CN103305436A - Spirulina culture medium and application thereof - Google Patents
Spirulina culture medium and application thereof Download PDFInfo
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- CN103305436A CN103305436A CN2013101238296A CN201310123829A CN103305436A CN 103305436 A CN103305436 A CN 103305436A CN 2013101238296 A CN2013101238296 A CN 2013101238296A CN 201310123829 A CN201310123829 A CN 201310123829A CN 103305436 A CN103305436 A CN 103305436A
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- spirulina
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Abstract
The invention relates to a spirulina culture medium. Underground brine in a coastal region is utilized as a basic water body, the underground brine contains a large amount of inorganic salt, the underground brine is mixed with running water in proportion, and then missing components are added according to a basic formula, so that the formed new culture medium can completely meet growth requirement of spirulina, amount of the added inorganic salt can be greatly reduced, the spirulina can grow stably, and yield is increased.
Description
Technical field
The present invention relates to the marine organisms field, be specifically related to a kind of SPIRULINA CULTIVATION base.
Background technology
Spirulina is a kind of blue photoautotrophy fine algae, belongs to Cyanophyta, Oscillariaceae, Spirullina.The protein content of spirulina is up to more than 60～70%, ideal protein for Food and Argriculture OrganizationFAO (FAD) recommendation, contain the amino acid that eight kinds of needed by human but can not self synthesize, comprise Methionin, Threonine, methionine(Met), Isoleucine, leucine, tryptophane, phenylalanine, α-amino-isovaleric acid, the human intaking amount that its content in spirulina is recommended near FAD tissue very much can reasonable supplement and fully absorb.Spirulina also contains abundant VITAMIN, polysaccharides, lipoid cpd, carotene, chlorophyll, mineral substance and Phycocyanins, C-etc. in addition, and the cell wall structure fiber of natural spiral seaweed is few, the utilization so its nutritive ingredient memory is absorbed by the body.The development at home of spirulina breeding industry is growing steadily, and will become the object of giving priority to of health products trade.
Present SPIRULINA CULTIVATION base mostly grows up on Zarrouk prescription basis, need in process of production in water, to add a large amount of inorganic salt compositions, and the cultivation water body of spirulina needs periodic replacement, all can to a large amount of brine waste of discharging in the natural water area, cause environmental pollution after every batch cultivation finishes like this.
Summary of the invention
The present invention proposes a kind of novel SPIRULINA CULTIVATION base, not only can greatly reduce the quantity of throwing in inorganic salt, and growth of spirulina platensis is stable, output increased.
Substratum of the present invention utilizes the subsurface brine of coastland as basic water body, contain a large amount of inorganic salts in the bittern, with it with after tap water proportionally mixes, add the composition that lacks according to basic components again, the new substratum that forms can satisfy the needs of growth of spirulina platensis fully, and output increased.
Subsurface brine composition analysis table
Unit: (grams per liter)
| ? | pH | Cl + | K + | Ca 2+ | Na + | Mg 2+ | SO 4 2- | HCO 3 - |
| 1 | 7.7 | 20.24 | 0.905 | 0.136 | 20.37 | 1.47 | 3.18 | 0.393 |
| 2 | 7.9 | 21.91 | 1.144 | 0.119 | 19.11 | 1.16 | 3.04 | 0.392 |
| 3 | 7.9 | 20.8 | 1.324 | 0.187 | 20.6 | 1.61 | 3.53 | 0.321 |
Zarrouk system component table
Unit: (grams per liter)
| ? | pH | Cl + | K + | Ca 2+ | Na + | Mg 2+ | SO 4 2- | HCO 3 - |
| 1 | 9.0 | 0.62 | 0.68 | 0.02 | 5.61 | 0.1 | 0.56 | 13.07 |
Find through contrast, the required basic nutritive element of growth of spirulina platensis all contains and rich content in bittern, except HCO
3 -In addition, much larger than the concentration of growth of spirulina platensis demand, so when the preparation novel culture medium, need to add a large amount of NaHCO
3, so not only replenished most important carbon source in the growth of spirulina platensis process, and the pH value of solution can rise to about 9.0 after adding, and meets the requirement of classical prescription.
In order to reduce the nutritive ingredient loss of spirulina in the course of processing, the present invention proposes a kind of new spirulina drying means, can effectively preserve the nutritive ingredient in the spirulina.
Embodiment
1, gets 200L bittern, with 5 times of pure water dilutions, then add NaHCO
318g, the novel culture medium main component that is mixed with is listed as follows:
Unit: (grams per liter)
| ? | pH | Cl + | K + | Ca 2+ | Na + | Mg 2+ | SO 4 2- | HCO 3 - |
| 1 | 9.0 | 4.16 | 0.24 | 0.224 | 7.47 | 0.24 | 0.6 | 13 |
2, get 8 of large-scale incubators (length * wide * height=240cm * 120cm * 60cm), get respectively the 4 parts of (novel culture medium of the present invention of length * wide * height=240cm * 120cm * 40cm) and 4 parts (Zarrouk substratum of length * wide * height=240cm * 120cm * 40cm), add identical spirulina plalensis, under identical condition, carry out long-term culture experiment, the output of getting optimal growth condition next month compares, and the result is as follows:
Every square of face water body yield comparison (monthly output kg):
Can find out through contrast, adopt the more original substratum output increased of yield of spirulina of new substratum obvious, on average improve more than 20%.Illustrate that novel culture medium has promoter action to the growth of spirulina.
3, in actual production process, in substratum, pour CO if adopt
2Mode replace NaHCO
3, effect can be better, and need hardly to add any inorganic salt again.
(length * wide * height=240cm * 120cm * 60cm) gets respectively 4 parts of (novel culture medium (NaHCO of length * wide * height=240cm * 120cm * 40cm) to get 8 of large-scale incubators
3) and 4 parts (CO is executed in the punching of length * wide * height=240cm * 120cm * 40cm)
2Novel culture medium, add identical spirulina plalensis, under identical condition, carry out long-term culture experiment, the output of getting optimal growth condition next month compares, the result is as follows:
Every square of face water body yield comparison (monthly output kg):
Test-results shows, rushes and executes CO
2Can satisfy equally in the growth of spirulina platensis process the demand of carbon source, output can not be affected.
So novel substratum has not only greatly reduced the input of inorganic salt in the process of culturing spirulina, alleviated the environmental stress in the breeding process, and spirulina has been had obvious production-increasing function, so have obvious advance with respect to present traditional substratum.
Claims (6)
1. a SPIRULINA CULTIVATION base is characterized in that, utilizes the subsurface brine of coastland as basic water body, contains a large amount of inorganic salts in the bittern, and it with after tap water proportionally mixes, is added the composition that lacks according to basic components again.
2. SPIRULINA CULTIVATION base according to claim 1 when preparation, need to be added NaHCO
3
3. SPIRULINA CULTIVATION base according to claim 1, when preparation, every 1000L substratum is added 18gNaHCO
3
4. SPIRULINA CULTIVATION base according to claim 1 when preparation, need to pour CO
2
5. SPIRULINA CULTIVATION base according to claim 1 pours CO when preparation
2To pH=9.0.
6. the application of SPIRULINA CULTIVATION base in spirulina breeding is characterized in that, adopts this substratum can satisfy the needs of growth of spirulina platensis, greatly reduce the quantity of throwing in inorganic salt, and growth of spirulina platensis is stable, output increased.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101238296A CN103305436A (en) | 2013-04-11 | 2013-04-11 | Spirulina culture medium and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101238296A CN103305436A (en) | 2013-04-11 | 2013-04-11 | Spirulina culture medium and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103305436A true CN103305436A (en) | 2013-09-18 |
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ID=49131158
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013101238296A Pending CN103305436A (en) | 2013-04-11 | 2013-04-11 | Spirulina culture medium and application thereof |
Country Status (1)
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104611265B (en) * | 2015-02-03 | 2017-10-03 | 宁波浮田生物技术有限公司 | A kind of Spirulina culture medium |
| CN108239605A (en) * | 2016-12-24 | 2018-07-03 | 云南爱尔发生物技术股份有限公司 | A kind of method of well mine salt brine culture Phaeodactylum tricornutum |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1594540A (en) * | 2004-02-25 | 2005-03-16 | 张德智 | Method for making spirulina alga culture solution |
| CN1807571A (en) * | 2005-11-28 | 2006-07-26 | 寿光富康制药有限公司 | Method for cultivating spirulina |
| CN102533527A (en) * | 2011-12-18 | 2012-07-04 | 鄂托克旗隆诚化工有限责任公司 | Process and system for preparing spirulina culture solution from low-concentration alkaline lake brine |
-
2013
- 2013-04-11 CN CN2013101238296A patent/CN103305436A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1594540A (en) * | 2004-02-25 | 2005-03-16 | 张德智 | Method for making spirulina alga culture solution |
| CN1807571A (en) * | 2005-11-28 | 2006-07-26 | 寿光富康制药有限公司 | Method for cultivating spirulina |
| CN102533527A (en) * | 2011-12-18 | 2012-07-04 | 鄂托克旗隆诚化工有限责任公司 | Process and system for preparing spirulina culture solution from low-concentration alkaline lake brine |
Non-Patent Citations (2)
| Title |
|---|
| 张学成等: "钝顶螺旋藻营养生理的研究I:钝顶螺旋藻对无机碳的吸收利用", 《青岛海洋大学学报》 * |
| 张峰等: "耦合二氧化碳减排的微藻产业化培养技术", 《微生物学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104611265B (en) * | 2015-02-03 | 2017-10-03 | 宁波浮田生物技术有限公司 | A kind of Spirulina culture medium |
| CN108239605A (en) * | 2016-12-24 | 2018-07-03 | 云南爱尔发生物技术股份有限公司 | A kind of method of well mine salt brine culture Phaeodactylum tricornutum |
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Application publication date: 20130918 |