CN103169773A - Traditional Chinese medicine compound preparation for enhancing livestock immunity and preparation method - Google Patents
Traditional Chinese medicine compound preparation for enhancing livestock immunity and preparation method Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine compound preparation for enhancing livestock immunity, which comprises 1-5 parts of Radix Astragali, 1-5 parts of Radix Codonopsis and 1-5 parts of ligustrum lucidum, and the application forms can be a traditional Chinese medicine powder, an oral liquid, a particulate agent and an injection. The traditional Chinese medicine compound preparation for enhancing livestock immunity can be used for enhancing the livestock immunity functions of chicken, ducks, gooses, pigs and sheep; and especially, can be used for enhancing the immunization effect of vaccines such as newcastle disease vaccine or hog cholera vaccine. The traditional Chinese medicine compound preparation before and after the animal immunization and the vaccine are simultaneously used, the immunization function of animal body can be enhanced, and the immunization effect of the vaccine can be enhanced.
Description
Technical field
The present invention relates to a kind of compound Chinese medicinal preparation that strengthens livestock immunity and preparation method thereof, belong to the field of Chinese medicines.
Background technology
Intensification along with development and the intensive degree of livestock and poultry breeding industry, the growing environment of poultry is constantly improved, fertility performance is farthest brought into play, simultaneously, activity is restricted, and adds high-caliber fertility performance, and poultry self-regulation function is lower, resistance is poor, the several factors in feeding and management such as Changes in weather, cuts off the water and electricity supply, changes keeper, vaccine immunity etc. and all may become the inducement of its morbidity.
Present stage, traditional cultivation idea has not existed, and the culturist generally adheres to the policy of " put prevention first, anti-overweighting controlled ", and for the prevention and control of disease, people mainly rely on the immunity inoculation of vaccine, the key point of vaccine immunity for deciding the livestock and poultry cultivation success or failure.But in recent years, development along with aquaculture, the immunosuppressive disease sickness rate increases year by year, when poultry have infected these diseases such as infectious bursal disease, avian leukosis, Porcine circovirus desease, sow reproductive respiratory syndrome, the fowl poultry immune systemic-function is impaired, the antigen responsibility is descended, affect the immune effect of vaccine of other diseases, cause immuning failure.
Vaccine immunity unsuccessfully causes the vaccine consumption to increase, drug dose increases, the animal products that this mode is produced threatens to human food's health, more make the low immunologic function of poultry script constantly descend, the function of immune system of poultry is in inhibitory state, the normal immunologic barrier effect of poultry body dies down, and is difficult to resist the invasion and attack of disease.
Summary of the invention
In view of this, main purpose of the present invention is to provide a kind of compound Chinese medicinal preparation that strengthens livestock immunity and preparation method thereof, to realize improving livestock immunity and to improve the effect of vaccine in the immunity of fowl poultry.
Technical scheme
A kind of compound Chinese medicinal preparation that strengthens livestock immunity consists of the following composition: Radix Astragali 1-5 part, Radix Codonopsis 1-5 part, Fructus Ligustri Lucidi 1-5 part.
The present invention strengthens the compound Chinese medicinal preparation of poultry immunity, and preferred version is:
Radix Astragali 2-3 part, Radix Codonopsis 2-3 part, Fructus Ligustri Lucidi 2-3 part.
The present invention strengthens the Chinese prescription of animal immunizing power, and its application form is: Chinese powder medicine, oral liquid, granule, injection.Its preparation method is as follows:
Chinese powder medicine: get Radix Astragali 2-5 part, Radix Codonopsis 2-5 part, Fructus Ligustri Lucidi 1-5 part, pulverize, cross 100 mesh sieves, mix homogeneously, and get final product.
The preparation method of other dosage forms is as follows:
(1) get the Radix Astragali, Fructus Ligustri Lucidi, add the ethanol 6-10 of 65-80% doubly to measure by medical material weight, dynamically reflux, extract, 4h, extract and finish, extracting solution is substantially colourless, merge extractive liquid,, the 10000-20000r/min high speed centrifugation is removed impurity, is evaporated to without the alcohol flavor, gets the Radix Astragali, Fructus Ligustri Lucidi extract.
(2) get Radix Codonopsis, add 8-12 times of water gaging by medical material weight, vacuum back-flow extracts 4h, extraction concentrates simultaneously, extracts and finishes, merge extractive liquid,, the 10000-20000r/min high speed centrifugation is removed impurity, continues to be evaporated to relative density 1.10-1.25, gets Radix Codonopsis extract.
(3) merge above extracting solution, extracting solution is concentrated into the clear paste that every ml contains primary crude drug 1-1.5g, adding 1mol/LNaOH to regulate pH value is 5.0-7.0, namely gets compound Chinese medicine extract.
(4) get (1)-Chinese medicine extract that (3) make, preparation oral liquid, granule, injection, the preparation method of each drug form is as follows:
1. oral liquid is got the Chinese medicine extract that above-mentioned (3) make, and is diluted with water to every milliliter and contains primary crude drug 1.2-1.5g, filter, and fill, 115 ℃ of high pressure 30min sterilize, and namely get the herbal mixture oral liquid.
2. granule is got the Chinese medicine extract that above-mentioned (3) make, cryoconcentration (40-60 ℃) is concentrated into the thick paste that relative density is 1.30-1.35, adds sucrose and dextrin, and cream is heavy, the ratio of sucrose, dextrin is 1: 2: 1, granulation carries out namely getting the herbal mixture granule after drying.
3. injection is got the Chinese medicine extract that above-mentioned (3) make, and is diluted with water to every milliliter and contains primary crude drug 1-1.2g, adds the injection charcoal in the 0.5-1% ratio, stirs 30-60min, filters, and removes impurity.Filtrate injects water to scale with aperture 0.45 μ m filter membrane fine straining, regulates pH value, fill, and sterilization namely gets the herbal mixture injection.
The present invention strengthens the common formulations of the Chinese prescription of animal immunizing power, and oral liquid, granule, injection calculate the content of the Radix Astragali with astragaloside, and wherein oral liquid, the every ml of injection contain astragaloside and must not be less than 0.2mg.
Chinese herbal compound preparation of the present invention, usage and dosage is as follows:
Powder is 0.5-1kg spice 100kg, and oral liquid 1-2ml is watered 1kg, and granule is that 1-2g is watered 1kg, tablet 1-2 sheet/plumage, injection pig, sheep 0.1ml/kg, chicken, duck, goose 0.2ml/kg.
As seen from the above, the present invention's compound Chinese medicinal preparation of strengthening livestock immunity can strengthen the application of immunologic function of livestock and birds aspect; More preferably, strengthen the application of the domestic animal immunologic function such as the poultry such as chicken, duck, goose and pig, sheep aspect; Most preferably, be in the application that strengthens aspect chicken, pig immunologic function; Particularly, for strengthening the immune effect of the vaccines such as ND Vaccine or swine Fever Vaccine.Be the compound Chinese medicinal preparation of the enhancing livestock immunity of the application of the invention, before and after the animal immune or use simultaneously with vaccine, can strengthen the immunologic function of animal body, strengthen the immune effect of vaccine.
The specific embodiment
Embodiment 1 strengthens the preparation of the Chinese traditional compound medicine of livestock immunity
Be below the preparation example of Chinese traditional compound medicine of the present invention:
The Chinese powder medicine of preparation example 1, Chinese traditional compound medicine
The Radix Astragali 2 parts of (2kg), Radix Codonopsis 2 parts of (2kg), 5 parts of Fructus Ligustri Lucidi (5kg), mix homogeneously adds crusher for crushing, stirs, and crosses 100 mesh sieves, and packing namely gets Chinese prescription powder 9kg of the present invention.
The Chinese powder medicine of preparation example 2, Chinese traditional compound medicine
The Radix Astragali 5 parts of (2kg), Radix Codonopsis 5 parts of (5kg), 5 parts of Fructus Ligustri Lucidi (5kg), mix homogeneously adds crusher for crushing, stirs, and crosses 100 mesh sieves, and packing namely gets Chinese prescription powder 15kg of the present invention.
The oral liquid formulation of preparation example 3, Chinese traditional compound medicine
Get the Radix Astragali 3 parts of (3kg), 4 parts of Fructus Ligustri Lucidi (4kg), add 10 times of amounts of ethanol of 70% by medical material weight, dynamic reflux, extract, 4h, extract and finish, extracting solution is substantially colourless, merge extractive liquid,, and the 16000r/min high speed centrifugation is removed impurity, be evaporated to without the alcohol flavor, get the Radix Astragali, Fructus Ligustri Lucidi extracting solution.
Get 3 parts of Radix Codonopsis (3kg), add 12 times of water gagings by medical material weight, vacuum back-flow extracts 4h, extraction concentrates simultaneously, extracts and finishes, merge extractive liquid,, the 15000r/min high speed centrifugation is removed impurity, continues to be evaporated to relative density 1.10-1.25, gets the Radix Codonopsis extracting solution.
Merge above extracting solution, extracting solution is concentrated into the clear paste that every ml contains primary crude drug 1-1.5g, adding 1mol/L NaOH to regulate pH value is 5.0-7.0, adds water to 10L, stir, cold preservation 48h proofreaies and correct pH, filters, bottling, sterilization namely gets Chinese prescription oral liquid of the present invention.
Get above-mentioned traditional Chinese medicine compound oral liquid, measure the content of astragaloside according to the detection method under two Radix Astragali items of People's Republic of China's veterinary drug allusion quotation, after measured, the every ml of this product contains astragaloside 0.39mg.
The oral liquid formulation of preparation example 4, Chinese traditional compound medicine
Get the Radix Astragali 2 parts of (2kg), 1 part of Fructus Ligustri Lucidi (1kg), add 10 times of amounts of ethanol of 70% by medical material weight, dynamic reflux, extract, 4h, extract and finish, extracting solution is substantially colourless, merge extractive liquid,, and the 16000r/min high speed centrifugation is removed impurity, be evaporated to without the alcohol flavor, get the Radix Astragali, Fructus Ligustri Lucidi extracting solution.
Get 2 parts of Radix Codonopsis (2kg), add 12 times of water gagings by medical material weight, vacuum back-flow extracts 4h, extraction concentrates simultaneously, extracts and finishes, merge extractive liquid,, the 17000r/min high speed centrifugation is removed impurity, continues to be evaporated to relative density 1.10-1.25, gets the Radix Codonopsis extracting solution.
Merge above extract, extracting solution is diluted to the extracting solution that every ml contains primary crude drug 1-1.5g, adding 1mol/L NaOH to regulate pH value is 5.0-7.0, adds water to 5L, stir, cold preservation 48h proofreaies and correct pH, filters, bottling, sterilization namely gets Chinese prescription oral liquid of the present invention.
Get above-mentioned traditional Chinese medicine compound oral liquid, measure the content of astragaloside according to the detection method under two Radix Astragali items of People's Republic of China's veterinary drug allusion quotation, after measured, the every ml of this product contains astragaloside 0.52mg.
The granule dosage form of preparation example 5, Chinese traditional compound medicine
Get the Radix Astragali 5 parts of (5kg), 2 parts of Fructus Ligustri Lucidi (2kg), add 8 times of amounts of ethanol of 75% by medical material weight, dynamic reflux, extract, 4h, extract and finish, extracting solution is substantially colourless, merge extractive liquid,, and the 18000r/min high speed centrifugation is removed impurity, be evaporated to without the alcohol flavor, get the Radix Astragali, Fructus Ligustri Lucidi extracting solution.
Get 2 parts of Radix Codonopsis (2kg), add 10 times of water gagings by medical material weight, vacuum back-flow extracts 4h, extraction concentrates simultaneously, extracts and finishes, merge extractive liquid,, the 16000r/min high speed centrifugation is removed impurity, continues to be evaporated to relative density 1.10-1.25, gets the Radix Codonopsis extracting solution.
Merge above extracting solution, filter, extracting solution is concentrated into the thick paste that relative density is 1.32-1.35, add sucrose and dextrin, the ratio that sucrose and dextrin and cream are heavy is 2: 1: 1, granulation, and drying namely gets Chinese prescription granule 9kg of the present invention.
Get above-mentioned Chinese prescription granule, dissolve, measure the content of astragaloside according to the detection method under two Radix Astragali items of People's Republic of China's veterinary drug allusion quotation, after measured, the every ml of this product contains astragaloside 0.68mg.
The granule dosage form of preparation example 6, Chinese traditional compound medicine
Get the Radix Astragali 2 parts of (2kg), 2 parts of Fructus Ligustri Lucidi (2kg), add 8 times of amounts of ethanol of 75% by medical material weight, dynamic reflux, extract, 4h, extract and finish, extracting solution is substantially colourless, merge extractive liquid,, and the 18000r/min high speed centrifugation is removed impurity, be evaporated to without the alcohol flavor, get the Radix Astragali, Fructus Ligustri Lucidi extracting solution.
Get 5 parts of Radix Codonopsis (5kg), add 10 times of water gagings by medical material weight, vacuum back-flow extracts 4h, extraction concentrates simultaneously, extracts and finishes, merge extractive liquid,, the 13000r/min high speed centrifugation is removed impurity, continues to be evaporated to relative density 1.10-1.25, gets the Radix Codonopsis extracting solution.
Merge above extracting solution, filter, extracting solution is concentrated into the thick paste that relative density is 1.32-1.35, add sucrose and dextrin, the ratio that sucrose and dextrin and cream are heavy is 2: 1: 1, granulation, and drying namely gets Chinese prescription granule 9kg of the present invention.
Get above-mentioned Chinese prescription granule, dissolve, measure the content of astragaloside according to the detection method under two Radix Astragali items of People's Republic of China's veterinary drug allusion quotation, after measured, the every ml of this product contains astragaloside 0.31mg.
The injection dosage form of preparation example 7, Chinese traditional compound medicine
Get the Radix Astragali 3 parts of (3kg), 3 parts of Fructus Ligustri Lucidi (3kg), add 8 times of amounts of ethanol of 70% by medical material weight, dynamic reflux, extract, 4h, extract and finish, extracting solution is substantially colourless, merge extractive liquid,, and the 18000r/min high speed centrifugation is removed impurity, be evaporated to without the alcohol flavor, get the Radix Astragali, Fructus Ligustri Lucidi extracting solution.
Get 3 parts of Radix Codonopsis (3kg), add 10 times of water gagings by medical material weight, vacuum back-flow extracts 4h, extraction concentrates simultaneously, extracts and finishes, merge extractive liquid,, the 16000r/min high speed centrifugation is removed impurity, continues to be evaporated to relative density 1.10-1.25, gets the Radix Codonopsis extracting solution.
Merge above extracting solution, filter, extracting solution is concentrated into the clear paste that relative density is 1.1-1.2, add 1% active carbon, stir 30-60min, filter, remove impurity.Filtrate injects water to 9L with aperture 0.45 μ m filter membrane fine straining, regulates pH value to 6.5 with the 1mol/L sodium hydroxide, fill, and sterilization namely gets Chinese prescription injection of the present invention.
Get above-mentioned Chinese prescription injection, measure the content of astragaloside according to the detection method under two Radix Astragali items of People's Republic of China's veterinary drug allusion quotation, after measured, the every ml of this product contains astragaloside 0.41mg.
The injection dosage form of preparation example 8, Chinese traditional compound medicine
Get the Radix Astragali 4 parts of (4kg), 3 parts of Fructus Ligustri Lucidi (3kg), add 8 times of amounts of ethanol of 70% by medical material weight, dynamic reflux, extract, 4h, extract and finish, extracting solution is substantially colourless, merge extractive liquid,, and the 18000r/min high speed centrifugation is removed impurity, be evaporated to without the alcohol flavor, get the Radix Astragali, Fructus Ligustri Lucidi extracting solution.
Get 4 parts of Radix Codonopsis (4kg), add 10 times of water gagings by medical material weight, vacuum back-flow extracts 4h, extraction concentrates simultaneously, extracts and finishes, merge extractive liquid,, the 18000r/min high speed centrifugation is removed impurity, continues to be evaporated to relative density 1.10-1.25, gets the Radix Codonopsis extracting solution.
Merge above extract, filter, extracting solution is concentrated into the clear paste that relative density is 1.1-1.2, add 1% active carbon, stir 30-60min, filter, remove impurity.Filtrate injects water to 11L with aperture 0.45 μ m filter membrane fine straining, regulates pH value to 6.8 with the 1mol/L sodium hydroxide, fill, and sterilization namely gets Chinese prescription injection of the present invention.
Get above-mentioned Chinese prescription injection, measure the content of astragaloside according to the detection method under two Radix Astragali items of People's Republic of China's veterinary drug allusion quotation, after measured, the every ml of this product contains astragaloside 0.47mg.
The impact of embodiment 2 compound Chinese medicinal preparation of the present invention on chicken immuological function
Adopt the compound Chinese medicinal preparation oral liquid formulation of preparation example 3 gained of the present invention, contrast with the Radix Astragali, dangshen oral liquid simultaneously, measure herbal mixture oral liquid of the present invention to the impact of chicken immuological function.The patent working example 2 that the Radix codonopsis and Radix Astragali oral liquid is CN 101940613A according to publication number is prepared, and the Radix Astragali, the dangshen oral liquid that will prepare gained concentrate, and makes every milliliters of liquid contain primary crude drug 1g.
1. the impact of prescription of the present invention on chicken immuological function
1 120 of age in days Avain chickens are divided into 3 groups, 40 every group.Normally raise to 7 ages in days, carry out administration by table 1 scheme, in 7d, 14d, 21d, the 28d blood sampling of first administration, mtt assay is measured the propagation situation of periphery blood T lymphocyte, randomly draw 5 for every group simultaneously, weigh, the rear blood-letting of numbering is lethal, gets its fabricius bursa, spleen, thymus, take weight, calculate each Immune Organs Index, and prepare the tissue slice of each immune organ, examine under a microscope its variation.
The concrete grouping of table 1 test and processing
(1) impact of the oral liquid of method preparation of the present invention on the Immune Organs of Chicken growth promoter
Bring the fabricius bursa of gained/spleen weight in wet base and chicken body weight into following formula, calculate the fabricius bursa index of respectively organizing chicken.
Fabricius bursa index=fabricius bursa weight in wet base (mg)/body weight (g)
Index and spleen index=spleen weight in wet base (mg)/body weight (g)
Acquired results SPSS statistical software analyzes, and the results are shown in Table 2-3.
The impact (mg/g) of table 2 oral liquid chicken bursa of the present invention index
The impact (mg/g) of table 3 oral liquid chicken spleen of the present invention index
Annotate: in same column data, indicate identical shoulder mark and represent that difference is not remarkable.
As can be seen from Table 2,7d, 14d after medication, 2 groups of the Chinese medicine of feeding, the fabricius bursa index is compared with matched group, significant difference (P＜0.05), and the chicken group fabricius bursa index of the prepared oral liquid of the embodiment of the present invention of feeding is apparently higher than Radix codonopsis and Radix Astragali matched group (P＜0.05).21d after medication, the prepared oral liquid group of the embodiment of the present invention still is significantly higher than Radix codonopsis and Radix Astragali matched group and blank group (P＜0.05), but rear two groups of zero differences.28d after medication, each group difference disappears.Illustrate that this prescription can promote the growth of chicken bursa before 28d, after 35d, this effect weakens.
The result of table 3 shows, the Chinese medicine of feeding is respectively organized chicken group's spleen and matched group without obvious difference, illustrate Chinese medicine of the present invention to the generation of spleen without obvious facilitation, its effect to immune organ is mainly manifested in central immune organ.
(2) oral liquid of method preparation of the present invention is on the lymphopoietic impact of chicken
After ND two exempts from 7d, 14d, 21d, 28d days, randomly draw 6 chickens for every group, the aseptic anticoagulation 1-2ml that takes adds in the centrifuge tube that fills lymphocyte separation medium, and the centrifugal 15min of 2000r/min separates lymphocyte.With the resuspended lymphocyte of culture fluid, through Trypan Blue living cell counting number＞95%, adjusting cell concentration is 1 * 10
7/ ml is in 37 ℃, saturated humidity and 5%CO
2Incubator in cultivate.The MTT staining is measured, OD
570The nm place measures the absorbance respectively organize cell, analyzes with the SPSS statistical software, the results are shown in Table 4.
The table 4 lymphopoietic impact of chicken after each Chinese prescription of feeding
Annotate: in same column data, indicate identical shoulder mark and represent that difference is not remarkable.
Experimental result shows, in whole experimental period, the lymphopoiesis that the Chinese medicine of feeding is respectively organized the chicken group significantly is better than blank group (P＜0.05), and the chicken group of the Chinese medicine oral liquid of the present invention of feeding is particularly remarkable, and than Radix Codonopsis, Radix Astragali matched group significant difference (P＜0.05).
(3) prescription of the present invention on the chicken vaccine immunity after the impact that produces of newcastle epidemic disease antibody
1 120 of age in days Avain chickens are divided into 3 groups, 40 every group.7 ages in days begin, and carry out administration by the scheme of upper table 1.7 ages in days and 18 ages in days, newcastle disease VI vaccine collunarium, eye dripping.7d, 14d, 21d, 28d after the 2nd immunity, blood sampling, separation of serum, HI test determination antibody IgY against chicken Newcastle Disease is tired.
The impact (log2) on the antibody IgY against chicken Newcastle Disease level of table 5, Chinese medicine and composition thereof
Annotate: in same column data, indicate identical shoulder mark and represent that difference is not remarkable.
Result shows, the chicken group is respectively organized in administration, after the immunity of secondary newcastle disease vaccine 7d, 14d, 21d, 28d antibody horizontal all higher than the blank group, significant difference (P＜0.05).The chicken group of the Chinese medicine oral liquid of the present invention of feeding, the newcastle epidemic disease antibody level is higher than the Radix codonopsis and Radix Astragali matched group, but both without significant difference (P＞0.05).Illustrate that prescription of the present invention and the Radix Astragali, Radix Codonopsis injection all can promote the generation of antibody IgY against chicken Newcastle Disease, and prescription of the present invention provides immune effect more remarkable than the Radix Astragali, Radix Codonopsis injection.
The impact of embodiment 3 compound Chinese medicinal preparation of the present invention on the pig immunologic function
Adopt the herbal mixture injection of the embodiment of the present invention 1 preparation example 7 gained, measure herbal mixture oral liquid of the present invention to the impact of pig immunologic function.
(1). the impact of immunostimulant of the present invention on Piglet Development and Incidence of Diarrhea
120 of the birth piglets of 1 age in days that approach term, every sow is fixed 10 piglets, and many persons entrust one's child to the care of sb..Be divided at random A, 2 groups of B, 40 every group, and by a label.A, organize in the 3rd day, 7d, 21d by following dosage regimen injection Chinese medicine injection, 0.2mg/kg body weight.Respectively weigh once before on-test, during 28 age in days, observe swinery every day, record the piglet of diarrhea, the B group is blank.The results are shown in Table 7 with table 8.
Table 6, the concrete grouping of test and processing
The impact (n=40) that table 7, immunostimulant of the present invention occur piglet diarrhea
As can be seen from Table 7, use the piglet group Incidence of Diarrhea of injection of the present invention all lower than matched group.Through X 2 test, use the swinery Incidence of Diarrhea of prescription of the present invention significantly lower than matched group (0.01＜P＜0.05).
The impact (n=40) on 28 age in days weight gain of piglets of table 8, immunostimulant of the present invention
Annotate: in same column data, indicate identical shoulder mark and represent that difference is not remarkable.
As can be seen from Table 8, in the situation that initial body weight zero difference, when using 2 group 28 ages in days of Chinese medicine, body weight is higher than blank group, significant difference.Herbal mixture injection effect of the present invention significantly is better than the Radix codonopsis and Radix Astragali matched group.
(2) impact of prescription of the present invention on swine Fever Vaccine immune antibody level
30 of Chang-Bai piglets are divided into 3 groups at random.Each is organized pig and carry out the hog cholera lapinised virus vaccine immunity inoculation when 21 age in days, intramuscular injection, 3 parts of every pig.The A group gives injection of the present invention, 0.2ml/kg body weight in immunity.The B group is the Radix codonopsis and Radix Astragali matched group, gives Radix codonopsis and Radix Astragali injection, 0.2ml/kg body weight in the time of immunity.The C group is matched group, normal immunity, not administration.14d, 30d after immunity, each group is randomly drawed 8, and vena cava anterior blood sampling, separation of serum are blocked the ELLSA test by serum, measure the titre of hog cholera antibody.
(American I DEXX produces, lot number: 99-43220S671) measure the blocking-up rate of hog cholera antibody, by specification operates, and with each hole OD value after the microplate reader assaying reaction, calculates the blocking-up rate to use serum blocking-up ELISA detection kit.
Blocking-up rate (%)=(negative control OD
450Meansigma methods-sample OD
450Meansigma methods)/negative control OD
450Meansigma methods * 100%;
The result judgement is as follows: test sample blocking-up rate 〉=40% is judged to the positive, and≤30% is judged to feminine gender, and the blocking-up rate is suspicious between 30%～40%.
Table 8, respectively organize antibody horizontal (%) after the immunity of swinery swine Fever Vaccine
Annotate: in same column data, indicate identical shoulder mark and represent that difference is not remarkable.
As can be seen from Table 10, in hog cholera immune, the compositions swinery of injection embodiment of the present invention preparation, in the rear 14d blood of immunity, the hog cholera antibody level is higher than blank group, significant difference.During 30d, in the situation that do not carry out secondary immunity, antibody horizontal descends, and Chinese drug-treated group is still higher than the blank group, and difference is not remarkable.
3. conclusion
Compound Chinese medicinal preparation of the present invention was used 7 days during 7 age in days continuously, can promote the growth of chicken central immune organ fabricius bursa; Blood lymphocytes propagation to chicken has obvious facilitation; Inject simultaneously with vaccine, can improve the level of antibody IgY against chicken Newcastle Disease in blood.Carry out intramuscular injection in 3 days, 7 days, 21 days piglet, can promote the growth promoter of piglet, obviously reduce the incidence rate of piglet diarrhea; Inject in immunity, improved the level of hog cholera antibody, strengthened the protection to swine fever.
The above is only preferred embodiment of the present invention, and is in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, is equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (10)
1. a compound Chinese medicinal preparation that strengthens livestock immunity, is characterized in that, consists of the following composition: Radix Astragali 1-5 part, Radix Codonopsis 1-5 part, Fructus Ligustri Lucidi 1-5 part.
2. compound Chinese medicinal preparation according to claim 1, is characterized in that, consists of the following composition: Radix Astragali 2-3 part, Radix Codonopsis 2-3 part, Fructus Ligustri Lucidi 2-3 part.
3. compound Chinese medicinal preparation according to claim 1 and 2, is characterized in that, described compound Chinese medicinal preparation is Chinese powder medicine, oral liquid formulation, granule dosage form or injection dosage form.
4. compound Chinese medicinal preparation according to claim 3, is characterized in that, in described oral liquid or injection dosage form, the content of the Radix Astragali comes in astragaloside, should be greater than 0.2mg/ml.
5. the preparation method as the described compound Chinese medicinal preparation of claim 1-4 any one, is characterized in that, comprises the following steps:
1) get the Radix Astragali, Fructus Ligustri Lucidi, add the ethanol 6-10 of 65-80% doubly to measure by medical material weight, reflux, extract, 4h, merge extractive liquid,, 10,000-20, the 000r/min high speed centrifugation is removed impurity, and concentrating under reduced pressure gets the Radix Astragali, Fructus Ligustri Lucidi extracting solution;
2) get Radix Codonopsis, add 8-12 times of water gaging by medical material weight, vacuum back-flow extracts 4h, extracts to concentrate simultaneously, extract and finish, merge extractive liquid,, 10,000-20, the 000r/min high speed centrifugation is removed impurity, continues to be evaporated to relative density 1.10-1.25, gets the Radix Codonopsis extracting solution;
3) merge the Radix Astragali, Fructus Ligustri Lucidi extracting solution and Radix Codonopsis extracting solution, extracting solution is concentrated into the clear paste that every ml contains primary crude drug 1-1.5g, adding 1mol/LNaOH to regulate pH value is 5.0-7.0, namely gets compound Chinese medicine extract;
4) get step 3) compound Chinese medicine extract that makes, preparation oral liquid formulation, granule dosage form or injection dosage form.
6. require described preparation method according to right 5, it is characterized in that, the step 4 of described preparation oral liquid formulation) be: get step 3) compound Chinese medicine extract that makes, be diluted with water to every milliliter and contain primary crude drug 1.2-1.5g, filter, fill, 115 ℃ of high pressure 30min sterilize, and namely get the herbal mixture oral liquid formulation; The step 4 of described preparation granule dosage form) be: get step 3) compound Chinese medicine extract that makes, 40-60 ℃ is concentrated into the thick paste that relative density is 1.30-1.35, add sucrose and dextrin, cream is heavy, the part by weight of sucrose, dextrin is 1: 2: 1, granulation carries out namely getting herbal mixture granule dosage form after drying; The step 4 of described preparation granule dosage form) be: get step 3) compound Chinese medicine extract that makes, be diluted with water to every milliliter and contain primary crude drug 1-1.2g, add the injection charcoal by the 0.5-1% volume ratio, stir 30-60min, filter, remove impurity.Filtrate injects water to scale with aperture 0.45 μ m filter membrane fine straining, regulates pH value, fill, and sterilization namely gets herbal mixture injection dosage form.
7. the application of the described compound Chinese medicinal preparation of claim 1-4 any one aspect the enhancing livestock immunity.
8. application according to claim 10, is characterized in that, the described immune effect that strengthens fowl epidemic disease of domestic animals Seedling that is applied as.
9. application according to claim 10, is characterized in that, the described immune effect that strengthens ND Vaccine that is applied as.
10. application according to claim 10, is characterized in that, the described immune effect that strengthens swine Fever Vaccine that is applied as.
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| CN104274511A (en) * | 2013-07-08 | 2015-01-14 | 洛阳惠中兽药有限公司 | Traditional Chinese medicinal composition for veterinary use and application thereof |
| CN104666399A (en) * | 2013-12-02 | 2015-06-03 | 洛阳惠中兽药有限公司 | Traditional Chinese medicine composition and preparation method and applications thereof |
| CN104800276A (en) * | 2015-05-14 | 2015-07-29 | 胡少平 | Veterinary codonopsis pilosula atractylode and astragalus Chinese medicine preparation |
| CN104800293A (en) * | 2015-05-14 | 2015-07-29 | 胡少平 | Veterinary codonopsis pilosula and astragalus immunity improving Chinese medicine preparation |
| CN107753574A (en) * | 2016-08-22 | 2018-03-06 | 北京大伟嘉生物技术股份有限公司 | A kind of herbal mixture fermentate for being used to improve chicken immune effect of vaccine |
| CN109674748A (en) * | 2019-02-15 | 2019-04-26 | 大连三仪动物药品有限公司 | A kind of traditional Chinese medicine oral liquid and its application enhancing newcastle disease vaccine immune effect |
| CN110478392A (en) * | 2019-08-02 | 2019-11-22 | 江苏康缘药业股份有限公司 | A kind of composition and preparation method thereof with strengthen immunity function |
| CN114053334A (en) * | 2021-11-04 | 2022-02-18 | 湖南东安凤康农业技术开发有限公司 | Traditional Chinese medicine composition for protecting chicken oviduct and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103520201A (en) * | 2013-07-08 | 2014-01-22 | 南京农业大学 | Chinese medicinal polysaccharide compound with function of resisting new-castle disease viruses |
| CN104274511A (en) * | 2013-07-08 | 2015-01-14 | 洛阳惠中兽药有限公司 | Traditional Chinese medicinal composition for veterinary use and application thereof |
| CN104666399A (en) * | 2013-12-02 | 2015-06-03 | 洛阳惠中兽药有限公司 | Traditional Chinese medicine composition and preparation method and applications thereof |
| CN104800276A (en) * | 2015-05-14 | 2015-07-29 | 胡少平 | Veterinary codonopsis pilosula atractylode and astragalus Chinese medicine preparation |
| CN104800293A (en) * | 2015-05-14 | 2015-07-29 | 胡少平 | Veterinary codonopsis pilosula and astragalus immunity improving Chinese medicine preparation |
| CN107753574A (en) * | 2016-08-22 | 2018-03-06 | 北京大伟嘉生物技术股份有限公司 | A kind of herbal mixture fermentate for being used to improve chicken immune effect of vaccine |
| CN109674748A (en) * | 2019-02-15 | 2019-04-26 | 大连三仪动物药品有限公司 | A kind of traditional Chinese medicine oral liquid and its application enhancing newcastle disease vaccine immune effect |
| CN110478392A (en) * | 2019-08-02 | 2019-11-22 | 江苏康缘药业股份有限公司 | A kind of composition and preparation method thereof with strengthen immunity function |
| CN114053334A (en) * | 2021-11-04 | 2022-02-18 | 湖南东安凤康农业技术开发有限公司 | Traditional Chinese medicine composition for protecting chicken oviduct and preparation method thereof |
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