CN103243053B - Fermentative bacteria solution for peanut vines and peanut vine fermentation feed - Google Patents
Fermentative bacteria solution for peanut vines and peanut vine fermentation feed Download PDFInfo
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- CN103243053B CN103243053B CN201310193653.1A CN201310193653A CN103243053B CN 103243053 B CN103243053 B CN 103243053B CN 201310193653 A CN201310193653 A CN 201310193653A CN 103243053 B CN103243053 B CN 103243053B
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Abstract
The invention discloses a fermentative bacteria solution for peanut vines and a peanut vine fermentation feed, which belong to the technical field of fermentation feeds. The fermentative bacteria solution for peanut vines is composed of the following components in parts by weight: 30-40 parts of a bacteria solution A and 10-15 parts of a bacteria solution B, every 40g of the bacteria solution A contains cellulomonas (1*10<9>-4*10<9> CFU), butyrivibrio fibrisolvens (1.5*10<9>-5*10<9> CFU), saccharomyces cerevisiae (1.68*10<9>-4.32*10<9> CFU), bacillus subtilis (2.25*10<10>-6.67*10<10> CFU), corn steep liquor (1-3g by dry matters), and the balance of water and necessary microbial matrixes; and every 10g of bacteria solution B contains lactic acid bacteria (0.22*10<10>-0.96*10<10> CFU), corn steep liquor (0.47-1.35g by dry matters), whole milk powder (0.67-2g), and the balance of water and necessary microbial matrixes. A peanut vine fermentation feed prepared by using the fermentative bacteria solution is prepared from the following components in parts by weight through mixing and fermenting: 45-55 parts of peanut vine powder, 5-8 parts of corn flour, 2-3 parts of soybean meal powder, 1-2 parts of urea, 1-2 parts of calcium sulphate and 40-55 parts of the fermentative bacteria solution.
Description
Technical field
The invention belongs to fermented feed technical field, the fermented feed that relates to a kind of zymocyte liquid and utilize this zymocyte liquid to make.
Background technology
Peanut seedling quality is soft and nutritive substance abundant, the equal edible of various livestock and poultry.Relevant Analysis measurement result shows, in the peanut seedling cauline leaf of sprawl growth, contain crude protein approximately 12.00%～13.50%, crude fat approximately 2.00%～3.00%, carbohydrate (being mainly robust fibre) approximately 46.00%～48%, wherein the gross protein value of Folium Arachidis hypogaeae is up to 20%.With regard to digestible protein, 1 kilogram of dry peanut seedling, approximately containing 70 grams of digestible proteins, contains 17 grams of calcium, 7 grams of phosphorus in addition.The gross protein value of peanut seedling is 1.6 times of pea seedling, is 6 times of straw.The livestock and poultry energy that energy equivalence that 1 kilogram of peanut seedling produces produces in 0.6 kilogram of barley of searching for food.300 kilograms of peanuts of general every mu of real estate just can obtain the peanut seedling of 300 kilograms, are equivalent to the feeding effect of 180 kilograms of barleys for the domestic animal of feeding.
Peanut seedling is inexpensive, but current utilization rate is lower, and its processing mode is still to burn also Tian Weizhu.Trace it to its cause, be mainly because also there is following defect as feed peanut seedling: 1) crude fiber content is high, and livestock and poultry digestibility is low; 2) not resistance to storage: peanut seedling stacking floor space is large, and be easily subject to miscellaneous bacteria affect and go mouldy.
Summary of the invention
The object of the invention is to provides a kind of pair of component zymocyte liquid for peanut seedling, and the peanut seedling fermented feed that utilizes this zymocyte liquid to make is also provided simultaneously.
Based on above-mentioned purpose, the present invention has taked following technical scheme:
Peanut seedling zymocyte liquid, is made up of the A bacterium liquid of 30～40 weight parts and the B bacterium liquid of 10～15 weight parts, wherein fibre-bearing Zymomonas mobilis 1 × 10 in every 40g A bacterium liquid
9～4 × 10
9cFU, Butyrivibrio fibrisolvens 1.5 × 10
9～5 × 10
9cFU, S. cervisiae 1.68 × 10
9～4.32 × 10
9cFU, subtilis 2.25 × 10
10～6.67 × 10
10cFU, corn steep liquor are in dry-matter 1～3g, and all the other are water and necessary microorganism matrix (contained microorganism matrix in commercial bacterium powder); In every 10g B component, contain milk-acid bacteria 0.22 × 10
10～0.96 × 10
10cFU, corn steep liquor is in dry-matter 0.47-1.35g, whole milk powder 0.67～2g, and all the other are water and necessary microorganism matrix.
Get cellulomonas cartae preparation, Butyrivibrio fibrisolvens preparation, yeast saccharomyces cerevisiae bacteria preparation, bacillus subtilis formulation, corn steep liquor and water and mix, then within 21～27 hours, obtain A bacterium liquid in 25～28 DEG C of airtight placements.
Extracting lactic acid bacteria preparation, corn steep liquor, whole milk powder and water mix, and then within 42～54 hours, obtain B bacterium liquid in 25～28 DEG C of airtight placements.
The peanut seedling fermented feed that utilizes described zymocyte liquid to make, in weight part, this fermented feed is formed by 45～55 parts of peanut vine powders, 5～8 portions of Semen Maydis powder, 2～3 parts of bean cake powders, 1～2 part of urea, 1～2 part of calcium sulfate and 40～55 parts of zymocyte liquid mixing, fermentations.
Described peanut vine powder, Semen Maydis powder, bean cake powder are crossed 1.5～2.5 mm sieve.
Described fermentation is carried out in the fermentation bag with one-way exhaust valve, and 25～28 DEG C of leavening temperatures treat that in fermentation bag, mixture air-blowing can stop fermentation.
Crude protein and the crude fat content that can improve peanut seedling with zymocyte liquid provided by the invention, reduces crude fiber content, the immunity function of generation raising simultaneously
linear gramicidinswith the multiple active ingredient that contributes to digestion, as vitamin B group, digestive ferment, cellulase etc.Wherein, cellulomonas cartae (Cellulomonas) can be at decomposition of cellulose under aerobic condition, and the anerobe as Butyrivibrio fibrisolvens (Butyrivibrio fibrisolvens) so can be decomposed Mierocrystalline cellulose at anaerobic condition.S. cervisiae can suck the monose such as glucose, fructose, seminose in cell, under oxygen free condition, through the effect of endoenzyme, monose fermentation is decomposed into carbonic acid gas and alcohol.Yeast rich vitamin B, protein and plurality of enzymes, contribute to digesting and assimilating of nutrient.Subtilis
thallinethe subtilyne that produces in process of growth, polymyxin, nystatin,
linear gramicidinsisoreactivity material to pathogenic bacterium or
autogenous infectionconditioned pathogen have obvious restraining effect.Meanwhile, subtilis also can consume rapidly the free oxygen in environment, further promotes useful
anerobegrowth, and produce lactic acid etc.
organic acidclass, reduces enteron aisle pH value, indirectly suppresses other pathogenic bacterium growth.Subtilis thalline self synthesize α-amylase,
proteolytic enzyme, lipase,
mierocrystalline cellulosethe enzymes such as enzyme, can synthesize
vitamins B 1 , B
2, B
6, the multiple vitamin B group such as nicotinic acid, and improve in animal body
interferon, rabbitactivity with scavenger cell.Supplement the effect of some milk-acid bacterias
Peanut seedling fermented feed provided by the invention, taking peanut seedling as core material, is aided with Semen Maydis powder, bean cake powder, urea and calcium sulfate, after inoculation fermentation liquid, can ferment, and conventionally after 24～48 hours, just can obtain ideal product.Test-results shows, in this fermented feed, crude fiber content reduces, histone matter content improves, the palatability of feed is improved, in middle hog grower feed, add 5～10% the alternative equivalent wheat bran of peanut seedling fermented feed, can promote pork pig to grow, reduce pork pig aquaculture cost, there is good market popularization value.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
embodiment 1
Peanut seedling zymocyte liquid, is made up of 30 kgA bacterium liquid and 10kg B bacterium liquid.
Wherein fibre-bearing Zymomonas mobilis 4 × 10 in every 40g A bacterium liquid
9cFU, Butyrivibrio fibrisolvens 5 × 10
9cFU, S. cervisiae 4.32 × 10
9cFU, subtilis 6.67 × 10
10cFU, corn steep liquor are in dry-matter 3g, and all the other are water and necessary microorganism matrix.Get 1.0 kg cellulomonas cartae solid preparation (viable count content 3.0 × 10
9cFU/g), 2.5 kg Butyrivibrio fibrisolvens solid preparation (viable count content 1.5 × 10
9cFU/g), 1.8 kg S. cervisiae solid preparation (viable count content 1.8 × 10
9cFU/g), 2.0 kg subtilis solid preparation (viable count content 2.5 × 10
10cFU/g), 5kg corn steep liquor (dry matter content 45wt%) mix, add water and supply 30kg, then within 21 hours, obtain A bacterium liquid in 25～28 DEG C of airtight placements.
In every 10g B component, contain milk-acid bacteria 0.96 × 10
10cFU, corn steep liquor is in dry-matter 1.35g, whole milk powder 2g, and all the other are water and necessary microorganism matrix.Get 0.6kg milk-acid bacteria solid preparation (viable count content 1.6 × 10
10cFU/g), 3kg corn steep liquor (dry matter content 45wt%), 2kg whole milk powder mix, and adds water and supply 10kg, after mixing, within 42 hours, obtains B bacterium liquid in 25～28 DEG C of airtight placements.
Peanut seedling fermented feed, is formed by 45kg peanut vine powder, 5 kg Semen Maydis powder, 2kg bean cake powder, 1 kg urea, 1 kg calcium sulfate and the mixing of 40 kg zymocyte liquids, fermentation; Peanut vine powder, Semen Maydis powder, bean cake powder are crossed 1.5 mm sieve; Fermenting process carries out in the fermentation bag with one-way exhaust valve, and 25 DEG C of leavening temperatures treat that in fermentation bag, mixture air-blowing can stop fermentation.
embodiment 2
Peanut seedling zymocyte liquid, is made up of 40 kg A bacterium liquid and 15 kg B bacterium liquid.
Wherein fibre-bearing Zymomonas mobilis 1 × 10 in every 40g A bacterium liquid
9cFU, Butyrivibrio fibrisolvens 1.5 × 10
9cFU, S. cervisiae 1.68 × 10
9cFU, subtilis 2.25 × 10
10cFU, corn steep liquor are in dry-matter 1 g, and all the other are water and necessary microorganism matrix.Get 0.5kg cellulomonas cartae solid preparation (viable count content 2.0 × 10
9cFU/g), 1.5 kg Butyrivibrio fibrisolvens solid preparation (viable count content 1.0 × 10
9cFU/g), 1.2kg S. cervisiae solid preparation (viable count content 1.4 × 10
9cFU/g), 1.5kg subtilis solid preparation (viable count content 1.5 × 10
10cFU/g), 3kg corn steep liquor (dry matter content 34wt%), mix and add water and supply 40kg, after mixing, within 27 hours, obtain A bacterium liquid in 25～28 DEG C of airtight placements.
In every 10g B component, contain milk-acid bacteria 0.22 × 10
10cFU, corn steep liquor is in dry-matter 0.47g, whole milk powder 0.67g, and all the other are water and necessary microorganism matrix.Take 0.3kg lactic acid bacteria formulation (viable count content 1.1 × 10
10cFU/g), 2kg corn steep liquor (dry matter content 35wt%), 1kg whole milk powder mix, and adds water and supply 15kg, after mixing, within 54 hours, obtains B bacterium liquid in 25～28 DEG C of airtight placements.
Peanut seedling fermented feed, is formed by 55 kg peanut vine powders, 8 kg Semen Maydis powder, 3 kg bean cake powders, 2 kg urea, 2 kg calcium sulfate and the mixing of 55 kg zymocyte liquids, fermentation; Peanut vine powder, Semen Maydis powder, bean cake powder are crossed 2.5 mm sieve; Fermenting process carries out in the fermentation bag with one-way exhaust valve, and 28 DEG C of leavening temperatures treat that in fermentation bag, mixture air-blowing can stop fermentation.
embodiment 3
Peanut seedling zymocyte liquid, is made up of 35 kg A bacterium liquid and 12 kg B bacterium liquid.
Wherein fibre-bearing Zymomonas mobilis 2 × 10 in every 40g A bacterium liquid
9cFU, Butyrivibrio fibrisolvens 3 × 10
9cFU, S. cervisiae 3 × 10
9cFU, subtilis 4 × 10
10cFU, corn steep liquor are in dry-matter 2g, and all the other are water and necessary microorganism matrix.Take in proportion cellulomonas cartae solid preparation (2.5 × 10
9cFU/g), Butyrivibrio fibrisolvens solid preparation (1.2 × 10
9cFU/g), S. cervisiae solid preparation (1.6 × 10
9cFU/g), subtilis solid preparation (2.0 × 10
10cFU/g), corn steep liquor (dry matter content 40wt%) mix, add water and supply 35kg, after mixing, within 24 hours, obtain A bacterium liquid in 25～28 DEG C of airtight placements.
In every 10g B component, contain milk-acid bacteria 0.5 × 10
10cFU, corn steep liquor is in dry-matter 0.8g, whole milk powder 1.5g, and all the other are water and necessary microorganism matrix.Take in proportion milk-acid bacteria solid preparation (1.2 × 10
10cFU/g), corn steep liquor (dry matter content 40wt%), whole milk powder mix, and adds water and supply 12kg, after mixing, within 48 hours, obtains B bacterium liquid in 25～28 DEG C of airtight placements.
Peanut seedling fermented feed, is formed by 50 kg peanut vine powders, 6 kg Semen Maydis powder, 2.5 kg bean cake powders, 1.6 kg urea, 1.5 kg calcium sulfate and the mixing of 47 kg zymocyte liquids, fermentation; Peanut vine powder, Semen Maydis powder, bean cake powder are crossed 2.0 mm sieve; Fermenting process carries out in the fermentation bag with one-way exhaust valve, and 26 DEG C of leavening temperatures treat that in fermentation bag, mixture air-blowing can stop fermentation.
embodiment 4
Press the Feed Sample method of sampling, not 6 parts of fermenting peanut seedling mixture and fermenting peanut seedling blend sample of random acquisition from embodiment 1-3 respectively, according to crude protein, crude fat, crude fiber content in feed conventional nutrient analytical procedure working sample, measurement result is in table 1.As can be seen from Table 1, after peanut seedling fermented mixture, improve crude protein and crude fat content, reduced crude fiber content.
The variation of component content before and after table 1 peanut seedling fermented mixture
| ? | Crude protein | Crude fat | Robust fibre |
| Not fermenting peanut seedling mixture % | 12.62±1.02 a | 2.21±0.12 a | 46.52±4.25 a |
| Fermenting peanut seedling mixture % | 15.81±1.14 b | 2.84±0.11 b | 40.22±4.12 b |
| Rangeability | +25.28% | +28.51% | -13.54% |
Note: the identical English alphabet of same column represents that significantly (P >=0.05), different English alphabet do not represent significant difference (P≤0.05) to difference.
embodiment 5 feeding effect tests
5.1 test method
Select 65 ages in days, mean body weight 25.12kg(25.12 ± 0.50kg) 540 of DLY three way cross growing swine; Test pig is divided into 9 treatment group, 6 repetitions of each treatment group, 10 of each repetitions according to the each group of approaching principle of male and female number.9 treatment group are designated as respectively 1 group of contrast, 2 groups of contrasts, 3 groups of contrasts, test 1-6 group.Each group pig daily ration proportioning is referring to table 2, and day feeds three times, freely drinks water.Test period is 90 days.
The each test group pig of table 2 daily ration proportioning (weight ratio adds up to 1000)
| Numbering | Corn | Dregs of beans | Wheat bran | Fish meal | Not fermenting peanut seedling mixture | Fermenting peanut seedling mixture |
| Contrast 1 group | 630 | 240 | 110 | 20 | - | - |
| Contrast 2 groups | 630 | 240 | 50 | 20 | 60 | - |
| Contrast 3 groups | 630 | 240 | 30 | 20 | 80 | - |
| Test 1 group | 630 | 240 | 50 | 20 | - | 60 parts of embodiment 1 products |
| Test 2 groups | 630 | 240 | 30 | 20 | - | 80 parts of embodiment 1 products |
| Test 3 groups | 630 | 240 | 50 | 20 | - | 60 parts of embodiment 2 products |
| Test 4 groups | 630 | 240 | 30 | 20 | - | 80 parts of embodiment 2 products |
| Test 5 groups | 630 | 240 | 50 | 20 | - | 60 parts of embodiment 3 products |
| Test 6 groups | 630 | 240 | 30 | 20 | - | 80 parts of embodiment 3 products |
5.2 test-results
Can be found out by table 3, table 4, table 5 result, and contrast 1 group and compare, in daily ration, add after 6%, 8% not fermenting peanut seedling mixture replacing wheat bran, reduce day weight gain and the food consumption of growing swine fattening pig, improve feedstuff-meat ratio; With contrast 1 group and compare with fermenting peanut seedling mixture group not, in daily ration, add after 6%, 8% the fermenting peanut seedling mixture replacing wheat bran of embodiment 1,2,3, obviously improve day weight gain and the food consumption of growing swine fattening pig, reduce feedstuff-meat ratio.
The impact (test period 1-45d) of table 3 fermenting peanut seedling on store pig production performance
The impact (test period 46-90d) of table 4 fermenting peanut seedling on store pig production performance
The impact (test period 1-90d) of table 5 fermenting peanut seedling on store pig production performance
5.3 interpretation of result
Found out by table 6, table 7 interpretation of result, and contrast 1 group and compare, in daily ration, add after 6%, 8% not fermenting peanut seedling mixture replacing wheat bran, obviously reduce day weight gain and the food consumption in each stage of growing swine fattening pig, improve feedstuff-meat ratio; With contrast 1 group and compare with fermenting peanut seedling mixture group not, in daily ration, add after 6%, 8% the fermenting peanut seedling mixture replacing wheat bran of embodiment 1,2,3, obviously improve day weight gain and the food consumption in each stage of growing swine fattening pig, reduce feedstuff-meat ratio.Test-results shows: it is production performance that fermenting peanut seedling mixture can obviously improve store pig, can significantly promote store pig growth and feed conversion, improves day weight gain, reduces feedstuff-meat ratio.
Table 6 test group is compared % with not substituting wheat bran (contrasting 1 group)
Table 7 test group with add not the control group of fermenting peanut seedling mixture (mean value) % that compares
Claims (4)
1. peanut seedling zymocyte liquid, is made up of the A bacterium liquid of 30～40 weight parts and the B bacterium liquid of 10～15 weight parts, wherein fibre-bearing Zymomonas mobilis 1 × 10 in every 40g A bacterium liquid
9～4 × 10
9cFU, Butyrivibrio fibrisolvens 1.5 × 10
9～5 × 10
9cFU, S. cervisiae 1.68 × 10
9～4.32 × 10
9cFU, subtilis 2.25 × 10
10～6.67 × 10
10cFU, corn steep liquor are in dry-matter 1～3g, and all the other are water and necessary microorganism matrix; In every 10g B component, contain milk-acid bacteria 0.22 × 10
10～0.96 × 10
10cFU, corn steep liquor is in dry-matter 0.47-1.35g, whole milk powder 0.67～2g, and all the other are water and necessary microorganism matrix; Get cellulomonas cartae preparation, Butyrivibrio fibrisolvens preparation, yeast saccharomyces cerevisiae bacteria preparation, bacillus subtilis formulation, corn steep liquor and water and mix, then within 21～27 hours, obtain A bacterium liquid in 25～28 DEG C of airtight placements; Extracting lactic acid bacteria preparation, corn steep liquor, whole milk powder and water mix, and then within 42～54 hours, obtain B bacterium liquid in 25～28 DEG C of airtight placements.
2. utilize claim 1 to state the peanut seedling fermented feed that zymocyte liquid is made, it is characterized in that, in weight part, described fermented feed is formed by 45～55 parts of peanut vine powders, 5～8 portions of Semen Maydis powder, 2～3 parts of bean cake powders, 1～2 part of urea, 1～2 part of calcium sulfate and 40～55 parts of zymocyte liquid mixing, fermentations.
3. peanut seedling fermented feed as claimed in claim 2, is characterized in that, described peanut vine powder, Semen Maydis powder, bean cake powder are crossed 1.5～2.5 mm sieve.
4. peanut seedling fermented feed as claimed in claim 3, is characterized in that, described fermentation is carried out in the fermentation bag with one-way exhaust valve, and 25～28 DEG C of leavening temperatures treat that in fermentation bag, mixture air-blowing can stop fermentation.
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| CN105076892A (en) * | 2015-09-23 | 2015-11-25 | 安徽省兴源草业有限公司 | Sweet potato vine fermented feed for sows |
| CN107494919A (en) * | 2017-10-11 | 2017-12-22 | 安徽省宿州市民辉养殖有限公司 | Big pig's feed and preparation method thereof in one kind |
| CN107712271A (en) * | 2017-10-23 | 2018-02-23 | 江苏棉海园林工程有限公司 | A kind of preparation method and its leavening of fermenting peanut seedling |
| CN110338269A (en) * | 2019-08-09 | 2019-10-18 | 刘建军 | A method of nonreactive mutton sheep TMR particulate material is produced using peanut vine |
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| CN1194624C (en) * | 2003-01-20 | 2005-03-30 | 丁友昉 | Multiple microorganism straw fermented fodder and leaven and its preparing method |
| CN1295981C (en) * | 2004-11-26 | 2007-01-24 | 天津科技大学 | Microbial fermented feed, microbe mother liquor for fermented feed and its production process |
| CN101401616B (en) * | 2008-11-12 | 2012-09-19 | 北京阔利达生物技术开发有限公司 | Fermented abandoned vegetable feedstuff and preparation method thereof |
| CN102308932A (en) * | 2011-03-29 | 2012-01-11 | 李华玉 | Method for preparing yellow corn silage feed from probiotics |
| CN102488084B (en) * | 2011-12-10 | 2013-04-17 | 甘肃鑫盛源牧业科技有限公司 | Method for preparing protein feed by fermentation on straw and cake using aerobic mixed bacteria |
| CN102676426A (en) * | 2012-05-09 | 2012-09-19 | 湖北省农业科学院农产品加工与核农技术研究所 | Compound bacterium agent capable of decomposing cotton stalks quickly and preparation method thereof |
| CN102805204A (en) * | 2012-07-26 | 2012-12-05 | 周建设 | Biological fermentation forage grass containing Chinese herbal medicines and used for raising pigs |
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Inventor after: Zhao Shuqiang Inventor after: Chen Wen Inventor after: Wang Mingcheng Inventor after: Niu Lian Inventor after: Dong Xiaoli Inventor after: He Tao Inventor before: He Tao Inventor before: Chen Wen Inventor before: Zhao Shuqiang Inventor before: Niu Lian Inventor before: Dong Xiaoli |
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