CN1608486A - Microbial fermented feed, microbe mother liquor for fermented feed and its production process - Google Patents
Microbial fermented feed, microbe mother liquor for fermented feed and its production process Download PDFInfo
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- CN1608486A CN1608486A CNA2004100729959A CN200410072995A CN1608486A CN 1608486 A CN1608486 A CN 1608486A CN A2004100729959 A CNA2004100729959 A CN A2004100729959A CN 200410072995 A CN200410072995 A CN 200410072995A CN 1608486 A CN1608486 A CN 1608486A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention is microbial fermented feeds, microbe mother liquor for fermented feeds and their production process. Several microbe strains may be used in anaerobic fermentation of various crop stalk powder, husk, bran, bean residue, etc. to produce various kinds of fermented feed. The present invention has reasonable composition, scientific production process, wide application range and other advantages, and the fermented feed has low cost high feeding efficiency and capability of raising animal's resistance.
Description
Technical field
The present invention relates to a kind of animal feed, relate in particular to one kind of multiple microbial fermentation concentrated feeds and ferment essence feed microbe mother liquor and preparation method.
Background technology
At present, the raise livestock concentrated feed refers generally to the feed general designation concentrated feed less than 18% (CF/DM＜18%) of crude fiber content in the dry, and concentrated feed is divided into energy feed and protein supplement again.The dry crude protein content claims energy feed less than 20% concentrated feed, and crude protein is called protein supplement greater than 20% concentrated feed.Concentrated feed mainly contains three kinds of cerealses, bran, puffed soybean, wherein cereals: refer to the seed of cereal crops, as Chinese sorghum, barley, oat, corn, paddy etc.; Bran: referring to the byproduct of various grain processings, is energy feed as wheat bran, maize peel, Chinese sorghum chaff, rice bran etc. for bran; Puffed soybean: the byproduct of various oil crops processing such as soya-bean cake, peanut cake, rape cake, cottonseed cake, oil cake of flax seed, sunflower seed cake, maize germ cake etc. are puffed soybean, except that maize germ cake is energy feed, all belong to protein supplement.Concentrated feed then can improve feeding cost as the direct nutrition purposes, especially for feeding animals of feed, directly feeds with concentrated feed concerning the institute domesticated animal, and domesticated animal can not absorb the nutrient in the concentrated feed fully.
Summary of the invention
Main purpose of the present invention is to add the part powder of straw in concentrated feed, and increase the feed enzyme composition of concentrated feed by microbial fermentation, concentrated feed reduces the cost of concentrated feed behind multiple microbial fermentation, improve efficiency of feed utilization and nutritive value, save food, provide the multiple microorganism of a kind of employing to cooperate concentrated feeds such as fermentation cereals, bran, dregs of beans class, can produce various livestock and poultry (ox, sheep, pig, chicken, goose etc.) feed, and have multiple microbial fermentation concentrated feed and ferment essence feed microbe mother liquor and the preparation method that strengthens the disease-resistant effect of livestock and poultry.
The technical solution adopted for the present invention to solve the technical problems is:
The present invention uses many bacterial strains to cooperate anaerobic fermentation feed method, various crop material powder (corn, wheat, paddy rice, Chinese sorghum and peanut seedling, sweet potato seedling etc.) and cereals, bran, puffed soybean concentrate mixture can ferment, and can produce multiple poultry, fowl concentrated feed (ox, sheep, pig, chicken, goose), particularly make powder of straw be converted into the feed of non-ruminant animal, and have the effect that strengthens poultry, fowl premunition.
The culture medium of used bacterial classification of the present invention and the corresponding use of bacterial classification sees the following form:
Table 1
| Sequence number | Bacterium name (Chinese, Latin) | Numbering | Slant medium |
| ????1 | Produce yellowish fiber monad Cellulomonas flanigena | ?ACCC11055 | Peptone 1%, glucose 0.5%, yeast extract 0.5%, pH7.0 |
| ????2 | Bacillus amyloliquefaciens Bacillus amyloliquefaciens | ?AS1.1226??? | Peptone 1%, beef extract 0.5%, sodium chloride 0.5%, pH7.2-7.4 |
| ????3 | Bacillus coagulans Bacillus coagulans | ??IFFI10067 | Peptone 1%, beef extract 0.5%, sodium chloride 0.5%, pH7.2-7.4 |
| ????4 | Lactobacillus acidophilus Lactobacillus acidophilus | ??IFFI06005 | Skimmed milk power 10%, pH6.0 |
| ????5 | Bafillus natto Bacillus nato | ??IFFI10023 | Peptone 1%, beef extract 0.5%, sodium chloride 0.5%, pH7.2-7.4 |
| ????6 | Propionibacterium freudenreichii Propionibacterium freudenreichii | ??IFFI10019 | Peptone 1%, sucrose 2%, yeast extract 1.0%, ammonium phosphate 0.5%, pH7.2-7.4 |
| ????7 | Streptococcus fecalis Streptococcus fecalis | ??AS1.997 | Peptone 1%, sucrose 2%, yeast extract 1.0%, ammonium phosphate 0.5%, pH7.2-7.4 |
| ????8 | Trichoderma viride Trichoderma viride | ??AS3.3711 | Potato culture |
| ????9 | Geotrichum candidum Geotrichum candidum | ??AS2.1035 | Potato culture |
| ???10 | Aspergillus oryzae Aspergillous oryzae | ??IFFI02011 | Potato culture |
| ???11 | S. cervisiae Saccharomyces sp | ??AS2.1151 | The YEPD culture medium |
| ???12 | Candida utili Candida utilis | ??AS2.120 | The YEPD culture medium |
| ???13 | Fan Shi kluyveromyces Klutveromyces vanudenii | ??AS2.1591 | The YEPD culture medium |
The cereals concentrated feed comprises that following component forms by weight proportion per ton: corn 44-64%, dregs of beans 11-25%, wheat bran 11-23%, salt 1-3%, stone flour 1.5-5.5%, hay powder 3-9%, powder of straw 2-8%.
Cereals ferment essence feed microbe mother liquor selects for use bacterial classification to comprise that following component forms by weight proportion per ton: produce yellowish fiber monad 0.5-1 part, bacillus amyloliquefaciens 0.5-1.5 part, bacillus coagulans 0.5-1.5 part, lactobacillus acidophilus 0.5-1.5 part, propionibacterium freudenreichii 1.5-3.5 part, streptococcus fecalis 1-3 part, Trichoderma viride 0.5-1.5 part, aspergillus oryzae 0.5-1.5 part, S. cervisiae 0.1-0.3 part, candida utili 0.5-1.5 part and geotrichum candidum 0.5-1.5 part.
The microorganism fungus kind of selecting for use by proportioning is: produce yellowish fiber monad, bacillus amyloliquefaciens, bacillus coagulans, lactobacillus acidophilus, propionibacterium freudenreichii, streptococcus fecalis, Trichoderma viride, aspergillus oryzae, S. cervisiae, candida utili and geotrichum candidum, the method for preparing cereals ferment essence feed microbe mother liquor is as follows:
Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary cultivation → fermentation tank mixes or single strain pure culture → bacterial classification makes up → vacuum packaging → finished product in proportion.
Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3% cultivates 19-30h.
Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m
3/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-72 hour.
Bacterium Mixed culture: produce yellowish fiber monad 0.5-1 part, bacillus amyloliquefaciens 0.5-1.5 part and streptococcus fecalis 1-3 part and inoculate Mixed culture in proportion.
The Mixed culture bacteria culture media is (g/L) by weight: glucose 5g-15g, beef extract 6g-12g, (NH
4)
2SO
45g-11g and MgSO
4.7H
2O0.5-2.5g.
The independent pure culture of bacillus coagulans, lactobacillus acidophilus and propionibacterium freudenreichii.
Yeast Mixed culture: get S. cervisiae 0.1-0.3 part, candida utili 0.5-1.5 part and geotrichum candidum 0.5-1.5 part and inoculate Mixed culture in proportion.The culture medium of yeast Mixed culture is (g/L) by weight: glucose 15g-45g, dusty yeast 5g-15g, peptone 5g-15g, (NH
4)
2SO
45g-15g.
Mould Mixed culture: get Trichoderma viride 0.5-1.5 part and aspergillus oryzae 0.5-1.5 part is inoculated Mixed culture in proportion.The Mixed culture mould medium is (g/L) by weight: corn flour 15g-30g, wheat bran 5g-15g, powder of straw (5g-15g and (NH
4)
2SO
43g-9g.
The bran concentrated feed comprises that following component forms by weight proportion per ton: corn 27-47%, dregs of beans 10-24%, wheat bran 28-40%, salt 1-3%, stone flour 1.5-5.5%, hay powder 3-9%, powder of straw 2-8%.
Bran ferment essence feed microbe mother liquor selects for use bacterial classification to comprise that following component forms by weight proportion per ton: bacillus amyloliquefaciens 0.5-1.5 part, bacillus coagulans 0.5-1.5 part, lactobacillus acidophilus 0.5-1.5 part, propionibacterium freudenreichii 1.5-3.5 part, streptococcus fecalis 1.5-3.5 part, bafillus natto 0.5-1.5 part, Trichoderma viride 0.5-1.5 part, aspergillus oryzae 0.5-1.5 part, S. cervisiae 0.1-0.3 part, Fan Shi kluyveromyces 0.5-1.5 part.
The microorganism fungus kind of selecting for use by proportioning is: bacillus amyloliquefaciens, bacillus coagulans, lactobacillus acidophilus, propionibacterium freudenreichii, streptococcus fecalis, bafillus natto, Trichoderma viride, aspergillus oryzae, S. cervisiae and Fan Shi kluyveromyces, the method for preparing bran ferment essence feed microbe mother liquor is as follows:
Slant strains cultivation → liquid first order seed (shake bottle) cultivates-→ liquid secondary (seeding tank) cultivation → fermentation tank culture → bacterial classification makes up → vacuum packaging → finished product in proportion;
Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves; Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30h.
Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m
3/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-72 hour.
The bacterium Mixed culture: bacillus amyloliquefaciens 0.5-1.5 part, streptococcus fecalis 1.5-3.5 part and bafillus natto 0.5-1.5 part are inoculated Mixed culture in proportion.
Bacterium Mixed culture culture medium is (g/L) by weight: glucose 5g-15g, beef extract 6g-12g, (NH
4)
2SO
45g-11g and MgSO
4.7H
2O 0.5-2.5g.
The independent pure culture of bacillus coagulans, lactobacillus acidophilus and propionibacterium freudenreichii.
The yeast Mixed culture: S. cervisiae 0.1-0.3 part and Fan Shi kluyveromyces 0.5-1.5 part are inoculated Mixed culture according to the above ratio; Mixed culture Yeast Cultivation base is (g/L) by weight: glucose 15g-45g, dusty yeast 5g-15g, peptone 5g-15g and (NH
4)
2SO
45g-15g.
The mould Mixed culture: Trichoderma viride 0.5-1.5 part and aspergillus oryzae 0.5-1.5 part are inoculated Mixed culture in proportion.The Mixed culture mould medium is (g/L) by weight: corn flour 15g-30g, wheat bran 5g-15g, powder of straw 5g-15g (NH
4)
2SO
43g-9g.
Dregs of beans class concentrated feed comprises that following component forms by weight proportion per ton: corn 15-35%, dregs of beans 25-45%, soybean 3-13%, wheat bran 8-15%, oil cake of flax seed 7-17%, salt 1-3%, stone flour 1.5-5.5%, hay powder 3-9%, powder of straw 2-8%.
Dregs of beans class ferment essence feed microbe mother liquor selects for use bacterial classification to comprise that following component forms by dregs of beans class ferment essence feed weight proportion per ton: bacillus amyloliquefaciens 0.5-1.5 part, bacillus coagulans 0.5-1.5 part, lactobacillus acidophilus 0.5-1.5 part, propionibacterium freudenreichii 0.5-1.5 part, streptococcus fecalis 0.5-1.5 part, bafillus natto 0.5-1.5 part, Trichoderma viride 1.5-2.5 part, aspergillus oryzae 2.5-3.5 part, geotrichum candidum 0.5-1.5 part, Fan Shi kluyveromyces 0.5-1.5 part.
The microorganism fungus kind of selecting for use by proportioning is: bacillus amyloliquefaciens, bacillus coagulans, lactobacillus acidophilus, propionibacterium freudenreichii, streptococcus fecalis, bafillus natto, Trichoderma viride, aspergillus oryzae, geotrichum candidum and Fan Shi kluyveromyces, and the method for preparing dregs of beans class ferment essence feed microbe mother liquor is as follows:
Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → bacterial classification makes up → inoculates dregs of beans class concentrated feed → vacuum packaging → heap fermentation → finished product in proportion.
Slant strains is cultivated: above-mentioned bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30h.
Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m
3/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-72 hour.
The bacterium Mixed culture: bacillus amyloliquefaciens 0.5-1.5 part, streptococcus fecalis 0.5-1.5 part and bafillus natto 0.5-1.5 part are inoculated Mixed culture in proportion.
The Mixed culture bacteria culture media is (g/L) by weight: glucose 5g-15g, beef extract 6g-12g, (NH
4)
2SO
45g-11g and MgSO
4.7H
2O 0.5-2.5g.
The independent pure culture of bacillus coagulans, lactobacillus acidophilus and propionibacterium freudenreichii.
Yeast Mixed culture: get geotrichum candidum 0.5-1.5 part and Fan Shi kluyveromyces 0.5-1.5 part is inoculated Mixed culture in proportion.Mixed culture Yeast Cultivation base is (g/L) by weight: glucose 15g-45g, dusty yeast 5g-15g, peptone 5g-15g and (NH
4)
2SO
45g-15g.
The mould Mixed culture: get Trichoderma viride 1.5-3.5 part and aspergillus oryzae 1.5-3.5 part is inoculated Mixed culture in proportion, the Mixed culture mould medium is by weight (g/L): corn flour 15g-30g, wheat bran 5g-15g, powder of straw 5g-15g and (NH
4)
2SO
43g-9g.
Select cereals ferment essence feed microbe mother liquor or bran ferment essence feed microbe mother liquor or dregs of beans class ferment essence feed microbe mother liquor for use by proportioning, the method for preparing cereals ferment essence feed or bran ferment essence feed or dregs of beans class ferment essence feed is as follows:
Add corresponding microorganism mother liquor in cereals ferment essence feed or bran ferment essence feed or dregs of beans class ferment essence feed, process stirs, vacuum packaging, and apparent temperature fermentation 7-30 days obtains finished feed at last.
In cereals ferment essence feed, add cereals ferment essence feed microbe mother liquor in proportion; In bran ferment essence feed, add bran ferment essence feed microbe mother liquor in proportion; In dregs of beans class ferment essence feed, add dregs of beans class ferment essence feed microbe mother liquor in proportion.With pack into container or go into the pond and ferment of raw material, essential compacting and secluding air, apparent temperature fermentation 7-30 days, at last finished feed.
Ferment essence finished feed proterties: the color golden yellow has the fragrant and ester fragrance of strong acid, the quality softness, and palatability is strong.
Ferment essence feed proterties: fermented feed has improved the nutritional labeling of raw material, and wherein crude fibre reduces 4-6%, and thick protein improves 10-15%, and organic acid content reaches 1.6-2.0% and 70-80% is a L-lactic acid, can directly be absorbed by livestock.
Various enzyme content: cellulase 200-212 unit/g, hemicellulase 25-30 unit/g, pectase 90-117 unit/g, proteinase 9 00-1000 unit/g, beta-glucosidase 100-120 unit/g.
By to control group and the experimental group nursing that experimentizes respectively of different poultrys, bird, feed with the experiment of ferment essence feed.Feeding effect sees the following form:
The daily output (kilogram)
| Finished cattle | The little tail sheep that trembles with fear | Milk cow | Growing and fattening pigs | Laying hen | Open-minded goose (a jin/egg) | |
| Control group | ??0.975 | ??0.875 | ?20.84 | ??0.17 | (1.30 jin) | ????14-15 |
| Test group | ??1.83 | ??1.76 | ?21.9 | ??0.43 | (1.66 jin) | ?????8-10 |
The present invention meets standard GB/T6432-94 after testing; GB/T6433-94; GB/T6434-94; GB/T6436-92; GB/T6437-92; GB/T6438-92; GB/T6435-86; Wherein detect total moisture: 60.4%; Thick protein: 25.5%; Crude fat: 8.90%; Coarse ash: 13.28%; Total phosphorus: 0.19%; Calcium: 0.51%; Crude fibre; 7.12%.
The present invention is multiple microbial fermentation concentrated feed and ferment essence feed microbe mother liquor and preparation method.Reasonable recipe, the preparation method science, application region is wide, and the scope of application is extensive, and is simple to operate, grasps easily, and cost is low, and feedstuff-meat ratio is low, and the dressing percentage height is fed egg bird laying rate height.In concentrated feed, add the part powder of straw, and increase the feed enzyme composition of concentrated feed by microbial fermentation, concentrated feed reduces the cost of concentrated feed behind multiple microbial fermentation, improve efficiency of feed utilization and nutritive value, save food, adopt multiple microorganism to cooperate concentrated feeds such as fermentation cereals, bran, puffed soybean, can produce various livestock and poultry (ox, sheep, pig, chicken, goose etc.) feed, and have the disease-resistant effect of the livestock and poultry of enhancing, the present invention has following characteristics:
1, containing a large amount of digestive ferments in the concentrated feed comprises: protease, amylase, pectase, cellulase, hemicellulase, 1,4 beta-glucanase etc., by above-mentioned enzyme material hydrolysis, with cellulose in the stalk, hemicellulose, lignin etc. are partly degraded, improve the nutritive value of feed, and can make nutritional labeling enter into the digestive system of livestock, assist the digestion of livestock endogenous enzymes, directly improved digesting and assimilating of feed, in addition, can effectively reduce the viscosity of enteron aisle Dissolve things inside, prevent to stop up the intestinal cell fenestra, promote the transmission and the absorption of nutriment, improve efficiency of feed utilization;
2, the microbial cells that contains the above-mentioned digestive ferment of a large amount of secretions in the concentrated feed can be colonizated in the livestock alimentary canal, for livestock provides a large amount of digestive ferments and improves the more efficiently utilization rate that improves feed of environment in the alimentary canal;
3, have efficient cellulase-producing, hemicellulase, 1,4 beta-glucanase bacterial strain in the concentrated feed, the cellulase of its generation, hemicellulase, 1,4 beta-glucanase can be hydrolyzed into glucose with the cellulose of the straw powder that adds in the feed and hemicellulose components and be utilized by livestock, when reducing fodder cellulose content, improve feed nutrition, and save part grain;
4, contain in the concentrated feed and have acid-producing bacteria in a large number, can produce the softening stalk of a large amount of organic acids and improve palatability, the concentrated feed after the fermentation has the fragrant and ester fragrance of acid, stimulates livestock appetite, the increase feed intake.Acid can reduce pH value and suppress various spoilage organisms growths, prolongs the shelf-life of feed.The L-lactic acid that is produced is that the energy matter of ruminant directly improves its output of milk and weightening finish; Can stimulate digestion liquid secretion of lactic acid promotes gastrointestinal peristalsis, slows down gastrointestinal emptying speed, makes nutriment that digestion time more fully be arranged, and improves digestibility, and propionic acid can improve sugared content in the cow blood, reduces the toxic action of nuisance such as ketone to livestock;
5, concentrated feed contains multiple probio, is added to pasture in the feed, can improve micro-ecological environment in the livestock body, suppresses the growth of pathogenic bacteria in the livestock body, improves physique and the resistance against diseases of livestock, obviously prolongs livestock production capacity and time.
Safety non-toxic of the present invention, the adaptability of fermentation condition is strong, can both ferment under the condition of 0-35 ℃ of yeasting, and fermenting speed is directly proportional with ambient temperature, and 20 ℃ with top fermentation 7-10 days, 15 ℃ of fermentations 10-15 days.The applicable raw materials scope is wide, and the mixture of the various crop of can fermenting powder of straw and paddy reality, bran, dregs of beans is made the microbial fermentation concentrated feed that is fit to different livestocks, comprises corn, wheat, paddy rice, broomcorn straw, barley culm, grain straw, oat straw, rotten straw; The stalk of legume: big beanstalk, broad bean straw, pea straw, and the rattan of crop, climing, seedling and peanut seedling, sweet potato seedling etc.Quality softness of the present invention, loose, palatability is strong, and moisture reaches 40-60%.It is reasonable in design to fill a prescription, simple to operate, easy to make, suitable wide farmers and breed factory, the nutritional labeling that fermented feed has improved stalk wherein crude fibre reduces 4-6%, and thick protein is brought up to 10-15%, and organic acid content reaches 1.6-2.0%, and 70-80% is L-lactic acid, can directly be absorbed by livestock.The feeding effect of fermented feed is fine, and the feed palatability is better, and the digestibility of feed improves, and daily gain and meat feed ratio increase, and the livestock resistance against diseases increases.
Below in conjunction with embodiment to the detailed description of the invention.
The specific embodiment
Embodiment 1 (high dose)
The cereals concentrated feed is formed by weight ratio: corn 640kg, dregs of beans 250kg, wheat bran 230kg, salt 30kg, stone flour 55kg, hay powder 90kg, powder of straw 80kg.
Embodiment 2 (low dosage)
The cereals concentrated feed is formed by weight ratio: corn 440kg, dregs of beans 110kg, wheat bran 110kg, salt 10kg, stone flour 15kg, hay powder 30kg, powder of straw 20kg.
Embodiment 3 (medium metering)
The cereals concentrated feed is formed by weight ratio: corn 540kg, dregs of beans 180kg, wheat bran 170kg, salt 20kg, stone flour 35kg, hay powder 60kg, powder of straw 50kg.
Embodiment 4 (high dose)
Cereals concentrated feed microorganism mother liquor selects for use bacterial classification to form by every part of 1g weight proportion: produce yellowish fiber monad 1g, bacillus amyloliquefaciens 1.5g, bacillus coagulans 1.5g, lactobacillus acidophilus 1.5g, propionibacterium freudenreichii 3.5g, streptococcus fecalis 3g, Trichoderma viride 1.5g, aspergillus oryzae 1.5g, S. cervisiae 0.3g, candida utili 1.5g, geotrichum candidum 1.5g.
Embodiment 5 (low dosage)
Cereals concentrated feed microorganism mother liquor selects for use bacterial classification to form by every part of 1g weight proportion: produce yellowish fiber monad 0.5g, bacillus amyloliquefaciens 0.5g, bacillus coagulans 0.5g, lactobacillus acidophilus 0.5g, propionibacterium freudenreichii 1.5g, streptococcus fecalis 1g, Trichoderma viride 0.5g, aspergillus oryzae 0.5g, S. cervisiae 0.1g, candida utili 0.5g, geotrichum candidum 0.5g.
Embodiment 6 (medium metering)
Cereals concentrated feed microorganism mother liquor selects for use bacterial classification to form by every part of 1g weight proportion: produce yellowish fiber monad 0.75g, bacillus amyloliquefaciens 1g, bacillus coagulans 1g, lactobacillus acidophilus 1g, propionibacterium freudenreichii 2.5g, streptococcus fecalis 2g, Trichoderma viride 1g, aspergillus oryzae 1g, S. cervisiae 0.2g, candida utili 1g, geotrichum candidum 1g.
Embodiment 7 (high dose)
The microorganism fungus kind of selecting for use by proportioning is: produce yellowish fiber monad, bacillus amyloliquefaciens, bacillus coagulans, lactobacillus acidophilus, propionibacterium freudenreichii, streptococcus fecalis, Trichoderma viride, aspergillus oryzae, S. cervisiae, candida utili and geotrichum candidum, the method for preparing cereals ferment essence feed microbe mother liquor is as follows:
Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary cultivation → fermentation tank mixes or single strain pure culture → bacterial classification makes up → vacuum packaging → finished product in proportion.
Slant strains is cultivated: above-mentioned bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-1 80 times/minute, inoculum concentration 1-3% cultivates 19-30h.
Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m
3/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-72 hour.
Every part of 1g meter of bacterium Mixed culture: produce yellowish fiber monad 1g, bacillus amyloliquefaciens 1.5g and streptococcus fecalis 3g and inoculate Mixed culture in proportion.
The Mixed culture bacteria culture media is (g/L) by weight: glucose 15g, beef extract 12g, (NH
4)
2SO
411g and MgSO
4.7H
2O 2.5g.
The independent pure culture of bacillus coagulans, lactobacillus acidophilus and propionibacterium freudenreichii.
Every part of 1g meter of yeast Mixed culture: get S. cervisiae 0.3g, candida utili 1.5g and geotrichum candidum 1.5g and inoculate Mixed culture in proportion.Mixed culture Yeast Cultivation base is (g/L) by weight: glucose 45g, dusty yeast 15g, peptone 15g and (NH
4)
2SO
415g.
Every part of 1g of mould Mixed culture meter: get Trichoderma viride 1.5g and aspergillus oryzae 1.5g inoculates Mixed culture in proportion.The Mixed culture mould medium is (g/L) by weight: corn flour 30g, wheat bran 15g, powder of straw 15g and (NH
4)
2SO
49g.
Embodiment 8 (low dosage)
The microorganism fungus kind of selecting for use by proportioning is: produce yellowish fiber monad, bacillus amyloliquefaciens, bacillus coagulans, lactobacillus acidophilus, propionibacterium freudenreichii, streptococcus fecalis, Trichoderma viride, aspergillus oryzae, S. cervisiae, candida utili and geotrichum candidum, the method for preparing cereals ferment essence feed microbe mother liquor is as follows:
Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary cultivation → fermentation tank mixes or single strain pure culture → bacterial classification makes up → vacuum packaging → finished product in proportion.
Slant strains is cultivated: above-mentioned bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3% cultivates 19-30h.
Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m
3/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-72 hour.
Every part of 1g meter of bacterium Mixed culture: produce yellowish fiber monad 0.5g, bacillus amyloliquefaciens 0.5g and streptococcus fecalis 1g and inoculate Mixed culture in proportion.
The Mixed culture bacteria culture media is (g/L) by weight: glucose 5g, beef extract 6g, (NH
4)
2SO
45g and MgSO
4.7H
2O 0.5g.
The independent pure culture of bacillus coagulans, lactobacillus acidophilus and propionibacterium freudenreichii.
Every part of 1g meter of yeast Mixed culture: get S. cervisiae 0.1g, candida utili 0.5g and geotrichum candidum 0.5g and inoculate Mixed culture in proportion.Mixed culture Yeast Cultivation base is (g/L) by weight: glucose 15g, dusty yeast 5g, peptone 5g and (NH
4)
2SO
45g.
Every part of 1g of mould Mixed culture meter: get Trichoderma viride 0.5g and aspergillus oryzae 0.5g inoculates Mixed culture in proportion.The Mixed culture mould medium is (g/L) by weight: corn flour 15g, wheat bran 5g, powder of straw 5g and (NH
4)
2SO
43g.
Embodiment 9 (median dose)
The microorganism fungus kind of selecting for use by proportioning is: produce yellowish fiber monad, bacillus amyloliquefaciens, bacillus coagulans, lactobacillus acidophilus, propionibacterium freudenreichii, streptococcus fecalis, Trichoderma viride, aspergillus oryzae, S. cervisiae, candida utili and geotrichum candidum, the method for preparing cereals ferment essence feed microbe mother liquor is as follows:
Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary cultivation → fermentation tank mixes or single strain pure culture → bacterial classification makes up → vacuum packaging → finished product in proportion.
Slant strains is cultivated: above-mentioned bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3% cultivates 19-30h.
Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m
3/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-72 hour.
Every part of 1g meter of bacterium Mixed culture: produce yellowish fiber monad 0.75g, bacillus amyloliquefaciens 1g and streptococcus fecalis 2g and inoculate Mixed culture in proportion.
The Mixed culture bacteria culture media is (g/L) by weight: glucose 10g, beef extract 9g, (NH
4)
2SO
48g and MgSO
4.7H
2O 1.5g.
The independent pure culture of bacillus coagulans, lactobacillus acidophilus and propionibacterium freudenreichii.
Every part of 1g meter of yeast Mixed culture: get S. cervisiae 0.2g, candida utili 2g and geotrichum candidum 2g and inoculate Mixed culture in proportion.Mixed culture Yeast Cultivation base is (g/L) by weight: glucose 30g, dusty yeast 10g, peptone 10g and (NH
4)
2SO
410g.
Every part of 1g of mould Mixed culture meter: get Trichoderma viride 1g part and aspergillus oryzae 1g part is inoculated Mixed culture in proportion.The Mixed culture mould medium is (g/L) by weight: corn flour 22.5g, wheat bran 10g, powder of straw 10g and (NH
4)
2SO
46g.
Embodiment 10 (high dose)
The bran concentrated feed is formed by weight ratio: corn 470kg, dregs of beans 240kg, wheat bran 400kg, salt 30kg, stone flour 55kg, hay powder 90kg, powder of straw 80kg.
Embodiment 11 (low dosage)
The bran concentrated feed is formed by weight ratio: corn 270kg, dregs of beans 100kg, wheat bran 280kg, salt 10kg, stone flour 15kg, hay powder 30kg, powder of straw 20kg.
Embodiment 12 (medium metering)
The bran concentrated feed is formed by weight ratio: corn 370kg, dregs of beans 170kg, wheat bran 340kg, salt 20kg, stone flour 35kg, hay powder 60kg, powder of straw 50kg.
Embodiment 13 (high dose)
Bran ferment essence feed microbe mother liquor selects for use bacterial classification to form by every part of 1g weight proportion: bacillus amyloliquefaciens 1.5g, bacillus coagulans 1.5g, lactobacillus acidophilus 1.5g, propionibacterium freudenreichii 3.5g, streptococcus fecalis 3.5g, bafillus natto 1.5g, Trichoderma viride 1.5g, aspergillus oryzae 1.5g, S. cervisiae 0.3g, Fan Shi kluyveromyces 1.5g.
Embodiment 14 (low dosage)
Bran ferment essence feed microbe mother liquor selects for use bacterial classification to form by every part of 1g weight proportion: bacillus amyloliquefaciens 0.5g, bacillus coagulans 0.5g, lactobacillus acidophilus 0.5g, propionibacterium freudenreichii 1.5g, streptococcus fecalis 1.5g, bafillus natto 0.5g, Trichoderma viride 0.5g, aspergillus oryzae 0.5g, S. cervisiae 0.1g, Fan Shi kluyveromyces 0.5g.
Embodiment 15 (medium metering)
Bran ferment essence feed microbe mother liquor selects for use bacterial classification to form by every part of 1g weight proportion: bacillus amyloliquefaciens 1g, bacillus coagulans 1g, lactobacillus acidophilus 1g, propionibacterium freudenreichii 2.5g, streptococcus fecalis 2.5g, bafillus natto 1g, Trichoderma viride 1g, aspergillus oryzae 1g, S. cervisiae 0.2g, Fan Shi kluyveromyces 1g.
Embodiment 16 (high dose)
The microorganism fungus kind of selecting for use by proportioning is: bacillus amyloliquefaciens, bacillus coagulans, lactobacillus acidophilus, propionibacterium freudenreichii, streptococcus fecalis, bafillus natto, Trichoderma viride, aspergillus oryzae, S. cervisiae and Fan Shi kluyveromyces, the method for preparing bran ferment essence feed microbe mother liquor is as follows:
Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → bacterial classification makes up → vacuum packaging → finished product in proportion.
Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30h.
Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m
3/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-72 hour.
Every part of 1g meter of bacterium Mixed culture: bacillus amyloliquefaciens 1.5g, streptococcus fecalis 3.5g and bafillus natto 1.5g inoculate Mixed culture in proportion.
The Mixed culture bacteria culture media is (g/L) by weight: glucose 15g, beef extract 12g, (NH
4)
2SO
411g and MgSO
4.7H
2O 2.5g.
The independent pure culture of bacillus coagulans, lactobacillus acidophilus and propionibacterium freudenreichii.
Every part of 1g meter of yeast Mixed culture: S. cervisiae 0.3g and Fan Shi kluyveromyces 1.5g inoculate Mixed culture according to the above ratio.Mixed culture Yeast Cultivation base is (g/L) by weight: glucose 45g, dusty yeast 15g, peptone 15g and (NH
4)
2SO
415g.
Every part of 1g meter of mould Mixed culture: Trichoderma viride 1.5g and aspergillus oryzae 1.5g inoculate Mixed culture in proportion.The Mixed culture mould medium is (g/L) by weight: corn flour 30g, wheat bran 15g powder of straw 15g and (NH
4)
2SO
49g.
Embodiment 17 (low dosage)
The microorganism fungus kind of selecting for use by proportioning is: bacillus amyloliquefaciens, bacillus coagulans, lactobacillus acidophilus, propionibacterium freudenreichii, streptococcus fecalis, bafillus natto, Trichoderma viride, aspergillus oryzae, S. cervisiae and Fan Shi kluyveromyces, the method for preparing bran ferment essence feed microbe mother liquor is as follows:
Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → bacterial classification makes up → vacuum packaging → finished product in proportion.
Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30h.
Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m
3/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-72 hour.
Every part of 1g meter of bacterium Mixed culture: bacillus amyloliquefaciens 0.5g, streptococcus fecalis 1.5g and bafillus natto 0.5g inoculate Mixed culture in proportion.
The Mixed culture bacteria culture media is (g/L) by weight: glucose 5g, beef extract 6g, (NH
4)
2SO
45g and MgSO
4.7H
2O 0.5.
The independent pure culture of bacillus coagulans, lactobacillus acidophilus and propionibacterium freudenreichii.
Every part of 1g meter of yeast Mixed culture: S. cervisiae 0.1g and Fan Shi kluyveromyces 0.5g inoculate Mixed culture according to the above ratio.Mixed culture Yeast Cultivation base is (g/L) by weight: glucose 15g, dusty yeast 5g, peptone 5g and (NH
4)
2SO
45g.
Every part of 1g meter of mould Mixed culture: Trichoderma viride 0.5g and aspergillus oryzae 0.5g inoculate Mixed culture in proportion.The Mixed culture mould medium is (g/L) by weight: corn flour 15g, wheat bran 5g powder of straw 5g and (NH
4)
2SO
43g.
Embodiment 18 (median dose)
The microorganism fungus kind of selecting for use by proportioning is: bacillus amyloliquefaciens, bacillus coagulans, lactobacillus acidophilus, propionibacterium freudenreichii, streptococcus fecalis, bafillus natto, Trichoderma viride, aspergillus oryzae, S. cervisiae and Fan Shi kluyveromyces, the method for preparing bran ferment essence feed microbe mother liquor is as follows:
Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → bacterial classification makes up → vacuum packaging → finished product in proportion.
Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30h.
Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m
3/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-72 hour.
Every part of 1g meter of bacterium Mixed culture: bacillus amyloliquefaciens 1g, streptococcus fecalis 2.5g and bafillus natto 1g inoculate Mixed culture in proportion.
The Mixed culture bacteria culture media is (g/L) by weight: glucose 10g, beef extract 9g, (NH
4)
2SO
48g and MgSO
4.7H
2O 1.5g.
The independent pure culture of bacillus coagulans, lactobacillus acidophilus and propionibacterium freudenreichii.
Every part of 1g meter of yeast Mixed culture: S. cervisiae 0.2g and Fan Shi kluyveromyces 1g inoculate Mixed culture according to the above ratio.Mixed culture Yeast Cultivation base is (g/L) by weight: glucose 30g, dusty yeast 10g, peptone 10g and (NH
4)
2SO
410g.
Every part of 1g meter of mould Mixed culture: Trichoderma viride 1g and aspergillus oryzae 1g inoculate Mixed culture in proportion.The Mixed culture mould medium is (g/L) by weight: corn flour 22.5g, wheat bran 10g powder of straw 10g and (NH
4)
2SO
46g.
Embodiment 19 (high dose)
Dregs of beans class concentrated feed is formed by weight ratio: corn 350kg, dregs of beans 450kg, soybean 130kg, wheat bran 150kg, oil cake of flax seed 170kg, salt 30kg, stone flour 55kg, hay powder 90kg, powder of straw 80kg.
Embodiment 20 (low dosage)
Dregs of beans class concentrated feed is formed by weight ratio: corn 150kg, dregs of beans 250kg, soybean 30kg, wheat bran 80kg, oil cake of flax seed 70kg, salt 10kg, stone flour 15kg, hay powder 30kg, powder of straw 20kg.
Embodiment 21 (medium metering)
Dregs of beans class concentrated feed is formed by weight ratio: corn 250kg, dregs of beans 350kg, soybean 80kg, wheat bran 115kg, oil cake of flax seed 120kg, salt 20kg, stone flour 35kg, hay powder 60kg, powder of straw 50kg.
Embodiment 22 (high metering)
The bacterial classification that dregs of beans class ferment essence feed microbe mother liquor is selected for use is formed by every part of 1g weight proportion: bacillus amyloliquefaciens 1.5g, bacillus coagulans 1.5g, lactobacillus acidophilus 1.5g, propionibacterium freudenreichii 1.5g, streptococcus fecalis 1.5g, bafillus natto 1.5g, Trichoderma viride 2.5g, aspergillus oryzae 3.5g, geotrichum candidum 1.5g and Fan Shi kluyveromyces 1.5g.
Embodiment 23 (low dosage)
Dregs of beans class ferment essence feed microbe mother liquor selects for use bacterial classification to form by every part of 1g weight proportion: bacillus amyloliquefaciens 0.5g, bacillus coagulans 0.5g, lactobacillus acidophilus 0.5g, propionibacterium freudenreichii 0.5g, streptococcus fecalis 0.5g, bafillus natto 0.5g, Trichoderma viride 1.5g, aspergillus oryzae 2.5g, geotrichum candidum 0.5g and Fan Shi kluyveromyces 0.5g.
Embodiment 24 (medium metering)
Dregs of beans class ferment essence feed microbe mother liquor selects for use bacterial classification to form by every part of 1g weight proportion: bacillus amyloliquefaciens 1g, bacillus coagulans 1g, lactobacillus acidophilus 1g, propionibacterium freudenreichii 1g, streptococcus fecalis 1g, bafillus natto 1g, Trichoderma viride 2g, aspergillus oryzae 3g, geotrichum candidum 1g and Fan Shi kluyveromyces 1g.
Embodiment 25 (high dose)
The microorganism fungus kind of selecting for use by proportioning is: bacillus amyloliquefaciens, bacillus coagulans, lactobacillus acidophilus, propionibacterium freudenreichii, streptococcus fecalis, bafillus natto, Trichoderma viride, aspergillus oryzae, geotrichum candidum and Fan Shi kluyveromyces, and the method for preparing dregs of beans class ferment essence feed microbe mother liquor is as follows:
Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → bacterial classification makes up → vacuum packaging → finished product in proportion.
Slant strains is cultivated: above-mentioned bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30h.
Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m
3/ h, tank pressure 0.02-0.09 Mpa, inoculum concentration 1.5-7.5%, time 19-72 hour.
Every part of 1g meter of bacterium Mixed culture: bacillus amyloliquefaciens 1.5g, streptococcus fecalis 1.5g and bafillus natto 1.5g inoculate Mixed culture in proportion.
The Mixed culture bacteria culture media is (g/L) by weight: glucose 15g, beef extract 12g, (NH
4)
2SO
411g and MgSO
4.7H
2O 2.5g.
The independent pure culture of bacillus coagulans, lactobacillus acidophilus and propionibacterium freudenreichii.
Every part of 1g of yeast Mixed culture meter: get geotrichum candidum 1.5g and Fan Shi kluyveromyces 1.5g inoculates Mixed culture in proportion.Mixed culture Yeast Cultivation base is (g/L) by weight: glucose 45g, dusty yeast 15g, peptone 15g and (NH
4)
2SO
415g.
Every part of 1g of mould mould Mixed culture meter: get Trichoderma viride 3.5g and aspergillus oryzae 3.5g inoculates Mixed culture in proportion, Mixed culture mould medium (g/L): corn flour 30g, wheat bran 15g, powder of straw 15g, (NH
4)
2SO
49g.
Embodiment 26 (low dosage)
The microorganism fungus kind of selecting for use by proportioning is: bacillus amyloliquefaciens, bacillus coagulans, lactobacillus acidophilus, propionibacterium freudenreichii, streptococcus fecalis, bafillus natto, Trichoderma viride, aspergillus oryzae, geotrichum candidum and Fan Shi kluyveromyces, and the method for preparing dregs of beans class ferment essence feed microbe mother liquor is as follows:
Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → bacterial classification makes up → vacuum packaging → finished product in proportion.
Slant strains is cultivated: above-mentioned bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30h.
Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m
3/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-72 hour.
Every part of 1g meter of bacterium Mixed culture: bacillus amyloliquefaciens 0.5g, streptococcus fecalis 0.5g and bafillus natto 0.5g inoculate Mixed culture in proportion.
The Mixed culture bacteria culture media is (g/L) by weight: glucose 5g, beef extract 6g, (NH
4)
2SO
45g and MgSO
4.7H
2O 0.5g.
The independent pure culture of bacillus coagulans, lactobacillus acidophilus and propionibacterium freudenreichii.
Every part of 1g of yeast Mixed culture meter: get geotrichum candidum 0.5g and Fan Shi kluyveromyces 0.5g inoculates Mixed culture in proportion.Mixed culture Yeast Cultivation base is (g/L) by weight: glucose 15g, dusty yeast 5g, peptone 5g and (NH
4)
2SO
45g.
Every part of 1g of mould mould Mixed culture meter: get Trichoderma viride 1.5g and aspergillus oryzae 1.5g inoculates Mixed culture in proportion, Mixed culture mould medium (g/L): corn flour 15g, wheat bran 5g, powder of straw 5g, (NH
4)
2SO
43g.
Embodiment 27 (median dose)
The microorganism fungus kind of selecting for use by proportioning is: bacillus amyloliquefaciens, bacillus coagulans, lactobacillus acidophilus, propionibacterium freudenreichii, streptococcus fecalis, bafillus natto, Trichoderma viride, aspergillus oryzae, geotrichum candidum and Fan Shi kluyveromyces, and the method for preparing dregs of beans class ferment essence feed microbe mother liquor is as follows:
Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → bacterial classification makes up → vacuum packaging → finished product in proportion.
Slant strains is cultivated: above-mentioned bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30h.
Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m
3/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-72 hour.
Every part of 1g meter of bacterium Mixed culture: bacillus amyloliquefaciens 1g, streptococcus fecalis 1g and bafillus natto 1g inoculate Mixed culture in proportion.
The Mixed culture bacteria culture media is (g/L) by weight: glucose 10g, beef extract 9g, (NH
4)
2SO
48g and MgSO
4.7H
2O 1.5g.
The independent pure culture of bacillus coagulans, lactobacillus acidophilus and propionibacterium freudenreichii.
Every part of 1g of yeast Mixed culture meter: get geotrichum candidum 1g and Fan Shi kluyveromyces 1g inoculates Mixed culture in proportion.Mixed culture Yeast Cultivation base is (g/L) by weight: glucose 30g, dusty yeast 10g, peptone 10g and (NH
4)
2SO
410g.
Every part of 1g of mould mould Mixed culture meter: get Trichoderma viride 2.5g and aspergillus oryzae 2.5g inoculates Mixed culture in proportion, Mixed culture mould medium (g/L): corn flour 22.5g, wheat bran 10g, powder of straw 10g, (NH
4)
2SO
46g.
Embodiment 28
Select cereals ferment essence feed microbe mother liquor for use by proportioning, the method for preparing cereals ferment essence feed is as follows:
Add cereals ferment essence feed microbe mother liquor in cereals ferment essence feed, process stirs, vacuum packaging, and apparent temperature fermentation 7-30 days obtains finished feed at last.
Embodiment 29
Select bran ferment essence feed microbe mother liquor for use by proportioning, the method for preparing bran ferment essence feed is as follows:
Add bran ferment essence feed microbe mother liquor in bran ferment essence feed, process stirs, vacuum packaging, and apparent temperature fermentation 7-30 days obtains finished feed at last.
Embodiment 30
Select dregs of beans class ferment essence feed microbe mother liquor for use by proportioning, the method for preparing dregs of beans class ferment essence feed is as follows:
Add dregs of beans class ferment essence feed microbe mother liquor in dregs of beans class ferment essence feed, process stirs, vacuum packaging, and apparent temperature fermentation 7-30 days obtains finished feed at last.
Claims (10)
1, one kind of multiple microbial fermentation concentrated feeds is characterized in that the cereals concentrated feed comprises that following component forms by weight proportion per ton: corn 44-64%, dregs of beans 11-25%, wheat bran 11-23%, salt 1-3%, stone flour 1.5-5.5%, hay powder 3-9%, powder of straw 2-8%.
2, a kind of ferment essence feed microbe mother liquor is characterized in that cereals ferment essence feed microbe mother liquor selects for use bacterial classification to comprise that following component forms by cereals ferment essence feed weight proportion per ton: produce yellowish fiber monad 0.5-1 part, bacillus amyloliquefaciens 0.5-1.5 part, bacillus coagulans 0.5-1.5 part, lactobacillus acidophilus 0.5-1.5 part, propionibacterium freudenreichii 1.5-3.5 part, streptococcus fecalis 1-3 part, Trichoderma viride 0.5-1.5 part, aspergillus oryzae 0.5-1.5 part, S. cervisiae 0.1-0.3 part, candida utili 0.5-1.5 part and geotrichum candidum 0.5-1.5 part.
3, a kind of preparation method of ferment essence feed microbe mother liquor, it is characterized in that the microorganism fungus kind of selecting for use by proportioning is: produce yellowish fiber monad, bacillus amyloliquefaciens, bacillus coagulans, lactobacillus acidophilus, propionibacterium freudenreichii, streptococcus fecalis, Trichoderma viride, aspergillus oryzae, S. cervisiae, candida utili and geotrichum candidum, the method for preparing cereals ferment essence feed microbe mother liquor is as follows:
Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary cultivation → fermentation tank mixes or single strain pure culture → bacterial classification makes up → vacuum packaging → finished product in proportion;
Slant strains is cultivated: above-mentioned bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves; Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3% cultivates 19-30h;
Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m
3/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-72 hour;
Bacterium Mixed culture: produce yellowish fiber monad 0.5-1 part, bacillus amyloliquefaciens 0.5-1.5 part and streptococcus fecalis 1-3 part and inoculate Mixed culture in proportion;
The Mixed culture bacteria culture media is (g/L) by weight: glucose 5g-15g, beef extract 6g-12g, (NH
4)
2SO
45g-11g and MgSO
47H
2O0.5-2.5g;
The independent pure culture of bacillus coagulans, lactobacillus acidophilus and propionibacterium freudenreichii;
Yeast Mixed culture: get S. cervisiae 0.1-0.3 part, candida utili 0.5-1.5 part and geotrichum candidum 0.5-1.5 part and inoculate Mixed culture in proportion; The culture medium of yeast Mixed culture is (g/L) by weight: glucose 15g-45g, dusty yeast 5g-15g, peptone 5g-15g and (NH
4)
2SO
45g-15g;
Mould Mixed culture: get Trichoderma viride 0.5-1.5 part and aspergillus oryzae 0.5-1.5 part is inoculated Mixed culture in proportion; The Mixed culture mould medium is (g/L) by weight: corn flour 15g-30g, wheat bran 5g-15g, powder of straw 5g-15g and (NH
4)
2SO
43g-9g.
4, one kind of multiple microbial fermentation concentrated feeds is characterized in that the bran concentrated feed comprises that following component forms by weight proportion per ton: corn 27-47%, dregs of beans 10-24%, wheat bran 28-40%, salt 1-3%, stone flour 1.5-5.5%, hay powder 3-9%, powder of straw 2-8%.
5, a kind of ferment essence feed microbe mother liquor, it is characterized in that bran ferment essence feed microbe mother liquor selects for use bacterial classification to comprise that following component forms by bran ferment essence feed weight proportion per ton: bacillus amyloliquefaciens 0.5-1.5 part, bacillus coagulans 0.5-1.5 part, lactobacillus acidophilus 0.5-1.5 part, propionibacterium freudenreichii 1.5-3.5 part, streptococcus fecalis 1.5-3.5 part, bafillus natto 0.5-1.5 part, Trichoderma viride 0.5-1.5 part, aspergillus oryzae 0.5-1.5 part, S. cervisiae 0.1-0.3 part, Fan Shi kluyveromyces 0.5-1.5 part.
6, a kind of preparation method of ferment essence feed microbe mother liquor, it is characterized in that the microorganism fungus kind of selecting for use by proportioning is: bacillus amyloliquefaciens, bacillus coagulans, lactobacillus acidophilus, propionibacterium freudenreichii, streptococcus fecalis, bafillus natto, Trichoderma viride, aspergillus oryzae, S. cervisiae and Fan Shi kluyveromyces, the method for preparing bran ferment essence feed microbe mother liquor is as follows:
Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → bacterial classification makes up → vacuum packaging → finished product in proportion;
Slant strains is cultivated: above-mentioned bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves; Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30h;
Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m
3/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-72 hour;
The bacterium Mixed culture: bacillus amyloliquefaciens 0.5-1.5 part, streptococcus fecalis 1.5-3.5 part and bafillus natto 0.5-1.5 part are inoculated Mixed culture in proportion;
The Mixed culture bacteria culture media is (g/L) by weight: glucose 5g-15g, beef extract 6g-12g, (NH
4)
2SO
45g-11g and MgSO
47H
2O0.5-2.5g;
The independent pure culture of bacillus coagulans, lactobacillus acidophilus and propionibacterium freudenreichii;
The yeast Mixed culture: S. cervisiae 0.1-0.3 part and Fan Shi kluyveromyces 0.5-1.5 part are inoculated Mixed culture according to the above ratio; Mixed culture Yeast Cultivation base is (g/L) by weight: glucose 15g-45g, dusty yeast 5g-15g, peptone 5g-15g and (NH
4)
2SO
45g-15g;
The mould Mixed culture: Trichoderma viride 0.5-1.5 part and aspergillus oryzae 0.5-1.5 part are inoculated Mixed culture in proportion; The Mixed culture mould medium is (g/L) by weight: corn flour 15g-30g, wheat bran 5g-15g, powder of straw 5g-15g and (NH
4)
2SO
43g-9g.
7, one kind of multiple microbial fermentation concentrated feeds is characterized in that dregs of beans class concentrated feed comprises that following component forms by weight proportion per ton: corn 15-35%, dregs of beans 25-45%, soybean 3-13%, wheat bran 8-15%, oil cake of flax seed 7-17%, salt 1-3%, stone flour 1.5-5.5%, hay powder 3-9%, powder of straw 2-8%.
8, a kind of ferment essence feed microbe mother liquor, it is characterized in that dregs of beans class ferment essence feed microbe mother liquor selects for use bacterial classification to comprise that following component forms by dregs of beans class ferment essence feed weight proportion per ton: bacillus amyloliquefaciens 0.5-1.5 part, bacillus coagulans 0.5-1.5 part, lactobacillus acidophilus 0.5-1.5 part, propionibacterium freudenreichii 0.5-1.5 part, streptococcus fecalis 0.5-1.5 part, bafillus natto 0.5-1.5 part, Trichoderma viride 1.5-2.5 part, aspergillus oryzae 2.5-3.5 part, geotrichum candidum 0.5-1.5 part, Fan Shi kluyveromyces 0.5-1.5 part.
9, a kind of preparation method of ferment essence feed microbe mother liquor, it is characterized in that the microorganism fungus kind of selecting for use by proportioning is: bacillus amyloliquefaciens, bacillus coagulans, lactobacillus acidophilus, propionibacterium freudenreichii, streptococcus fecalis, bafillus natto, Trichoderma viride, aspergillus oryzae, geotrichum candidum and Fan Shi kluyveromyces, the method for preparing dregs of beans class ferment essence feed microbe mother liquor is as follows:
Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → bacterial classification makes up → vacuum packaging → finished product in proportion;
Slant strains is cultivated: above-mentioned bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves; Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30h;
Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m
3/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-72 hour;
The bacterium Mixed culture: bacillus amyloliquefaciens 0.5-1.5 part, streptococcus fecalis 0.5-1.5 part and bafillus natto 0.5-1.5 part are inoculated Mixed culture in proportion;
The Mixed culture bacteria culture media is (g/L) by weight: glucose 5g-15g, beef extract 6g-12g, (NH
4)
2SO
45g-11g and MgSO
47H
2O0.5-2.5g;
The independent pure culture of bacillus coagulans, lactobacillus acidophilus and propionibacterium freudenreichii;
Yeast Mixed culture: get geotrichum candidum 0.5-1.5 part and Fan Shi kluyveromyces 0.5-1.5 part is inoculated Mixed culture in proportion; Mixed culture Yeast Cultivation base is (g/L) by weight: glucose 15g-45g, dusty yeast 5g-15g, peptone 5g-15g and (NH
4)
2SO
45g-15g;
Mould Mixed culture: get Trichoderma viride 1.5-3.5 part and aspergillus oryzae 1.5-3.5 part is inoculated Mixed culture in proportion; The Mixed culture mould medium is (g/L) by weight: corn flour 15g-30g, wheat bran 5g-15g, powder of straw 5g-15g and (NH
4)
2SO
43g-9g.
10, realize the preparation method of claim 1 or 4 or 7 described multiple microbial fermentation concentrated feeds, it is characterized in that selecting cereals ferment essence feed microbe mother liquor or bran ferment essence feed microbe mother liquor or dregs of beans class ferment essence feed microbe mother liquor for use by proportioning, the method for preparing cereals ferment essence feed or bran ferment essence feed or dregs of beans class ferment essence feed is as follows:
Add corresponding microorganism mother liquor in cereals ferment essence feed or bran ferment essence feed or dregs of beans class ferment essence feed, process stirs, vacuum packaging, and apparent temperature fermentation 7-30 days obtains finished feed at last.
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