CN1295983C - Preparation method of enzyme enriched active yeast feed - Google Patents
Preparation method of enzyme enriched active yeast feed Download PDFInfo
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- CN1295983C CN1295983C CNB2005100493372A CN200510049337A CN1295983C CN 1295983 C CN1295983 C CN 1295983C CN B2005100493372 A CNB2005100493372 A CN B2005100493372A CN 200510049337 A CN200510049337 A CN 200510049337A CN 1295983 C CN1295983 C CN 1295983C
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Abstract
The present invention discloses a method for preparing enzyme-enriched active yeast feed. Rhizopus oryzae is prepared into rhizopus oryzae mother culture, and geotrichum candidum is prepared into geotrichum candidum mother culture; candida utilis, arborescent candida and saccharomyces cerevisiae are prepared into yeast seed liquid, and the yeast seed liquid is respectively inoculated in solid-state fermentation culture media pretreated by means of alpha-amylase, saccharifying enzyme and cellulase; according to dry substances of the solid-state fermentation culture media, inoculation quantities comprise 0.5 to 2% of rhizopus oryzae, 12% of yeast seed liquid and 0.4 to 0.8% of geotrichum candidum mother culture; then, the yeast seed liquid is cultivated in a fermentation mode at the temperature of 33 to 35 DEG C for 28 to 30 hours and is dried in the sun or at the temperature of 38 to 45 DEG C. The enzyme-enriched active yeast feed prepared by means of the present invention is protein feed. Moreover, the total number of live yeast cells is more than or equal to 6*10<8> per gram, the vigor of saccharifying enzyme is more than or equal to 200U per gram, and the vigor of protease is more than or equal to 200U per gram. The yeast feed has the fragrance of wine, so animals like to eat the yeast feed. 0.1 to 5% of compound feed is added to the yeast feed, so the fertility performance of animals can be obviously improved.
Description
Technical field
The present invention relates to a kind of preparation method of enzyme enriched active yeast feed.
Background technology
The production of fodder yeast has had the history of decades in China, with its abundant B family vitamin, form, be rich in active digestion and UGF and in feedstuff industry, occupy, more with its lower price and can the part substituted fish meal and be subjected to production of fodder producer and numerous raisers' favor than consequence than reasonable amino acid.
In the animal daily ration, add yeast, increase along with the saccharomycete intake, saccharomycetic absolute magnitude and relative quantity increase, can promote breeding of profitable strains such as enteron aisle lactic acid bacteria, cellulose-decomposing bacteria and vigor improve, promote stomach and intestine to the decomposition of feed nutrients, synthetic, digestion, absorb and utilize, increase feed intake.Simultaneously, saccharomycete can effectively suppress the breeding of pathogenic microorganism, participate in the competition of pathogenic microorganism flora survivability, repel pathogen adhering on the gastrointestinal mucosa surface, assist body to eliminate toxin and metabolite thereof, prevent the absorption of toxin and refuse, strengthen immunity of organism and resistance against diseases, stimulate the cub stomach and intestine to grow, control domestic animal disease of digestive system is played the effect of probiotic.
Yet present fodder yeast product also exists many deficiencies: few as viable yeast bacterium quantity, the metabolic activity of yeast cells in alimentary canal is very low; Deficiency such as low, palatability difference of enzymatic activity in the fodder yeast.
For bringing into play the effect of yeast feed better, the present invention starts with from the measure of plurality of enzymes abilities such as saccharomycetic screening and raising product carbohydrase, protease, study many bacterial classifications co-incubation condition, the complex enzyme forage yeast that works out is not only a kind of new protein feed resources, have wine flavour improves palatability, a kind of especially active material that has the regulation and control intestinal microflora and improve feed digestibility.
Summary of the invention
The preparation method who the purpose of this invention is to provide a kind of enzyme enriched active yeast feed.
It makes tanekoji of Rhizopus oryzae with Rhizopus oryzae, geotrichum candidum is made geotrichum candidum kind song, candida utili, Candida arborea and saccharomyces cerevisiae are made yeast starter liquid, be inoculated in respectively through AMS, carbohydrase and cellulase and carry out in the pretreated solid-state fermentation culture medium, by the solid-state fermentation culture medium dry, inoculum concentration is a tanekoji of Rhizopus oryzae 0.5～2%, yeast starter liquid 12%, geotrichum candidum kind song 0.4～0.8%, at 33-35 ℃ of fermented and cultured 28-30 hour, through drying or 38～45 ℃ of dryings.Wherein in the yeast starter liquid, candida utili seed liquor, Candida arborea seed liquor, saccharomyces cerevisiae seed liquor ratio are 20-10: 20-10: 1.
Solid-state fermentation culture medium is: wheat bran 40-60%, flour 3-10%, vinasse 15-20%, bean cake powder 20-25%, the mixture that urea 2-5% forms.
Carrying out preprocess method with solid-state fermentation culture medium through AMS, carbohydrase and cellulase is: with solid-state fermentation culture medium and water with 1: the 0.6-0.7 ratio mixes, sterilization in boiling 30-40 minute under 0.1-0.15Mpa pressure, in solid-state fermentation culture medium, add again, carried out preliminary treatment 1-2 hour by solid-state fermentation culture medium dry 0.01%-0.02% AMS, 0.005%-0.01% carbohydrase and 0.005%-0.01% cellulase.
The enzyme enriched active yeast feed that the present invention prepares is not only a kind of microprotein feed, and active yeast cell sum 〉=6 * 10
8Individual/gram, saccharifying enzymic activity 〉=200U/g, prolease activity 〉=200U/g has wine flavour, and animal happiness food is used for livestock and poultry, aquaculture, and the addition of amounting to mixed feed is 0.1～5%, can obviously improve breeding performonce fo animals.
The specific embodiment
With wheat bran, flour, vinasse, dregs of beans and urea is raw material, through adding water, boiling sterilization, inoculate Rhizopus oryzae (Rhizopus oryzae AS 3.866), geotrichum candidum (Geotrichum candidum AS 2.361), candida utili (Candida utilis ACCC2102), Candida arborea (Candida arborea AS 2.566), saccharomyces cerevisiae microorganism probios such as (Saccharomycescerevisiae ACCC 2042) after adding AMS, carbohydrase and cellulase preliminary treatment, diastatic fermentation is cultivated into complex enzyme high-activity yeast feed.
1. plant bent and the seed liquor preparation
Tanekoji of Rhizopus oryzae is cultivated
Rhizopus oryzae (Rhizopus oryzae AS 3.866): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences.Enlarge and cultivate flow process: wheat bran test tube (original seed) → triangular flask seed → bent box kind song (wheat bran) → tanekoji of Rhizopus oryzae
Wheat bran adds water 70-75%, 0.1-0.15Mpa boiling is 30 minutes under the condition, is cooled to the Rhizopus oryzae that 35 ℃ of inoculations are cultivated through triangular flask, inoculum concentration 0.3%, be incubated 30 ℃ of cultivations, heat up through the germination of 12h bacterial classification, go up bent box and cultivate, total incubation time 30-36h, can go out the room drying, bake out temperature does not surpass 38 ℃ early stage, and the later stage does not surpass 45 ℃, and moisture 10% is the Rhizopus oryzae sort of quyi.
The geotrichum candidum kind is bent cultivates
Geotrichum candidum (Geotrichum candidum AS 2.361): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences.Enlarge and cultivate flow process: slant tube (original seed) → slant tube → triangular flask seed (brewer's wort) → kind of song
Wheat bran adds water 60-70%, and boiling is 30 minutes under the boiling 0.1-0.15Mpa condition, is cooled to 35 ℃ and connects triangular flask geotrichum candidum seed, and inoculum concentration 0.5% is incubated 33-35 ℃ of cultivation, and total incubation time 24h can go out the room and dry to moisture 10% and be geotrichum candidum kind song.
Yeast starter liquid is cultivated
Candida utili (Candida utilis ACCC 2102): available from Chinese agriculture microorganism fungus kind preservation administrative center; Candida arborea (Candida arborea AS 2.566): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences; Saccharomyces cerevisiae (Saccharomyces cerevisiae ACCC 2042): available from Chinese agriculture microorganism fungus kind preservation administrative center.
Enlarge and cultivate flow process (three bacterial classification flow process unanimities, cultivate separately): former bacterium test tube → little triangular flask seed (liquid glucose 100ml, pol 7-8 ° of Be) 30 ℃ of heat insulating culture 10-12h → change over to big triangular flask (liquid glucose 600ml, pol 7-8 ° of Be), 30 ℃ of heat insulating culture 10-12h → change over to (syrup 12-13kg in the Ka Shi jar, pol 7-8 ° of Be expansion cultivation) 10～12h → Ka Shi jar yeast starter is spread cultivation at the distiller's yeast cylinder, and (culture medium is that every cylinder is put clear water 200-300kg in 30 ℃ of cultivations, flour 30kg, wheat bran 10kg) total incubation time 18-20h is yeast starter liquid.
2. solid-state fermentation culture medium preliminary treatment
With wheat bran 40-60%, flour 3-10%, vinasse 15-20%, bean cake powder 20-25%, urea 2-5% composition solid-state fermentation culture medium and water are with 1: the 0.6-0.7 ratio mixes, under 0.1-0.15Mpa pressure, sterilized in boiling 30-40 minute, close inlet valve after the sterilization, shelving 30-40 minute discharging, with blower fan blast-cold material to 40～45 ℃, transfer PH5.5～6, add in solid-state fermentation culture medium again that (enzyme work is in 5000U/ μ g enzyme by solid-state fermentation culture medium dry 0.01%-0.02% AMS, the enzyme activity determination method is with reference to GB8275-87), (enzyme work is in 50000U/g for the 0.005%-0.01% carbohydrase, the enzyme activity determination method is with reference to GB8276-87) and the 0.005%-0.01% cellulase (enzyme work is in 20000U/g, the enzyme activity determination method is with reference to NY/T 912-2004 GB 8276-87), beat evenly with raising the bran machine, carried out preliminary treatment 1-2 hour.
3. inoculation
Through pretreated solid-state fermentation culture medium, the ventilation blast-cold is to 33-35 ℃, inoculate (by the culture medium dry) tanekoji of Rhizopus oryzae 0.5～2% respectively, yeast starter liquid (candida utili seed liquor, Candida arborea seed liquor, saccharomyces cerevisiae seed liquor 20-10: 20-10: 1) 12%, geotrichum candidum kind song 0.4～0.8% is raised bran machine secondary and is beaten and mix evenly, floating charge level, heat insulating culture, fermentation temperature are controlled at about 30 ℃, voluntarily fermented and cultured.
4. fermentation management
Fermentation temperature has mainly been managed in fermentation.Open temperature-controlled instrument, connect fan power, the temperature upper control limit is 35 ℃, is limited to 33 ℃ down.The diligent observation, diligent test.Temperature controller probe drop point wants correct, the degree of depth will reach 10cm (doing contrast but plug in thermometer on the limit), mainly prevent probe, temperature controller and connection instrument fault, begin after general 6 hours to heat up, wind is touring in the early stage used chamber, and is higher or expect that the temperature rise temperature is too quickly when weather temperature, but air door outside the opening chamber reaches the purpose of regulating growth temperature.Fermentation in general 28-30 hour finishes, and can strengthen air door, opens door, does not make the material temperature surpass 38 ℃, waits for out the room drying.
5. dry
Material can go out the room drying after cultivating maturation, and when weather is sunny, dry ground on the scene as far as possible, and one can save the energy, and two make microorganism keep more vigor.
During it rain, can only dry, expect temperature control early stage in 38 ℃, and later stage material temperature control is in 45 ℃, and moisture can go out the room less than 10%.
Be semi-finished product after the material drying, experience physical and chemical index and microbiological indicator detect, and reach yeast cells sum 〉=8 * 10
8Individual/gram, active yeast cell sum 〉=6 * 10
8Individual/gram, saccharifying enzymic activity 〉=200U/g, prolease activity 〉=200U/g is enzyme enriched active yeast feed.
The color and luster of enzyme enriched active yeast feed is faint yellow to pale brown look, has wine flavour.Enzyme enriched active yeast can be applicable to mixed feed, concentrated feed, premix or additive, and the content that its addition is amounted in the mixed feed is: 0.1～5%.
Embodiment 1:
1. plant bent and the seed liquor preparation
Tanekoji of Rhizopus oryzae is cultivated Rhizopus oryzae (Rhizopus oryzae AS 3.866): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences.Enlarge and cultivate flow process: wheat bran test tube (original seed) → triangular flask seed → bent box kind song (wheat bran) → tanekoji of Rhizopus oryzae
Wheat bran adds water 70%, 0.1Mpa boiling is 30 minutes under the condition, is cooled to the Rhizopus oryzae that 35 ℃ of inoculations are cultivated through triangular flask, inoculum concentration 0.3%, be incubated 30 ℃ of cultivations, heat up through the germination of 12h bacterial classification, go up bent box and cultivate, total incubation time 30h, can go out the room drying, bake out temperature does not surpass 38 ℃ early stage, and the later stage does not surpass 45 ℃, and moisture 10% is the Rhizopus oryzae sort of quyi.
The geotrichum candidum kind is bent cultivates
Geotrichum candidum (Geotrichum candidum AS 2.361): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences.Enlarge and cultivate flow process: slant tube (original seed) → slant tube → triangular flask seed (brewer's wort) → kind of song
Wheat bran adds water 60%, and boiling is 30 minutes under the boiling 0.1Mpa condition, is cooled to 35 ℃ and connects triangular flask geotrichum candidum seed, and inoculum concentration 0.5% is incubated 33 ℃ of cultivations, and total incubation time 24h can go out the room and dry to moisture 10% and be geotrichum candidum kind song.
Yeast starter liquid is cultivated
Candida utili (Candida utilis ACCC 2102): available from Chinese agriculture microorganism fungus kind preservation administrative center; Candida arborea (Candida arborea AS 2.566): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences; Saccharomyces cerevisiae (Saccharomyces cerevisiae ACCC 2042): available from Chinese agriculture microorganism fungus kind preservation administrative center.
Enlarge and cultivate flow process (three bacterial classification flow process unanimities, cultivate separately): former bacterium test tube → little triangular flask seed (liquid glucose 100ml, 7 ° of Be of pol) 30 ℃ of heat insulating culture 10h → change over to big triangular flask (liquid glucose 600ml, 7 ° of Be of pol), 30 ℃ of heat insulating culture 10h → change over to (syrup 12kg in the Ka Shi jar, 7 ° of Be expansion of pol cultivation) 10h → Ka Shi jar yeast starter is spread cultivation at the distiller's yeast cylinder, and (culture medium is that every cylinder is put clear water 200kg in 30 ℃ of cultivations, flour 30kg, wheat bran 10kg) total incubation time 18h is yeast starter liquid.
2. solid-state fermentation culture medium preliminary treatment
With wheat bran 40%, flour 10%, vinasse 20%, bean cake powder 25%, solid-state fermentation culture medium formed by urea 5% and water mixes with 1: 0.6 ratio, boiling was sterilized in 30 minutes under 0.1Mpa pressure, close inlet valve after the sterilization, shelving discharging in 30 minutes, with blower fan blast-cold material to 40 ℃, transfer PH5.5, in solid-state fermentation culture medium, add again by solid-state fermentation culture medium dry 0.01% AMS (enzyme work is in 5000U/ μ g enzyme, and the enzyme activity determination method is with reference to GB 8275-87), (enzyme work is in 50000U/g for 0.005% carbohydrase, the enzyme activity determination method is with reference to GB 8276-87) and 0.005% cellulase (enzyme work is in 20000U/g, the enzyme activity determination method is with reference to NY/T 912-2004 GB 8276-87), beat evenly with raising the bran machine, carried out preliminary treatment 1 hour.
3. inoculation
Through pretreated solid-state fermentation culture medium, ventilation blast-cold to 33 ℃, inoculate (by the culture medium dry) tanekoji of Rhizopus oryzae 0.5% respectively, yeast starter liquid (candida utili seed liquor, Candida arborea seed liquor, saccharomyces cerevisiae seed liquor 10: 10: 1) 12%, geotrichum candidum kind song 0.4% is raised bran machine secondary and is beaten and mix evenly, floating charge level, heat insulating culture, fermentation temperature are controlled at about 30 ℃, voluntarily fermented and cultured.
4. fermentation management
Fermentation temperature has mainly been managed in fermentation.Open temperature-controlled instrument, connect fan power, the temperature upper control limit is 35 ℃, is limited to 33 ℃ down.The diligent observation, diligent test.Temperature controller probe drop point wants correct, the degree of depth will reach 10cm (doing contrast but plug in thermometer on the limit), mainly prevent probe, temperature controller and connection instrument fault, begin after general 6 hours to heat up, wind is touring in the early stage used chamber, and is higher or expect that the temperature rise temperature is too quickly when weather temperature, but air door outside the opening chamber reaches the purpose of regulating growth temperature.Finish through fermentation in 28 hours, can strengthen air door, open door, do not make the material temperature surpass 38 ℃, wait for out the room drying.
5. dry
Material can go out the room drying after cultivating maturation, and when weather is sunny, dry ground on the scene as far as possible, and one can save the energy, and two make microorganism keep more vigor.
During it rain, can only dry, expect temperature control early stage in 38 ℃, and later stage material temperature control is in 45 ℃, and moisture can go out the room less than 10%.
Be semi-finished product after the material drying, experience physical and chemical index and microbiological indicator detect, and reach yeast cells sum 〉=8 * 10
8Individual/gram, active yeast cell sum 〉=6 * 10
8Individual/gram, saccharifying enzymic activity 〉=200U/g, prolease activity 〉=200U/g is enzyme enriched active yeast feed.
Embodiment 2:
1. plant bent and the seed liquor preparation
Tanekoji of Rhizopus oryzae is cultivated
Rhizopus oryzae (Rhizopus oryzae AS 3.866): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences.Enlarge and cultivate flow process: wheat bran test tube (original seed) → triangular flask seed → bent box kind song (wheat bran) → tanekoji of Rhizopus oryzae
Wheat bran adds water 75%, 0.15Mpa boiling is 30 minutes under the condition, is cooled to the Rhizopus oryzae that 35 ℃ of inoculations are cultivated through triangular flask, inoculum concentration 0.3%, be incubated 30 ℃ of cultivations, heat up through the germination of 12h bacterial classification, go up bent box and cultivate, total incubation time 36h, can go out the room drying, bake out temperature does not surpass 38 ℃ early stage, and the later stage does not surpass 45 ℃, and moisture 10% is the Rhizopus oryzae sort of quyi.
The geotrichum candidum kind is bent cultivates
Geotrichum candidum (Geotrichum candidum AS 2.361): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences.Enlarge and cultivate flow process: slant tube (original seed) → slant tube → triangular flask seed (brewer's wort) → kind of song
Wheat bran adds water 70%, and boiling is 30 minutes under the boiling 0.15Mpa condition, is cooled to 35 ℃ and connects triangular flask geotrichum candidum seed, and inoculum concentration 0.5% is incubated 35 ℃ of cultivations, and total incubation time 24h can go out the room and dry to moisture 10% and be geotrichum candidum kind song.
Yeast starter liquid is cultivated
Candida utili (Candida utilis ACCC 2102): available from Chinese agriculture microorganism fungus kind preservation administrative center; Candida arborea (Candida arborea AS 2.566): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences; Saccharomyces cerevisiae (Saccharomyces cerevisiae ACCC 2042): available from Chinese agriculture microorganism fungus kind preservation administrative center.
Enlarge and cultivate flow process (three bacterial classification flow process unanimities, cultivate separately): former bacterium test tube → little triangular flask seed (liquid glucose 100ml, 8 ° of Be of pol) 30 ℃ of heat insulating culture 12h → change over to big triangular flask (liquid glucose 600ml, 8 ° of Be of pol), 30 ℃ of heat insulating culture 12h → change over to (syrup 12-13kg in the Ka Shi jar, 8 ° of Be expansion of pol cultivation) 12h → Ka Shi jar yeast starter is spread cultivation at the distiller's yeast cylinder, and (culture medium is that every cylinder is put clear water 300kg in 30 ℃ of cultivations, flour 30kg, wheat bran 10kg) total incubation time 20h is yeast starter liquid.
2. solid-state fermentation culture medium preliminary treatment
With wheat bran 60%, flour 3%, vinasse 15%, bean cake powder 20%, solid-state fermentation culture medium formed by urea 2% and water mixes with 1: 0.7 ratio, boiling was sterilized in 40 minutes under 0.15Mpa pressure, close inlet valve after the sterilization, shelving discharging in 40 minutes, with blower fan blast-cold material to 45 ℃, transfer PH to 6, in solid-state fermentation culture medium, add again by solid-state fermentation culture medium dry 0.02% AMS (enzyme work is in 5000U/ μ g enzyme, and the enzyme activity determination method is with reference to GB 8275-87), (enzyme work is in 50000U/g for 0.01% carbohydrase, the enzyme activity determination method is with reference to GB 8276-87) and 0.01% cellulase (enzyme work is in 20000U/g, the enzyme activity determination method is with reference to NY/T 912-2004 GB 8276-87), beat evenly with raising the bran machine, carried out preliminary treatment 2 hours.
3. inoculation
Through pretreated solid-state fermentation culture medium, ventilation blast-cold to 35 ℃, inoculate (by the culture medium dry) tanekoji of Rhizopus oryzae 2% respectively, yeast starter liquid (candida utili seed liquor, Candida arborea seed liquor, saccharomyces cerevisiae seed liquor 20: 20: 1) 12%, geotrichum candidum kind song 0.8% is raised bran machine secondary and is beaten and mix evenly, floating charge level, heat insulating culture, fermentation temperature are controlled at about 30 ℃, voluntarily fermented and cultured.
4. fermentation management
Fermentation temperature has mainly been managed in fermentation.Open temperature-controlled instrument, connect fan power, the temperature upper control limit is 35 ℃, is limited to 33 ℃ down.The diligent observation, diligent test.Temperature controller probe drop point wants correct, the degree of depth will reach 10cm (doing contrast but plug in thermometer on the limit), mainly prevent probe, temperature controller and connection instrument fault, begin after general 6 hours to heat up, wind is touring in the early stage used chamber, and is higher or expect that the temperature rise temperature is too quickly when weather temperature, but air door outside the opening chamber reaches the purpose of regulating growth temperature.Fermentation in general 30 hours finishes, and can strengthen air door, opens door, does not make the material temperature surpass 38 ℃, waits for out the room drying.
5. dry
Material can go out the room drying after cultivating maturation, and when weather is sunny, dry ground on the scene as far as possible, and one can save the energy, and two make microorganism keep more vigor.
During it rain, can only dry, expect temperature control early stage in 38 ℃, and later stage material temperature control is in 45 ℃, and moisture can go out the room less than 10%.
Be semi-finished product after the material drying, experience physical and chemical index and microbiological indicator detect, and reach yeast cells sum 〉=8 * 10
8Individual/gram, active yeast cell sum 〉=6 * 10
8Individual/gram, saccharifying enzymic activity 〉=200U/g, prolease activity 〉=200U/g is enzyme enriched active yeast feed.
Embodiment 3:
1. plant bent and the seed liquor preparation
Tanekoji of Rhizopus oryzae is cultivated
Rhizopus oryzae (Rhizopus oryzae AS 3.866): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences.Enlarge and cultivate flow process: wheat bran test tube (original seed) → triangular flask seed → bent box kind song (wheat bran) → tanekoji of Rhizopus oryzae
Wheat bran adds water 72.5%, 0.12Mpa boiling is 30 minutes under the condition, is cooled to the Rhizopus oryzae that 35 ℃ of inoculations are cultivated through triangular flask, inoculum concentration 0.3%, be incubated 30 ℃ of cultivations, heat up through the germination of 12h bacterial classification, go up bent box and cultivate, total incubation time 33h, can go out the room drying, bake out temperature does not surpass 38 ℃ early stage, and the later stage does not surpass 45 ℃, and moisture 10% is the Rhizopus oryzae sort of quyi.
The geotrichum candidum kind is bent cultivates
Geotrichum candidum (Geotrichum candidum AS 2.361): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences.Enlarge and cultivate flow process: slant tube (original seed) → slant tube → triangular flask seed (brewer's wort) → kind of song
Wheat bran adds water 65%, and boiling is 30 minutes under the boiling 0.12Mpa condition, is cooled to 35 ℃ and connects triangular flask geotrichum candidum seed, and inoculum concentration 0.5% is incubated 34 ℃ of cultivations, and total incubation time 24h can go out the room and dry to moisture 10% and be geotrichum candidum kind song.
Yeast starter liquid is cultivated
Candida utili (Candida utilis ACCC 2102): available from Chinese agriculture microorganism fungus kind preservation administrative center; Candida arborea (Candida arborea AS 2.566): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences; Saccharomyces cerevisiae (Saccharomyces cerevisiae ACCC 2042): available from Chinese agriculture microorganism fungus kind preservation administrative center.
Enlarge and cultivate flow process (three bacterial classification flow process unanimities, cultivate separately): former bacterium test tube → little triangular flask seed (liquid glucose 100ml, 7.5 ° of Be of pol) 30 ℃ of heat insulating culture 11h → change over to big triangular flask (liquid glucose 600ml, 7.5 ° of Be of pol), 30 ℃ of heat insulating culture 11h → change over to (syrup 12.5kg in the Ka Shi jar, 7.5 ° of Be expansion of pol cultivation) 11h → Ka Shi jar yeast starter is spread cultivation at the distiller's yeast cylinder, and (culture medium is that every cylinder is put clear water 250kg in 30 ℃ of cultivations, flour 30kg, wheat bran 10kg) total incubation time 19h is yeast starter liquid.
2. solid-state fermentation culture medium preliminary treatment
With wheat bran 50%, flour 6.5%, vinasse 17.5%, bean cake powder 22.5%, solid-state fermentation culture medium formed by urea 3.5% and water mixes with 1: 0.65 ratio, boiling was sterilized in 35 minutes under 0.12Mpa pressure, close inlet valve after the sterilization, shelving discharging in 35 minutes, with blower fan blast-cold material to 42.5 ℃, transfer PH5.7, in solid-state fermentation culture medium, add again by solid-state fermentation culture medium dry 0.015% AMS (enzyme work is in 5000U/ μ g enzyme, and the enzyme activity determination method is with reference to GB 8275-87), (enzyme work is in 50000U/g for 0.008% carbohydrase, the enzyme activity determination method is with reference to GB 8276-87) and 0.008% cellulase (enzyme work is in 20000U/g, the enzyme activity determination method is with reference to NY/T 912-2004 GB 8276-87), beat evenly with raising the bran machine, carried out preliminary treatment 1.5 hours.
3. inoculation
Through pretreated solid-state fermentation culture medium, ventilation blast-cold to 34 ℃, inoculate (by the culture medium dry) tanekoji of Rhizopus oryzae 1.25% respectively, yeast starter liquid (candida utili seed liquor, Candida arborea seed liquor, saccharomyces cerevisiae seed liquor 15: 15: 1) 12%, geotrichum candidum kind song 0.6% is raised bran machine secondary and is beaten and mix evenly, floating charge level, heat insulating culture, fermentation temperature are controlled at about 30 ℃, voluntarily fermented and cultured.
4. fermentation management
Fermentation temperature has mainly been managed in fermentation.Open temperature-controlled instrument, connect fan power, the temperature upper control limit is 35 ℃, is limited to 33 ℃ down.The diligent observation, diligent test.Temperature controller probe drop point wants correct, the degree of depth will reach 10cm (doing contrast but plug in thermometer on the limit), mainly prevent probe, temperature controller and connection instrument fault, begin after general 6 hours to heat up, wind is touring in the early stage used chamber, and is higher or expect that the temperature rise temperature is too quickly when weather temperature, but air door outside the opening chamber reaches the purpose of regulating growth temperature.Finish through fermentation in 29 hours, can strengthen air door, open door, do not make the material temperature surpass 38 ℃, wait for out the room drying.
5. dry
Material can go out the room drying after cultivating maturation, and when weather is sunny, dry ground on the scene as far as possible, and one can save the energy, and two make microorganism keep more vigor.
During it rain, can only dry, expect temperature control early stage in 38 ℃, and later stage material temperature control is in 45 ℃, and moisture can go out the room less than 10%.
Be semi-finished product after the material drying, experience physical and chemical index and microbiological indicator detect, and reach yeast cells sum 〉=8 * 10
8Individual/gram, active yeast cell sum 〉=6 * 10
8Individual/gram, saccharifying enzymic activity 〉=200U/g, prolease activity 〉=200U/g is enzyme enriched active yeast feed.
Embodiment 4:
1. plant bent and the seed liquor preparation
Tanekoji of Rhizopus oryzae is cultivated
Rhizopus oryzae (Rhizopus oryzae AS 3.866): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences.
Enlarge and cultivate flow process: wheat bran test tube (original seed) → triangular flask seed → bent box kind song (wheat bran) → tanekoji of Rhizopus oryzae
Wheat bran adds water 72.5%, 0.12Mpa boiling is 30 minutes under the condition, is cooled to the Rhizopus oryzae that 35 ℃ of inoculations are cultivated through triangular flask, inoculum concentration 0.3%, be incubated 30 ℃ of cultivations, heat up through the germination of 12h bacterial classification, go up bent box and cultivate, total incubation time 33h, can go out the room drying, bake out temperature does not surpass 38 ℃ early stage, and the later stage does not surpass 45 ℃, and moisture 10% is the Rhizopus oryzae sort of quyi.
The geotrichum candidum kind is bent cultivates
Geotrichum candidum (Geotrichum candidum AS 2.361): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences.Enlarge and cultivate flow process: slant tube (original seed) → slant tube → triangular flask seed (brewer's wort) → kind of song
Wheat bran adds water 65%, and boiling is 30 minutes under the boiling 0.12Mpa condition, is cooled to 35 ℃ and connects triangular flask geotrichum candidum seed, and inoculum concentration 0.5% is incubated 34 ℃ of cultivations, and total incubation time 24h can go out the room and dry to moisture 10% and be geotrichum candidum kind song.
Yeast starter liquid is cultivated
Candida utili (Candida utilis ACCC 2102): available from Chinese agriculture microorganism fungus kind preservation administrative center; Candida arborea (Candida arborea AS 2.566): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences; Saccharomyces cerevisiae (Saccharomyces cerevisiae ACCC 2042): available from Chinese agriculture microorganism fungus kind preservation administrative center.
Enlarge and cultivate flow process (three bacterial classification flow process unanimities, cultivate separately): former bacterium test tube → little triangular flask seed (liquid glucose 100ml, 7.5 ° of Be of pol) 30 ℃ of heat insulating culture 11h → change over to big triangular flask (liquid glucose 600ml, 7.5 ° of Be of pol), 30 ℃ of heat insulating culture 11h → change over to (syrup 12.5kg in the Ka Shi jar, 7.5 ° of Be expansion of pol cultivation) 11h → Ka Shi jar yeast starter is spread cultivation at the distiller's yeast cylinder, and (culture medium is that every cylinder is put clear water 250kg in 30 ℃ of cultivations, flour 30kg, wheat bran 10kg) total incubation time 19h is yeast starter liquid.
2. solid-state fermentation culture medium preliminary treatment
With wheat bran 50%, flour 6.5%, vinasse 17.5%, bean cake powder 22.5%, solid-state fermentation culture medium formed by urea 3.5% and water mixes with 1: 0.65 ratio, boiling was sterilized in 35 minutes under 0.12Mpa pressure, close inlet valve after the sterilization, shelving discharging in 35 minutes, with blower fan blast-cold material to 42.5 ℃, transfer PH5.7, in solid-state fermentation culture medium, add again by solid-state fermentation culture medium dry 0.015% AMS (enzyme work is in 5000U/ μ g enzyme, and the enzyme activity determination method is with reference to GB 8275-87), (enzyme work is in 50000U/g for 0.008% carbohydrase, the enzyme activity determination method is with reference to GB 8276-87) and 0.008% cellulase (enzyme work is in 20000U/g, the enzyme activity determination method is with reference to NY/T 912-2004 GB 8276-87), beat evenly with raising the bran machine, carried out preliminary treatment 1.5 hours.
3. inoculation
Through pretreated solid-state fermentation culture medium, ventilation blast-cold to 34 ℃, inoculate (by the culture medium dry) tanekoji of Rhizopus oryzae 0.5% respectively, yeast starter liquid (candida utili seed liquor, Candida arborea seed liquor, saccharomyces cerevisiae seed liquor 20: 20: 1) 12%, geotrichum candidum kind song 0.8% is raised bran machine secondary and is beaten and mix evenly, floating charge level, heat insulating culture, fermentation temperature are controlled at about 30 ℃, voluntarily fermented and cultured.
4. fermentation management
Fermentation temperature has mainly been managed in fermentation.Open temperature-controlled instrument, connect fan power, the temperature upper control limit is 35 ℃, is limited to 33 ℃ down.The diligent observation, diligent test.Temperature controller probe drop point wants correct, the degree of depth will reach 10cm (doing contrast but plug in thermometer on the limit), mainly prevent probe, temperature controller and connection instrument fault, begin after general 6 hours to heat up, wind is touring in the early stage used chamber, and is higher or expect that the temperature rise temperature is too quickly when weather temperature, but air door outside the opening chamber reaches the purpose of regulating growth temperature.Finish through fermentation in 29 hours, can strengthen air door, open door, do not make the material temperature surpass 38 ℃, wait for out the room drying.
5. dry
Material can go out the room drying after cultivating maturation, and when weather is sunny, dry ground on the scene as far as possible, and one can save the energy, and two make microorganism keep more vigor.
During it rain, can only dry, expect temperature control early stage in 38 ℃, and later stage material temperature control is in 45 ℃, and moisture can go out the room less than 10%.
Be semi-finished product after the material drying, experience physical and chemical index and microbiological indicator detect, and reach yeast cells sum 〉=8 * 10
8Individual/gram, active yeast cell sum 〉=6 * 10
8Individual/gram, saccharifying enzymic activity 〉=200U/g, prolease activity 〉=200U/g is enzyme enriched active yeast feed.
Embodiment 5:
1. plant bent and the seed liquor preparation
Tanekoji of Rhizopus oryzae is cultivated
Rhizopus oryzae (Rhizopus oryzae AS 3.866): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences.Enlarge and cultivate flow process: wheat bran test tube (original seed) → triangular flask seed → bent box kind song (wheat bran) → tanekoji of Rhizopus oryzae
Wheat bran adds water 72.5%, 0.12Mpa boiling is 30 minutes under the condition, is cooled to the Rhizopus oryzae that 35 ℃ of inoculations are cultivated through triangular flask, inoculum concentration 0.3%, be incubated 30 ℃ of cultivations, heat up through the germination of 12h bacterial classification, go up bent box and cultivate, total incubation time 33h, can go out the room drying, bake out temperature does not surpass 38 ℃ early stage, and the later stage does not surpass 45 ℃, and moisture 10% is the Rhizopus oryzae sort of quyi.
The geotrichum candidum kind is bent cultivates
Geotrichum candidum (Geotrichum candidum AS 2.361): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences.Enlarge and cultivate flow process: slant tube (original seed) → slant tube → triangular flask seed (brewer's wort) → kind of song
Wheat bran adds water 65%, and boiling is 30 minutes under the boiling 0.12Mpa condition, is cooled to 35 ℃ and connects triangular flask geotrichum candidum seed, and inoculum concentration 0.5% is incubated 34 ℃ of cultivations, and total incubation time 24h can go out the room and dry to moisture 10% and be geotrichum candidum kind song.
Yeast starter liquid is cultivated
Candida utili (Candida utilis ACCC 2102): available from Chinese agriculture microorganism fungus kind preservation administrative center; Candida arborea (Candida arborea AS 2.566): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences; Saccharomyces cerevisiae (Saccharomyces cerevisiae ACCC 2042): available from Chinese agriculture microorganism fungus kind preservation administrative center.
Enlarge and cultivate flow process (three bacterial classification flow process unanimities, cultivate separately): former bacterium test tube → little triangular flask seed (liquid glucose 100ml, 7.5 ° of Be of pol) 30 ℃ of heat insulating culture 11h → change over to big triangular flask (liquid glucose 600ml, 7.5 ° of Be of pol), 30 ℃ of heat insulating culture 11h → change over to (syrup 12.5kg in the Ka Shi jar, 7.5 ° of Be expansion of pol cultivation) 11h → Ka Shi jar yeast starter is spread cultivation at the distiller's yeast cylinder, and (culture medium is that every cylinder is put clear water 250kg in 30 ℃ of cultivations, flour 30kg, wheat bran 10kg) total incubation time 19h is yeast starter liquid.
2. solid-state fermentation culture medium preliminary treatment
With wheat bran 50%, flour 6.5%, vinasse 17.5%, bean cake powder 22.5%, solid-state fermentation culture medium formed by urea 3.5% and water mixes with 1: 0.65 ratio, boiling was sterilized in 35 minutes under 0.12Mpa pressure, close inlet valve after the sterilization, shelving discharging in 35 minutes, with blower fan blast-cold material to 42.5 ℃, transfer PH5.7, in solid-state fermentation culture medium, add again by solid-state fermentation culture medium dry 0.015% AMS (enzyme work is in 5000U/ μ g enzyme, and the enzyme activity determination method is with reference to GB 8275-87), (enzyme work is in 50000U/g for 0.008% carbohydrase, the enzyme activity determination method is with reference to GB 8276-87) and 0.008% cellulase (enzyme work is in 20000U/g, the enzyme activity determination method is with reference to NY/T 912-2004 GB 8276-87), beat evenly with raising the bran machine, carried out preliminary treatment 1.5 hours.
3. inoculation
Through pretreated solid-state fermentation culture medium, ventilation blast-cold to 34 ℃, inoculate (by the culture medium dry) tanekoji of Rhizopus oryzae 2% respectively, yeast starter liquid (candida utili seed liquor, Candida arborea seed liquor, saccharomyces cerevisiae seed liquor 10: 10: 1) 12%, geotrichum candidum kind song 0.8% is raised bran machine secondary and is beaten and mix evenly, floating charge level, heat insulating culture, fermentation temperature are controlled at about 30 ℃, voluntarily fermented and cultured.
4. fermentation management
Fermentation temperature has mainly been managed in fermentation.Open temperature-controlled instrument, connect fan power, the temperature upper control limit is 35 ℃, is limited to 33 ℃ down.The diligent observation, diligent test.Temperature controller probe drop point wants correct, the degree of depth will reach 10cm (doing contrast but plug in thermometer on the limit), mainly prevent probe, temperature controller and connection instrument fault, begin after general 6 hours to heat up, wind is touring in the early stage used chamber, and is higher or expect that the temperature rise temperature is too quickly when weather temperature, but air door outside the opening chamber reaches the purpose of regulating growth temperature.Finish through fermentation in 29 hours, can strengthen air door, open door, do not make the material temperature surpass 38 ℃, wait for out the room drying.
5. dry
Material can go out the room drying after cultivating maturation, and when weather is sunny, dry ground on the scene as far as possible, and one can save the energy, and two make microorganism keep more vigor.
During it rain, can only dry, expect temperature control early stage in 38 ℃, and later stage material temperature control is in 45 ℃, and moisture can go out the room less than 10%.
Be semi-finished product after the material drying, experience physical and chemical index and microbiological indicator detect, and reach yeast cells sum 〉=8 * 10
8Individual/gram, active yeast cell sum 〉=6 * 10
8Individual/gram, saccharifying enzymic activity 〉=200U/g, prolease activity 〉=200U/g is enzyme enriched active yeast feed.
Embodiment 6:
1. plant bent and the seed liquor preparation
Tanekoji of Rhizopus oryzae is cultivated
Rhizopus oryzae (Rhizopus oryzae AS 3.866): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences.Enlarge and cultivate flow process: wheat bran test tube (original seed) → triangular flask seed → bent box kind song (wheat bran) → tanekoji of Rhizopus oryzae
Wheat bran adds water 72.5%, 0.12Mpa boiling is 30 minutes under the condition, is cooled to the Rhizopus oryzae that 35 ℃ of inoculations are cultivated through triangular flask, inoculum concentration 0.3%, be incubated 30 ℃ of cultivations, heat up through the germination of 12h bacterial classification, go up bent box and cultivate, total incubation time 33h, can go out the room drying, bake out temperature does not surpass 38 ℃ early stage, and the later stage does not surpass 45 ℃, and moisture 10% is the Rhizopus oryzae sort of quyi.
The geotrichum candidum kind is bent cultivates
Geotrichum candidum (Geotrichum candidum AS 2.361): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences.Enlarge and cultivate flow process: slant tube (original seed) → slant tube → triangular flask seed (brewer's wort) → kind of song
Wheat bran adds water 65%, and boiling is 30 minutes under the boiling 0.12Mpa condition, is cooled to 35 ℃ and connects triangular flask geotrichum candidum seed, and inoculum concentration 0.5% is incubated 34 ℃ of cultivations, and total incubation time 24h can go out the room and dry to moisture 10% and be geotrichum candidum kind song.
Yeast starter liquid is cultivated
Candida utili (Candida utilis ACCC 2 102): available from Chinese agriculture microorganism fungus kind preservation administrative center; Candida arborea (Candida arborea AS 2.566): available from DSMZ of Institute of Micro-biology of the Chinese Academy of Sciences; Saccharomyces cerevisiae (Saccharomyces cerevisiae ACCC 2042): available from Chinese agriculture microorganism fungus kind preservation administrative center.
Enlarge and cultivate flow process (three bacterial classification flow process unanimities, cultivate separately): former bacterium test tube → little triangular flask seed (liquid glucose 100ml, 7.5 ° of Be of pol) 30 ℃ of heat insulating culture 11h → change over to big triangular flask (liquid glucose 600ml, 7.5 ° of Be of pol), 30 ℃ of heat insulating culture 11h → change over to (syrup 12.5kg in the Ka Shi jar, 7.5 ° of Be expansion of pol cultivation) 11h → Ka Shi jar yeast starter is spread cultivation at the distiller's yeast cylinder, and (culture medium is that every cylinder is put clear water 250kg in 30 ℃ of cultivations, flour 30kg, wheat bran 10kg) total incubation time 19h is yeast starter liquid.
2. solid-state fermentation culture medium preliminary treatment
With wheat bran 50%, flour 6.5%, vinasse 17.5%, bean cake powder 22.5%, solid-state fermentation culture medium formed by urea 3.5% and water mixes with 1: 0.65 ratio, boiling was sterilized in 35 minutes under 0.12Mpa pressure, close inlet valve after the sterilization, shelving discharging in 35 minutes, with blower fan blast-cold material to 42.5 ℃, transfer PH5.7, in solid-state fermentation culture medium, add again by solid-state fermentation culture medium dry 0.015% AMS (enzyme work is in 5000U/ μ g enzyme, and the enzyme activity determination method is with reference to GB 8275-87), (enzyme work is in 50000U/g for 0.008% carbohydrase, the enzyme activity determination method is with reference to GB 8276-87) and 0.008% cellulase (enzyme work is in 20000U/g, the enzyme activity determination method is with reference to NY/T 912-2004 GB 8276-87), beat evenly with raising the bran machine, carried out preliminary treatment 1.5 hours.
3. inoculation
Through pretreated solid-state fermentation culture medium, ventilation blast-cold to 34 ℃, inoculate (by the culture medium dry) tanekoji of Rhizopus oryzae 2% respectively, yeast starter liquid (candida utili seed liquor, Candida arborea seed liquor, saccharomyces cerevisiae seed liquor 20: 20: 1) 12%, geotrichum candidum kind song 0.4% is raised bran machine secondary and is beaten and mix evenly, floating charge level, heat insulating culture, fermentation temperature are controlled at about 30 ℃, voluntarily fermented and cultured.
4. fermentation management
Fermentation temperature has mainly been managed in fermentation.Open temperature-controlled instrument, connect fan power, the temperature upper control limit is 35 ℃, is limited to 33 ℃ down.The diligent observation, diligent test.Temperature controller probe drop point wants correct, the degree of depth will reach 10cm (doing contrast but plug in thermometer on the limit), mainly prevent probe, temperature controller and connection instrument fault, begin after general 6 hours to heat up, wind is touring in the early stage used chamber, and is higher or expect that the temperature rise temperature is too quickly when weather temperature, but air door outside the opening chamber reaches the purpose of regulating growth temperature.Finish through fermentation in 29 hours, can strengthen air door, open door, do not make the material temperature surpass 38 ℃, wait for out the room drying.
5. dry
Material can go out the room drying after cultivating maturation, and when weather is sunny, dry ground on the scene as far as possible, and one can save the energy, and two make microorganism keep more vigor.
During it rain, can only dry, expect temperature control early stage in 38 ℃, and later stage material temperature control is in 45 ℃, and moisture can go out the room less than 10%.
Be semi-finished product after the material drying, experience physical and chemical index and microbiological indicator detect, and reach yeast cells sum 〉=8 * 10
8Individual/gram, active yeast cell sum 〉=6 * 10
8Individual/gram, saccharifying enzymic activity 〉=200U/g, prolease activity 〉=200U/g is enzyme enriched active yeast feed.
Application example
In triennium in by the end of October, 2004, this seminar is plant of family surplus priority Zhejiang Hangzhou, Ningbo, Jinhua etc. 10, compares test with enzyme enriched active yeast of the present invention, and the lab analysis of physical and chemical index and productivity application show:
Application example 1: enzyme enriched active yeast is to the influence of 0-28 age in days meat chicken production performance
| Group | Control group | Test group |
| The chicken number | 150 | 150 |
| Average starting weight (g) | 39.83±0.64 a | 40.87±1.8 a |
| Average end heavy (g) | 586.5±105.6 a | 622.7±80.6 a |
| Average daily gain (g) | 19.40±3.77 a | 20.75±2.88 a |
| The material anharmonic ratio | 2.15±0.01 a | 2.03±0.06 b |
| Survival rate (%) | 92.0±2.00 a | 95.3±3.06 a |
The result shows: the 0-28 age in days, the test group daily ration substitutes 3% dregs of beans with 3% high-activity yeast, and the average end of test group is heavy, average daily gain, survival rate improve 6.17% (P＞0.05), 6.96% (P＞0.05) and 3.59% (P＞0.05) than control group respectively.Test group material anharmonic ratio is than control group decline 5.58% (P＜0.05).
Application example 2: enzyme enriched active yeast is to the influence of 0-49 age in days meat chicken production performance
| Group | Control group | Test group |
| The chicken number | 150 | 150 |
| Average starting weight (g) | 39.83±0.64 a | 40.87±1.8 a |
| Average end heavy (g) | 1497.0±202.6 a | 1627.8±182.3 b |
| Average daily gain (g) | 29.74±3.77 a | 32.39±2.88 b |
| The material anharmonic ratio | 2.46±0.08 a | 2.34±0.09 a |
| Survival rate | 92.0±2.00 a | 95.3±3.06 a |
The result shows, the 0-49 age in days, the test group daily ration substitutes 3% dregs of beans with 3% high-activity yeast, and the average end of test group is heavy, average daily gain, survival rate improve 8.74% (P＜0.05), 8.91% (P＜0.05) and 3.59% (P＞0.05) than control group respectively.Test group material anharmonic ratio is than control group decline 4.88% (P＞0.05).
Application example 3: the active yeast of complex enzyme is to the influence of sow reproductive performance
| Produce the young number of living | Nascent litter weight (Kg) | Birth weight (Kg) | |
| Control group | 10.0±1.4 | 12.56±1.32 | 1.26±0.10 |
| Test group | 10.7±1.0 | 14.71±1.58 | 1.38±0.15 |
By table as seen, the active yeast of complex enzyme has certain influence to sow reproductive performance.Produce the young number of living, newborn piglet litter weight and the comparison of piglet birth weight test group and you can well imagine high by 7.0% (P＞0.05), 17.12% (P＞0.05) and 9.52% (P＞0.05) according to component.
The active yeast of complex enzyme is to the influence of sucking pig production performance
Application example 4: the active yeast of complex enzyme is to the influence of sucking pig production performance
| Wean counterpoise (Kg) | Daily gain (g) | Survival rate (%) | |
| Control group | 8.02±0.24 | 242±16 | 92.37±0.07 |
| Test group | 8.58±0.17 | 257±21 | 95.48±0.05 |
Can be seen Table by table: the active yeast of complex enzyme has certain influence to the sucking pig production performance.Wean counterpoise, daily gain and the comparison of survival rate test group be you can well imagine high by 6.98% (P＞0.05), 6.20% (P＞0.05) and 3.37% (P＞0.05) according to component.
Application example 5: enzyme enriched active yeast is to the influence of daily ration total energy and crude protein apparent digestibility
| Group | Control group | Test group I | Test group II |
| The total energy apparent digestibility | 77.17±1.05 a | 81.79±1.56 b | 76.95±0.94 |
| Thick protein apparent digestibility (%) | 72.98±1.63 a | 77.25±1.02 b | 72.67±1.23 a |
By table as seen, the application enzyme enriched active yeast has appreciable impact to the apparent digestibility of test pig daily ration total energy and crude protein.Compare with common yeast group with control group, the apparent digestibility of daily ration total energy improves 6.0% (P＜0.05) and 6.3% (P＜0.05) respectively, and the apparent digestibility of crude protein improves 5.9% (P＜0.05) and 6.3% (P＜0.05) respectively.Control group and common yeast group no significant difference.
Application example 6: enzyme enriched active yeast is to the influence of test pig production performance
| Group | Control group | Test group I | Test group II |
| Starting weight (Kg) | 21.12±0.23 a | 21.08±0.18 a | 20.97±0.31 a |
| End heavy (Kg) | 38.43±0.81 a | 39.77±0.58 a | 38.44±0.73 a |
| Average daily gain (g) | 540±29 a | 586±37 b | 546±41 a |
| Average daily ingestion amount (g) | 1402±39 a | 1454±43 a | 1426±33 a |
| The material anharmonic ratio | 2.59±0.19 a | 2.37±0.14 a | 2.61±0.23 a |
| Diarrhea rate (%) | 6.61±1.21 a | 3.70±0.92 b | 6.08±0.92 a |
By table 2-2 as seen: use enzyme enriched active yeast and bring up again high by 3.5% (P＞0.05) and 3.5% (P＞0.05) than the average end of control group and common yeast group; Average daily gain improves 8.5% (P＜0.05) and 7.3% (P＜0.05); Material anharmonic ratio descended 8.5% (P＜0.05) (P＞0.05) and 9.2% (P＜0.05); Test pig diarrhea frequency descend respectively 44.0% (P＜0.05) and 39.1% (P＜0.05).Production performance no significant difference between control group and common yeast group.
Claims (1)
1. the preparation method of an enzyme enriched active yeast feed, it is characterized in that Rhizopus oryzae is made tanekoji of Rhizopus oryzae, geotrichum candidum is made geotrichum candidum kind song, with candida utili, Candida arborea and saccharomyces cerevisiae are made respectively after the seed liquor in 20-10: 20-10: 1 ratio is mixed and made into yeast starter liquid, again tanekoji of Rhizopus oryzae is pressed 0.5～2% of solid-state fermentation culture medium, yeast starter liquid is by 12% of solid-state fermentation culture medium, geotrichum candidum kind song is inoculated in pretreated solid-state fermentation culture medium by 0.4～0.8% of solid-state fermentation culture medium, at 33-35 ℃ of fermented and cultured 28-30 hour after dry or 38～45 ℃ of dryings, reach moisture＜10%, yeast cells sum 〉=8 * 108/gram, active yeast cell sum 〉=6 * 108/gram, saccharifying enzymic activity 〉=200U/g, prolease activity 〉=200U/g is enzyme enriched active yeast feed; Wherein solid-state fermentation culture medium is: wheat bran 40-60%, flour 3-10%, vinasse 15-20%, bean cake powder 20-25%, the mixture that urea 2-5% forms; The preprocess method that solid state fermentation is cultivated is: with solid-state fermentation culture medium and water with 1: the 0.6-0.7 ratio mixes, sterilization in boiling 30-40 minute under 0.1-0.15Mpa pressure, in solid-state fermentation culture medium, add again, carried out preliminary treatment 1-2 hour by solid-state fermentation culture medium dry 0.01%-0.02% AMS, 0.005%-0.01% carbohydrase and 0.005%-0.01% cellulase.
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| CN100405922C (en) * | 2005-10-26 | 2008-07-30 | 孙荣高 | Method for producing thallus protein forage by using solid state fermentation of pomace and dreg or stalks with mixed aerobic bacteria liquor |
| CN101785524B (en) * | 2010-02-10 | 2012-09-05 | 湖北高生生物饲料有限公司 | Yeast protein feed using spirit-based distillers' grains as base material and production method thereof |
| CN102511650B (en) * | 2011-12-07 | 2013-09-18 | 河南科技大学 | Method for preparing protein feed by using Jerusalem artichoke residues |
| CN102899259A (en) * | 2012-10-15 | 2013-01-30 | 桐乡市五丰饲料有限公司 | Preparation method and use of alpha saccharomyces cerevisiae |
| CN103689224B (en) * | 2013-12-07 | 2015-09-30 | 路德环境科技股份有限公司 | A kind of Zymolytic spirit vinasse high-peptide feed and production method |
| CN103749948A (en) * | 2013-12-30 | 2014-04-30 | 黑龙江省轻工科学研究院 | Method for manufacturing fermented feed |
| CN104171410B (en) * | 2014-08-03 | 2016-08-17 | 刘骏 | A kind of manufacture method of cow feed additive |
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| JPS62215351A (en) * | 1986-03-16 | 1987-09-22 | Toyo Baamikiyuraito Kk | Additive containing vermiculite as substrate for domestic animal feed |
| JPH09169587A (en) * | 1995-12-20 | 1997-06-30 | Taihei Bussan Kk | Production of rice bran fermentation product containing a large quantity of yeast and rice bran fermentation product containing a large quantity of yeast obtained by the same |
| US20040023858A1 (en) * | 2000-08-14 | 2004-02-05 | Fumiaki Ikeda | Antifungal combination therapy |
| CN1570082A (en) * | 2004-04-27 | 2005-01-26 | 包头市天赋食品有限公司 | Multiple strain microorganism ferment production method and its uses in cow fine fodder |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62215351A (en) * | 1986-03-16 | 1987-09-22 | Toyo Baamikiyuraito Kk | Additive containing vermiculite as substrate for domestic animal feed |
| JPH09169587A (en) * | 1995-12-20 | 1997-06-30 | Taihei Bussan Kk | Production of rice bran fermentation product containing a large quantity of yeast and rice bran fermentation product containing a large quantity of yeast obtained by the same |
| US20040023858A1 (en) * | 2000-08-14 | 2004-02-05 | Fumiaki Ikeda | Antifungal combination therapy |
| CN1570082A (en) * | 2004-04-27 | 2005-01-26 | 包头市天赋食品有限公司 | Multiple strain microorganism ferment production method and its uses in cow fine fodder |
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