CN104830719A

CN104830719A - Spirulina culture medium

Info

Publication number: CN104830719A
Application number: CN201510207994.9A
Authority: CN
Inventors: 王兆伟; 彭小伟; 宋佳巍; 杜学星; 孙小雪; 江灵芝; 张君纲; 王弢; 张云; 王�琦; 靳立兵; 王闪; 郑伟
Original assignee: Ningbo Fu Tian Bioisystech Co Ltd
Current assignee: Ningbo Fu Tian Bioisystech Co Ltd
Priority date: 2015-04-24
Filing date: 2015-04-24
Publication date: 2015-08-12
Anticipated expiration: 2035-04-24
Also published as: CN104830719B

Abstract

The invention discloses a spirulina culture medium, which is composed of the following components: sodium bicarbonate (15 to 18 g/L), calcium superphosphate (0.5 g/L), potassium chloride (0.5 g/L), sodium chloride (1 g/L), magnesium sulfate (0.2 g/L), iron citrate (15 mg/L), N2EDTA (8 mg/L), VB1 (0.005 to 0.007 mg/L), VB12 (0.04 to 0.06 mg/L), ZnSO4.4H2O (20 to 26 [mu]g/L), MnCl2.4H2O (160 to 185 [mu]g/L), CuSO4.5H2O (8 to 12 [mu]g/L), Na2MoO4.2H2O (6.8 to 7.5 [mu]g/L), and CoCl2.6H2O (10 to 14 [mu]g/L), and also comprises plant growth regulating substances: brassinolide (0.01 mg/L), diethyl aminoethyl hexanoate (1 mg/L), ethephon (1 mg/L), and rare-earth scandium salts (0.5 to 1 mg/L). The spirulina culture medium has the advantages of high survival rate and quick growth speed of spirulina.

Description

A kind of Spirulina culture medium

Technical field

The present invention relates to a kind of algae media, particularly relate to a kind of Spirulina culture medium.

Background technology

Spirulina is a kind of blue photoautotrophy fine algae, belongs to Cyanophyta, Oscillariaceae, Spirullina.The protein content of spirulina is up to more than 60 ~ 70%, for the ideal protein that Food and Argriculture OrganizationFAO (FAD) is recommended, containing the amino acid that eight kinds of needed by human but can not self synthesize, comprise Methionin, Threonine, methionine(Met), Isoleucine, leucine, tryptophane, phenylalanine, α-amino-isovaleric acid, the human intaking amount of its content in spirulina closely FAD Organisation recommendations, can reasonable supplement and fully absorbing.Spirulina is also containing abundant VITAMIN, polysaccharides, lipoid cpd, carotene, chlorophyll, mineral substance and Phycocyanins, C-etc. in addition, and the cell wall structure fiber of natural spiral seaweed is few, the utilization so its nutritive ingredient is very easily absorbed by the body.The development at home of spirulina breeding industry is growing steadily, and what become health products trade gives priority to object.

Summary of the invention

Technical problem to be solved by this invention is to provide one and is conducive to improving growth of spirulina platensis speed, improves the Spirulina culture medium of yield of spirulina.

For solving the problems of the technologies described above, technical scheme of the present invention is: described Spirulina culture medium comprises the component of following mass concentration: sodium bicarbonate 15-18g/L, calcium superphosphate 0.5g/L, Repone K 0.5g/L, sodium-chlor 1g/L, magnesium sulfate 0.2g/L, ironic citrate 15mg/L, N2EDTA8mg/L, VB10.005-0.007mg/L, VB120.04-0.06mg/L, ZnSO44H2O20-26 μ g/L, MnCl24H2O160-185 μ g/L, CuSO45H2O8-12 μ g/L, Na2MoO42H2O6.8-7.5 μ g/L and CoCl26H2O10-14 μ g/L.

As preferred technical scheme, the formula of described Spirulina culture medium comprises the component of following mass concentration: sodium bicarbonate 15-18g/L, calcium superphosphate 0.5g/L, Repone K 0.5g/L, sodium-chlor 1g/L, magnesium sulfate 0.2g/L, ironic citrate 15mg/L, N2EDTA8mg/L, VB10.005-0.007mg/L, VB120.04-0.06mg/L, ZnSO44H2O20-26 μ g/L, MnCl24H2O160-185 μ g/L, CuSO45H2O8-12 μ g/L, Na2MoO42H2O6.8-7.5 μ g/L and CoCl26H2O10-14 μ g/L, brassinolide 0.01mg/L, amine DA-6 1mg/L, ethrel 1mg/L and rare-earth salts 0.5-1mg/L.

Wherein, described rare-earth salts is scandium salts.

Advantage of the present invention is: Spirulina culture medium of the present invention has when cultivating spirulina the advantage that surviving rate is high, growth of spirulina platensis speed is fast.

Embodiment

Below in conjunction with specific embodiment, Spirulina culture medium provided by the invention is described in detail.

Embodiment:

(1) by the Spirulina culture medium of the following formula of fresh water configuration: sodium bicarbonate 12g/L, calcium superphosphate 0.5g/L, Repone K 0.5g/L, sodium-chlor 1g/L, magnesium sulfate 0.2g/L, ironic citrate 15mg/L, N2EDTA 10mg/L, brassinolide 0.01mg/L, amine DA-6 1mg/L, ethrel 1mg/L, ZnSO44H2O 23 μ g/L, MnCl24H2O178 μ g/L, CuSO45H2O 10 μ g/L, Na2MoO42H2O 7.3 μ g/L, CoCl26H2O 12 μ g/L and scandium salts 0.5mg/L.

(2) spirulina plalensis of taking the logarithm vegetative period is inoculated in this substratum and cultivates, illumination every day 10 hours, intensity of illumination is about 50 μm of ol/ (m2s), and training case temperature controls at 25 degrees Celsius, pass into the air of CO2 containing 0.1%, air flow is 0.2vvm.Record the dry weight (DW) of spirulina in substratum at set intervals.

Comparative example

Use Zarrouk substratum substratum in contrast, the spirulina plalensis of taking the logarithm vegetative period is inoculated in this substratum and cultivates, illumination every day 10 hours, intensity of illumination is about 50 μm of ol/ (m2s), training case temperature controls at 25 degrees Celsius, pass into the air of CO2 containing 0.1%, air flow is 0.2vvm.Record the dry weight (DW) of spirulina in substratum, algae liquid pH value and algae liquid color at set intervals, contrast with using the embodiment of substratum of the present invention, the result of contrast is as shown in table 1 below:

Table 1: the spirulina situation contrast table that different culture media is cultivated

As can be seen from Table 1, the substratum using the embodiment of the present invention to provide can make spirulina breed than using comparative example's substratum (Zarrouk substratum) more quickly, cultivating after 8 days spirulina dry weight (DW) in the embodiment of the present invention is 3.03g/L, in comparative example, spirulina dry weight (DW) is 1.56g/L, biomass improves nearly 90%, illustrates that the substratum of confession of the present invention has obvious cultivation advantage;

The foregoing is only the schematic embodiment of the present invention, and be not used to limit scope of the present invention.Any those skilled in the art, equivalent variations done under the prerequisite not departing from design of the present invention and principle and amendment, all should belong to the scope of protection of the invention.

Claims

1. a Spirulina culture medium, is characterized in that: described Spirulina culture medium comprises the component of following mass concentration: sodium bicarbonate 15-18g/L, calcium superphosphate 0.5g/L, Repone K 0.5g/L, sodium-chlor 1g/L, magnesium sulfate 0.2g/L, ironic citrate 15mg/L, N2EDTA8mg/L, VB10.005-0.007mg/L, VB120.04-0.06mg/L, ZnSO44H2O20-26 μ g/L, MnC124H2O160-185 μ g/L, CuSO45H2O8-12 μ g/L, Na2MoO42H2O6.8-7.5 μ g/L and CoC126H2O10-14 μ g/L.

2. a kind of Spirulina culture medium according to claim 1, it is characterized in that described Spirulina culture medium also has plant growth regulating substance, plant growth regulating substance comprises the component of following mass concentration.Brassinolide 0.01mg/L, amine DA-6 1mg/L, ethrel 1mg/L and rare-earth salts 0.5-1mg/L

3. a kind of Spirulina culture medium according to claim 2, is characterized in that described rare-earth salts is scandium salts.