CN1594540A - Method for making spirulina alga culture solution - Google Patents
Method for making spirulina alga culture solution Download PDFInfo
- Publication number
- CN1594540A CN1594540A CN 200410014154 CN200410014154A CN1594540A CN 1594540 A CN1594540 A CN 1594540A CN 200410014154 CN200410014154 CN 200410014154 CN 200410014154 A CN200410014154 A CN 200410014154A CN 1594540 A CN1594540 A CN 1594540A
- Authority
- CN
- China
- Prior art keywords
- solution
- bittern
- spirulina
- urao
- content
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000016425 Arthrospira platensis Nutrition 0.000 title claims abstract description 33
- 240000002900 Arthrospira platensis Species 0.000 title claims abstract description 33
- 229940082787 spirulina Drugs 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims description 16
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 28
- 241001131796 Botaurus stellaris Species 0.000 claims abstract description 25
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 16
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 14
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 14
- 239000011591 potassium Substances 0.000 claims abstract description 14
- 239000000463 material Substances 0.000 claims abstract description 6
- WCTAGTRAWPDFQO-UHFFFAOYSA-K trisodium;hydrogen carbonate;carbonate Chemical compound [Na+].[Na+].[Na+].OC([O-])=O.[O-]C([O-])=O WCTAGTRAWPDFQO-UHFFFAOYSA-K 0.000 claims description 21
- 229910052698 phosphorus Inorganic materials 0.000 claims description 12
- 239000011574 phosphorus Substances 0.000 claims description 12
- 150000001875 compounds Chemical class 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 abstract description 7
- 238000002360 preparation method Methods 0.000 abstract description 4
- 239000002253 acid Substances 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 abstract 3
- 241001625808 Trona Species 0.000 abstract 3
- 239000003337 fertilizer Substances 0.000 abstract 1
- 238000002798 spectrophotometry method Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 20
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 239000000203 mixture Substances 0.000 description 8
- 239000011780 sodium chloride Substances 0.000 description 5
- 238000003756 stirring Methods 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- 241000195493 Cryptophyta Species 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000003513 alkali Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- VZCCETWTMQHEPK-UHFFFAOYSA-N gamma-Linolensaeure Natural products CCCCCC=CCC=CCC=CCCCCC(O)=O VZCCETWTMQHEPK-UHFFFAOYSA-N 0.000 description 1
- VZCCETWTMQHEPK-QNEBEIHSSA-N gamma-linolenic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/CCCCC(O)=O VZCCETWTMQHEPK-QNEBEIHSSA-N 0.000 description 1
- 235000020664 gamma-linolenic acid Nutrition 0.000 description 1
- 229960002733 gamolenic acid Drugs 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a preparation method of spirulina culture solution, which comprises the steps : using natural trona bittern as basic material, determining natural trona bittern of nitrogen, phosphor, potassium content, employing spectrophotometric method or colorimetric estimation, then adding into HNO#-[3]solution, HCl solution, K#-[2]SO#-[4] and H#-[3]PO#-[4]solution to make containing 0.4-0.8g/L nitrogen, 0.07-0.1g/L phosphor, 0.3-1g/L potassium, adjusting PH to 8.5-10 using acid. The invention lowers the breeding cost dramatically due to the main fertilizer natural trona bittern and keeps the original nutrients of spirulina.
Description
Technical field
The present invention relates to the prescription of the nutrient solution of a kind of phycophyta, say the compound method that the present invention relates to spirulina medium exactly.Belong to the algal culture technical field.
Background technology
Spirullina is in a genus of blue algae door hormogon[ium order Oscillariaceae, spirulina is that the protein content of finding so far is the highest, can reach 55%～70%, proteinic amino acid is formed excellent uniformly algae, in addition, also contain unsaturated fatty acidss such as multivitamin, gamma linolenic acid, various trace elements and polysaccharides.Spirulina not only has very high nutritive value, and has excellent effects such as health care.Its market requirement day by day enlarges, and has therefore promoted the development of spirulina breeding industry.
Because spirulina is to having relatively high expectations of survival and reproduction condition on every side, so the preparation of spirulina medium is to culture one of spirulina key of success technology all the time.According to applicant's understanding, the prescription of the nutrient solution that the spirulina of home and abroad breed at present is used all is to adopt French ZARROUKShi prescription or similar modified version prescription, according to said method adopts corresponding following chemical one by one, roughly prepares by following mixed again:
Unit: g/L
NaHCO
3 8～16.8
NaNO
3 2.5
NaCl 1
K
2HPO
4 0.5
K
2SO
4 0.5
MgSO
4 0.2
CaCl
2 0.1
FeSO
4.7H
2O 0.08
pH 8.5～10
Because the procurement price of above-mentioned chemical is higher, therefore for a long time, the production cost of spirulina can't reduce, and causes the spirulina price high always, is difficult to allow consumers in general accept.
Summary of the invention
The objective of the invention is:, propose a kind of compound method that can be significantly reduced to this spirulina medium, thereby reduce the spirulina breeding cost by a relatively large margin at the shortcoming that above prior art exists.
The applicant chances in secular breed spirulina practice: contain NaHCO in the urao bittern usually
31～150g/L, NaCl 10～80g/L, NaCO
31～150g/L, KCl0.5～10g/L, SO
4 2-0.5～20g/L, Ca
2+0.5～10g/L, Mg
2+0.5～10g/L, its composition and spirulina medium are very close.Through investigation in many ways, experiment repeatedly, the applicant can be mixed with the spirulina medium that share with the very cheap urao bittern of alkali bittern, especially cost through suitable modulation.Specifically, SPIRULINA CULTIVATION liquid making method of the present invention may further comprise the steps:
(1) gets urao bittern as the preparation bed material;
(2) content of nitrogen, phosphorus, potassium in the mensuration urao bittern, measuring method can be spectroscopic analysis or colorimetric method;
(3) then to wherein adding HNO
3Solution, K
2SO
4And H
3PO
4Solution, and dilution makes that nitrogen content is 0.4～0.8g/L in the gained solution, phosphorus content is 0.07～0.1g/L, potassium content is 0.3-1g/L;
(4) PH with acid-conditioning solution is between 8.5～10, get final product spirulina medium.
The compound method of a kind of spirulina medium of the present invention can also take following steps further to realize:
The compound method of aforesaid a kind of spirulina medium is characterized in that wherein said HNO
3Solution is 98%, HCl solution 36.5%, K
2SO
4And H
3PO
4Solution is 95%.
The compound method of aforesaid a kind of spirulina medium is characterized in that wherein said urao bittern is to come from being stored in the face of land or subterranean bittern or being clarified after being dissolved in water and got by solid-state urao of natural generation.
The present invention takes conventional method to carry out direct mixed preparing to get final product.Because of various compositions in the urao bittern change along with temperature or underground mineralization degree are different thereupon, so add HNO in the different urao bittern
3Solution, HCl solution and K
2SO
4Ratio also is not quite similar.Need by the main nutrient composition concentration in the mensuration urao bittern, and then determine that institute adds auxiliary material HNO
3Solution, HCl solution and K
2SO
4Ratio.
Because the urao bittern price that the present invention adopts is extremely low, so significantly reduced the preparation cost of spirulina medium.Applicant of the present invention in addition also uses acid to prepare the nutrient solution of spirulina, prepares the imagination that nutritive medium had both exceeded people with acid, and these sour prices are very cheap again simultaneously, thereby has further reduced the production cost of spirulina.The spirulina that experiment showed, breed has better quality, can keep the various effective constituents of spirulina.
Embodiment:
Embodiment 1
Get a kind of urao bittern and measure the effective content of wherein each kind of composition, and the effective content of nitrogen, phosphorus, potassium, following data can be got: unit: g/L
NaHCO
3 5
NaCl 20
NaCO
3 140
KCl 10
SO
4 2- 20
Ca
2+ 5
Mg
2+ 5
Add following ingredients again after urao bittern can be diluted to 1/18th by underground water or tap water and stir well:
H
3PO
4Concentration is that 95% ratio is: 0.19 unit/ml/L
HNO
3Concentration is that 98% ratio is: 1.5 unit/ml/L
HCl concentration is that 36.5% ratio is: 2 unit/ml/L
K
2SO
4 0.5g/L
Make wherein that nitrogen content reaches 0.4g/L, phosphorus content is 0.08g/L, and potassium content reaches 0.44g/L;
Embodiment 2
Get a kind of urao bittern and measure the effective content of wherein each kind of composition, and the effective content of nitrogen, phosphorus, potassium, following data can be got: unit: g/L
NaHCO
3 80
NaCl 40
NaCO
3 80
KCl 10
SO
4 2- 10
Ca
2+ 5
Mg
2+5 urao bittern can be diluted to 20 fens by underground water or tap water
One of after add following other composition again and stir well:
H
3PO
4Concentration is that 95% ratio is: 0.19 unit/ml/L
HNO
3Concentration is that 98% ratio is: 1.5 unit/ml/L
HCl concentration is that 36.5% ratio is: 2 unit/ml/L
K
2SO
4 0.5g/L
Make wherein that nitrogen content reaches 0.41g/L, phosphorus content is 0.08g/L, and potassium content reaches 0.44g/L;
Embodiment 3
Get a kind of urao bittern and measure the effective content of wherein each kind of composition, and the effective content of nitrogen, phosphorus, potassium, following data: g/L can be got
NaHCO
3 140
NaCl 70
NaCO
3 10
KCl 5
SO
4 2- 5
Ca
2+ 5
Mg
2+ 10
After can being diluted to 1/19th by underground water or tap water, adds down again in urao bittern
Be listed as other composition and stir well:
H
3PO
4Concentration is that 95% ratio is: 0.19 unit/ml/L
HNO
3Concentration is that 98% ratio is: 1.5 unit/ml/L
K
2SO
4 0.7g/L
Make wherein that nitrogen content reaches 0.41g/L approximately, phosphorus content reaches 0.08g/L approximately, and potassium content reaches 0.44 g/L approximately;
The various nutritions of urao bittern in the foregoing description can be by adding K fixedly the time
2SO
4, H
3PO
4Solution, HNO
3Solution H Cl solution waits the N-P-K content regulated wherein and the PH of solution, and after nutrient solution prepared, field management was the same with traditional cultural method, needs often to measure wherein N-P-K content and pH value simultaneously.The content of the nitrogen among the above embodiment in institute's prepared culture, phosphorus, potassium is best state, if the content of the nitrogen in the gained nutrient solution, phosphorus, potassium is other numerical value, but still within the scope of aforementioned regulation, then is still feasible.
Except that above embodiment, all employings are equal to the technical scheme that method obtained of replacement or equivalent transformation, all drop within protection scope of the present invention.
Claims (4)
1, a kind of compound method of spirulina medium is characterized in that it comprises the following steps:
(1) gets urao bittern as the dosing bed material;
(2) content of nitrogen, phosphorus, potassium in the mensuration bed material;
(3) in bed material, add HNO
3Solution, K
2SO
4And H
3PO
4Solution, and make that nitrogen content is 0.4～0.8g/L in the gained solution, phosphorus content is 0.07～0.1g/L, potassium content is 0.3-1g/L;
(4) PH with acid-conditioning solution is between 8.5～10.
2, the compound method of spirulina medium according to claim 1 is characterized in that described HNO
3Strength of solution is 98%, K
2SO
4And H
3PO
4Strength of solution is 95%.
3, the compound method of spirulina medium according to claim 1, it is characterized in that described urao bittern be come from natural generation be stored in the face of land or subterranean bittern.
4, the compound method of spirulina medium according to claim 1 is characterized in that described urao bittern clarified and got by solid-state urao after being dissolved in water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410014154 CN1594540A (en) | 2004-02-25 | 2004-02-25 | Method for making spirulina alga culture solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410014154 CN1594540A (en) | 2004-02-25 | 2004-02-25 | Method for making spirulina alga culture solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1594540A true CN1594540A (en) | 2005-03-16 |
Family
ID=34663027
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410014154 Pending CN1594540A (en) | 2004-02-25 | 2004-02-25 | Method for making spirulina alga culture solution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1594540A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101463322B (en) * | 2009-01-07 | 2011-05-18 | 福清市新大泽螺旋藻有限公司 | Method for cultivating large area organic Spirulina |
| CN103305436A (en) * | 2013-04-11 | 2013-09-18 | 寿光富康制药有限公司 | Spirulina culture medium and application thereof |
| CN105754914A (en) * | 2016-05-18 | 2016-07-13 | 青岛农业大学 | Culture solution for culture of seawater spirulina |
| CN107198038A (en) * | 2017-05-24 | 2017-09-26 | 甘肃凯源生物技术开发中心 | The feed of cultivation health pig prepared by chlorella powder and spirulina powder composition and said composition |
| CN108239605A (en) * | 2016-12-24 | 2018-07-03 | 云南爱尔发生物技术股份有限公司 | A kind of method of well mine salt brine culture Phaeodactylum tricornutum |
-
2004
- 2004-02-25 CN CN 200410014154 patent/CN1594540A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101463322B (en) * | 2009-01-07 | 2011-05-18 | 福清市新大泽螺旋藻有限公司 | Method for cultivating large area organic Spirulina |
| CN103305436A (en) * | 2013-04-11 | 2013-09-18 | 寿光富康制药有限公司 | Spirulina culture medium and application thereof |
| CN105754914A (en) * | 2016-05-18 | 2016-07-13 | 青岛农业大学 | Culture solution for culture of seawater spirulina |
| CN108239605A (en) * | 2016-12-24 | 2018-07-03 | 云南爱尔发生物技术股份有限公司 | A kind of method of well mine salt brine culture Phaeodactylum tricornutum |
| CN107198038A (en) * | 2017-05-24 | 2017-09-26 | 甘肃凯源生物技术开发中心 | The feed of cultivation health pig prepared by chlorella powder and spirulina powder composition and said composition |
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| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |