CN103141441A - Method for cultivating rotifers by using dried yeast and Antarctic krill meal - Google Patents
Method for cultivating rotifers by using dried yeast and Antarctic krill meal Download PDFInfo
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- CN103141441A CN103141441A CN2012104085866A CN201210408586A CN103141441A CN 103141441 A CN103141441 A CN 103141441A CN 2012104085866 A CN2012104085866 A CN 2012104085866A CN 201210408586 A CN201210408586 A CN 201210408586A CN 103141441 A CN103141441 A CN 103141441A
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- dried yeast
- south pole
- feeding
- krill powder
- wheel animalcule
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Abstract
The invention discloses a method for cultivating rotifers by using dried yeast and Antarctic krill meal. According to the invention, the dried yeast and the Antarctic krill meal are used as mixture bait for cultivating the rotifers. The method is characterized by comprising the steps of: carrying out superfine grinding on the Antarctic krill meal by using a cross-stirring grinder, and carrying out ultrasonic crush and dissolution on the Antarctic krill meal subjected to superfine grinding so as to enable the particle size of the Antarctic krill meal to be smaller than 25 mu m, thereby preparing the mother liquid of the Antarctic krill meal for later use; and inoculating the rotifers into a cement pit with the initial rotifer density being (550+/-10)inds/mL, water temperature being (25+/-0.5) DEG C and salt content being 28+/-1, continuously inflating area, and feeding the mixture bait, wherein the feeding quantity of the Antarctic krill meal is 50mg/L, the feeding quantity of the dried yeast is 0.4g/106inds, the dried yeast is dissolved by using fresh water and then is filtered by using 300-mesh bolting silk before being fed, and the Antarctic krill meal and the dried yeast are mixed according to the feeding quantities and are thrown into the cement pit together for feeding the rotifers.
Description
Technical field
The invention belongs to aquatic biological bait culture technique, particularly dried yeast and South Pole large krill powder and cultivate the method for wheel animalcule.
Background technology
Brachionus plicatilis (Brachionus plicatilis) (hereinafter to be referred as wheel animalcule) is a kind of small-sized multicellular aquatic animal, is distributed widely in the brackish water and body of sea water of temperature, torrid areas.It has suitable size, nutritious, breed the characteristics such as rapid, at seafood fish, crustaceanly grow seedlings the middle open-mouthed bait as young first-selection and be widely used.The yielding ability of wheel animalcule is cultivated the main extensive cultivation of native pond, the batch production cement pit of adopting and is cultivated and utilize the method such as bactogen High Density Cultivation.It is more traditional method that Tu Chi cultivates, general adopt ight soil or people's urine, chemical fertilizer, low value ocean fish etc. to cultivate micro-algae and bacterium as the bait of cultivating wheel animalcule as the source of manure, the wheel animalcule density obtained is lower, and easily be subject to the impact of the environmental conditions such as low temperature, rainfall and can't cultivate in a large number according to plan, due to the restriction that is subject to land resources, some area also can't be carried out wheel animalcule Tu Chi and be cultivated in addition; Utilize bactogen High Density Cultivation wheel animalcule, although there is the space of occupation of land few, the characteristics that wheel animalcule density is high that obtain, need higher equipment investment cost and production cost, complicated technique and and higher culture technique, therefore be difficult to applying in aquatic animal fry production.At present, wheel animalcule is cultivated the method basically adopt the batch production cement pit to cultivate, and this mode is mainly usingd micro-algae and yeast as the bait of cultivating wheel animalcule, and the deficiency of its existence is mainly manifested in the dependence of micro-algae larger, the wheel animalcule density of cultivating is lower, and the output of wheel animalcule is also unstable.Wheel animalcule bait is main, the direct factor that affects wheel animalcule breeding, in view of the supply of wheel animalcule and quantity thereof the importance for aquatic product animal raise seedling, needs badly and develops novel wheel animalcule bait, to improve existing wheel animalcule pattern for bait.
South Pole large krill (Euphausia superba) is subordinate to Arthropoda, Crustachia, Euphausiacea.South Pole large krill powder is rich in the nutriments such as protein, amino acid, fatty acid, vitamin, is a kind of bait of high-quality.Have no at present the relevant report that utilizes South Pole large krill powder to cultivate wheel animalcule.
Summary of the invention
A kind of method that the purpose of this invention is to provide dried yeast and South Pole large krill powder Mixed culture wheel animalcule, by using dried yeast and the South Pole large krill powder bait as wheel animalcule, improve culture density and the output of wheel animalcule, can meet fish, the shell-fish demand of the middle young to wheel animalcule of growing seedlings.
The mixed feed that technical scheme of the present invention adopts dried yeast and South Pole large krill powder to cultivate as wheel animalcule, it is characterized in that first using the intersection mixer grinder that South Pole large krill powder is carried out to ultramicro grinding, make the particle diameter of South Pole large krill powder<120 μ m, then join the proportioning of 600mL pure water through the South Pole of ultramicro grinding large krill powder by 100g, and stir with homogenizer, the rotating speed of homogenizer is 15500-22000rpm, mixing time is 2min, then by same procedure, repeatedly stir 3 times, adopt again ultrasonic disruption to dissolve 20min, make the particle diameter of South Pole large krill powder<25 μ m, make South Pole large krill powder mother liquor, and after with pure water, South Pole large krill powder mother liquor cumulative volume being complemented to 1L, be placed in 4 ℃ of refrigerators and store for future use, the step of cultivating wheel animalcule is that wheel animalcule is inoculated in cement pit, the wheel animalcule initial density is (550 ± 10) inds/mL, water temperature (25 ± 0.5) ℃, salinity 28 ± 1, continuous charge, in the South Pole large krill powder and dried yeast mixed feed of throwing something and feeding, the feeding volume of South Pole large krill powder is 50mg/L, be in the 1L seawater, the amount that applies South Pole large krill powder is 50mg, and the feeding volume of dried yeast is 0.4g/10
6inds, 10
6the inds wheel animalcule 0.4g dried yeast of throwing something and feeding, dried yeast is selected the Angel TH high activity dried yeast, and the front use fresh water of throwing something and feeding dissolves, then filters with 300 mesh sieve thin,tough silk, by above-mentioned feeding volume, after krill powder mother liquor and dried yeast are mixed, drops in the lump the cement pit wheel animalcule of feeding, respectively at the 12nd and 18h sampling, detect the wheel animalcule density of cultivating after throwing something and feeding.
Outstanding feature of the present invention is to cultivate wheel animalcule with dried yeast and South Pole large krill powder as mixed feed, and there is following remarkable technique effect: the one, the krill powder can promote the population growth of wheel animalcule, throw something and feed after South Pole large krill powder and dried yeast mixed feed, wheel animalcule density is significantly higher than the wheel animalcule density of the South Pole large krill powder of not throwing something and feeding, throw something and feed the 12nd and during 18h, the wheel animalcule density ratio that the mixed feed of throwing something and feeding is cultivated only throw something and feed the wheel animalcule density of dried yeast improved respectively nearly 11.84% and 15.08%(see attached list 1); The 2nd, South Pole large krill powder is of high nutritive value and aboundresources, and cost, far below micro-algae or concentrated micro-algae, has also reduced the man power and material that micro-algae is cultivated, and has been difficult for polluted water, does not also affect the wheel animalcule survival rate, and cultivating process adds simple to operate, easy to use.
Embodiment
The present invention uses dried yeast and the South Pole large krill powder mixed feed as wheel animalcule.South Pole large krill powder mother liquor preparation process is first to use the intersection mixer grinder that South Pole large krill powder is carried out to ultramicro grinding, for example select Pulverisette 16 types to intersect mixer grinders (German FRITSCH GmbH company produce) South Pole large krill powder is carried out to ultramicro grinding, make the particle diameter of South Pole large krill powder<120 μ m, then the proportioning that joins the 600mL pure water by 100g through the South Pole of ultramicro grinding large krill powder stirs with homogenizer, the rotating speed of homogenizer is 15500-22000rpm, mixing time is 2min, then by same procedure, repeatedly stir 3 times, adopt again ultrasonic disruption to dissolve 20min, make the particle diameter of South Pole large krill powder<25 μ m, make South Pole large krill powder mother liquor, and after with pure water, South Pole large krill powder mother liquor cumulative volume being complemented to 1L, be placed in 4 ℃ of refrigerators and store for future use, the step of cultivating wheel animalcule is that wheel animalcule is inoculated in cement pit, the wheel animalcule initial density is (550 ± 10) inds/mL, water temperature (25 ± 0.5) ℃, salinity 28 ± 1, continuous charge, the bait of throwing something and feeding is South Pole large krill powder and dried yeast (Angel TH high activity dried yeast) mixed feed, the feeding volume of South Pole large krill powder is that 50mg/L(is in the 1L seawater, the amount that applies South Pole large krill powder is 50mg), the feeding volume of dried yeast is 0.4g/10
6inds(is 10
6the inds wheel animalcule 0.4g dried yeast of throwing something and feeding), the dried yeast front use fresh water of throwing something and feeding dissolves, with 300 mesh sieve thin,tough silk, filter again, by above-mentioned feeding volume, by after krill powder mother liquor and dried yeast mixing, drop in the lump in cement pit the wheel animalcule of feeding, after throwing something and feeding, respectively at the 12nd and 18h sampling, measure the wheel animalcule density of cultivating.
The present invention is verified by following manner: the dried yeast of only throwing something and feeding in a cement pit, do not add South Pole large krill powder, and the feeding volume of dried yeast is 0.4g/10
6inds(is 10
6the inds wheel animalcule 0.4g dried yeast of throwing something and feeding), in another cement pit, throw something and feed South Pole large krill powder and dried yeast mixed feed, the feeding volume of South Pole large krill powder is that 50mg/L(is in the 1L seawater, and the amount that applies South Pole large krill powder is 50mg), the feeding volume of dried yeast is 0.4g/10
6inds(is 10
6the inds wheel animalcule 0.4g dried yeast of throwing something and feeding), in the 12nd and the 18h wheel animalcule density that adopts respectively Planktont counting frame measure to cultivate, measurement result shows, throws something and feeds after dried yeast and South Pole large krill powder mixed feed, and wheel animalcule density is significantly higher than the wheel animalcule density of the South Pole large krill powder of not throwing something and feeding.The 12nd and during 18h, the wheel animalcule density ratio that the mixed feed of throwing something and feeding is cultivated only throw something and feed the wheel animalcule density of dried yeast improved respectively nearly 11.84% and 15.08%(see attached list 1).
The different bait different time points of subordinate list 1. wheel animalcule density measurement data (inds/mL)
Claims (1)
1. a dried yeast and South Pole large krill powder are cultivated the method for wheel animalcule, the mixed feed that technical scheme of the present invention adopts dried yeast and South Pole large krill powder to cultivate as wheel animalcule, it is characterized in that first using the intersection mixer grinder that South Pole large krill powder is carried out to ultramicro grinding, make the particle diameter of South Pole large krill powder<120 μ m, then join the proportioning of 600mL pure water through the South Pole of ultramicro grinding large krill powder by 100g, and stir with homogenizer, the rotating speed of homogenizer is 15500-22000rpm, mixing time is 2min, then by same procedure, repeatedly stir 3 times, adopt again ultrasonic disruption to dissolve 20min, make the particle diameter of South Pole large krill powder<25 μ m, make South Pole large krill powder mother liquor, and after with pure water, South Pole large krill powder mother liquor cumulative volume being complemented to 1L, be placed in 4 ℃ of refrigerators and store for future use, the step of cultivating wheel animalcule is that wheel animalcule is inoculated in cement pit, the wheel animalcule initial density is (550 ± 10) inds/mL, water temperature (25 ± 0.5) ℃, salinity 28 ± 1, continuous charge, in the South Pole large krill powder and dried yeast mixed feed of throwing something and feeding, the feeding volume of South Pole large krill powder is 50mg/L, be in the 1L seawater, the amount that applies South Pole large krill powder is 50mg, and the feeding volume of dried yeast is 0.4g/10
6inds, 10
6the inds wheel animalcule 0.4g dried yeast of throwing something and feeding, dried yeast is selected the Angel TH high activity dried yeast, and the front use fresh water of throwing something and feeding dissolves, then filters with 300 mesh sieve thin,tough silk, by above-mentioned feeding volume, after krill powder mother liquor and dried yeast are mixed, drops in the lump the cement pit wheel animalcule of feeding, respectively at the 12nd and 18h sampling, detect the wheel animalcule density of cultivating after throwing something and feeding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210408586.6A CN103141441B (en) | 2012-10-23 | 2012-10-23 | Method for cultivating rotifers by using dried yeast and Antarctic krill meal |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210408586.6A CN103141441B (en) | 2012-10-23 | 2012-10-23 | Method for cultivating rotifers by using dried yeast and Antarctic krill meal |
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| Publication Number | Publication Date |
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| CN103141441A true CN103141441A (en) | 2013-06-12 |
| CN103141441B CN103141441B (en) | 2014-06-04 |
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| CN201210408586.6A Expired - Fee Related CN103141441B (en) | 2012-10-23 | 2012-10-23 | Method for cultivating rotifers by using dried yeast and Antarctic krill meal |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104719207A (en) * | 2015-03-24 | 2015-06-24 | 临沂大学 | Method for cultivating branchionus plicatilis through waste plastic buckets |
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| JP2004057206A (en) * | 2002-06-07 | 2004-02-26 | Ikeda Shokken Kk | Feed |
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- 2012-10-23 CN CN201210408586.6A patent/CN103141441B/en not_active Expired - Fee Related
Patent Citations (8)
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| US20090317424A1 (en) * | 2001-09-05 | 2009-12-24 | Huey-Lang Yang | Oral vaccines |
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| CN102273431A (en) * | 2011-06-01 | 2011-12-14 | 深圳大学 | Method for co-culturing freshwater rotifers and chlorella vulgaris |
| CN102511432A (en) * | 2012-01-13 | 2012-06-27 | 广东海洋大学 | Method for cultivating Brachionus plicatilis |
| CN102599109A (en) * | 2012-02-29 | 2012-07-25 | 谢达祥 | Simple method for purifying rotifers in batch |
| CN102630645A (en) * | 2012-04-23 | 2012-08-15 | 天津海友佳音生物科技股份有限公司 | Method for culturing ss (super-small) type branchionus plicatilis by utilizing high-concentration fresh water chlorella |
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| CN104719207A (en) * | 2015-03-24 | 2015-06-24 | 临沂大学 | Method for cultivating branchionus plicatilis through waste plastic buckets |
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| CN103141441B (en) | 2014-06-04 |
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