CN102246715A - Breeding method of night fluorescent pearl oyster - Google Patents
Breeding method of night fluorescent pearl oyster Download PDFInfo
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- CN102246715A CN102246715A CN2011101175527A CN201110117552A CN102246715A CN 102246715 A CN102246715 A CN 102246715A CN 2011101175527 A CN2011101175527 A CN 2011101175527A CN 201110117552 A CN201110117552 A CN 201110117552A CN 102246715 A CN102246715 A CN 102246715A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a breeding method of a night fluorescent pearl oyster, comprising the following steps of: uniformly mixing night fluorescent powder with epoxide resin; coating an obtained mixture on the surface of a pearl nucleus; curing, and coating methyl methacrylate as a protecting layer; treating by using a pearl nucleus adjusting agent prepared from algal polysaccharides and aureomycin; treating cell shells by using an adjusting agent prepared from shellfish meat hydrolyzate same as that for a pearl oyster (mussel), and aureomycin, and carrying out an pearl-nucleus-inserting operation; and then fostering a freshwater mussel or pearl-breeding shellfish in a recuperation pool temporarily after the pearl-nucleus-inserting operation, then placing the freshwater mussel or pearl-breeding shellfish in a breeding water area for hoisted breeding, and harvesting after maturity to obtain a pearl, namely a night fluorescent pearl. According to the breeding method of the night fluorescent pearl oyster, the problems that the traditional pearls have single kind and low added value as well as the pearl-nucleus-inserting operation has low nucleus reserving rate and great repelling force are mainly solved; the ornament and the added value of the pearls are increased; and the nucleus splitting rate and the repelling force of the pearl oyster subjected to the operation are reduced.
Description
Technical field
The present invention relates to the pearl cultivation technology field, particularly a kind of night the fluorescence pearl shell breeding method.
Background technology
At present, China pearl presents " high yield low value " phenomenon, this has seriously restricted the development of pearl sector, and 2005 annual productions reach 1500 tons, accounts for global more than 95%, but its output value accounts for the 5%-10% of world's pearl gross output value, wherein the pearl more than 80% can only be sold to ground such as Hong Kong, Japan and America and Europe with the form of raw material or primary processed items, increases the pearl kind so China presses for, and changes former single type, cultivate the pearl of diversity, characteristic, promote its added value.The market pearl is many with artificial culture dyeing or later stage dyeing now, in the hope of increasing the diversity of pearl color, satisfies consumer demand.But the two weak point is that artificial culture generally is with the pearl nuclear staining, and its time length can be faded, and the later stage dyed pearls can only dye light color, and dyeing holds in close confidence, if dye dark color, with the passing of time also can fade.
The self-control luminescent powder is nontoxic, "dead"; Algal polysaccharides not only provides carbohydrate for the shellfish body, can also enhancing body immunity, promote wound healing, effects such as anti-inflammatory; Aureomycin can kill the susceptible bacteria of operation shellfish wound; Shellfish meat hydrolyzate solvent is identical with the shellfish body, thus have bioaffinity, and, tell nuclear rate and repulsive force thereby reduce the shellfish body for the shellfish body provides nutriment.Find that by retrieval by self-control luminescent powder coating pearl nuclear surface, sodium alginate and aureomycin are handled pearl nuclear, shellfish meat hydrolyzate and aureomycin are handled the cell small pieces and be yet there are no report from the technology of growing cultured pearls that row are used.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, provide a kind of night the fluorescence pearl cultivating method, not only increase the pearl added value, also can improve the pearl core-remaining rate, reduce the repulsive force of shellfish body to pearl nuclear.
Technical solution of the present invention is as follows:
The self-control luminescent powder mixes by 10%-15% weight ratio and epoxy resin, is coated in pearl nuclear surface, and after solidifying in 20-24 hour, being coated with methyl methacrylate is overcoat; Pearl nuclear was soaked 5-10 minute in containing the pearl nuclear conditioning agent of algal polysaccharides and aureomycin, the cell small pieces soak after 1-2 minute in the cell small pieces conditioning agent that contains aureomycin and shellfish body hydrolyzate, insert the nuclear operation again; After the operation freshwater mussel or pearly shellfish are supported temporarily in the recuperation pond, after put back to and culture the waters and hang fosterly, obtain fluorescence pearl shell at night, ripe results pearl is fluorescence pearl at night.
The preparation method of above-mentioned luminescent powder is: mol ratio is 4: 1: 4 Al
2O
3, Al (OH)
3And Eu
2O
3Mixture, add 1%-3% weight H
3BO
3As cosolvent, fully mix grinding is even, and 1400 ℃ of sintering, sintering time are 2.5-3 hour; Take out and also to pulverize intermediate product, sintering in 1500 ℃ of nitrogen, taking out and pulverizing the back is the KOH solution washing of 1%-3% with concentration, until pH less than 10.5; Filter the 70-80 ℃ of oven dry in back, porphyrize with the screening of 10000 eye mesh screens, promptly gets luminescent powder.
The preparation method of above-mentioned pearl nuclear conditioning agent is: in the aseptic operating desk of washing one's face with seawater through 0.45 μ m membrane filtration, press the 1%-2% weight ratio and add algal polysaccharides, carry out packing behind the swelling mixing, add aureomycin during nuclear to be inserted and make its concentration reach every milliliter of 1000-2000 SI units, promptly get pearl and examine conditioning agent.
The preparation method of above-mentioned cell small pieces conditioning agent is: with distilled water be feed shellfish (freshwater mussel) meat after 0.6%-0.9% bacillus subtilis neutral proteinase solution, 1.2%-1.7% trypsin solution, 0.4%-0.8% pepsin solution and the homogenate of thawing of solvent by 1: 1: 1: 1 weight ratio is prepared; Shellfish (freshwater mussel) meat thaws and regulates pH to 8.0-8.5 after the homogenate, and 40 ℃-50 ℃ of temperature add bacillus subtilis neutral proteinase solution, hydrolysis 3.5-4 hour; Regulate pH to 6-6.5 afterwards, 35 ℃-40 ℃ of temperature add trypsin solution, hydrolysis 3-4 hour; Regulate pH to 3.0 afterwards, 55 ℃-60 ℃ of temperature add pepsin solution, hydrolysis 3-4 hour again; Add the decolorizer that 1.5%-2% mixes by weight with active carbon and diatomite at 2: 3 then, handle after 50-70 minute for 60-70 ℃ and pack, add aureomycin during nuclear to be inserted and make its concentration reach every milliliter of 1000-2000 SI units, promptly get the small pieces conditioning agent.
Compared with prior art, the present invention has following advantage:
1) the self-control luminescent powder is nontoxic, "dead", and fluorescent lifetime is long;
2) the promising shellfish body of algal polysaccharides provides carbohydrate, and enhancing body immunity promotes wound healing, effects such as anti-inflammatory;
3) aureomycin can kill the susceptible bacteria of operation shellfish wound;
4) shellfish meat hydrolyzate solvent is identical with the shellfish body, thus have bioaffinity, and for the shellfish body provides nutriment, reduce the shellfish body and tell nuclear rate and repulsive force.
Embodiment
Embodiment 1
Cultivation pteria martensii fluorescence at night pearl shell can be divided into following steps to carry out:
1) luminescent powder coating pearl nuclear:
With Al
2O
3, Al (OH)
3, Eu
2O
34: 1: 4 in molar ratio, add 1% (by weight percentage) H again
3BO
3As cosolvent, the abundant mix grinding of above raw material is even, 1400 ℃ of sintering, sintering time is 2.5 hours, takes out and the pulverizing intermediate product, and is synthetic in 1500 ℃ of nitrogen atmospheres, take out to pulverize the back again with 1% KOH solution washing, reach 10.0, sample is filtered through filter paper until pH, 70 ℃ of oven dry, porphyrize sieves with 10000 eye mesh screens, promptly get luminescent powder, get 10% luminescent powder and epoxy resin and mix, after solidifying in 24 hours, being coated with methyl methacrylate is overcoat.
2) pearl nuclear is handled:
In the aseptic operating desk of washing one's face, with seawater through 0.45 μ m membrane filtration, add algal polysaccharides 1% by weight percentage, pack behind the abundant mixing of swelling, add chlortetracycline concentration during nuclear to be inserted to every milliliter of 1000 SI units, will the good pearl nuclear of coating in this solution, soak after 5 minutes and take out.
3) the cell small pieces are handled:
With distilled water is solvent, by weight disposing 0.6% bacillus subtilis neutral proteinase, 1.2% trypsase respectively, and 0.4% pepsin, with the homogenate of thawing of feed shellfish (freshwater mussel) meat, solid-liquid ratio is 1: 1: 1: 1.Feed shellfish (freshwater mussel) meat of homogenate of thawing adds bacillus subtilis neutral proteinase solution earlier, regulates pH to 8.0,50 ℃ of temperature, hydrolysis 4 hours; PH to 6.5 is regulated in the back, and 40 ℃ of temperature add trypsin solution, hydrolysis 4 hours; After add pepsin again and regulate pH to 3.0,60 ℃ of temperature, hydrolysis 4 hours; The active carbon of adding 1.5%: diatomite was handled 50 minutes for 60 ℃ by the decolorizer of weight ratio mixing in 2: 3; Pack, add aureomycin during nuclear to be inserted, concentration reaches every milliliter of 1000 SI units, the cell small pieces is soaked in this solution take out nuclear use to be inserted after 1 minute.
When pearl nuclear is implanted in operation, with opener the pteria martensii shell is opened, with chock bolt mouth, open a kerf with recessing tool at foot and visceral mass intersection, insert from otch with access needle, tiltedly stretch, pass from the internal sac epidermis below and the sufficient flesh top of contracting to abdomen ridge direction, go back to the place ahead at bent place to abdomen ridge enteron aisle, promptly passage is finished; With nucleus sending device the pearl nuclear of handling well is sent into the wound place again, the back all is close to treated cell small pieces on the pearl nuclear with the small pieces pricker, after the operation shellfish put into to hang in the storage pond, holding pond and support recuperation in 20 days, it is pure and fresh that the storage pond, holding pond water quality requirement keeps, bait is abundant, after put back to and culture the waters and hang and supported 1 year, ripe results get final product night the fluorescence pearl.
Embodiment 2
The crown freshwater mussel of cultivation pleat line fluorescence at night pearl shell can be divided into following steps to carry out:
1) luminescent powder coating pearl nuclear:
With Al
2O
3, Al (OH)
3, Eu
2O
34: 1: 4 in molar ratio, add 1% (by weight percentage) H again
3BO
3As cosolvent, the abundant mix grinding of above raw material is even, 1400 ℃ of sintering, sintering time is 2.5 hours, takes out and the pulverizing intermediate product, and is synthetic in 1500 ℃ of nitrogen atmospheres, take out to pulverize the back again with 1% KOH solution washing,, sample is filtered through filter paper until pH to 10.0,70 ℃ of oven dry, porphyrize sieves with 10000 eye mesh screens, promptly get luminescent powder, get 10% luminescent powder and epoxy resin and mix, after solidifying in 24 hours, being coated with methyl methacrylate is overcoat.
2) pearl nuclear is handled:
In the aseptic operating desk of washing one's face, seawater through 0.45 μ m membrane filtration, is added algal polysaccharides 2% by weight percentage, pack behind the abundant mixing of swelling, add every milliliter of chlortetracycline concentration 2000 SI units during nuclear to be inserted, will the good pearl nuclear of coating in this solution, soak after 10 minutes and take out.
3) the cell small pieces are handled:
With distilled water is solvent, by weight disposing 0.9% bacillus subtilis neutral proteinase solution, 1.7% trypsin solution respectively, and 0.6% pepsin solution, with the homogenate of thawing of feed shellfish (freshwater mussel) meat, solid-liquid ratio is 1: 1: 1: 1.Feed shellfish (freshwater mussel) meat of homogenate of thawing adds bacillus subtilis neutral proteinase solution earlier, regulates pH to 8.0,50 ℃ of temperature, hydrolysis 4 hours; PH to 6.5 is regulated in the back, and 40 ℃ of temperature add trypsin solution, hydrolysis 4 hours; After add pepsin again and regulate pH to 3.0,60 ℃ of temperature, hydrolysis 4 hours; The active carbon of adding 2%: diatomite was handled 50 minutes for 60 ℃ by the decolorizer of weight ratio mixing in 2: 3; Pack, add aureomycin during nuclear to be inserted, concentration reaches every milliliter of 2000 SI units, the cell small pieces is soaked in this solution take out nuclear use to be inserted after 1 minute.
When pearl nuclear is implanted in operation, the crown freshwater mussel shell of pleat line is opened, with chock bolt mouth with opener, the back is pushed the shell edge central authorities overcoat putamina flesh of front end partially aside with the wound plate, hand takes common pearl nuclear or moulding pearl nuclear to send into the wound place, again with nucleus sending device with pearl nuclear propulsion housing central authorities, postoperative freshwater mussel body wound is downward, put into to hang in the storage pond, holding pond to support and be the recuperation phase in 20 days, it is pure and fresh that the storage pond, holding pond water quality requirement keeps, and bait is abundant, after put back to and culture the waters and hang foster, grew cultured pearls 1 year, ripe results get final product night the fluorescence pearl.
Claims (4)
- One kind night the fluorescence pearl shell breeding method, it is characterized in that: take from the system luminescent powder and mix by 10%-15% weight ratio and epoxy resin, be coated in pearl nuclear surface, after curing in 20-24 hour, being coated with methyl methacrylate is overcoat; Pearl nuclear was soaked 5-10 minute in containing the pearl nuclear conditioning agent of algal polysaccharides and aureomycin, the cell small pieces soak after 1-2 minute in the cell small pieces conditioning agent that contains aureomycin and shellfish body hydrolyzate, insert the nuclear operation again; After the operation freshwater mussel or pearly shellfish are supported temporarily in the recuperation pond, after put back to and culture the waters and hang fosterly, obtain fluorescence pearl shell at night.
- 2. according to the breeding method of claim 1 fluorescence pearl shell at described a kind of night, it is characterized in that: the preparation method of described self-control luminescent powder is: mol ratio is 4: 1: 4 Al 2O 3, Al (OH) 3And Eu 2O 3Mixture, add 1%-3% weight H 3BO 3As cosolvent, fully mix grinding is even, and 1400 ℃ of sintering, sintering time are 2.5-3 hour; Take out and also to pulverize intermediate product, sintering in 1500 ℃ of nitrogen, taking out and pulverizing the back is the KOH solution washing of 1%-3% with concentration, until pH less than 10.5; Filter the 70-80 ℃ of oven dry in back, porphyrize with the screening of 10000 eye mesh screens, promptly gets luminescent powder.
- 3. according to the breeding method of claim 1 fluorescence pearl shell at described a kind of night, it is characterized in that: the preparation method of described pearl nuclear conditioning agent is: in the aseptic operating desk of washing one's face with seawater through 0.45 μ m membrane filtration, press the 1%-2% weight ratio and add algal polysaccharides, carry out packing behind the swelling mixing, add aureomycin during nuclear to be inserted and make its concentration reach every milliliter of 1000-2000 SI units, promptly get pearl and examine conditioning agent.
- 4. according to the breeding method of claim 1 fluorescence pearl shell at described a kind of night, it is characterized in that: the preparation method of described cell small pieces conditioning agent is: with distilled water be feed shellfish, freshwater mussel meat after 0.6%-0.9% bacillus subtilis neutral proteinase solution, 1.2%-1.7% trypsin solution, 0.4%-0.8% pepsin solution and the homogenate of thawing of solvent by 1: 1: 1: 1 weight ratio is prepared; Shellfish, freshwater mussel meat thaw and regulate pH to 8.0-8.5 after the homogenate, and 40 ℃-50 ℃ of temperature add bacillus subtilis neutral proteinase solution, hydrolysis 3.5-4 hour; Regulate pH to 6-6.5 afterwards, 35 ℃-40 ℃ of temperature add trypsin solution, hydrolysis 3-4 hour; Regulate pH to 3.0 afterwards, 55 ℃-60 ℃ of temperature add pepsin solution, hydrolysis 3-4 hour again; Add the decolorizer that 1.5%-2% mixes by weight with active carbon and diatomite at 2: 3 then, handle after 50-70 minute for 60-70 ℃ and pack, add aureomycin during nuclear to be inserted and make its concentration reach every milliliter of 1000-2000 SI units, promptly get cell small pieces conditioning agent.
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| CN2011101175527A CN102246715A (en) | 2011-04-29 | 2011-04-29 | Breeding method of night fluorescent pearl oyster |
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|---|---|---|---|
| CN2011101175527A CN102246715A (en) | 2011-04-29 | 2011-04-29 | Breeding method of night fluorescent pearl oyster |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283661A (en) * | 2013-06-26 | 2013-09-11 | 千足珍珠集团股份有限公司 | Nucleated pearl lamella conditioning fluid and application thereof |
| CN103299940A (en) * | 2013-06-26 | 2013-09-18 | 千足珍珠集团股份有限公司 | Method for culturing nucleated pearls |
| CN103355229A (en) * | 2013-06-06 | 2013-10-23 | 金华职业技术学院 | Immunostimulation generation method of pearl sac of pearl mussel |
| CN103404460A (en) * | 2013-08-13 | 2013-11-27 | 北海市珍源海洋生物有限公司 | Cultivation raw material and method for black rose pearl growing factors |
| CN103918582A (en) * | 2013-01-10 | 2014-07-16 | 王俊杰 | Pearl nucleus for pearl culture and production method thereof |
| CN104737954A (en) * | 2015-04-09 | 2015-07-01 | 广东海洋大学 | Super-green seawater pearl cultivation method |
| CN104839057A (en) * | 2015-05-20 | 2015-08-19 | 广西南珠宫投资控股集团有限公司 | Method of cultivating Pinctada martensii to produce luminous pearls |
| CN104855315A (en) * | 2015-05-20 | 2015-08-26 | 广西南珠宫投资控股集团有限公司 | Culture method for colored anion pearls |
| CN112655614A (en) * | 2020-12-25 | 2021-04-16 | 谢绍河 | Novel high-grade amber beeswax pearl and production method thereof |
| CN113207768A (en) * | 2021-06-07 | 2021-08-06 | 北部湾大学 | Novel method for cultivating free pearls of pteria penguin |
| CN113207769A (en) * | 2021-06-07 | 2021-08-06 | 北部湾大学 | Novel method for cultivating large free pearls by using pinctada maxima |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103918582A (en) * | 2013-01-10 | 2014-07-16 | 王俊杰 | Pearl nucleus for pearl culture and production method thereof |
| CN103355229A (en) * | 2013-06-06 | 2013-10-23 | 金华职业技术学院 | Immunostimulation generation method of pearl sac of pearl mussel |
| CN103355229B (en) * | 2013-06-06 | 2015-01-07 | 金华职业技术学院 | Immunostimulation generation method of pearl sac of pearl mussel |
| CN103299940A (en) * | 2013-06-26 | 2013-09-18 | 千足珍珠集团股份有限公司 | Method for culturing nucleated pearls |
| CN103299940B (en) * | 2013-06-26 | 2014-09-03 | 千足珍珠集团股份有限公司 | Method for culturing nucleated pearls |
| CN103283661A (en) * | 2013-06-26 | 2013-09-11 | 千足珍珠集团股份有限公司 | Nucleated pearl lamella conditioning fluid and application thereof |
| CN103404460A (en) * | 2013-08-13 | 2013-11-27 | 北海市珍源海洋生物有限公司 | Cultivation raw material and method for black rose pearl growing factors |
| CN104737954B (en) * | 2015-04-09 | 2017-09-29 | 广东海洋大学 | A kind of breeding method of emerald green sea water pearls |
| CN104737954A (en) * | 2015-04-09 | 2015-07-01 | 广东海洋大学 | Super-green seawater pearl cultivation method |
| CN104839057A (en) * | 2015-05-20 | 2015-08-19 | 广西南珠宫投资控股集团有限公司 | Method of cultivating Pinctada martensii to produce luminous pearls |
| CN104855315B (en) * | 2015-05-20 | 2017-06-30 | 广西南珠宫投资控股集团有限公司 | A kind of cultural method of colored anion pearl |
| CN104855315A (en) * | 2015-05-20 | 2015-08-26 | 广西南珠宫投资控股集团有限公司 | Culture method for colored anion pearls |
| CN112655614A (en) * | 2020-12-25 | 2021-04-16 | 谢绍河 | Novel high-grade amber beeswax pearl and production method thereof |
| CN113207768A (en) * | 2021-06-07 | 2021-08-06 | 北部湾大学 | Novel method for cultivating free pearls of pteria penguin |
| CN113207769A (en) * | 2021-06-07 | 2021-08-06 | 北部湾大学 | Novel method for cultivating large free pearls by using pinctada maxima |
| CN113207768B (en) * | 2021-06-07 | 2022-11-04 | 北部湾大学 | Novel method for cultivating free pearls of pteria penguin |
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Application publication date: 20111123 |