CN103667089A - Bacterial agent for preparing organic fertilizer by adopting countryside organic waste as fermentation raw material, and application thereof - Google Patents
Bacterial agent for preparing organic fertilizer by adopting countryside organic waste as fermentation raw material, and application thereof Download PDFInfo
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Abstract
The present invention discloses a bacterial agent for preparing an organic fertilizer by adopting countryside organic waste as a fermentation raw material, and an application thereof, wherein the active components of the bacterial agent comprise Trichoderma viride and Saccharomyces cerevisiae. According to the present invention, the organic fertilizer prepared from the bacterial agent adopting the countryside organic waste as the fermentation raw material provides significant yield increase effects for cucumber and green pepper, wherein the yield of the cucumber can be increase by 21.98%, and the yield of the green pepper can be increase by 19.71%; and application of the organic fertilizer preparation bacterial agent adopting the countryside organic waste as the fermentation raw material to prepare the organic fertilizer is the effective approach for countryside organic waste resource utilization, and the fermentation process is simple and easy to perform, and is suitable for promotion and application in the countryside areas.
Description
Technical field
The present invention relates to take rural organic wastes and prepare microbial inoculum and the application thereof of fertilizer as fermentation raw material.
Background technology
Current due to the dealing with improperly of the rural organic wastes such as the organic composition that becomes thoroughly decomposed in agricultural crop straw, livestock and poultry cultivation ight soil, rural area solid life refuse (the residual body of rubbish from cooking, melon and fruit peel and vegetables), the problem of environmental pollution causing is very serious.How these rural organic wastes are effectively processed, alleviated its impact on environment, become a problem demanding prompt solution.
The rotten fermentation of heap is under certain processing condition, and organic waste is fermented, becomes thoroughly decomposed, degraded, and makes it to become stable organic matter, and utilizes the heat producing in fermenting process to kill the Biochemical processes that harmful microorganism reaches harmless treatment.The effect of the organic fertilizer that the rotten fermentation of heap obtains depends on the rotten fermenting agent of adopted heap.
Summary of the invention
Technical problem to be solved by this invention is to provide and is a kind ofly applicable to take rural organic wastes and prepares the microbial inoculum of fertilizer as fermentation raw material.
Microbial inoculum for the preparation of fertilizer provided by the present invention, its activeconstituents is comprised of viride and yeast saccharomyces cerevisiae.
In the above-mentioned microbial inoculum for the preparation of fertilizer, described viride is at the ACCC30206 that is numbered at China Committee for Culture Collection of Microorganisms agricultural microorganism center, and described yeast saccharomyces cerevisiae is at the ACCC20065 that is numbered at China Committee for Culture Collection of Microorganisms agricultural microorganism center.
In the above-mentioned microbial inoculum for the preparation of fertilizer, the colony-forming unit of described viride and described yeast saccharomyces cerevisiae (cfu) number ratio can be (1-2): (1-2), and as 1:1.
The above-mentioned microbial inoculum for the preparation of fertilizer also can comprise carrier.Described carrier can be solid carrier or liquid vehicle.Described solid carrier is mineral material, biomaterial or macromolecular compound; Described mineral material is at least one in clay, talcum, kaolin, montmorillonite, white carbon, zeolite, silica, turfy soil and diatomite; Described biomaterial is at least one in the ight soil of the stalk of all kinds of crops, loose shell, straw, Pericarppium arachidis hypogaeae, Semen Maydis powder, bean powder, starch, the peat composed of rotten mosses and animal; Described macromolecular compound is polyvinyl alcohol and/or polyglycol.Described liquid vehicle can be organic solvent, vegetables oil, mineral oil or water; Described organic solvent is decane and/or dodecane.In described microbial inoculum, described viride and described yeast saccharomyces cerevisiae can exist with the form of the filtrate of the fermented liquid of viable cell, the viable cell of being cultivated, cell culture or the mixture of cell and filtrate.The formulation of described microbial inoculum can be multiple formulation, as liquor, emulsion, suspension agent, pulvis, granule, wettable powder or water dispersible granules.
The above-mentioned microbial inoculum for the preparation of fertilizer also belongs to protection scope of the present invention take the application that rural organic wastes prepared in fertilizer as fermentation raw material.
In above-mentioned application, described rural organic wastes is the organic composition that becomes thoroughly decomposed in agricultural crop straw, livestock and poultry cultivation ight soil and rural area solid life refuse.
Organic mixture that can be the residual body of vegetables, melon and fruit peel and rubbish from cooking that becomes thoroughly decomposed in the solid life refuse of described rural area.
The invention provides a kind of concrete method of preparing fertilizer.
The method of preparing fertilizer provided by the present invention, comprise the organic composition that becomes thoroughly decomposed in agricultural crop straw, livestock and poultry cultivation ight soil and rural area solid life refuse is mixed to get to the rotten fermentation raw material of heap, in the rotten fermentation raw material of described heap, add described microbial inoculum to obtain piling body material, described heap body material is made to heap body and ferment and obtain fertilizer.
In the above-mentioned method of preparing fertilizer, the mass ratio of the organic composition that becomes thoroughly decomposed in described agricultural crop straw, described livestock and poultry cultivation ight soil and described rural area solid life refuse is 1:2.5:1.5, wherein, the quality of described agricultural crop straw be take water content as 15%, the quality of described livestock and poultry cultivation ight soil is in water content 30%, and the quality of the organic composition that becomes thoroughly decomposed in the solid life refuse of described rural area be take water content as 45%.
In the above-mentioned method of preparing fertilizer, the proportioning of the rotten fermentation raw material of described heap and described microbial inoculum is that in every kilogram of rotten fermentation raw material of described heap, to add total thalline content be 5 * 10
9the described microbial inoculum of cfu.
In the above-mentioned method of preparing fertilizer, described heap body can be rectangular parallelepiped, and the length of described heap body is 6 meters, and wide is 3 meters, and height can be 1.4 meters-1.6 meters (as 1.5 meters).
In the above-mentioned method of preparing fertilizer, described fermentation is that 20-35 ℃ (as 30-35 ℃), relative humidity are that 50%-80%(is as 60% in temperature) environment in ferment 21 days, turning in every 7 days.
In the above-mentioned method of preparing fertilizer, the organic mixture that can be the residual body of vegetables, melon and fruit peel and rubbish from cooking that becomes thoroughly decomposed in the solid life refuse of described rural area.
In an embodiment of the invention, described agricultural crop straw is maize straw, described livestock and poultry cultivation ight soil is cow dung, and the organic composition that becomes thoroughly decomposed in the solid life refuse of described rural area is mixed according to the mass ratio of 1:2:2 by the residual body of vegetables, melon and fruit peel and rubbish from cooking.Wherein, the residual body of vegetables is Collina and discarded Chinese cabbage leaf, and melon and fruit peel is Watermelon rind, and rubbish from cooking is leftovers and leftovers; The quality of the residual body of vegetables, melon and fruit peel and rubbish from cooking is with 45% water content meter.
The present invention also provides a kind of method of concrete raising cultivated plant output.
The method of raising cultivated plant output provided by the present invention, comprises in the soil of described cultivated plant and applies above-mentioned fertilizer, improves the output of described cultivated plant.
In the method for above-mentioned raising cultivated plant output, described raising cultivated plant output can be and improves cucumber yield (cucumber fruits output) or improve yield of hot pepper (pepper fruit output).
In the method for above-mentioned raising cultivated plant output, when cultivated plant is cucumber or capsicum, the consumption of described fertilizer is 10 grams of described fertilizers of cultivated plant described in every strain.
In above-mentioned application, the mass ratio of the organic composition that becomes thoroughly decomposed in described agricultural crop straw, described livestock and poultry cultivation ight soil and described rural area solid life refuse can be 1:2.5:1.5, wherein, the quality of described agricultural crop straw be take water content as 15%, the quality of described livestock and poultry cultivation ight soil is in water content 30%, and the quality of the organic composition that becomes thoroughly decomposed in the solid life refuse of described rural area be take water content as 45%.
In an embodiment of the invention, described agricultural crop straw is maize straw, described livestock and poultry cultivation ight soil is cow dung, and the organic composition that becomes thoroughly decomposed in the solid life refuse of described rural area is mixed according to the mass ratio of 1:2:2 by the residual body of vegetables, melon and fruit peel and rubbish from cooking.Wherein, the residual body of vegetables is Collina and discarded Chinese cabbage leaf, and melon and fruit peel is Watermelon rind, and rubbish from cooking is leftovers and leftovers; The quality of the residual body of vegetables, melon and fruit peel and rubbish from cooking is with 45% water content meter.
Described raising cultivated plant output can be and improves cucumber yield (cucumber fruits output) or improve yield of hot pepper (pepper fruit output)
Experiment showed, that the microbial inoculum for the preparation of fertilizer of the present invention take the fertilizer that rural organic wastes prepared as fermentation raw material cucumber and green pepper are all had to significant effect of increasing production, can make cucumber production promoting is 21.98%, can make green pepper volume increase 19.71%.Utilizing the microbial inoculum for the preparation of fertilizer of the present invention to take rural organic wastes, as fermentation raw material, to prepare fertilizer be rural organic wastes to be carried out to the effective way of recycling, and this zymotechnique is simple and easy to do, be very suitable for applying in Rural areas.
Embodiment
Below in conjunction with embodiment, the present invention is further described in detail, the embodiment providing is only in order to illustrate the present invention, rather than in order to limit the scope of the invention.Experimental technique in following embodiment, if no special instructions, is ordinary method.In following embodiment, material used, reagent etc., if no special instructions, all can obtain from commercial channels.
Viride (Trichoderma viride) ACCC30206 and yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20065 used in following embodiment were all concealed in China Committee for Culture Collection of Microorganisms agricultural microorganism center (abbreviation ACCC before the application's the applying date, address: No.12 ,zhongguancun south street,Haidian District, Beijing, INST OF AGRICULTURAL RESOURCES, postcode 100081), viride (Trichoderma viride) ACCC30206 and yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20065 are July 26 nineteen ninety collection day, from collection, the public can obtain this two bacterial strains from Chinese microorganism strain preservation management committee agricultural microorganism center.
The nutrient contents such as the full nitrogen of all samples in following embodiment, full phosphorus, full potassium, organic matter detect according to organic fertilizer standard NY525-2012, and total nutrient content refers to full nitrogen, full phosphorus, potassium content sum entirely.
Cucumber variety in following embodiment is match storehouse, and green pepper kind is red Susan (Li Shigui, Wang Fei, Gu Jingang, Zhu Changxiong.Application and the field test effect thereof of microbiobacterial agent in agricultural wastes compost maturity process.JOURNAL OF MICROBIOLOGY, 2011,31(6): 62-65.), by Vegetable & Flower Inst., Chinese Academy of Agriculture Science, providing, public Ke Cong Chinese Academy of Agricultural Sciences's agricultural resource and agricultural regionalization institute obtain, and this biomaterial related experiment of the present invention of only attaching most importance to is again used, not can be used as other purposes and uses.
Embodiment 1, for the preparation of the microbial inoculum of fertilizer
For the preparation of the microbial inoculum of fertilizer, in this application also referred to as organic material composting microbial inoculum, the present embodiment has been prepared three kinds of organic material composting microbial inoculums, i.e. organic material composting microbial inoculum A, organic material composting microbial inoculum B and organic material composting microbial inoculum C.Its preparation method is as follows:
By viride (Trichoderma viride) ACCC30206 be seeded in PDA medium slant, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20065 is seeded in GPYA medium slant, be placed in 28 ℃ of incubator activation culture 2-3 days, obtain viride (Trichoderma viride) ACCC30206 of activation and yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20065 of activation.
By yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) the ACCC20065 access GPY substratum of viride (Trichoderma viride) the ACCC30206 access PD substratum of activation, activation, all at 28 ℃, 200r/min shaking culture 3 days, obtains respectively viride (Trichoderma viride) ACCC30206 fermented liquid (5 * 10
9cfu/ml) and yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20065 fermented liquid (5 * 10
9cfu/ml).Viride (Trichoderma viride) ACCC30206 fermented liquid and yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20065 fermented liquid are mixed to get to organic material composting microbial inoculum A according to the colony-forming unit of 1:1 (cfu) number ratio.In this organic material composting microbial inoculum A, the content of viride (Trichoderma viride) ACCC30206 is 2.5 * 10
9cfu/ml, the content of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20065 is 2.5 * 10
9cfu/ml.Viride (Trichoderma viride) ACCC30206 fermented liquid and yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20065 fermented liquid are mixed to get to organic material composting microbial inoculum B according to the colony-forming unit of 1:2 (cfu) number ratio.In this organic material composting microbial inoculum B, the content of viride (Trichoderma viride) ACCC30206 is 1.7 * 10
9cfu/ml, the content of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20065 is 3.3 * 10
9cfu/ml.Viride (Trichoderma viride) ACCC30206 fermented liquid and yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20065 fermented liquid are mixed to get to organic material composting microbial inoculum C according to the colony-forming unit of 2:1 (cfu) number ratio.In this organic material composting microbial inoculum C, the content of viride (Trichoderma viride) ACCC30206 is 3.3 * 10
9cfu/ml, the content of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20065 is 1.7 * 10
9cfu/ml.
Wherein, the preparation method of substratum is as follows:
PD substratum: the potato of potato 200g(peeling is cut into small pieces, boiled and within 30 minutes, crossed elimination filtrate), glucose 20g, with distilled water, be settled to 1000ml, 121 ℃ of vapor sterilization 20min.
PDA substratum: the potato of potato 200g(peeling is cut into small pieces, boiled and within 30 minutes, crossed elimination filtrate), glucose 20g, agar 18-20g, is settled to 1000ml with distilled water, 121 ℃ of vapor sterilization 20min.
GPY substratum: glucose 40g, peptone 5g, yeast extract paste 5g, be settled to 1000ml with distilled water, 121 ℃ of vapor sterilization 20min.
GPYA substratum: glucose 40g, peptone 5g, yeast extract paste 5g, agar 18-20g, be settled to 1000ml with distilled water, 121 ℃ of vapor sterilization 20min.
Embodiment 2, utilize the organic material composting microbial inoculum of embodiment 1, organic composition that becomes thoroughly decomposed of take in agricultural crop straw, livestock and poultry cultivation ight soil and rural area solid life refuse, as the rotten fermentation raw material of heap, adopts the rotten fermentation of three forms heaps to prepare organic fertilizer
1, pile the preparation of rotten fermentation raw material
Organic composition that becomes thoroughly decomposed in the rural area solid life refuse adopting in this step is mixed according to the mass ratio of 1:2:2 by the residual body of vegetables, melon and fruit peel and rubbish from cooking.Wherein, the residual body of vegetables is Collina and discarded Chinese cabbage leaf, and melon and fruit peel is Watermelon rind, and rubbish from cooking is leftovers and leftovers; The quality of the residual body of vegetables, melon and fruit peel and rubbish from cooking is with 45% water content meter.
This step by maize straw and organic in the solid life refuse of rural area become thoroughly decomposed into cut into the fritter that length is 2.0cm after and cow dung be mixed to get the rotten fermentation raw material of heap.The rotten fermentation raw material of this heap is that the organic composition that becomes thoroughly decomposed in maize straw, cow dung and rural area solid life refuse is mixed to get according to the mass ratio of 1:2.5:1.5.
Wherein, the water content of above-mentioned maize straw is 15%, and the water content of cow dung is 30%, and the water content of the organic composition that becomes thoroughly decomposed in the solid life refuse of rural area is 45%.The nutrient content of three kinds of rotten raw materials of heap is as shown in table 1.
The nutrient content (in butt) of table 1, three kinds of rotten raw materials of heap
2, the preparation of heap body material
In the rotten fermentation raw material of heap of step 1, add respectively machine material decomposing microbial inoculum A, organic material composting microbial inoculum B and organic material composting microbial inoculum C to make heap body material A, heap body material B and heap body material C, concrete method is as follows:
The preparation of 2.1 heap body material A
According to every kilogram of rotten fermentation raw material of heap, adding total thalline content is 5 * 10
9cfu(is that the thalline content of viride (Trichoderma viride) ACCC30206 and the thalline content sum of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20065 are 5 * 10
9cfu) proportioning of the organic material composting microbial inoculum A of embodiment 1 adds the organic material composting microbial inoculum A of embodiment 1 to obtain piling body material A in the rotten fermentation raw material of heap of step 1.
The preparation of 2.2 heap body material B
According to every kilogram of rotten fermentation raw material of heap, adding total thalline content is 5 * 10
9cfu(is that the thalline content of viride (Trichoderma viride) ACCC30206 and the thalline content sum of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20065 are 5 * 10
9cfu) proportioning of the organic material composting microbial inoculum B of embodiment 1 adds the organic material composting microbial inoculum B of embodiment 1 to obtain piling body material B in the rotten fermentation raw material of heap of step 1.
The preparation of 2.3 heap body material C
According to every kilogram of rotten fermentation raw material of heap, adding total thalline content is 5 * 10
9cfu(is that the thalline content of viride (Trichoderma viride) ACCC30206 and the thalline content sum of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20065 are 5 * 10
9cfu) proportioning of the organic material composting microbial inoculum C of embodiment 1 adds the organic material composting microbial inoculum C of embodiment 1 to obtain piling body material C in the rotten fermentation raw material of heap of step 1.
3, heap body material being made to heap body carries out the rotten fermentation of three forms heaps and obtains organic fertilizer
The rotten fermentation of heap is carried out in fermentation cabin, this fermentation cabin comprises Cang Ding, at the bottom of bulkhead and storehouse, a plurality of lattice walls are set at the bottom of perpendicular to storehouse at the bottom of storehouse, fermentation cabin is divided into 9 lattice with lattice wall by volume, be that lattice wall is divided into 3 three lattice groups by described fermentation cabin, each three lattice group is comprised of three adjacent lattice, under the storehouse of every lattice, be longitudinally evenly distributed the ventilation slot of twice length * wide * height=6m * 0.15m * 0.13m, on per pass ventilation slot, lay one of stainless-steel sheet with holes, the length of every stainless-steel sheet * wide * thick=6m * 0.19m * 0.003m, on every stainless-steel sheet, being evenly distributed the ventilating pit that 400 diameters are 0.012m is beneficial to ventilated, on ventilation slot, complete after stainless-steel sheet, steel plate top is just with concordant at the bottom of storehouse.Three adjacent lattice are three lattice groups, and this fermentation cabin is divided into 3 three lattice groups, are respectively three lattice group A, three lattice group B and three lattice group C.
Prepare by the following method organic fertilizer A, organic fertilizer B and organic fertilizer C simultaneously.
The preparation of 3.1 organic fertilizer A
To adding water in the heap body material A of 2.1 preparations, the water content of heap body material A is all adjusted to 60%, heap body material A is made to the heap body of length * wide * height=6m * 3m * 1.5m at the first lattice of three lattice group A, in temperature, be 30-35 ℃, relative humidity is standing for fermentation 7 days in 60% environment, obtain fermentation heap body material A for the first time, the second lattice that fermentation heap body material A overturning is for the first time entered to three lattice group A are made the heap body of length * wide * height=6m * 3m * 1.5m, in temperature, be 30-35 ℃, relative humidity is standing for fermentation 7 days in 60% environment, obtain fermentation heap body material A for the second time, the 3rd lattice that fermentation heap body material A overturning is for the second time entered to three lattice group A are made the heap body of length * wide * height=6m * 3m * 1.5m, in temperature, be 30-35 ℃, relative humidity is standing for fermentation 7 days in 60% environment, obtain organic fertilizer A.
The preparation of 3.2 organic fertilizer B
Except A is replaced with B, all the other are completely with 3.1.Concrete grammar is as follows: to adding water in the heap body material B of 2.2 preparations, the water content of heap body material B is all adjusted to 60%, heap body material B is made to the heap body of length * wide * height=6m * 3m * 1.5m at the first lattice of three lattice group B, in temperature, be 30-35 ℃, relative humidity is standing for fermentation 7 days in 60% environment, obtain fermentation heap body material B for the first time, the second lattice that fermentation heap body material B overturning is for the first time entered to three lattice group B are made the heap body of length * wide * height=6m * 3m * 1.5m, in temperature, be 30-35 ℃, relative humidity is standing for fermentation 7 days in 60% environment, obtain fermentation heap body material B for the second time, the 3rd lattice that fermentation heap body material B overturning is for the second time entered to three lattice group B are made the heap body of length * wide * height=6m * 3m * 1.5m, in temperature, be 30-35 ℃, relative humidity is that in 60% environment, standing for fermentation obtains organic fertilizer B for 7 days.
The preparation of 3.3 organic fertilizer C
Except A is replaced with C, all the other are completely with 3.1.Concrete grammar is as follows: to adding water in the heap body material C of 2.3 preparations, the water content of heap body material C is all adjusted to 60%, heap body material C is made to the heap body of length * wide * height=6m * 3m * 1.5m at the first lattice of three lattice group C, in temperature, be 30-35 ℃, relative humidity is standing for fermentation 7 days in 60% environment, obtain fermentation heap body material C for the first time, the second lattice that fermentation heap body material C overturning is for the first time entered to three lattice group C are made the heap body of length * wide * height=6m * 3m * 1.5m, in temperature, be 30-35 ℃, relative humidity is standing for fermentation 7 days in 60% environment, obtain fermentation heap body material C for the second time, the 3rd lattice that fermentation heap body material C overturning is for the second time entered to three lattice group C are made the heap body of length * wide * height=6m * 3m * 1.5m, in temperature, be 30-35 ℃, relative humidity is standing for fermentation 7 days in 60% environment, obtain organic fertilizer C.
4, results and analysis
The nutrient content of sample before and after the rotten fermentation of 4.1 heaps
As shown in table 2, organic fertilizer A compares with heap body material A, and content of tatal phosphorus significantly increases, and amplification is 6.79%; And organic, full nitrogen and full potassium content decrease, the range of decrease is respectively 6.26%, 4.35% and 5.23%; But total nutrient content increases to some extent.Organic fertilizer B compares with heap body material B, and content of tatal phosphorus has increased slightly; And organic, full nitrogen and full potassium content decrease, the range of decrease is respectively 8.23%, 9.52% and 8.51%; Total nutrient content declines 3.39%.Organic fertilizer C compares with heap body material C, and content of tatal phosphorus slightly increases; And organic, full nitrogen and full potassium content decrease, the range of decrease is respectively 9.81%, 9.52% and 9.29%; Total nutrient content declines 4.42%.
The nutrient content (in butt) of sample before and after table 2, the rotten fermentation of heap
| Sample | Organic % | Full nitrogen (N) % | Full phosphorus (P) % | Full potassium (K) % | Total nutrient % |
| Heap body material A | 52.6 | 1.84 | 3.24 | 1.53 | 6.61 |
| Organic fertilizer A | 49.5 | 1.76 | 3.46 | 1.45 | 6.67 |
| Heap body material B | 52.6 | 1.84 | 3.24 | 1.53 | 6.61 |
| Organic fertilizer B | 48.6 | 1.69 | 3.26 | 1.41 | 6.36 |
| Heap body material C | 52.6 | 1.84 | 3.24 | 1.53 | 6.61 |
| Organic fertilizer C | 47.9 | 1.68 | 3.25 | 1.40 | 6.33 |
The field fertilizer efficiency experiment of 4.2 organic fertilizer
Field test is carried out at Chinese Academy of Agricultural Sciences's Langfang Experimental Base, and the cucumber variety of cultivation is match storehouse, and green pepper kind is red Susan.
Cucumber cultivation experiment is identical with the experimental design method of green pepper cultivation experiments, all as follows: experiment adopts randomized block design, 3 iterons are set, each iteron arranges 4 communities at random, is respectively CK treatment zone, organic fertilizer A treatment zone, organic fertilizer B treatment zone, organic fertilizer C treatment zone.The area of each community is 30m2.
Except the kind difference of the fertilizer of spreading manuer in holes in soil, each other field management of processing is all identical.Contrast organic fertilizer is in temperature, to be 30-35 ℃ by the rotten fermentation raw material of heap of step 1 preparation of embodiment 1, relative humidity is standing spontaneous fermentation turning after 7 days in 60% environment, in temperature, be 30-35 ℃ again, relative humidity is standing spontaneous fermentation turning after 7 days in 60% environment, in temperature, be 30-35 ℃ again, the organic fertilizer that in the environment that relative humidity is 60%, standing spontaneous fermentation obtained after 7 days.In each community respectively when transplanting, earthing field planting again after every vegetable seedling rhizosphere is spreaded manuer in holes 10 grams of fertilizers around.Wherein, CK treatment zone is contrast, and the fertilizer of executing is contrast organic fertilizer; The fertilizer that organic fertilizer A executes treatment zone is organic fertilizer A; The fertilizer that organic fertilizer B executes treatment zone is organic fertilizer B; The fertilizer that organic fertilizer C executes treatment zone is organic fertilizer C.In the harvesting time of cucumber, investigate the output (abbreviation cucumber yield) of cucumber fruits, in the harvesting time of capsicum, investigate the output (abbreviation yield of hot pepper) of pepper fruit.With t-Test, carry out significance of difference analysis.
Result shows to compare with contrasting organic fertilizer, organic fertilizer A, organic fertilizer B and the obvious effect of increasing production of organic fertilizer C to cucumber and green pepper, organic fertilizer A is 21.98%(table 3 to the effect of increasing production of cucumber), to the effect of increasing production of green pepper, be 19.71%(table 4); Organic fertilizer B is 16.57%(table 3 to the effect of increasing production of cucumber), to the effect of increasing production of green pepper, be 14.31%(table 4); Organic fertilizer C is 15.88%(table 3 to the effect of increasing production of cucumber), to the effect of increasing production of green pepper, be 13.67%(table 4).Organic fertilizer A to the obvious effect of increasing production of cucumber and green pepper higher than organic fertilizer B and organic fertilizer C, organic fertilizer B and organic fertilizer C to the effect of increasing production of cucumber and green pepper without significant difference.
Effect of increasing production to cucumber after table 3, the rotten fermentation of countryside wastes heap
| Process | Cell production (kg/ community) | Effect of increasing production (%) | The significance of difference |
| Organic fertilizer A | 106.0 | 21.98 | C |
| Organic fertilizer B | 101.3 | 16.57 | B |
| Organic fertilizer C | 100.7 | 15.88 | B |
| CK | 86.9 | / | A |
Note: in table, data are mean value, in the significance of difference, between the different processing of letter, there were significant differences (P < 0.05), between the processing that in the significance of difference, letter is identical without significant difference (P > 0.05).
The effect of increasing production of sample to green pepper after table 4, the rotten fermentation of countryside wastes heap
| Process | Cell production (kg/ community) | Effect of increasing production (%) | The significance of difference |
| Organic fertilizer A | 75.3 | 19.71 | C |
| Organic fertilizer B | 71.9 | 14.31 | B |
| Organic fertilizer C | 71.5 | 13.67 | B |
| CK | 62.9 | / | A |
Note: in table, data are mean value, in the significance of difference, between the different processing of letter, there were significant differences (P < 0.05), between the processing that in the significance of difference, letter is identical without significant difference (P > 0.05).
Claims (10)
1. for the preparation of the microbial inoculum of fertilizer, its activeconstituents is comprised of viride and yeast saccharomyces cerevisiae.
2. microbial inoculum according to claim 1, it is characterized in that: described viride is at the ACCC30206 that is numbered at China Committee for Culture Collection of Microorganisms agricultural microorganism center, and described yeast saccharomyces cerevisiae is at the ACCC20065 that is numbered at China Committee for Culture Collection of Microorganisms agricultural microorganism center.
3. microbial inoculum according to claim 1 and 2, is characterized in that: the colony-forming unit of described viride and described yeast saccharomyces cerevisiae (cfu) number is than being (1-2): (1-2).
4. according to the microbial inoculum described in claim 1,2 or 3, it is characterized in that: the colony-forming unit of described viride and described yeast saccharomyces cerevisiae (cfu) number is than being 1:1.
5. in claim 1 to 4, arbitrary described microbial inoculum is prepared the application in fertilizer as fermentation raw material take rural organic wastes.
6. application according to claim 5, is characterized in that: described rural organic wastes is the organic composition that becomes thoroughly decomposed in agricultural crop straw, livestock and poultry cultivation ight soil and rural area solid life refuse.
7. the method for preparing fertilizer, comprise the organic composition that becomes thoroughly decomposed in agricultural crop straw, livestock and poultry cultivation ight soil and rural area solid life refuse is mixed to get to the rotten fermentation raw material of heap, to adding in the rotten fermentation raw material of described heap arbitrary described microbial inoculum in claim 1 to 4 to obtain piling body material, described heap body material is made to heap body and ferment and obtain fertilizer.
8. fertilizer according to claim 7, it is characterized in that: the mass ratio of the organic composition that becomes thoroughly decomposed in described agricultural crop straw, described livestock and poultry cultivation ight soil and described rural area solid life refuse is 1:2.5:1.5, wherein, the quality of described agricultural crop straw be take water content as 15%, the quality of described livestock and poultry cultivation ight soil is in water content 30%, and the quality of the organic composition that becomes thoroughly decomposed in the solid life refuse of described rural area be take water content as 45%.
9. according to the fertilizer described in claim 7 or 8, it is characterized in that: the proportioning of the rotten fermentation raw material of described heap and described microbial inoculum is that in every kilogram of rotten fermentation raw material of described heap, to add total thalline content be 5 * 10
9the described microbial inoculum of cfu.
10. improve the method for cultivated plant output, comprise in the soil of described cultivated plant and apply the fertilizer that in claim 7-9 prepared by arbitrary described method, improve the output of described cultivated plant.
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