CN113582777A - Fermentation inoculant and application technology thereof in garden plant waste composting - Google Patents
Fermentation inoculant and application technology thereof in garden plant waste composting Download PDFInfo
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12N1/18—Baker's yeast; Brewer's yeast
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a fermentation inoculant and an application technology thereof in garden plant waste composting, and belongs to the technical field of fermentation inoculants. The microbial agent comprises a carrier and a compound bacterium, wherein the fermentation microbial agent comprises the following components in percentage by strain content: 50-70% of bacillus subtilis, 10-15% of bacillus amyloliquefaciens, 5-10% of saccharomyces cerevisiae and 15-25% of trichoderma. In the fermentation inoculant for garden plant waste composting, bacillus subtilis and bacillus amyloliquefaciens are bacteria, saccharomyces cerevisiae and trichoderma are fungi which are not antagonistic to each other, the effects of all strains in the compost can be fully exerted through the synergistic interaction between the fungi and the bacteria, and the effects of accelerating temperature rise and prolonging the high-temperature duration are shown; the decomposition of macromolecular organic matters is accelerated, and the nutrient content is improved; the decomposition degree of the compost is improved, the decomposition period is shortened, and the fertilizer efficiency of compost products is improved.
Description
Technical Field
The invention relates to the technical field of fermentation inoculants, in particular to a fermentation inoculants and an application technology thereof in garden plant waste composting.
Background
In recent years, the amount of landscaping wastes such as dead branches and fallen leaves, branch trimmers, lawn trimmers, weeds, seeds, residual flowers and the like is increasing, the landscaping plant wastes are the products of landscaping trimming work, and the current resource utilization mainly adopts means such as landfill and incineration, wherein the landfill needs to occupy a large amount of urban garden land and wastes land resources; incineration increases carbon dioxide emissions. Some landscaping workers often directly stack harmful diseases and pests to save cost, and bring potential safety hazards to the environment and human beings.
The microbial agent is adopted to carry out composting fermentation treatment on garden plant wastes, so that nutrient substances such as fibers, proteins and the like in the wastes can be degraded and changed into substances capable of being absorbed by plants, and meanwhile, the composting fermentation treatment meets the national industrial policies of reduction, harmlessness, ecology, resource utilization and commercialization of solid waste treatment.
Composting is the process of making organic matter into a decomposed fertilizer through the action of fermentation under the action of microorganisms, and then carrying out mineralization, humification and harmless treatment on the organic matter. During the process of decomposing organic matters by microorganisms, a large amount of available nitrogen, phosphorus and potassium compounds which can be reused by plants are generated, and humus which is an important active substance forming the soil fertility is also formed.
Because the urban garden waste is mainly composed of dead branches and fallen leaves, the chemical components of the urban garden waste are mainly lignin, cellulose and hemicellulose, the lignin, the cellulose and the hemicellulose are mutually embedded and formed, the structure of the lignin is complex and irregular, and molecules such as the cellulose are embedded in the lignin to form a natural barrier, so that the application of the garden waste in humification is limited. In the composting process, the effect of degrading lignin by using microorganisms is limited, and the composting efficiency and quality are not high.
Disclosure of Invention
The invention aims to provide a fermentation inoculant and an application technology thereof in garden plant waste composting, and aims to solve the problems of low effect and low quality of microbial composting caused by the influence of lignin in the conventional garden plant waste composting treatment.
The technical scheme for solving the technical problems is as follows:
a fermentation inoculum comprises a carrier and a compound bacterium, and comprises the following components in percentage by strain content: 50-70% of bacillus subtilis, 10-15% of bacillus amyloliquefaciens, 5-10% of saccharomyces cerevisiae and 15-25% of trichoderma.
Further, in a preferred embodiment of the present invention, the total bacteria content in the fermentation inoculum is (1.0-1.2) × 109CFU/g。
Further, in a preferred embodiment of the present invention, the preparation of the fermentation inoculum comprises the following steps:
(1) respectively and sequentially culturing bacillus subtilis, bacillus amyloliquefaciens, saccharomyces cerevisiae and trichoderma in a primary fermentation broth culture medium and a secondary fermentation broth culture medium to obtain secondary fermentation seed liquid;
(2) respectively mixing the secondary fermentation seed liquid with an adsorption carrier according to a mass ratio of 1:1, adjusting the temperature to 16-20 ℃ in an aseptic environment, and drying for 7-9 days by indoor cold air;
(3) and crushing the dried microbial inoculum, and mixing according to the percentage of the content of the strains to prepare the fermentation microbial inoculum.
Further, in a preferred embodiment of the present invention, the first-stage fermentation broth culture medium in step (1) comprises, by mass: 3g of beef extract, 5g of peptone and 1000mL of water, and the pH value is 7.2-7.4.
Further, in a preferred embodiment of the present invention, in the step (1), the secondary fermentation broth culture medium of saccharomyces cerevisiae and trichoderma comprises, by mass: 200g of potatoes, 20g of glucose or sucrose and 1000mL of water;
the secondary fermentation broth culture medium of the bacillus subtilis and the bacillus amyloliquefaciens comprises the following components in parts by mass: 10g of waste molasses, 10g of corn flour, 10g of soybean meal, 4g of calcium chloride and 1000mL of water, and the pH value is 6.8-7.2.
Further, in a preferred embodiment of the present invention, the adsorption carrier in the step (2) comprises: mixing rice bran and wheat bran according to the mass ratio of 1: 1.
Further, in a preferred embodiment of the present invention, the concentration of the bacterial strain of the dried bacterial agent in the step (2) is 5 hundred million cells/g.
The invention also provides an application technology of the fermentation inoculant in garden plant waste compost, and the application technology comprises the following steps:
(1) collecting garden plant wastes, and crushing the garden plant wastes into granular materials with the particle size of less than 2 cm;
(2) soaking the granular material in water, and mixing with fermenting agent in an amount of 2m3: (0.8-1) kg, and adjusting the carbon-nitrogen ratio to 30-40: 1, adjusting the water content to be 50-60%, piling up the mixture into a pile body with the height of 1.2-1.5 m, and then fermenting;
(3) raising the temperature of the pile body to 52-57 ℃ for 10-12 days, composting for 3-4 months, and turning the pile once every 3-5 days.
Further, in a preferred embodiment of the present invention, the garden plant waste in the step (1) comprises: one or more of dry branches and fallen leaves, branch trimmings, lawn trimmings, weeds, seeds and residual flowers.
The invention has the following beneficial effects:
1. in the fermentation inoculant, bacillus subtilis and bacillus amyloliquefaciens are bacteria, saccharomyces cerevisiae and trichoderma are fungi which are not antagonistic to each other, the functions of all strains in compost can be fully exerted through the synergistic interaction between the fungi and the bacteria, and the effects of accelerating temperature rise and prolonging the duration time of high temperature are shown; the decomposition of organic matters is accelerated, the nitrogen loss is reduced, and the nutrient content is improved; the decomposition degree of the compost is improved, the decomposition period is shortened, and the fertilizer efficiency of compost products is improved. Among them, Bacillus subtilis produces various antibiotics and enzymes, and has broad-spectrum antibacterial activity. The bacillus amyloliquefaciens is used as a bacterial strain, can effectively promote the decomposition of organic matters and produce a large amount of amino acid. The yeast contains a large amount of protein and rich nutrients, can be used by microorganisms, is an important nutritional functional bacterium, has a fermentation and decomposition effect, and can promote the value increase of various effective microorganisms in the application and replication processes of the compost composite microbial agent. In the presence of trichoderma, bacillus subtilis has higher extracellular cellulase activity, fungi has stronger cellulose degradation capacity, and the fungi can appear after the high-temperature period is finished, so that the cellulose and hemicellulose are quickly utilized to quickly propagate, the full decomposition of compost is accelerated, and the composting efficiency and the composting quality are improved.
2. According to the invention, garden plant waste is quickly humified by measures of crushing treatment, fermenting microbial inoculum, increasing carbon nitrogen content and water content and the like, so that the content of organic matters in soil is increased, and the composting efficiency and quality are improved.
3. The garden plant waste is firstly crushed into particle materials, so that the garden plant waste is easier to soak in water, the materials and the fermentation bacteria agent can be mixed more fully, and the composting period can be effectively shortened. By adjusting the carbon-nitrogen ratio and the water content, the granular materials and the fermentation bacteria agent are in the best composting condition, so that the temperature rise of the compost is accelerated, the number of days for maintaining the temperature of the compost is prolonged, and the germination index is improved. And (3) continuously fermenting for 10-12 days when the temperature of the compost reaches 52-57 ℃, shortening the composting time by 50% compared with natural composting, and completing composting in 3 months in summer and 3 months in winter.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a graph showing the temperature changes of composts of experimental example 1, which tests different microbial agents to treat dry branches and fallen leaves;
FIG. 2 is a graph showing the germination indexes of composts obtained by testing different microbial agents in test example 2 to treat fallen leaves of dead branches.
Detailed Description
The principles and features of the present invention are described below in conjunction with the embodiments and the accompanying drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
It should be noted that, the garden plant waste in the embodiment of the present invention includes: one or more of dry branches and fallen leaves, branch trimmings, lawn trimmings, weeds, seeds and residual flowers. The adsorption carrier includes: mixing rice bran and wheat bran according to the mass ratio of 1: 1.
Example 1:
the fermentation inoculum of the embodiment comprises a carrier and a compound bacterium, and comprises the following components in percentage by the content of strains: 50% of bacillus subtilis, 15% of bacillus amyloliquefaciens, 10% of saccharomyces cerevisiae and 25% of trichoderma.
The preparation of the fermentation inoculum of the embodiment comprises the following steps:
(1) respectively and sequentially culturing bacillus subtilis, bacillus amyloliquefaciens, saccharomyces cerevisiae and trichoderma in a primary fermentation broth culture medium and a secondary fermentation broth culture medium to obtain secondary fermentation seed liquid;
the first-level fermentation broth culture medium comprises the following components in percentage by mass: 3g of beef extract, 5g of peptone and 1000mL of water, and the pH value is 7.2;
the culture medium for the secondary fermentation bacteria liquid of the saccharomyces cerevisiae and the trichoderma comprises the following components in percentage by mass: 200g of potatoes, 20g of glucose or sucrose and 1000mL of water;
the secondary fermentation broth culture medium of the bacillus subtilis and the bacillus amyloliquefaciens comprises the following components in parts by mass: 10g of waste molasses, 10g of corn flour, 10g of soybean meal, 4g of calcium chloride and 1000mL of water, and the pH value is 6.8-7.2.
(2) Respectively mixing the secondary fermentation seed liquid with an adsorption carrier according to a mass ratio of 1:1, adjusting the temperature to 16 ℃ in a sterile environment, and carrying out indoor cold air drying treatment for 7 d; the strain concentration of the dried microbial inoculum is 5 hundred million bacteria/g;
(3) and crushing the dried microbial inoculum, and mixing according to the percentage of the content of the strains to prepare the fermentation microbial inoculum.
The application technology of the fermentation inoculant in garden plant waste composting comprises the following steps:
(1) collecting garden plant wastes, and crushing the garden plant wastes into granular materials with the particle size of less than 2 cm;
(2) soaking the granular material in water, and mixing with the total bacteria content of 1.0 × 109The fermentation inoculum of CFU/g is 2m3: 0.8kg of the components are mixed, and the carbon-nitrogen ratio is adjusted to be 30: 1, adjusting the water content to 50%, and piling up the mixture into a pile body with the height of 1.2m for fermentation;
(3) the temperature of the heap was raised to 52 ℃ for 10 days and the compost was allowed to stand for 3 months, during which time the heap was turned over every 3 days.
Example 2:
the fermentation inoculum of the embodiment comprises a carrier and a compound bacterium, and comprises the following components in percentage by the content of strains: 60% of bacillus subtilis, 12% of bacillus amyloliquefaciens, 8% of saccharomyces cerevisiae and 20% of trichoderma.
The preparation of the fermentation inoculum of the embodiment comprises the following steps:
(1) respectively and sequentially culturing bacillus subtilis, bacillus amyloliquefaciens, saccharomyces cerevisiae and trichoderma in a primary fermentation broth culture medium and a secondary fermentation broth culture medium to obtain secondary fermentation seed liquid;
the first-level fermentation broth culture medium comprises the following components in percentage by mass: 3g of beef extract, 5g of peptone and 1000mL of water, and the pH value is 7.3;
the culture medium for the secondary fermentation bacteria liquid of the saccharomyces cerevisiae and the trichoderma comprises the following components in percentage by mass: 200g of potatoes, 20g of glucose or sucrose and 1000mL of water;
the secondary fermentation broth culture medium of the bacillus subtilis and the bacillus amyloliquefaciens comprises the following components in parts by mass: 10g of waste molasses, 10g of corn flour, 10g of soybean meal, 4g of calcium chloride and 1000mL of water, and the pH value is 6.8-7.2.
(2) Respectively mixing the secondary fermentation seed liquid with an adsorption carrier according to a mass ratio of 1:1, adjusting the temperature to 18 ℃ in a sterile environment, and carrying out indoor cold air drying treatment for 8 d; the strain concentration of the dried microbial inoculum is 5 hundred million bacteria/g;
(3) and crushing the dried microbial inoculum, and mixing according to the percentage of the content of the strains to prepare the fermentation microbial inoculum.
The application technology of the fermentation inoculant in garden plant waste composting comprises the following steps:
(1) collecting garden plant wastes, and crushing the garden plant wastes into granular materials with the particle size of less than 2 cm;
(2) soaking the granular material in water, and mixing with the total bacteria content of 1.1 × 109The fermentation inoculum of CFU/g is 2m3: 0.9kg of the mixture is mixed, and the carbon-nitrogen ratio is adjusted to 35: 1, adjusting the water content to 55%, and piling up the mixture into a pile body with the height of 1.3m for fermentation;
(3) the temperature of the heap is raised to 55 ℃ for 11 days, and the compost is carried out for 3.5 months, during which the heap is turned every 4 days.
Example 3:
the fermentation complex bacteria agent of the embodiment comprises a carrier and a compound bacteria, and the fermentation bacteria agent comprises the following components in percentage by bacteria content: 70% of bacillus subtilis, 15% of bacillus amyloliquefaciens, 10% of saccharomyces cerevisiae and 25% of trichoderma.
The preparation of the fermentation inoculum of the embodiment comprises the following steps:
(1) respectively and sequentially culturing bacillus subtilis, bacillus amyloliquefaciens, saccharomyces cerevisiae and trichoderma in a primary fermentation broth culture medium and a secondary fermentation broth culture medium to obtain secondary fermentation seed liquid;
the first-level fermentation broth culture medium comprises the following components in percentage by mass: 3g of beef extract, 5g of peptone and 1000mL of water, and the pH value is 7.4;
the culture medium for the secondary fermentation bacteria liquid of the saccharomyces cerevisiae and the trichoderma comprises the following components in percentage by mass: 200g of potatoes, 20g of glucose or sucrose and 1000mL of water;
the secondary fermentation broth culture medium of the bacillus subtilis and the bacillus amyloliquefaciens comprises the following components in parts by mass: 10g of waste molasses, 10g of corn flour, 10g of soybean meal, 4g of calcium chloride and 1000mL of water, and the pH value is 6.8-7.2.
(2) Respectively mixing the secondary fermentation seed liquid with an adsorption carrier according to a mass ratio of 1:1, adjusting the temperature to 20 ℃ in a sterile environment, and carrying out indoor cold air drying treatment for 9 d; the strain concentration of the dried microbial inoculum is 5 hundred million bacteria/g;
(3) and crushing the dried microbial inoculum, and mixing according to the percentage of the content of the strains to prepare the fermentation microbial inoculum.
The application technology of the fermentation inoculant in garden plant waste composting comprises the following steps:
(1) collecting garden plant wastes, and crushing the garden plant wastes into granular materials with the particle size of less than 2 cm;
(2) soaking the granular material in water, and mixing with the total bacteria content of 1.2 × 109The fermentation inoculum of CFU/g is 2m3: 1kg of the raw materials are mixed, and the carbon nitrogen ratio is adjusted to be 40: 1, adjusting the water content to 60 percent, and piling up the mixture into a pile body with the height of 1.5m for fermentation;
(3) the temperature of the heap was raised to 57 ℃ for 12 days and composting was continued for 4 months, during which the heap was turned every 5 days.
Comparative example 1
The application technology of the fermentation inoculant in garden plant waste composting is the same as that in example 3, and the difference is that the same amount of Shandong Junde decomposition decomposing inoculant in the step (2) is added.
Comparative example 2
The application technology of the fermentation inoculum of the comparative example in garden plant waste composting is the same as that in example 3, and the difference is that the same amount of Beijing garden organic waste decomposing inoculant is added in the step (2).
Comparative example 3
The application technology of the fermentation inoculum of the comparative example in garden plant waste composting is the same as that in example 3, and the difference is that the fermentation inoculum is not added in the step (2).
Test example 1: temperature monitoring
The temperature of the pile of example 3 and comparative examples 1 to 3 was monitored, and the temperature was measured every two days, with the measurement position being the middle of the pile, and the change in compost temperature was measured for 1, 3, 5. cndot. 31 days by inserting a point thermometer into the pile. The results are shown in FIG. 1.
As can be seen from the figure, each heap reached 50 ℃ at 5 days of composting, the heaps containing the fermentation inoculum of example 3 and the heaps containing the Beijing cattail garden fermentation koji of comparative example 2 were maintained at 55 ℃ or higher for 4 days, and the heaps of example 3 reached 55 ℃ at 5 days, which was 2 days faster than the heaps of comparative example 2. The heap of control 2 containing Shandong Junde decomposition ripening agent was maintained above 55 deg.C for 2 days, and reached 55 deg.C on day 7 as with the heap of control 2. The results in FIG. 1 show that the temperature of the compost in bulk using the fermentation inoculum was raised faster and maintained for longer days in example 3.
Test example 2: determination of seed germination index
Taking fresh samples of the fermentation materials of the embodiment 3 and the comparison examples 1-2 to leach according to the water-fertilizer ratio of 5: 1, sucking 5mL of leaching liquor, placing the leaching liquor into culture dishes padded with filter paper, setting blank controls (distilled water), placing 10 full wheat seeds in each culture dish, culturing at the constant temperature of 20 ℃ for 24h, measuring the germination percentage and the root length, and calculating GI. GI is determined by the following formula [ (%) [ germination rate of seeds x length of seeds of compost extract)/(germination rate of seeds of distilled water x length of seeds) ]. The results are shown in FIG. 2:
as can be seen from figure 2, the germination index of the seeds of each fresh fermentation material sample can reach 70% after 20 days of composting, which is significantly higher than that of the blank control. The germination index of the fermented material in example 3 is gradually increased, the first 20 days are not influenced by the composting time, and the germination index reaches the maximum value of 70% at 20 days. The germination index of the fermented material of the comparative example 2 is slowly increased from 5d to 10d compared with that of the fermented material of the example 3, and is rapidly increased to 70% from 10d to 20d, the germination index of the fermented material of the example 1 is rapidly increased after the early growth on day 10, and the germination index of the fermented material of the comparative example 2 is still lower than that of the fermented materials of the example 3 and the comparative example 2 at 20 d.
TABLE 1 physical and chemical properties of composted products
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (9)
1. The fermentation inoculant is characterized by comprising a carrier and compound bacteria, and comprises the following components in percentage by strain content: 50-70% of bacillus subtilis, 10-15% of bacillus amyloliquefaciens, 5-10% of saccharomyces cerevisiae and 15-25% of trichoderma.
2. The fermentation inoculant according to claim 1, wherein the total inoculant content is (1.0-1.2) x 109CFU/g。
3. The fermentation inoculant according to claim 1 or 2, wherein the preparation of the fermentation inoculant comprises the following steps:
(1) respectively and sequentially culturing bacillus subtilis, bacillus amyloliquefaciens, saccharomyces cerevisiae and trichoderma in a primary fermentation broth culture medium and a secondary fermentation broth culture medium to obtain secondary fermentation seed liquid;
(2) respectively mixing the secondary fermentation seed liquid with an adsorption carrier according to a mass ratio of 1:1, adjusting the temperature to 16-20 ℃ in an aseptic environment, and drying for 7-9 days by indoor cold air;
(3) and crushing the dried microbial inoculum, and mixing according to the percentage of the content of the strains to prepare the fermentation microbial inoculum.
4. The zymophyte agent as claimed in claim 3, wherein the first-level zymophyte liquid culture medium in the step (1) comprises the following components by mass: 3g of beef extract, 5g of peptone and 1000mL of water, and the pH value is 7.2-7.4.
5. The fermentation bacteria agent of claim 3, wherein in the step (1), the secondary fermentation bacteria liquid culture medium of Saccharomyces cerevisiae and Trichoderma comprises the following components by mass: 200g of potatoes, 20g of glucose or sucrose and 1000mL of water;
the secondary fermentation broth culture medium of the bacillus subtilis and the bacillus amyloliquefaciens comprises the following components in parts by mass: 10g of waste molasses, 10g of corn flour, 10g of soybean meal, 4g of calcium chloride and 1000mL of water, and the pH value is 6.8-7.2.
6. The fermentation bacteria agent of claim 5, wherein the adsorption carrier in the step (2) comprises: mixing rice bran and wheat bran according to the mass ratio of 1: 1.
7. The fermentation bacteria agent of claim 3, wherein the bacteria concentration of the bacteria agent after drying treatment in step (2) is 5 hundred million bacteria/g.
8. The application technology of the zymophyte agent in garden plant waste compost according to any one of claims 1 to 7, characterized in that the application comprises the following steps:
(1) collecting garden plant wastes, and crushing the garden plant wastes into granular materials with the particle size of less than 2 cm;
(2) soaking the granular material in water, and mixing with fermenting agent in an amount of 2m3: (0.8-1) kg, and adjusting the carbon-nitrogen ratio to 30-40: 1, adjusting the water content to be 50-60%, piling up the mixture into a pile body with the height of 1.2-1.5 m, and then fermenting;
(3) raising the temperature of the pile body to 52-57 ℃ for 10-12 days, composting for 3-4 months, and turning the pile once every 3-5 days.
9. The application technology of the fermentation inoculant in garden plant waste composting according to claim 8, wherein the garden plant waste in the step (1) comprises: one or more of dry branches and fallen leaves, branch trimmings, lawn trimmings, weeds, seeds and residual flowers.
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| Publication number | Priority date | Publication date | Assignee | Title |
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