CN103243053A - Fermentative bacteria solution for peanut vines and peanut vine fermentation feed - Google Patents
Fermentative bacteria solution for peanut vines and peanut vine fermentation feed Download PDFInfo
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- CN103243053A CN103243053A CN2013101936531A CN201310193653A CN103243053A CN 103243053 A CN103243053 A CN 103243053A CN 2013101936531 A CN2013101936531 A CN 2013101936531A CN 201310193653 A CN201310193653 A CN 201310193653A CN 103243053 A CN103243053 A CN 103243053A
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Abstract
The invention discloses a fermentative bacteria solution for peanut vines and a peanut vine fermentation feed, which belong to the technical field of fermentation feeds. The fermentative bacteria solution for peanut vines is composed of the following components in parts by weight: 30-40 parts of a bacteria solution A and 10-15 parts of a bacteria solution B, every 40g of the bacteria solution A contains cellulomonas (1*10<9>-4*10<9> CFU), butyrivibrio fibrisolvens (1.5*10<9>-5*10<9> CFU), saccharomyces cerevisiae (1.68*10<9>-4.32*10<9> CFU), bacillus subtilis (2.25*10<10>-6.67*10<10> CFU), corn steep liquor (1-3g by dry matters), and the balance of water and necessary microbial matrixes; and every 10g of bacteria solution B contains lactic acid bacteria (0.22*10<10>-0.96*10<10> CFU), corn steep liquor (0.47-1.35g by dry matters), whole milk powder (0.67-2g), and the balance of water and necessary microbial matrixes. A peanut vine fermentation feed prepared by using the fermentative bacteria solution is prepared from the following components in parts by weight through mixing and fermenting: 45-55 parts of peanut vine powder, 5-8 parts of corn flour, 2-3 parts of soybean meal powder, 1-2 parts of urea, 1-2 parts of calcium sulphate and 40-55 parts of the fermentative bacteria solution.
Description
Technical field
The invention belongs to the fermented feed technical field, the fermented feed that relates to a kind of zymocyte liquid and utilize this zymocyte liquid to make.
Background technology
The peanut seedling quality is soft and nutritive substance abundant, the equal edible of various livestock and poultry.Relevant assay determination result shows, contain in the peanut seedling cauline leaf of crawling to grow that crude protein is about 12.00%～13.50%, crude fat is about 2.00%～3.00%, carbohydrate (mainly being robust fibre) is about 46.00%～48%, wherein the gross protein value of Folium Arachidis hypogaeae is up to 20%.With regard to digestible protein, 1 kilogram of dry peanut seedling contains digestible protein 70 grams approximately, contains 17 gram calcium, 7 gram phosphorus in addition.The gross protein value of peanut seedling is 1.6 times of pea seedling, is 6 times of straw.The livestock and poultry energy that 1 kilogram of peanut seedling produces of searching for food is equivalent to the energy that 0.6 kilogram of barley produces.300 kilograms of peanuts of general every mu of real estate just can obtain 300 kilograms peanut seedling, then are equivalent to the feeding effect of 180 kilograms of barleys for the domestic animal of feeding.
Peanut seedling is inexpensive, but present utilization rate is lower, and its processing mode is still to burn also Tian Weizhu.Tracing it to its cause, mainly is because also have following defective as the feed peanut seedling: 1) crude fiber content height, and the livestock and poultry digestibility is low; 2) not anti-storage: peanut seedling stacking floor space is big, and influenced by assorted bacterium and go mouldy.
Summary of the invention
The objective of the invention is to provides a kind of pair of component zymocyte liquid at peanut seedling, and the peanut seedling fermented feed that utilizes this zymocyte liquid to make also is provided simultaneously.
Based on above-mentioned purpose, the present invention has taked following technical scheme:
The peanut seedling zymocyte liquid is made up of the A bacterium liquid of 30～40 weight parts and the B bacterium liquid of 10～15 weight parts, wherein fibre-bearing Zymomonas mobilis 1 * 10 in every 40g A bacterium liquid
9～4 * 10
9CFU, Butyrivibrio fibrisolvens 1.5 * 10
9～5 * 10
9CFU, S. cervisiae 1.68 * 10
9～4.32 * 10
9CFU, subtilis 2.25 * 10
10～6.67 * 10
10CFU, corn steep liquor are in dry-matter 1～3g, and all the other are water and necessary microorganism matrix (contained microorganism matrix in the commercial bacterium powder); Contain milk-acid bacteria 0.22 * 10 in every 10g B component
10～0.96 * 10
10CFU, corn steep liquor are in dry-matter 0.47-1.35g, whole milk powder 0.67～2g, and all the other are water and necessary microorganism matrix.
Get cellulomonas cartae preparation, Butyrivibrio fibrisolvens preparation, yeast saccharomyces cerevisiae bacteria preparation, bacillus subtilis formulation, corn steep liquor and water and mix, namely got A bacterium liquid in 21～27 hours in 25～28 ℃ of airtight placements then.
Extracting lactic acid bacteria preparation, corn steep liquor, whole milk powder and water mix, and namely get B bacterium liquid in 42～54 hours in 25～28 ℃ of airtight placements then.
The peanut seedling fermented feed that utilizes described zymocyte liquid to make, in weight part, this fermented feed is formed by 45～55 parts of peanut vine powders, 5～8 portions of Semen Maydis powder, 2～3 parts of bean cake powders, 1～2 part of urea, 1～2 part of calcium sulfate and 40～55 parts of zymocyte liquid mixing, fermentations.
Described peanut vine powder, Semen Maydis powder, bean cake powder are crossed 1.5～2.5 mm sieve.
Described fermentation is carried out in having the fermentation bag of one-way exhaust valve, and 25～28 ℃ of leavening temperatures treat that the mixture air-blowing can stop fermentation in the fermentation bag.
Provided by the inventionly can improve crude protein and the crude fat content of peanut seedling with zymocyte liquid, reduce crude fiber content, produce simultaneously and improve immunity function
Linear gramicidinsWith the multiple active ingredient that digests of helping, as vitamin B group, digestive ferment, cellulase etc.Wherein, cellulomonas cartae (Cellulomonas) can be at decomposition of cellulose under the aerobic condition, and the anerobe as Butyrivibrio fibrisolvens (Butyrivibrio fibrisolvens) then can be decomposed Mierocrystalline cellulose at anaerobic condition.S. cervisiae can suck monose such as glucose, fructose, seminose in the cell, through the effect of endoenzyme, the monose fermentation is decomposed into carbonic acid gas and alcohol under oxygen free condition.Yeast rich vitamin B, protein and plurality of enzymes help digesting and assimilating of nutrient.Subtilis
ThallineThe subtilyne that in process of growth, produces, polymyxin, nystatin,
Linear gramicidinsThe isoreactivity material to pathogenic bacterium or
Autogenous infectionConditioned pathogen the obvious suppression effect is arranged.Simultaneously, subtilis also can consume the free oxygen in the environment rapidly, further promotes useful
AnerobeGrowth, and produce lactic acid etc.
Organic acidClass reduces enteron aisle pH value, suppresses other pathogenic bacterium growth indirectly.Subtilis thalline self synthesize α-Dian Fenmei,
Proteolytic enzyme, lipase,
Mierocrystalline celluloseEnzymes such as enzyme can synthesize
Vitamins B 1 , B
2, B
6, multiple vitamin B group such as nicotinic acid, and improve in the animal body
Interferon, rabbitActivity with scavenger cell.Replenish the effect of some milk-acid bacterias
Peanut seedling fermented feed provided by the invention is core material with the peanut seedling, is aided with Semen Maydis powder, bean cake powder, urea and calcium sulfate, can ferment behind the inoculation fermentation liquid, just can obtain ideal product usually after 24～48 hours.Test-results shows, crude fiber content reduces in this fermented feed, histone matter content improves, the palatability of feed is improved, in middle hog grower feed, add 5～10% the alternative equivalent wheat bran of peanut seedling fermented feed, can promote pork pig to grow, reduce the pork pig aquaculture cost, have good market popularization value.
Embodiment
The present invention will be further described below in conjunction with specific embodiment.
Embodiment 1
The peanut seedling zymocyte liquid is made up of 30 kgA bacterium liquid and 10kg B bacterium liquid.
Fibre-bearing Zymomonas mobilis 4 * 10 in every 40g A bacterium liquid wherein
9CFU, Butyrivibrio fibrisolvens 5 * 10
9CFU, S. cervisiae 4.32 * 10
9CFU, subtilis 6.67 * 10
10CFU, corn steep liquor are in dry-matter 3g, and all the other are water and necessary microorganism matrix.Get 1.0 kg cellulomonas cartae solid preparation (viable count content 3.0 * 10
9CFU/g), 2.5 kg Butyrivibrio fibrisolvens solid preparation (viable count content 1.5 * 10
9CFU/g), 1.8 kg S. cervisiae solid preparation (viable count content 1.8 * 10
9CFU/g), 2.0 kg subtilis solid preparation (viable count content 2.5 * 10
10CFU/g), 5kg corn steep liquor (dry matter content 45wt%) mixes, and adds water and supplies 30kg, namely gets A bacterium liquid in 21 hours in 25～28 ℃ of airtight placements then.
Contain milk-acid bacteria 0.96 * 10 in every 10g B component
10CFU, corn steep liquor are in dry-matter 1.35g, whole milk powder 2g, and all the other are water and necessary microorganism matrix.Get 0.6kg milk-acid bacteria solid preparation (viable count content 1.6 * 10
10CFU/g), 3kg corn steep liquor (dry matter content 45wt%), 2kg whole milk powder mix, and adds water and supply 10kg, mix the back and namely got B bacterium liquid in 42 hours in 25～28 ℃ of airtight placements.
The peanut seedling fermented feed is formed by 45kg peanut vine powder, 5 kg Semen Maydis powder, 2kg bean cake powder, 1 kg urea, 1 kg calcium sulfate and the mixing of 40 kg zymocyte liquids, fermentation; Peanut vine powder, Semen Maydis powder, bean cake powder are crossed 1.5 mm sieve; Fermenting process carries out in having the fermentation bag of one-way exhaust valve, and 25 ℃ of leavening temperatures treat that the mixture air-blowing can stop fermentation in the fermentation bag.
Embodiment 2
The peanut seedling zymocyte liquid is made up of 40 kg A bacterium liquid and 15 kg B bacterium liquid.
Fibre-bearing Zymomonas mobilis 1 * 10 in every 40g A bacterium liquid wherein
9CFU, Butyrivibrio fibrisolvens 1.5 * 10
9CFU, S. cervisiae 1.68 * 10
9CFU, subtilis 2.25 * 10
10CFU, corn steep liquor are in dry-matter 1 g, and all the other are water and necessary microorganism matrix.Get 0.5kg cellulomonas cartae solid preparation (viable count content 2.0 * 10
9CFU/g), 1.5 kg Butyrivibrio fibrisolvens solid preparation (viable count content 1.0 * 10
9CFU/g), 1.2kg S. cervisiae solid preparation (viable count content 1.4 * 10
9CFU/g), 1.5kg subtilis solid preparation (viable count content 1.5 * 10
10CFU/g), 3kg corn steep liquor (dry matter content 34wt%), mix and add water and supply 40kg, mix the back namely got A bacterium liquid in 27 hours in 25～28 ℃ of airtight placements.
Contain milk-acid bacteria 0.22 * 10 in every 10g B component
10CFU, corn steep liquor are in dry-matter 0.47g, whole milk powder 0.67g, and all the other are water and necessary microorganism matrix.Take by weighing 0.3kg lactic acid bacteria formulation (viable count content 1.1 * 10
10CFU/g), 2kg corn steep liquor (dry matter content 35wt%), 1kg whole milk powder mix, and adds water and supply 15kg, mix the back and namely got B bacterium liquid in 54 hours in 25～28 ℃ of airtight placements.
The peanut seedling fermented feed is formed by 55 kg peanut vine powders, 8 kg Semen Maydis powder, 3 kg bean cake powders, 2 kg urea, 2 kg calcium sulfate and the mixing of 55 kg zymocyte liquids, fermentation; Peanut vine powder, Semen Maydis powder, bean cake powder are crossed 2.5 mm sieve; Fermenting process carries out in having the fermentation bag of one-way exhaust valve, and 28 ℃ of leavening temperatures treat that the mixture air-blowing can stop fermentation in the fermentation bag.
Embodiment 3
The peanut seedling zymocyte liquid is made up of 35 kg A bacterium liquid and 12 kg B bacterium liquid.
Fibre-bearing Zymomonas mobilis 2 * 10 in every 40g A bacterium liquid wherein
9CFU, Butyrivibrio fibrisolvens 3 * 10
9CFU, S. cervisiae 3 * 10
9CFU, subtilis 4 * 10
10CFU, corn steep liquor are in dry-matter 2g, and all the other are water and necessary microorganism matrix.Take by weighing cellulomonas cartae solid preparation (2.5 * 10 in proportion
9CFU/g), the Butyrivibrio fibrisolvens solid preparation (1.2 * 10
9CFU/g), the S. cervisiae solid preparation (1.6 * 10
9CFU/g), the subtilis solid preparation (2.0 * 10
10CFU/g), corn steep liquor (dry matter content 40wt%) mixes, and adds water and supplies 35kg, mix the back and namely got A bacterium liquid in 24 hours in 25～28 ℃ of airtight placements.
Contain milk-acid bacteria 0.5 * 10 in every 10g B component
10CFU, corn steep liquor are in dry-matter 0.8g, whole milk powder 1.5g, and all the other are water and necessary microorganism matrix.Take by weighing milk-acid bacteria solid preparation (1.2 * 10 in proportion
10CFU/g), corn steep liquor (dry matter content 40wt%), whole milk powder mix, and adds water and supply 12kg, mix the back and namely got B bacterium liquid in 48 hours in 25～28 ℃ of airtight placements.
The peanut seedling fermented feed is formed by 50 kg peanut vine powders, 6 kg Semen Maydis powder, 2.5 kg bean cake powders, 1.6 kg urea, 1.5 kg calcium sulfate and the mixing of 47 kg zymocyte liquids, fermentation; Peanut vine powder, Semen Maydis powder, bean cake powder are crossed 2.0 mm sieve; Fermenting process carries out in having the fermentation bag of one-way exhaust valve, and 26 ℃ of leavening temperatures treat that the mixture air-blowing can stop fermentation in the fermentation bag.
Embodiment 4
Press the feed sample method of sampling, not 6 parts of fermenting peanut seedling mixture and fermenting peanut seedling blend sample of random acquisition from embodiment 1-3 respectively, according to crude protein, crude fat, crude fiber content in the conventional nutrient analysis method of the feed working sample, measurement result sees Table 1.As can be seen from Table 1, improve crude protein and crude fat content after the peanut seedling fermented mixture, reduced crude fiber content.
The variation of component content before and after the table 1 peanut seedling fermented mixture
| ? | Crude protein | Crude fat | Robust fibre |
| Fermenting peanut seedling mixture % not | 12.62±1.02 a | 2.21±0.12 a | 46.52±4.25 a |
| Fermenting peanut seedling mixture % | 15.81±1.14 b | 2.84±0.11 b | 40.22±4.12 b |
| Rangeability | +25.28% | +28.51% | -13.54% |
Annotate: the identical English alphabet of same column represents difference, and significantly (P 〉=0.05), different English alphabet do not represent significant difference (P≤0.05).
The test of embodiment 5 feeding effects
5.1 test method
Select 540 of the DLY three way cross growing swine of 65 ages in days, mean body weight 25.12kg(25.12 ± 0.50kg); The principle that approaches according to each group male and female number only is divided into 9 treatment group with test pig, 6 repetitions of each treatment group, and each repeats 10.9 treatment group are designated as 1 group of contrast, 2 groups of contrasts, 3 groups of contrasts, test 1-6 group respectively.Each organizes a pig daily ration proportioning referring to table 2, and day feeds three times, freely drinks water.Test period is 90 days.
Each test group pig of table 2 daily ration proportioning (weight ratio adds up to 1000)
| Numbering | Corn | Dregs of beans | Wheat bran | Fish meal | Fermenting peanut seedling mixture not | Fermenting peanut seedling mixture |
| Contrast 1 group | 630 | 240 | 110 | 20 | - | - |
| Contrast 2 groups | 630 | 240 | 50 | 20 | 60 | - |
| Contrast 3 groups | 630 | 240 | 30 | 20 | 80 | - |
| Test 1 group | 630 | 240 | 50 | 20 | - | 60 parts of embodiment 1 products |
| Test 2 groups | 630 | 240 | 30 | 20 | - | 80 parts of embodiment 1 products |
| Test 3 groups | 630 | 240 | 50 | 20 | - | 60 parts of embodiment 2 products |
| Test 4 groups | 630 | 240 | 30 | 20 | - | 80 parts of embodiment 2 products |
| Test 5 groups | 630 | 240 | 50 | 20 | - | 60 parts of embodiment 3 products |
| Test 6 groups | 630 | 240 | 30 | 20 | - | 80 parts of embodiment 3 products |
5.2 test-results
By table 3, table 4, table 5 result as can be seen, compare for 1 group with contrast, add 6%, 8% not fermenting peanut seedling mixture replacing wheat bran in the daily ration after, reduce day weight gain and the food consumption of growing swine fattening pig, improve feedstuff-meat ratio; With 1 group of contrast and not fermenting peanut seedling mixture group compare, add 6%, 8% embodiment 1,2,3 fermenting peanut seedling mixture replacing wheat bran in the daily ration after, obviously improve day weight gain and the food consumption of growing swine fattening pig, the reduction feedstuff-meat ratio.
Table 3 fermenting peanut seedling is to the influence (test period 1-45d) of store pig production performance
Table 4 fermenting peanut seedling is to the influence (test period 46-90d) of store pig production performance
Table 5 fermenting peanut seedling is to the influence (test period 1-90d) of store pig production performance
5.3 interpretation of result
Found out by table 6, table 7 interpretation of result, compare for 1 group with contrast, add 6%, 8% not fermenting peanut seedling mixture replacing wheat bran in the daily ration after, obviously reduce day weight gain and the food consumption in each stage of growing swine fattening pig, improve feedstuff-meat ratio; With 1 group of contrast and not fermenting peanut seedling mixture group compare, add 6%, 8% embodiment 1,2,3 fermenting peanut seedling mixture replacing wheat bran in the daily ration after, obviously improve day weight gain and the food consumption in each stage of growing swine fattening pig, the reduction feedstuff-meat ratio.Test-results shows: it is production performance that fermenting peanut seedling mixture can obviously improve store pig, can significantly promote store pig growth and feed conversion, improves day weight gain, reduces feedstuff-meat ratio.
Table 6 test group is compared % with not substituting wheat bran (contrasting 1 group)
Table 7 test group with add not the control group of fermenting peanut seedling mixture (mean value) % that compares
Claims (6)
1. the peanut seedling zymocyte liquid is made up of the A bacterium liquid of 30～40 weight parts and the B bacterium liquid of 10～15 weight parts, wherein fibre-bearing Zymomonas mobilis 1 * 10 in every 40g A bacterium liquid
9～4 * 10
9CFU, Butyrivibrio fibrisolvens 1.5 * 10
9～5 * 10
9CFU, S. cervisiae 1.68 * 10
9～4.32 * 10
9CFU, subtilis 2.25 * 10
10～6.67 * 10
10CFU, corn steep liquor are in dry-matter 1～3g, and all the other are water and necessary microorganism matrix; Contain milk-acid bacteria 0.22 * 10 in every 10g B component
10～0.96 * 10
10CFU, corn steep liquor are in dry-matter 0.47-1.35g, whole milk powder 0.67～2g, and all the other are water and necessary microorganism matrix.
2. peanut seedling zymocyte liquid as claimed in claim 1, it is characterized in that, get cellulomonas cartae preparation, Butyrivibrio fibrisolvens preparation, yeast saccharomyces cerevisiae bacteria preparation, bacillus subtilis formulation, corn steep liquor and water and mix, namely got A bacterium liquid in 21～27 hours in 25～28 ℃ of airtight placements then.
3. peanut seedling zymocyte liquid as claimed in claim 1 is characterized in that, extracting lactic acid bacteria preparation, corn steep liquor, whole milk powder and water mix, and namely gets B bacterium liquid in 42～54 hours in 25～28 ℃ of airtight placements then.
4. the peanut seedling fermented feed that utilizes the arbitrary described zymocyte liquid of claim 1-3 to make, it is characterized in that, in weight part, described fermented feed is formed by 45～55 parts of peanut vine powders, 5～8 portions of Semen Maydis powder, 2～3 parts of bean cake powders, 1～2 part of urea, 1～2 part of calcium sulfate and 40～55 parts of zymocyte liquid mixing, fermentations.
5. peanut seedling fermented feed as claimed in claim 4 is characterized in that, described peanut vine powder, Semen Maydis powder, bean cake powder are crossed 1.5～2.5 mm sieve.
6. peanut seedling fermented feed as claimed in claim 5 is characterized in that, described fermentation is carried out in having the fermentation bag of one-way exhaust valve, and 25～28 ℃ of leavening temperatures treat that the mixture air-blowing can stop fermentation in the fermentation bag.
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| CN105076892A (en) * | 2015-09-23 | 2015-11-25 | 安徽省兴源草业有限公司 | Sweet potato vine fermented feed for sows |
| CN107494919A (en) * | 2017-10-11 | 2017-12-22 | 安徽省宿州市民辉养殖有限公司 | Big pig's feed and preparation method thereof in one kind |
| CN107712271A (en) * | 2017-10-23 | 2018-02-23 | 江苏棉海园林工程有限公司 | A kind of preparation method and its leavening of fermenting peanut seedling |
| CN110338269A (en) * | 2019-08-09 | 2019-10-18 | 刘建军 | A method of nonreactive mutton sheep TMR particulate material is produced using peanut vine |
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| CN103243053B (en) | 2014-08-13 |
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