CN103340304B - Anti-stress compound feed for macrobrachium nipponense - Google Patents
Anti-stress compound feed for macrobrachium nipponense Download PDFInfo
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- CN103340304B CN103340304B CN201310244980.5A CN201310244980A CN103340304B CN 103340304 B CN103340304 B CN 103340304B CN 201310244980 A CN201310244980 A CN 201310244980A CN 103340304 B CN103340304 B CN 103340304B
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- stress
- macrobrachium nipponensis
- mixed feed
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Abstract
The invention discloses an anti-stress compound feed for macrobrachium nipponense. The anti-stress compound feed comprises, by mass, 36.0%-40.0% of crude protein, 6%-8% of crude fat, 7%-9% of ash, 15%-25% of carbohydrates, 1%-2% of an anti-stress additive and 7%-9% of water. The compound feed is simple in preparation and balanced in nutrients, and can meet the nutritional requirements of the macrobrachium nipponense, improve the quality of aquaculture products, improve resilience of the macrobrachium nipponense and reduce deterioration of water quality during a culture process.
Description
Technical field
The present invention relates to that a kind of Macrobrachium nipponensis is anti-stress mixed feed.
Background technology
Macrobrachium nipponensis, is commonly called as freshwater shrimp, is the economic shrimps of the important freshwater aquiculture of China.Its growth is fast, and individual large, breeding is fast, and vitality is strong, is distributed widely in China's freshwater lake.The features such as that Macrobrachium nipponensis has is partially carnivorous, growth fast, disease is few, Macrobrachium nipponensis meat is good in addition, and economic worth is high, so market prospects are good.In recent years, the ground such as China's Jiangsu-zhejiang Shanghai Area have risen the large-scale cultivation of Macrobrachium nipponensis, and progressively expand to other aquaculture area; at present; more than 20 ten thousand tons of whole nation Macrobrachium nipponensis annual productions, the output value surpasses 10,000,000,000 yuan, has become one of approach of China's fishery synergy, fisherman's increased income.The Macrobrachium nipponensis market price is stable, remains on more than 50 yuan/kilogram always, and culture benefit is considerable, especially the in the situation that of the depression of current fish market, and the freshwater aquiculture new varieties of the more suitable selection of raiser.
Yet, current rarer special Macrobrachium nipponensis mixed feed on the market, market mixed feed is adopted other product seed shrimp crab formula feeds such as Penaeus Vannmei, river crab more.Although this class feed can provide nutriment, but the nutrient composition content such as crude protein, aliphatic acid, amino acid, carbohydrate, vitamin, mineral matter and proportioning can not meet the demand of Macrobrachium nipponensis growth to this several nutrient components completely in its feed, and likely because feed coefficient is crossed the problem such as high and caused the situations such as the various wastings of resources, environmental pollution.The corruption of the high temperature time cultivation a large amount of accumulations of place bottom (not digesting feed and excreta) is decomposed; thereby cause water bottom anoxic and ammonia nitrogen and content of nitrite to increase; even cause emergency anoxic in water to cause " the general pool " accident; in addition Macrobrachium nipponensis lower oxygen concentration resistance not; therefore; seek balanced in nutrition, alleviate stress coerce, ecological, environmental protective, Macrobrachium nipponensis special feed formula safely and efficiently more, to the healthy aquaculture of Macrobrachium nipponensis, improve product quality, improve culture benefit, preserving the ecological environment all has great importance.
Resist and stress comprise astaxanthin, glutamine, betaine and allicin by additive, astaxanthin has stronger non-oxidizability, can maintain efficiently the dynamic equilibrium of interior free yl; Glutamine is γ-Carboxylamide compound of L-glutamic acid, is one of amino acid the abundantest in body fluid, can maintain phagocytosis and the lymphocytic propagation of acid-base balance, increase cell; Betaine also can be used as a kind of living things system Premeabilisation of cells protective agent, improves biological cell arid, high temperature, high salt and height are oozed to the tolerance under environment, has the effect of stabilized enzyme activity and large biological molecule function; A kind of organosulfur compound extracting in the bulb (garlic) of allicin Shi Cong green onion section allium garlic; can strengthen the activity that increases liver cell GSH-PX activity s-transferase; increase the combination function of detoxification of liver, thereby liver and even whole body are shielded.
So far, more existing about astaxanthin, glutamine, betaine and allicin in aquaculture, have alleviation stress the report of effect, but about the compatibility of astaxanthin, glutamine, betaine and allicin, use, have not been reported.Therefore developing that a kind of Macrobrachium nipponensis is anti-stress mixed feed, improves the anti-stress ability of Macrobrachium nipponensis, and the sustainable development of prawn crab healthy aquaculture is significant.
Summary of the invention
The defect that the object of the invention is to exist for prior art provides a kind of preparation simple, balanced in nutrition, can meet Macrobrachium nipponensis nutritional need, improves cultured product quality, improves Macrobrachium nipponensis anti-adversity ability, the mixed feed of water quality deterioration in minimizing breeding process.
The present invention for achieving the above object, adopts following technical scheme:
Macrobrachium nipponensis is anti-stress a mixed feed, comprises by mass percentage following component: crude protein 36.0%-40.0%, crude fat 6%-8%, ash content 7%-9%, carbohydrate 15%-25%, anti-stress additive 1%-2%, moisture 7%-9%.
As preferably, described crude protein is more than one compositions of mixtures in cotton dregs, the dish dregs of rice, fish meal, dregs of beans, peanut meal protein raw materials mainly.
As preferably, described crude fat is mainly fish oil and the soya-bean oil of 1:1.
As preferably, described ash content is mainly calcium dihydrogen phosphate, composite mineral matter and B B-complex.
As preferably, B B-complex consist of (mg g
1): vitamin A, 1.0; Vitamin C, 5; Vitamin E, 10; Vitamin D
3, 0.63; Vitamin K
3, 1.8; Amino benzoic Acid, 5; Thiamine, 0.75; Riboflavin, 2.63; Pyridoxol, 1; Cobalamin, 2; Nicotinic acid, 5; Folic acid, 0.19; Inositol, 60; Biotin, 0.75; Calcium pantothenate, 5; Carrier fibre element, 899.25.
As preferably, composite mineral matter consist of (mg g
1): KCl, 28; MgSO
47H
2o, 100; NaH
2pO
4, 215; KH
2pO
4, 100; Ca (H
2pO
4)
2h
2o, 265; CaCO
3, 105; C
6h
10caO
65H
2o, 165; FeC
6h
5o75H
2o, 12; ZnSO
47H
2o, 4.52; MnSO
4h
2o, 1.07; AlCl
36H
2o, 0.39; CuCl
22H
2o, 0.24; CoCl
26H2O, 1.4; KI, 0.23; Carrier fibre element, 2.15.
As preferably, described carbohydrate is mainly time powder.
As preferably, described anti-stress additive be mainly astaxanthin, glutamine, betaine and the allicin of mass ratio 0.5:1:1:0.5.
Beneficial effect of the present invention:
(1) formula material is selected rationally, and each nutrient is balanced, can meet Macrobrachium nipponensis nutritional need comprehensively, improves Macrobrachium nipponensis growth performance and anti-hypoxemia ability, reduces antibiotic excessive use in water body;
(2) adopt and add the problem that multiple protein raw material has overcome single protein sources amino acid imbalance, promote absorbing of freshwater shrimp, reduce ammonia nitrogen discharge, reduce environmental pollution; The present invention adopts fish oil and soya-bean oil as fat source, and the price of the fish oil of consideration is higher, has saved to a certain extent cost; In the high temperature time, the risk that also part has avoided fish oil easily to become sour;
(3) the present invention from the mechanism of shrimp crab immunity out, anti-ly stress both supplement Chinese herbal medicine and astaxanthin by additive, supplemented again Western medicine glutamine dipeptide; Use after the compatibility agents such as allicin, glutamine, astaxanthin, can overcome the weak point of above-mentioned a certain product, from Chinese and Western bound drug, improve Macrobrachium nipponensis anti-stress ability;
(4) blank greatly of one in current feed formula technology has been filled up in the anti-successful development that stress mixed feed of Macrobrachium nipponensis, solve the series of problems that the immunity function causing due to water body environment is had relatively high expectations declines, cultivation survival rate is lower, do not meet environmental ecology interests in Macrobrachium nipponensis cultivation, met the requirement of Macrobrachium nipponensis large-scale farming, industrialized development.
The specific embodiment
Below in conjunction with specific embodiment, be further described.
Embodiment 1
With 1 formula feed of filling a prescription shown in table 1 (crude protein 38%, crude fat 8%, carbohydrate 15%, ash content 8%, moisture 9%), raise the Macrobrachium nipponensis 8 weeks of about 0.8g.After experiment finishes, the about 3.5g of this group Macrobrachium nipponensis counterpoise, rate of body weight gain 337.5%, protein efficiency ratio, PER 2.05, feed coefficient 1.64, parameters is all compared and is significantly improved with commercial feed group (crude protein 35%, crude fibre 10%, ash content 14%, moisture 12%).Freshwater shrimp Hypoxia Stress (dissolved oxygen is 3mg/L) 7d, the control group death rate be 55.0%, 1.0% anti-stress the additive fish death rate be 36.0%; Compare with control group, 1.0% resists and stress obviously reduce liver concentration of malondialdehyde 6.55% by additive, has increased hemolymph phenoloxidase activity 10.87% and superoxide dismutase activity 8.12%.Therefore, this resists stress can improve the oxidation resistance of Macrobrachium nipponensis by additive, improves Macrobrachium nipponensis hypoxia-resistant capacity, promotes fish bulk-growth.
Embodiment 2
With 2 formula feeds of filling a prescription shown in table 1 (crude protein 40%, crude fat 7%, carbohydrate 20%, ash content 8.5%, moisture 8.8%), raise the Macrobrachium nipponensis 8 weeks of about 1g.After experiment finishes, the about 4.6g of this group Macrobrachium nipponensis counterpoise, rate of body weight gain 388.45%, protein efficiency ratio, PER 2.20, feed coefficient 1.45, parameters is all compared and is significantly improved with commercial feed group (crude protein 35%, crude fibre 10%, ash content 14%, moisture 12%).Freshwater shrimp Hypoxia Stress (dissolved oxygen is 3mg/L) 7d, the control group death rate be 58.0%, 2.0% anti-stress the additive fish death rate be 27.0%; Compare with control group, 2.0% resists and stress obviously reduce liver concentration of malondialdehyde 10.15% by additive, has increased hemolymph phenoloxidase activity 15.65% and superoxide dismutase activity 11.24%.Therefore, resist and stress can improve the oxidation resistance of Macrobrachium nipponensis by additive, improve Macrobrachium nipponensis hypoxia-resistant capacity, promote fish bulk-growth.
Embodiment 3
With 3 formula feeds of filling a prescription shown in table 1 (crude protein 36%, crude fat 6%, carbohydrate 25%, ash content 9%, moisture 7.4%), raise the Macrobrachium nipponensis 8 weeks of about 1g.After experiment finishes, the about 3.8g of this group Macrobrachium nipponensis counterpoise, rate of body weight gain 305.65%, protein efficiency ratio, PER 1.88, feed coefficient 1.78, parameters is all compared and is significantly improved with commercial feed group (crude protein 35%, crude fibre 10%, ash content 14%, moisture 12%).Freshwater shrimp Hypoxia Stress (dissolved oxygen is 3mg/L) 7d, the control group death rate be 54.0%, 3.0% anti-stress the additive fish death rate be 22.0%; Compare with control group, 3.0% resists and stress obviously reduce liver concentration of malondialdehyde 14.37% by additive, has increased hemolymph phenoloxidase activity 18.86% and superoxide dismutase activity 15.12%.Therefore, resist and stress can improve the oxidation resistance of Macrobrachium nipponensis by additive, improve Macrobrachium nipponensis hypoxia-resistant capacity, promote fish bulk-growth.
In above-described embodiment 1-3, the computing formula of each parameter is:
1, the first counterpoise in rate of body weight gain (%)=(whole counterpoise-first counterpoise) * 100/
2, feed coefficient=feedstuff feeding amount/(heavy-initial body weight of whole opisthosoma)
3, protein efficiency ratio, PER=(heavy-initial body weight of whole opisthosoma)/protein feeding volume
4, the death rate (%)=dead shrimp quantity/shrimp sum * 100.
Each feed formula of table 1 and Analysis of Nutritive Composition
In above-mentioned feed formula, B B-complex consist of (mg g
1): vitamin A, 1.0; Vitamin C, 5; Vitamin E, 10; Vitamin D
3, 0.63; Vitamin K
3, 1.8; Amino benzoic Acid, 5; Thiamine, 0.75; Riboflavin, 2.63; Pyridoxol, 1; Cobalamin, 2; Nicotinic acid, 5; Folic acid, 0.19; Inositol, 60; Biotin, 0.75; Calcium pantothenate, 5; Carrier fibre element, 899.25.
Composite mineral matter consist of (mg g
1): KCl, 28; MgSO
47H
2o, 100; NaH
2pO
4, 215; KH
2pO
4, 100; Ca (H
2pO
4)
2h
2o, 265; CaCO
3, 105; C
6h
10caO
65H
2o, 165; FeC
6h
5o75H
2o, 12; ZnSO
47H
2o, 4.52; MnSO
4h
2o, 1.07; AlCl
36H
2o, 0.39; CuCl
22H
2o, 0.24; CoCl
26H2O, 1.4; KI, 0.23; Carrier fibre element, 2.15.
The calculating of crude protein and crude fat is calculated by dry, is about to after feed drying, by triumphant formula azotometer and cable type extractor according, calculates.
Glutamine dipeptide, by Zhejiang, An Mu Chemical Co., Ltd. provides; Described astaxanthin, by Hubei, Heng Lvyuan Science and Technology Ltd. provides; Described betaine (content >90%) and allicin (content >90%) are provided by De Gao bio tech ltd, Jinan.
Claims (7)
1. Macrobrachium nipponensis is anti-stress a mixed feed, comprises by mass percentage following component: crude protein 36.0%-40.0%, crude fat 6%-8%, ash content 7%-9%, carbohydrate 15%-25%, anti-stress additive 1%-2%, moisture 7%-9%;
Described resisting stress additive be astaxanthin, glutamine, betaine and allicin, and mass ratio is 0.5:1:1:0.5.
2. Macrobrachium nipponensis as claimed in claim 1 is anti-stress mixed feed, it is characterized in that: described crude protein derives from two or more in cotton dregs, the dish dregs of rice, fish meal, dregs of beans, peanut meal.
3. Macrobrachium nipponensis as claimed in claim 1 is anti-stress mixed feed, it is characterized in that: described crude fat derives from fish oil and the soya-bean oil of 1:1.
4. Macrobrachium nipponensis as claimed in claim 1 is anti-stress mixed feed, it is characterized in that: described ash content derives from calcium dihydrogen phosphate, composite mineral matter and B B-complex.
5. Macrobrachium nipponensis as claimed in claim 4 is anti-stress mixed feed, it is characterized in that: B B-complex consist of (mg g
1): vitamin A, 1.0; Vitamin C, 5; Vitamin E, 10; Vitamin D
3, 0.63; Vitamin K
3, 1.8; Amino benzoic Acid, 5; Thiamine, 0.75; Riboflavin, 2.63; Pyridoxol, 1; Cobalamin, 2; Nicotinic acid, 5; Folic acid, 0.19; Inositol, 60; Biotin, 0.75; Calcium pantothenate, 5; Carrier fibre element, 899.25.
6. Macrobrachium nipponensis as claimed in claim 4 is anti-stress mixed feed, it is characterized in that: composite mineral matter consist of (mg g
1): KCl, 28; MgSO
47H
2o, 100; NaH
2pO
4, 215; KH
2pO
4, 100; Ca (H
2pO
4)
2h
2o, 265; CaCO
3, 105; C
6h
10caO
65H
2o, 165; FeC
6h
5o75H
2o, 12; ZnSO
47H
2o, 4.52; MnSO
4h
2o, 1.07; AlCl
36H
2o, 0.39; CuCl
22H
2o, 0.24; CoCl
26H2O, 1.4; KI, 0.23; Carrier fibre element, 2.15.
7. Macrobrachium nipponensis as claimed in claim 1 is anti-stress mixed feed, it is characterized in that: described carbohydrate derives from time powder.
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CN104171583B (en) * | 2014-07-11 | 2016-08-17 | 句容市福源水产品养殖场 | A kind of Procambarus clarkii feedstuff and preparation method thereof |
CN104256095A (en) * | 2014-09-26 | 2015-01-07 | 广东恒兴饲料实业股份有限公司 | Compound feed additive having function of protecting intestinal health of tilapia mossambica and preparation method of compound feed additive |
CN104905071A (en) * | 2015-07-02 | 2015-09-16 | 四川国凤中科生物科技有限公司 | Feed for improving the of Macrobrachium nipponense and a production method of feed |
CN105394442A (en) * | 2015-11-16 | 2016-03-16 | 袁生咬 | Special compound high-quality feed additive for freshwater shrimps and preparation method of special compound high-quality feed additive |
CN106551187A (en) * | 2016-11-24 | 2017-04-05 | 佛山市南海东方澳龙制药有限公司 | Improve additive, the feed of prawn anti allergic reaction |
CN109874931B (en) * | 2019-04-22 | 2022-05-17 | 威海金牌生物科技股份有限公司 | Compound feed for improving high-temperature resistance of abalones and preparation method thereof |
CN113142423A (en) * | 2021-03-31 | 2021-07-23 | 中国水产科学研究院长江水产研究所 | Procambarus clarkii anti-stress agent |
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