CN103181283A

CN103181283A - Method for acclimatizing cypripedium farreri

Info

Publication number: CN103181283A
Application number: CN 201110461008
Authority: CN
Inventors: 何聪
Original assignee: Individual
Current assignee: Individual
Priority date: 2011-12-31
Filing date: 2011-12-31
Publication date: 2013-07-03

Abstract

The invention relates to a method for acclimatizing cypripedium farreri. The wild plants of the cypripedium farreri are subjected to plant division and stem pressing in a greenhouse; an original growth environment of the cypripedium farreri is simulated through strict environment regulation, so that the survival rate is increased; and cypripedium farreri seeds with stable and excellent characteristics are obtained through continuous plant division and screening. Therefore, the method is a culture method which achieves a long-lasting effect through a one-step operation.

Description

The domesticating method of a kind of West China lady's slipper

Technical field

The invention belongs to wild plant culture technique field, relate to the domesticating method of a kind of West China lady's slipper particularly.

Background technology

High 20-30 centimetre of West China lady's slipper plant, the root-like stock that tool is sturdy and short.Stem is upright, nearly nothing hair, and base portion tool number piece sheath, the sheath top has 2 pieces of leaves usually.Blade ellipse or ovum shape ellipse, long 6-9 centimetre, wide 2.5-3.5 centimetre, the anxious point of tip or short gradually point, the two sides does not have hair, and the edge is the thin echinid of tool slightly.Produce Yunnan Zhong Dian; Be born under the sparse woods of 3400 meters of height above sea level in many stone thick grass, mainly be distributed in Sichuan, SOUTH OF GANSU.

West China lady's slipper overground part cauline leaf can be used as medicine the effect that tool dispels the wind, detoxifies, invigorates blood circulation.In the past few years, ecological degradation, West China lady's slipper quantity is also in quick minimizing, and for saving precious wild kind, the domestication becomes the most feasible method.

Summary of the invention

The objective of the invention is to provides the domesticating method of a kind of West China lady's slipper at an above-mentioned difficult problem, at the growing environment of internal mold pseudo-wild West China, greenhouse lady's slipper, successfully obtain out the domestication after, the seed of the West China lady's slipper that can not morph.The present invention is finished by following steps:

A, cultivation maternal plant: at the beginning of April, the West China lady's slipper of seeking is carefully protected, observed 3-5 days, find that the plant normal growth is vigorous, do not have pathology or death, select 100 strain West China lady's slipper band soil muds to the accurate modern greenhouse of control, band soil one wants many, can be with a small amount of accompanying plant; The rate of sheltering from heat or light reaches 50-60%, in time waters, and air humidity reaches 65-75%, and lady's slipper other trench digging in every strain West China is executed the acidic fertilizier ammonium dihydrogen phosphate (ADP) of 1.5-2Kg, keeps P in soil H between 5.5-6.2, grows continuously 12-15 days, in time the removing of dead, pathology.

B, keep down the vines of a creeping plant: the West China lady's slipper of surviving in the above-mentioned steps is carried out plant division handle, with the other loosing soil of plant, execute the chicken manure 4-5Kg post-trenching that becomes thoroughly decomposed, disposable filling is permeable; Second day, side ridging 4-5cm in the ditch was thick to eugonic branch, notes being sure not to break branch, with 200-500 times of liquid pouring back normal management of 0.1-1.0% zeatin.

C, final singling in time: after the generation secondary root of keeping down the vines of a creeping plant, weak seedling is extractd in thinning in time, and excessive growth seedling and diseased plant stay robust growth, anosis plant, in the other ridging all around of strain, impels its upwards growth, waters.

D, greenhouse management: begin normal management after the final singling, the greenhouse rate of sheltering from heat or light remains on 50-55%, dark processing 6 hours, all the other full exposures; Temperature is 20-23 ℃ of daytime, remains on 16-18 ℃ evening; Keep down the vines of a creeping plant just to begin to reduce and water, air humidity is that 50-60% get final product, produces behind the new root suitably increase irrigation volume; Topdress in time, used 1000-1500 potassium dihydrogen phosphate aqueous solution doubly, and applied with water in every 7-10 days; The dressing time that staggers sprays how clever bacterium or the tpn of 10-20%, pre-preventing disease and pest.

E, plant division is cultivated again: treat after the West China lady's slipper florescence, use that above-mentioned B, C, three steps of D are kept down the vines of a creeping plant, final singling and management, turn out the West China lady's slipper of more stalwartness.

F, obtain domestication back seed: through the continuous plant division 3-4 of above-mentioned steps generation, it is stable to choose proterties, the plant of robust growth is as gathering object, treat the seed of gathering after the maturation, be placed on after drying in the environment that close drying ventilates and preserve, wait until that the March in the coming year is used as seed and broadcasts sowing the land for growing field crops, maternal plant is continued to employ; All the other do not choose plant to excavate, and dry the back and handle as medicinal material.

The present invention be in the greenhouse to the West China lady's slipper wild plant plant division keep down the vines of a creeping plant, by environment conditioning strictly, simulate its original growing environment, improved survival rate, and through continuous plant division screening, obtaining proterties and stablize good West China lady's slipper seed, is that a kind of once-through operation exchanges permanent cultural method for.

Embodiment

Example 1, cultivation place are the Longnan City, Gansu Province

At the beginning of April, the West China lady's slipper of seeking is carefully protected, observed 3 days, find that the plant normal growth is vigorous, there are not pathology or death, select 100 strain West China lady's slipper band soil muds to the accurate modern greenhouse of control, band soil one wants many, can be with a small amount of accompanying plant; The rate of sheltering from heat or light reaches 55%, in time waters, and air humidity reaches 65%, and lady's slipper other trench digging in every strain West China is executed the acidic fertilizier diammonium phosphate of 1.5Kg, keeps P in soil H 5.8, grows continuously 12 days, in time the removing of dead, pathology.The West China lady's slipper of surviving is carried out plant division handle, with the other loosing soil of plant, execute the chicken manure 4Kg post-trenching that becomes thoroughly decomposed, disposable filling is permeable; Second day, side ridging 4cm in the ditch was thick to eugonic branch, is sure not to break branch, watered with 200 times of liquid of 0.5% zeatin.Keep down the vines of a creeping plant after the generation secondary root, weak seedling is extractd in thinning in time, and excessive growth seedling and diseased plant stay robust growth, anosis plant, in the other ridging all around of strain, impels its upwards growth, waters.Begin normal management after the final singling, the greenhouse rate of sheltering from heat or light remains on 50%, dark processing 6 hours, all the other full exposures; Temperature is 21 ℃ of daytimes, remains on 16 ℃ evening; Keep down the vines of a creeping plant just to begin to reduce and water, air humidity is 50% get final product, produces behind the new root suitably increase irrigation volume; Topdress in time, used 1000 times potassium dihydrogen phosphate aqueous solution, and applied with water in per 7 days; The dressing time that staggers sprays 15% how clever bacterium or tpn, pre-preventing disease and pest.

Treat after the West China lady's slipper florescence, to the West China lady's slipper keep down the vines of a creeping plant again, final singling and management, turn out the West China lady's slipper of more stalwartness.Cultivate 3-4 generation through continuous plant division, it is stable to choose proterties, and the plant of robust growth is as gathering object, treats the seed of gathering after the maturation, is placed on after drying in the environment that close drying ventilates to preserve, and waits until that the March in the coming year is used as seed and broadcasts sowing the land for growing field crops, and maternal plant is continued to employ; All the other do not choose plant to excavate, and dry the back and handle as medicinal material.

Example 2, cultivation place are the Longnan City, Gansu Province

At the beginning of April, the West China lady's slipper of seeking is carefully protected, observed 5 days, find that the plant normal growth is vigorous, there are not pathology or death, select 100 strain West China lady's slipper band soil muds to the accurate modern greenhouse of control, band soil one wants many, can be with a small amount of accompanying plant; The rate of sheltering from heat or light reaches 60%, in time waters, and air humidity reaches 75%, and lady's slipper other trench digging in every strain West China is executed the acidic fertilizier diammonium phosphate of 2Kg, keeps P in soil H 6.0, grows continuously 15 days, in time the removing of dead, pathology.The West China lady's slipper of surviving is carried out plant division handle, with the other loosing soil of plant, execute the chicken manure 5Kg post-trenching that becomes thoroughly decomposed, disposable filling is permeable; Second day, side ridging 5cm in the ditch was thick to eugonic branch, is sure not to break branch, watered with 500 times of liquid of 1.0% zeatin.Keep down the vines of a creeping plant after the generation secondary root, weak seedling is extractd in thinning in time, and excessive growth seedling and diseased plant stay robust growth, anosis plant, in the other ridging all around of strain, impels its upwards growth, waters.Begin normal management after the final singling, the greenhouse rate of sheltering from heat or light remains on 55%, dark processing 6 hours, all the other full exposures; Temperature is 23 ℃ of daytimes, remains on 18 ℃ evening; Keep down the vines of a creeping plant just to begin to reduce and water, air humidity is 60% get final product, produces behind the new root suitably increase irrigation volume; Topdress in time, used 1500 times potassium dihydrogen phosphate aqueous solution, and applied with water in per 10 days; The dressing time that staggers sprays how clever bacterium or the tpn of 10-20%, pre-preventing disease and pest.

Claims

1. the domesticating method of a West China lady's slipper is characterized in that being finished by following steps:

A, cultivation maternal plant: at the beginning of April, select 100 strain West China lady's slipper band soil muds to the accurate modern greenhouse of control, band soil one is many, can be with a small amount of accompanying plant; The rate of sheltering from heat or light reaches 50-60%, in time waters, and air humidity reaches 65-75%, and lady's slipper other trench digging in every strain West China is executed the acidic fertilizier diammonium phosphate of 1.5-2Kg, keeps P in soil H between 5.5-6.2, grows continuously 12-15 days, in time the removing of dead, pathology;

B, keep down the vines of a creeping plant: the West China lady's slipper of surviving in the above-mentioned steps is carried out plant division handle, with the other loosing soil of plant, execute the chicken manure 4-5Kg post-trenching that becomes thoroughly decomposed, disposable filling is permeable; Second day, side ridging 4-5cm in the ditch was thick to eugonic branch, notes being sure not to break branch, with 200-500 times of liquid pouring back normal management of 0.1-1.0% zeatin;

C, final singling in time: after the generation secondary root of keeping down the vines of a creeping plant, weak seedling is extractd in thinning in time, and excessive growth seedling and diseased plant stay robust growth, anosis plant, in the other ridging all around of strain, impels its upwards growth, waters;

D, greenhouse management: begin normal management after the final singling, the greenhouse rate of sheltering from heat or light remains on 50-55%, dark processing 6 hours, all the other full exposures; Temperature is 20-23 ℃ of daytime, remains on 16-18 ℃ evening; Keep down the vines of a creeping plant just to begin to reduce and water, air humidity is that 50-60% get final product, produces behind the new root suitably increase irrigation volume; Topdress in time, used 1000-1500 potassium dihydrogen phosphate aqueous solution doubly, and applied with water in every 7-10 days; The dressing time that staggers sprays how clever bacterium or the tpn of 10-20%, pre-preventing disease and pest;