CN1031722C - 用于测定α-淀粉酶活性的试剂和方法 - Google Patents
用于测定α-淀粉酶活性的试剂和方法 Download PDFInfo
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- CN1031722C CN1031722C CN 92105096 CN92105096A CN1031722C CN 1031722 C CN1031722 C CN 1031722C CN 92105096 CN92105096 CN 92105096 CN 92105096 A CN92105096 A CN 92105096A CN 1031722 C CN1031722 C CN 1031722C
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- Prior art keywords
- acetyl
- nitrophenyl
- chlorine
- amylase activity
- glucopyranosyl
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Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
Description
α—淀粉酶活性(IU/l) | 在2分钟内吸光度的增加(通过0IU/l的α—淀粉酶活性加以校正) | |
底物溶液(a)(本发明的底物溶液) | 底物溶液(b)(参照底物溶液) | |
250 | 0.058 | 0.002 |
500 | 0.118 | 0.005 |
α—淀粉酶活性(IU/l) | 在2分钟内吸光度的增加(通过OIU/l的α—淀粉酶活性加以校正) | |
底物溶液(a)(本发明的底物溶液) | 底物溶液(b)(参照底物溶液) | |
250 | 0.057 | 0.001 |
500 | 0.116 | 0.002 |
底 物 | Am | Km值(mM) | 水解速度 | 水溶性 |
EMG5CNP | P | 0.13 | 10 | 好 |
S | 0.14 | 10 | ||
ADG5CNP | P | 0.17 | 10 | 好 |
S | 0.26 | 10 | ||
G5CNP | P | 0.29 | 10 | 好 |
S | 0.37 | 10 |
底 物 | Am | 作用方式〔水解产物(mol%)〕 | |||
G4CNP | G3GNP | G2CNP | GCNP | ||
EMG5CNP | P | 0 | 0 | 97 | 3 |
S | 0 | 0 | 97 | 3 | |
ADG5CNP | P | 0 | 3 | 97 | 0 |
S | 0 | 0 | 100 | 0 | |
G5CNP | P | 8 | 8 | 81 | 3 |
S | 4 | 11 | 83 | 2 |
Claims (4)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP18043791A JPH051234A (ja) | 1991-06-26 | 1991-06-26 | ジオスコレア・アラ−タからの抽出色素 |
JP180437/91 | 1991-06-26 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1067896A CN1067896A (zh) | 1993-01-13 |
CN1031722C true CN1031722C (zh) | 1996-05-01 |
Family
ID=16083227
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 92105096 Expired - Fee Related CN1031722C (zh) | 1991-06-26 | 1992-06-25 | 用于测定α-淀粉酶活性的试剂和方法 |
Country Status (2)
Country | Link |
---|---|
JP (1) | JPH051234A (zh) |
CN (1) | CN1031722C (zh) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060068036A1 (en) * | 2002-12-31 | 2006-03-30 | National Yang-Ming University | Extract of Dioscorea sp. and the medical uses thereof |
CN103898193B (zh) * | 2014-03-24 | 2017-03-29 | 湖南大学 | α‑淀粉酶的检测试剂和检测方法 |
CN104132939A (zh) * | 2014-07-12 | 2014-11-05 | 福建农林大学 | 一种蜂蜜中淀粉酶值的快速检测方法 |
CN110187106A (zh) * | 2019-05-31 | 2019-08-30 | 北京石油化工学院 | 一种定量测定α-淀粉酶活性的多层膜干片及其测定方法 |
-
1991
- 1991-06-26 JP JP18043791A patent/JPH051234A/ja active Pending
-
1992
- 1992-06-25 CN CN 92105096 patent/CN1031722C/zh not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CN1067896A (zh) | 1993-01-13 |
JPH051234A (ja) | 1993-01-08 |
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