CN104666399A - Traditional Chinese medicine composition and preparation method and applications thereof - Google Patents
Traditional Chinese medicine composition and preparation method and applications thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for reducing immunological stress reactions of livestock and poultry. The traditional Chinese medicine composition comprises the following raw materials: 2-10 parts of radix astragali and 1 part of manyprickle acanthopanax roots. The traditional Chinese medicine composition disclosed by the invention, after being taken by livestock and poultry before and after being immunized, can reduce immunological stress reactions of vaccines, enhance the immune effect, reduce respiratory symptoms such as snore and cough and the like occurring after poultry vaccination is performed, and improve the appetite loss, slow weight gain and productivity reduction of livestock and poultry caused by immunological stress reactions.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, with and its preparation method and application, belong to field of veterinary.
Background technology
Along with aquaculture is to while intensive, large-scale development, level and the cultivation idea of culturist also there occurs huge change, and the policy of " put prevention first, anti-overweighting is controlled " gets the nod gradually, and prevention and health care is brought into schedule day by day.The prevention of livestock and poultry mainly realizes by vaccine immunity; vaccine is as a kind of alloantigen; while stimulation body produces protection antibody; also cause body and occur stress; as within after the immunity of poultry live vaccine 2nd ~ 4 days, occurred getting rid of head, shed tears, sneeze, snore, the symptom such as cough; reaction in these symptom 5-6 days is heavier, alleviates gradually later.But the appearance of vaccine immunity stress makes immunity of organisms decline, easily cause other diseases or cause immune antibody level uneven, even causing immuning failure.The disease caused by systemic immune response the most easily occurred in poultry is produced is the respiratory symptom occurred after newcastle disease vaccine head exempts from, think that this symptom is caused by mycoplasma infection at present, if treat not in time, can be occurred together colibacillosis, increase treatment difficulty, and the primary measure solving mycoplasma secondary infection is the systemic immune response that minimizing vaccination causes.
For young animal, as chickling needs repetitious immunity before 40 ages in days, add that its immune defenses is poor, be easy to occur systemic immune response.And owing to losing the protection of maternal antibody after weaned piglet; vaccinate repeatedly easily causes baby pig diarrhea, respiratory tract side reaction; add the stimulation of injection site, the appearance of these stressors cause Piglet Development slowly, resistance declines, easily disease occurs.Therefore alleviate the objective demand that systemic immune response that vaccination causes is aquaculture development, be also the key point ensureing immune success rate.
At present in breeding production, raiser's many uses electrolysis multidimensional or glucose drinking-water for turning group, reload, Changes in weather time anti-stress and vaccination before and after and the growth promotion in later stage.Electrolysis multidimensional and glucose drinking-water stress play good mitigation to what cause because of factors such as long-distance transport, poisoning, environmental changes, but for reducing side effect that vaccination causes, to alleviate systemic immune response effect unsatisfactory.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of and can alleviate the Chinese medicine composition that veterinary vaccines inoculates the systemic immune response caused, wherein, described traditional Chinese medicine composite is Radix Astragali 2-10 part, Radix Et Caulis Acanthopanacis Senticosi 1 part.
Preferably, described traditional Chinese medicine composite comprises Radix Astragali 3-6 part, Radix Et Caulis Acanthopanacis Senticosi 1 part.Described Chinese medicine composition principle active component is total sugar and astragaloside.
Preferably, described Chinese medicine composition comprises pharmaceutically acceptable carrier further, and described carrier comprises diluent, disintegrating agent, inclusion agents, wetting agent.
Pharmaceutically acceptable carrier of the present invention can be that any Chinese medicine granules of preparing is commonly used or acceptable carrier in conventional pharmaceutical, such as: diluent (filler) includes but not limited to sucrose, dextrin, starch, lactose, mannitol, xylitol, chitosan, Bidismutose, soluble starch, Pulvis Talci or water solublity dextrin etc.; Disintegrating agent includes but not limited to starch, sodium carboxymethyl cellulose (CMS-Na), microcrystalline Cellulose (MCC), micropowder silica gel, hydroxypropyl starch, soluble starch, water solublity dextrin; Inclusion agents includes but not limited to alpha-cyclodextrin (α-CD), beta-schardinger dextrin-(β-CD) and N-LOK modified starch etc.; Wetting agent (binding agent) includes but not limited to water, ethanol, polyvinylpyrrolidone (polyvidone), hydroxypropyl cellulose, Polyethylene Glycol (PEG) etc.Above-mentioned pharmaceutically acceptable carrier can be used alone, also can conbined usage.
Pharmaceutically acceptable carrier of the present invention can be that any preparation Chinese medicine oral liquid is commonly used or acceptable carrier in conventional pharmaceutical, includes but not limited to solvent (water, ethanol or propylene glycol), antiseptic (sodium benzoate, three pears acid and Nipagin ester) and surfactant (lecithin, tween) etc.
Preferably, Chinese medicine composition of the present invention is powder, oral liquid, granule, injection.
More preferably, described Chinese medicine composition is oral liquid, and wherein, in described Chinese medicine composition, Astragaloside content is 0.4-0.9mg/ml, and total sugar content is 100-180mg/ml.
Another object of the present invention is to the preparation method providing a kind of described Chinese medicine composition, comprising: part takes Radix Astragali 2-10 part, Radix Et Caulis Acanthopanacis Senticosi 1 part in proportion, extracting in water also filters, merging filtrate, concentrated.Specifically obtain by following methods:
Take the Radix Astragali, Radix Et Caulis Acanthopanacis Senticosi, coarse pulverization, soak, extract, filter, collect filtrate, filtering residue extracts 2 times again, and gradation is filtered, and merges 3 filtrates, centrifugal, supernatant concentration to relative density is the solution of 1.02-1.11, cold preservation, centrifugal, supernatant concentration is the clear paste of 1.15-1.25 to relative density, obtains Chinese medicine extraction concentrated solution, add pharmaceutically acceptable carrier, make the dosage form of the convenient application of veterinary clinic.
When preparing corresponding dosage form according to clinical needs, following post-processing step can be taked further:
(1) above-mentioned Chinese medicine extraction concentrated solution got by powder, spraying, dry, obtains described Chinese medicine powder.
(2) above-mentioned Chinese medicine extraction concentrated solution got by oral liquid, and be diluted with water to every milliliter containing primary crude drug 1.0-1.5g, filter, fill, every bottle of 100-200ml, 115 DEG C of high pressure 30min carry out sterilizing, obtain described Chinese medicine composition oral liquid.
(3) granule gets above-mentioned Chinese medicine extraction concentrated solution, is concentrated into the thick paste that relative density is 1.25-1.35, adds carrier, make granule, namely obtain described Chinese medicinal composition granules after drying.Preferably, described carrier is sucrose and dextrin, and the part by weight of thick paste, sucrose, dextrin is 1:2:1.
(4) injection gets above-mentioned Chinese medicine extraction concentrated solution, and be diluted with water to every milliliter containing primary crude drug 1-1.2g, add injection charcoal, filter, filtrate fine straining, injecting water use regulation pH value is 5.0-7.0, and sterilizing obtains described Chinese medicine composition composition injection.Preferably, described injection charcoal additional proportion is 0.5-1%.
Another object of the present invention is to provide described Chinese medicine composition to alleviate the application in the medicine of systemic immune response in preparation, and wherein, described application can use described Chinese medicine composition before and after immunity.
Preferably, in described application, subject is poultry, and birds comprises chicken, duck, goose; Domestic animal comprise pig, sheep.More preferably, described poultry are chicken, pig.
Preferably, in described application the usage of Chinese medicine composition and consumption as follows:
Usage:
Described Chinese medicine composition is peroral dosage form (powder, granule, oral liquid), in live vaccine immunity medication in first 3 days, is used in conjunction 3 days; In inactivated vaccine immunity medication simultaneously, be used in conjunction 3 days;
Described Chinese medicine composition is injection type (injection), in live vaccine immunity medication in first 3 days, and an administered intramuscular; In inactivated vaccine immunity medication simultaneously, an administered intramuscular.
Consumption:
When described Chinese medicine composition is powder: every premium on currency 0.2-0.4g; When described Chinese medicine composition is granule: every premium on currency 1.0-1.5g; When described Chinese medicine composition is oral liquid: every premium on currency 1ml; When described Chinese medicine composition is injection: every kg body weight injection 0.1-0.2ml.
Chinese medicine composition of the present invention by using before and after fowl poultry immune, vaccine immunity stress can be reduced, strengthen immune effect, the respiratory symptom such as snore, cough occurred after alleviating bird vaccine inoculation, improve that the appetite of livestock and poultry that systemic immune response causes declines, weightening finish slowly and fertility performance decline.
Detailed description of the invention
Specific embodiment is adopted to further describe the present invention below.
The preparation example of embodiment 1 Chinese medicine composition of the present invention
Preparation example 1: take the Radix Astragali 2 parts (2kg), Radix Et Caulis Acanthopanacis Senticosi 1 part (1kg), is ground into most coarse powder, adds 30L water, soak 1.5h, reflux, extract, 2h, filters, and collects filtrate, filtering residue extracting in water 2 times again, add water 25L at every turn, filters, merge 3 filtrates, centrifugal while hot, supernatant is evaporated to the solution that relative density is 1.05, and cold preservation 24h is centrifugal, supernatant is evaporated to the clear paste that relative density is 1.22, spraying dry, obtains Chinese prescription powder 1020g, is distributed into 50g/ bag, sealing, obtains Chinese medicine powder of the present invention.
Preparation example 2: take the Radix Astragali 3 parts (3kg), Radix Et Caulis Acanthopanacis Senticosi 1 part (1kg), is ground into most coarse powder, adds 40L water, soak 1.5h, reflux, extract, 2h, filters, and collects filtrate, filtering residue extracting in water 2 times again, add water 30L at every turn, filters, merge 3 filtrates, centrifugal while hot, supernatant is evaporated to the solution that relative density is 1.05, and cold preservation 24h is centrifugal, supernatant is evaporated to the clear paste that relative density is 1.22, spraying dry, obtains Chinese prescription powder 1308g, is distributed into 50g/ bag, sealing, obtains Chinese medicine powder of the present invention.
Preparation example 3: take the Radix Astragali 4 parts (4kg), Radix Et Caulis Acanthopanacis Senticosi 1 part (1kg), is ground into most coarse powder, adds 50L water, soak 1.5h, reflux, extract, 2h, filters, and collects filtrate, filtering residue extracting in water 2 times again, add water 48L at every turn, filters, merge 3 filtrates, centrifugal while hot, supernatant is evaporated to the solution that relative density is 1.11, and cold preservation 24h is centrifugal, supernatant is evaporated to the clear paste that relative density is 1.22, spraying dry, obtains Chinese prescription powder 1238g, is distributed into 50g/ bag, sealing, obtains Chinese medicine powder of the present invention.
Preparation example 4: take the Radix Astragali 6 parts (6kg), Radix Et Caulis Acanthopanacis Senticosi 1 part (1kg), is ground into most coarse powder, adds 70L water, soak 1.5h, reflux, extract, 2h, filters, and collects filtrate, filtering residue extracting in water 2 times again, add water 50L at every turn, filters, merge 3 filtrates, centrifugal while hot, supernatant is evaporated to the solution that relative density is 1.06, and cold preservation 24h is centrifugal, supernatant is evaporated to the clear paste that relative density is 1.15, spraying dry, obtains Chinese prescription powder 2339g, is distributed into 50g/ bag, sealing, obtains Chinese medicine powder of the present invention.
Preparation example 5: take the Radix Astragali 8 parts (8kg), Radix Et Caulis Acanthopanacis Senticosi 1 part (1kg), is ground into most coarse powder, adds 90L water, soak 1.5h, reflux, extract, 2h, filters, and collects filtrate, filtering residue extracting in water 2 times again, add water 85L at every turn, filters, merge 3 filtrates, centrifugal while hot, supernatant is evaporated to the solution that relative density is 1.09, and cold preservation 24h is centrifugal, supernatant is evaporated to the clear paste that relative density is 1.17, spraying dry, obtains Chinese prescription powder 3007g, is distributed into 50g/ bag, sealing, obtains Chinese medicine powder of the present invention.
Preparation example 6: take the Radix Astragali 10 parts (5kg), Radix Et Caulis Acanthopanacis Senticosi 1 part (0.5kg), is ground into most coarse powder, adds 50L water, soak 1.5h, reflux, extract, 2h, filters, and collects filtrate, filtering residue extracting in water 2 times again, add water 40L at every turn, filters, merge 3 filtrates, centrifugal while hot, supernatant is evaporated to the solution that relative density is 1.11, and cold preservation 24h is centrifugal, supernatant is evaporated to the clear paste that relative density is 1.18, spraying dry, obtains Chinese prescription powder 1873g, is distributed into 50g/ bag, sealing, obtains Chinese medicine powder of the present invention.
Preparation example 7: take the Radix Astragali 3 parts (3kg), Radix Et Caulis Acanthopanacis Senticosi 1 part (1kg), be ground into most coarse powder, add 40L water, soak 1.5h, reflux, extract, 2h, filter, collect filtrate, filtering residue extracting in water 2 times again, add water 35L at every turn, filter, merge 3 filtrates, centrifugal while hot, supernatant is evaporated to the solution that relative density is 1.02, cold preservation 24h, centrifugal, supernatant is evaporated to the clear paste that relative density is 1.24, continue to be concentrated into the thick paste 1.88kg that relative density is 1.29, add cream respectively and weigh the sucrose of 2 times and the dextrin of the heavy equivalent of cream, make granule, namely the granule 7.5kg of Chinese prescription of the present invention is obtained after carrying out drying, be distributed into 50g/ bag, sealing, obtain Chinese medicinal composition granules of the present invention.
Preparation example 8: take the Radix Astragali 4 parts (4kg), Radix Et Caulis Acanthopanacis Senticosi 1 part (1kg), be ground into most coarse powder, add 50L water, soak 1.5h, reflux, extract, 2h, filter, collect filtrate, filtering residue extracting in water 2 times again, add water 48L at every turn, filter, merge 3 filtrates, centrifugal while hot, supernatant is evaporated to the solution that relative density is 1.11, cold preservation 24h, centrifugal, supernatant is evaporated to the clear paste that relative density is 1.20, continue to be concentrated into the thick paste 2.36kg that relative density is 1.27, add cream respectively and weigh the sucrose of 2 times and the dextrin of the heavy equivalent of cream, make granule, namely the granule 9.4kg of Chinese prescription of the present invention is obtained after carrying out drying, be distributed into 50g/ bag, sealing, obtain Chinese medicinal composition granules of the present invention.
Preparation example 9: take the Radix Astragali 4 parts (4kg), Radix Et Caulis Acanthopanacis Senticosi 1 part (1kg), be ground into most coarse powder, add 50L water, soak 1.5h, reflux, extract, 2h, filter, collect filtrate, filtering residue extracting in water 2 times again, add water 45L at every turn, filter, merge 3 filtrates, centrifugal while hot, supernatant is evaporated to the solution that relative density is 1.08, cold preservation 24h, centrifugal, supernatant is evaporated to the clear paste that relative density is 1.19, be diluted with water to 5L (every milliliter containing primary crude drug 1g), filter, fill, every bottle of 200ml, 115 DEG C of high pressure 30min carry out sterilizing, obtain Chinese medicine oral liquid of the present invention.
Preparation example 10, take the Radix Astragali 6 parts (6kg), Radix Et Caulis Acanthopanacis Senticosi 1 part (1kg), be ground into most coarse powder, add 70L water, soak 1.5h, reflux, extract, 2h, filter, collect filtrate, filtering residue extracting in water 2 times again, add water 60L at every turn, filter, merge 3 filtrates, centrifugal while hot, supernatant is evaporated to the solution that relative density is 1.06, cold preservation 24h, centrifugal, supernatant is evaporated to the clear paste that relative density is 1.17, be diluted with water to 7L (every milliliter containing primary crude drug 1g), filter, fill, every bottle of 200ml, 115 DEG C of high pressure 30min carry out sterilizing, obtain the oral liquid of Chinese prescription of the present invention.
Preparation example 11: take the Radix Astragali 4 parts (4kg), Radix Et Caulis Acanthopanacis Senticosi 1 part (1kg), is ground into most coarse powder, adds 50L water, soaks 1.5h, reflux, extract, 2h, filters, and collects filtrate, filtering residue extracting in water 2 times again, add water 40L at every turn, filters, merge 3 filtrates, centrifugal while hot, supernatant is evaporated to the solution that relative density is 1.11, cold preservation 24h, centrifugal, supernatant be evaporated to relative density be 1.20 clear paste be about 3L, add 60g injection charcoal, stir 30-60min, filter, removing impurity.Filtrate, with 0.45 μm, aperture filter membrane fine straining, injects water to 8L, and regulate pH value to be 5.73, fill, every bottle of 50ml, 115 DEG C of high pressure 30min sterilizing, obtains Chinese medicine composition composition injection of the present invention.
Preparation example 12: take the Radix Astragali 10 parts (5kg), Radix Et Caulis Acanthopanacis Senticosi 1 part (0.5kg), is ground into most coarse powder, adds 50L water, soaks 1.5h, reflux, extract, 2h, filters, and collects filtrate, filtering residue extracting in water 2 times again, add water 40L at every turn, filters, merge 3 filtrates, centrifugal while hot, supernatant is evaporated to the solution that relative density is 1.11, cold preservation 24h, centrifugal, supernatant be evaporated to relative density be 1.20 clear paste be about 4L, add 50g injection charcoal, stir 30-60min, filter, removing impurity.Filtrate, with 0.45 μm, aperture filter membrane fine straining, injects water to 5.5L, and regulate pH value to be 6.22, fill, every bottle of 50ml, 115 DEG C of high pressure 30min sterilizing, obtains Chinese medicine composition composition injection of the present invention.
Embodiment 2 Chinese medicine composition of the present invention inoculates the effect of the systemic immune response caused to veterinary vaccines
1. Chinese medicine composition of the present invention is tested the impact of the systemic immune response that chicken newcastle, infectiousness bronchitis bigeminy live vaccination cause
Medicine and vaccine: the Chinese medicine powder in embodiment 1 prepared by preparation example 1,2,3,4,5,6.Newcastle disease, infectiousness bronchitis bigeminy live vaccine (La sota strain+H120 strain), 1000 plumage part/bottles, lot number: 121009, containing newcastle disease virus La Sota strain >=106.0EID50/ plumage part; Containing avian infectious bronchitis virus H120 strain >=103.5EID50/ plumage part, Qilu Animal Health Products Co., Ltd. produces.
Test method: select 1 age in days Avain chicken 240, is divided into A, B, C, D, E, F, G, H8 group at random, often organizes 30.Each group when carrying out feeding and management to 4 age in days by the normal feeding standard of broiler, takes each group of chicken body weight, then to the chicken administration respectively of A, B, C, D, E, F these 6 group.During to 7 age in days, after taking each group of chicken body weight, then for A, B, C, D, E, F, G these 7 group chicken by only use newcastle disease, infectiousness bronchitis bigeminy live vaccine 2 plumage part collunarium, eye dripping immunity.Wherein G group immunity not administration is as immunized controls group, and the not immune also not administration of H group is as blank group.Each group specifically divide into groups and processing method in table 1.
Table 1 tests concrete grouping and processing method
Observe the mental status, the behavior expression of chicken group after immunity every day, record each group of feed intake, Continuous Observation 7 days.Immunity latter 7 days (14 age in days), takes test Ge Zu chicken colony heavy, the rate of body weight gain of each group of Computation immunity compared with blank group.
Result of the test: after immunity, each group spirit still can.But after immunity the 3rd day, do not rob the symptoms such as food after G group and immunized controls group 5 chickens occur getting rid of head, feed, can hear Grunting in quiet situation when the 5th day, other each group without this phenomenon.Each group of chicken group weighs, feed and fertility performance statistical conditions refer to table 2.
Each group of fertility performance statistical conditions tested by table 2
As can be seen from the test results, the Chinese medicine powder of preparation example of the present invention is used afterwards in immunity, the respiratory symptom that vaccine immunity causes can be improved, search for food in 1 week after each medication group Immune Profile In Chicks normal, Gain weight, feed-weight ratio and blank group are substantially suitable, some group even exceeds blank group as C, D, F group etc., illustrates that use this prescription Chinese medicine composition to resist chicken that vaccine immunity causes is mined massively food decline, poor growth and feed-weight ratio rising in a short time, price of deed reduction.
2. Chinese medicine composition of the present invention inoculates the impact test of the systemic immune response caused on laying hen newcastle disease, infectious bronchitis, bird flu triple inactivated vaccine
Medicine and vaccine: the Chinese medicine powder in embodiment 1 prepared by preparation example 2,3,4,5.Newcastle disease, infectious bronchitis, bird flu triple inactivated vaccine (La sota strain+M41 strain+H9 hypotype HL strain), lot number 120523, Qilu Animal Health Products Co., Ltd. produces.
Test method: Zhaoyang Town one, Mengjin County, Luoyang City plant, selects the 250 blue brown laying hens 6000 in age in days sea, is divided into A, B, C, D, E5 group at random, often organizes each 1200.The average egg production of immunity first 1 week these chickens is 92.87%.When 250 age in days, use newcastle disease, infectious bronchitis, bird flu triple inactivated vaccine 0.3ml to carry out immunity inoculation to each group of chicken intramuscular injection, wherein the chicken immunity of A, B, C, D4 group is simultaneously respectively to the Chinese medicine powder taking preparation example 2 in the embodiment of the present invention 1, preparation example 3, preparation example 4, preparation example 5 preparation, mixed drink, be used in conjunction 3 days, the not medication of E group is as blank group.The concrete grouping of test and disposition are in table 3.
Table 3 tests concrete grouping and disposition
Add up the egg number of each group of chicken after immunity every day, calculate laying rate, the change of each group chicken group laying rate after observing immunity, Continuous Observation 14 days.
Laying rate=this group receives egg piece number/this group chicken sum × 100%
Result of the test: after immunity, the laying rate statistical table of each group chicken group every day is in table 4.
Each group of chicken group laying rate statistical table tested by table 4
Note: " * " represents compared with blank group, significant difference (0.01 < P < 0.05), " * * " represents compared with blank group, and difference extremely significantly (P < 0.01).
As can be seen from Table 4, use the Chinese medicine composition of preparation example of the present invention in immunity afterwards, can improve the decline of laying rate in a short time that vaccination causes, administration respectively organizes the laying rate of chicken group compared with blank group, significant difference.Administration respectively organizes the laying rate after immunity for chickens compared with (92.87%) before immunity, substantially without significantly declining, degradation systemic immune response under the laying rate that this Chinese medicine composition can resist caused by vaccination is described.
3. Chinese medicine composition of the present invention is on the impact test of the systemic immune response that infectious serositis in duck, the inoculation of colibacillosis bigeminy inactivated propolis vaccines produce
Medicine and vaccine: the Chinese medicine powder in embodiment 1 prepared by preparation example 2,3,4,5.Infectious serositis in duck, colibacillosis bigeminy inactivated propolis vaccines (WF strain+BZ strain), 250ml/ bottle, 110215, Shandong Hua Hong biological engineering company limited produces.
Test method: select 1 age in days cherry valley duck 15000, from 5 duck canopies of same Animal Raising Zone, is divided into A, B, C, D, E, F6 group at random in units of duck canopy.Each group is carried out feeding and management to 7 age in days by normal feeding standard, often group randomly draws 50 ducks as subjects, take Ge Zu duck colony heavy, to A, B, C, D, E5 the duck organized only by only carrying out immunity with infectious serositis in duck, colibacillosis bigeminy inactivated propolis vaccines cervical region subcutaneous injection, every duck injection 0.3ml, simultaneously to the duck only administration respectively of A, B, C, D these 4 group, the not administration of E group is as immunized controls group.The not immune also not administration of F group is as blank group.The concrete grouping of test and processing method are in table 6.
Table 6 tests concrete grouping and processing method
The feed intake of each group of duck group is recorded every day, Continuous Observation 7 days after immunity.Immunity latter 7 days (14 age in days), takes Ge Zu duck colony heavy, the rate of body weight gain of each group of Computation immunity compared with blank group.
Result of the test: each group duck group weighs, feed and fertility performance statistical conditions refer to table 7.
Each group of fertility performance statistical conditions tested by table 7
As can be seen from the test results, search for food in 1 week after immunity uses the Chinese medicine powder medication of preparation example of the present invention respectively to organize duck group immunity afterwards normal, Gain weight, feed-weight ratio and blank group are substantially suitable, illustrates mine massively use this Chinese medicine composition to resist duck that vaccination causes food decline, poor growth and price of deed reduction in a short time.
4. Chinese medicine composition of the present invention inoculates the impact test of the systemic immune response caused on piglet porcine circovirus inactivated vaccine
Medicine and vaccine: the Chinese medicine composition composition injection of preparation example 11,12 gained in the embodiment of the present invention 1.Porcine circovirus 2 type inactivated vaccine (SH strain), lot number: 120802, every part viral level >=107.0TCID50, Wuhan Keqian Animal Biological Products Co., Ltd..
Test method: pig farm, Yichuan County of Luoyang City, selects 28 age in days DLY ternary piglet 80, is divided into A, B, C, D4 group at random, often organizes 20, and subfield is raised, by only weighing and recording pig numbering.When 30 age in days, porcine circovirus 2 type inactivated vaccine (SH strain) immunity inoculation is used to the pig of A, B, C3 group, intramuscular injection, every pig 1ml.A group gives the Chinese medicine composition composition injection of preparation example 11 gained in the embodiment of the present invention 1 while immunity, and dosage is 0.1ml/kg body weight.B group gives the Chinese medicine composition composition injection of preparation example 12 gained in the embodiment of the present invention 1 while immunity, and dosage is 0.1ml/kg body weight.C group normal immunological, not administration, as immunized controls group.D group is not immune also not administration after weighing, as blank group.Immunity each group of 14d(i.e. 44 ages in days after immunity) repeat immunity 1 time.When 65 ages in days turn group, to the pig of each group of test by only weighing, record body weight growth pattern, the rate of body weight gain of each group of Computation immunity compared with blank group, and compare with SPSS11.5 statistical analysis software.Result of the test: test the growing state of each group of pig in table 5.
The growing state of each group of pig tested by table 5
Note: with in a line, indicates different alphabetical person and represents that difference extremely significantly (P < 0.01).
As can be seen from result of the test, the pig of the immunized controls group (C group) of Chinese medicine composition composition injection is not given after vaccinate, compared with blank group (D group), its body weight within the child care phase increases significantly on the low side, and difference extremely significantly (P < 0.01); But give the immune group A of Chinese medicine composition composition injection of the present invention and the pig of B during vaccinate, although 2 immunity of whole child care phase, the growth of its body weight is not affected substantially simultaneously.Illustrate that this Chinese medicine composition can resist the pig growth promoter that vaccine injection causes and be obstructed.
5. conclusion
Chinese medicine composition of the present invention, use before and after fowl poultry immune, can improve that the appetite that poultry occur after vaccination declines, increase weight the phenomenon such as slow, degradation systemic immune response under the respiratory symptom such as snore, cough occurred after alleviating bird vaccine inoculation and laying rate.
The foregoing is only better embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (12)
1. a Chinese medicine composition, wherein, described traditional Chinese medicine composite is Radix Astragali 2-10 part, Radix Et Caulis Acanthopanacis Senticosi 1 part.
2. Chinese medicine composition according to claim 1, wherein, described traditional Chinese medicine composite is Radix Astragali 3-6 part, Radix Et Caulis Acanthopanacis Senticosi 1 part.
3. Chinese medicine composition according to claim 1, wherein, described Chinese medicine composition comprises carrier further, and described carrier comprises diluent, disintegrating agent, inclusion agents, wetting agent.
4. Chinese medicine composition according to claim 1, wherein, described Chinese medicine composition is powder, oral liquid, granule, injection.
5. Chinese medicine composition according to claim 4, described Chinese medicine composition is oral liquid, and wherein, in described Chinese medicine composition, Astragaloside content is 0.4-0.9mg/ml, and total sugar content is 100-180mg/ml.
6. the preparation method of Chinese medicine composition as described in any one of Claims 1 to 5, described method comprises:
Take the Radix Astragali, Radix Et Caulis Acanthopanacis Senticosi decoction pieces, coarse pulverization, soaks, extract, filter, collect filtrate, filtering residue extracts 2 times again, and gradation is filtered, and merges 3 described filtrates, centrifugal, supernatant concentration to relative density is the solution of 1.02-1.11, cold preservation, centrifugal, supernatant concentration is the clear paste of 1.15-1.25 to relative density, obtains Chinese medicine extraction concentrated solution, add pharmaceutically acceptable carrier, make the dosage form of the convenient application of veterinary clinic.
7. preparation method according to claim 6, described method comprises post-processing step further:
Get described Chinese medicine extraction concentrated solution, spraying, dry, obtain described Chinese medicine powder; Or
Get described Chinese medicine extraction concentrated solution, be diluted with water to every milliliter containing primary crude drug 1.0-1.5g, filter, sterilizing, obtains described Chinese medicine composition oral liquid; Or
Get described Chinese medicine extraction concentrated solution, be concentrated into the thick paste that relative density is 1.25-1.35, add carrier, make granule, after drying, namely obtain described Chinese medicinal composition granules; Or
Get described Chinese medicine extraction concentrated solution, be diluted with water to every milliliter containing primary crude drug 1-1.2g, add injection charcoal, filter, filtrate fine straining, inject with water, adjustment pH value is 5.0-7.0, sterilizing, obtains described Chinese medicine composition composition injection.
8. the Chinese medicine composition according to any one of Claims 1 to 5 alleviates the application in the medicine of systemic immune response in preparation, and wherein, described application can use described Chinese medicine composition before and after immunity.
9. application according to claim 8, wherein, subject is poultry, and birds comprises chicken, duck, goose; Domestic animal comprise pig, sheep.
10. application according to claim 9, wherein, described poultry are chicken, pig.
11. application according to claim 8, wherein,
Described Chinese medicine composition dosage form is peroral dosage form, in live vaccine immunity medication in first 3 days, is used in conjunction 3 days; In inactivated vaccine immunity medication simultaneously, be used in conjunction 3 days; Or
Described Chinese medicine composition dosage form is injection type, in live vaccine immunity medication in first 3 days, and an administered intramuscular; In inactivated vaccine immunity medication simultaneously, an administered intramuscular.
12. application according to claim 8, wherein, when described Chinese medicine composition dosage form is powder: every premium on currency 0.2-0.4g; When described Chinese medicine composition dosage form is granule: every premium on currency 1.0-1.5g; When described Chinese medicine composition dosage form is oral liquid: every premium on currency 1ml; When described Chinese medicine composition dosage form is injection: every kg body weight injection 0.1-0.2ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310639196.4A CN104666399A (en) | 2013-12-02 | 2013-12-02 | Traditional Chinese medicine composition and preparation method and applications thereof |
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| CN106214803A (en) * | 2016-08-23 | 2016-12-14 | 牛金鹏 | A kind of herbal medicine preventing and treating chicken vaccine immunity stress |
| CN106466478A (en) * | 2015-08-17 | 2017-03-01 | 洛阳惠中兽药有限公司 | A kind of immune composition, contain said composition vaccine and its application |
| CN106890212A (en) * | 2015-12-18 | 2017-06-27 | 洛阳惠中兽药有限公司 | Application of the slender acanthopanax stilbene Chinese medicine composition in prevention and treatment porcine respiratory disease syndrome |
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| CN111494499A (en) * | 2020-04-26 | 2020-08-07 | 江西中成中药原料有限公司 | Astragalus membranaceus and acanthopanax senticosus soluble powder for poultry |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106466478A (en) * | 2015-08-17 | 2017-03-01 | 洛阳惠中兽药有限公司 | A kind of immune composition, contain said composition vaccine and its application |
| CN105193950A (en) * | 2015-10-22 | 2015-12-30 | 成都乾坤动物药业有限公司 | Traditional Chinese veterinary medicine for improving animal immunity and preparation method as well as application thereof |
| CN106890212A (en) * | 2015-12-18 | 2017-06-27 | 洛阳惠中兽药有限公司 | Application of the slender acanthopanax stilbene Chinese medicine composition in prevention and treatment porcine respiratory disease syndrome |
| CN106214803A (en) * | 2016-08-23 | 2016-12-14 | 牛金鹏 | A kind of herbal medicine preventing and treating chicken vaccine immunity stress |
| CN109125382A (en) * | 2018-10-30 | 2019-01-04 | 河南后羿实业集团有限公司 | A kind of compound Chinese medicinal preparation and preparation method thereof enhancing chicken immune regulatory function |
| CN111494499A (en) * | 2020-04-26 | 2020-08-07 | 江西中成中药原料有限公司 | Astragalus membranaceus and acanthopanax senticosus soluble powder for poultry |
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Application publication date: 20150603 |