CN103330717B - Pearl nucleus treatment liquid for freshwater nucleated pearl, application of pearl nucleus treatment liquid and treatment method of freshwater nucleated pearl - Google Patents
Pearl nucleus treatment liquid for freshwater nucleated pearl, application of pearl nucleus treatment liquid and treatment method of freshwater nucleated pearl Download PDFInfo
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- CN103330717B CN103330717B CN201310260411.XA CN201310260411A CN103330717B CN 103330717 B CN103330717 B CN 103330717B CN 201310260411 A CN201310260411 A CN 201310260411A CN 103330717 B CN103330717 B CN 103330717B
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- pearl
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to pearl nucleus treatment liquid for freshwater nucleated pearl, application of the pearl nucleus treatment liquid and a treatment method of freshwater nucleated pearl. The treatment liquid comprises the following components in percentage by mass: 0.05-3 percent of hyaluronic acid, 0.75 percent of sodium chloride, 0.05-5 percent of hyriopsis cumingii polysaccharide, 0.05-5 percent of chondroitin and the balance of water. The invention also relates to the application of the pearl nucleus treatment liquid in pearl culture and the pearl nucleus treatment method of the freshwater nucleated pearl. By adopting the pearl nucleus treated with the treatment liquid, the exclusion of a clam against the pearl nucleus can be reduced, the pearl nucleus retaining rate of nucleus inserted clams is increased, and the nucleus treatment liquid has a very wide application prospect.
Description
Technical field
The invention belongs to pearl cultivation technology field, be specifically related to a kind of fresh water genuine pearl with pearl pearl core treatment fluid, purposes and processing method.
Background technology
Since ancient times, pearl as " queen " in jewelry by people are treasured.Flourish along with pearl culture, pearl production improves constantly, and people are to large and demand that the is pearl of circle increases.Pearl culture family needs to carry out slotting core process to fresh water production pearl, make pearl shaped rounded while in the identical cultivation time limit, pearl diameter is larger.At present, the many shells by large-scale shellfish of pearl core grind and form, because they are different from slotting core freshwater mussel kind, the rejection of freshwater mussel body self causes that to tell core rate higher, and pearl core is after initial rounding polishing, if directly insert freshwater mussel body, slotting core freshwater mussel should be caused dead.Therefore, reduce freshwater mussel body to the rejection of pearl core, improving the core-remaining rate inserting core freshwater mussel is a great problem producing fresh water genuine pearl with pearl.
For improving the Quality and yield of pearl, Japan has comparatively early carried out preoperative medication, the research of the technology of growing cultured pearls such as small pieces process, domestic numerous scientific research personnel have also carried out useful exploration to the correlative study of the technology of growing cultured pearls, in the convenient adjustment having carried out emphatically pteria martensii art shellfish physiological status of pearly shellfish preoperative medication technology, mainly through controlling the gonadal development that discharge, bait etc. suppress or promotes shellfish, a large amount of general knowledge has also been carried out to postoperative mastery mode, in mantle piece process, open source literature also reports the technology of some this respects, mainly around the vigor and the Secretion that improve cell, all kinds of solution-treated small pieces are utilized to study, generally soak pearl core with anti-infective or with infrared with the liquid of antibiotic configuration, the activating treatment method of ultraviolet irradiation, such as: Chinese patent, application number is: 200810148954.1, disclose a kind of method processing mantle piece raising water pearl quality, its technical method is: the chitosan oligosaccharide selecting separation and purification, the solution that concentration is 0.5-3% is mixed with degerming seawater, the peripheral edge portion with the outer embrane non-pigment particle strengthening splitting ability is cut outer embrane from mantle piece shellfish, soak mantle piece after 5-10 minute, or similar concentration solution smears rear placement about 10 minutes, then be cut into long and the wide mantle piece being 4.5-5.5 millimeter, what easily allowed by chitosan oligosaccharide the mantle piece of process implant pinctada together with pearl grows cultured pearls in bag, recuperate after 15 days, and the pearly shellfish of the outer embrane bottom sheet of implantation pearl core and chitosan oligosaccharide process is transferred in marine culture water grows cultured pearls.
All kinds of solution-treated small pieces of some document utilizations at present, pearl core is soaked with the liquid of antibiotic configuration, and then can be anti-infective, although the division of mantle piece cell effectively can be promoted, accelerate the formation of pearl sac, suppress operation cell infection, pearly shellfish survival rate, core-remaining rate, excellent pearl rate has had raising to a certain degree, and how to promote that epithelial cell divides in slotting core surgical procedure, to strengthen mantle piece vigor and the key technology such as Secretion, promotion wound healing and still have shortcoming.
Summary of the invention
For defect of the prior art, the object of this invention is to provide a kind of fresh water genuine pearl with pearl pearl core treatment fluid, purposes and processing method.
The present invention is achieved by the following technical solutions,
First aspect, the present invention relates to a kind of fresh water genuine pearl with pearl pearl core treatment fluid, described treatment fluid comprises the component of following quality concentration expressed in percentage by volume:
Hyaluronic acid 0.01 ~ 3%;
Sodium chloride 0.75%;
Swan-mussel polysaccharide 0.05 ~ 5%;
Chondroitin 0.05 ~ 5%;
Surplus is water.
Preferably, described treatment fluid comprises the component of following quality concentration expressed in percentage by volume:
Hyaluronic acid 0.05%;
Sodium chloride 0.75%;
Swan-mussel polysaccharide 0.2%;
Chondroitin 3%;
Surplus is water.
In pearl core treatment fluid of the present invention, hyaluronic acid has water retention characteristic, in the concentration range that the present invention limits, hyaluronic acid is formed with facilitation to fibroblast and capillary network, the extracellular matrix components such as fibroblasts to secrete precollagen, fibronectin and clostridiopetidase A, can migrate to wound in wound healing process breeds; Chondroitin can inflammation-inhibiting; Swan-mussel polysaccharide is by promoting that the propagation of macrophage improves body immunity; Hyaluronic acid, chondroitin, swan-mussel polysaccharide three, in the concentration range that the present invention limits, can play synergy, the generation of effective inflammation-inhibiting, promote to grow cultured pearls wound healing, significantly improve the compatibility of pearl core and slotting core, reduce and tell core rate, substantially increase core-remaining rate.The inventive method is simple, and easily realize, Be very effective, efficiency is high, for the production of nucleus pearl field, has very wide application prospect.
Second aspect, the present invention relates to the application of aforesaid fresh water genuine pearl with pearl pearl core treatment fluid in pearl culture.
The third aspect, the invention still further relates to a kind of processing method of fresh water genuine pearl with pearl pearl core, comprises the steps: that pearl core being put into aforesaid treatment fluid soaks.
Preferably, the processing method of described fresh water genuine pearl with pearl pearl core comprises the steps: to get pearl core, carries out the process of plating alkoxy silane resin molding; Put into treatment fluid afterwards again to soak.
Preferably, described resin is organic siliconresin.
Preferably, described resin is alkoxy silane.
Preferably, described alkoxy silane is the mixing of one or more in tetramethoxy-silicane, MTMS, MTES, ethyl triethoxy silicane alkane, three second triethoxysilanes, vinyltrimethoxy silane, 3-trimethoxy silane acrylic acid third fat.
Preferably, the process of described plated resin film comprises the steps:, by the method be coated with, brush, spray or soak, to form resin molding, toast afterwards on pearl core surface.
Preferably, described immersion comprises the steps: pearl core to be soaked 15 minutes ~ 2 hours in resin solution, drying at room temperature 1 ~ 3 hour, and 120 DEG C harden 4 ~ 24 hours.
Preferably, the mass percent concentration of described resin solution is 5% ~ 20%.
Preferably, toast 15 ~ 60 minutes at described baking is specially 120 DEG C.
Compared with prior art, the present invention has following beneficial effect: treatment fluid of the present invention can significantly improve the compatibility of pearl core and slotting core, reduces simultaneously and tells core rate, improve core-remaining rate; The inventive method is simple, and easily realize, Be very effective, efficiency is high, for the production of nucleus pearl field, has very wide application prospect.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.Following examples will contribute to those skilled in the art and understand the present invention further, but not limit the present invention in any form.It should be pointed out that to those skilled in the art, without departing from the inventive concept of the premise, some distortion and improvement can also be made.These all belong to protection scope of the present invention.
embodiment 1
Dissolve in methyl alcohol by a certain amount of tetramethoxy-silicane, the mass volume ratio of tetramethoxy-silicane and methyl alcohol is 5%, obtained resin solution.Pearl core is soaked air drying at room temperature 2 hours after 15 minutes in this solution, then 120 DEG C of sclerosis 6 hours.Pearl core after overlay film is put into the following aqueous solution soak: hyaluronic acid 0.01%, sodium chloride 0.75%, swan-mussel polysaccharide 0.1%, chondroitin 0.05%, 70 DEG C of water-baths soak 48 hours.
Wherein, when quality concentration expressed in percentage by volume refers to and solid is added in liquid, when solid volume cannot be measured, calculate in mass, solid accounts for the percent concentration of the volume of liquid; Wherein, volume is as the criterion with the volume of volumetric flask constant volume; In the present embodiment, the preparation process of described treatment fluid is as follows: get hyaluronic acid 0.01g respectively, sodium chloride 0.75g, swan-mussel polysaccharide 0.1g, chondroitin 0.05g, soluble in water afterwards, again solution is settled to 1L, the quality concentration expressed in percentage by volume obtaining respective components is as shown in table 1.
embodiment 2
Dissolve in methyl alcohol by a certain amount of MTMS, the mass volume ratio of MTMS and methyl alcohol is 8%, obtained resin solution.Pearl core is soaked air drying at room temperature 1 hour after 20 minutes in this solution, then 120 DEG C of sclerosis 4 hours.Pearl core after overlay film is put into the following aqueous solution soak: hyaluronic acid 0.1%, sodium chloride 0.75%, swan-mussel polysaccharide 0.05%, chondroitin 0.1%, 70 DEG C of water-baths soak 36 hours.
embodiment 3
Dissolve in ethanol by a certain amount of MTES, the mass volume ratio of MTES and ethanol is 10%, obtained resin solution.Pearl core is soaked air drying at room temperature 1.5 hours after 25 minutes in this solution, then 120 DEG C of sclerosis 9 hours.Pearl core after overlay film is put into the following aqueous solution soak: hyaluronic acid 0.2%, sodium chloride 0.75%, swan-mussel polysaccharide 0.15%, chondroitin 0.3%, 75 DEG C of water-baths soak 48 hours.
embodiment 4
Dissolve in ethanol by a certain amount of alkane of ethyl triethoxy silicane, the mass volume ratio of ethyl triethoxy silicane alkane and ethanol is 12%, obtained resin solution.This dissolution homogeneity is spread upon pearl surface, and then, then 120 DEG C are toasted 15 minutes.Pearl core after overlay film is put into the following aqueous solution soak: hyaluronic acid 0.4%, sodium chloride 0.75%, swan-mussel polysaccharide 0.5%, chondroitin 0.75%, 75 DEG C of water-baths soak 48 hours.
embodiment 5
Dissolve in methyl alcohol by a certain amount of vinyltrimethoxy silane, the mass volume ratio of vinyltrimethoxy silane and methyl alcohol is 12%, obtained resin solution.Pearl core is soaked air drying at room temperature 3 hours after 45 minutes in this solution, then 120 DEG C of sclerosis 20 hours.Pearl core after overlay film is put into the following aqueous solution soak: hyaluronic acid 1%, sodium chloride 0.75%, swan-mussel polysaccharide 1.5%, chondroitin 1%, 80 DEG C of water-baths soak 54 hours.
embodiment 6
Dissolve in methyl alcohol by a certain amount of MTES, the mass volume ratio of MTES and methyl alcohol is 15%, obtained resin solution.Pearl core is soaked air drying at room temperature 2 hours after 30 minutes in this solution, then 120 DEG C of sclerosis 8 hours.Pearl core after overlay film is put into the following aqueous solution soak: hyaluronic acid 0.05%, sodium chloride 0.75%, swan-mussel polysaccharide 0.2%, chondroitin 3%, 80 DEG C of water-baths soak 54 hours
embodiment 7
Dissolve in ethanol by a certain amount of three second triethoxysilanes, the mass volume ratio of three second triethoxysilanes and ethanol is 15%, obtained resin solution.By this solution spraying after pearl surface, then 120 DEG C are toasted 30 minutes.Pearl core after overlay film is put into the following aqueous solution soak: hyaluronic acid 2%, sodium chloride 0.75%, swan-mussel polysaccharide 5%, chondroitin 1.5%, 75 DEG C of water-baths soak 64 hours.
embodiment 8
Enter in isopropyl alcohol by the liposoluble of a certain amount of 3-trimethoxy silane acrylic acid third, the mass volume ratio of 3-trimethoxy silane acrylic acid third fat and isopropyl alcohol is 20%, obtained resin solution.This dissolution homogeneity scrubbing brush is coated in pearl core surface.Then 120 DEG C are toasted 60 minutes.Pearl core after overlay film is put into the following aqueous solution soak: hyaluronic acid 3%, sodium chloride 0.75%, swan-mussel polysaccharide 2%, chondroitin 5%, 70 DEG C of water-baths soak 36 hours.
embodiment 9
The pearl core of select is put into the following aqueous solution soak: hyaluronic acid 1.5%, sodium chloride 0.75%, swan-mussel polysaccharide 0.75%, chondroitin 3%, 75 DEG C of water-baths soak 30 hours.
embodiment 10
The pearl core of select is put into the following aqueous solution soak: hyaluronic acid 2.5%, sodium chloride 0.75%, swan-mussel polysaccharide 1%, chondroitin 2%, 80 DEG C of water-baths soak 45 hours.
implementation result
Process as a comparison, pearl core is immersed in physiological saline and soaks 24h, afterwards small pieces and pearl core are inserted pearl culturing clam.The pearl core simultaneously adopting embodiment 1 ~ 10 to process and small pieces insert pearl culturing clam.Put into recuperation pond recuperation 30 days afterwards, the dead quantity of its interocclusal record inserted sheet shellfish and de-core shellfish quantity, after recuperation terminates, move on to plant and carry out conventional cultivation by freshwater mussel;
Result is as shown in table 2, and in comparative example, core-remaining rate is only 49%, and the embodiment of the present invention is minimum also can reach 68%, reaches as high as 92%, has beyond thought technique effect; Meanwhile, the process of plated resin film is first carried out to pearl core, re-use treatment fluid of the present invention and soak, the rejection of freshwater mussel body to pearl core can be lowered further, improve the core-remaining rate inserting core freshwater mussel; Visible, treatment fluid of the present invention can significantly improve the compatibility of pearl core and slotting core, reduces simultaneously and tells core rate, improve core-remaining rate; The inventive method is simple, and easily realize, Be very effective, efficiency is high, for the production of nucleus pearl field, has very wide application prospect.
The pearl core prescription for the treatment of liquid of table 1 embodiment 1 ~ 10
Table 2 implementation result
Above specific embodiments of the invention are described.It is to be appreciated that the present invention is not limited to above-mentioned particular implementation, those skilled in the art can make various distortion or amendment within the scope of the claims, and this does not affect flesh and blood of the present invention.
Claims (9)
1. a fresh water genuine pearl with pearl pearl core treatment fluid, is characterized in that, described treatment fluid comprises the component of following quality concentration expressed in percentage by volume:
Hyaluronic acid 0.01 ~ 3%;
Sodium chloride 0.75%;
Swan-mussel polysaccharide 0.05 ~ 5%;
Chondroitin 0.05 ~ 5%;
Surplus is water.
2. fresh water genuine pearl with pearl pearl core treatment fluid as claimed in claim 1, it is characterized in that, described treatment fluid comprises the component of following quality concentration expressed in percentage by volume:
Hyaluronic acid 0.05%;
Sodium chloride 0.75%;
Swan-mussel polysaccharide 0.2%;
Chondroitin 3%;
Surplus is water.
3. a processing method for fresh water genuine pearl with pearl pearl core, is characterized in that, comprises the steps: that pearl core being put into treatment fluid according to claim 1 soaks.
4. the processing method of fresh water genuine pearl with pearl pearl core as claimed in claim 3, is characterized in that, comprise the steps: to get pearl core, carry out the process of plating alkoxy silane resin molding; Put into treatment fluid afterwards again to soak.
5. the processing method of fresh water genuine pearl with pearl pearl core as claimed in claim 4, it is characterized in that, described alkoxy silane is the mixing of one or more in tetramethoxy-silicane, MTMS, MTES, ethyl triethoxy silicane alkane, vinyltrimethoxy silane, 3-trimethoxy silane acrylic acid third fat.
6. the processing method of fresh water genuine pearl with pearl pearl core as claimed in claim 4, is characterized in that, the process of described plated resin film comprises the steps:, by the method be coated with, brush, spray or soak, to form resin molding, toast afterwards on pearl core surface.
7. the processing method of fresh water genuine pearl with pearl pearl core as claimed in claim 4, it is characterized in that, described immersion comprises the steps: pearl core to be soaked 15 minutes ~ 2 hours in resin solution, drying at room temperature 1 ~ 3 hour, and 120 DEG C harden 4 ~ 24 hours.
8. the processing method of the fresh water genuine pearl with pearl pearl core stated as claim 7, it is characterized in that, the mass percent concentration of described resin solution is 5% ~ 20%.
9. the processing method of fresh water genuine pearl with pearl pearl core as claimed in claim 6, is characterized in that, toasts 15 ~ 60 minutes at described baking is specially 120 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310260411.XA CN103330717B (en) | 2013-06-26 | 2013-06-26 | Pearl nucleus treatment liquid for freshwater nucleated pearl, application of pearl nucleus treatment liquid and treatment method of freshwater nucleated pearl |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310260411.XA CN103330717B (en) | 2013-06-26 | 2013-06-26 | Pearl nucleus treatment liquid for freshwater nucleated pearl, application of pearl nucleus treatment liquid and treatment method of freshwater nucleated pearl |
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| CN103330717A CN103330717A (en) | 2013-10-02 |
| CN103330717B true CN103330717B (en) | 2015-03-25 |
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| CN105075959B (en) * | 2015-10-05 | 2017-11-03 | 江毓锋 | It is a kind of to cultivate the pearl nucleus of short cycle nucleated pearl |
| CN107593543B (en) * | 2017-10-24 | 2020-03-10 | 浙江海洋大学 | Fresh water nucleated pearl cell pellet treatment fluid and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| AU3406800A (en) * | 2000-05-12 | 2001-11-22 | Koken Co., Ltd. | A coated nucleus for a cultured pearl |
| CN101352154A (en) * | 2008-09-12 | 2009-01-28 | 海南大学 | Method for processing mantle piece and improving quality of sea water pearl |
| CN101356901B (en) * | 2008-09-12 | 2011-01-26 | 海南大学 | Method for pearl core treatment by medicament |
| CN101803580A (en) * | 2010-04-21 | 2010-08-18 | 广西壮族自治区海洋研究所 | Pearl nucleus active liquid used for saltwater pearl culture and preparation method thereof |
| CN102550451B (en) * | 2011-12-27 | 2013-09-18 | 浙江山下湖珍珠集团股份有限公司 | Pearl cytoactive liquid and application of pearl cytoactive liquid to pearl culture |
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Effective date of registration: 20170228 Address after: 311804 Industrial Park, Yamashita Town, Zhejiang, Zhuji Patentee after: Zhejiang foot Jewelry Co., Ltd. Address before: Hu Zhen Zheng Jia Hu Cun 311804 Zhejiang city of Shaoxing province Zhuji City mountain Patentee before: Pure Pearl Group Co., Ltd. |
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