CN112876299A - Straw rapid degradation microbial inoculum composition and preparation method thereof - Google Patents
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
A straw rapid degradation microbial inoculum composition comprises 10-20% of a degradation bacterium composite microbial inoculum, 40-50% of peanut straws, 10-15% of peanut shells, 20-25% of fruit and vegetable residues and 20-30% of earthworm casts; the degrading bacteria composite microbial inoculum consists of ZL-2 brevibacterium frigostabile, lactobacillus acidophilus, Indian Yersinia bailii, paenibacillus mucilaginosus N6, rhodopseudomonas and flavobacterium johnsonii; the straw rapid degradation microbial inoculum composition is combined with a film-coated composting technology for use, so that the fermentation compost body can be rapidly heated, the compost fermentation efficiency can be greatly improved, the maturity of the compost is obviously improved after the fermentation is finished, the compost fermentation effect is improved, the organic matter content of the produced fertilizer is higher (55.8%), the nutrients are more comprehensive, the absorption of plants is more facilitated, the rape yield can be improved by 57.5%, and the rape quality is effectively improved.
Description
Technical Field
The invention relates to the field of resource utilization of agricultural wastes and treatment of livestock and poultry manure, in particular to a composition for preparing a straw rapid degradation microbial inoculum, a preparation method and application thereof.
Background
Agricultural waste resources such as crop straws, livestock and poultry manure and the like in China are rich, and the theoretical resource quantity of the crop straws reaches 9.84 hundred million t in 2016, wherein the crop straws of corn, rice and wheat account for 83.51 percent of the total quantity, and the theoretical production quantity of the crop straws and the livestock and poultry manure reaches 28.8 hundred million t in 2020. Although various punishment measures are issued for straw burning all over the country, researches show that most of straws are still burned every year, so that the atmospheric quality is damaged, the ecological environment is threatened greatly, and the resource waste is serious.
The crop straws contain rich nitrogen, phosphorus and potassium elements, organic matters and a small amount of trace elements, so that the nutrients are returned to the field after proper treatment, and the method is an important step for realizing the resource, harmlessness and scale production of the crop straws. The research and popularization of cheaper and efficient organic fertilizer can solve the problems to a great extent. According to the discovery, the serious consequences of soil environmental pollution, element imbalance, soil hardening and the like can be caused by using a large amount of chemical fertilizers in the traditional agricultural planting process, and because soil problems often have concealment, hysteresis and difficult treatment, the symptoms are often serious when the symptoms are shown, and the treatment of the soil needs to consume a large amount of manpower, material resources and financial resources. The organic fertilizer is used for replacing partial fertilizer, so that the current nervous soil environment can be improved, the yield of agricultural and sideline products can be increased, and the method is an efficient and environment-friendly agricultural production mode. Organic fertilizers play an increasingly important role in modern agricultural production. However, crop straws are often high in lignification degree and difficult to degrade quickly and naturally, so that how to improve the composting effect and enhance the fertilizer efficiency of organic fertilizers has great significance for protecting the ecological environment and promoting the sustainable development of agriculture. Meanwhile, how to research and develop organic fertilizers which have more comprehensive nutrition, replaceable performance and are easier to absorb by plants to replace fertilizers is also a hot problem to be solved urgently at present.
The ZL-2 cold-resistant brevibacterium has high activity in a low-temperature environment, and the rapid propagation of the brevibacterium makes the temperature of a pile rapidly rise, so that the fermentation efficiency is improved; the lactobacillus acidophilus can decompose lignin and cellulose which are not easy to decompose under normal conditions, and ferment and decompose organic matters; the flavobacterium johnsonii can degrade a plurality of macromolecular organic matters such as starch, xyloglucan, chitin and the like, can rapidly degrade the cell wall of the straw, and can improve the decomposition rate of the crop straw. The invention combines the bacteria for degrading crop straws and producing organic fertilizer by NCS intelligent film-coated composting technology of Qingdao Zhonghai environmental engineering limited company for the first time, can improve the composting speed, enables the organic fertilizer product to be more fully fermented, releases more complete nutrients and has more stable quality, and provides theoretical support and technical support for the wide application of the invention to various agricultural and forestry economic crops.
Disclosure of Invention
The invention provides a composition for preparing agricultural waste resource utilization with high efficiency and low cost, aiming at the problems of slow composting time, incomplete straw degradation and the like of the existing organic fertilizer. Specifically, the present invention includes the following.
The invention provides a straw rapid degradation microbial inoculum composition, which comprises 10-20% of a degradation microbial inoculum, 40-50% of peanut straws, 10-15% of peanut shells, 20-25% of fruit and vegetable residues and 20-30% of earthworm casts.
The composition comprises a degrading bacterium composite microbial inoculum, peanut straws, peanut shells, fruit and vegetable residues and earthworm casts; the degrading bacteria composite microbial inoculum consists of ZL-2 brevibacterium frigostabile, lactobacillus acidophilus, Indian Yersinia bailii, paenibacillus mucilaginosus N6, rhodopseudomonas and flavobacterium johnsonii.
Optionally, the strain ZL-2 Brevibacterium fritolerans comprises a ratio of the number of colonies of Lactobacillus acidophilus, Yersinia indica, Paenibacillus mucilaginosus N6, Rhodopseudomonas and Flavobacterium johnsonii of 0.2-0.5: 1: 1-1.5: 0.5-1: 1: 0.3-0.5, more preferably 0.3:1:1.5:0.7:1: 0.5.
Preferably, the peanut straw, the peanut shell, the fruit and vegetable residues and the earthworm dung are dried and crushed to the particle size of less than 1 cm.
On the other hand, the invention provides a preparation method of the straw rapid degradation microbial inoculum composition, the degradation bacteria composition is inoculated in a liquid culture medium, and when the liquid culture medium is subjected to shake culture for 60-72 hours at the temperature of 20-30 ℃ and at the speed of 120-150 r/min, the total effective viable count in the obtained liquid microbial inoculum is 4.7 multiplied by 109More than one/ml.
The liquid culture medium comprises 1-5 parts by weight of beef extract, 5-20 parts by weight of tryptone, 1-10 parts by weight of yeast extract, 5-15 parts by weight of sodium chloride and 0.5-3L of distilled water, 10-30 parts by weight of agar powder needs to be added into the solid culture medium, and the pH value of the liquid culture medium is below 7.0. Preferably, the culture medium comprises 3 parts by weight of beef extract, 10 parts by weight of tryptone, 5 parts by weight of yeast extract, 10 parts by weight of sodium chloride and 1L of distilled water, 16 parts by weight of agar powder is required to be added into the solid culture medium, and the pH value of the liquid culture medium is below 7.0.
A method for preparing an organic fertilizer by using the composition disclosed by the invention comprises the following steps:
(1) adding the composition of the straw rapid degradation microbial inoculum into a compost body during composting, adding distilled water to adjust the moisture content to be 45-60%, and preferably, the moisture content is 55%; a static strip pile film-covering composting technology is adopted, and an air supply system is added at the bottom of a pile body to ensure sufficient fermentation oxygen;
(2) and (4) drying the fermented organic fertilizer in air, granulating, packaging and warehousing.
The temperature of the treatment is as follows: fermenting for 16-25 days at 30-40 ℃ in summer; fermenting for 24-32 days at 8-18 deg.C in winter.
The invention also relates to the application of the composition in composting and preparation of commercial organic fertilizers.
In addition, the water content of the raw materials needs to be specially controlled during fermentation, the microbial activity is reduced due to too low water content, but the anaerobic fermentation is carried out on compost materials due to lack of air due to too high water content, and NH is generated4、H2S and other harmful gases can slow down the fermentation process, so that the composting time is prolonged.
According to the invention, idle crop straws and fruit and vegetable domestic garbage are fully utilized, the organic fertilizer is produced strictly according to NY525-2012 standard, and is completely converted into the fertilizer, and no by-product is generated. The NCS intelligent film-coating and fertilizer-piling technology of Qingdao Zhonghai environmental engineering Limited company is environment-friendly, odorless, simple in production process, low in cost and convenient for large-scale use;
according to the invention, the earthworm cast is added into the organic fertilizer, the earthworm cast has high organic content and rich mineral nutrients, is a natural ecological organic fertilizer, has moderate pH value and a loose structure, and has good water and fertilizer retention performance. Compared with chemical fertilizers, the earthworm cast contains nutrients which are easily absorbed by plants, such as nitrate, phosphorus, potassium, calcium, magnesium, saccharides, amino acid and the like, can promote the growth and development of the plants, and can reduce the contents of heavy metals and nitrite in fruits and vegetables, so that the quality of the fruits and the vegetables is improved. The beneficial microorganisms rich in the wormcast play an important role in accelerating the straw degradation rate and improving the organic matter content of the soil.
According to the invention, the ZL-2 hardy brevibacterium frigostolerant, the lactobacillus acidophilus-containing, the Indian Yersinia Bayer-Ribes-type, the bacillus mucilaginosus N6, the rhodopseudomonas and the flavobacterium johnsonii composite microbial inoculum are introduced into the organic fertilizer, so that the fermentation efficiency can be improved, macromolecular organic matters such as lignin, cellulose, chitin and the like which are not easy to decompose under normal conditions can be decomposed, the cell walls of the straws can be rapidly degraded, the decomposition rate of the crop straws can be increased, and the preparation speed of the organic fertilizer can be increased. The invention firstly uses the bacteria in combination with the static strip-pile film-covering composting technology to degrade crop straws and produce organic fertilizer, can improve the preparation speed of the organic fertilizer, enables the biological organic fertilizer product to be more fully fermented, releases more complete nutrients and has more stable quality, and provides theoretical support and technical support for the wide application of the invention to various agricultural and forestry economic crops.
Drawings
FIG. 1 is a schematic diagram of the effect of the degradation bacteria bio-organic fertilizer on the physicochemical properties of soil; wherein CK is a control treatment without fertilization, CR is a conventional fertilization treatment, and OF is a treatment OF the bio-organic fertilizer obtained in example 2.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that the upper and lower limits of the range, and each intervening value therebetween, is specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control. Unless otherwise indicated, "%" is percent by weight.
The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The reagents or instruments used are conventional products available from regular distributors, not indicated by the manufacturer.
Example 1
The embodiment is a preparation example of a degrading bacteria complex microbial inoculum for degrading straws, and the specific steps are as follows:
(1) composite bacterial preparation of degrading bacteria
Respectively taking ZL-2 cold-resistant Brevibacterium ([ Brevibacterium)]frigoritolerans), Lactobacillus acidophilus (Lactobacillus acidophilus), Yersinia bailii (Beijerinckia indica), Paenibacillus mucilaginosus N6(Paenibacillus mucilaginosus N6), Rhodopseudomonas sp (Rhodopseudomonas sp) and Flavobacterium johnsonii (Flavobacterium johnsonii) were inoculated into LB medium in this order to carry out the inoculation of each individual bacterium (as a control) and a combination bacterium (the ratio of the number of colonies of Lactobacillus acidophilus, Yersinia bailii, Bacillus mucilaginosus N6, Rhodopseudomonas and Flavobacterium johnsonii in 2 Brevibacterium psychrophilum is 0.2-0.5: 1: 1-1.5: 0.5: 0.3-0.5, more preferably 0.3:1:1.5:0.7: 0.5). ) Performing activation and amplification culture test, and performing shake culture at 25 ℃ and 120r/min for 24 hours; push buttonAbsorbing the activated bacteria liquid into a corresponding liquid fermentation culture medium according to the volume ratio of 1:1, and performing shake culture for 72 hours to logarithmic phase at the temperature of 25 ℃ and at the speed of 120r/min to obtain liquid bacteria seed liquid; the total effective viable count obtained by dilution plate method is 4.7 multiplied by 109More than one/ml.
(2) Test peanut straw harvest
Drying peanut straws at 105 ℃ to constant weight, crushing, and sieving with a 20-mesh sieve for later use.
(3) Test peanut Shell procurement
Drying peanut shells at 105 ℃ to constant weight, crushing, and sieving with a 20-mesh sieve for later use.
(4) Obtaining residues of tested fruits and vegetables
Drying the fruit and vegetable residues at 105 ℃ to constant weight, crushing, and sieving with a 20-mesh sieve for later use.
(5) Obtaining of earthworm cast for test
Oven drying the earthworm cast at 105 deg.C to constant weight, pulverizing, and sieving with 20 mesh sieve
(6) Degradation efficiency of composition for degrading bacterial straw
Respectively adding the composition with the total weight of 180g into a triangular flask, wherein the proportions are 40% of peanut straw, 10% of peanut shell, 20% of fruit and vegetable residue and 20% of earthworm cast, and autoclaving at 121 ℃ for 20 min. 20mLZL-2 Brevibacterium frigidum, Lactobacillus acidophilus, Yersinia bailii, Paenibacillus mucilaginosus N6, Rhodopseudomonas, Flavobacterium johnsonii and bacterial suspension of the composite bacterial agent are respectively added into each triangular flask and evenly mixed. 3 groups of strains and combinations thereof are respectively cultured for 10 days at 30 ℃ at 120r/min, the culture is centrifuged for 10min at 5000r/min, the supernatant is discarded, the mixed solution of dilute hydrochloric acid and dilute nitric acid is repeatedly used for washing until the strains are removed, the mixed solution is centrifuged for 10min at 5000r/min, the supernatant is discarded, then the washed solution is washed by distilled water, the mixed solution is centrifuged for 10min at 5000r/min, the mixed solution is dried to constant weight at 105 ℃, and the degradation effect of each single strain and the composite microbial inoculum on the organic matter combination after being cultured for 10 days at 30 ℃ is calculated. The straw degradation rates of the single bacterial agent and the composite bacterial agent are shown in table 1.
TABLE 1 straw degradation Rate for individual bacteria and combination inocula (10 days)
Example 2
The embodiment is a preparation example for preparing an organic fertilizer from livestock and poultry manure compost, and the method comprises the following steps:
(1) the experimental cow dung and the corn straws come from cooperative units of Qingdao Zhonghai environmental engineering Limited company and local farmers according to the following weight percentages of cow dung: corn stalk: mixing the microbial inoculum in a mode of 6:3:1, and uniformly stirring (the C/N ratio is 24, and the C/P ratio is 117); meanwhile, 6 groups of control experiments with only single microbial inoculum are set. Adding pure water, stirring uniformly, and adjusting the water content of the mixed material to be about 55% to obtain the composting raw material.
(2) Piling the compost raw materials into a trapezoidal strip pile with the length of 12m, the width of 5m and the height of 1.5m, covering an NCS intelligent molecular film material of Qingdao Zhonghai environmental engineering Limited company, compacting the periphery, ensuring the fermentation temperature, arranging a ventilating pipeline at the bottom, carrying out aerobic decomposition treatment, and respectively recording the compost temperature, the compost humidity and the decomposition degree index after the fermentation is finished.
The temperature of the combined microbial inoculum is raised to 61 ℃ in the 2 nd day of fermentation, the high temperature period lasts for 14 days, then the temperature is slowly reduced to 19-21 days, the temperature is stabilized at 37-40 ℃, the water content is 46 percent, the discharging standard is reached, and the germination percentage (GI) measured by sampling is 89 percent. The temperature is higher in the early stage of the treatment 1, but the maximum temperature is 53 ℃, the compost standard is not met, and the germination percentage (GI) of a sample after 21 days of treatment is 52 percent. Treatments 2 and 6 were slow to warm, reaching 50 ℃ on day 10, and the temperature remained 67 ℃ by day 21, with Germination (GI) of 72% measured by sampling 21 days without fermentation treatment. The treatment 3 and the treatment 4 reach 55 ℃ on the 5 th day of fermentation, the temperature fluctuation at the later period is not large, but the moisture is too high on the 21 st day, the upper layer of manure is thick in flavor and is not completely fermented, and the germination percentage (GI) measured by sampling for 21 days of treatment is 76%.
Because the treated materials 1-6 do not reach the standard of maturity on day 21, the materials are not discharged, after the materials are discharged and aged for 15 days after the combined microbial inoculum is treated for 21 days, the fermented materials are yellow brown, have no pungent smell, have no large straws, have a natural loose fibrous granular structure with uniform size, and the results obtained by sampling and measuring are as follows: the determination reference GB/T19524.2 of the ascarid egg death rate is that the ascarid egg death rate is more than or equal to 95 percent, the determination reference GB/T19524.1 of the faecal coliform group is that the faecal coliform group is less than or equal to 100 per g (mL); the content of organic matters is measured by a potassium dichromate volumetric method, and the measured result shows that the content of the organic matters (by dry weight) is 55.8 percent, the organic fertilizer meets the standard of organic fertilizers, and the pH value is 7.2. All indexes meet the nutritional requirements of the organic fertilizer, the successful fermentation is shown, and the semi-finished product of the organic fertilizer is obtained.
(3) Crushing the semi-finished product of the biological organic fertilizer obtained after fermentation, naturally drying until the water content is 25.12%, granulating the obtained biological organic fertilizer, packaging and warehousing the granular product without obvious mechanical impurities, uniform size and putrefactive flavor. Through detection, the indexes of the organic fertilizer are as follows: the organic matter content is 55.8 percent, and the total nutrient is (N + P)2O5+K2O) content is 9.11%, water content is 25.12%, pH value is 6.95, and quality standard of organic fertilizer NY525-2012 (organic matter is more than or equal to 40%, total nutrient is more than or equal to 5%, water content is less than or equal to 30%, and pH value is 5.5-8.5) is met; the limit indexes of 5 heavy metals in the bio-organic fertilizer product meet NY525-2012 standards: total arsenic (As) (calculated by dry basis) is less than or equal to 15mg/kg, total cadmium (Cd) (calculated by dry basis) is less than or equal to 3mg/kg, total lead (Pb) (calculated by dry basis) is less than or equal to 50mg/kg, total chromium (Cr) (calculated by dry basis) is less than or equal to 150mg/kg, and total mercury (Hg) (calculated by dry basis) is less than or equal to 2 mg/kg.
Example 3
The method is a fertilizer efficiency determination test for planting rape by using the organic fertilizer obtained in the embodiment 2, and comprises the following steps:
1. basic conditions of the test soil
The soil to be tested is brown soil 0-20cm in plough layer soil of certain farmland in Qingdao city, Shandong province, the inking region, the basic physicochemical properties of which are shown in Table 2, and the soil is sieved by a 5mm sieve for later use. Potting experiments were performed in Qingdao Zhonghai environmental engineering, Inc.
TABLE 2 basic physicochemical Properties of the soil
2. Test work: rape, variety Suzhou qing.
3. Test treatment
1) Taking the soil back to pass through a 5cm sieve, filling the soil into flowerpots with the diameter of 30cm and the height of 70cm, and subpackaging each barrel with 10kg of soil. Three treatment control groups (CK), conventional fertilization (CR) and Organic Fertilizer (OF) were set.
2) Planting rapeseeds, and carrying out normal water and fertilizer management, wherein the nitrogen content of urea is 46.2 percent and the phosphate fertilizer is diammonium phosphate (N is more than or equal to 12 percent and P is P) in other conventional fertilizers2O5Not less than 39 percent) and the potassium fertilizer is potassium sulfate.
3) After harvesting, soil is taken to measure the change of the physicochemical property of the soil and the biomass change of the rape.
Soil pH (water-soil ratio 5: 1): measured with a DMP-2pH meter (Quark Ltd, China);
total nitrogen and total nitrogen of soil: measuring by using an element analyzer;
soil quick-acting phosphorus: NaHCO 23Leaching-molybdenum-antimony colorimetric resistance;
soil quick-acting potassium: flame photometry;
soil organic matter: potassium dichromate solution weight ratio method;
rape plant height and stem thickness: measuring by a vernier caliper and a straight ruler;
rape yield: collecting the weight of each treated mature product and calculating;
4. test for influence of degradation bacterium bio-organic fertilizer on soil fertility
As can be seen from the figure 1, the conventional fertilization provides nutrients for the growth of crops, but reduces organic matters, causes soil hardening sooner or later, and is not beneficial to creating a sustainable growth environment for the growth of rapes. The application of the degrading bacteria biological organic fertilizer can improve the soil fertility, and the content of organic matters in the soil is obviously higher than that of the soil subjected to conventional fertilization by 62.2 percent compared with the contrast. The application of the degrading bacteria biological organic fertilizer can maintain the soil fertility and reduce the occurrence degree of continuous cropping obstacles of the soil.
5. Test for influence of degrading bacteria bio-organic fertilizer on yield of rape
TABLE 3 Effect test on oilseed rape yield
In Table 3, CK represents a control treatment without fertilization, CR represents a conventional fertilization treatment, and OF represents a treatment OF the bio-organic fertilizer obtained in example 2. Note: different lower case letters in the same index indicate different treatment differences are significant (P <0.05)
Example 4
The embodiment is a test for testing the influence of the degrading bacteria organic fertilizer obtained in the embodiment 2 on the quality of rape, and the method comprises the following steps:
1. basic conditions of the test soil
The soil to be tested is brown soil of 0-20cm plough layer soil of a certain cultivated land in Qingdao city, i.e. inking zone, of Shandong province, the basic physicochemical properties are shown in Table 2, and the soil is sieved by a 5mm sieve for later use. Potting experiments were performed in Qingdao Zhonghai environmental engineering, Inc.
2. Test work: rape, variety Suzhou qing.
3. Test treatment
1) Taking the soil back to pass through a 5cm sieve, filling the soil into flowerpots with the diameter of 30cm and the height of 70cm, and subpackaging each barrel with 10kg of soil. Three treatment control groups (CK), conventional fertilization (CR) and Organic Fertilizer (OF) were set.
2) Planting rapeseeds, and carrying out normal water and fertilizer management, wherein the nitrogen content of urea is 46.2 percent and the phosphate fertilizer is diammonium phosphate (N is more than or equal to 12 percent and P is P) in other conventional fertilizers2O5Not less than 39 percent) and the potassium fertilizer is potassium sulfate.
4. Rape quality test
Vc: 2, 6-dichlorophenol indophenol process
Soluble sugar: anthrone process
Nitrate salt: the nitrate reflectometer was developed by Merck, Germany.
The results are shown in Table 4:
TABLE 4 influence test of rape quality
In Table 4, CK represents a control treatment without fertilization, CR represents a conventional fertilization treatment, and OF represents a treatment OF the bio-organic fertilizer obtained in example 2.
The series of experimental data given in the embodiment show that the straw rapid degradation microbial inoculum composition can rapidly decompose straws, can greatly improve the fermentation efficiency, is combined with the NCS intelligent film-coated composting technology of Qingdao Zhonghai environmental engineering Limited company, has higher organic matter content (55.8%) of the fertilizer, more comprehensive nutrients, is more beneficial to the absorption of plants, can improve the yield of rape by 57.5%, and effectively improves the quality of the rape.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The specification and examples are exemplary only.
Claims (8)
1. A straw rapid degradation microbial inoculum composition is characterized by comprising 10-20% of a degradation bacterium composite microbial inoculum, 40-50% of peanut straws, 10-15% of peanut shells, 20-25% of fruit and vegetable residues and 20-30% of earthworm casts; the degrading bacteria composite microbial inoculum consists of ZL-2 brevibacterium frigostabile, lactobacillus acidophilus, Indian Yersinia bailii, paenibacillus mucilaginosus N6, rhodopseudomonas and flavobacterium johnsonii.
2. The composition as claimed in claim 1, wherein the peanut straw, peanut shell, fruit and vegetable residue and earthworm cast are dried and pulverized to a particle size of less than 1 cm.
3. The composition of claim 1, wherein the ratio of the number of colonies of ZL-2 brevibacterium frigidum, lactobacillus acidophilus, yersinia indica, paenibacillus mucilaginosus N6, rhodopseudomonas and flavobacterium johnsonii is 0.2-0.5: 1: 1-1.5: 0.5-1: 1: 0.3-0.5.
4. A combination according to any one of claims 1 to 3The preparation method of the product comprises the step of inoculating the degrading bacteria combination into a liquid culture medium, and performing shake culture for 60-72 hours at the temperature of 20-30 ℃ and the speed of 120-150 r/min to obtain the liquid microbial inoculum with the total effective viable count of 4.7 multiplied by 109More than one/ml.
5. The method according to claim 4, wherein the liquid medium comprises 1 to 5 parts by weight of beef extract, 5 to 20 parts by weight of tryptone, 1 to 10 parts by weight of yeast extract, 5 to 15 parts by weight of sodium chloride, and 0.5 to 3L of distilled water, and further comprises 10 to 30 parts by weight of agar powder in the solid medium, and the pH of the liquid medium is 7.0 or less.
6. A method for preparing organic fertilizer by using the composition as claimed in any one of claims 1 to 3, comprising the following steps:
(1) adding distilled water into the composition of the straw rapid degradation microbial inoculum to adjust the water content to be 45-60% during composting, adopting NCS intelligent film coating composting technology of Qingdao Zhonghai environmental engineering Limited company, and adding an air supply system at the bottom of a compost body to ensure sufficient fermentation oxygen;
(2) and (4) aging, air-drying, granulating, packaging and warehousing the fermented organic fertilizer.
7. The method of claim 6, wherein the temperature of the treatment is: fermenting for 16-25 days at 30-40 ℃ in summer; fermenting for 24-32 days at 8-18 deg.C in winter.
8. Use of the composition according to any one of claims 1 to 4 in the preparation of commercial organic fertilizers.
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Application publication date: 20210601 |