CN104830719B - A kind of Spirulina culture medium - Google Patents
A kind of Spirulina culture medium Download PDFInfo
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- CN104830719B CN104830719B CN201510207994.9A CN201510207994A CN104830719B CN 104830719 B CN104830719 B CN 104830719B CN 201510207994 A CN201510207994 A CN 201510207994A CN 104830719 B CN104830719 B CN 104830719B
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- spirulina
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a kind of Spirulina culture medium, the Spirulina culture medium includes the component of following mass concentration：The 18g/L of sodium acid carbonate 15, calcium superphosphate 0.5g/L, potassium chloride 0.5g/L, sodium chloride 1g/L, magnesium sulfate 0.2g/L, ironic citrate 15mg/L, N2EDTA 8mg/L, VB10.005 0.007mg/L, VB120.04 0.06mg/L, the μ g/L of ZnSO44H2O20 26, MnCl24H2O160 185 μ g/L, CuSO45H2O8 12 μ g/L, the μ g/L of Na2MoO42H2O6.8 7.5 μ g/L and CoCl26H2O10 14；Also plant growth regulating substance：Brassin lactones 0.01mg/L, diethyl aminoethyl hexanoate 1mg/L, ethephon (CEPHA),2-(chloroethyl) phosphonic acid 1mg/L and the 1mg/L of rare-earth salts scandium salts 0.5.The present invention has the advantages of survival rate is high, growth of spirulina platensis speed is fast when cultivating spirulina.
Description
Technical field
The present invention relates to a kind of algae media, more particularly to a kind of Spirulina culture medium.
Background technology
Spirulina is a kind of blue photoautotrophy fine algae, belongs to Cyanophyta, Oscillariaceae, Spirullina.The egg of spirulina
White matter content is up to more than 60~70%, is FAO (Food and Agriculture Organization of the United Nation)（FAD）The ideal protein of recommendation, must containing eight kinds of human bodies
Need the amino acid that itself can not but synthesize, including lysine, threonine, methionine, isoleucine, leucine, tryptophan, benzene
Alanine, valine, the human intaking amount of the very close FAD Organisation recommendations of its content in spirulina, can rationally be mended
Fill and fully absorb utilization.Other spirulina is also containing abundant vitamin, polysaccharides, lipids, carrotene, Ye Lv
Element, mineral matter and phycocyanin etc., the cell wall structure fiber of natural spiral seaweed is few, so its nutritional ingredient is easily by human body
Absorb.The development of spirulina breeding industry at home is growing steadily, and turn into health products trade gives priority to object.
The content of the invention
The technical problems to be solved by the invention are to provide one kind and are advantageous to improve growth of spirulina platensis speed, improve spirulina
The Spirulina culture medium of yield.
In order to solve the above technical problems, the technical scheme is that：It is dense that the Spirulina culture medium includes following quality
The component of degree：Sodium acid carbonate 15-18g/L, calcium superphosphate 0.5g/L, potassium chloride 0.5g/L, sodium chloride 1g/L, magnesium sulfate 0.2g/
L, ironic citrate 15mg/L, Na2EDTA 8mg/L、VB1 0.005-0.007mg/L、VB12 0.04-0.06mg/L、ZnSO4·
4H2O 20-26μg/L、MnCl2·4H2O 160-185μg/L、CuSO4·5H2O 8-12μg/L、Na2MoO4·2H2O 6.8-
7.5 μ g/L and CoCl2·6H2O 10-14μg/L。
As preferable technical scheme, the formula of the Spirulina culture medium includes the component of following mass concentration：Carbonic acid
Hydrogen sodium 15-18g/L, calcium superphosphate 0.5g/L, potassium chloride 0.5g/L, sodium chloride 1g/L, magnesium sulfate 0.2g/L, ironic citrate
15mg/L、Na2EDTA 8mg/L、VB1 0.005-0.007mg/L、VB12 0.04-0.06mg/L、ZnSO4·4H2O 20-26μ
g/L、MnCl2·4H2O 160-185μg/L、CuSO4·5H2O 8-12μg/L、Na2MoO4·2H2O 6.8-7.5 μ g/L and
CoCl2·6H2O 10-14 μ g/L, brassin lactones 0.01mg/L, diethyl aminoethyl hexanoate 1mg/L, ethephon (CEPHA),2-(chloroethyl) phosphonic acid 1mg/L and rare-earth salts 0.5-
1mg/L。
Wherein, the rare-earth salts is scandium salts.
It is an advantage of the invention that：Spirulina culture medium of the present invention has survival rate height, spiral when cultivating spirulina
The advantages of algae growth rate is fast.
Embodiment
With reference to specific embodiment, Spirulina culture medium provided by the invention is described in detail.Embodiment：
（1）The Spirulina culture medium matched as follows with fresh water configuration：Sodium acid carbonate 15g/L, calcium superphosphate 0.5g/L, chlorine
Change potassium 0.5g/L, sodium chloride 1g/L, magnesium sulfate 0.2g/L, ironic citrate 15mg/L, Na2EDTA 8mg/L,
VB10.005mg/L, VB120.04mg/L brassin lactones 0.01mg/L, diethyl aminoethyl hexanoate 1mg/L, ethephon (CEPHA),2-(chloroethyl) phosphonic acid 1mg/L, ZnSO4
4H2O 23μg/L、MnCl2•4H2O 178μg/L、CuSO4•5H2O 10μg/L、Na2MoO4•2H2O 7.3μg/L、CoCl2•
The μ g/L of 6H2O 12 and scandium salts 0.5mg/L.
（2）The blunt top spirulina in growth period of taking the logarithm is inoculated into the culture medium and cultivated, daily illumination 10 hours,
Intensity of illumination is about 50 μm of ol/ (m2S), case temperature control is trained at 25 degrees Celsius, is passed through containing 0.1% CO2Air, ventilation
Measure as 0.2vvm.The dry weight of spirulina in culture medium is recorded at regular intervals（DW）.
Comparative example
Using Zarrouk culture mediums as control medium, the blunt top spirulina in growth period of taking the logarithm is inoculated into the culture
Cultivated in base, daily illumination 10 hours, intensity of illumination is about 50 μm of ol/ (m2S), it is Celsius 25 to train case temperature control
Degree, is passed through containing 0.1% CO2Air, throughput 0.2vvm.The dry weight of spirulina in culture medium is recorded at regular intervals
（DW）, algae solution pH value and algae solution color, with being contrasted using the embodiment of culture medium of the present invention, the result such as table 1 below of contrast
It is shown：
Table 1：The spirulina situation contrast table of different culture media culture
As it can be seen from table 1 the culture medium ratio provided using the embodiment of the present invention uses comparative example culture medium
（Zarrouk culture mediums）Spirulina can be bred more quickly, spirulina dry weight in the embodiment of the present invention after cultivating 8 days（DW）
For 3.03g/L, spirulina dry weight in comparative example（DW）For 1.56g/L, biomass improves nearly 90%, illustrates present invention confession
Culture medium there is obvious culture advantage；
The schematical embodiment of the present invention is the foregoing is only, is not limited to the scope of the present invention.It is any
Those skilled in the art, made equivalent variations and modification on the premise of the design of the present invention and principle is not departed from,
The scope of protection of the invention should be belonged to.
Claims (1)
- A kind of 1. Spirulina culture medium, it is characterised in that：The Spirulina culture medium includes the component of following mass concentration：Carbonic acid Hydrogen sodium 15g/L, calcium superphosphate 0.5g/L, potassium chloride 0.5g/L, sodium chloride 1g/L, magnesium sulfate 0.2g/L, ironic citrate 15mg/L、Na2EDTA 8mg/L、VB10.005mg/L、VB120.04mg/L brassin lactones 0.01mg/L, diethyl aminoethyl hexanoate 1mg/L, Ethephon (CEPHA),2-(chloroethyl) phosphonic acid 1mg/L, ZnSO4·4H2O 23μg/L、MnCl2·4H2O 178μg/L、CuSO4·5H2O 10μg/L、Na2MoO4· 2H2O 7.3μg/L、CoCl2·6H2The μ g/L of O 12 and scandium salts 0.5mg/L.
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| CN105110942A (en) * | 2015-08-29 | 2015-12-02 | 固镇县传红家庭农场 | Luffa seedling nutrient substrate |
| CN107475141A (en) * | 2016-06-07 | 2017-12-15 | 洛阳华清天木生物科技有限公司 | A kind of Spirulina culture medium |
| CN106509479A (en) * | 2016-09-30 | 2017-03-22 | 防城港市金沙海洋科技有限责任公司 | Special algae culture fertilizer used for aquaculture |
| CN110214653B (en) * | 2019-07-01 | 2021-06-25 | 中国科学院南京土壤研究所 | Method for reducing phosphorus loss of rice field by using periphyton |
| US20210251229A1 (en) * | 2020-02-14 | 2021-08-19 | Suntton Company Limited | Plant regulator compositions |
| CN111172096B (en) * | 2020-04-11 | 2022-04-12 | 杭州渔森农业技术开发有限公司 | Production process for high-density culture of heteroglena |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102080119A (en) * | 2009-11-26 | 2011-06-01 | 北京化工大学 | Method for producing oil by mixed culture of yeast and alga |
| CN102533558A (en) * | 2010-12-22 | 2012-07-04 | 丽江程海保尔生物开发有限公司 | Method for recycling spiral seaweed culture medium |
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| JP5164057B2 (en) * | 2007-07-02 | 2013-03-13 | 国立大学法人 宮崎大学 | Culture medium for photosynthetic organisms using incinerated ash, method for producing the same, and method for culturing photosynthetic organisms |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102080119A (en) * | 2009-11-26 | 2011-06-01 | 北京化工大学 | Method for producing oil by mixed culture of yeast and alga |
| CN102533558A (en) * | 2010-12-22 | 2012-07-04 | 丽江程海保尔生物开发有限公司 | Method for recycling spiral seaweed culture medium |
Non-Patent Citations (1)
| Title |
|---|
| 螺旋藻混合营养培养基配方的优化研究;田华等;《食品科技》;20081231;第33卷(第12期);第32-34页 * |
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