CN101785524B - Yeast protein feed using spirit-based distillers' grains as base material and production method thereof - Google Patents
Yeast protein feed using spirit-based distillers' grains as base material and production method thereof Download PDFInfo
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Abstract
The invention relates to a yeast protein feed using spirit-based distillers' grains as base material and a production method thereof. The yeast protein feed uses the spirit-based distillers' grains as the base material, materials for fermentation culture are added and comprise enzyme preparation used for fermentation of the distillers' grains, yeast inoculant and nutrition auxiliary materials for heat preservation culture after being mixed, and finally the product is prepared by drying. The product per gram contains 2-5 billion yeasts. The invention has the advantages that the production method is a production method of microecological feed with no pollution. The feed can improve the digestive absorption of livestock and poultry for the nutrient substances, improves the digestive function, promotes the production performance of the livestock and poultry and enhances the immunity; meanwhile, the feed also improves the nutritional value of the spirit-based distillers' grains and becomes the novel protein feed which turn waste into wealth.
Description
Technical field
The present invention relates to a kind of is the Yeast protein feed and the production method thereof of base-material with the distillers ' grains, belongs to yeast cells solid-state fermentation technology field.
Background technology
The millions of approximately tons of China's liquor annual production, accessory substance-distillers ' grains (wetting) that liquor is produced is above ten million ton.Liquor is used Chinese sorghum, corn, and wheat, paddy, single raw material of pure grain such as barley or mixed material fermentation form.Liquor fermentation mainly is that the starch in the raw material is transformed, and less to the protein component trans-utilization in the raw material, they are kept in the vinasse basically.Since the fermentation inspissation (raw starch is utilized), the protein content of vinasse (butt) is compared with raw material, can double or more than, reach about 20%.Outside isolating protein content was higher, the amino acid ratio of components of vinasse protein was a full price than balance basically; Vinasse mineral content is also very abundant, calcium wherein, and main trace element content such as iron are than higher; In addition, also contain multivitamin, and the various bioactivators (like purine, pyrimidine, lipids, enzyme) of thalline self-dissolving generation etc.But distillers ' grains also exists defective and the obstacle of using in the tangible nutrition: 1, fresh grain stillage moisture is big, and acidity is high, and is perishable rotten, is difficult for keeping and transportation, and a large amount of distillers ' grains is stacked serious environment pollution arbitrarily; 2, directly extremely cheap on selling price as feed, cause the waste of economic worth; 3, contain in the distillers ' grains and be difficult to the rice husk (mainly containing crude fibre, lignin) that digests about 50%, the livestock and poultry of directly feeding influence digesting and assimilating of animal.At present, have only the sub-fraction distillers ' grains as feed or fertilizer, major part is regarded as refuse, if directly discharging is the wasting of resources, causes environmental pollution again.
" directly microorganism fodder " (DFM) is meant that microbial cell can directly feed to a kind of product of animal.These microorganisms comprise bacterium, yeast (fungi) etc.Increasing DFM is used, and is the result that people require to reduce antibiotic and other growth promotion drug use amount.Simultaneously, many evidences show that the DFM result of use is good, Yeast protein feed especially, it through the microorganism in living with animal gastrointestinal tract provide extra nutrition substrate stimulate their metabolic activities and safeguard in ecological environment relatively stable.Many products (like beneficial health X P, Yea Sail etc.) have come into the market, but prior art is produced very costliness of feed price with a large amount of soybean proteins, fish meal protein etc.Because of having many difficulties on the distillers ' grains production yeast feed protein technology, the product that is difficult to obtain does not also utilize distillers ' grains to produce the yeast feed protein successful examples for a long time.
Summary of the invention
First purpose of the present invention is: providing a kind of is the Yeast protein feed of base-material with the distillers ' grains, specifically: through the inoculation yeast inoculum, utilize distillers ' grains to carry out solid state fermentation and obtain high density yeast cells thalline.It is a kind of production method of micro-ecological feed, and this feed can improve livestock and poultry to the digesting and assimilating of nutriment, and improves digestive function, promotes the livestock and poultry production performance performance, and can strengthen its immunity.Improved simultaneously the nutritive value of distillers ' grains again, made it become novel protein feed, turned waste into wealth.
Another object of the present invention is: providing a kind of is the Yeast protein feed production method of base-material with the distillers ' grains.
The present invention is that the Yeast protein feed technical scheme of base-material is with the distillers ' grains:
A kind of is the Yeast protein feed of base-material with the distillers ' grains, is to be base-material with the distillers ' grains, and add the material of participating in fermented and cultured and comprise: distillers ' grains ferments with enzyme preparation, yeast-inoculated agent and nutrition accessory, heat insulating culture after mixing, and product is processed in oven dry at last; Every gram product contains saccharomycete 20～5,000,000,000.
Further technical scheme is:
Described is the Yeast protein feed of base-material with the distillers ' grains, and its every gram product contains saccharomycete 25～4,000,000,000.
The present invention is that the technical scheme of the Yeast protein feed production method of base-material is with the distillers ' grains:
A kind of is the Yeast protein feed production method of base-material with the distillers ' grains; With the distillers ' grains is base-material; Adding the material of participating in fermented and cultured comprises: distillers ' grains ferments with enzyme preparation, yeast-inoculated agent and nutrition accessory, heat insulating culture after mixing, and product is processed in oven dry at last; Every gram product contains saccharomycete 20～5,000,000,000; It is following that its concrete system is joined step:
(1) select distillers ' grains for use: take by weighing the fresh white vinasse as base-material, measure its water content, calculate the weight of extra dry white wine vinasse, the weight by the extra dry white wine vinasse adds following materials then:
The enzyme preparation that (2) will be used for distillers ' grains fermentation joins the fresh white vinasse by extra dry white wine vinasse weight proportion, mixes then, obtains medium matrix;
(3) the yeast-inoculated agent is joined by in the extra dry white wine vinasse weight proportion medium matrix;
(4) adjustment water content; By the above-mentioned the 1st) amount of step extra dry white wine vinasse calculates amount of water, the adjustment house water yield;
(5) initial heat insulating culture;
(6) add behind the nutrition accessory heat insulating culture again: the Ensure Liquid auxiliary material is for once adding, or the gradation adding, adds behind the auxiliary material heat insulating culture more at every turn; Add heat insulating culture a period of time behind the auxiliary material at every turn;
(7) oven dry and making finished product: the material of final heat insulating culture is dried, pulverized or granulation or film-making with conventional method, and promptly obtaining with the distillers ' grains is the Yeast protein feed finished product of base-material.
Further technical scheme is:
Described is the Yeast protein feed production method of base-material with the distillers ' grains, and its enzyme preparation that is used for the distillers ' grains fermentation is a kind of hydrolase preparation or two or more composite hydrolysis enzyme preparation of cellulase or hemicellulase or pectase; Said yeast-inoculated agent is to be fit to the barms of distillers ' grains fermented yeast after suitable enlarged culture; Said nutrition accessory comprises molasses and/or nitrogenous auxiliary material, and said nitrogenous auxiliary material is urea or sulfate of ammoniac or ammonium hydrogencarbonate or ammonium carbonate or ammonium phosphate, MAP, Diammonium phosphate (DAP) wherein one or both or two or more mixture.
Described is the Yeast protein feed production method of base-material with the distillers ' grains, described barms is barms that to be bacterial classification with the brewer's yeast be prepared from through one-level or secondary enlarged culture; Said distillers ' grains is the poor and/or distills ' grains of Xiaoqu wine.
Described is the Yeast protein feed production method of base-material with the distillers ' grains, and said distillers ' grains fermentation uses the enzyme preparation addition to be extra dry white wine vinasse weight 0.1%～5%; Said yeast-inoculated agent adds by the amount that every gram extra dry white wine vinasse contain saccharomycete 0.1～1.0 hundred million; Said adjustment water content is to add water adjustment distillers ' grains water content to 35%～65%; The temperature of initial heat insulating culture is 28～30 ℃, and the heat insulating culture time is 30～45 hours; Said nutrition accessory addition is: the molasses addition is 4～10% of an extra dry white wine vinasse weight, and/or nitrogenous auxiliary material addition by nitrogen content count per kilogram extra dry white wine vinasse contain 0.25～0.8 the gram purity nitrogen; The heat insulating culture time is 45～55 hours behind the Ensure Liquid auxiliary material for the first time, and adding the auxiliary material heat insulating culture time for the second time is 65～75 hours, and twice holding temperature is 28～30 ℃.
Described is the Yeast protein feed production method of base-material with the distillers ' grains, the steps include:
(1) take by weighing distillers ' grains be fresh Xiaoqu wine poor 2000 kilograms as base-material: measuring its water content is 50%, and the weight that calculates the extra dry white wine vinasse is 1000 kilograms, and the weight by the extra dry white wine vinasse adds following materials then:
The enzyme preparation that (2) will be used for distillers ' grains fermentation by extra dry white wine vinasse weight 4%, promptly 40 kilograms, join in the fresh white vinasse, mix the acquisition medium matrix then; Said enzyme preparation is the composite hydrolysis enzyme preparation of cellulase, hemicellulase, pectase;
(3) the yeast-inoculated agent is contained 0.6 hundred million proportionings of saccharomycete, is that the access amount of yeast-inoculated agent is 60 kilograms and joins in above-mentioned (2) the step medium matrix by every gram extra dry white wine vinasse; Said yeast-inoculated agent is to be the barms that bacterial classification is prepared from through the secondary enlarged culture with the brewer's yeast;
(4) behind above-mentioned (3) EOS, press the above-mentioned the 1st) step extra dry white wine vinasse adjustment, realize that moisture distillers ' grains water content is 55%; Moisture because of the fresh white vinasse, add water only and then be 222.2 kilograms;
(5) initial heat insulating culture: under 29 ℃ of conditions, carried out initial heat insulating culture 30 hours after adjusting water content;
(6) add behind the nutrition accessory heat insulating culture again: initial heat insulating culture is accomplished the 4% adding molasses that the extra dry white wine vinasse are pressed in the back; Promptly add 40 kilograms, add the auxiliary material that contains 0.25 gram purity nitrogen by per kilogram extra dry white wine vinasse simultaneously, promptly contain the nitrogenous auxiliary material of purity nitrogen 250 grams altogether; Preferred urea, addition are 543 grams; Heat insulating culture 51 hours again after the adding adds auxiliary material more for the second time, and addition is identical with the first time; And then heat insulating culture end in 72 hours, twice holding temperature range of choice is 28～30 ℃;
(7) oven dry and making finished product: powdery is dried, is ground into to the material of final heat insulating culture by conventional method, and promptly obtaining with the distillers ' grains is the Yeast protein feed finished product of base-material; Every gram product contains saccharomycete 45～5,000,000,000.
Described is the Yeast protein feed production method of base-material with the distillers ' grains, the steps include:
(1) take by weighing distillers ' grains, mix totally 1818 kilograms as base-material for fresh Xiaoqu wine is poor with distills ' grains: measuring its water content is 45%, and the weight that calculates the extra dry white wine vinasse is 1000 kilograms, and the weight by the extra dry white wine vinasse adds following materials then:
The enzyme preparation that (2) will be used for distillers ' grains fermentation by extra dry white wine vinasse weight 5%, promptly 50 kilograms, join in the fresh white vinasse, mix the acquisition medium matrix then; Said enzyme preparation is the cellulose hydrolyzation enzyme preparation;
(3) the yeast-inoculated agent is contained 100,000,000 proportionings of saccharomycete by every gram extra dry white wine vinasse and join in above-mentioned (2) the step medium matrix, the access amount of yeast-inoculated agent is 100 kilograms; Said yeast-inoculated agent is to be the barms that bacterial classification is prepared from through the one-level enlarged culture with the brewer's yeast;
(4) finish the back by the above-mentioned the 1st above-mentioned (3)) step extra dry white wine vinasse adjustment, moisture distillers ' grains water content is 60%, and is moisture because of the fresh white vinasse, adds water only and then is 682 kilograms;
(5) initial heat insulating culture: under 28 ℃ of conditions, carried out initial heat insulating culture 45 hours after adjusting water content;
(6) add behind the nutrition accessory heat insulating culture again: initial heat insulating culture is accomplished the 10% adding molasses that the extra dry white wine vinasse are pressed in the back; Promptly add 100 kilograms, and then heat insulating culture 55 hours hours, add nitrogenous auxiliary material at last again; Add the auxiliary material that contains 0.8 gram purity nitrogen by per kilogram extra dry white wine vinasse; The nitrogenous auxiliary material that promptly contains purity nitrogen 800 grams altogether selects ammonium sulfate to add, and amounting to addition is 3810 grams. and then heat insulating culture finished in 75 hours; Can also select urea, or ammonium hydrogencarbonate, ammonium carbonate, ammonium phosphate comprises MAP, Diammonium phosphate (DAP), perhaps they is mixed and adds; When selecting urea for use, addition is 1739 grams; Every gram product contains saccharomycete 30～3,500,000,000.
(7) oven dry and making finished product: the system minitype particle is dried, pulverized to the material of final heat insulating culture by conventional method, and promptly obtaining with the distillers ' grains is the Yeast protein feed finished product of base-material.
Described is the Yeast protein feed production method of base-material with the distillers ' grains, and its distillers ' grains fermentation uses enzyme preparation to be 4% of extra dry white wine vinasse weight; Add water adjustment distillers ' grains water content to 35%; The temperature of initial heat insulating culture is 30 ℃, and the heat insulating culture time is 45 hours; Said nutrition accessory addition is: the molasses addition is 5% of an extra dry white wine vinasse weight, and nitrogenous auxiliary material addition is counted per kilogram extra dry white wine vinasse by nitrogen content and contained 0.5 gram purity nitrogen; The every gram product of Yeast protein feed contains saccharomycete 20～3,000,000,000.
Described is the Yeast protein feed production method of base-material with the distillers ' grains, and its distillers ' grains fermentation uses enzyme preparation to be 0.1% of extra dry white wine vinasse weight; The yeast-inoculated agent contains 0.1 hundred million proportionings of saccharomycete by every gram extra dry white wine vinasse; Distillers ' grains is fresh distills ' grains; Nutrition accessory comprises molasses and nitrogenous auxiliary material, and molasses are 6% of extra dry white wine vinasse weight, and its nitrogen content of nitrogenous auxiliary material is that per kilogram extra dry white wine vinasse contain 0.3 gram purity nitrogen; The every gram product of Yeast protein feed contains saccharomycete 30～3,500,000,000.
Method advantage of the present invention is:
1, the loose ventilative characteristics that are suitable as solid fermentation matrix and the suitable yeast conversion of distillers ' grains nutrition of distillers ' grains have been utilized; With the distillers ' grains is the technology of the yeast high density solid fermentation of matrix; Be abundant excavation and the utilization that vinasse are worth, the demand that satisfies aquaculture and farming and animal husbandry is had crucial meaning;
2, development product has wide market prospects and higher added value is arranged, but nearly 1000 yuan of vinasse new returns per ton,
3, have positive environmental benefit, this discarded object of vinasse is fully used, reduced pollution environment;
The specific embodiment
Is that the Yeast protein feed and the production method thereof of base-material is described further as follows in conjunction with embodiment with the distillers ' grains to the present invention:
Embodiment 1: the present invention is that the Yeast protein feed technical scheme of base-material is with the distillers ' grains: be base-material with the distillers ' grains; Adding the material of participating in fermented and cultured comprises: distillers ' grains ferments with enzyme preparation, yeast-inoculated agent and nutrition accessory; Heat insulating culture after mixing, product is processed in oven dry at last; Every gram product contains saccharomycete 20～5,000,000,000.
Embodiment 2: different with the foregoing description is: described is the Yeast protein feed of base-material with the distillers ' grains, and every gram product contains saccharomycete 25～4,000,000,000.
Embodiment 3: a kind of is the Yeast protein feed production method of base-material with the distillers ' grains; It is to be base-material with the distillers ' grains; Adding the material of participating in fermented and cultured has: distillers ' grains ferments with enzyme preparation, yeast-inoculated agent and nutrition accessory, heat insulating culture after mixing, and product is processed in oven dry at last; Every gram product contains saccharomycete 20～5,000,000,000; It is following that its concrete system is joined step:
(1) select distillers ' grains for use: take by weighing the fresh white vinasse as base-material, measure its water content, calculate the weight of extra dry white wine vinasse, the weight by the extra dry white wine vinasse adds following materials then:
The enzyme preparation that (2) will be used for distillers ' grains fermentation joins the fresh white vinasse by extra dry white wine vinasse weight proportion, mixes then, obtains medium matrix;
(3) the yeast-inoculated agent is joined by in the extra dry white wine vinasse weight proportion medium matrix;
(4) adjustment water content; By the above-mentioned the 1st) amount of step extra dry white wine vinasse calculates amount of water, the adjustment house water yield;
(5) initial heat insulating culture;
(6) add behind the nutrition accessory heat insulating culture again: the Ensure Liquid auxiliary material is for once adding, or the gradation adding, adds behind the auxiliary material heat insulating culture more at every turn; Add heat insulating culture a period of time behind the auxiliary material at every turn;
(7) oven dry and making finished product: the material of final heat insulating culture is dried, pulverized or granulation or film-making with conventional method, and promptly obtaining with the distillers ' grains is the Yeast protein feed finished product of base-material.
The described enzyme preparation that is used for the distillers ' grains fermentation is a kind of hydrolase preparation or two or more composite hydrolysis enzyme preparation of cellulase or hemicellulase or pectase; Said yeast-inoculated agent is to be fit to the barms of distillers ' grains fermented yeast after suitable enlarged culture; Said nutrition accessory comprises molasses and/or nitrogenous auxiliary material, and said nitrogenous auxiliary material is urea or sulfate of ammoniac or ammonium hydrogencarbonate or ammonium carbonate or ammonium phosphate, MAP, Diammonium phosphate (DAP) wherein one or both or two or more mixture.Described barms is barms that to be bacterial classification with the brewer's yeast be prepared from through one-level or secondary enlarged culture; Said distillers ' grains is the poor and/or distills ' grains of Xiaoqu wine.When enzyme preparation is used in distillers ' grains interpolation fermentation, addition is an extra dry white wine vinasse weight 0.1%～5%; Said yeast-inoculated agent adds by the amount that every gram extra dry white wine vinasse contain saccharomycete 0.1～1.0 hundred million; Said adjustment water content is to add water adjustment distillers ' grains water content to 35%～65%; The temperature of initial heat insulating culture is 28～30 ℃, and the heat insulating culture time is 30～45 hours; Said nutrition accessory addition is: the molasses addition is 4～10% of an extra dry white wine vinasse weight, and/or nitrogenous auxiliary material addition by nitrogen content count per kilogram extra dry white wine vinasse contain 0.25～0.8 the gram purity nitrogen; The heat insulating culture time is 45～55 hours behind the Ensure Liquid auxiliary material for the first time, and adding the auxiliary material heat insulating culture time for the second time is 65～75 hours, and twice holding temperature is 28～30 ℃.
By above-mentioned rule, present embodiment is described to be the Yeast protein feed production method of base-material with the distillers ' grains, and concrete steps and relevant parameter are:
(1) take by weighing distillers ' grains be fresh Xiaoqu wine poor 2000 kilograms as base-material: measuring its water content is 50%, and the weight that calculates the extra dry white wine vinasse is 1000 kilograms, and the weight by the extra dry white wine vinasse adds following materials then:
The enzyme preparation that (2) will be used for distillers ' grains fermentation by extra dry white wine vinasse weight 4%, promptly 40 kilograms, join in the fresh white vinasse, mix the acquisition medium matrix then; Said enzyme preparation is the composite hydrolysis enzyme preparation of cellulase, hemicellulase, pectase;
(3) the yeast-inoculated agent is contained 0.6 hundred million proportionings of saccharomycete, is that the access amount of yeast-inoculated agent is 60 kilograms and joins in above-mentioned (2) the step medium matrix by every gram extra dry white wine vinasse; Said yeast-inoculated agent is to be the barms that bacterial classification is prepared from through the secondary enlarged culture with the brewer's yeast;
(4) behind above-mentioned (3) EOS, press the above-mentioned the 1st) step extra dry white wine vinasse adjustment, realize that moisture distillers ' grains water content is 55%; Moisture because of the fresh white vinasse, add water only and then be 222.2 kilograms;
(5) initial heat insulating culture: under 29 ℃ of conditions, carried out initial heat insulating culture 30 hours after adjusting water content;
(6) add behind the nutrition accessory heat insulating culture again: initial heat insulating culture is accomplished the 4% adding molasses that the extra dry white wine vinasse are pressed in the back; Promptly add 40 kilograms, add the auxiliary material that contains 0.25 gram purity nitrogen by per kilogram extra dry white wine vinasse simultaneously, promptly contain the nitrogenous auxiliary material of purity nitrogen 250 grams altogether; Preferred urea, addition are 543 grams; Heat insulating culture 51 hours again after the adding adds auxiliary material more for the second time, and addition is identical with the first time; And then heat insulating culture end in 72 hours, twice holding temperature range of choice is 28～30 ℃;
(7) oven dry and making finished product: powdery is dried, is ground into to the material of final heat insulating culture by conventional method, and promptly obtaining with the distillers ' grains is the Yeast protein feed finished product of base-material; Every gram product contains saccharomycete 45～5,000,000,000.
Embodiment 4: different with the foregoing description 3 is:
Described is the Yeast protein feed production method of base-material with the distillers ' grains, and present embodiment concrete steps and relevant parameter are:
(1) take by weighing distillers ' grains, mix totally 1818 kilograms as base-material for fresh Xiaoqu wine is poor with distills ' grains: measuring its water content is 45%, and the weight that calculates the extra dry white wine vinasse is 1000 kilograms, and the weight by the extra dry white wine vinasse adds following materials then:
The enzyme preparation that (2) will be used for distillers ' grains fermentation by extra dry white wine vinasse weight 5%, promptly 50 kilograms, join in the fresh white vinasse, mix the acquisition medium matrix then; Said enzyme preparation is the cellulose hydrolyzation enzyme preparation;
(3) the yeast-inoculated agent is contained 100,000,000 proportionings of saccharomycete by every gram extra dry white wine vinasse and join in above-mentioned (2) the step medium matrix, the access amount of yeast-inoculated agent is 100 kilograms; Said yeast-inoculated agent is to be the barms that bacterial classification is prepared from through the one-level enlarged culture with the brewer's yeast;
(4) finish the back by the above-mentioned the 1st above-mentioned (3)) step extra dry white wine vinasse adjustment, moisture distillers ' grains water content is 60%, and is moisture because of the fresh white vinasse, adds water only and then is 682 kilograms;
(5) initial heat insulating culture: under 28 ℃ of conditions, carried out initial heat insulating culture 45 hours after adjusting water content;
(6) add behind the nutrition accessory heat insulating culture again: initial heat insulating culture is accomplished the 10% adding molasses that the extra dry white wine vinasse are pressed in the back; Promptly add 100 kilograms, and then heat insulating culture 55 hours hours, add nitrogenous auxiliary material at last again; Add the auxiliary material that contains 0.8 gram purity nitrogen by per kilogram extra dry white wine vinasse; The nitrogenous auxiliary material that promptly contains purity nitrogen 800 grams altogether selects ammonium sulfate to add, and amounting to addition is 3810 grams. and then heat insulating culture finished in 75 hours; Can also select urea, or ammonium hydrogencarbonate, ammonium carbonate, ammonium phosphate comprises MAP, Diammonium phosphate (DAP), perhaps they is mixed and adds; When selecting urea for use, addition is 1739 grams; Every gram product contains saccharomycete 30～3,500,000,000.
(7) oven dry and making finished product: the system minitype particle is dried, pulverized to the material of final heat insulating culture by conventional method, and promptly obtaining with the distillers ' grains is the Yeast protein feed finished product of base-material.
Embodiment 5: different with the foregoing description 3 is: described is the Yeast protein feed production method of base-material with the distillers ' grains, and its distillers ' grains fermentation uses enzyme preparation to be 4% of extra dry white wine vinasse weight; Add water adjustment distillers ' grains water content to 35%; The temperature of initial heat insulating culture is 30 ℃, and the heat insulating culture time is 45 hours; Said nutrition accessory addition is: the molasses addition is 5% of an extra dry white wine vinasse weight, and nitrogenous auxiliary material addition is counted per kilogram extra dry white wine vinasse by nitrogen content and contained 0.5 gram purity nitrogen; The every gram product of Yeast protein feed contains saccharomycete 20～3,000,000,000.
Embodiment 6: different with the foregoing description 3 is: described is the Yeast protein feed production method of base-material with the distillers ' grains, and its distillers ' grains fermentation uses enzyme preparation to be 0.1% of extra dry white wine vinasse weight; The yeast-inoculated agent contains 0.1 hundred million proportionings of saccharomycete by every gram extra dry white wine vinasse; Distillers ' grains is fresh distills ' grains; Nutrition accessory comprises molasses and nitrogenous auxiliary material, and molasses are 6% of extra dry white wine vinasse weight, and its nitrogen content of nitrogenous auxiliary material is that per kilogram extra dry white wine vinasse contain 0.3 gram purity nitrogen; The every gram product of Yeast protein feed contains saccharomycete 30～3,500,000,000.
Embodiment 7: different with the foregoing description 3 is: described interpolation nutrition accessory only adds molasses, also can only add a kind of in the nitrogenous auxiliary material.
Claims (6)
1. one kind is the Yeast protein feed production method of base-material with the distillers ' grains; It is characterized in that, be base-material with the distillers ' grains, and add the material of participating in fermented and cultured and comprise: distillers ' grains ferments with enzyme preparation, yeast-inoculated agent and nutrition accessory; Heat insulating culture after mixing, product is processed in oven dry at last; Every gram product contains saccharomycete 20～5,000,000,000; It is following that its concrete system is joined step:
(1) select distillers ' grains for use: take by weighing the fresh white vinasse as base-material, measure its water content, calculate the weight of extra dry white wine vinasse, the weight by the extra dry white wine vinasse adds following materials then:
The enzyme preparation that (2) will be used for distillers ' grains fermentation joins the fresh white vinasse by extra dry white wine vinasse weight proportion, mixes then, obtains medium matrix;
(3) the yeast-inoculated agent is joined by in the extra dry white wine vinasse weight proportion medium matrix;
(4) adjustment water content; By the above-mentioned the 1st) amount of step extra dry white wine vinasse calculates amount of water, the adjustment house water yield;
(5) initial heat insulating culture;
(6) add behind the nutrition accessory heat insulating culture again: the Ensure Liquid auxiliary material is for once adding, or the gradation adding, adds behind the auxiliary material heat insulating culture more at every turn; Add heat insulating culture a period of time behind the auxiliary material at every turn;
(7) oven dry and making finished product: the material of final heat insulating culture is dried, pulverized or granulation or film-making with conventional method, and promptly obtaining with the distillers ' grains is the Yeast protein feed finished product of base-material;
The said enzyme preparation that is used for the distillers ' grains fermentation is a kind of hydrolase preparation or two or more composite hydrolysis enzyme preparation of cellulase or hemicellulase or pectase; Said yeast-inoculated agent is to be fit to the barms of distillers ' grains fermented yeast after suitable enlarged culture; Said nutrition accessory comprises molasses and/or nitrogenous auxiliary material, and said nitrogenous auxiliary material is urea or sulfate of ammoniac or ammonium hydrogencarbonate or ammonium carbonate or ammonium phosphate, MAP, Diammonium phosphate (DAP) wherein one or both or two or more mixture;
Said distillers ' grains fermentation uses the enzyme preparation addition to be extra dry white wine vinasse weight 0.1%～5%; Said yeast-inoculated agent adds by the amount that every gram extra dry white wine vinasse contain saccharomycete 0.1～1.0 hundred million; Said adjustment water content is to add water adjustment distillers ' grains water content to 35%～65%; The temperature of initial heat insulating culture is 28～30 ℃, and the heat insulating culture time is 30～45 hours; Said nutrition accessory addition is: the molasses addition is 4～10% of an extra dry white wine vinasse weight, and/or nitrogenous auxiliary material addition by nitrogen content count per kilogram extra dry white wine vinasse contain 0.25～0.8 the gram purity nitrogen; The heat insulating culture time is 45～55 hours behind the Ensure Liquid auxiliary material for the first time, and adding the auxiliary material heat insulating culture time for the second time is 65～75 hours, and twice holding temperature is 28～30 ℃.
2. according to claim 1 is the Yeast protein feed production method of base-material with the distillers ' grains, it is characterized in that, described barms is barms that to be bacterial classification with the brewer's yeast be prepared from through one-level or secondary enlarged culture; Said distillers ' grains is the poor and/or distills ' grains of Xiaoqu wine.
3. according to claim 1 and 2 is the Yeast protein feed production method of base-material with the distillers ' grains, is characterised in that altogether step is:
(1) take by weighing distillers ' grains be fresh Xiaoqu wine poor 2000 kilograms as base-material: measuring its water content is 50%, and the weight that calculates the extra dry white wine vinasse is 1000 kilograms, and the weight by the extra dry white wine vinasse adds following materials then:
The enzyme preparation that (2) will be used for distillers ' grains fermentation by extra dry white wine vinasse weight 4%, promptly 40 kilograms, join in the fresh white vinasse, mix the acquisition medium matrix then; Said enzyme preparation is the composite hydrolysis enzyme preparation of cellulase, hemicellulase, pectase;
(3) the yeast-inoculated agent is contained 0.6 hundred million proportionings of saccharomycete, is that the access amount of yeast-inoculated agent is 60 kilograms and joins in above-mentioned (2) the step medium matrix by every gram extra dry white wine vinasse; Said yeast-inoculated agent is to be the barms that bacterial classification is prepared from through the secondary enlarged culture with the brewer's yeast;
(4) behind above-mentioned (3) EOS, press the above-mentioned the 1st) step extra dry white wine vinasse adjustment, realize that moisture distillers ' grains water content is 55%; Moisture because of the fresh white vinasse, add water only and then be 222.2 kilograms;
(5) initial heat insulating culture: under 29 ℃ of conditions, carried out initial heat insulating culture 30 hours after adjusting water content;
(6) add behind the nutrition accessory heat insulating culture again: initial heat insulating culture is accomplished the 4% adding molasses that the extra dry white wine vinasse are pressed in the back; Promptly add 40 kilograms; Add the auxiliary material that contains 0.25 gram purity nitrogen by per kilogram extra dry white wine vinasse simultaneously, promptly contain the nitrogenous auxiliary material urea of purity nitrogen 250 grams altogether, addition is 543 grams; Heat insulating culture 51 hours again after the adding adds auxiliary material more for the second time, and addition is identical with the first time; And then heat insulating culture end in 72 hours, twice holding temperature range of choice is 28～30 ℃;
(7) oven dry and making finished product: powdery is dried, is ground into to the material of final heat insulating culture by conventional method, and promptly obtaining with the distillers ' grains is the Yeast protein feed finished product of base-material; Every gram product contains saccharomycete 45～5,000,000,000.
4. according to claim 1 and 2 is the Yeast protein feed production method of base-material with the distillers ' grains, it is characterized in that step is:
(1) take by weighing distillers ' grains, mix totally 1818 kilograms as base-material for fresh Xiaoqu wine is poor with distills ' grains: measuring its water content is 45%, and the weight that calculates the extra dry white wine vinasse is 1000 kilograms, and the weight by the extra dry white wine vinasse adds following materials then:
The enzyme preparation that (2) will be used for distillers ' grains fermentation by extra dry white wine vinasse weight 5%, promptly 50 kilograms, join in the fresh white vinasse, mix the acquisition medium matrix then; Said enzyme preparation is the cellulose hydrolyzation enzyme preparation;
(3) the yeast-inoculated agent is contained 100,000,000 proportionings of saccharomycete by every gram extra dry white wine vinasse and join in above-mentioned (2) the step medium matrix, the access amount of yeast-inoculated agent is 100 kilograms; Said yeast-inoculated agent is to be the barms that bacterial classification is prepared from through the one-level enlarged culture with the brewer's yeast;
(4) finish the back by the above-mentioned the 1st above-mentioned (3)) step extra dry white wine vinasse adjustment, moisture distillers ' grains water content is 60%, and is moisture because of the fresh white vinasse, adds water only and then is 682 kilograms;
(5) initial heat insulating culture: under 28 ℃ of conditions, carried out initial heat insulating culture 45 hours after adjusting water content;
(6) add behind the nutrition accessory heat insulating culture again: initial heat insulating culture is accomplished the 10% adding molasses that the extra dry white wine vinasse are pressed in the back; Promptly add 100 kilograms, and then heat insulating culture 55 hours, add nitrogenous auxiliary material at last again; Add the auxiliary material that contains 0.8 gram purity nitrogen by per kilogram extra dry white wine vinasse; The nitrogenous auxiliary material that promptly contains purity nitrogen 800 grams altogether selects ammonium sulfate to add, and amounting to addition is 3810 grams. and then heat insulating culture finished in 75 hours; Or select urea, or ammonium hydrogencarbonate, ammonium carbonate, ammonium phosphate, MAP, Diammonium phosphate (DAP) is perhaps mixed them and is added; When selecting urea for use, addition is 1739 grams; Every gram product contains saccharomycete 30～3,500,000,000;
(7) oven dry and making finished product: the system minitype particle is dried, pulverized to the material of final heat insulating culture by conventional method, and promptly obtaining with the distillers ' grains is the Yeast protein feed finished product of base-material.
5. according to claim 1 is the Yeast protein feed production method of base-material with the distillers ' grains, it is characterized in that, the distillers ' grains fermentation uses enzyme preparation to be 4% of extra dry white wine vinasse weight; Add water adjustment distillers ' grains water content to 35%; The temperature of initial heat insulating culture is 30 ℃, and the heat insulating culture time is 45 hours; Said nutrition accessory addition is: the molasses addition is 5% of an extra dry white wine vinasse weight, and nitrogenous auxiliary material addition is counted per kilogram extra dry white wine vinasse by nitrogen content and contained 0.5 gram purity nitrogen; The every gram product of Yeast protein feed contains saccharomycete 20～3,000,000,000.
6. according to claim 1 is the Yeast protein feed production method of base-material with the distillers ' grains, it is characterized in that, the distillers ' grains fermentation uses enzyme preparation to be 0.1% of extra dry white wine vinasse weight; The yeast-inoculated agent contains 0.1 hundred million proportionings of saccharomycete by every gram extra dry white wine vinasse; Distillers ' grains is fresh distills ' grains; Nutrition accessory comprises molasses and nitrogenous auxiliary material, and molasses are 6% of extra dry white wine vinasse weight, and its nitrogen content of nitrogenous auxiliary material is that per kilogram extra dry white wine vinasse contain 0.3 gram purity nitrogen; The every gram product of Yeast protein feed contains saccharomycete 30～3,500,000,000.
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