenibacillus mucilaginosus) is purchased from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), be numbered CGMCC NO.1.3714, address is Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica,
the inoculum size of described gel-shaped series bacillus is 10%, starts fermentation, and during fermentation, culture temperature is 28-30 ℃, tank pressure 0.05mPa/cm
2
, stirring velocity 220rpm/ minute, air flow 1:1, initial pH7.0-7.2 of fermenting, microscopy under the sampling microscope, produce spore 90% and complete with top fermentation, obtains the liquid preparation of described bio-active agent;
technological process:
1: turfy soil---drying---pulverizing---sub-sieve---granulation
2: purifying agaric---fermentation culture---tunning purifying
3: above-mentioned gained 1 and 2 is composite, and------packing---quality inspection---is dispatched from the factory to make finished product.
embodiment 2:
a kind of bio-active agent particle that utilizes method to make, the living bacteria count of the gel-shaped series bacillus of described bio-active agent>=0.2 hundred million/gram, miscellaneous bacteria rate≤100/gram, the content of humic acids>=40%.
according to requirement and the detection method of " complex microorganism material " industry standard (NY 884-2012), product is tested, living bacteria count in the standard-required qualified product>=2 * 10
7
individual/gram, miscellaneous bacteria rate≤100/gram, the pH value is 5.5-8.5, organic>=40%.Through a large amount of evidences, a kind of bio-active agent of the present invention can reach following technical indicator, and heavy metal content does not surpass the innoxious index of Ministry of Agriculture's bio-feritlizer industry standard, as shown in table 1:
the technical indicator that table 1 adopts different ingredients to produce a kind of bio-active agent
above-mentioned/gram refers to the number that contains microorganism in every gram solid; % refers to the mass fraction that adds and contain target compound in the solid of every hundred parts of quality.
embodiment 3:
the bio-active agent particle that a kind of embodiment of utilization 1-2 method makes, test examination example 1: a kind of bio-active agent improvement soil regime:
1. prepared a kind of bio-active agent particle according to embodiment 1-2.
2. experimental plot: corn experimental plot, Fujin City agricultural science and technology demonstration area
3. test period: in April, 2008---in May, 2008
4. using method: a kind of bio-active agent with as base manure and topdress, take and use distilled water as control group.
5. sampling, measuring method are with reference to State Standard of the People's Republic of China's standard of soil environment quality (GB15618-1995).Measure respectively control group and soil organic matter content, water content, the unit weight of using experimental group of the present invention.
impact on soil organic matter content, water content, unit weight is as shown in table 2:
the impact of table 2 the present invention on soil organic matter content, water content, unit weight
as shown in Table 2, using soil organic matter content after the present invention, water content, unit weight all is significantly increased.Result shows that the present invention can effectively improve soil particle degree, breaks soil compaction, increases soil fertility and water content.There is obvious reclamation result.
embodiment 4:
the bio-active agent particle that a kind of embodiment of utilization 1-2 method makes, test examination example 2: a kind of bio-active agent is increase soil fertility:
1. prepared a kind of bio-active agent particle according to embodiment.
2. experimental plot: corn experimental plot, Fujin City agricultural science and technology demonstration area
3. test period: in April, 2008---in May, 2008
4. using method: a kind of bio-active agent with as base manure and topdress.
5. sampling, measuring method are measured respectively the soil index with reference to the State Standard of the People's Republic of China.
the present invention is as shown in table 3 on the impact of soil fertility:
the impact of table 3 the present invention on soil fertility
after using the present invention as shown in Table 3, soil fertility is significantly increased, and result shows to use each index of soil of the present invention and reaches and exceed national standard, has obvious fertility and promotes effect.
embodiment 5:
the bio-active agent particle that a kind of embodiment of utilization 1-2 method makes, test examination example 2: a kind of bio-active agent improves crop yield:
one, test materials
1. for examination fertilizer: according to embodiment 1-2, prepared a kind of bio-active agent.
2. contrast fertilizer: 46% English the United Nations General Assembly beans special fertilizer (13-20-13) is provided by Fujin City agricultural technology extension center.
3. trial crops: soybean, kind is for cultivating rich 16
two, test site and time
test site: Fujin City agricultural science and technology demonstration area
test period: in April, 2008---in September, 2008
three, test method
be experimental field that smolmitza, middle fertility, physical features are smooth, be applicable to manufacturing soybean.Test is made as 3 treatment group and 1 control group:
10 kg/acres of 17 kilograms+a kind of bio-active agents for the treatment of group 1:46% English the United Nations General Assembly beans special fertilizer (13-20-13)
7.5 kg/acres of 17 kilograms+a kind of bio-active agents for the treatment of group 2:46% English the United Nations General Assembly beans special fertilizer (13-20-13)
5 kg/acres of 17 kilograms+a kind of bio-active agents for the treatment of group 3:46% English the United Nations General Assembly beans special fertilizer (13-20-13)
17 kg/acres for the treatment of group 4:46% English the United Nations General Assembly beans special fertilizers (13-20-13)
adopt the great Qu contrast, do not establish repetition, each great Qu 12 ridge, long 80 meters, 624 squares of row spacing 0.65 meter ， great district areas.Front stubble soybean, the autumn subsoiling, the autumn ridging, the autumn suppression reaches state to be broadcast, and the soybean varieties of participating in the experiment is for cultivating rich 16, sowing on May 9, field three shovel three, optimum period is used base manure: hectare was used and was surveyed soil formula soybean special use 750 grams that topdress, 5 kilograms, 2.5 kilograms+urea of hectare application of phosphoric acid potassium dihydrogen on June 25 June 12, the insect pests crop smothering, take the large grass in clean field in time.
four, results and analysis, as shown in table 4,5,6:
climatological data when table 4 bio-active agent affects crop yield
table 5 questionnaire breeding time
| Process | Sowing time | Seeding stage | Early flowering season | Full-bloom stage | The drum grain phase | Ripening stage |
| Process 1 | 5.9 | 5.22 | 6.26 | 7.25 | 8.23 | 9.19 |
| Process 2 | 5.9 | 5.22 | 6.24 | 7.22 | 8.21 | 9.16 |
| Process 3 | 5.9 | 5.22 | 6.24 | 7.23 | 8.22 | 9.17 |
| Process 4 | 5.9 | 5.23 | 6.27 | 7.25 | 8.24 | 9.20 |
the analysis of table 6 yield result
, test-results shows, a kind of bio-active agent can make the crop ripening stage shorten 7-15 days, increases output 15%-30%.
embodiment 6:
the bio-active agent particle that a kind of embodiment of utilization 1-2 method makes, test examination example 3:
one, test materials
1. for examination fertilizer: according to example 1---example 2 has prepared a kind of bio-active agent.
2. contrast fertilizer: 64% diammonium phosphate (U.S.'s product) Fujin City agricultural technology extension center provides.
46% urea (grand celebration product) Fujin City agricultural technology extension center provides.
50% granular potassium sulfate (Shandong product) Fujin City agricultural technology extension center provides.
3. trial crops: corn, kind is for pacifying beautiful 15
two, test site and time
test site: Fujin City agricultural science and technology demonstration area
test period: in April, 2008---in September, 2008
three, test method
be experimental field that smolmitza, middle fertility, physical features are smooth, be applicable to manufacturing soybean.Test is made as 3 treatment group and 1 control group:
process 15 kg/acres of 5 kilograms+a kind of bio-active agents of 1:64% diammonium phosphate 13.3 kilograms+46% urea 6.7 kilograms+50% vitriolate of tartar;
process 10 kg/acres of 5 kilograms+a kind of bio-active agents of 2:64% diammonium phosphate 13.3 kilograms+46% urea 6.7 kilograms+50% vitriolate of tartar;
process 5 kg/acres of 5 kilograms+a kind of bio-active agents of 3:64% diammonium phosphate 13.3 kilograms+46% urea 6.7 kilograms+50% vitriolate of tartar;
5 kg/acres of control group 4:64% diammonium phosphate 13.3 kilograms+46% urea 6.7 kilograms+50% vitriolate of tartar;
adopt the community ratio method, random processing, repeat for three times, each 4 ridge, community, long 10 meters, 26 squares of row spacing 0.65 meter ， community areas.Front stubble soybean, the autumn subsoiling, the autumn ridging, the autumn suppression reaches state to be broadcast, and the corn variety of participating in the experiment is peaceful jade 15, sowing on May 10, field three shovel three, used and once survey the native corn Special basal fertilizer of filling a prescription on June 18, and the jointing stage chases after 150 kilograms, urea.The field distribution plan, as shown in Figure 2.
four, results and analysis, as shown in table 7,8,9,10,11:
climatological data when table 7 bio-active agent of the present invention affects crop yield
table 8 questionnaire breeding time
| Process | Sowing time | Seeding stage | Jointing stage | Reel off raw silk from cocoons the phase | Ripening stage |
| Process 1 | 5.10 | 5.25 | 6.29 | 7.27 | 9.15 |
| Process 2 | 5.10 | 5.25 | 6.29 | 7.27 | 9.15 |
| Process 3 | 5.10 | 5.25 | 6.30 | 7.28 | 9.17 |
| Process 4 | 5.10 | 5.25 | 7.2 | 8.1 | 9.19 |
the analysis of table 9 yield result
table 10 cell production table 354
| Community | 1 | 2 | 3 | Ti | Mean number |
| Process 1 | 21.87 | 22.35 | 20.9 | 65.12 | 21.71 |
| Process 2 | 21.97 | 21.58 | 20.67 | 64.22 | 21.41 |
| Process 3 | 18.82 | 20.03 | 20.08 | 58.93 | 19.64 |
| Process 4 | 18.85 | 16.31 | 20.54 | 55.7 | 18.57 |
| Xt | 81.51 | 80.27 | 82.19 | 243.97 | ? |
table 11 analysis of variance table
| Source of variation | DF | SS | MS | F | F 0.05 | F 0.01 |
| Between district's group | 2 | 0.48512 | 0.24256 | 0.123 | 5.14 | ? |
| Between processing | 3 | 19.90583 | 6.6353 | 3.371 | 4.76 | ? |
| Error | 6 | 11.581142 | 1.9686 | ? | ? | ? |
| Total variation | 11 | 31.972092 | ? | ? | ? | ? |
test-results shows, a kind of bio-active agent can make the crop ripening stage shorten 7-15 days, increases output 6%-17%.
embodiment 7:
the bio-active agent particle that a kind of embodiment of utilization 1-2 method makes, experimental program:
1, for showing material: a kind of bio-active agent that Heilongjiang Province's rainbow profit biotechnology company limited produces.
2, demonstration methods: adopt the great Qu ratio method, do not establish repetition, 10 mu for the treatment of zone areas, 5 mu of check plot areas.
3, demonstration kind: soybean adopts kind " black agriculture 48 ", and the corn planting kind be " first jade 335 " (the county institute of agricultural sciences), middle peasant 68(Long Miao town), high yield king (ruddiness township); The beet varieties of plant is KWS3418.
4, test site: be located to corn and soybean demonstration Yan Long village, beam man of virtue and ability Bin Jia ，Long Miao town, rich rural area, ruddiness township, Bayan County Zheng Zhen Duo Jia， county Zhang Xibo family of the institute of agricultural sciences; Be located to the beet demonstration Yong Chang village, Song Hua River Ma Jinfu family.
5, test period: in April, 2008---in September, 2008
demonstration is layouted and processing list, as shown in table 12;
table 12 demonstration is layouted and processing list
soybean thing marquis phase questionnaire, as shown in table 13:
table 13 soybean phenological period questionnaire
soybean is surveyed and produces table, as shown in table 14:
table 14 soybean side is produced table
corn phenological period questionnaire, as shown in Table 15:
Table 15 corn phenological period questionnaire
corn phenological period questionnaire, shown in table 16:
table 16 corn is surveyed and is produced table
the beet questionnaire, shown in table 17:
table 17 beet questionnaire
two, agronomic measures
the agronomic measures such as each crop sowing, field management are same as local land for growing field crops.
three, demonstration result
can find out through the demonstration of a year, apply the not impact on the phenological period of soybean, corn and beet of a kind of bio-active agent.(in Table 13,15,16) are from output:
1, soybean: ruddiness township mu volume increase 16.5kg, increase production 11.5%.
long Miao town mu volume increase 12.8kg, increase production 9.3%.
the mu volume increase 15.0kg of the county institute of agricultural sciences, increase production 10.1%.
three the average mu volume increase of point 14.8 ㎏, increase production 10.3%.
(in Table 14)
2, corn: ruddiness township mu volume increase 75.2kg, increase production 11.4%.
long Miao town mu volume increase 77.5kg, increase production 15.1%.
the mu volume increase 50.1kg of the county institute of agricultural sciences, increase production 7.1%.
three the average mu volume increase of point 67.6 ㎏, increase production 11.2%.
(in Table 16)
3, beet: mu volume increase 552kg, increase production 22.1%.
four, conclusion
demonstration by 1 year sees, a kind of bio-active agent all has certain production-increasing function to three crops for examination, and wherein on the cash crop beet, performance is comparatively obvious, and amplitude reaches 22.1%.And study thing without any negative interaction and detrimentally affect to supplying.
embodiment 7:
the bio-active agent particle that a kind of embodiment of utilization 1-2 method makes, experimental program:
1. prepared a kind of bio-active agent according to embodiment 1-2.
2. experimental plot: experimental plot, Fujin City agricultural science and technology demonstration area
3. test period: in April, 2008---in May, 2008
4. using method: a kind of bio-active agent with as base manure and topdress, take and spray distilled water as control group.
test-results shows, a kind of bio-active agent can make the anti-low temperature of crop, drought-resistant, resistant to lodging.
embodiment 8:
the bio-active agent particle that a kind of embodiment of utilization 1-2 method makes, experimental program: bio-active agent improves crop quality:
1. prepared a kind of bio-active agent according to example 1-example 2.
2. a bio-active agent, use once before blooming of fruit trees or after blooming, and in the phase of bearing fruit, sprays for the second time.Take and use distilled water as control group.
3. experimental plot is fruit tree garden, somewhere, Harbin.
test period in May, 2008---in September, 2008.
subjects: kyoto grape.
the impact of bio-active agent of the present invention on crop quality, as shown in the table:
the impact of table 18 bio-active agent of the present invention on crop quality
| ? | Shape | Size | Flavour | Color and luster |
| Treatment group | Rule is full | Diameter 0.9-1.2cm | Fragrant and sweet | Bright, dark purple |
| Control group 1 | Not of uniform size | Diameter 0.7-1.1cm | Sweet taste | Purple, spottiness |
| Control group 2 | Not of uniform size | Diameter 0.7-1.0cm | Sweet taste, slightly acid | Slightly red, bloom is arranged |
the mean value that in table 7, the data of shape, flavour grade and color and luster are popular evaluation sample survey.
test-results shows, uses ensuing crop quality of the present invention and has greatly improved.
embodiment 9:
the application of the bio-active agent particle that a kind of embodiment of utilization 1-2 method makes, described bio-active agent particle mix use with composite fertilizer or chemical fertilizer, as base manure and topdressing, reach improvement soil regime, increase soil fertility, improve crop yield, improve crop quality, improve the purpose of crop anti-adversity.
embodiment 10:
the application of the bio-active agent particle that the described embodiment of utilization 1-2 method makes, described bio-active agent particle and composite fertilizer or chemical fertilizer are used in conjunction with, and are used as base manure and topdress, and for the plantation of corn, soybean, beet, improve crop yield; Plantation for fruit tree, improve crop quality.
Claims (5)
1. the preparation method of a bio-active agent particle, it is characterized in that: the method comprises the steps: to prepare the liquid preparation of bio-active agent, by peat and turfy soil through the roller dryer drying, dried described peat and described turfy soil are pulverized with pulverizer, shaken sub-sieve after pulverizing, the macromole that can not sieve is crushed to small molecules again until by sieve mesh 80 orders, as the humic acids reactant, after granulator granulation, make the humic acids particle, the spraying of the liquid preparation of described bio-active agent is adsorbed on described humic acids particle, make the bio-active agent particle, the content of the humic acid in described bio-active agent particle >=45%, the liquid preparation of described bio-active agent >=25%.
2. the preparation method of bio-active agent according to claim 1, it is characterized in that: the described liquid preparation for preparing bio-active agent is to add substratum in fermentor tank, by glucose, W-Gum, yeast powder, bean cake powder, manganous sulfate, dissolution of calcium carbonate is in the tap water purified, the weight fraction of described glucose is 1.8%, described W-Gum weight fraction is 1.7%, described yeast powder weight fraction is 3.0%, described bean cake powder weight fraction is 3.2%, described manganous sulfate weight fraction is 0.05%, described weight of calcium carbonate mark is 0.3%, 121 ℃ of sterilizings 30 minutes, access gel-shaped series bacillus, described gel-shaped series bacillus is purchased from the common micro-organisms center C GMCC of China Committee for Culture Collection of Microorganisms, be numbered CGMCC NO.1.3714, the inoculum size of described gel-shaped series bacillus is 10%, start fermentation, during fermentation, culture temperature is 28-30 ℃, tank pressure 0.05mPa/cm
2, stirring velocity 220rpm/ minute, air flow 1:1, initial pH7.0-7.2 of fermenting, microscopy under the sampling microscope, produce spore 90% and complete with top fermentation, obtains the liquid preparation of described bio-active agent.
3. a bio-active agent particle that utilizes claim 1-2 method to make is characterized in that: the living bacteria count of the gel-shaped series bacillus of described bio-active agent particle >=0.2 hundred million/gram, miscellaneous bacteria rate≤100/gram, the content of humic acids >=40%.
4. the application of a bio-active agent particle that utilizes claim 1-2 method to make, it is characterized in that: described bio-active agent particle mix use with composite fertilizer or chemical fertilizer, as base manure or topdress, reach improvement soil regime, increase soil fertility, improve crop yield, improve crop quality, improve the purpose of crop anti-adversity.
5. the application of the bio-active agent particle that utilizes claim 1-2 method to make according to claim 4, it is characterized in that: described bio-active agent particle and composite fertilizer or chemical fertilizer are used in conjunction with, be used as base manure or topdress, for the plantation of corn, soybean, beet, improving crop yield; Plantation for fruit tree, improve crop quality.
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| CN2013103522117A CN103387469A (en) | 2013-08-14 | 2013-08-14 | Biological activator granules, as well as preparation method and application thereof |
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| CN105152752A (en) * | 2015-08-07 | 2015-12-16 | 沃达农业科技股份有限公司 | Humic acid containing microbial ecological fertilizer as well as preparation and application methods thereof |
| CN106748347A (en) * | 2017-03-30 | 2017-05-31 | 山西广宇通科技股份有限公司 | A kind of method that utilization fluid bed prepares humic acid complex micro organism fungicide |
| CN110229039A (en) * | 2019-06-14 | 2019-09-13 | 南京农业大学 | The method of humic acid and the biological organic fertilizer of preparation in a kind of bioactivation wood peat |
| CN111187744A (en) * | 2020-03-16 | 2020-05-22 | 厦门大学 | High-density industrial fermentation medium for stratospheric bacillus and fermentation method thereof |
| CN112341275A (en) * | 2020-11-11 | 2021-02-09 | 宁夏陈敷生态科技有限公司 | Good carrier capable of preserving agricultural microbial agent for long time |
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Inventor after: Zhang Binshan Inventor after: Zhang Xiaofeng Inventor before: Zhang Binshan |
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Application publication date: 20131113 |