CN103342609A - Straw thoroughly-decomposing microbial inoculum capable of being preserved for long time under normal temperature condition - Google Patents
Straw thoroughly-decomposing microbial inoculum capable of being preserved for long time under normal temperature condition Download PDFInfo
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- CN103342609A CN103342609A CN2013102987244A CN201310298724A CN103342609A CN 103342609 A CN103342609 A CN 103342609A CN 2013102987244 A CN2013102987244 A CN 2013102987244A CN 201310298724 A CN201310298724 A CN 201310298724A CN 103342609 A CN103342609 A CN 103342609A
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- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 47
- 239000010902 straw Substances 0.000 title abstract 6
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 87
- 239000002131 composite material Substances 0.000 claims abstract description 37
- 235000016709 nutrition Nutrition 0.000 claims abstract description 28
- 241000894006 Bacteria Species 0.000 claims abstract description 22
- 239000003463 adsorbent Substances 0.000 claims abstract description 14
- 241000233866 Fungi Species 0.000 claims description 37
- 230000035764 nutrition Effects 0.000 claims description 26
- 238000002360 preparation method Methods 0.000 claims description 22
- 241000209094 Oryza Species 0.000 claims description 16
- 235000007164 Oryza sativa Nutrition 0.000 claims description 16
- 235000009566 rice Nutrition 0.000 claims description 16
- 238000002156 mixing Methods 0.000 claims description 12
- 235000012054 meals Nutrition 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 11
- 241001474374 Blennius Species 0.000 claims description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 10
- 150000001875 compounds Chemical class 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 9
- 239000012467 final product Substances 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 9
- 229920000881 Modified starch Polymers 0.000 claims description 8
- 239000004368 Modified starch Substances 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 8
- 235000019426 modified starch Nutrition 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 6
- 239000004615 ingredient Substances 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 238000000855 fermentation Methods 0.000 claims description 4
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- 230000004151 fermentation Effects 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
- 239000010452 phosphate Substances 0.000 claims description 3
- 229910052700 potassium Inorganic materials 0.000 claims description 3
- 239000011591 potassium Substances 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 235000015099 wheat brans Nutrition 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 239000011368 organic material Substances 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 5
- 238000012360 testing method Methods 0.000 abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- 230000004913 activation Effects 0.000 abstract description 2
- 239000002250 absorbent Substances 0.000 abstract 1
- 230000002745 absorbent Effects 0.000 abstract 1
- 239000000047 product Substances 0.000 description 16
- 238000009264 composting Methods 0.000 description 9
- 244000005700 microbiome Species 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a straw thoroughly-decomposing microbial inoculum capable of being preserved for a long time under a normal temperature condition. The straw thoroughly-decomposing microbial inoculum consists of the following components in parts by weight: 4-6 parts of a powdered composite microbial inoculum, 8-12 parts of a porous adsorbent, 70-80 parts of a nutritional agent and 8-12 parts of a water absorbent. Effective living bacteria preserved by the straw thoroughly-decomposing microbial inoculum are large in amount, strong in activity and fast in activation. The straw thoroughly-decomposing microbial inoculum can keep the flora activity for two years. Tests show that the amount of the effective living bacteria preserved by the straw thoroughly-decomposing microbial inoculum disclosed by the invention is more than or equal to 0.3 billion cfu/g(ml), which is far greater than the standard of the amount which is more than or equal to 0.5 cfu/g(ml) of an organic material thoroughly-decomposing agent product of the national ministry of agriculture (GB20287-2006); and the amount of the effective living bacteria can be kept to be more than or equal to 2 cfu/g(ml) after a half year of placement, more than or equal to 1 cfu/g(ml) after one year of placement, and more than or equal to 0.5 cfu/g(ml) after two years of placement.
Description
Technical field
The present invention relates to a kind of stalk decomposing microbial inoculum, be specifically related to a kind of stalk decomposing microbial inoculum that can under normal temperature condition, preserve for a long time.
Background technology
It is to utilize the synergistic result of multiple microorganism species that stalk becomes thoroughly decomposed, as fungi, bacterium, yeast and actinomycetes etc., wherein best is various cellulose-decomposing bacterias, how therefore superior microorganism flora quantity and active be directly connected to degree and the speed that stalk becomes thoroughly decomposed preserved beneficial microorganism living bacteria count amount and microbic activity and be and judge the become thoroughly decomposed key problem in technology of agent quality of stalk.General bacterial classification all is could preserve at sub-zero temperature, and the shelf time is short at normal temperatures.Need use at normal temperatures and the microorganism-decomposing product is most.How making the big and shelf time length of the living bacteria count quantity of microorganism-decomposing product at normal temperatures is the key problem in technology that is related to microorganism-decomposing product life length.
Summary of the invention
In view of above-mentioned weak point, the object of the present invention is to provide a kind of stalk decomposing microbial inoculum that can under normal temperature condition, preserve for a long time that living bacteria count quantity is big and the shelf time is long that under normal temperature condition, makes the stalk decomposing microbial inoculum.
In order to achieve the above object, the present invention has adopted following technical scheme: a kind of stalk decomposing microbial inoculum that can under normal temperature condition, preserve for a long time, and count this stalk decomposing microbial inoculum by weight ratio and be grouped into by following one-tenth:
Powdery composite fungus agent 4-6 part
Porous adsorbent 8-12 part
Nutrition agent 70-80 part
Water-retaining agent 8-12 part.
Optimum, to count by weight ratio, this stalk decomposing microbial inoculum is grouped into by following one-tenth:
5 parts of powdery composite fungus agents
10 parts of porous adsorbents
75 parts of nutrition agents
10 parts of water-retaining agents.
Described powdery composite fungus agent is that composite fungus agent and the ground rice of 1:2 mixes by mass ratio; Wherein composite fungus agent is that yeast saccharomyces cerevisiae microbial inoculum, bacillus subtilis microbial inoculum and the green wooden enzyme agent that the living bacteria count of 1:5:10 is 1-3 hundred million cfu/g mixes by mass ratio; Ground rice is that fineness is not less than 80 purpose rice meals and minces.Continuous experimental study through the applicant, finally draw the ground rice of admixture doubling dose in composite fungus agent, it has the following advantages: 1, can be fully and composite fungus agent mix, be convenient to be dispersed in the porous adsorbent uniformly, and can make more firm being adsorbed on the porous adsorbent of composite fungus agent.2, ground rice can not produce any harm to composite fungus agent, can offer one of composite fungus agent protection place on the contrary, and under becoming thoroughly decomposed environment in the later stage, ground rice can also provide necessary nutriment for the quick breeding of composite fungus agent flora.
Described porous adsorbent is selected from one or more in porous active powdered carbon, seaweed powder, the wilkinite.
Described nutrition agent is made by following preparation technology:
(1) take by weighing wheat bran and rice bran according to the l:4 mass ratio, with its mix major ingredient, standby;
(2) according to per 100 kilograms of major ingredient meters, add 2 kilograms in brown sugar, 0.5 kilogram of 1 kilogram in urea and potassium primary phosphate mix then, and it is standby to get compound;
(3) it is wet compound to be carried out blunge, and humidity is pinched with hand and agglomeratingly do not dripped, and one touches namely to loose and is advisable, and adopts conventional zymotechnique to its fermentation 3-4 days to the compound of mixing after wet then, gets nutrition agent.
Described water-retaining agent is diatomite or modified starch.
The preparation method of the stalk decomposing microbial inoculum that can preserve for a long time under normal temperature condition may further comprise the steps:
(1) porous adsorbent, water-retaining agent are at high temperature carried out sterilizing, drying respectively and handle, the airtight normal temperature that is cooled to is standby then;
(2) at first with powdery composite fungus agent and porous adsorbent mixing, add nutrition agent then, add water-retaining agent at last, be mixed into fluffy meal, namely get the stalk decomposing microbial inoculum, the pack back is placed at normal temperature and is got final product.
The present invention utilizes the porous adsorbent material to provide more habitat for beneficial microbe colony, guarantees microorganism viable bacteria number quantity; Utilize the water-retaining agent material to reduce moisture content rapidly, can make product moisture control at 10%-l5%, make microorganism under low water state, be in a kind of dormancy or semidormancy state; Adding the nutrition agent that ferments is nutritional condition to be provided and to enlarge dominant bacteria quantity rapidly in order to add under the water state, to breed fast behind the activating microorganisms flora, reaches the effect of quick composting stalk.
The living bacteria count quantity that the present invention preserves is big, and activity is strong, activation is fast.Keep its bacterial activity can be for 2 years.After testing: the stalk that the present invention forms living bacteria count 〉=300,000,000 cfu/g(ml that bacterium preserves that become thoroughly decomposed).Be far longer than the national Ministry of Agriculture (GB20287-2006) organic material composting agent product living bacteria count 〉=0.5 cfu/g(ml) standard, and place viable count 〉=2 cfu/g(ml that can remain valid after half a year); Viable count 〉=l cfu/g(ml can remain valid after 1 year); Viable count 〉=0.5 cfu/g(ml can remain valid after 2 years).This product can reach the standard of the national Ministry of Agriculture (GB20287-2006) organic material composting agent product after 2 years.And after conventional organic material composting agent product placed half a year or 1 year, the living bacteria count mortality ratio was nearly all more than 80-90%.Place after 1 year, all do not reach the standard of national Ministry of Agriculture standard (GB20287-2006) organic material composting agent product.
Embodiment
Below we the present invention is further illustrated in conjunction with specific embodiments.
Embodiment 1
The preparation of powdery composite fungus agent:
(1) preparation composite fungus agent: be yeast saccharomyces cerevisiae microbial inoculum, bacillus subtilis microbial inoculum and the green wooden enzyme agent that 1:5:10 and living bacteria count are 1-3 hundred million cfu/g with mass ratio, mix, obtain composite fungus agent.
(3) preparation ground rice: rice is pulverized to fineness is not less than 80 purpose powder, obtained ground rice.
(4) be that composite fungus agent and the ground rice of 1:2 mixes with mass ratio, obtain the powdery composite fungus agent.
The preparation of nutrition agent:
(1) take by weighing wheat bran and rice bran according to the l:4 mass ratio, with its mix major ingredient, standby;
(2) according to per 100 kilograms of major ingredient meters, add 2 kilograms in brown sugar, 0.5 kilogram of 1 kilogram in urea and potassium primary phosphate mix then, and it is standby to get compound;
(3) compound being carried out blunge wets, humidity is pinched with hand and is not agglomeratingly dripped, and one touches namely to loose and is advisable, and adopts conventional zymotechnique to its fermentation 3-4 days to the compound of mixing after wet then, get nutrition agent, the conventional zymotechnique here generally refers to closing anaerobic fermentation under the normal temperature condition.
The preparation of the stalk decomposing microbial inoculum that can under normal temperature condition, preserve for a long time:
(1) seaweed powder, diatomite are at high temperature carried out sterilizing, drying respectively and handle, the airtight normal temperature that is cooled to is standby then;
(2) at first with 40kg powdery composite fungus agent and 80kg seaweed powder mixing, add the 700kg nutrition agent then, add 80kg diatomite at last, be mixed into fluffy meal, namely get the stalk decomposing microbial inoculum, the pack back is placed at normal temperature and is got final product.
Embodiment 2
The preparation method of powdery composite fungus agent and nutrition agent is with embodiment 1
The preparation of the stalk decomposing microbial inoculum that can under normal temperature condition, preserve for a long time:
(1) seaweed powder, diatomite are at high temperature carried out sterilizing, drying respectively and handle, the airtight normal temperature that is cooled to is standby then;
(2) at first with 50kg powdery composite fungus agent and 100kg seaweed powder mixing, add the 750kg nutrition agent then, add 100kg diatomite at last, be mixed into fluffy meal, namely get the stalk decomposing microbial inoculum, the pack back is placed at normal temperature and is got final product.
Embodiment 3
The preparation method of powdery composite fungus agent and nutrition agent is with embodiment 1
The preparation of the stalk decomposing microbial inoculum that can under normal temperature condition, preserve for a long time:
(1) seaweed powder, diatomite are at high temperature carried out sterilizing, drying respectively and handle, the airtight normal temperature that is cooled to is standby then;
(2) at first with 60kg powdery composite fungus agent and 120kg seaweed powder mixing, add the 800kg nutrition agent then, add 120kg diatomite at last, be mixed into fluffy meal, namely get the stalk decomposing microbial inoculum, the pack back is placed at normal temperature and is got final product.
Embodiment 4
The preparation method of powdery composite fungus agent and nutrition agent is with embodiment 1
The preparation of the stalk decomposing microbial inoculum that can under normal temperature condition, preserve for a long time:
(1) porous activated carbon, modified starch are at high temperature carried out sterilizing, drying respectively and handle, the airtight normal temperature that is cooled to is standby then;
(2) at first with 50kg powdery composite fungus agent and 100kg porous activated carbon mixing, add the 750kg nutrition agent then, add the 100kg modified starch at last, be mixed into fluffy meal, namely get the stalk decomposing microbial inoculum, the pack back is placed at normal temperature and is got final product.
Embodiment 5
The preparation method of powdery composite fungus agent and nutrition agent is with embodiment 1
The preparation of the stalk decomposing microbial inoculum that can under normal temperature condition, preserve for a long time:
(1) wilkinite, diatomite are at high temperature carried out sterilizing, drying respectively and handle, the airtight normal temperature that is cooled to is standby then;
(2) at first with 50kg powdery composite fungus agent and 100kg wilkinite mixing, add the 750kg nutrition agent then, add 100kg diatomite at last, be mixed into fluffy meal, namely get the stalk decomposing microbial inoculum, the pack back is placed at normal temperature and is got final product.
Embodiment 6
The preparation method of powdery composite fungus agent and nutrition agent is with embodiment 1
The preparation of the stalk decomposing microbial inoculum that can under normal temperature condition, preserve for a long time:
(1) seaweed powder, modified starch are at high temperature carried out sterilizing, drying respectively and handle, the airtight normal temperature that is cooled to is standby then;
(2) at first with 50kg powdery composite fungus agent and 100kg seaweed powder mixing, add the 750kg nutrition agent then, add the 100kg modified starch at last, be mixed into fluffy meal, namely get the stalk decomposing microbial inoculum, the pack back is placed at normal temperature and is got final product.
Embodiment 7
The preparation method of powdery composite fungus agent and nutrition agent is with embodiment 1
The preparation of the stalk decomposing microbial inoculum that can under normal temperature condition, preserve for a long time:
(1) wilkinite, modified starch are at high temperature carried out sterilizing, drying respectively and handle, the airtight normal temperature that is cooled to is standby then;
(2) at first with 50kg powdery composite fungus agent and 100kg wilkinite mixing, add the 750kg nutrition agent then, add the 100kg modified starch at last, be mixed into fluffy meal, namely get the stalk decomposing microbial inoculum, the pack back is placed at normal temperature and is got final product.
Product to embodiment of the invention 1-7 has carried out following test, and test result sees Table 1
The technical indicator of the product of table 1 embodiment of the invention 1-7:
After testing: the stalk that the present invention forms living bacteria count 〉=300,000,000 cfu/g(ml that bacterium preserves that become thoroughly decomposed).Be far longer than the national Ministry of Agriculture (GB20287-2006) organic material composting agent product living bacteria count 〉=0.5 cfu/g(ml) standard, and place viable count 〉=2 cfu/g(ml that can remain valid after half a year); Viable count 〉=l cfu/g(ml can remain valid after 1 year); Viable count 〉=0.5 cfu/g(ml can remain valid after 2 years).This product can reach the standard of the national Ministry of Agriculture (GB20287-2006) organic material composting agent product after 2 years.And after conventional organic material composting agent product placed half a year or 1 year, the living bacteria count mortality ratio was nearly all more than 80-90%.Place after 1 year, all do not reach the standard of national Ministry of Agriculture standard (GB20287-2006) organic material composting agent product.
Claims (7)
1. the stalk decomposing microbial inoculum that can preserve for a long time under normal temperature condition is characterized in that: meter by weight ratio, and this stalk decomposing microbial inoculum is grouped into by following one-tenth:
Powdery composite fungus agent 4-6 part
Porous adsorbent 8-12 part
Nutrition agent 70-80 part
Water-retaining agent 8-12 part.
2. the stalk decomposing microbial inoculum that can preserve for a long time under normal temperature condition according to claim 1 is characterized in that: meter by weight ratio, and this stalk decomposing microbial inoculum is grouped into by following one-tenth:
5 parts of powdery composite fungus agents
10 parts of porous adsorbents
75 parts of nutrition agents
10 parts of water-retaining agents.
3. the stalk decomposing microbial inoculum that can preserve for a long time under normal temperature condition according to claim 1 and 2 is characterized in that: described powdery composite fungus agent is that composite fungus agent and the ground rice of 1:2 mixes by mass ratio; Wherein composite fungus agent is that yeast saccharomyces cerevisiae microbial inoculum, bacillus subtilis microbial inoculum and the green wooden enzyme agent that the living bacteria count of 1:5:10 is 1-3 hundred million cfu/g mixes by mass ratio; Ground rice is that fineness is not less than 80 purpose rice meals and minces.
4. the stalk decomposing microbial inoculum that can preserve for a long time under normal temperature condition according to claim 1 and 2, it is characterized in that: described porous adsorbent is selected from one or more in porous active powdered carbon, seaweed powder, the wilkinite.
5. the stalk decomposing microbial inoculum that can under normal temperature condition, preserve for a long time according to claim 1 and 2, it is characterized in that: described nutrition agent is made by following preparation technology:
(1) take by weighing wheat bran and rice bran according to the l:4 mass ratio, with its mix major ingredient, standby;
(2) according to per 100 kilograms of major ingredient meters, add 2 kilograms in brown sugar, 0.5 kilogram of 1 kilogram in urea and potassium primary phosphate mix then, and it is standby to get compound;
(3) it is wet compound to be carried out blunge, and humidity is pinched with hand and agglomeratingly do not dripped, and one touches namely to loose and is advisable, and adopts conventional zymotechnique to its fermentation 3-4 days to the compound of mixing after wet then, gets nutrition agent.
6. the stalk decomposing microbial inoculum that can preserve for a long time under normal temperature condition according to claim 1 and 2, it is characterized in that: described water-retaining agent is diatomite or modified starch.
7. the preparation method of the stalk decomposing microbial inoculum that can under normal temperature condition, preserve for a long time as claimed in claim 1 or 2, it is characterized in that: this preparation method may further comprise the steps:
(1) porous adsorbent, water-retaining agent are at high temperature carried out sterilizing, drying respectively and handle, the airtight normal temperature that is cooled to is standby then;
(2) at first with powdery composite fungus agent and porous adsorbent mixing, add nutrition agent then, add water-retaining agent at last, be mixed into fluffy meal, namely get the stalk decomposing microbial inoculum, the pack back is placed at normal temperature and is got final product.
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| CN106380240A (en) * | 2016-08-29 | 2017-02-08 | 陈景河 | Organic fertilizer decomposing agent taking activated oyster shell powder as carrier |
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| CN103667089B (en) * | 2013-12-05 | 2016-03-30 | 中国农业科学院农业资源与农业区划研究所 | Take rural organic wastes as microbial inoculum and the application thereof that fermentation raw material prepares fertilizer |
| CN106380240A (en) * | 2016-08-29 | 2017-02-08 | 陈景河 | Organic fertilizer decomposing agent taking activated oyster shell powder as carrier |
| CN108148769A (en) * | 2018-03-19 | 2018-06-12 | 临沂大学 | A kind of room temperature preserves culture medium of Arthrinium fungi strain and its preparation method and application |
| CN108148769B (en) * | 2018-03-19 | 2020-11-27 | 临沂大学 | Culture medium for normal-temperature preservation of arthrobotrys fungus strains and preparation method and application thereof |
| CN108585974A (en) * | 2018-04-20 | 2018-09-28 | 常州市蒽盗钟情生物科技有限公司 | A kind of preparation method of the odorless straw decomposing inoculant of fixed nitrogen |
| CN108997034A (en) * | 2018-08-10 | 2018-12-14 | 成都惠森生物技术有限公司 | Decomposed starting agent of one kind and preparation method thereof |
| CN110028343A (en) * | 2019-03-28 | 2019-07-19 | 环创(厦门）科技股份有限公司 | A kind of fruits and vegetables waste dish waste disposal method |
| CN110054525A (en) * | 2019-04-08 | 2019-07-26 | 河北新世纪周天生物科技有限公司 | A kind of biological water-keeping agent and its preparation process using corn stover as primary raw material |
| CN112552109A (en) * | 2020-12-08 | 2021-03-26 | 河北邢化生态环境工程有限公司 | Diatom carbon microbial compound microbial agent and preparation and application thereof |
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