CN106924352B - Application of traditional Chinese medicine composition - Google Patents
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- CN106924352B CN106924352B CN201511016112.7A CN201511016112A CN106924352B CN 106924352 B CN106924352 B CN 106924352B CN 201511016112 A CN201511016112 A CN 201511016112A CN 106924352 B CN106924352 B CN 106924352B
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Classifications
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/284—Atractylodes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
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- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides an application of a traditional Chinese medicine composition of astragalus mongholicus six-first decoction in preparation of a medicine for improving poultry immunity, wherein the raw materials of the traditional Chinese medicine composition of the astragalus mongholicus six-first decoction comprise 4-8 parts of astragalus mongholicus, 1 part of liquorice, 2-4 parts of bighead atractylodes rhizome and 2-4 parts of white paeony root. The invention also provides application of the traditional Chinese medicine composition in preparing a medicine for improving the production performance of poultry. The traditional Chinese medicine composition can effectively improve the immunity of poultry, can improve the immune effect of the vaccine and effectively improve the antibody level when being matched with the vaccine, and can also effectively reduce the material-weight ratio, reduce the morbidity and mortality and improve the production performance.
Description
Technical Field
The invention relates to a new application of a traditional Chinese medicine composition, and belongs to the field of veterinary medicines.
Background
Epidemiological investigation shows that the immunosuppressive virus infection in chicken flocks in China generally exists, common diseases mainly comprise Marek's disease, avian leukemia, infectious bursal disease, infectious laryngotracheitis, newcastle disease, infectious anemia and the like, double infection and multiple infection of the immunosuppressive virus also have certain universality, and the avian viral immunosuppressive disease is an important infectious disease which currently troubles poultry industry. In addition, the long-term use of antibiotics in large quantities leads to the increase of drug-resistant strains, the difficulty in controlling diseases and the rapid decline of the immunologic function of the poultry. Due to the existence of the immunosuppression state, the vaccine has poor immune effect or fails to immunize, thereby increasing the morbidity and treatment difficulty of poultry and causing great economic loss to the poultry industry.
"early diagnosis, effective prevention, decontamination and elimination" is the fundamental principle for the prevention of immunosuppressive diseases. However, at present, in the process of raising chickens, antibiotics and functional chemical additives with low dosage are mainly added into the feed as a method for preventing diseases and promoting growth. The long-term administration of antibiotics by chickens can cause drug residues in vivo and cause food safety problems; on the other hand, the long-term addition of antibiotics to feed is a main cause of the increase of bacterial drug resistance, and indirectly influences the treatment effect of the antibiotics for human.
The Chinese herbal medicine immunopotentiator not only can enhance the immune function of the organism, but also can improve the productivity, has the functions of nutrition and non-nutrition, has the advantages of rich resources, comprehensive functions, no toxic or side effect and no residue, and plays an important role in enhancing the immune effect of the organism and improving the production performance. Therefore, there is a need to develop a Chinese medicinal composition for enhancing chicken immunity.
In the prior art, a traditional Chinese medicine consisting of astragalus, liquorice, bighead atractylodes rhizome and white paeony root has the functions of tonifying qi and strengthening exterior. Formula of WUJIU HUANGQILIUYI decoction (Renzhai direct finger), which is prepared from radix astragali (preparata) 6 QI, radix Glycyrrhizae (preparata) 1 QI, rhizoma Atractylodis Macrocephalae 3 QI, and radix Paeoniae alba 3 QI, has effects of invigorating qi and consolidating superficial resistance, and can be used for spontaneous perspiration due to asthenia. However, this drug is not used clinically in veterinary medicine.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a new application of a traditional Chinese medicine composition, which is applied to veterinary clinic to improve the immunity and the production performance of poultry.
One aspect of the invention is to provide an application of the astragalus root six-one decoction traditional Chinese medicine composition in preparing a medicine for improving the immunity of poultry.
As an implementation mode of the invention, the raw materials of the astragalus decoction-six traditional Chinese medicine composition comprise 4-8 parts of astragalus, 1 part of liquorice, 2-4 parts of bighead atractylodes rhizome and 2-4 parts of white paeony root.
As an implementation mode of the invention, the raw materials of the astragalus root-six-one decoction traditional Chinese medicine composition comprise 6 parts of astragalus root, 1 part of liquorice, 3 parts of bighead atractylodes rhizome and 3 parts of white paeony root.
As an embodiment of the invention, the astragalus decoction-six traditional Chinese medicine composition is prepared by extracting the raw materials with water.
As an embodiment of the invention, the formulation of the astragalus decoction-six traditional Chinese medicine composition comprises granules, oral liquid and powder.
As an embodiment of the present invention, the application is the co-administration of the Chinese medicinal composition and a vaccine.
One aspect of the invention is to provide application of the astragalus root six-one decoction traditional Chinese medicine composition in preparing a medicine for improving the production performance of poultry.
As an implementation mode of the invention, the raw materials of the astragalus decoction-six traditional Chinese medicine composition comprise 4-8 parts of astragalus, 1 part of liquorice, 2-4 parts of bighead atractylodes rhizome and 2-4 parts of white paeony root.
As an implementation mode of the invention, the raw materials of the astragalus root-six-one decoction traditional Chinese medicine composition comprise 6 parts of astragalus root, 1 part of liquorice, 3 parts of bighead atractylodes rhizome and 3 parts of white paeony root.
As an embodiment of the invention, the astragalus decoction-six traditional Chinese medicine composition is prepared by extracting the raw materials with water.
As an embodiment of the invention, the formulation of the astragalus decoction-six traditional Chinese medicine composition comprises granules, oral liquid and powder.
The traditional Chinese medicine composition prepared according to the astragalus mongholicus six-one decoction formula is used in veterinary clinic, can effectively improve the immunity of poultry, can improve the immune effect of a vaccine and effectively improve the antibody level when being used in combination with the vaccine, can effectively reduce the occurrence of diseases, and improves the economic benefit; the material-weight ratio can be effectively reduced, the morbidity and the mortality are reduced, and the production performance is improved.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. These examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
The sources and lot numbers of the materials and equipment used in the invention are as follows:
astragalus decoction pieces, place of production: inner cover, batch number: 20130911, available from Qianfo pharmaceutical Co., Ltd, Henan;
licorice decoction pieces, place of production: inner cover, batch number: 20131001, available from Qianfo pharmaceutical Co., Ltd, Henan;
atractylodes decoction pieces, producing area: zhejiang, lot number: 20130525, available from Qianfo pharmaceutical Co., Ltd, Henan;
white peony root decoction pieces, production area: henan, batch number: 20130509, available from Qianfo pharmaceutical Co., Ltd, Henan;
sucrose, food grade, lot number: 20130801, available from Guangxi Tiandong county sugar manufacturing, LLC;
tween 80, pharmaceutical grade, batch number: 20130601, available from Kyojeger chemical technology, Inc., Guangzhou;
potassium sorbate, pharmaceutical grade, batch number: 20120503, available from Zhengzhou Junkai chemical products Co., Ltd;
the electric heating jacket is purchased from the Limited liability company of the instrument for Ongseihua;
a rotary evaporator purchased from consolidated yunhua instruments, llc;
centrifuge, available from sigma, germany;
vacuum drying cabinet, model DZF-6050, purchased from Shanghai Boxun industries, Inc.
The following examples further illustrate embodiments of the present invention:
example 1 preparation of a Chinese medicinal composition of six-in-one decoction of Astragalus
(1) Preparation of astragalus root six-in-one decoction traditional Chinese medicine composition granules
1) Weighing 600g of astragalus mongholicus, 100g of liquorice, 300g of bighead atractylodes rhizome and 300g of white paeony root, adding 8 times of water, heating, refluxing and extracting for 3 times, each time for 2 hours, filtering for several times, combining filtrates, concentrating under reduced pressure to relative density of 1.10(70 ℃), cooling to room temperature, centrifuging, concentrating supernatant under reduced pressure to relative density of 1.20(70 ℃), and obtaining traditional Chinese medicine extract.
2) Taking the Chinese medicinal extract, adding 720g of sucrose powder, vacuum drying at 60 ℃ for 24h, pulverizing, granulating with 90% ethanol as wetting agent, drying at 50 ℃ for 2h, and grading to obtain granule.
(2) Preparation of astragalus root six-one decoction traditional Chinese medicine composition oral liquid
1) Weighing 600g of astragalus mongholicus, 100g of liquorice, 300g of bighead atractylodes rhizome and 300g of white paeony root, adding 8 times of water, heating, refluxing and extracting for 3 times, each time for 2 hours, filtering for several times, combining filtrates, concentrating under reduced pressure to relative density of 1.10(70 ℃), cooling to room temperature, centrifuging, concentrating supernatant under reduced pressure to relative density of 1.20(70 ℃), and obtaining traditional Chinese medicine extract.
2) Adding water into the Chinese medicinal extract to 1200ml, adding 65.0g tween 80 and 13.0g potassium sorbate, stirring for dissolving, diluting with water to 1300ml, filtering with 0.45 μm filter membrane, bottling, plugging, and sterilizing at 100 deg.C for 30min to obtain oral liquid.
(3) Preparation of astragalus root six-one decoction traditional Chinese medicine composition powder
1) Weighing 600g of astragalus mongholicus, 100g of liquorice, 300g of bighead atractylodes rhizome and 300g of white paeony root, adding 8 times of water, heating, refluxing and extracting for 3 times, each time for 2 hours, filtering for several times, combining filtrates, concentrating under reduced pressure to relative density of 1.10(70 ℃), cooling to room temperature, centrifuging, concentrating supernatant under reduced pressure to relative density of 1.20(70 ℃), and obtaining traditional Chinese medicine extract.
2) Taking the Chinese medicinal extract, pouring into a stainless steel tray, vacuum drying at 60 deg.C for 24 hr, taking out, pulverizing, and sieving with 80 mesh sieve to obtain powder.
Example 2 pharmacodynamic test conditions for enhancing immunity
The experiment provides a basis for the clinical application of the astragalus mongholicus six-one decoction traditional Chinese medicine composition in enhancing the immune function by observing the influence of the astragalus mongholicus six-one decoction traditional Chinese medicine composition on T lymphocytes in chicken blood.
Experimental animals: the test chickens are 90 healthy AA broilers of 10 days old, and are managed by a conventional feeding method, and the feeding management conditions are consistent during the test period.
Grouping and processing experimental animals: 90 healthy AA broilers at age of 10 days were randomly divided into A, B, C three groups of 30 broilers each. Group A, the astragalus root six-one decoction traditional Chinese medicine composition granules prepared in the example 1 are given; group B administration of Astragalus polysaccharides; group C was a blank control group, not dosed. The administration and treatment methods are detailed in table 1.
Feeding management: the test chickens were only housed in groups in the isolator in the same animal laboratory. 3 test groups were routinely fed on basal diets. During the whole test period, the patient can eat and drink water freely, and the patient can be cleaned every day.
TABLE 1 animal grouping and administration method
| Group of | Quantity (only) | Using and using amount |
| A | 30 | 1.0g of water per 1L, drinking water freely, and continuously for 7 days |
| B | 30 | 0.2g of water per 1L, drinking water freely for 7 days continuously |
| C | 30 | Free drinking water without administration |
The experimental method comprises the following steps: each group was administered for 7 days, and 10 chickens per group were randomly sampled after drug withdrawal for blood sampling and E-rosette test.
And (4) judging a result: observing about 200 lymphocytes under a light microscope, wherein the cells with 3 or more sheep red blood cells adhered to the surfaces of the lymphocytes are rosette forming cells, and calculating the percentage of the rosette forming cells in the total number of the lymphocytes. Statistical processing was performed using SPSS analysis software. In particular, see the following table:
TABLE 2 percentage of rosette formation in T lymphocytes of each test group
The results show that: the difference of the forming rate of lymphocyte rosette in group A and group C is very obvious, P is less than 0.01, which shows that the astragalus root six-one decoction traditional Chinese medicine composition can obviously increase the number of T lymphocytes in chicken blood and improve the immune function of the T lymphocytes; the difference of the lymphocyte rosette formation rate of the group B and the comparison group is obvious, P is less than 0.05, which indicates that astragalus polysaccharide can also obviously increase the number of T lymphocytes in chicken blood and improve the immune function of the T lymphocytes; the difference between the forming rate of lymphocyte rosette in group A and the forming rate of lymphocyte rosette in group B is extremely obvious, and P is less than 0.01, which shows that the astragalus root six-in-one decoction traditional Chinese medicine composition can obviously increase the number of T lymphocytes in chicken blood and improve the immune function of the T lymphocytes compared with astragalus root polysaccharide.
Example 3 Experimental conditions for increasing antibody levels in combination with vaccines
The experiment mainly aims to provide a basis for the traditional Chinese medicine composition of astragalus root six-one decoction to enhance the immune function by comparing the effect of the medicine on enhancing the immunity of the newcastle disease vaccine.
Vaccine: newcastle disease virus and avian influenza virus bivalent inactivated vaccine (La Sota strain + H9 subtype, HL strain), lot No. 150633, manufacturer: praco bioengineering, Inc.
Experimental animals: 1 day old healthy SPF Roman egg male chicks are raised in an isolator, and during the test period, SPF chicken feed is fed, and free feeding and drinking are carried out.
Grouping experimental animals: feeding to 8 days of age, selecting 90 healthy chickens, randomly dividing into A, B, C test groups, each group comprising 30 chickens, each numbered, and feeding in 3 isolators according to the group.
The experimental method comprises the following steps: at 9 days of age, randomly extracting 5 chickens from each group, collecting non-anticoagulated blood, and determining the antibody titer of Newcastle disease by reference to the GB/T16550-2008 standard hemagglutination inhibition method. At 10 days of age, each group was immunized by subcutaneous injection of newcastle disease virus and avian influenza virus bivalent inactivated vaccine (La Sota strain + H9 subtype, HL strain) into the neck and back at 0.1 ml/feather. The next day of immunization, group a was administered the astragalus siyitang chinese medicinal composition granules prepared in example 1; group B administration of Astragalus polysaccharides; group C was a blank control group, not dosed. The administration and treatment methods are detailed in table 3. In each group, 10 chickens were randomly selected 28 days after immunization, each chicken was bled with 1ml of blood, and serum was separated to determine the newcastle disease antibody titer.
TABLE 3 animal grouping and dosing methods
| Group of | Quantity (only) | Using and using amount |
| A | 30 | 1.0g of water per 1L, drinking water freely, and continuously for 7 days |
| B | 30 | 0.2g of water per 1L, drinking water freely for 7 days continuously |
| C | 30 | Free drinking water without administration |
Data processing: the significance of the differences of the groups was compared by analysis with SPSS software.
The experimental results are as follows:
TABLE 4 dynamic changes in the antibody titers against Newcastle disease for each group
The results show that: before immunization, the Newcastle disease antibody titer of each group of chickens is 0; at 28 days after immunization, the antibody titers in group A were significantly different from those in group C (P <0.01), those in group B (P <0.05), and those in group A (P > 0.05). The astragalus root six-one decoction traditional Chinese medicine composition has a strong immunity enhancing effect, and can improve the immune response of a vaccine and the antibody level.
Example 4 test conditions for improving broiler productivity
Experimental animals: 10077 new yellow feather broilers are tested in a certain culture farm in Luoyang Ischuan.
The experimental method comprises the following steps: 10077 yellow-feathered broilers of 7 days old are divided into a group A and a group B, 5065 in the group A are given the astragalus six-one decoction traditional Chinese medicine composition granules prepared in the example 1, and 5012 in the group B are blank control groups and are not given. The 2 groups were all managed according to the normal procedure of the farm, and the administration and treatment methods are detailed in table 5. At 7, 24, 45, 58 days of age (slaughter), 500 chickens per group were randomly picked and weighed, and the average weight was calculated. And respectively calculating the daily average weight gain, daily average feed intake, total feed consumption and total weight gain of 3 time periods of 8-24 days old, 25-45 days old and 46-58 days old, and calculating the feed-weight ratio. During the test period, the number of the sick and dead chickens in each group of chickens is observed and recorded, and the morbidity and the mortality in the whole test period are calculated.
TABLE 5 animal grouping and dosing methods
| Group of | Quantity (only) | Using and using amount |
| A | 5065 | 1.0g of water per 1L, free drinking water |
| B | 5012 | Free drinking water without administration |
Data processing: the significance of the differences of the groups was compared by analysis with SPSS software.
The experimental results are as follows:
TABLE 6 results of the Performance measurements of chickens during the test period
As can be seen from Table 6, compared with the blank control group, the daily average weight gain of the administration groups at 3 days of 8-24d, 25-45d and 46-58 d is respectively improved by 4.40%, 2.32% and 5.86%, and the material weight ratio of the administration groups at 3 days is respectively reduced by 2.34%, 4.90% and 8.60%. In the whole test period, compared with a blank control group, the daily gain of the administration group is increased by 4.11%, and the material-weight ratio is reduced by 5.88%. The astragalus root six-in-one decoction traditional Chinese medicine composition has the effects of improving the weight gain rate of chicken flocks, reducing the feed weight ratio and improving the feed reward.
TABLE 7 morbidity and mortality in groups of chickens during the test period
As can be seen from table 7, the morbidity and mortality of the blank control group were 7.88% and 2.89% respectively, while the morbidity and mortality of the administration group under the same feeding and management conditions were 4.78% and 1.50% respectively, which were reduced by 3.10% and 1.39% respectively. The astragalus root-six-one decoction traditional Chinese medicine composition can reduce the disease rate and the death rate of chickens and improve the economic benefit of breeding.
The preferred embodiments of the present invention have been fully described above, but various substitutions and modifications may be made thereto. The scope of the invention should, therefore, be determined not with reference to the above description, but instead should be determined with reference to the appended claims along with their full scope of equivalents. Any feature, whether preferred or not, may be combined with any other feature, whether preferred or not. The claims hereof are not to be read as having method + functional limitations unless such limitations are expressly recited in a claim by the term "method of …".
Claims (3)
1. The application of the astragalus mongholicus six-first decoction traditional Chinese medicine composition in preparing a medicine for improving the weight gain rate of chicken flocks and reducing the material weight ratio is characterized in that the astragalus mongholicus six-first decoction traditional Chinese medicine composition is prepared from 4-8 parts of astragalus mongholicus, 1 part of liquorice, 2-4 parts of bighead atractylodes rhizome and 2-4 parts of white paeony root.
2. The use of claim 1, wherein the raw materials of the astragalus root-six-one decoction traditional Chinese medicine composition are 6 parts of astragalus root, 1 part of liquorice, 3 parts of bighead atractylodes rhizome and 3 parts of white peony root.
3. The use of claim 1, wherein the astragalus decoction-six traditional Chinese medicine composition is in the dosage forms of granules, oral liquid and powder.
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