CN112715310B - Transplanting method for ensuring current year breeding of cypripedium northeast - Google Patents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
- A01G22/63—Orchids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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Abstract
The invention relates to a transplanting method for ensuring the current year of the cypripedium northeast, which adopts the scheme that dark brown soil suitable for the introduction and cultivation of the cypripedium northeast is collected, symbiotic fungus source soil is collected from the field, humic soil, vermiculite, perlite and northeast peat soil which are rich in humus are mixed to form a regulating matrix, and finally the used transplanting soil is formed by fully mixing the fungus source soil containing the symbiotic fungus source, the dark brown soil and the regulating matrix according to the volume ratio of 1-2:6-8:1-2; during transplanting, selecting field clusters of cypripedium northeast with good growth, reserving more than 2 plants, transplanting the rest plants with soil and root systems, wherein the root systems contain endophytic fungus sources; transplanting is carried out in 5-8 months each year, shading and water management are carried out after transplanting, artificial pollination treatment is carried out on the cypripedium northeast at the flowering phase, the pollination success rate is improved, and after 2 months of pollination success, the next generation breeding can be carried out. The invention can improve the transplanting survival rate of field cypripedium faberi, and can ensure that the cypripedium faberi can be bred in the same year, thereby providing a wide application prospect for cypripedium faberi.
Description
Technical Field
The invention relates to the technical field of flower cultivation, in particular to a transplanting method for guaranteeing current year breeding of cypripedium arum.
Background
Cypripedium northeast (school name: cypripedium x ventricosum Sw.): plants up to 50 cm. The stem stands upright, usually with 3-5 leaves. Blade ovals to oval ovals. Inflorescences are topped, and 2 flowers are usually provided; flower red-purple, pink-white, and large size change; the petals are usually twisted somewhat; deep saccular, elliptic or inverted egg-shaped spherical, and usually a light ring is arranged around the saccule opening; the degenerated stamen can be up to 1 cm long. The flowering period is 5-6 months. The cypripedium northeast is a natural hybrid of cypripedium macranthus (C.macronts) and cypripedium macranthus (C.calceolus), the cypripedium macranthus group and cypripedium macranthus subgroup flowers meet in flowering phase, natural hybridization phenomenon exists in the same distribution area, and the color of the filial generation is changeable, so that the cypripedium northeast is a high-quality wild flower with extremely high potential and an important breeding resource. The cypripedium plant is mycorrhizal fungi symbiotic plant, is grown under the forest with the altitude of 200-2000 m in northeast area, has the color of deep purple, purple red, light powder to white and wide variation range, and is a high-quality wild flower with cultivation and development potential.
The wild living environment of the cypripedium northeast and the plant body have two main fungi, namely endophytic mycorrhizal fungi and symbiotic mycorrhizal fungi, so that the cypripedium northeast has difficulty in the propagation process and can be successfully introduced and cultivated. The cypripedium plant is the orchid with mycorrhizal fungi which provide necessary nutrition for the growth and development of the cypripedium plant, but the cypripedium plant is an autotrophic plant, the symbiotic condition of the cypripedium plant and the mycorrhizal fungi is not clear, and the open field cultivation technology is not mature. The published patent application CN11080583 discloses a preparation method of a cypripedium macranthum introduction culture medium, but the method has the advantages of longer time period for preparing the culture medium and high culture cost, and can not ensure that cypripedium macranthum can be set and bred in the current year after cypripedium macranthum is transplanted.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a transplanting method for ensuring that the cypripedium northeast can be bred in the same year, so that not only is the survival rate of cypripedium northeast increased, but also the cypripedium northeast transplanted in the same year can be matured and bred in the same year.
The invention comprises the following technical scheme:
a transplanting method for ensuring the current year of cypripedium northeast, comprising the following steps:
s1, collecting symbiotic fungus source soil
Collecting rhizosphere soil from the range of the root depth of a soil layer of 5-20cm and the deepest depth of the root system of the wild cypripedium northeast, wherein the rhizosphere soil contains symbiotic fungus sources;
s2, collecting dark brown soil
S3, collecting the leaf soil
Collecting natural leaf rot soil at a position close to the bottom of a valley in a forest, removing fallen leaves with non-rotten surface layers, and collecting a brown soft soil layer for later use;
s4, preparing a regulating matrix
The adjusting matrix comprises the leaf soil, vermiculite, perlite and northeast peat soil in the step S3, and the leaf soil, the vermiculite, the perlite and the northeast peat soil are uniformly mixed according to the volume ratio of 10-20:1-2:1-2:1-2;
the vermiculite is a mixture of vermiculite with the particle size of 2-4mm, 1-2mm and less than 1 mm;
the perlite is perlite with granularity of 60-80 meshes;
s5, introducing and cultivating
The transplanting soil is prepared by fully and uniformly mixing fungus source soil containing symbiotic fungus sources, dark brown soil and an adjusting matrix, wherein the fungus source soil is collected in the step S1, the dark brown soil is collected in the step S2, and the adjusting matrix is prepared in the step S4 according to the volume ratio of 1-2:6-8:1-2;
selecting a field population of cypripedium northeast with good growth, wherein the good growth judging method comprises the following steps: each cluster of plants is larger than 6 plants, and more than 2 plants are flowering and are considered to grow well;
during transplanting, more than 2 plants are reserved in the field, the rest plants are transplanted with soil and root systems, and the root systems contain endophytic fungus sources; transplantation is selected in the growing season of cypripedium, i.e. 5-8 months per year;
s6, post-transplanting management
Shading: carrying out shading treatment for 50% -75% of the cypripedium northeast plants transplanted from the field for one week, and removing the shading after one week for open-air cultivation;
and (3) watering: watering in time after transplanting to ensure that the moisture content of rhizosphere soil reaches more than 40%; watering once every other week, wherein each watering is used for wetting rhizosphere soil;
artificial pollination: artificial pollination is carried out on cypripedium northeast at the flowering stage, so that the current year is ensured to be firm;
s7, sowing or collecting seeds in situ after setting.
According to the preferred embodiment of the invention, in S1, the bacteria source in the soil 2-5cm below the ground in the rhizosphere range of wild cypripedium northeast is too little, and the humus is high, so that the bacteria source is removed; and the rhizosphere soil obtained in the range of 5-20cm soil layer in the rhizosphere range of wild cypripedium northeast and the cypripedium northeast colonitis contains more symbiotic fungi and can be collected as a bacterial source.
According to the preferred embodiment of the invention, in S2, the dark brown soil is taken from dark brown soil under the Mongolian oak secondary forest or under the Mongolian oak pine needle broad hybrid forest of the Minghan mountain, the Changbai mountain, the Wandashan mountain or the Dacheng mountain east slope and is used for the main soil composition of the cultivation soil.
According to the preferred embodiment of the present invention, in S3, the leaf-rot soil is leaf-rot soil with 2-5cm dead branches and fallen leaves under the Mongolian oak forest or the hazelnut forest of the east slope of the lesser Khingan, the Changbai mountain, the Wandashan or the great Khingan, and the dead branches and fallen leaves with the non-rotten surface layer are removed during collection, and the brown soft soil layer is obtained for use.
According to the preferred embodiment of the invention, in S4, vermiculite and perlite are used as soil conditioners to prevent soil hardening and increase air permeability. The peat soil produced in northeast China has good air permeability, high water holding capacity, light weight and balanced nutrition, and is used as a carbon source of a cultivation substrate to provide nutrition.
According to a preferred embodiment of the present invention, in S5, the transplanting of cypripedium northeast is performed at an ambient temperature of 18 ℃ or higher in the first two weeks of the last week of 5 months to 6 months of the year.
According to a preferred embodiment of the present invention, in S6, the method of artificial pollination is: and taking out the pollen grains of the arytenocypris northeast, and adhering the pollen grains to the back of the adhesive stamen column to complete artificial pollination.
Preferably, the pollen grains of the cypripedium northeast are removed as 15 # high precision curved mouth forceps. After pollination, the ovary of cypripedium northeast begins to expand within 15-20 days, namely the pollination is successful. The success rate of artificial pollination is over 90 percent, which is far greater than that of natural pollination (about 1-2 percent).
According to the preferred embodiment of the invention, in S7, after the seed maturation of the cypripedium northeast is successful for 2 months, the pericarp is cracked after the seed maturation, all seeds in the pericarp after the cracking are uniformly sown near the root of the cypripedium northeast, and symbiotic fungi capable of providing nutrition for the germination of the cypripedium northeast seeds are in the root soil, namely the current year sowing is completed; after vernalization and temperature change treatment, the cypripedium northeast seeds reaching the germination condition can germinate when the cypripedium northeast seeds have the germination condition in the second year or the third year, and the sowing and propagation of cypripedium arvensis seeds are completed; or collecting seeds, and culturing in a specific culture medium in a laboratory to assist germination.
The invention has the technical effects that:
the invention collects dark brown soil suitable for the introduction and cultivation of the cypripedium northeast, collects symbiotic fungus source soil from the field, mixes the soil with humic substances, vermiculite, perlite and the peat soil to form an adjusting matrix, and finally uses transplanting soil which is formed by fully mixing fungus source soil containing symbiotic fungus source, dark brown soil and adjusting matrix according to the volume ratio of 1-2:6-8:1-2; during transplanting, selecting field clusters of cypripedium northeast with good growth, reserving more than 2 plants, transplanting the rest plants with soil and root systems, wherein the root systems contain endophytic fungus sources; transplanting is carried out in 5-8 months each year, shading and water management are carried out after transplanting, artificial pollination treatment is carried out on the cypripedium northeast at the flowering phase, the pollination success rate is improved, and after 2 months of pollination success, the next generation breeding can be carried out.
The invention can improve the transplanting survival rate of field cypripedium faberi, and can ensure that the cypripedium faberi can be bred in the same year, thereby providing a wide application prospect for cypripedium faberi.
Detailed Description
In order to further explain the technical effects of the present invention, specific examples are described below.
Example 1
The invention provides a transplanting method for ensuring that cypripedium northeast can be bred in the current year, which comprises the following steps:
(1) Collecting symbiotic fungus source soil
Collecting rhizosphere soil from the range of the root depth of a soil layer of 5-20cm and the deepest depth of the root system of the wild cypripedium northeast, wherein the rhizosphere soil contains symbiotic fungus sources;
the fungus sources in the soil 2-5cm below the ground in the rhizosphere range of the wild cypripedium northeast, and the humus is high, so that the fungus sources are removed; and the rhizosphere soil obtained in the range of 5-20cm soil layer in the rhizosphere range of wild cypripedium northeast and the cypripedium northeast colonitis contains more symbiotic fungi and can be collected as a bacterial source.
(2) Collecting dark brown soil
The dark brown soil is taken from the dark brown soil under the Mongolian oak secondary forest or the Mongolian oak red pine needle broad hybrid forest of the little Xingan, and is used for the main soil composition of the cultivation soil.
(3) Collecting leaf soil
The leaf rot soil is leaf rot soil with 2-5cm dead branches and fallen leaves under the hazelnut forest from the little Khingan, natural leaf rot soil is collected at a position close to the bottom of the gullies in the forest, fallen leaves with non-rotten surface layers are removed, and a brown soft soil layer is collected for standby.
(4) Preparation of a conditioning matrix
The adjusting matrix comprises the leaf soil, vermiculite, perlite and northeast peat soil in the step (3), and the leaf soil, the vermiculite, the perlite and the northeast peat soil are uniformly mixed according to the volume ratio of 15:1:1:1. Vermiculite is a mixture of vermiculite with the grain diameter of 2-4mm, 1-2mm and less than 1 mm. The perlite is perlite with the granularity of 80 meshes.
Vermiculite and perlite are used as soil conditioners to prevent soil hardening and increase air permeability and water permeability. The peat soil produced in northeast China has good air permeability, high water holding capacity, light weight and balanced nutrition, and is used as a carbon source of a cultivation substrate to provide nutrition.
(5) Introduction and cultivation
The transplanting soil is prepared by fully and uniformly mixing fungus source soil containing symbiotic fungus sources, which is collected in the step (1), dark brown soil and an adjusting matrix, which are prepared in the step (4), according to the volume ratio of 2:6:2. Selecting a field population of cypripedium northeast with good growth, wherein the good growth judging method comprises the following steps: more than 6 plants per cluster and more than 2 flowers are considered to grow well.
During transplanting, more than 2 plants are reserved in the field, the rest plants are transplanted with soil and root systems, and the root systems contain endophytic fungus sources; the transplantation selection is performed in the growing season of cypripedium, i.e. the last week of 5 months of the year.
S6, post-transplanting management
Shading: 75% shading treatment is carried out on the cypripedium northeast plant transplanted from the field for one week, and the shading is removed after one week for open-air cultivation;
and (3) watering: watering in time after transplanting to ensure that the moisture content of rhizosphere soil reaches more than 40%; watering once every other week, wherein each watering is used for wetting rhizosphere soil;
artificial pollination: artificial pollination is carried out on cypripedium northeast at the flowering stage, so that the current year is ensured to be firm;
during pollination, the pollen grains of the North-east arytenocypris cypress are manually removed by using 15-number high-precision bent-mouth tweezers, and the pollen grains are stuck to the back of the sticky stamen column, so that the artificial pollination is completed. After pollination, the ovary of cypripedium northeast begins to expand within 15-20 days, namely the pollination is successful. The success rate of artificial pollination is over 90 percent, which is far greater than that of natural pollination (about 1-2 percent).
S7, sowing or collecting seeds in situ after setting
After the seed maturation of the cypripedium northeast is successful for 2 months, the pericarp can crack after the seed maturation, all seeds in the pericarp after the cracking are uniformly sown near the root of the cypripedium northeast, and symbiotic fungi capable of providing nutrition for the germination of the cypripedium northeast seeds are in the root soil, so that the sowing in the current year is completed; after vernalization and temperature change treatment, the cypripedium northeast seeds reaching the germination condition can germinate when the cypripedium northeast seeds have the germination condition in the second year or the third year, and the sowing and propagation of cypripedium arvensis seeds are completed; or collecting seeds, and culturing in a specific culture medium in a laboratory to assist germination.
Example 2
The invention provides a transplanting method for ensuring that cypripedium northeast can be bred in the current year, which comprises the following steps:
(1) Collecting symbiotic fungus source soil
Collecting rhizosphere soil from the range of the root depth of a soil layer of 5-20cm and the deepest depth of the root system of the wild cypripedium northeast, wherein the rhizosphere soil contains symbiotic fungus sources;
the fungus sources in the soil 2-5cm below the ground in the rhizosphere range of the wild cypripedium northeast, and the humus is high, so that the fungus sources are removed; and the rhizosphere soil obtained in the range of 5-20cm soil layer in the rhizosphere range of wild cypripedium northeast and the cypripedium northeast colonitis contains more symbiotic fungi and can be collected as a bacterial source.
(2) Collecting dark brown soil
The dark brown soil is taken from the dark brown soil under the Mongolian oak secondary forest or the Mongolian oak red pine needle broad hybrid forest of the little Xingan, and is used for the main soil composition of the cultivation soil.
(3) Collecting leaf soil
The leaf rot soil is leaf rot soil with 2-5cm dead branches and fallen leaves under a Mongolian oak forest taken from the lesser Khingan, natural leaf rot soil is collected at a position close to the bottom of a valley in the forest, fallen leaves with non-rotten surface layers are removed, and a brown soft soil layer is collected for standby.
(4) Preparation of a conditioning matrix
The adjusting matrix comprises the leaf soil, vermiculite, perlite and northeast peat soil in the step (3), and the leaf soil, the vermiculite, the perlite and the northeast peat soil are uniformly mixed according to the volume ratio of 20:2:2:2. The vermiculite is the mixture of vermiculite with the grain diameter of 2-4mm, 1-2mm and less than 1mm, and the perlite is the perlite with the grain diameter of 70 meshes.
Vermiculite and perlite are used as soil conditioners to prevent soil hardening and increase air permeability and water permeability. The peat soil produced in northeast China has good air permeability, high water holding capacity, light weight and balanced nutrition, and is used as a carbon source of a cultivation substrate to provide nutrition.
(5) Introduction and cultivation
The transplanting soil is prepared by fully and uniformly mixing fungus source soil containing symbiotic fungus sources, which is collected in the step (1), dark brown soil and an adjusting matrix, which are prepared in the step (4), according to the volume ratio of 1:8:1. Selecting a field population of cypripedium northeast with good growth, wherein the good growth judging method comprises the following steps: more than 6 plants per cluster and more than 2 flowers are considered to grow well.
During transplanting, more than 2 plants are reserved in the field, the rest plants are transplanted with soil and root systems, and the root systems contain endophytic fungus sources; transplantation selection is performed in the growing season of cypripedium, i.e. before the middle 6 months of the year.
S6, post-transplanting management
Shading: carrying out a shading treatment of 65% of the cypripedium northeast plant transplanted from the field for one week, and removing the shading after one week for open-air cultivation;
and (3) watering: watering in time after transplanting to ensure that the moisture content of rhizosphere soil reaches more than 40%; watering once every other week, wherein each watering is used for wetting rhizosphere soil;
artificial pollination: artificial pollination is carried out on cypripedium northeast at the flowering stage, so that the current year is ensured to be firm;
during pollination, the pollen grains of the North-east arytenocypris cypress are manually removed by using 15-number high-precision bent-mouth tweezers, and the pollen grains are stuck to the back of the sticky stamen column, so that the artificial pollination is completed. After pollination, the ovary of cypripedium northeast begins to expand within 15-20 days, namely the pollination is successful.
S7, sowing or collecting seeds in situ after setting
After the seed maturation of the cypripedium northeast is successful for 2 months, the pericarp can crack after the seed maturation, all seeds in the pericarp after the cracking are uniformly sown near the root of the cypripedium northeast, and symbiotic fungi capable of providing nutrition for the germination of the cypripedium northeast seeds are in the root soil, so that the sowing in the current year is completed; after vernalization and temperature change treatment, the cypripedium northeast seeds reaching the germination condition can germinate when the cypripedium northeast seeds have the germination condition in the second year or the third year, and the sowing and propagation of cypripedium arvensis seeds are completed; or collecting seeds, and culturing in a specific culture medium in a laboratory to assist germination.
Comparative example 1
Based on the example 1, the comparative example did not collect the symbiotic fungus source soil, and the dark brown soil was used to replace the symbiotic fungus source soil when preparing the transplanting soil.
Comparative example 2
On the basis of example 1, in the preparation of the conditioning substrate, the leaf mold was not used, but instead vermiculite and perlite were used in place of the leaf mold.
Comparative example 3
On the basis of example 1, dark brown soil is not collected, and when transplanting soil is prepared, the dark brown soil is replaced by leaf rot soil.
Comparative example 4
On the basis of example 1, northeast peat soil was not used in the preparation of the conditioned medium, but instead the northeast peat soil was replaced with vermiculite and perlite.
Comparative example 5
On the basis of example 1, the plants were cultivated in the open air without shading after transplanting.
300 plants of cypripedium northeast were transplanted respectively according to the above method, and the survival rate and the fruiting rate of cypripedium northeast cultivated in the above examples and comparative examples in the current year were counted as shown in the following table.
| Group of | Survival rate of | Results rate |
| Example 1 | 98％ | 96％ |
| Example 2 | 95％ | 94％ |
| Comparative example 1 | 44％ | 17％ |
| Comparative example 2 | 72％ | 62％ |
| Comparative example 3 | 60％ | 48％ |
| Comparative example 4 | 76％ | 65％ |
| Comparative example 5 | 33％ | 14％ |
The above examples are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solution of the present invention should fall within the scope of protection defined by the claims of the present invention without departing from the spirit of the present invention.
Claims (6)
1. A transplanting method for ensuring the current year of cypripedium northeast, which is characterized by comprising the following steps:
s1, collecting symbiotic fungus source soil
Collecting rhizosphere soil from the range of the root depth of a soil layer of 5-20cm and the deepest depth of the root system of the wild cypripedium northeast, wherein the rhizosphere soil contains symbiotic fungus sources;
s2, collecting dark brown soil
S3, collecting the leaf soil
Collecting natural leaf rot soil at a position close to the bottom of a valley in a forest, removing fallen leaves with non-rotten surface layers, and collecting a brown soft soil layer for later use;
s4, preparing a regulating matrix
The adjusting matrix comprises the leaf soil, vermiculite, perlite and northeast peat soil in the step S3, and the leaf soil, the vermiculite, the perlite and the northeast peat soil are uniformly mixed according to the volume ratio of 10-20:1-2:1-2:1-2;
the vermiculite is a mixture of vermiculite with the particle size of 2-4mm, 1-2mm and less than 1 mm;
the perlite is perlite with granularity of 60-80 meshes;
s5, introducing and cultivating
The transplanting soil is prepared by fully and uniformly mixing fungus source soil containing symbiotic fungus sources, dark brown soil and an adjusting matrix, wherein the fungus source soil is collected in the step S1, the dark brown soil is collected in the step S2, and the adjusting matrix is prepared in the step S4 according to the volume ratio of 1-2:6-8:1-2;
selecting a field population of cypripedium northeast with good growth, wherein the good growth judging method comprises the following steps: each cluster of plants is larger than 6 plants, and more than 2 plants are flowering and are considered to grow well;
during transplanting, more than 2 plants are reserved in the field, the rest plants are transplanted with soil and root systems, and the root systems contain endophytic fungus sources; transplantation is selected in the growing season of cypripedium, i.e. 5-8 months per year;
s6, post-transplanting management
Shading: carrying out shading treatment for 50% -75% of the cypripedium northeast plants transplanted from the field for one week, and removing the shading after one week for open-air cultivation;
and (3) watering: watering in time after transplanting to ensure that the moisture content of rhizosphere soil reaches more than 40%; watering once every other week, wherein each watering is used for wetting rhizosphere soil;
artificial pollination: artificial pollination is carried out on cypripedium northeast at the flowering stage, so that the current year is ensured to be firm;
s7: sowing or collecting seeds in situ after setting.
2. The method according to claim 1, wherein in S2, the dark brown soil is taken from dark brown soil under the secondary forest of mongolian oak or under the broad hybrid forest of mongolian oak red pine needles in the downhill of the lesser Khingan, the Changbai mountain, the Wandashan or the great Khingan.
3. The method according to claim 1, wherein in S3, the leaf soil is leaf soil with 2-5cm dead branches and fallen leaves under the Mongolian oak forest or hazelnut forest taken from the east slope of the lesser Khingan mountain, the Changbai mountain, the Wandashan mountain or the great Khingan mountain, and when collecting, the dead branches and fallen leaves with the non-rotten surface layer are removed, and the brown soft soil layer is taken for use.
4. The method according to claim 1, wherein in S5, the transplanting of cypripedium northeast is performed at 18 ℃ or higher in the last week of 5 months to the first two weeks of 6 months each year.
5. The method according to claim 1, wherein in S6, the artificial pollination is performed by: and taking out the pollen grains of the arytenocypris northeast, and adhering the pollen grains to the back of the adhesive stamen column to complete artificial pollination.
6. The method according to claim 1, wherein in S7, after the seed ripening of the cypripedium northeast is successful for 2 months, the pericarp will crack after the seed ripening, and all the seeds in the pericarp after the crack are uniformly spread near the root of cypripedium arvensis, and the root soil contains symbiotic fungi which can provide nutrition for the germination of cypripedium northeast seeds, thus completing the current year of sowing; after vernalization and temperature change treatment, the cypripedium northeast seeds reaching the germination condition can germinate when the cypripedium northeast seeds have the germination condition in the second year or the third year, and the sowing and propagation of cypripedium arvensis seeds are completed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110001812.8A CN112715310B (en) | 2021-01-04 | 2021-01-04 | Transplanting method for ensuring current year breeding of cypripedium northeast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110001812.8A CN112715310B (en) | 2021-01-04 | 2021-01-04 | Transplanting method for ensuring current year breeding of cypripedium northeast |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103181283A (en) * | 2011-12-31 | 2013-07-03 | 何聪 | Method for acclimatizing cypripedium farreri |
| CN106577284A (en) * | 2016-12-15 | 2017-04-26 | 四川省自然资源科学研究院 | Method for tissue culture, rapid propagation and flowering time regulation of cypripedium flavum |
| CN110800583A (en) * | 2019-12-02 | 2020-02-18 | 吉林农业大学 | Preparation method of cypripedium macranthum introduction culture medium |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103181283A (en) * | 2011-12-31 | 2013-07-03 | 何聪 | Method for acclimatizing cypripedium farreri |
| CN106577284A (en) * | 2016-12-15 | 2017-04-26 | 四川省自然资源科学研究院 | Method for tissue culture, rapid propagation and flowering time regulation of cypripedium flavum |
| CN110800583A (en) * | 2019-12-02 | 2020-02-18 | 吉林农业大学 | Preparation method of cypripedium macranthum introduction culture medium |
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