CN103283661A - Nucleated pearl lamella conditioning fluid and application thereof - Google Patents
Nucleated pearl lamella conditioning fluid and application thereof Download PDFInfo
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- CN103283661A CN103283661A CN2013102613072A CN201310261307A CN103283661A CN 103283661 A CN103283661 A CN 103283661A CN 2013102613072 A CN2013102613072 A CN 2013102613072A CN 201310261307 A CN201310261307 A CN 201310261307A CN 103283661 A CN103283661 A CN 103283661A
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- pearl
- lamella
- nucleated
- small pieces
- conditioning fluid
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to nucleated pearl lamella conditioning fluid in the technical field of pearl culture and application of the nucleated pearl lamella conditioning fluid. The conditioning fluid comprises the following constituents, by mass-to-volume concentration percentage, 1-5% of acid red, 0.01-5% of chondroitin, 0.1-5% of mafenide, 0.1-5% of taurine, 0.75% of sodium chloride, 0.1-5% of hyriopsis cumingii polysaccharide, and 0.05-3% of vitronectin, and allowance being water. The invention further relates to the application of the nucleated pearl lamella conditioning fluid in the pearl culture. The nucleated pearl lamella conditioning fluid can strengthen cell viability, prevent cell infection, and accelerate cell division and wound healing. When the nucleated pearl lamella conditioning fluid is applied to culture pearls, the death rate of the pearls is obviously reduced, and the rate of remaining nucleuses is obviously improved. Meanwhile, lamellas can be rapidly attached to lamella insertion shells, and the lamella insertion shells can rapidly proliferate to grow into pearl sacs. The nucleated pearl lamella conditioning fluid is simple in method, easy to achieve, wide in application range and remarkable in effect, is used for cultivating nucleated pearls by fresh water pearl mussels, and has wide application prospects.
Description
Technical field
The invention belongs to the pearl cultivation technology field, be specifically related to a kind of nucleated pearl small pieces treatment fluid and uses thereof.
Background technology
(Hubei Aquatic Scienctific Research Inst.) introduces people when exploring the formation mechanism of pearl in the 4th phase in 2004 " landlocked aquatic products " published article " fresh water genuine pearl with pearl transplanting method ", the core of finding pearl is foreign matters such as gravel, and saying of " foreign matter stimulating into pearl " therefore just arranged.About 13rd century, it is found that the outer embrane secretion pearl of shellfish, therefore, people utilize the mechanism of shellfish jacket membrane secretion pearl and adopt operation method natural imitation foreign matter to stimulate and cultivate nucleated pearl.The operation method that the mechanism of utilizing shellfish jacket membrane to secrete pearl now stimulates in conjunction with foreign matter has become the conventional production methods of nucleated pearl industry.
At present, the breeding method of pearl divides two kinds, and a kind of is to cultivate no nucleus pearl, and another kind is to cultivate nucleated pearl.The major defect that cultivation fresh water does not have nucleus pearl is that culture-cycle large standard high quality pearl ratio long, output is low, cultivates the specification that nucleated pearl can shorten the culture-cycle greatly, improve the pearl of cultivating.But there is more problem in the nucleated pearl breeding method at present.
Flourish along with fresh water pearl culturing, the fresh water pearl culturing technology is ripe gradually.No matter be that fresh water does not have nucleus pearl or nucleated pearl, all need to insert small pieces, make its propagation, grow into pearl sac then and could secrete nacre, form pearl.Thereby the binding ability of small pieces and inserted sheet shellfish, the multiplication capacities of small pieces cell etc. all have a significant impact formation and the growth of pearl.Yet domestic disclosed patent or technology all adopt anti-inflammation drugs, attach promoter, also have combination such as some nutrients simply to handle, and cause row's nuclear rate of pearl culturing clam higher, and small pieces attaching power is not enough, the small pieces poor growth.
For improving quality and the output of pearl, Japan has early carried out the art pre-treatment, the Study on Technology of growing cultured pearls such as small pieces processing, domestic numerous scientific research personnel have also carried out useful exploration to the correlative study of the technology of growing cultured pearls, in the convenient adjustment of carrying out pteria martensii operation shellfish physiological status emphatically of pearly shellfish art pretreatment technology, mainly suppress or promote the gonad growth of shellfish by control discharge, bait etc.; Postoperative mastery mode has also been carried out a large amount of general knowledge; Under cell aspect the sheet processing, open source literature has also been reported the technology of some this respects, main around the vigor that improves cell and secretion function, utilize all kinds of solution-treated small pieces to study, generally soak pearl nuclear with anti-infective or with infrared with the soup of antibiotic configuration, the activating treatment method of ultraviolet irradiation, for example: Chinese patent, application number is: 200810148947.1, the method that a kind of pearl nuclear pharmaceuticals is handled is disclosed, its technical scheme that adopts is: select highly purified gelatin for use, quadracycline, polyvinylpyrrolidone PVP and vitamin C, each component by quality ratio; Add degerming seawater to 1000 milliliter, put 50 ℃ of stirred in water bath, the dissolving of spending the night; In 50～60 ℃ of insulating boxs pearl nuclear is heated to 50～60 ℃, takes out pearl nuclear, load onto described solution with air painter, spraying pearl nuclear surface is constantly stirred, so that the spraying medicine layer is even, smooth and dry simultaneously; Vacuum packaging is transported to the pearl farm, and the conventional nuclear technique of inserting is inserted pearly shellfish, carries out the postoperative recuperation and hangs foster.
More present all kinds of solution-treated small pieces of document utilizations, soup with the antibiotic configuration soaks pearl nuclear, and then anti-infective, though can effectively promote the division of mantle piece cell, accelerate the formation of pearl sac, suppress the operation cell infection, the pearly shellfish survival rate, core-remaining rate, excellent pearl rate has had raising to a certain degree, and in inserting the nuclear surgical procedure, how to promote the epithelial cell division, strengthen cell small pieces vigor and secretion function, promote key technologies such as wound healing still to have shortcoming.
Summary of the invention
At defective of the prior art, the purpose of this invention is to provide a kind of nucleated pearl small pieces treatment fluid and uses thereof.
The present invention is achieved by the following technical solutions,
First aspect the invention provides a kind of nucleated pearl small pieces treatment fluid, and described treatment fluid comprises each component of following quality concentration expressed in percentage by volume:
Azogeramine～5%;
Chondroitin 0.01～5%;
Sulfamylon 0.1～5%;
Taurine 0.1～5%;
Sodium chloride 0.75%;
Swan-mussel polysaccharide 0.1～5%;
Vitronectin 0.05～3%;
Surplus is water.
Preferably, described treatment fluid comprises each component of following quality concentration expressed in percentage by volume:
Acid red 4%;
Chondroitin 0.1%;
Sulfamylon 0.3%;
Taurine 0.75%;
Sodium chloride 0.75%;
Swan-mussel polysaccharide 0.5%;
Vitronectin 0.1%;
Surplus is water.
Second aspect the invention still further relates to the purposes of aforesaid nucleated pearl small pieces treatment fluid in pearl culture.
Preferably, described application comprises the steps: in fresh water pearl culturing clam inserted sheet step, will insert freshwater mussel and soak in described nucleated pearl small pieces treatment fluid with small pieces; Implant afterwards in the freshwater mussel, be implanted into the position insertion pearl nuclear of small pieces again freshwater mussel.
Preferably, the time of described immersion is 5～20 minutes.There is contradiction in the cell small pieces at external vigor and drug absorption ability, if the time is oversize, then cell viability descends, if the time is too short, then the medicine infiltration is few with absorptive amount, can't realize expection treatment effect of the present invention, so could realize best treatment effect in the framework of the present definition.
Compared with prior art, the present invention has following beneficial effect: treatment fluid of the present invention can strengthen cytoactive, prevents cell infection, promotes cell division, promotes wound healing; When small pieces treatment fluid of the present invention was applied to cultivate pearl, remarkable reduction fell in lethality, core-remaining rate significantly improves, and had beyond thought technique effect; Treatment fluid of the present invention can promote small pieces and inserted sheet shellfish to realize fitting fast, and proliferate is pearl sac rapidly; The inventive method is simple, realizes that easily purposes is wide, and effect is remarkable, is used for the fresh water pearl freshwater mussel and cultivates nucleated pearl, has very wide application prospect.
Embodiment
The present invention is described in detail below in conjunction with specific embodiment.Following examples will help those skilled in the art further to understand the present invention, but not limit the present invention in any form.Should be pointed out that to those skilled in the art, without departing from the inventive concept of the premise, can also make some distortion and improvement.These all belong to protection scope of the present invention.
Embodiment 1～7
Embodiment 1～7 relates separately to and utilizes nucleated pearl small pieces treatment fluid to carry out pearl culture, and component and the relation with contents of the small pieces treatment fluid that relates among each embodiment see also table 1, and surplus is water; Wherein, when the quality concentration expressed in percentage by volume refers to be added to solid in the liquid, when solid volume can't be measured, calculate in mass, solid accounts for the percent concentration of the volume of liquid; Wherein, volume is that volume with the volumetric flask constant volume is as the criterion; In embodiment 1, the preparation process of described treatment fluid is as follows: get azogeramine g respectively; Chondroitin 0.01g; Sulfamylon 0.1g; Taurine 0.1g; Sodium chloride 0.75g; Swan-mussel polysaccharide 0.1g; Vitronectin 0.05g, soluble in water afterwards, again solution is settled to 1L, the quality concentration expressed in percentage by volume that namely gets respective components is as shown in table 1.
Utilizing nucleated pearl small pieces treatment fluid to carry out pearl culture specifically comprises the steps: slotting freshwater mussel is soaked in described nucleated pearl small pieces treatment fluid with small pieces; Implant afterwards in the freshwater mussel, be implanted into the position insertion pearl nuclear of small pieces again freshwater mussel.The time of soaking is preferably 5～20 minutes.
Table 1
| Component | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 | Embodiment 7 |
| Acid red | 1 | 2 | 3 | 4 | 4 | 5 | 3 |
| Chondroitin | 0.01 | 0.05 | 0.1 | 0.1 | 0.5 | 2 | 5 |
| Sulfamylon | 0.1 | 0.2 | 0.5 | 0.3 | 1 | 2 | 5 |
| Taurine | 0.1 | 0.5 | 1 | 0.75 | 2 | 4 | 5 |
| Sodium chloride | 0.75 | 0.75 | 0.75 | 0.75 | 0.75 | 0.75 | 0.75 |
| Swan-mussel polysaccharide | 0.1 | 0.5 | 1 | 0.5 | 2 | 3 | 5 |
| Vitronectin | 0.05 | 0.2 | 1 | 0.1 | 2 | 3 | 3 |
Implementation result
For contrasting implementation result of the present invention, will insert freshwater mussel and in physiological saline, carry out immersion treatment with small pieces; Implant afterwards in the freshwater mussel, be implanted into the position insertion pearl nuclear of small pieces again freshwater mussel.The time of soaking also is 5～20 minutes.Result with embodiment 1～7 compares afterwards; For making things convenient for statistics, only insert a pearl nuclear and small pieces in every freshwater mussel; Will small pieces and pearl nuclear put into recuperation pond recuperation 30 days after inserting pearl culturing clam, dead quantity of its interocclusal record inserted sheet shellfish and take off nuclear shellfish quantity, recuperation moves on to plant with freshwater mussel and carries out conventional cultivation after finishing.
The result is as shown in table 2, shows according to the result of table 2: the lethality of freshwater mussel is up to 9.6% in the Comparative Examples, and the present invention handles the lethality of back freshwater mussel and can be reduced to 2.1%; After adopting treatment fluid of the present invention to handle, the freshwater mussel average mortality is 3% only, far below Comparative Examples, and the effect highly significant; The core-remaining rate of Comparative Examples is 63%, and the present invention then can realize the core-remaining rate up to 91%; After adopting treatment fluid of the present invention to handle, average core-remaining rate is 84.8%, has improved 34.6% than Comparative Examples, has produced beyond thought technique effect.Simultaneously as can be seen, the corresponding lethality of the treatment fluid that embodiment 4 relates to and core-remaining rate all have best effect.
Table 2
In the treatment fluid of the present invention, the effect of sulfamylon is antibacterial rather than sterilization, thereby its concentration will remain within the framework of the present definition, and it absorbs back degraded along with medicine in certain hour (4～6 hours) back, its antibacterial ability decline through cell; Because small pieces are that time of pearl sac is longer in hyperplasia, the wound healing time of pearl culturing clam is also longer, therefore needs the produced simultaneously immunity of taurine could make pearl culturing clam avoid wound infection, and taurine can improve the body non-specific immune function; Swan-mussel polysaccharide can be bred the enhancing body's immunity that is converted by promoting immunocyte (mainly being lymphocyte); Within the particular range that the present invention limits, sulfamylon, taurine, three kinds of components of swan-mussel polysaccharide can be brought into play significant synergy, promote the wound healing of pearl culturing clam, anti-inflammation, realize that lethality reduces about 80%, significantly reduce the lethality of pearl culturing clam, produced beyond thought technique effect;
Treatment fluid of the present invention waits the demand of oozing for satisfying, and the content of sodium chloride is specific to be 0.75%; Simultaneously, chondroitin has water conservation and lubricant effect, can adion, promote the glass mucoprotein to attaching and the multiplication capacity of small pieces cell, promote attaching and the propagation of small pieces and pearl nuclear, produce synergy, significantly improve core-remaining rate, make that average core-remaining rate is 84.8%, improved 34.6% than prior art, have beyond thought technique effect.The organic cooperation of each component for the treatment of fluid of the present invention can produce significant synergy in specific scope and so on, and formation effect system has beyond thought technique effect;
In sum, treatment fluid of the present invention can strengthen cytoactive, prevents cell infection, promotes cell division, promotes wound healing; When small pieces treatment fluid of the present invention was applied to cultivate pearl, remarkable reduction fell in its lethality, core-remaining rate significantly improves, and had beyond thought technique effect; Treatment fluid of the present invention can promote small pieces and inserted sheet shellfish to realize fitting fast, and proliferate is pearl sac rapidly; The inventive method is simple, realizes that easily purposes is wide, and effect is remarkable, is used for the fresh water pearl freshwater mussel and cultivates nucleated pearl, has very wide application prospect.
The above, it only is preferred embodiment of the present invention, be not that the present invention is done any pro forma restriction, any content that does not break away from technical solution of the present invention, to any simple modification, equivalent variations and modification that above embodiment does, all belong to the scope of technical solution of the present invention according to technical spirit of the present invention.
Claims (5)
1. a nucleated pearl small pieces treatment fluid is characterized in that, described treatment fluid comprises each component of following quality concentration expressed in percentage by volume:
Azogeramine～5%;
Chondroitin 0.01～5%;
Sulfamylon 0.1～5%;
Taurine 0.1～5%;
Sodium chloride 0.75%;
Swan-mussel polysaccharide 0.1～5%;
Vitronectin 0.05～3%;
Surplus is water.
2. nucleated pearl small pieces treatment fluid as claimed in claim 1 is characterized in that, described treatment fluid comprises each component of following quality concentration expressed in percentage by volume:
Acid red 4%;
Chondroitin 0.1%;
Sulfamylon 0.3%;
Taurine 0.75%;
Sodium chloride 0.75%;
Swan-mussel polysaccharide 0.5%;
Vitronectin 0.1%;
Surplus is water.
3. the purposes of a nucleated pearl small pieces treatment fluid as claimed in claim 1 in pearl culture.
4. purposes as claimed in claim 3 is characterized in that, described application comprises the steps: in fresh water pearl culturing clam inserted sheet step, will insert freshwater mussel and soak in described nucleated pearl small pieces treatment fluid with small pieces; Implant afterwards in the freshwater mussel, be implanted into the position insertion pearl nuclear of small pieces again freshwater mussel.
5. purposes as claimed in claim 4 is characterized in that, the time of described immersion is 5～20 minutes.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103947593A (en) * | 2014-05-04 | 2014-07-30 | 千足珍珠集团股份有限公司 | Method for cultivating nucleus pearls |
| CN103947592A (en) * | 2014-05-04 | 2014-07-30 | 千足珍珠集团股份有限公司 | Treating fluid used for cultivation of nucleus pearls |
| CN107593543A (en) * | 2017-10-24 | 2018-01-19 | 浙江海洋大学 | A kind of fresh water genuine pearl with pearl mantle piece treatment fluid and preparation method thereof |
| CN113711960A (en) * | 2021-09-30 | 2021-11-30 | 北部湾大学 | Method for reducing seawater pearl shell heterograft immune rejection |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103947593A (en) * | 2014-05-04 | 2014-07-30 | 千足珍珠集团股份有限公司 | Method for cultivating nucleus pearls |
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| CN103947592B (en) * | 2014-05-04 | 2016-03-02 | 千足珍珠集团股份有限公司 | For the treatment fluid that nucleated pearl is cultivated |
| CN103947593B (en) * | 2014-05-04 | 2016-04-13 | 千足珍珠集团股份有限公司 | The breeding method of nucleated pearl |
| CN107593543A (en) * | 2017-10-24 | 2018-01-19 | 浙江海洋大学 | A kind of fresh water genuine pearl with pearl mantle piece treatment fluid and preparation method thereof |
| CN113711960A (en) * | 2021-09-30 | 2021-11-30 | 北部湾大学 | Method for reducing seawater pearl shell heterograft immune rejection |
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Application publication date: 20130911 |