CN101703772A - Compound indigowoad root oral liquid for improving poultry immunity and preparation method thereof - Google Patents
Compound indigowoad root oral liquid for improving poultry immunity and preparation method thereof Download PDFInfo
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- CN101703772A CN101703772A CN200910308228A CN200910308228A CN101703772A CN 101703772 A CN101703772 A CN 101703772A CN 200910308228 A CN200910308228 A CN 200910308228A CN 200910308228 A CN200910308228 A CN 200910308228A CN 101703772 A CN101703772 A CN 101703772A
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Abstract
The invention discloses compound indigowoad root oral liquid for improving poultry immunity and a preparation method thereof and aims at providing compound indigowoad root oral liquid for improving organism immunity and increases vaccine immunity effect and a preparation method thereof. Each 100 liters of the oral liquid comprises the following components: 0.5 to 20kg of indigowoad root amylose, 0.5 to 20kg of astragalus amylose raw material medicament containing 50 percent of astragalus amylose, 0.5 to 10kg of epimedium amylose raw material medicament containing 50 percent of epimedium amylose, 0.5 to 10kg of morinda extract, 50g of potassium sorbate and the balance of injection water. In the method, an amylose component with immunologic enhancement effect is selected from an indigowoad root medicinal material and mixed with restorative Chinese medicaments with the same immunologic enhancement effect, such as astragalus, epimedium and morinda, to produce a synergetic effect to enhance treatment effect. The oral liquid has good treatment effect on low immunity of poultry caused by disease or breeding conditions and can strengthen immune effect of a vaccine.
Description
Technical field
The present invention relates to a kind of compound indigowoad root oral liquid of improving poultry immunity and preparation method thereof that is used to.
Background technology
In the prevention and control of fowl infectious disease, though the mesogenic live vaccine can be broken through the interference of maternal antibody to a certain extent, reduce the clinical death that virulent strain and highly virulent strain are caused, but because itself has to a certain degree damage to the poultry immunity organ, thereby influence body's immunological function, add the especially interaction of inhibitive ability of immunity virulence factor and stressors etc. of various virulence factors in the environment, make that the prevention and control of viral infectious are difficult more.Aquaculture is badly in need of the immunostimulant and the new vaccine adjuvant of high-efficiency low-toxicity at present, to improve the immune effect of vaccine.
In the aquaculture, Radix Isatidis is mainly used in the control of virosis at present.Radix Isatidis belongs to heat and toxic materials clearing away medicine in the traditional Chinese medical science, and tool has certain effect in the prevention and control of avian viral infectious disease.But in the various preparation production processes of Radix Isatidis, the Radix Isatidis polysaccharide goes out of use as impurity owing to do not possess direct antivirus action always at present.
Summary of the invention
The present invention be directed to the weak point in the present bird vaccine immune effect, the compound indigowoad root oral liquid of a kind of human body immunity improving power, increase immune effect of vaccine is provided.
Another object of the present invention provides a kind of technology above-mentioned preparation method that is used to improve the compound indigowoad root oral liquid of immunity of fowl simple, that realize easily.
The present invention is achieved through the following technical solutions:
A kind of compound indigowoad root oral liquid that is used to improve poultry immunity, it is characterized in that, every 100L oral liquid is made by following component: Radix Isatidis polysaccharide 0.5～10kg, astragalus polysaccharides content is astragalus polysaccharides crude drug 0.5～20kg of 50%, epimedium brevicornum polysaccharide content is epimedium brevicornum polysaccharide crude drug 0.5～10kg of 50%, Radix Morindae Officinalis extract 0.5～5kg, potassium sorbate 50g, the water for injection of surplus.
A kind of preparation method that is used to improve the compound indigowoad root oral liquid of poultry immunity, it is characterized in that, comprise the steps: to take by weighing Radix Isatidis polysaccharide 0.5～10kg, astragalus polysaccharides content is astragalus polysaccharides crude drug 0.5～20kg of 50%, epimedium brevicornum polysaccharide content is epimedium brevicornum polysaccharide crude drug 0.5～10kg of 50%, Radix Morindae Officinalis extract 0.5～5kg puts into water for injection 30L, is stirred to whole dissolvings; Add potassium sorbate 50g, add water for injection again, stir, keep 1520min, treat that medicinal liquid promptly makes the compound indigowoad root oral liquid that is used to improve poultry immunity after clear and bright to 100L.
Wherein, the preferable of every 100L oral liquid consists of: Radix Isatidis polysaccharide 0.8～5kg, astragalus polysaccharides content is astragalus polysaccharides crude drug 0.8～5kg of 50%, epimedium brevicornum polysaccharide content is epimedium brevicornum polysaccharide crude drug 0.8～3kg of 50%, Radix Morindae Officinalis extract 0.8～3kg, potassium sorbate 50g, the water for injection of surplus.
The best group of every 100L oral liquid becomes: Radix Isatidis polysaccharide 3.5kg, astragalus polysaccharides content is 50% astragalus polysaccharides crude drug 3kg, and epimedium brevicornum polysaccharide content is 50% epimedium brevicornum polysaccharide crude drug 2kg, Radix Morindae Officinalis extract 1.5kg, potassium sorbate 50g, the water for injection of surplus.
Wherein, every g Radix Isatidis polysaccharide is equivalent to 10g Radix Isatidis raw medicinal herbs, and every g Radix Morindae Officinalis extract is equivalent to 10g Radix Morindae Officinalis raw medicinal herbs.
The present invention has following technique effect:
The compound oral liquid of the present invention for adopting Radix Isatidis polysaccharide and astragalus polysaccharides, epimedium brevicornum polysaccharide, Radix Morindae Officinalis extract to make.What have immunological enhancement in the Radix Isatidis mainly is the polysaccharide component that precipitate with ethanol obtains in the leaching process, and specific immunity and nonspecific immunity, humoral immunization and cellular immunization are all had certain facilitation.Astragalus polysaccharides has immunoregulatory activity, significantly enhancing non-specific immunity function and humoral immune function, significantly the phagocytic function of enhancing macrophage promotes serum hemolysin to form, and raising plaque forming cells's haemolysis function and tangible carbon are from effect of cleaning up and the obvious spleen weight that increases.Epimedium brevicornum polysaccharide has the effect of enhancing human body immunity function, by the influence to the spleen antibody-producting cell, has both increased spleen antibody-producting cell number, promotes each plasma cell to produce antibody again.Contain compositions such as flavonoid, anthraquinone, saccharide in the Radix Morindae Officinalis extract, can suppress atrophy of thymus gland, leukocyte in the rising blood, thus play the effect of enhancing immunity.The present invention selects to have in the Baphicacanthus cusia root herb polysaccharide component of immunoenhancement result, with the tonification class Chinese medicine astragalus, Herba Epimedii, the Radix Morindae Officinalis compatibility that have immunological enhancement equally, play synergism, heighten the effect of a treatment, because the hypoimmunity that disease or raising condition cause has good efficacy, can strengthen immune effect of vaccine simultaneously for birds.
The preparation method technology of the compound indigowoad root oral liquid that is used to improve poultry immunity of the present invention is simple, realizes easily.
The specific embodiment
The present invention is described in detail below in conjunction with specific embodiment.
The commercially available prod of Radix Isatidis polysaccharide for adopting conventional precipitate with ethanol extraction process to extract, also can adopt following prepared: the Radix Isatidis raw medicinal herbs adds 2 times of water gagings and decocts 2h, filters and obtains filtrate.Decoct 3 times, merge the filtrate of three decoctions, filter, filtrate decompression is concentrated into 1: 1, adds 2 times of amount 95% ethanol precipitate with ethanol 24h, washs successively with dehydrated alcohol, acetone, absolute ether, and vacuum drying gets the Radix Isatidis polysaccharide.Every g Radix Isatidis polysaccharide is equivalent to 10g Radix Isatidis raw medicinal herbs approximately.
Radix Morindae Officinalis extract is for adopting the commercially available prod that disclosed extracting method extracts in " medicinal herb components extraction separation and preparation processing new technique new technology new standard " (Chinese education publishing house), and every g Radix Morindae Officinalis extract is equivalent to 10g Radix Morindae Officinalis raw medicinal herbs.
Astragalus polysaccharides content is that 50% astragalus polysaccharides crude drug, epimedium brevicornum polysaccharide content are that 50% epimedium brevicornum polysaccharide crude drug is the commercially available prod.
Embodiment 1
Take by weighing Radix Isatidis polysaccharide 0.5kg, astragalus polysaccharides content is 50% astragalus polysaccharides crude drug 0.5kg, and epimedium brevicornum polysaccharide content is 50% epimedium brevicornum polysaccharide crude drug 10kg, and Radix Morindae Officinalis extract 5kg puts into water for injection 30L, is stirred to whole dissolvings.Add potassium sorbate 50g, add water for injection again, stir, keep 15-20min, treat that medicinal liquid promptly makes the compound indigowoad root oral liquid that is used to improve poultry immunity after clear and bright to 100L.
Embodiment 2
Radix Isatidis polysaccharide 10kg, astragalus polysaccharides content are 50% astragalus polysaccharides crude drug 20kg, and epimedium brevicornum polysaccharide content is 50% epimedium brevicornum polysaccharide crude drug 0.5kg, and Radix Morindae Officinalis extract 0.5kg puts into water for injection 30L, is stirred to whole dissolvings; Add potassium sorbate 50g, add water for injection again, stir, keep 15-20min, treat that medicinal liquid promptly makes the compound indigowoad root oral liquid that is used to improve poultry immunity after clear and bright to 100L.
Embodiment 3
Radix Isatidis polysaccharide 0.8kg, astragalus polysaccharides content are 50% astragalus polysaccharides crude drug 0.8kg, and epimedium brevicornum polysaccharide content is 50% epimedium brevicornum polysaccharide crude drug 3kg, and Radix Morindae Officinalis extract 3kg puts into water for injection 30L, is stirred to whole dissolvings; Add potassium sorbate 50g, add water for injection again, stir, keep 15-20min, treat that medicinal liquid promptly makes the compound indigowoad root oral liquid that is used to improve poultry immunity after clear and bright to 100L.
Embodiment 4
Radix Isatidis polysaccharide 5kg, astragalus polysaccharides content are 50% astragalus polysaccharides crude drug 5kg, and epimedium brevicornum polysaccharide content is 50% epimedium brevicornum polysaccharide crude drug 0.8kg, and Radix Morindae Officinalis extract 0.8kg puts into water for injection 30L, is stirred to whole dissolvings; Add potassium sorbate 50g, add water for injection again, stir, keep 15-20min, treat that medicinal liquid promptly makes the compound indigowoad root oral liquid that is used to improve poultry immunity after clear and bright to 100L.
Embodiment 5
Radix Isatidis polysaccharide 3kg, astragalus polysaccharides content are 50% astragalus polysaccharides crude drug 2kg, and epimedium brevicornum polysaccharide content is 50% epimedium brevicornum polysaccharide crude drug 1.5kg, and Radix Morindae Officinalis extract 1kg puts into water for injection 30L, is stirred to whole dissolvings; Add potassium sorbate 50g, add water for injection again, stir, keep 15-20min, treat that medicinal liquid promptly makes the compound indigowoad root oral liquid that is used to improve poultry immunity after clear and bright to 100L.
Embodiment 6
Radix Isatidis polysaccharide 3.5kg, astragalus polysaccharides content are 50% astragalus polysaccharides crude drug 3kg, and epimedium brevicornum polysaccharide content is 50% epimedium brevicornum polysaccharide crude drug 2kg, and Radix Morindae Officinalis extract 1.5kg puts into water for injection 30L, is stirred to whole dissolvings; Add potassium sorbate 50g, add water for injection again, stir, keep 15-20min, treat that medicinal liquid promptly makes the compound indigowoad root oral liquid that is used to improve poultry immunity after clear and bright to 100L.
Compound indigowoad root oral liquid of the present invention can be used for birdss such as chicken, duck, goose.Be that experimental animal describes with young cock below.
Clinical trial:
1. laboratory animal:
100 of the healthy young cocks of 1 age in days.
2. experiment medicine:
The compound indigowoad root oral liquid our unit development that is used to improve poultry immunity of the embodiment of the invention 6 preparations;
Astragalus polysaccharides oral liquid our unit prepared in laboratory;
Levamisole our unit prepared in laboratory.
3. experimental technique:
100 young cocks are divided into four groups at random, 25 every group.
The A group does not give medicine for the blank group.
B group is the astragalus polysaccharides matched group, usage: astragalus polysaccharides oral liquid mixes with water that to obtain mass percent concentration be that 0.1% astragalus polyose solution is freely drunk.In 14 ages in days immunity beginning in preceding 2 days administration, logotype 4 days, 17 age in days venous blood collections, conventional method is calculated total lymphocyte count.In 21 ages in days immunity beginning in preceding 2 days administration, logotype 4 days, 24 age in days venous blood collections, conventional method is calculated total lymphocyte count.In 28 ages in days immunity beginning in preceding 2 days administration, logotype 4 days, 31 age in days venous blood collections, conventional method is calculated total lymphocyte count.
The C group is the levamisole matched group, usage: drinking-water, 10mg/kg body weight/day.In 14 ages in days immunity beginning in preceding 2 days administration, logotype 4 days, 17 age in days venous blood collections, conventional method is calculated total lymphocyte count.In 21 ages in days immunity beginning in preceding 2 days administration, logotype 4 days, 24 age in days venous blood collections, conventional method is calculated total lymphocyte count, in 28 ages in days immunity beginning in preceding 2 days administration, logotype 4 days, 31 age in days venous blood collections, conventional method is calculated total lymphocyte count.
The compound indigowoad root oral liquid that D group improves poultry immunity with the embodiment of the invention 6 being used to of preparing is fed usage: compound indigowoad root oral liquid mixes with water that to obtain mass percent concentration be that 1% solution is freely drunk.In 14 ages in days immunity beginning in preceding 2 days administration, logotype 4 days, 17 age in days venous blood collections, conventional method is calculated total lymphocyte count.In 21 ages in days immunity beginning in preceding 2 days administration, logotype 4 days, 24 age in days venous blood collections, conventional method is calculated total lymphocyte count, in 28 ages in days immunity beginning in preceding 2 days administration, logotype 4 days, 31 age in days venous blood collections, conventional method is calculated total lymphocyte count.
Result of the test is as shown in table 1.
4. conclusion:
Analyze by statistics from the result of the test of table 1, the compound indigowoad root oral liquid group that is used to improve poultry immunity of the embodiment of the invention 6,0.1% astragalus polysaccharides group and levamisole group have all improved lymphocyte quantity, with blank group difference extremely significantly (P＜0.01).Use the lymphocyte number of oral liquid of the present invention to be higher than other two kinds of medicine groups.Illustrate that the present invention has the effect that improves poultry immunity preferably, can strengthen immune effect of vaccine.
Table 1
Claims (6)
1. compound indigowoad root oral liquid that is used to improve poultry immunity, it is characterized in that, every 100L oral liquid is made by following component: Radix Isatidis polysaccharide 0.5～10kg, astragalus polysaccharides content is astragalus polysaccharides crude drug 0.5～20kg of 50%, epimedium brevicornum polysaccharide content is epimedium brevicornum polysaccharide crude drug 0.5～10kg of 50%, Radix Morindae Officinalis extract 0.5～5kg, potassium sorbate 50g, the water for injection of surplus.
2. the compound indigowoad root oral liquid that is used to improve poultry immunity according to claim 1, it is characterized in that, every 100L oral liquid is made by following component: Radix Isatidis polysaccharide 0.8～5kg, astragalus polysaccharides content is astragalus polysaccharides crude drug 0.8～5kg of 50%, epimedium brevicornum polysaccharide content is epimedium brevicornum polysaccharide crude drug 0.8～3kg of 50%, Radix Morindae Officinalis extract 0.8～3kg, potassium sorbate 50g, the water for injection of surplus.
3. the compound indigowoad root oral liquid that is used to improve poultry immunity according to claim 1, it is characterized in that, every 100L oral liquid is made by following component: Radix Isatidis polysaccharide 3.5kg, astragalus polysaccharides content is 50% astragalus polysaccharides crude drug 3kg, epimedium brevicornum polysaccharide content is 50% epimedium brevicornum polysaccharide crude drug 2kg, Radix Morindae Officinalis extract 1.5kg, potassium sorbate 50g, the water for injection of surplus.
4. according to each described compound indigowoad root oral liquid that is used to improve poultry immunity in the claim 1 to 3, it is characterized in that described every g Radix Isatidis polysaccharide is equivalent to 10g Radix Isatidis raw medicinal herbs, every g Radix Morindae Officinalis extract is equivalent to 10g Radix Morindae Officinalis raw medicinal herbs.
5. the described preparation method that is used to improve the compound indigowoad root oral liquid of poultry immunity of a claim 1, it is characterized in that, comprise the steps: to take by weighing Radix Isatidis polysaccharide 0.5～10kg, astragalus polysaccharides content is astragalus polysaccharides crude drug 0.5～20kg of 50%, epimedium brevicornum polysaccharide content is epimedium brevicornum polysaccharide crude drug 0.5～10kg of 50%, Radix Morindae Officinalis extract 0.5～5kg puts into water for injection 30L, is stirred to whole dissolvings; Add potassium sorbate 50g, add water for injection again, stir, keep 15-20min, treat that medicinal liquid promptly makes the compound indigowoad root oral liquid that is used to improve poultry immunity after clear and bright to 100L.
6. the compound indigowoad root oral liquid that is used to improve poultry immunity according to claim 5 is characterized in that, described every g Radix Isatidis polysaccharide is equivalent to 10g Radix Isatidis raw medicinal herbs, and every g Radix Morindae Officinalis extract is equivalent to 10g Radix Morindae Officinalis raw medicinal herbs.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910308228A CN101703772A (en) | 2009-10-13 | 2009-10-13 | Compound indigowoad root oral liquid for improving poultry immunity and preparation method thereof |
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| CN200910308228A CN101703772A (en) | 2009-10-13 | 2009-10-13 | Compound indigowoad root oral liquid for improving poultry immunity and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102451204A (en) * | 2010-12-17 | 2012-05-16 | 长春新安药业有限公司 | Quality control method of compound indigowoad root oral liquid |
| CN103222980A (en) * | 2013-05-14 | 2013-07-31 | 海南师范大学 | Environment-friendly chick immunopotentiator |
| US20130259809A1 (en) * | 2010-10-09 | 2013-10-03 | State Key Laboratory Of Respiratory Diseases | Use of polysaccharides from radix isatidis in manufacture of medicaments against influenza virus |
-
2009
- 2009-10-13 CN CN200910308228A patent/CN101703772A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130259809A1 (en) * | 2010-10-09 | 2013-10-03 | State Key Laboratory Of Respiratory Diseases | Use of polysaccharides from radix isatidis in manufacture of medicaments against influenza virus |
| CN102451204A (en) * | 2010-12-17 | 2012-05-16 | 长春新安药业有限公司 | Quality control method of compound indigowoad root oral liquid |
| CN103222980A (en) * | 2013-05-14 | 2013-07-31 | 海南师范大学 | Environment-friendly chick immunopotentiator |
| CN103222980B (en) * | 2013-05-14 | 2014-07-09 | 海南师范大学 | Environment-friendly chick immunopotentiator |
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Application publication date: 20100512 |