Disclosure of Invention
In order to solve the technical problems, the invention aims to provide a safe and efficient traditional Chinese medicine composition for poultry.
The invention aims at realizing the following technical scheme:
the invention provides a traditional Chinese medicine composition for poultry, which contains active ingredients, wherein the active ingredients comprise: astragalus root, pilose asiabell root, dwarf lilyturf tuber, schisandra fruit, cowpea, chinese fevervine herb, sweet wormwood herb and manninoligosaccharide.
Further, the active ingredients are prepared from 20-70 parts by weight of astragalus mongholicus, 10-50 parts by weight of codonopsis pilosula, 10-40 parts by weight of radix ophiopogonis, 5-25 parts by weight of schisandra chinensis, 5-20 parts by weight of cowpea, 10-30 parts by weight of herba paederiae, 8-26 parts by weight of sweet wormwood and 70-100 parts by weight of mannooligosaccharide.
Further, the active ingredients are prepared from, by weight, 30-60 parts of astragalus mongholicus, 20-40 parts of codonopsis pilosula, 20-35 parts of radix ophiopogonis, 10-20 parts of schisandra chinensis, 8-16 parts of cowpea, 15-25 parts of Chinese fevervine, 12-22 parts of sweet wormwood herb and 80-90 parts of mannooligosaccharide.
Further, the active ingredients are prepared from 50 parts by weight of astragalus membranaceus, 30 parts by weight of codonopsis pilosula, 28 parts by weight of dwarf lilyturf tuber, 16 parts by weight of schisandra chinensis, 15 parts by weight of cowpea, 20 parts by weight of Chinese fevervine herb, 18 parts by weight of sweet wormwood herb and 85 parts by weight of mannooligosaccharide.
The invention also provides a preparation method of the traditional Chinese medicine composition for poultry, which comprises the following steps:
(1) The raw materials are respectively weighed according to the weight ratio.
(2) The preparation method comprises coarse pulverizing the above Chinese medicinal materials except for mannooligosaccharide, decocting with 10-12 times of water for 2-3 times each for 1-2 hr, filtering, mixing filtrates, and concentrating the filtrate under reduced pressure to obtain concentrated extract (1 g is equivalent to 3g of crude drug).
(3) Adding weighed mannooligosaccharide into the concentrated extract obtained in the step 2, uniformly mixing, adding sucrose and dextrin (the ratio of sucrose to dextrin is 1:1), uniformly mixing, and preparing into particles.
The traditional Chinese medicine composition for poultry can be used for enhancing the immunity of organisms and improving the disease resistance of poultry.
In particular to a medicine for enhancing the immunity of poultry and improving the disease resistance by preparing the traditional Chinese medicine composition for poultry. Preferably, the avian is an avian that has been vaccinated; preferably, the vaccinations are newcastle disease vaccines and avian influenza vaccines.
The invention also provides premix, concentrate and batch containing the traditional Chinese medicine composition for poultry.
Compared with the prior art, the traditional Chinese medicine composition has the following beneficial effects:
the traditional Chinese medicine composition is prepared by repeatedly verifying and practicing a large number of experiments according to the dialectical treatment theory of Chinese veterinary prescriptions, combines the immune mechanism of animal organisms and the reasons and clinical symptoms of partial diseases, and has obvious effects of improving the immunity and disease resistance of the organisms under the guidance of the dialectical treatment theory of traditional Chinese medicine through the precise prescription obtained by verifying a large number of clinical effects.
The occurrence of any disease is considered by the traditional Chinese medicine to be the result of struggle between the healthy qi and the pathogenic qi under certain conditions. The healthy qi is stored in the body, the pathogenic factors can not be dried, the pathogenic factors are accumulated, the qi is deficient, the disease is generated and developed, the strength contrast and the overgrowth and decline change between the healthy qi and the pathogenic factors exist all the time, and the disease development and the prognosis are directly affected. The defensive power of healthy qi is particularly characterized in the aspects of resisting invasion of exogenous evil, expelling evil, repairing and regulating ability, maintaining coordination of viscera and meridian functions and the like. The invasion of pathogenic qi mainly leads to physiological dysfunction, damage to the viscera and tissues, and changes in the body constitution. When healthy qi is greater than pathogenic qi, the body is in a healthy state, otherwise in a pathological state. So that the immunity and disease resistance of the organism are improved, and the healthy energy of the organism is improved. The therapeutic principle is to strengthen the body resistance and eliminate pathogenic factors, and the strengthening body resistance and eliminating pathogenic factors should be closely connected. Strengthening body resistance to enhance the body resistance of the livestock to defeat pathogenic factors; the pathogenic factors are eliminated to maintain healthy qi, that is, eliminate pathogenic factors, so that qi and blood of viscera of the livestock are recovered to be normal. The healthy qi of the middle-jiao veterinarian includes the functional activities of viscera, tissues and organs of the organism, the ability of the organism to resist environmental stress and the organism's resistance to pathogenic factors. To achieve what the veterinarian says "strengthening" one aspect, it is necessary to enhance "functional activities of the viscera, tissues and organs"; in order to enhance the functional activities of viscera, tissues and organs, the method of invigorating yang qi is needed. Such as spleen qi and kidney yang.
In the invention, astragalus and radix codonopsis can promote the production of body fluid and recover pulse, and greatly tonify primordial qi to be used as monarch drug; radix Ophiopogonis has effects of nourishing yin, promoting fluid production, and supplementing qi, and is used as ministerial drug; wu Wei Zi astringes yin to arrest sweating, engenders liquid to replenish qi, so it is good at astringing and gathering true qi for dissipation. The cowpea has the effects of dispelling wind and relieving cough, and resolving food stagnation, and is taken as an adjuvant drug; herba Paederiae has effects of dispelling pathogenic wind, promoting blood circulation, relieving pain, removing toxic substance, resolving food stagnation, eliminating dampness, relieving swelling, tonifying consumptive disease, regulating primordial qi of spleen and stomach, and reducing heat stress. Herba Artemisiae Annuae has effects of clearing deficiency heat, cooling blood, relieving summer heat, preventing malaria, increasing lymphocyte transformation rate, promoting organism cell immunity, and inhibiting bacteria and killing parasite. The medicines are combined together, and have the effects of nourishing yin, promoting the production of body fluid, tonifying qi, activating blood, promoting digestion, removing food stagnation and enhancing immunity.
According to the invention, the mannooligosaccharide is added on the basis of the traditional Chinese medicine composition, and experiments prove that the antibody level of the chick vaccinated with the newcastle disease vaccine can be further improved by combining the mannooligosaccharide and the traditional Chinese medicine composition. Meanwhile, compared with the existing products, the traditional Chinese medicine composition provided by the invention has excellent effects on improving the antibody level of poultry and preventing poultry diseases.
Detailed Description
The following examples are illustrative of the invention and are not intended to limit the scope of the invention.
Example 1
(1) Weighing the traditional Chinese medicinal materials with the following weight portions: 20 parts of astragalus, 10 parts of codonopsis pilosula, 10 parts of dwarf lilyturf tuber, 5 parts of schisandra chinensis, 5 parts of cowpea, 10 parts of Chinese fevervine herb, 8 parts of sweet wormwood herb and 70 parts of mannooligosaccharide.
(2) The preparation method comprises coarse pulverizing the above Chinese medicinal materials except for mannooligosaccharide, decocting with 10-12 times of water for 2-3 times each for 1-2 hr, filtering, mixing filtrates, and concentrating the filtrate under reduced pressure to obtain concentrated extract (1 g is equivalent to 3g of crude drug).
(3) Adding weighed mannooligosaccharide into the concentrated extract obtained in the step 2, uniformly mixing, adding sucrose and dextrin (the ratio of sucrose to dextrin is 1:1), uniformly mixing, and preparing into particles.
Example 2
(1) Weighing the traditional Chinese medicinal materials with the following weight portions: 70 parts of astragalus, 50 parts of codonopsis pilosula, 40 parts of dwarf lilyturf tuber, 25 parts of schisandra chinensis, 20 parts of cowpea, 30 parts of Chinese fevervine herb, 26 parts of sweet wormwood herb and 100 parts of mannooligosaccharide
(2) The preparation method comprises coarse pulverizing the above Chinese medicinal materials except for mannooligosaccharide, decocting with 10-12 times of water for 2-3 times each for 1-2 hr, filtering, mixing filtrates, and concentrating the filtrate under reduced pressure to obtain concentrated extract (1 g is equivalent to 3g of crude drug).
(3) Adding weighed mannooligosaccharide into the concentrated extract obtained in the step 2, uniformly mixing, adding sucrose and dextrin (the ratio of sucrose to dextrin is 1:1), uniformly mixing, and preparing into particles.
Example 3
(1) Weighing the traditional Chinese medicinal materials with the following weight portions: 30 parts of astragalus, 20 parts of codonopsis pilosula, 20 parts of dwarf lilyturf tuber, 10 parts of schisandra chinensis, 8 parts of cowpea, 15 parts of Chinese fevervine herb, 12 parts of sweet wormwood herb and 80 parts of mannooligosaccharide
(2) The preparation method comprises coarse pulverizing the above Chinese medicinal materials except for mannooligosaccharide, decocting with 10-12 times of water for 2-3 times each for 1-2 hr, filtering, mixing filtrates, and concentrating the filtrate under reduced pressure to obtain concentrated extract (1 g is equivalent to 3g of crude drug).
(3) Adding weighed mannooligosaccharide into the concentrated extract obtained in the step 2, uniformly mixing, adding sucrose and dextrin (the ratio of sucrose to dextrin is 1:1), uniformly mixing, and preparing into particles.
Example 4
(1) Weighing the traditional Chinese medicinal materials with the following weight portions: 60 parts of astragalus, 40 parts of codonopsis pilosula, 35 parts of dwarf lilyturf tuber, 20 parts of schisandra chinensis, 16 parts of cowpea, 25 parts of Chinese fevervine herb, 22 parts of sweet wormwood herb and 90 parts of mannooligosaccharide
(2) The preparation method comprises coarse pulverizing the above Chinese medicinal materials except for mannooligosaccharide, decocting with 10-12 times of water for 2-3 times each for 1-2 hr, filtering, mixing filtrates, and concentrating the filtrate under reduced pressure to obtain concentrated extract (1 g is equivalent to 3g of crude drug).
(3) Adding weighed mannooligosaccharide into the concentrated extract obtained in the step 2, uniformly mixing, adding sucrose and dextrin (the ratio of sucrose to dextrin is 1:1), uniformly mixing, and preparing into particles.
Example 5
(1) Weighing the traditional Chinese medicinal materials with the following weight portions: 50 parts of astragalus, 30 parts of codonopsis pilosula, 28 parts of dwarf lilyturf tuber, 16 parts of schisandra chinensis, 15 parts of cowpea, 20 parts of Chinese fevervine herb, 18 parts of sweet wormwood herb and 85 parts of mannooligosaccharide.
(2) The preparation method comprises coarse pulverizing the above Chinese medicinal materials except for mannooligosaccharide, decocting with 10-12 times of water for 2-3 times each for 1-2 hr, filtering, mixing filtrates, and concentrating the filtrate under reduced pressure to obtain concentrated extract (1 g is equivalent to 3g of crude drug).
(3) Adding weighed mannooligosaccharide into the concentrated extract obtained in the step 2, uniformly mixing, adding sucrose and dextrin (the ratio of sucrose to dextrin is 1:1), uniformly mixing, and preparing into particles (1 g of particles is equivalent to 3g of crude drug).
Comparative example 1
A Chinese herbal medicine immunopotentiator for chicken, which can improve the immunity of chicken and strengthen the disease resistance and disease prevention capability, comprises the following components in parts by weight: 40 parts of echinacea purpurea, 20 parts of astragalus, 10 parts of rhodiola rosea, 20 parts of codonopsis pilosula and 10 parts of liquorice.
The preparation method of the Chinese herbal medicine immunopotentiator for chicken, which can improve the immunity of chicken and strengthen the disease resistance and disease resistance, comprises the following steps:
(1) The raw materials are respectively weighed according to the weight ratio.
(2) Coarse pulverizing the above materials, decocting with 10-12 times of water for 2-3 times each for 1-2 hr, filtering, mixing filtrates, and concentrating the filtrate under reduced pressure to obtain concentrated extract (1 g corresponds to 3g of crude drug).
(3) Adding sucrose and dextrin (sucrose and dextrin are added in a ratio of 1:1) into the concentrated extract obtained in the step 2, uniformly mixing, and preparing into particles (1 g of particles is equivalent to 3g of crude drug).
Comparative example 2
The astragalus pulse-activating drink medicine composition, the preparation method and the feed are calculated according to parts by weight: 50 parts of astragalus, 30 parts of codonopsis pilosula, 25 parts of dwarf lilyturf tuber, 15 parts of schisandra chinensis, 30 parts of echinacea, 25 parts of maca, 20 parts of vitamin C and 20 parts of citric acid.
The medicine composition, the preparation method and the feed preparation method of the astragalus pulse-activating decoction comprise the following steps:
(1) Weighing the traditional Chinese medicine raw materials containing the traditional Chinese medicine components, coarsely crushing, adding 10 parts of water, decocting for 2 times, each time for 1h, filtering, combining the filtrates, concentrating the filtrate under reduced pressure, drying into dry paste, and crushing for standby.
(2) And (3) weighing vitamin C and citric acid respectively, and uniformly mixing with the dry paste powder obtained in the step (1) to obtain the traditional Chinese medicine (1 g of particles is equivalent to 3g of crude medicinal materials).
Comparative example 3
The astragalus pulse-activating decoction comprises the following components in parts by weight: 15 parts of astragalus, 9 parts of codonopsis pilosula, 9 parts of dwarf lilyturf tuber and 5 parts of Chinese magnoliavine fruit.
The preparation method of the astragalus pulse-activating decoction comprises the steps of weighing the medicines of the components, crushing, adding 10 times of water, decocting for 2 times, each time for 1h, filtering, combining filtrate, concentrating the filtrate under reduced pressure to obtain a concentrated extract, adding dextrin, and preparing into granules (1 g of granules is equivalent to 3g of crude drug).
Comparative example 4
50 parts of astragalus, 30 parts of codonopsis pilosula, 28 parts of dwarf lilyturf tuber, 16 parts of schisandra chinensis, 15 parts of cowpea, 20 parts of Chinese fevervine herb and 18 parts of sweet wormwood herb.
The preparation method of the comparative example 4 comprises the following steps:
(1) Weighing the above Chinese medicines, coarse pulverizing, decocting with 10-12 times of water for 2-3 times each for 1-2 hr, filtering, mixing filtrates, and concentrating the filtrate under reduced pressure to obtain concentrated extract (1 g corresponds to 3g of crude drug).
(2) Adding sucrose and dextrin (sucrose and dextrin are added in a ratio of 1:1) into the concentrated extract obtained in the step 1, uniformly mixing, and preparing into particles (1 g of particles is equivalent to 3g of crude drug).
In order to show the effect of the traditional Chinese medicine composition, the applicant further carries out clinical curative effect observation tests on the traditional Chinese medicine composition, and the beneficial effects of the traditional Chinese medicine composition are further illustrated by test examples.
Test example 1 test of the influence of the Chinese medicinal composition of the invention on the antibody level of Newcastle disease vaccine
The immune enhancement effect of the traditional Chinese medicine composition prepared by the embodiment of the invention is examined by observing the influence of the traditional Chinese medicine composition on the antibody level of the newcastle disease vaccine.
1 Material
Test drug: the traditional Chinese medicine compositions prepared in the embodiments 1, 2, 3, 4 and 5 of the invention; the medicaments prepared in comparative examples 1, 2, 3 and 4; control drug: astragalus polysaccharide extract.
The test article is NDV standard antigen and positive serum, and is used for determining the titer of NDVHI antibody.
Test animals: sea blue brown chick
Test method and grouping: 240 sea-blue brown chickens are randomly divided into 12 groups, each group is provided with 4 repeats, 5 chickens are fed on the net for flat raising according to a conventional raising method, and full-price compound feed without any medicine is fed for free feeding and drinking water. Chicks are bred to 14 days old and are immunized for the first time by using an ND vaccine through eye and nose dropping; secondary immunization was performed at 28 days of age with water. The free drinking water administration was continued for 7 days after the first and second days, respectively, and the administration doses were as shown in Table 1 below, 2 chickens were randomly taken from each of the repeated groups at 21 days old and 35 days old, and 8 chickens were collected from each group, 0.2ml was collected intravenously, and the serum was centrifuged at 1.5ml, and the ND antibody level of the chickens was detected by HI method, and the specific grouping and treatment methods were as shown in Table 2 below.
Table 1 test group and dosing conditions
2. Test results and analysis
Table 2 test results
As can be seen from table 2, the antibody level of the group of example 5 was best elevated compared to the control drug, which is the optimal prescription of the present invention. The example 5 group showed a significant increase in both the level of the average antibody at 21 st balance after the first and the level of the average antibody at 35 th balance after the second, compared with the comparative examples 1, 2 and 3, indicating that the formulation of the present invention gave a significant effect compared with the same product as the conventional base formulation. Example 5 the level of the balance 21 was raised from 4.25 to 5.56 and the level of the balance 35 was raised from 6.73 to 7.67 compared to comparative example 4 after the priming, and it was seen that the addition of mannooligosaccharides had a significant synergistic effect.
Test example 2 observation of the prevention Effect of the Chinese medicinal composition of the present invention on New castle disease
And 7 months in 2020, selecting a certain chicken farm in Shandong with a new castle disease history for testing. The 6000 day-old Ev chickens were randomly divided into 4 groups of 1500 chickens, each group being trial 1, trial 2, trial 3 and trial 4. The test 1 group and the test 2 group are the drug groups of the invention, the test 3 group is the test group of the comparative example 1, and the test 1 group is fed freely according to 1 kilogram of the traditional Chinese medicine composition prepared in the embodiment 5 of the invention added into each ton of feed and is continuously administered for 14 days; the test 2 groups are fed with 1 kg of the traditional Chinese medicine composition prepared in the embodiment 1 according to each ton of feed, and are fed for 14 days continuously; the test 3 group uses the medicine of the comparative example 1, and 1 kg of the medicine of the comparative example 1 is added into each ton of feed, and the feed is taken freely and continuously administered for 14 days; test 4 was a blank control group, given no drug, and the feeding conditions were substantially identical. During the test, the isolation protection and disinfection work is done. After the end of administration, the administration was observed for 40 days, and the test results are shown in Table 3.
TABLE 3 Newcastle disease prevention test results
The test results show that the traditional Chinese medicine composition prepared in the embodiment 5 and the embodiment 1 and the medicine in the comparative example 1 can effectively prevent the occurrence of the newcastle disease, the traditional Chinese medicine composition prepared in the embodiment 5 has the best effect, the morbidity is 0, the effective rate can reach 100 percent, the traditional Chinese medicine composition is the optimal prescription of the invention, the effect of preventing the newcastle disease of the medicine composition is far better than that of the medicine group in the comparative example 1, and the difference is obvious compared with the blank control group. The medicine has obvious effect of preventing chicken newcastle disease.
Test example 3 observation of the effect of the Chinese medicinal composition of the present invention on preventing avian influenza
An avian influenza prevention test was performed on a chicken farm of Jiangsu Nanjing with a history of avian influenza, and 2000 chickens were randomly divided into 4 groups of 500 chickens each. Group 1, wherein 1 kg of the traditional Chinese medicine composition prepared in the embodiment 5 of the invention is added into each ton of feed for mixed feeding and administration, and free feeding is carried out for 7 days; group 2, wherein 1 kg of the traditional Chinese medicine composition prepared in the embodiment 3 of the invention is added into each ton of feed, mixed feeding and administration are carried out, and free feeding is carried out for 7 days; group 3, wherein 1 kg of the traditional Chinese medicine composition prepared in the comparative example 4 is added into each ton of feed for mixed feeding and administration, and the feed is freely fed for 7 days; in the first group, no medicine is added into the feed. After the end of administration, the administration was observed for 40 days. The test results are shown in Table 4.
TABLE 4 test results of avian influenza prevention
Group of
|
Animal number (head)
|
Number of animals (head)
|
Incidence of disease
|
Group 1 (example 5)
|
500
|
0
|
0.00%
|
Group 2 (example 3)
|
500
|
1
|
0.200%
|
Group 3 (comparative example 4)
|
500
|
25
|
5.00%
|
Group 4 (blank)
|
500
|
89
|
17.80% |
The test results show that the traditional Chinese medicine composition of the invention in the embodiment 5 and the embodiment 3 and the medicine of the comparative example 4 have remarkable effects on preventing the avian influenza, and the protection rate of the traditional Chinese medicine composition of the invention can reach 100% and 99.80%, which is better than that of the comparative example 4, so that the traditional Chinese medicine composition has a synergistic effect between the mannooligosaccharide and the traditional Chinese medicine composition of the invention. The differences between the test 1, 2 and 3 groups and the blank control group are significant. The traditional Chinese medicine composition has a very good effect of preventing the avian influenza.
The foregoing is merely a preferred embodiment of the present invention, and it should be noted that it will be apparent to those skilled in the art that several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the scope of the invention.