CN103320369B - Composite inoculant for promoting decomposing of cellulose organic wastes, and preparation method thereof - Google Patents
Composite inoculant for promoting decomposing of cellulose organic wastes, and preparation method thereof Download PDFInfo
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- CN103320369B CN103320369B CN201310292272.9A CN201310292272A CN103320369B CN 103320369 B CN103320369 B CN 103320369B CN 201310292272 A CN201310292272 A CN 201310292272A CN 103320369 B CN103320369 B CN 103320369B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention discloses a composite inoculant for promoting the decomposing of cellulose organic wastes, and a preparation method thereof. The active components of the inoculant for promoting the decomposing of cellulose organic wastes comprise Sporotrichum thermophile, Trichoderma konigii, Phanerochaete chrysosporium, Aspergillus niger, Saccharomyces cerevisiae, Bacillus subtilis and Streptomyces ambofaciens. Experiments in the invention prove that the composite inoculant for promoting the decomposing of cellulose organic wastes can effectively improve the decomposing speed of the organic wastes, and can be used for producing biological organic fertilizers.
Description
Technical field
The present invention relates to biological technical field, particularly relate to a kind of promote organic waste to become thoroughly decomposed composite fungus agent and preparation method.
Background technology
Agriculture production and the light industry such as food, extraction using alcohol produce the organic waste such as crop material, acid-sludge, schlempe of enormous amount every year, the difficult decomposed substances such as the most rich cellulose of this kind of waste, xylogen, cause these organic waste utilization ratios not high, great majority all as " rubbish " process, not only contaminate environment but also cause the wasting of resources.The microbial inoculum being commonly used to process organic waste at present is both at home and abroad mostly single microbial inoculum and the spontaneous fermentation thing microbial inoculum of purifying.The subject range of bacterium to growth conditions of the single microbial inoculum of purifying is narrow, requires high, and be easily subject to pollution and the suppression of other bacterium to fermentation condition; Under losing natural condition, be in coexisting of other bacterium of conspiracy relation for a long time, the capacity of decomposition of its organic waste reduces greatly.Bacterial classification contained in spontaneous fermentation thing microbial inoculum is the Duo Jun association gathered disorderly in natural passive fermenting process, cannot grasp its bacterial classification composition, lacks scientific, and result of use is difficult to control; The fermenting process of spontaneous fermentation thing microbial inoculum follows natural circulation law, and its decomposition efficiency is low, and effect is unstable.
Summary of the invention
An object of the present invention is to provide a kind of microbial inoculum promoting composting material to become thoroughly decomposed.
The microbial inoculum that promotion composting material provided by the invention becomes thoroughly decomposed, its activeconstituents is thermophilic fungus destroyed wire (Sporotrichumthermophile), healthy and free from worry wood mould (Trichoderma konigii), whiterot fungi (Phanerochaetechrysosporium), aspergillus niger (Aspergillus niger), yeast saccharomyces cerevisiae (Saccharomycescerevisiae), subtilis (Bacillus subtilis) CICC and product dyadic streptomycete (Streptomyces ambofaciens) ACCC.
Can be specifically: thermophilic fungus destroyed wire (Sporotrichum thermophile) CICC2441, healthy and free from worry wood mould (Trichoderma konigii) CGMCC3.04001, whiterot fungi (Phanerochaete chrysosporium) CGMCC5.00776, aspergillus niger (Aspergillus niger) CGMCC3.3147, yeast saccharomyces cerevisiae (Saccharomycescerevisiae) CICC1415, subtilis (Bacillus subtilis) CICC10076 and product dyadic streptomycete (Streptomyces ambofaciens) ACCC40133.
In above-mentioned microbial inoculum, the living bacteria count of described microbial inoculum is greater than 0.5 × 10
8requirement in the standard " agricultural microbial agent national standard (GB20287-2006) " of cfu/g(agricultural microbial preparation, the living bacteria count of organic material composting solid fungicide need be greater than 0.5 × 10
8cfu/g).In microbial inoculum, described thermophilic fungus destroyed wire (being specially thermophilic fungus destroyed wire CICC2441), described healthy and free from worry wood mould (being specially the mould CGMCC3.04001 of healthy and free from worry wood), described whiterot fungi (being specially whiterot fungi CGMCC5.00776), described aspergillus niger (being specially aspergillus niger CGMCC3.3147), described yeast saccharomyces cerevisiae (being specially yeast saccharomyces cerevisiae CICC1415), the ratio of the living bacteria count of described subtilis (being specially subtilis CICC10076) and described product dyadic streptomycete (be specially and produce dyadic streptomycete ACCC40133) is 1:(1-30): (0.1-5): (1-50): (0.1-5): (1-50): (1-30).
In above-mentioned microbial inoculum, the mass percentage of moisture is 12-16%; The living bacteria count of described microbial inoculum is 2.8 × 10
8cfu/g-5.2 × 10
8cfu/g; The living bacteria count of described microbial inoculum is specially 2.8 × 10
8cfu/g, 3.2 × 10
8cfu/g, 4.7 × 10
8cfu/g or 5.2 × 10
8cfu/g.
Above-mentioned microbial inoculum packaging, store method are: adopt opaque plastics bag to pack, every bag of 0.5-2Kg, be placed in drying, cool place, ventilation environment preserve, preservation period 6 months.
Another object of the present invention is to provide a kind of method preparing above-mentioned microbial inoculum.
Method provided by the invention, comprises the steps:
1) by following 7 kinds of bacteria culture fluids mixing, obtain composite bacteria liquid: thermophilic fungus destroyed wire (Sporotrichumthermophile, be specially thermophilic fungus destroyed wire CICC2441), mould (the Trichoderma konigii of healthy and free from worry wood, be specially the mould CGMCC3.04001 of healthy and free from worry wood), whiterot fungi (Phanerochaete chrysosporium, be specially whiterot fungi CGMCC5.00776), aspergillus niger (Aspergillus niger, be specially aspergillus niger CGMCC3.3147), yeast saccharomyces cerevisiae (Saccharomyces cerevisiae, be specially yeast saccharomyces cerevisiae CICC1415), subtilis (Bacillus subtilis, be specially subtilis CICC10076) and produce dyadic streptomycete (Streptomyces ambofaciens, be specially and produce dyadic streptomycete ACCC40133),
Described bacteria culture fluid is for cultivating described bacterium, the bacteria culture fluid obtained;
2) by the mixing that adds water after described composite bacteria liquid access solid enlarged culturing base, obtain culture base-material, culture base-material described in fermentation culture, collect tunning, namely obtain microbial inoculum.
In aforesaid method, in step 1), the living bacteria count of described 7 kinds of bacteria culture fluids is all greater than 1 × 10
7cfu/ml;
The mass ratio of described 7 kinds of bacteria culture fluids is followed successively by: 1:(0.9-1.1): (0.9-1.1): (0.9-1.1): (0.9-1.1): (0.9-1.1): (0.9-1.1); The mass mixing such as specifically to can be;
Step 2) in, described composite bacteria liquid adds water after accessing solid enlarged culturing base by the inoculum size that quality volume percent is 4%-5% and is mixed to biodiversity percentage amounts is 55%-60%.
In aforesaid method, the living bacteria count of described 7 kinds of bacteria culture fluids respectively is 1.95-4.6 × 10
7cfu/ml, 0.87-5.83 × 10
8cfu/ml, 1.26-7.63 × 10
7cfu/ml, 1.4-9.47 × 10
8cfu/ml, 0.87-9.57 × 10
7cfu/ml, 1.52-8.8 × 10
8cfu/ml and 1.39-4.67 × 10
8cfu/ml;
The acquisition culture condition of described 7 kinds of bacteria culture fluids is: 28-30 DEG C, 200rpm cultivates 72-84h;
Step 2) in, described solid enlarged culturing base is prepared as follows: by the cassava starch dregs of particle diameter 0.425-0.85mm, and add the Semen Maydis powder of 10% in mass ratio, mixing, obtains solid enlarged culturing base;
The condition of described fermentation culture is specific as follows: 28-35 DEG C, described culture base-material stacking height is 10-14cm, every day stir 1-2 time, through 5-8 days fermentation culture, obtain tunning.
Be specially:
28 DEG C, described culture base-material stacking height is 10cm, every day stir 1 time, through 8 days fermentation culture, obtain tunning;
Or 35 DEG C, described culture base-material stacking height is 14cm, every day stir 2 times, through 5 days fermentation culture, obtain tunning;
In step 2) collection tunning after, also comprise the steps: that tunning being dried to biodiversity percentage amounts is 12-16%, obtains microbial inoculum.
Above-mentioned drying can be dried or less than 50 DEG C oven dry, adopts 45 DEG C of oven dry in an embodiment.
Above-mentioned thermophilic fungus destroyed wire (Sporotrichum thermophile, be specially thermophilic fungus destroyed wire CICC2441) bacteria culture fluid, mould (the Trichoderma konigii of healthy and free from worry wood, be specially the mould CGMCC3.04001 of healthy and free from worry wood) bacteria culture fluid, whiterot fungi (Phanerochaete chrysosporium, be specially whiterot fungi CGMCC5.00776) bacteria culture fluid, aspergillus niger (Aspergillus niger, be specially aspergillus niger CGMCC3.3147) bacteria culture fluid, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae, be specially yeast saccharomyces cerevisiae CICC1415) bacteria culture fluid, subtilis (Bacillus subtilis, be specially subtilis CICC10076) bacteria culture fluid and produce dyadic streptomycete (Streptomyces ambofaciens, being specially and producing dyadic streptomycete ACCC40133) bacteria culture fluid all can conventionally be prepared, can be specifically the method for following steps:
A, slant strains are cultivated: being inoculated in by often kind of bacterium on corresponding slant medium, is quiescent culture 24-48 hour under the condition of 26-30 DEG C in temperature; To be specially under the condition of under temperature is the condition of 26 DEG C quiescent culture 48 hours or 30 DEG C quiescent culture 24 hours;
B, liquid seeds enlarged culturing: the inoculation after the above-mentioned slant culture of picking is in corresponding liquid nutrient medium, and under the condition of 26-30 DEG C, 72-96 hour cultivated by 200rpm shaking table, obtains corresponding bacteria culture fluid; Be specially 28 DEG C, 200rpm cultivate 72h or 30 DEG C, 200rpm cultivates 84h;
Above-mentioned thermophilic fungus destroyed wire (Sporotrichum thermophile, be specially thermophilic fungus destroyed wire CICC2441) slant medium be prepared as follows: potato leaching liquid 1L, glucose 10.0g, agar 15.0g mix, and pH value is natural value;
Mould (the Trichoderma konigii of healthy and free from worry wood, be specially the mould CGMCC3.04001 of healthy and free from worry wood) and whiterot fungi (Phanerochaete chrysosporium, be specially whiterot fungi CGMCC5.00776) slant medium be all prepared as follows: potato leaching liquid 1L, glucose 20.0g, agar 15.0g mix, and pH value is natural value.
The slant medium of aspergillus niger (Aspergillus niger is specially aspergillus niger CGMCC3.3147) is prepared as follows: sucrose 30.0g, NaNO
33.0g, MgSO
47H
2o0.5g, KCl0.5g, FeSO
44H
2o0.01g, K
2hPO
41.0g, agar 15.0g, distilled water 1.0L mix, pH6.0-6.5;
The slant medium of subtilis (Bacillus subtilis is specially subtilis CICC10076) is prepared as follows: extractum carnis 10.0g, peptone 10.0g, pectin 10.0g, glucose 5.0g, NaCl3.0g, K
2hPO
41.0g, distilled water 1.0L, agar 20.0g mix, pH7.5;
The slant medium of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae is specially yeast saccharomyces cerevisiae CICC1415) is prepared as follows: brown sugar 50.0g, urea 1.0g, agar 20.0g, distilled water 1L mix, pH5.0-5.6;
The slant medium producing dyadic streptomycete (Streptomyces ambofaciens is specially and produces dyadic streptomycete ACCC40133) is prepared as follows: Zulkovsky starch 20.0g, KNO
31.0g, MgSO
47H
2o0.5g, NaCl0.5g, FeSO
44H
2o0.01g, K
2hPO
40.5g, agar 15.0g, distilled water 1.0L mix, pH7.2-7.4.
Above-mentioned potato leaching liquid is prepared as follows: get peeled potatoes 200g, be cut into small pieces, and the 1.0L that adds water boils 30min, and elimination potato ball, complements to 1.0L by filtrate.
Above-mentioned each bacterial strain liquid nutrient medium used is that the slant culture based formulas of each bacterial strain deducts agar.
Above-mentioned microbial inoculum or the microbial inoculum adopting aforesaid method to prepare also are the scope of protection of the invention at production biological organic fertilizer, compost or the application in promoting composting material to become thoroughly decomposed.
In above-mentioned application, described composting material is organic waste; Described organic waste is cellulose family organic waste; Described cellulose family organic waste by mass percentage be 75% organic cassava alcohol slag and mass percentage be that 25% filter mud forms.
Described composting material is 450-550:1 with the functional quality ratio of described microbial inoculum.
In above-mentioned application, described promotion composting material becomes thoroughly decomposed to be embodied in and promotes cellulose decomposition (be embodied in and improve cellulose decomposition rate) and/or improve humic acids content.
Experiment of the present invention proves, the invention provides a kind of composite fungus agent that cellulose family organic waste becomes thoroughly decomposed that promotes, it is the combination of multiple bacterium, is made up of the various bacteria had material decomposition functions such as Mierocrystalline celluloses, fungi, actinomycetes; This microbial inoculum viable count is high, act synergistically, act on stable, effectively can improve the speed of becoming thoroughly decomposed of organic waste, can be used for producing biological organic fertilizer, especially significantly can promote the decomposition of Mierocrystalline cellulose etc., accelerate course of fermentation; Reduce the production cost of complex micro organism fungicide simultaneously, improve the composite microbiological fertilizer using this microbial inoculum as core material and the biological organic fertilizer competitiveness of product in market.
Embodiment
The experimental technique used in following embodiment if no special instructions, is ordinary method.
Bacterial strain used in following embodiment, material, reagent etc., if no special instructions, all can obtain from commercial channels.
In following embodiment, bacteria used thereby is thermophilic fungus destroyed wire (Myceliophthora thermophila) preserving number CICC2441, healthy and free from worry wood mould (Trichoderma konigii) preserving number CGMCC3.04001, whiterot fungi (Phanerochaete chrysosporium) preserving number CGMCC5.00776, aspergillus niger (Aspergillus niger) preserving number CGMCC3.03147, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) preserving number CICC1415, subtilis (Bacillus subtilis) preserving number CICC10076, produce dyadic streptomycete (Streptomycesambofaciens) preserving number ACCC40133.
The cultivation of above-mentioned 7 kinds of bacterial strains all can conventionally be carried out, its solid slant culture base and the concrete visible summary of the invention part of liquid nutrient medium.
Solid enlarged culturing base is prepared as follows: by the cassava starch dregs of particle diameter 0.425-0.85mm, adds Semen Maydis powder, stir, obtain solid enlarged culturing base in the ratio of this cassava starch dregs quality 10%.
The preparation of embodiment 1, composite fungus agent and detection
1, slant strains is cultivated
Thermophilic fungus destroyed wire (Sporotrichum thermophile) preserving number CICC2441, healthy and free from worry wood mould (Trichoderma konigii) preserving number CGMCC3.4001, whiterot fungi (Phanerochaete chrysosporium) preserving number CGMCC5.776, aspergillus niger (Aspergillus niger) preserving number CGMCC3.03147, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) preserving number CICC1415, subtilis (Bacillus subtilis) preserving number CICC10076, producing these 7 kinds of bacterial strains of dyadic streptomycete (Streptomyces ambofaciens) preserving number ACCC40133 is inoculated on above-mentioned corresponding slant medium respectively, it is quiescent culture 48 hours under the condition of 26 DEG C in temperature.
2, liquid seeds enlarged culturing
Each bacterial strain respectively after picking slant culture is inoculated in corresponding liquid nutrient medium (1L culture medium inoculated 4-6 pin) respectively, under the rotating speed 200rpm condition of 28 DEG C, shaking table cultivates 72 hours, obtain the bacterium liquid of 7 kinds of bacterial strains respectively, the content of the bacterium in often kind of bacterium liquid is respectively 3.1 × 10
7cfu/ml, 2.1 × 10
8cfu/ml, 2.46 × 10
7cfu/ml, 4.9 × 10
8cfu/ml, 4.23 × 10
7cfu/ml, 2.03 × 10
8cfu/ml, 1.83 × 10
8cfu/ml.
3, solid expands fermentation culture
Respectively by each bacterial strain bacterium liquid in mass ratio equal proportion mix, make composite bacteria liquid;
The inoculum size of composite bacteria liquid by volume per-cent 4% is linked in solid enlarged culturing base, the stirring that adds water is adjusted to water content 55%(mass percentage), cultivate at the condition bottom fermentations of 28 DEG C, culture base-material stacking height is 10cm, stir 1 every day, terminate through 8 days fermentation culture, obtain tunning.
4, dry
Tunning is dried to moisture content 16% at 45 DEG C; Obtain composite fungus agent.
5, detect
The composite fungus agent goods gathering different depths detect, and the living bacteria count of composite fungus agent is 3.2 × 10
8cfu/g, moisture content is 15.4%.
6, pack, preserve
Adopt opaque plastics bag to pack, every bag of 0.5Kg, be placed in drying, cool place, ventilation environment preserve, preserving and detecting viable count after 6 months is 2.8 × 10
8cfu/g.
The preparation of embodiment 2, composite fungus agent and detection
1, slant strains is cultivated
Thermophilic fungus destroyed wire (Sporotrichum thermophile) preserving number CICC2441, healthy and free from worry wood mould (Trichoderma konigii) preserving number CGMCC3.4001, whiterot fungi (Phanerochaete chrysosporium) preserving number CGMCC5.776, aspergillus niger (Aspergillus niger) preserving number CGMCC3.03147, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) preserving number CICC1415, subtilis (Bacillus subtilis) preserving number CICC10076, producing these 7 kinds of bacterial strains of dyadic streptomycete (Streptomyces ambofaciens) preserving number ACCC40133 is inoculated on corresponding slant medium respectively, it is quiescent culture 24 hours under the condition of 30 DEG C in temperature.
2, liquid seeds enlarged culturing
Each bacterial strain respectively after picking slant culture is inoculated respectively (1L culture medium inoculated 4-6 pin) in corresponding liquid nutrient medium, shaking table 84 hours under the rotating speed 200rpm condition of 30 DEG C, obtain the bacterium liquid of 7 kinds of bacterial strains respectively, the content of the bacterium in often kind of bacterium liquid is respectively 3.8 × 10
7cfu/ml, 3.1 × 10
8cfu/ml, 4.0 × 10
7cfu/ml, 6.22 × 10
8cfu/ml, 5.83 × 10
7cfu/ml, 4.51 × 10
8cfu/ml and 2.65 × 10
8cfu/ml.
3, solid expands fermentation culture
Respectively by each bacterial strain bacterium liquid in mass ratio equal proportion mix, make composite bacteria liquid;
By composite bacteria liquid by the long-pending per-cent of quality volume percent 5%(composite bacteria liquid quality and solid enlarged culturing matrix) inoculum size be linked in solid enlarged culturing base, the stirring that adds water is adjusted to water content 60%(mass percentage), cultivate at the condition bottom fermentations of 35 DEG C, culture base-material stacking height is 14cm, stir 2 every day, terminate through 5 days fermentation culture, obtain tunning.
4, dry
Tunning is dried to moisture content 12% at 45 DEG C; Obtain composite fungus agent.
5, detect
The composite fungus agent goods gathering different depths detect, and the living bacteria count of composite fungus agent is 5.2 × 10
8cfu/g, moisture content is 13.6%.
6, pack, preserve
Adopt opaque plastics bag to pack, every bag of 1Kg, be placed in drying, cool place, ventilation environment preserve, preserving and detecting viable count after 6 months is 4.7 × 10
8cfu/g.
Embodiment 3, composite fungus agent are applied
Take manioc waste as the compost of main raw material, specific as follows:
Composting material forms: cassava alcohol slag (particle diameter is less than 2mm, and C/N is about 45) 75%(mass percentage) and filter mud (cassava alcohol factory waste water filter mud) 25%(mass percentage);
Making processes: add the composite fungus agent obtained by embodiment 1 or embodiment 2 by 0.2% and 0.22% of composting material quality respectively, C/N is regulated to be 30 with urea, water content is regulated to be 60%(mass percentage), stir, pile the strip that height is no more than 80cm, the wide 150-200cm in bottom.Adopt artificial turning, enter pliotherm period every 3d turning 1 time, cooldown period 5d turning 1 time, obtains fertilizer.Not add the same composting material of composite fungus agent for blank, do positive control to add 0.22% of composting material quality and the aspergillus niger of 0.2% and thermophilic fungus destroyed wire list bacterium respectively.
Above-mentioned fertilizer is carried out compost maturity effect detection, and result is as shown in table 1.
The detection method of cellulose decomposition rate:
(1) filter cloth is dried at 105 DEG C, kept dry.
(2) 1.0000g sample is placed in 150mL beaker, adds 70ml2.0mol/L HCl, put into high-pressure steam sterilizing pan 100 DEG C insulation 50min.
(3) filter after HCl process, filter residue 95% ethanol, dehydrated alcohol and acetone wash 2 times successively, filter residue and filter cloth are together dried, and being dried to constant weight, to deduct filter paper be heavily W
1.
(4) filter cloth surface residue is swept in beaker, add 10ml72%H
2sO
4, add 90ml distilled water after degraded 4h and spend the night.Next day filters, and filter residue is washed till about pH6.5, filter residue and filter cloth is together dried, and is dried to constant weight and deducts filter paper heavily for W
2.
Calculation formula is:
The detection method of humic acids content:
Manioc waste after the fermentation of becoming thoroughly decomposed of drying is ground into 40 orders, and precise about 1.000g, adds 0.2mol/LNaOH50ml.Centrifugation after constant-temperature table vibration 24h.The clear liquid separated is placed in triangular flask, adds 0.5mol/L HCl70ml.After leaving standstill 24h, then centrifugation.Remove upper strata sour water, throw out deionized water is washed till without Cl
-1detect.Then humic acid precipitation is washed in load weighted watch-glass, be placed on freeze-day with constant temperature in loft drier and, to constant weight, weigh.The black solid finally obtained is exactly humic acid (HA) particle.Calculation formula is:
In formula: W
1: humic acid weight, g;
W
0: sample weight, g.
Table 1 adds the promoter action of microbial inoculum to compost maturity
| Composting material | Cellulose decomposition rate | Humic acids content |
| Manioc waste+filter mud+0.2% composite fungus agent (embodiment 1) | 20.88％ | 10.08％ |
| Manioc waste+filter mud+0.22% composite fungus agent (embodiment 2) | 18.53％ | 9.78％ |
| Manioc waste+filter mud (blank) | 11.67％ | 8.11％ |
| Manioc waste+filter mud+0.22% black-koji mould (positive control) | 15.41％ | 7.82％ |
| Manioc waste+filter mud+0.2% thermophilic fungus destroyed wire bacterium (positive control) | 12.97％ | 7.83％ |
As can be seen from Table 1, good promoter action is had to cellulose decomposition and humic acids accumulation after adding the composite fungus agent obtained by the present invention.
Claims (8)
1. the microbial inoculum promoting composting material to become thoroughly decomposed, its activeconstituents is thermophilic fungus destroyed wire (Sporotrichumthermophile) CICC 2441, healthy and free from worry wood mould (Trichoderma konigii) CGMCC 3.04001, whiterot fungi (Phanerochaete chrysosporium) CGMCC 5.00776, aspergillus niger (Aspergillus niger) CGMCC3.3147, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) CICC1415, subtilis (Bacillussubtilis) CICC 10076 and product dyadic streptomycete (Streptomyces ambofaciens) ACCC 40133,
The ratio of the living bacteria count of described thermophilic fungus destroyed wire, mould, the described whiterot fungi of described healthy and free from worry wood, described aspergillus niger, described yeast saccharomyces cerevisiae, described subtilis and described product dyadic streptomycete is 1:(1-30): (0.1-5): (1-50): (0.1-5): (1-50): (1-30);
Effectively total viable count of described microbial inoculum is 2.8 × 10
8cfu/g or 4.7 × 10
8cfu/g.
2. microbial inoculum according to claim 1, is characterized in that: in described microbial inoculum, the mass percentage of moisture is 12-16%.
3. prepare a method for microbial inoculum according to claim 1, comprise the steps:
1) by following 7 kinds of bacteria culture fluids mixing, obtain composite bacteria liquid: thermophilic fungus destroyed wire (Sporotrichumthermophile) bacteria culture fluid, healthy and free from worry wood mould (Trichoderma konigii) bacteria culture fluid, whiterot fungi (Phanerochaete chrysosporium) bacteria culture fluid, aspergillus niger (Aspergillus niger) bacteria culture fluid, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) bacteria culture fluid, subtilis (Bacillus subtilis) bacteria culture fluid and product dyadic streptomycete (Streptomyces ambofaciens) bacteria culture fluid,
2) by the mixing that adds water after described composite bacteria liquid access solid enlarged culturing base, obtain culture base-material, culture base-material described in fermentation culture, collect tunning, namely obtain microbial inoculum;
Step 1) in, the living bacteria count of described 7 kinds of bacteria culture fluids respectively is 1.95-4.6 × 10
7cfu/ml, 0.87-5.83 × 10
8cfu/ml, 1.26-7.63 × 10
7cfu/ml, 1.4-9.47 × 10
8cfu/ml, 0.87-9.57 × 10
7cfu/ml, 1.52-8.8 × 10
8cfu/ml and 1.39-4.67 × 10
8cfu/ml;
The mass ratio of described 7 kinds of bacteria culture fluids is followed successively by: 1:(0.9-1.1): (0.9-1.1): (0.9-1.1): (0.9-1.1): (0.9-1.1): (0.9-1.1);
Described thermophilic fungus destroyed wire is thermophilic fungus destroyed wire CICC 2441, described healthy and free from worry wood mould for the mould CGMCC3.04001 of healthy and free from worry wood, described whiterot fungi be whiterot fungi CGMCC 5.00776, described aspergillus niger is aspergillus niger CGMCC 3.3147, described yeast saccharomyces cerevisiae is yeast saccharomyces cerevisiae CICC1415, described subtilis is subtilis CICC 10076 and described product dyadic streptomycete for producing dyadic streptomycete ACCC 40133;
Step 2) in, described composite bacteria liquid adds water after accessing solid enlarged culturing base by the inoculum size that quality volume percent is 4%-5% and is mixed to biodiversity per-cent is 55%-60%.
4. method according to claim 3, is characterized in that:
The mass mixings such as described 7 kinds of bacteria culture fluids;
The acquisition culture condition of described 7 kinds of bacteria culture fluids is: 28-30 DEG C, cultivate 72-84h under 200rpm condition;
Step 2) in, described solid enlarged culturing base is prepared as follows: by the cassava starch dregs of particle diameter 0.425-0.85mm, and add the Semen Maydis powder of 10% in mass ratio, mixing, obtains solid enlarged culturing base.
5. method according to claim 4, is characterized in that:
Step 2) in, described in described fermentation culture, the condition of culture base-material is as follows: 28-35 DEG C, described culture base-material stacking height is 10-14cm, every day stir 1-2 time, through 5-8 days fermentation culture, obtain tunning;
In step 2) collection tunning after, also comprise the steps: that tunning being dried to biodiversity percentage amounts is 12-16%, obtains microbial inoculum.
6. method according to claim 5, is characterized in that:
Step 2) in, described in described fermentation culture, the condition of culture base-material is as follows: 28 DEG C, described culture base-material stacking height is 10cm, every day stir 1 time, through 8 days fermentation culture, obtain tunning;
Or 35 DEG C, described culture base-material stacking height is 14cm, every day stir 2 times, through 5 days fermentation culture, obtain tunning.
7. the microbial inoculum described in claim 1 or 2 is producing biological organic fertilizer, compost or the application in promoting composting material to become thoroughly decomposed.
8. application according to claim 7, is characterized in that:
Described composting material by mass percentage be 75% cassava alcohol slag and mass percentage be that 25% filter mud forms;
Described composting material is 450-550:1 with the functional quality ratio of described microbial inoculum;
Described promotion composting material becomes thoroughly decomposed for promoting cellulose decomposition and/or improving humic acids content.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310292272.9A CN103320369B (en) | 2013-07-12 | 2013-07-12 | Composite inoculant for promoting decomposing of cellulose organic wastes, and preparation method thereof |
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|---|---|---|---|
| CN201310292272.9A CN103320369B (en) | 2013-07-12 | 2013-07-12 | Composite inoculant for promoting decomposing of cellulose organic wastes, and preparation method thereof |
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| Publication Number | Publication Date |
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| CN108587967B (en) * | 2018-04-30 | 2020-06-16 | 黄山中科新佳生物科技有限公司 | Preparation method and application of high-temperature-resistant salt-tolerant kitchen waste decomposing composite microbial agent |
| CN109055263A (en) * | 2018-08-13 | 2018-12-21 | 西南科技大学 | The antimicrobial composition and biodegrading process of degradation heavy metal accumulation plant |
| CN109679874A (en) * | 2019-01-15 | 2019-04-26 | 青岛科信新能源技术有限公司 | A method of biomass thermal liquid waste solution is handled using microbial strains |
| CN112205514B (en) * | 2020-09-29 | 2023-06-23 | 天津科技大学 | Method for producing multifunctional distiller's grains feed |
| CN113582777A (en) * | 2021-09-08 | 2021-11-02 | 重庆市风景园林科学研究院 | Fermentation inoculant and application technology thereof in garden plant waste composting |
| CN114015597A (en) * | 2021-11-04 | 2022-02-08 | 上海玖霖环保科技有限公司 | Environment-friendly biological agent for treating garden residual branch organic garbage |
| CN114058540A (en) * | 2021-11-04 | 2022-02-18 | 上海玖霖环保科技有限公司 | Environment-friendly biological agent for treating organic garbage of fruits and vegetables |
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| CN102212494B (en) * | 2011-04-12 | 2013-03-13 | 王颖 | Organic matter decomposing inoculant, and preparation method and application thereof |
| CN102276301B (en) * | 2011-06-02 | 2013-05-22 | 武汉金禾科技发展有限公司 | Straw decomposing agent and production method thereof |
| CN102178045A (en) * | 2011-06-23 | 2011-09-14 | 南开大学 | Method for producing feed by fermenting and ammoniating straws |
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