CN101019885A - Compound ginseng-astragalus immunopotentiator - Google Patents
Compound ginseng-astragalus immunopotentiator Download PDFInfo
- Publication number
- CN101019885A CN101019885A CN 200710019342 CN200710019342A CN101019885A CN 101019885 A CN101019885 A CN 101019885A CN 200710019342 CN200710019342 CN 200710019342 CN 200710019342 A CN200710019342 A CN 200710019342A CN 101019885 A CN101019885 A CN 101019885A
- Authority
- CN
- China
- Prior art keywords
- astragalus
- agent
- chinese medicine
- ginseng
- compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The compound ginseng-astragalus immunopotentiator for enhancing immunological function of animal is injection, each 1000 ml of which contains ginsenoside 0.75 g and astragalus polysaccharide 3.0 g except water for injection. The compound ginseng-astragalus immunopotentiator may be used alone to enhance the cellular immunity and humoral immunity or as immunological adjuvant to result in excellent immunopotentiating effect. Pharmacological test shows that the immunopotentiator can raise the expression of chicken's peripheral blood T lymphocyte IL-2mRNA and IFN-gamma-mRNA. Safety test shows that the immunopotentiator has no irritation and no toxicity.
Description
One, technical field
A kind of novel compound ginseng-astragalus Chinese medicine ingredients immunostimulant of the present invention abbreviates the ginseng stilbene as and increases and exempt from agent, belongs to the novel immunostimulant that strengthens immunologic function of livestock and birds or the immunologic adjuvant of raising zoonosis immune effect of vaccine.
Two, technical background
The poultry infectious disease is the great viral infectious of matter of utmost importance, the especially current popular of harm animal husbandry development always, does not still have effective Therapeutic Method so far, and vaccine prevention is still topmost measure.For various reasons, most vaccines have only and immunostimulant or the good epidemic prevention effect of immunological adjuvant fit applications competence exertion.But, at present chemical immunological adjuvants such as the newborn class of domestic and international widely used oil, aluminium glue class often have that side effect is big, local excitation is strong, carcinogenic, preparation and use trouble or can not enough improve the drawbacks such as immunogenicity of poor antigen.Therefore, development novel immunostimulant efficient, low toxicity has become problem demanding prompt solution.Studies show that many Chinese medicines have immunological enhancement, possess advantage efficient, low toxicity, yet because traditional prescription of Chinese medicine mostly is big compound recipe, conventional dosage forms mostly is decoction, powder, administration inconvenience; And because its ingredient complexity is difficult to control of quality.
Three, summary of the invention
Technical problem the present invention is directed to the problem of being badly in need of efficient, low toxicity immunostimulant or immunological adjuvant in the anti-system of the current animal epidemic prior art, and a kind of novel compound Chinese medicine ingredients immunostimulant is provided, and possesses advantages such as safe, efficient, quality controllable.
A kind of novel compound ginseng-astragalus Chinese medicine ingredients immunostimulant of technical scheme the present invention abbreviates the ginseng stilbene as and increases and exempt from agent, it is characterized in that,
Every 1000ml medicinal liquid contains ginsengenin 20 .75g, astragalus polysaccharides 3.0g, and all the other are water for injection.
The technological process of producing every 1000ml medicinal liquid is: the ginsenoside is available from pharmaceutical factory, with TLC spectrophotometry content; Astragalus polysaccharides extracts with decoction alcohol precipitation method, measures content with sulphuric acid-anthrone method; Get the recipe quantity Chinese medicine ingredients by net content then and add the dissolving of injection water, fine straining, packing, autoclaving.
Beneficial effect
It is object of study that the present invention at first selects bibliographical information that astragalus polysaccharides (APS), epimedium brevicornum polysaccharide (EPS), propolis polysaccharide (PPS), propolis flavone (PF), ginsenoside's 10 kinds of Chinese medicine ingredients such as (GS) of enhance immunity effect are preferably arranged, extract preparation respectively, measure content, compare their enhance immunity effect and dose-effect relationship with cell culture, PCR and animal experiment method, filter out 5 kinds and act on Chinese medicine ingredients and optimal dose thereof preferably.Form a plurality of compound recipes respectively with 5 kinds of Chinese medicine ingredients, filter out effect 2 Chinese medicine ingredients compound recipes (side 2 exempts from agent for the ginseng stilbene increases) and optimal dose thereof preferably, and compare with the oily adjuvant of using always at present by serial animal experiment.The result shows, it is better that the ginseng stilbene increases the enhance immunity effect of exempting from agent, suitable with the effect of oily adjuvant, some time point in addition significantly be better than oily adjuvant (table 1～table 5, Fig. 1).
Further studied the ginseng stilbene and increased the mechanism of exempting from the agent enhance immunity, measured the influence of this side chickling periphery blood T lymphocyte IL-2mRNA and IFN-γ mRNA expression.The result shows, the ginseng stilbene increases the expression that the high dose of exempting from agent can significantly improve IL-2mRNA, and high, medium and low 3 dosage all can significantly improve expression (Fig. 2～Fig. 3) of IFN-γ mRNA.
Compare the ginseng stilbene by clinical trial and increased the immunoenhancement result of exempting from agent and corresponding Chinese medicine compound.The result shows, the ginseng stilbene increases exempts from agent and all can significantly promote the chickling lymphopoiesis and improve serum antibody titer that effect is suitable with oily adjuvant with corresponding Chinese medicine compound, increases the effect of exempting from agent and is better than corresponding Chinese medicine compound (table 6～table 7) slightly but join stilbene.
Measured the ginseng stilbene simultaneously and increased the safety of exempting from agent, prove that joining stilbene increases and exempt from agent man lagophthalmos conjunctiva and local muscle are not had any zest, LD50 is greater than 5000mg/kg, avirulence.
Compared with prior art, advantage of the present invention is as follows:
1. compare with similar chemical adjuvant, the ginseng stilbene increases that to exempt from agent not only reliable for effect, and derives from natural Chinese medicine, has no side effect, and no drug residue hidden danger meets the green medicine requirement, ensures animal-derived food safety; Produce the no three wastes, environmentally safe meets requirements of green environmental protection.
2. compare with traditional Chinese medicinal formulae, the ginseng stilbene increases that to exempt from agent prescription concise, dosage form advanced person, and quality improves, convenient drug administration.
3. compare with corresponding Chinese medicine compound, this prescription effect improves, and definite ingredients is quality controllable.The present invention with the cellular immunization and the humoral immune function that can significantly improve poultry, significantly promotes periphery blood T lymphocyte to transform, improve serum antibody titer and immune organ index as the immunostimulant list; , suitable as immunological adjuvant and vaccine with the effect of oily adjuvant with having enhance immunity effect preferably, at some time point even significantly be better than oily adjuvant, and be better than corresponding Chinese medicine compound; Pharmacological testing proves that said preparation can significantly promote lymphopoiesis, the expression of IL-2mRNA and IFN-γ mRNA in the raising chicken peripheral blood.
Four, description of drawings
Fig. 1. the ginseng stilbene increases exempts from the dynamic change that agent cooperates rabbit pestilence Seedling immunizing rabbit serum antibody titer.The ginseng stilbene increases exempts from that the agent group all is higher than matched group at the 3rd～21 day antibody titer after the immunity, the 17th day antibody titer is significantly higher than matched group after immunity; The a little higher than excessive honeybee of the 3rd, 10,17 day antibody titer increases and exempts from the agent group after immunity.
Fig. 2. the ginseng stilbene increases exempts from RT-PCR electrophoretogram (a) and the statistical result (b) that agent influences T lymphocyte IL-2mRNA expression.The ginseng stilbene increases exempts from the expression that agent high dose (cCHMIs2H) can significantly improve lymphocyte IL-2mRNA.
Fig. 3. the ginseng stilbene increases exempts from RT-PCR electrophoretogram (a) and the statistical result (b) that agent influences T lymphocyte IFN-γ mRNA expression.The ginseng stilbene increases the expression that 3 dosage (cCHMIs2H, cCHMIs2M, cCHMIs2L) of exempting from agent all can significantly improve lymphocyte IFN-γ mRNA.
Five, the specific embodiment
(1) screening of prescription and pharmacological effect are learned research
1. chickling immunity test
(1) prescription screening and with a plurality of compound recipes that relatively will form respectively of interleukin-2 immune synergism by astragalus polysaccharides, Radix Angelicae Sinensis polysaccharide, epimedium brevicornum polysaccharide, propolis flavone and the ginsenoside that campaign filters out, cooperates separately or with interleukin-2 and to inject 13 Japanese instar chicklings, for three days on end, in last injection, be the Seedling immunity with ND-IV, respectively after the immunity the 7th, 14,21,28 day every group randomly draw venous blood collection under 10 wings, detect the dynamic change of ND-HI antibody titer.The result shows that the effect of compound Chinese herbal medicinal ingredients enhance immunity effect is better than the single medicinal material composition, and the effect of APS-GS (ginseng stilbene increase exempt from agent) and EPS-PF (excessive honeybee increase exempt from agent) is better, can be used as candidate side's (table 1) of novel immunostimulant.
Table 1 Chinese medicine ingredients various combination and share influence (Log2) to chicken serum ND-HI antibody titer with interleukin-2
| Group | D 7 | D 14 | D 21 | D 28 |
| APS-GS APS-GS+IL-2 APS-PF APS-PF+IL-2 CAPS-GS CAPS-GS+IL-2 CAPS-PF CAPS-PF+IL-2 EPS-GS EPS-GS+IL-2 | 6.7±0.52 a 6.7±0.52 a 5.2±0.41 cd 5.0±0.00 de 5.7±0.52 bcd 5.2±0.75 cd 5.8±0.41 bc 5.5±0.55 bcd 5.7±0.52 bcd 5.2±0.41 cd | 7.3±0.52 abc 7.3±0.52 abc 6.3±0.52 e 6.3±0.52 e 6.5±0.55 de 7.0±0.00 bcd 6.6±0.55 cde 6.7±0.52 cde 6.7±0.52 cde 7.0±0.00 bcd | 6.2±0.75 ab 6.5±0.55 a 6.2±0.75 ab 6.0±0.82 abc 6.2±0.75 ab 6.5±0.55 a 5.8±0.41 abc 5.5±0.55 c 6.5±0.55 a 6.3±0.52 ab | 5.5±0.55 a 5.3±0.52 ab 5.0±0.63 ab 5.0±0.00 ab 4.8±0.75 abc 4.2±0.41 c 4.7±0.52 bc 4.5±0.22 c 4.8±0.41 abc 5.7±0.52 a |
| The contrast of EPS-PF EPS-PF+IL-2 GS-PF GS-PF+IL-2 APS CAPS EPS GS PF IL-2 vaccine | 6.0±0.00 b 5.5±0.52 bcd 5.5±0.55 bcd 5.5±0.55 bcd 5.5±0.55 bcd 5.3±0.52 bcd 4.5±0.55 e 5.3±0.52 bcd 5.7±0.52 bcd 5.0±0.00 de 4.5±0.55 e | 7.7±0.52 ab 7.8±0.41 a 6.5±0.55 de 7.2±0.75 bcd 6.3±0.52 e 5.2±0.41 f 6.8±0.41 cde 6.3±0.52 e 6.7±0.89 cde 6.5±0.55 de 6.3±0.52 e | 6.5±0.55 a 6.7±0.55 a 5.8±0.41 abc 5.7±0.52 bc 5.8±0.75 abc 5.5±0.55 c 6.3±0.52 ab 6.3±0.82 ab 5.7±0.41 bc 6.5±0.55 a 5.5±0.55 c | 5.5±0.55 a 5.0±0.63 ab 5.0±0.00 ab 4.7±0.52 bc 5.0±0.00 ab 5.2±0.52 ab 4.5±0.55 c 5.2±0.75 ab 4.7±0.52 bc 4.8±0.75 abc 4.2±0.41 c |
| Blank group | 3.3±0.55 f | 3.0±0.52 f | 2.8±0.55 d | 2.5±0.55 d |
(2) comparative test 1 of effect checking and dose-effect relationship thereof will join stilbene and increase and exempt from agent (side 2) and excessive honeybee and increase and exempt from agent (side 1) and divide 3 kinds of dosage to cooperate ND vaccine immunity chickling, and ( side 3,2 distinguishes the flavor of and makes up with other 2 Chinese medicine ingredients compound recipes; Side's 4,3 flavor combinations) and pure vaccine be contrast, the dynamic change of ND-HI antibody titer in the mensuration serum.3 dosage of test 2 general sides 1, side 2 reduce half respectively and cooperate ND inactivated vaccine immunized chicks, are contrast with oily adjuvant Seedling and propolis adjuvant Seedling, measure serum antibody titer and the exponential dynamic change of immune organ.The result confirms that it is certain that the ginseng stilbene increases the effect of exempting from agent, suitable with the effect of oily adjuvant, is better than propolis adjuvant, the effect of low dosage best (table 2～table 3).
The dynamic change (Log2) of serum N D-HI antibody titer is respectively organized in table 2 test 1
| Group | D 7 | D 14 | D 21 | D 28 |
| Side 1
High |
5.5±0.53 de 5.6±0.70 de 6.7±0.48 a 6.2±0.42 abc 5.7±0.82 cde 6.1±0.74 bcd 6.4±0.52 ab 5.7±0.48 cde 5.4±0.52 ef 5.3±0.48 efg 5.0±0.47 fg | 6.5±0.53 ab 7.0±0.82 a 7.1±0.74 a 7.2±0.79 a 7.0±0.74 a 6.9±0.88 a 6.8±0.63 a 6.7±0.48 a 6.8±0.88 a 6.6±0.52 ab 6.0±0.48 b | 6.3±0.48 bcd 6.4±0.52 abc 6.5±0.53 ab 6.3±0.48 bcd 6.4±0.52 abc 6.9±0.57 a 6.3±0.52 bcd 6.3±0.52 bcd 6.2±0.42 bcd 6.1±0.32 cd 5.8±0.42 d | 5.3±0.48 abc 5.7±0.48 a 5.6±0.52 a 5.3±0.48 abc 5.4±0.52 abc 5.5±0.53 ab 5.3±0.48 abc 5.5±0.53 ab 5.0±0.48 cd 5.1±0.42 bcd 4.7±0.48 de |
| Blank | 4.0±0.42 h | 3.8±0.48 c | 3.5±0.57 e | 3.0±0.48 f |
The dynamic change (Log2) of serum N D-HI antibody titer is respectively organized in table 3 test 2
| Group | One exempts from D 7 | One exempts from D 14 | One exempts from D 21 | Two exempt from D 7 | Two exempt from D 14 | Two exempt from D 21 |
| Side 1
High |
4.9±0.57 ab 5.2±0.63 a 4.7±0.48 ab 4.8±0.49 ab 5.0±0.67 ab 5.0±0.67 ab 4.6±0.52 b 4.6±0.52 b 4.5±0.52 b | 6.2±0.42 bc 6.5±0.54 ab 6.7±0.48 ab 6.2±0.42 bc 6.3±0.48 ab 6.6±0.54 ab 5.9±0.67 cd 6.4±0.52 ab 5.8±0.74 cd | 5.5±0.53 abc 6.0±0.47 a 5.9±0.48 ab 5.4±0.52 bc 5.3±0.48 bc 5.8±0.49 ab 5.7±0.52 ab 5.7±0.48 ab 5.2±0.78 c | 6.4±0.48 bc 6.9±0.42 a 6.7±0.53 ab 6.3±0.52 bc 6.7±0.53 ab 7.0±0.6 8a 6.5±0.67 ab 6.5±0.52 ab 6.0±0.57 c | 7.1±0.52 a7.1±0.52 a6.9±0.52 a6.9±0.52 a7.2±0.48 a7.2±0.38 a6.8±0.67 a7.1±0.69 a6.1±0.52 b | 5.6±0.52 bc 6.2±0.63 a 5.9±0.57 ab 5.5±0.53 bc 6.2±0.69 a 5.8±0.49 ab 5.6±0.52 bc 6.3±0.82 a 5.2±0.42 c |
| Blank | 3.8±0.71 c | 3.4±0.48 e | 3.2±0.52 d | 3.0±0.42 d | 2.7±0.52 c | 2.5±0.0 d |
In order further to screen optimal dose, 3 dosage of test 3 general sides 2, side 1 reduce half more respectively, be mixed and made into Chinese medicine adjuvant Seedling immunized chicks with newcastle disease vaccine virus, with no adjuvant Seedling and oily adjuvant Seedling is contrast, after immunity the 7th, 14,21,28,35, the 42d blood sampling, measure with mtt assay and micromethod respectively that periphery blood T lymphocyte transforms and the dynamic change of serum N D-HI antibody titer; Measure of the chicken spleen lymphopoietic influence of 2 compound recipes simultaneously to cultivating.The result shows, the ginseng stilbene increases exempts from agent and can significantly promote periphery blood T lymphocyte to transform, improve serum antibody titer, and suitable in the effect of most time point and oily adjuvant, the effect of middle dosage (former low dosage) is best, significantly is better than oily adjuvant at some time point; Significantly promote spleen lymphocyte proliferation (table 4～table 5) in certain concentration.
Variation (the OD that table 4 periphery blood T lymphocyte transforms
570)
| Group | D 7 | D 14 | D 21 | D 28 | D 35 |
| Side 1
High |
0.385±0.017 a 0.135±0.019 ef 0.113±0.005 f 0.185±0.037 d 0.318±0.033 b 0.255±0.042 c 0.165±0.034 de 0.158±0.009 de | 0.506±0.014 a0.391±0.036 b0.309±0.048 b0.334±0.079 b0.541±0.098 a0.376±0.090 b0.224±0.051 c0.228±0.033 c | 0.265±0.056 bc0.248±0.038 bcd0.214±0.096 cde0.286±0.043 b0.355±0.055 a0.248±0.066 bcd0.208±0.030 cde0.190±0.020 de | 0.215±0.037 bcd0.299±0.077 ab0.210±0.011 bcd0.24±0.015 bcd0.336±0.027 a0.270±0.097 abc0.194±0.058 bcd0.161±0.025 cd | 0.229±0.029 b0.188±0.018 bc0.200±0.053 bc0.334±0.091 a0.226±0.049 b0.206±0.044 bc0.216±0.042 bc0.166±0.029 c |
| Blank | 0.115±0.025 f | 0.160±0.030 c | 0.159±0.011 e | 0.154±0.036 d | 0.113±0.012 d |
The dynamic change of table 5 serum N D-HI antibody titer
| Group | D 7 | D 14 | D 21 | D 28 | D 35 | D 42 |
| Side 1
High |
5.2±0.48 a 5.0±0.47 ab 4.8±0.52 abc 4.5±0.53 bc 4.7±0.48 abc 4.6±0.52 bc 4.4±0.52 c 4.7±0.48 abc | 5.2±0.63 a5.1±0.57 a5.1±0.88 a4.4±0.52 b5.3±0.48 a5.1±0.50 a5.3±0.48 a4.5±0.53 b | 6.7±0.48 ab7.0±0.67 a5.8±0.42 cd5.6±0.69 d6.4±0.67 bc6.3±0.48 bc6.8±0.63 ab5.6±0.51 d | 6.2±0.48 ab6.0±0.53 abc5.7±0.63 bc5.9±0.53 abc5.8±0.48 abc5.8±0.48 abc6.3±0.63 a5.5±0.00 c | 5.8±0.42 a5.5±0.53 a4.8±0.42 c5.0±0.00 bc5.5±0.53 a5.0±0.67 bc5.4±0.52 ab5.0±0.00 bc | 5.5±0.55 a5.2±0.41 ab4.7±0.52 bc4.8±0.40 abc5.3±0.52 ab4.7±0.52 bc4.8±0.75 abc4.3±0.52 c |
| Contrast | 3.3±0.48 c | 3.3±0.67 c | 3.2±0.42 e | 3.0±0.42 d | 2.5±0.53 d | 2.4±0.54 d |
2. rabbit immunity test
Increase with the ginseng stilbene and to exempt from agent and excessive honeybee and increase and exempt from agent and cooperate rabbit pestilence inactivated vaccine immunizing rabbit respectively, matched group is a normal saline, measures the dynamic change of serum antibody titer after immunity on the the 3rd, 7,10,14,21,28,35,42 day with the hemagglutination inhibition test method.The result shows, the ginseng stilbene increases exempts from agent and excessive honeybee and increase and exempt from the serum antibody titer that agent all can significantly improve rabbit, and the ginseng stilbene increases exempts from agent some time point, especially early stage effect after immunity and be better than excessive honeybee and increase and exempt from agent (Fig. 1).
3. compound recipe strengthens the comparison that T lymphocyte IL-2mRNA expresses
In order to study the mechanism of compound Chinese herbal medicinal ingredients enhance immunity, extract total RNA from the chicken peripheral blood lymphocyte that ConA stimulates, amplify the IL-2 genetic fragment of 469bp, adopt sxemiquantitative RT-PCR method to measure the ginseng stilbene then and increase and exempt from agent and excessive honeybee and increase and exempt from the influence of agent chicken periphery blood T lymphocyte IL-2mRNA expression.The result shows that the ginseng stilbene increases exempts from the expression (Fig. 2) that the agent high dose can significantly improve lymphocyte IL-2mRNA.
4. compound recipe strengthens the comparison that T lymphocyte IFN-γ mRNA expresses
In order to study the mechanism of compound Chinese herbal medicinal ingredients enhance immunity, the a pair of interferon-primer of having used Primer Premier software design, from the total RNA of chicken peripheral blood lymphocyte that ConA stimulates, amplify the IFN-γ genetic fragment of 510bp, adopt sxemiquantitative PCR method to measure the ginseng stilbene then and increase and exempt from agent and excessive honeybee and increase and exempt from the influence of agent chicken peripheral blood lymphocyte IFN-γ mRNA expression.The result shows that the ginseng stilbene increases the expression (Fig. 3) that 3 dosage of exempting from agent all can significantly improve lymphocyte IFN-γ mRNA.
(2) with the comparison of Chinese medicine compound enhance immunity effect accordingly
Use epimedium brevicornum polysaccharide (EPS) to add propolis flavone (PF) respectively, ginsenoside (GS) adds astragalus polysaccharides (APS) and makes 2 Chinese medicine ingredients compound recipe (cCHMI
1, cCHMI
2), add propolis, people with corresponding Herba Epimedii simultaneously and participate in the extract of the Radix Astragali and make 2 identical Chinese medicine compound (cCHM of corresponding component content
1, cCHM
2).4 compound recipes are mixed and made into the vaccine immunity chickling with the strong poison of newcastle of deactivation respectively, with oily adjuvant Seedling (OA) and no adjuvant Seedling (NA) is contrast, respectively at the dynamic change that detected peripheral blood lymphocyte propagation and serum antibody titer after the immunity on the the 7th, 14,21,28,35,42 day with mtt assay and hemagglutination inhibition test method.The result shows that 4 compound recipes all can significantly promote chickling lymphopoiesis and raising serum antibody titer, and effect is suitable with oily adjuvant, but the effect of Chinese medicine ingredients compound recipe is better than Chinese medicine compound (table 6～table 7) slightly.
The lymphopoietic dynamic change of table 6 chicken
| Group | D 7 | D 14 | D 21 | D 28 | D 35 |
| cCHMI 1 cCHM 1 cCHMI 2 cCHM 2 OA NA | 0.135±0.019 b0.265±0.061 a0.318±0.033 a0.293±0.062 a0.165±0.034 b0.168±0.017 b | 0.391±0.036 b0.426±0.096 b0.541±0.099 a0.204±0.094 c0.224±0.051 d0.228±0.033 d | 0.246±0.070 b0.224±0.045 b0.355±0.055 a0.231±0.030 b0.183±0.045 c0.19±0.02 c | 0.299±0.077 ab0.236±0.045 bc0.313±0.068 a0.274±0.032 ab0.194±0.086 cd0.161±0.054 d | 0.188±0.018 a0.194±0.066 a0.216±0.049 a0.215±0.077 a0.216±0.042 a0.166±0.029 a |
| Control | 0.033±0.021 c | 0.16±0.031 d | 0.159±0.011 c | 0.154±0.051 d | 0.113±0.012 b |
The dynamic change of table 7. chicken serum antibody titer
| Group | D 7 | D 14 | D 21 | D 28 | D 35 | D 42 |
| cCHMI 1 cCHM 1 cCHMI 2 cCHM 2 OA NA | 5.0±0.47 a 4.3±0.48 b 4.7±0.48 ab 4.6±0.52 ab 4.4±0.52 b 4.7±0.48 ab | 5.1±0.57 a5.1±0.32 a5.3±0.48 a5.0±0.47 a5.3±0.48 a4.5±0.53 b | 7.0±0.67 a6.2±0.42 b6.4±0.67 ab6.2±0.63 b6.8±0.63 a5.6±0.52 c | 6.0±0.53 ab 5.5±0.47 ab 5.8±0.48 ab 5.9±0.52 ab 6.3±0.63 a 5.5±0.53 ab | 5.5±0.53 a5.6±0.52 a5.5±0.53 a5.3±0.48 ab5.4±0.52 ab5.0±0.00 b | 5.2±0.41 a4.3±0.52 b5.3±0.52 a5.2±0.41 a4.8±0.75 ab4.3±0.52 b |
| Control | 3.3±0.48 c | 3.3±0.67 c | 3.2±0.42 d | 3.0±0.42 c | 2.5±0.53 c | 2.4±0.54 c |
(3) safety research
Measured the ginseng stilbene with rabbit eye dripping method and quadriceps femoris injection and increased the local irritation of exempting from agent, measured the ginseng stilbene with the mouse peritoneal injection and increased the acute toxicity of exempting from agent, the result shows, joins stilbene and increases and exempt from agent man lagophthalmos conjunctiva and local muscle are not had any zest, LD
50Be 8435.29 ± 1379.28mg/kg, according to document, 5000mg/kg is above to be actual nontoxic, so said preparation is to the animal avirulence.
Embodiment: 1000ml compound ginseng-astragalus Chinese medicine ingredients immunostimulant medicinal liquid:
The ginsenoside is available from Jilin Ya Wei pharmaceutical factory, with the TLC spectrophotography (Liu Jianjian etc. the assay of Radix Astragali total saponins in the Radix Astragali injection. the Shanghai traditional Chinese medical science, 1997,7:33～34) measure content; The astragalus polysaccharides decoction and alcohol sedimentation technique (Liu Xiaoping etc. the research of astragalus polysaccharides oral liquid. Chinese crude drug, 1994,17 (6): 40～43) extract, with sulphuric acid-anthrone method (Zhao Wen etc. the Yi Li Radix Astragali and Radix Astagali composition relatively reach measurement of the polysaccharide content. Chinese herbal medicine, 2001,32 (2): 122～124) measure content; Get ginsengenin 20 .75g by net content then, astragalus polysaccharides 3.0g divides and adds the dissolving of injection water, fine straining, and packing, autoclaving obtains.
Evidence, the ginseng stilbene increases exempts from agent and uses separately as immunostimulant, can significantly promote lymphopoiesis, significantly improves the expression of chicken periphery blood T lymphocyte IL-2mRNA and IFN-γ mRNA; Cooperate rabbit pestilence Seedling immunizing rabbit as immunological adjuvant, the 3rd～21 day antibody titer all is higher than after immunity, the 17th day the antibody titer in immunity back is significantly higher than the matched group without adjuvant; Be mixed and made into Chinese medicine adjuvant Seedling immunized chicks with newcastle disease vaccine virus, with no adjuvant Seedling and oily adjuvant Seedling is contrast, can significantly promote the chickling periphery blood T lymphocyte to transform, improve serum antibody titer, suitable at most time point with the effect of oily adjuvant group, antibody titer at some time point is significantly higher than oily adjuvant group, also is better than the Chinese medicine compound made from the Radix Ginseng and the Radix Astragali.
Claims (2)
1. compound ginseng-astragalus Chinese medicine ingredients immunostimulant abbreviates the ginseng stilbene as and increases and exempt from agent, it is characterized in that every 1000ml medicinal liquid contains ginsengenin 20 .75g, astragalus polysaccharides 3.0g, and all the other are water for injection.
2. the preparation method of the described compound ginseng-astragalus Chinese medicine ingredients of claim 1 immunostimulant is characterized in that, the ginsenoside is available from pharmaceutical factory, with TLC spectrophotometry content; Astragalus polysaccharides extracts with decoction and alcohol sedimentation technique, measures content with sulphuric acid-anthrone method; Add the dissolving of injection water by net content weighting profit requirement 1 described recipe quantity Chinese medicine ingredients then, fine straining, packing, autoclaving obtains.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710019342 CN101019885A (en) | 2007-01-17 | 2007-01-17 | Compound ginseng-astragalus immunopotentiator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710019342 CN101019885A (en) | 2007-01-17 | 2007-01-17 | Compound ginseng-astragalus immunopotentiator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101019885A true CN101019885A (en) | 2007-08-22 |
Family
ID=38707811
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200710019342 Pending CN101019885A (en) | 2007-01-17 | 2007-01-17 | Compound ginseng-astragalus immunopotentiator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101019885A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101224220B (en) * | 2008-01-24 | 2010-04-07 | 上海中药创新研究中心 | Compounds for strengthening organism immunity function and application thereof |
| CN101554477B (en) * | 2009-03-17 | 2011-12-28 | 北京农学院 | Aftosa vaccine immunopotentiator |
| CN102988980A (en) * | 2011-09-14 | 2013-03-27 | 山东绿都生物科技有限公司 | Vaccine dilution liquid for enhancing hog cholera lapinized vaccine immunity effect, and its use method |
| CN103520219A (en) * | 2013-10-15 | 2014-01-22 | 胡艳欣 | Nature immunologic adjuvant and vaccine composition thereof, as well as preparation method |
| CN104069480A (en) * | 2014-06-11 | 2014-10-01 | 广州市华南农大生物药品有限公司 | Immunopotentiator and vaccine preparation for emergency vaccination of Newcastle disease |
| CN105963343A (en) * | 2016-05-14 | 2016-09-28 | 运城学院 | Compound radix codonopsis total saponins and immunopolysaccharides nano emulsion composition and preparation method thereof |
-
2007
- 2007-01-17 CN CN 200710019342 patent/CN101019885A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101224220B (en) * | 2008-01-24 | 2010-04-07 | 上海中药创新研究中心 | Compounds for strengthening organism immunity function and application thereof |
| CN101554477B (en) * | 2009-03-17 | 2011-12-28 | 北京农学院 | Aftosa vaccine immunopotentiator |
| CN102988980A (en) * | 2011-09-14 | 2013-03-27 | 山东绿都生物科技有限公司 | Vaccine dilution liquid for enhancing hog cholera lapinized vaccine immunity effect, and its use method |
| CN103520219A (en) * | 2013-10-15 | 2014-01-22 | 胡艳欣 | Nature immunologic adjuvant and vaccine composition thereof, as well as preparation method |
| CN103520219B (en) * | 2013-10-15 | 2019-05-07 | 胡艳欣 | A kind of innate immunity adjuvant and its vaccine composition and preparation method |
| CN104069480A (en) * | 2014-06-11 | 2014-10-01 | 广州市华南农大生物药品有限公司 | Immunopotentiator and vaccine preparation for emergency vaccination of Newcastle disease |
| CN105963343A (en) * | 2016-05-14 | 2016-09-28 | 运城学院 | Compound radix codonopsis total saponins and immunopolysaccharides nano emulsion composition and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kong et al. | Effects of Chinese herbal medicinal ingredients on peripheral lymphocyte proliferation and serum antibody titer after vaccination in chicken | |
| Guo et al. | Astragalus polysaccharide and sulfated epimedium polysaccharide synergistically resist the immunosuppression | |
| CN101019885A (en) | Compound ginseng-astragalus immunopotentiator | |
| CN101596246B (en) | Smoked plum extractive and wild jujube seed extractive compound preparation as well as preparation method and application thereof | |
| Jeney et al. | Plant extracts | |
| Yang et al. | The Taishan Robinia pseudoacacia polysaccharides enhance immune effects of rabbit haemorrhagic disease virus inactivated vaccines | |
| CN103288973A (en) | Isatis root polysaccharides, and preparation method and use thereof | |
| US11000569B2 (en) | Medicament for use in treating fatty liver, hepatitis and cirrhosis | |
| CN101002818A (en) | Yinfeng compound traditional Chinese medicine for enhancing inmmunity containing bee products | |
| CN101926861A (en) | Method for preparing Chinese medicinal veterinary medicament for enhancing non-specific immune function | |
| CN102133300A (en) | Chinese medicine composition with function of immunological enhancement and application thereof | |
| CN103860656A (en) | Traditional Chinese medicinal composition, and preparation method and application thereof | |
| CN108452300B (en) | Avian influenza inactivated vaccine adjuvant | |
| CN101912452A (en) | Veterinary Chinese medicinal composition for enhancing nonspecific immunologic function and application thereof | |
| CN101869590B (en) | Chinese medicinal herba epimedii and propolis flavone immunopotentiator | |
| CN101703772A (en) | Compound indigowoad root oral liquid for improving poultry immunity and preparation method thereof | |
| CN108553504A (en) | Join stilbene powder and preparation method thereof | |
| CN103142702A (en) | Chinese herbal medicine injection liquid for treating porcine reproductive and respiratory syndrome and preparation method thereof | |
| CN106995772A (en) | A kind of health liquor of strengthen immunity | |
| CN102973642B (en) | Chinese medicine composition for enhancing poultry immunity and preparation method thereof | |
| CN103263508B (en) | Traditional Chinese medicine composition for treating swine toxoplasmosis as well as preparation and application thereof | |
| CN106267186A (en) | A kind of have garlic polysaccharide and the compositions of selenizing Radix Codonopsis polysaccharide strengthening immunization | |
| CN104784327A (en) | Traditional Chinese veterinary medicine composition for treating respiratory diseases of chicken and preparation method thereof | |
| CN107007648B (en) | Immunity-enhancing traditional Chinese medicine composition and preparation method thereof | |
| CN104815052B (en) | A kind of Chinese medicine composition of resisiting influenza virus infection and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |