CN102511432A - Method for cultivating Brachionus plicatilis - Google Patents
Method for cultivating Brachionus plicatilis Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 21
- 241000700104 Brachionus plicatilis Species 0.000 title claims description 12
- 241000700141 Rotifera Species 0.000 claims abstract description 60
- 241000251468 Actinopterygii Species 0.000 claims abstract description 45
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 43
- 238000009395 breeding Methods 0.000 claims abstract description 9
- 230000001488 breeding effect Effects 0.000 claims abstract description 9
- 230000032683 aging Effects 0.000 claims abstract description 3
- 238000005276 aerator Methods 0.000 claims description 12
- 239000013505 freshwater Substances 0.000 claims description 12
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 8
- 239000000292 calcium oxide Substances 0.000 claims description 4
- 235000012255 calcium oxide Nutrition 0.000 claims description 4
- 238000004061 bleaching Methods 0.000 claims description 2
- 238000000855 fermentation Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 claims 1
- 238000001914 filtration Methods 0.000 claims 1
- 238000009434 installation Methods 0.000 claims 1
- 238000007726 management method Methods 0.000 claims 1
- 238000004659 sterilization and disinfection Methods 0.000 claims 1
- 241000700106 Brachionus Species 0.000 abstract description 63
- 241000698291 Rugosa Species 0.000 abstract description 59
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract description 2
- 230000035558 fertility Effects 0.000 abstract description 2
- 230000007774 longterm Effects 0.000 abstract description 2
- 238000006213 oxygenation reaction Methods 0.000 abstract 2
- 239000010813 municipal solid waste Substances 0.000 description 15
- 230000005484 gravity Effects 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 7
- 241000195493 Cryptophyta Species 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 230000002354 daily effect Effects 0.000 description 5
- 230000003247 decreasing effect Effects 0.000 description 5
- 238000012136 culture method Methods 0.000 description 4
- 230000003203 everyday effect Effects 0.000 description 4
- 230000000243 photosynthetic effect Effects 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 2
- 241001282110 Pagrus major Species 0.000 description 2
- 239000007844 bleaching agent Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000700105 Brachionidae Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000242583 Scyphozoa Species 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
本发明公开了一种褶皱臂尾轮虫的培养方法，属于水产养殖技术领域。本发明褶皱臂尾轮虫的培养方法是利用废弃或老化的旧池塘作为培养池塘，以小杂鱼作为基础饵料对皱臂尾轮虫进行培养，具体步骤是将培养池塘经消毒、暴晒处理后进水，安装增氧设备，然后将基础饵料按一定量以网袋盛装吊挂与培养池塘水中，在日常管理中结合增氧设备培养13-18天，当轮虫密度达到250个/mL，开始用褶皱臂尾轮虫收集袋收集，并根据褶皱臂尾轮虫的繁育速度和密度追加小杂鱼的吊挂量。本发明褶皱臂尾轮虫的培养方法简单方便，培养的轮虫密度大、营养全面，而且水中肥力长久，不用频繁投饵，可以大幅度降低生产成本，提高经济效益。The invention discloses a method for cultivating Brachionus rugosa, belonging to the technical field of aquaculture. The method for cultivating Brachionus rugosa of the present invention is to use abandoned or aging old ponds as the cultivation pond, and use small miscellaneous fish as the basic bait to cultivate the Brachionus rugosa rotifer, and the specific steps are to disinfect and expose the cultivation pond to the sun Enter the water, install the oxygenation equipment, and then hang the basic bait in a mesh bag according to a certain amount and cultivate it in the pond water. In the daily management, combine the oxygenation equipment to cultivate for 13-18 days. When the rotifer density reaches 250/mL, Begin to collect with the collection bag of Brachionus rugosa, and increase the hanging amount of small miscellaneous fish according to the breeding speed and density of Brachionus rugosa. The method for cultivating the Brachionus rugosa is simple and convenient, the cultured rotifers have high density, comprehensive nutrition, long-term fertility in water, no need for frequent baiting, can greatly reduce production costs, and improve economic benefits.
Description
技术领域 technical field
本发明涉及海洋经济动物苗种培育过程中一种优良生物饵料的培养方法，特别是一种褶皱臂尾轮虫的培养方法，属于水产养殖技术领域。 The invention relates to a method for cultivating high-quality biological bait in the seedling cultivation process of marine economic animals, in particular to a method for cultivating Brachionus rugosa rotifers, and belongs to the technical field of aquaculture.
背景技术 Background technique
褶皱臂尾轮虫(Brachionus plicatilis)属轮虫纲、单巢目、游泳亚目、臂尾轮虫科，它具有广温和广盐性、对环境适应能力强、生长繁殖速度快、大小适宜（100μm～300μm）及营养丰富等特点，已成为鱼、虾、蟹和海蜇等重要海洋经济动物苗种培育的优良生物饵料。海产鱼类的苗种孵出时一般较小，除少数种类外，它们的体长大约在2～7mm之间，而开口摄食时其口径更小，因此，投喂营养丰富、大小适口的开口饵料，是鱼类人工育苗成败的关键。轮虫应用于海产鱼类苗种生产始于20世纪60年代末、70年代初，日本学者首先在真鲷(Pagrus major)的苗种规模化生产中应用褶皱臂尾轮虫获得成功，之后轮虫便成为所有海产鱼类苗种生产的通用饵料生物，迄今为止，用轮虫作为开口饵料成功地进行苗种生产的海产经济鱼类已达60 余种。 Brachionus plicatilis belongs to the class Rotifera, Monoster, Swimming suborder, Brachionidae, it has wide temperature and wide salt, strong adaptability to the environment, fast growth and reproduction speed, suitable size ( 100μm ~ 300μm) and rich in nutrients, it has become an excellent biological bait for seedling cultivation of important marine economic animals such as fish, shrimp, crab and jellyfish. The seedlings of marine fish are generally small when hatched. Except for a few species, their body length is about 2-7mm, and their caliber is smaller when they open their mouths to feed. The bait is the key to the success or failure of fish artificial breeding. The application of rotifers to the production of marine fish seedlings began in the late 1960s and early 1970s. Japanese scholars first applied Brachionus rugosa successfully in the large-scale production of red sea bream (Pagrus major). Rotifers have just become the general bait organisms for the production of all marine fish fry. So far, the marine economic fishes that have successfully carried out seed production with rotifers as open bait have reached more than 60 species.
轮虫食性广泛，主要摄食水中的细小物质，单胞藻、细菌、酵母，甚至豆浆、发酵鸡粪浆、草浆等有机碎屑均可作为其培育饵料。传统的轮虫培养，一般采用单胞藻、细菌或酵母作为其饵料。虽然小球藻、扁藻等单胞藻和光合细菌等细菌培育轮虫营养全面，饵料效果好，但是，由于受单胞藻和光合细菌培育场地的限制，在进行大规模轮虫培育时，单胞藻和光合细菌的量很难做到足量供给；其次，由于轮虫食量大，用单胞藻和光合细菌培育轮虫，一般情况下轮虫密度很难达到200个/mL以上。因此，实际生产中，要进行大规模的高密度轮虫培育，常用酵母作为轮虫培育的饵料，但用酵母培养的轮虫缺乏鱼类生长的不饱和脂肪酸，轮虫投喂时须经短时间的单胞藻或乌贼肝油等脂类营养强化，而且在室内水泥池培养时投喂酵母水质难以控制，培养成本高。因此，采用设施简陋、生产成本低的方法培养轮虫，可以实现经济效益的最大化。 Rotifers have a wide range of feeding habits, and mainly feed on small substances in the water. Unicellular algae, bacteria, yeast, and even organic debris such as soybean milk, fermented chicken manure, and grass pulp can be used as bait for their cultivation. Traditional rotifer culture generally adopts single cell algae, bacteria or yeast as its bait. Although bacteria such as chlorella, flat algae and photosynthetic bacteria cultivate rotifers with comprehensive nutrition and good bait effect, but due to the limitation of the breeding sites of unicellular algae and photosynthetic bacteria, when carrying out large-scale rotifer cultivation, The amount of unicellular algae and photosynthetic bacteria is difficult to achieve a sufficient supply; secondly, due to the large food intake of rotifers, the rotifers are cultivated with unicellular algae and photosynthetic bacteria, and the rotifer density is generally difficult to reach more than 200/mL. Therefore, in actual production, it is necessary to carry out large-scale high-density rotifer cultivation, and yeast is often used as the bait for rotifer cultivation, but the rotifers cultivated with yeast lack the unsaturated fatty acids for fish growth, and the rotifers must be fed after a short period of time. In addition, it is difficult to control the water quality of the yeast when it is cultivated in an indoor cement tank, and the cultivation cost is high. Therefore, cultivating rotifers with poor facilities and low production costs can maximize economic benefits.
发明内容 Contents of the invention
本发明目的在于提供一种褶皱臂尾轮虫的培养方法，该方法操作简单方便，可实现大规模的高密度褶皱臂尾轮虫的培育，且生产成本低，经济效益高。 The purpose of the present invention is to provide a method for cultivating Brachionus rugosa, which is simple and convenient to operate, can realize large-scale high-density cultivation of Brachionus rugosa, and has low production cost and high economic benefits.
为解决上述问题，本发明所采用的技术方案是：一种褶皱臂尾轮虫的培养方法包括如下步骤： In order to solve the above problems, the technical solution adopted in the present invention is: a method for cultivating Brachionus rugosa rotifers comprises the steps:
（1）培养设施：选择淡水来源方便、面积667.7～1000m2、深1.0～1.2m的废弃或老化的旧池塘作为培养池塘，在褶皱臂尾轮虫培养前用120-150kg/亩的生石灰或4-5kg/亩的漂白精对培养池塘进行消毒、暴晒一个星期后，用100目的筛绢过滤进水至培养池塘中水深0.8～1.0m，在培养池塘对角安装二台功率为0.75kv的水车式增氧机； (1) Cultivation facilities: Choose an abandoned or aging old pond with a convenient source of fresh water, an area of 667.7-1000m 2 , and a depth of 1.0-1.2m as the cultivation pond, and use 120-150kg/mu of quicklime or Disinfect the cultivation pond with 4-5kg/mu bleaching essence and expose it to the sun for a week, then filter the incoming water with a 100-mesh sieve until the water depth in the cultivation pond is 0.8-1.0m, and install two sets of 0.75kv power generators at the opposite corners of the cultivation pond. Water wheel aerator;
（2）基础饵料：以小杂鱼为培养褶皱臂尾轮虫的基础饵料，小杂鱼用量为180～200kg/亩，将小杂鱼按15-20kg/袋，用80目的网袋盛装，然后扎紧网袋袋口，将装有小杂鱼的网袋均匀地吊挂于培养池塘的水中； (2) Basic bait: Use small trash fish as the basic bait for cultivating Brachionus rugosa, the amount of small trash fish is 180-200kg/mu, and the small trash fish is 15-20kg/bag and packed in 80-mesh mesh bags. Then tie up the mouth of the net bag, and evenly hang the net bag with small miscellaneous fish in the water of the cultivation pond;
（3）日常管理：利用淡水调节培养池塘塘水比重，使培养池塘塘水比重维持在1.010～1.015之间；小杂鱼在培养池塘塘水中8-12天开始腐烂发酵，此时每天上午8时、下午6时开动增氧机，每次开动0.5-1.5小时，培养13-18天；当褶皱臂尾轮虫的密度达到250个/mL时，开始用轮虫收集袋收集褶皱臂尾轮虫；收集至观测得培养池塘中褶皱臂尾轮虫的繁育速度下降、密度明显降低时及时追加吊挂小杂鱼，追加量为40～50kg/亩。 (3) Daily management: use fresh water to adjust the specific gravity of the water in the cultivation pond, so that the specific gravity of the cultivation pond water is maintained between 1.010 and 1.015; Start the aerator at 6:00 p.m. for 0.5-1.5 hours each time, and cultivate for 13-18 days; when the density of Brachionus rugosa reaches 250/mL, start to collect Brachionus rugosa with a rotifer collection bag When the breeding speed of Brachionus rugosa in the culture ponds was collected and observed, and the density decreased significantly, small miscellaneous fish were suspended in time, and the additional amount was 40-50kg/mu.
所述步骤（3）中的轮虫收集袋为用100目筛绢制作而成且两端开口的圆形的100目筒形袋和用250目的筛绢制作而成且两端开口的圆形的250目筒形袋：100目筒形袋长5m、直径30cm，用于过滤掉较大的物体；250目筒形袋长5.5m、直径50cm，用于收集轮虫。 The rotifer collection bag in the step (3) is a circular 100-mesh cylindrical bag made of 100-mesh sieve with openings at both ends and a circular 100-mesh cylindrical bag made with 250-mesh sieve and both ends are open. The 250-mesh cylindrical bag: the 100-mesh cylindrical bag is 5m long and 30cm in diameter, used to filter out larger objects; the 250-mesh cylindrical bag is 5.5m long and 50cm in diameter, used to collect rotifers.
所述步骤（3）中用轮虫收集袋收集褶皱臂尾轮虫的方法如下：收集时，将250目筒形袋套在100目筒形袋的外面，将两个轮虫收集袋的一端开口分别扎紧，另一端开口均绑在潜水泵的出水口处。 In the step (3), the method of collecting Brachionus rugosa with a rotifer collection bag is as follows: when collecting, put a 250-mesh cylindrical bag on the outside of a 100-mesh cylindrical bag, and wrap one end of the two rotifer collection bags The openings are fastened respectively, and the openings at the other end are tied to the water outlet of the submersible pump.
所述潜水泵用木桩固定在离培养池塘底部高50cm处。 The submersible pump is fixed on a place 50 cm above the bottom of the cultivation pond with stakes.
本发明相对于现有技术的有益效果是：本发明是根据褶皱臂尾轮虫的生活、摄食及繁殖特性，利用废弃或利用价值不大的旧池塘，以小杂鱼为基础饵料，同时还配套增氧设备，进行褶皱臂尾轮虫的培养，本发明所述褶皱臂尾轮虫培养方法，改进了轮虫传统培养中需经常投喂饵料的繁锁，操作简单方便，所培养的褶皱臂尾轮虫密度大（250个/mL以上）、营养全面，不需要用单胞藻或其他物质进行强化培养就可以直接投喂种苗，而且水中肥力长久，满足轮虫长时间生长、繁殖所需的营养物质，从而降低了生产成本，经济效益显著提高。 The beneficial effects of the present invention relative to the prior art are: the present invention is based on the life, feeding and reproduction characteristics of Brachionus rugosa, utilizes old ponds that are abandoned or have little utilization value, and uses small miscellaneous fish as the basic bait. Supporting aeration equipment for the cultivation of Brachionus rugosa, the method for cultivating Brachionus rugosa in the present invention improves the cumbersomeness of feeding bait frequently in the traditional cultivation of rotifers, the operation is simple and convenient, and the cultured rotifers Brachionus rotifers have a high density (above 250/mL) and comprehensive nutrition. They can be directly fed to seedlings without intensive cultivation with unicellular algae or other substances. Moreover, the fertility in the water is long-lasting, which satisfies the long-term growth and reproduction of rotifers. required nutrients, thereby reducing production costs and significantly improving economic benefits.
具体实施方式 Detailed ways
下面通过实施例对本发明做进一步详细说明，这些实施例仅用来说明本发明，并不限制本发明的范围。 The present invention will be described in further detail below through examples, and these examples are only used to illustrate the present invention, and do not limit the scope of the present invention.
实施例1 采用本发明的褶皱臂尾轮虫培养方法，按照下列步骤培养褶皱臂尾轮虫： Embodiment 1 adopts Brachionus rugosa culture method of the present invention, cultivates Brachionus rugosa according to the following steps:
（1）培养设施：选择淡水来源方便、面积667.7m2、深1.2m的废弃旧池塘作为培养池塘，在褶皱臂尾轮虫培养前用150kg/亩的生石灰对培养池塘进行消毒、暴晒一个星期后，用100目的筛绢过滤进水至培养池塘中水深0.85m，在培养池塘对角安装二台功率为0.75kv的水车式增氧机； (1) Cultivation facilities: choose an abandoned old pond with a convenient source of fresh water, an area of 667.7m 2 , and a depth of 1.2m as the cultivation pond. Before the cultivation of Brachionus rugosa, the cultivation pond is disinfected with 150kg/mu of quicklime and exposed to the sun for one week Finally, filter the incoming water with a 100-mesh sieve to a water depth of 0.85m in the cultivation pond, and install two waterwheel type aerators with a power of 0.75kv at the diagonal of the cultivation pond;
（2）基础饵料：以小杂鱼为培养褶皱臂尾轮虫的基础饵料，小杂鱼用量为188kg/亩，将小杂鱼按15kg/袋，用80目的网袋盛装，然后扎紧网袋袋口，将装有小杂鱼的网袋均匀地吊挂于培养池塘的水中； (2) Basic bait: Use small trash fish as the basic bait for cultivating Brachionus rugosa, the amount of small trash fish is 188kg/mu, put the small trash fish in 15kg/bag, put them in 80-mesh mesh bags, and then tie the net tightly At the mouth of the bag, evenly hang the net bag with small miscellaneous fish in the water of the cultivation pond;
（3）日常管理：利用淡水调节培养池塘塘水比重，使培养池塘塘水比重维持在1.010；小杂鱼在培养池塘塘水中8天开始腐烂发酵，此时每天上午8时、下午6时开动增氧机，每次开动0.5小时，培养15天，当褶皱臂尾轮虫的密度达到250个/mL时，开始轮虫收集袋收集褶皱臂尾轮虫； (3) Daily management: use fresh water to adjust the specific gravity of the water in the cultivation pond, so that the specific gravity of the cultivation pond water is maintained at 1.010; small miscellaneous fish begin to rot and ferment in the water of the cultivation pond for 8 days, and start at 8:00 am and 6:00 pm every day Aerator, start 0.5 hour each time, cultivate 15 days, when the density of Brachionus rugosa reaches 250/mL, start the rotifer collection bag to collect Brachionus rugosa;
（4）轮虫收集： (4) Rotifer collection:
1）准备两个轮虫收集袋：用100目筛绢制作而成长5m、直径30cm且两端开口的圆形的100目筒形袋和用250目的筛绢制作而成长5.5m、直径50cm的250目筒形袋； 1) Prepare two rotifer collection bags: a circular 100-mesh cylindrical bag made of 100-mesh sieve silk with a length of 5m, a diameter of 30cm and openings at both ends, and a rotifer bag made of 250-mesh sieve with a length of 5.5m and a diameter of 50cm 250 mesh tubular bag;
2）轮虫收集方法：将250目筒形袋套在100目筒形袋的外面，将两个轮虫收集袋的一端开口分别扎紧，另一端开口均绑在用木桩固定在离培养池塘底部高50cm处的潜水泵的出水口； 2) The method of collecting rotifers: Put a 250-mesh cylindrical bag on the outside of a 100-mesh cylindrical bag, tie the openings at one end of the two rotifer collection bags tightly respectively, and tie the openings at the other end to a wooden stake and fix it in a separate culture area. The outlet of the submersible pump at a height of 50 cm from the bottom of the pond;
3）收集至250目筒形袋中褶皱臂尾轮虫的数量已较多，取下两个轮虫收集袋，从250目筒形袋中收取褶皱臂尾轮虫； 3) The number of Brachionus rugosa collected in the 250-mesh cylindrical bag is already large, remove the two rotifer collection bags, and collect the Brachionus rugosa from the 250-mesh cylindrical bag;
4）收集至观测到培养池塘中褶皱臂尾轮虫的繁育速度下降、褶皱臂尾轮虫密度已降低至100个/mL，表明池塘中的营养物质已满足不了褶皱臂尾轮虫生长的需要，因此追加小杂鱼的吊挂，追加量为48kg/亩。 4) From collection to observation, the breeding speed of Brachionus rugosa in the culture pond has decreased, and the density of Brachionus rugosa has dropped to 100/mL, indicating that the nutrients in the pond can no longer meet the growth needs of Brachionus rugosa , so add the hanging of small miscellaneous fish, the additional amount is 48kg/mu.
实施例2 采用本发明的褶皱臂尾轮虫培养方法，按照下列步骤培养褶皱臂尾轮虫： Embodiment 2 adopts Brachionus rugosa culture method of the present invention, cultivates Brachionus rugosa according to the following steps:
（1）培养设施：选择淡水来源方便、面积826m2、深1.1m的废弃旧池塘作为培养池塘，在褶皱臂尾轮虫培养前用5kg/亩的漂白精对培养池塘进行消毒、暴晒一个星期后，用100目的筛绢过滤进水至培养池塘中水深1.0m，在培养池塘对角安装二台功率为0.75kv的水车式增氧机； (1) Cultivation facilities: choose an abandoned old pond with a convenient source of fresh water, an area of 826m 2 , and a depth of 1.1m as the cultivation pond. Before the cultivation of Brachionus rugosa, the cultivation pond is disinfected with 5kg/mu of bleach and exposed to the sun for a week Finally, filter the incoming water with a 100-mesh sieve to a water depth of 1.0m in the cultivation pond, and install two waterwheel type aerators with a power of 0.75kv at the diagonal of the cultivation pond;
（2）基础饵料：以小杂鱼为培养褶皱臂尾轮虫的基础饵料，小杂鱼用量为200kg/亩，将小杂鱼按17kg/袋，用80目的网袋盛装，然后扎紧网袋袋口，将装有小杂鱼的网袋均匀地吊挂于培养池塘的水中； (2) Basic bait: Use small trash fish as the basic bait for cultivating Brachionus rugosa, the amount of small trash fish is 200kg/mu, put the small trash fish in 17kg/bag, put them in 80-mesh mesh bags, and then tie the net tightly At the mouth of the bag, evenly hang the net bag with small miscellaneous fish in the water of the cultivation pond;
（3）日常管理：利用淡水调节培养池塘塘水比重，使培养池塘塘水比重维持在1.013；小杂鱼在培养池塘塘水中10天开始腐烂发酵，此时每天上午8时、下午6时开动增氧机，每次开动1.2小时，培养13天，当褶皱臂尾轮虫的密度达到250个/mL时，开始轮虫收集袋收集褶皱臂尾轮虫； (3) Daily management: Use fresh water to adjust the specific gravity of the water in the cultivation pond, so that the specific gravity of the cultivation pond water is maintained at 1.013; the small miscellaneous fish begin to rot and ferment in the water of the cultivation pond for 10 days, and start at 8:00 a.m. and 6:00 p.m. every day Aerator, start 1.2 hours each time, cultivate 13 days, when the density of Brachionus rugosa reaches 250/mL, start the rotifer collection bag to collect Brachionus rugosa;
（4）轮虫收集： (4) Rotifer collection:
1）准备两个轮虫收集袋：用100目筛绢制作而成长5m、直径30cm且两端开口的圆形的100目筒形袋和用250目的筛绢制作而成长5.5m、直径50cm的250目筒形袋； 1) Prepare two rotifer collection bags: a circular 100-mesh cylindrical bag made of 100-mesh sieve silk with a length of 5m, a diameter of 30cm and openings at both ends, and a rotifer bag made of 250-mesh sieve with a length of 5.5m and a diameter of 50cm 250 mesh tubular bag;
2）轮虫收集方法：将250目筒形袋套在100目筒形袋的外面，将两个轮虫收集袋的一端开口分别扎紧，另一端开口均绑在用木桩固定在离培养池塘底部高50cm处的潜水泵的出水口； 2) The method of collecting rotifers: Put a 250-mesh cylindrical bag on the outside of a 100-mesh cylindrical bag, tie the openings at one end of the two rotifer collection bags tightly respectively, and tie the openings at the other end to a wooden stake and fix it in a separate culture area. The outlet of the submersible pump at a height of 50 cm from the bottom of the pond;
3）收集至250目筒形袋中褶皱臂尾轮虫的数量已较多，取下两个轮虫收集袋，从250目筒形袋中收取褶皱臂尾轮虫； 3) The number of Brachionus rugosa collected in the 250-mesh cylindrical bag is already large, and the two rotifer collection bags are removed, and the Brachionus rugosa is collected from the 250-mesh cylindrical bag;
4）收集至观测到培养池塘中褶皱臂尾轮虫的繁育速度下降、褶皱臂尾轮虫密度已降低至80个/mL，表明池塘中的营养物质已满足不了褶皱臂尾轮虫生长的需要，因此追加小杂鱼的吊挂，追加量为50kg/亩。 4) From collection to observation, the breeding speed of Brachionus rugosa in the culture pond has decreased, and the density of Brachionus rugosa has dropped to 80/mL, indicating that the nutrients in the pond can no longer meet the growth needs of Brachionus rugosa , so the hanging of small miscellaneous fish is added, and the additional amount is 50kg/mu.
实施例3 采用本发明的褶皱臂尾轮虫培养方法，按照下列步骤培养褶皱臂尾轮虫： Embodiment 3 adopts Brachionus rugosa culture method of the present invention, cultivates Brachionus rugosa according to the following steps:
（1）培养设施：选择淡水来源方便、面积1000m2、深1.0m的老化的旧池塘作为培养池塘，在褶皱臂尾轮虫培养前用150kg/亩的生石灰对培养池塘进行消毒、暴晒一个星期后，用100目的筛绢过滤进水至培养池塘中水深0.9m，在培养池塘对角安装二台功率为0.75kv的水车式增氧机； (1) Cultivation facilities: choose an aged old pond with a convenient source of fresh water, an area of 1000m 2 , and a depth of 1.0m as the cultivation pond, and use 150kg/mu of quicklime to disinfect the cultivation pond and expose it to the sun for a week before cultivating Brachionus rugosa. Finally, use 100 mesh sieves to filter the incoming water to a water depth of 0.9m in the cultivation pond, and install two waterwheel type aerators with a power of 0.75kv at the diagonal of the cultivation pond;
（2）基础饵料：以小杂鱼为培养褶皱臂尾轮虫的基础饵料，小杂鱼用量为190kg/亩，将小杂鱼按20kg/袋，用80目的网袋盛装，然后扎紧网袋袋口，将装有小杂鱼的网袋均匀地吊挂于培养池塘的水中； (2) Basic bait: Use small trash fish as the basic bait for cultivating Brachionus rugosa, the amount of small trash fish is 190kg/mu, put the small trash fish in 20kg/bag, put them in 80-mesh mesh bags, and then tie the net tightly At the mouth of the bag, evenly hang the net bag with small miscellaneous fish in the water of the cultivation pond;
（3）日常管理：利用淡水调节培养池塘塘水比重，使培养池塘塘水比重维持在1.015；小杂鱼在培养池塘塘水中10天开始腐烂发酵，此时每天上午8时、下午6时开动增氧机，每次开动1.5小时，培养15天，当褶皱臂尾轮虫的密度达到250个/mL时，开始轮虫收集袋收集褶皱臂尾轮虫； (3) Daily management: use fresh water to adjust the specific gravity of the water in the cultivation pond, so that the specific gravity of the cultivation pond water is maintained at 1.015; small miscellaneous fish begin to rot and ferment in the water of the cultivation pond for 10 days, and start at 8:00 am and 6:00 pm every day Aerator, start 1.5 hours each time, cultivate 15 days, when the density of Brachionus rugosa reaches 250/mL, start the rotifer collection bag to collect Brachionus rugosa;
（4）轮虫收集： (4) Rotifer collection:
1）准备两个轮虫收集袋：用100目筛绢制作而成长5m、直径30cm且两端开口的圆形的100目筒形袋和用250目的筛绢制作而成长5.5m、直径50cm的250目筒形袋； 1) Prepare two rotifer collection bags: a circular 100-mesh cylindrical bag made of 100-mesh sieve silk with a length of 5m, a diameter of 30cm and openings at both ends, and a rotifer bag made of 250-mesh sieve with a length of 5.5m and a diameter of 50cm 250 mesh tubular bag;
2）轮虫收集方法：将250目筒形袋套在100目筒形袋的外面，将两个轮虫收集袋的一端开口分别扎紧，另一端开口均绑在用木桩固定在离培养池塘底部高50cm处的潜水泵的出水口； 2) The method of collecting rotifers: Put a 250-mesh cylindrical bag on the outside of a 100-mesh cylindrical bag, tie the openings at one end of the two rotifer collection bags tightly respectively, and tie the openings at the other end to a wooden stake and fix it in a separate culture area. The outlet of the submersible pump at a height of 50 cm from the bottom of the pond;
3）收集至250目筒形袋中褶皱臂尾轮虫的数量已较多，取下两个轮虫收集袋，从250目筒形袋中收取褶皱臂尾轮虫； 3) The number of Brachionus rugosa collected in the 250-mesh cylindrical bag is already large, remove the two rotifer collection bags, and collect the Brachionus rugosa from the 250-mesh cylindrical bag;
4）收集至观测到培养池塘中褶皱臂尾轮虫的繁育速度下降、褶皱臂尾轮虫密度已降低至110个/mL，表明池塘中的营养物质已满足不了褶皱臂尾轮虫生长的需要，因此追加小杂鱼的吊挂，追加量为45kg/亩。 4) From collection to observation, the breeding speed of Brachionus rugosa in the culture pond has decreased, and the density of Brachionus rugosa has dropped to 110/mL, indicating that the nutrients in the pond can no longer meet the growth needs of Brachionus rugosa , so the hanging of small miscellaneous fish is added, and the additional amount is 45kg/mu.
实施例4 采用本发明的褶皱臂尾轮虫培养方法，按照下列步骤培养褶皱臂尾轮虫： Embodiment 4 adopts Brachionus rugosa culture method of the present invention, cultivates Brachionus rugosa according to the following steps:
（1）培养设施：选择淡水来源方便、面积750m2、深1.05m的老化的旧池塘作为培养池塘，在褶皱臂尾轮虫培养前用4kg/亩的漂白精对培养池塘进行消毒、暴晒一个星期后，用100目的筛绢过滤进水至培养池塘中水深0.8m，在培养池塘对角安装二台功率为0.75kv的水车式增氧机； (1) Cultivation facilities: Choose an old aged pond with a convenient source of fresh water, an area of 750m 2 , and a depth of 1.05m as the cultivation pond. Before the cultivation of Brachionus rugosa, the cultivation pond is disinfected with 4kg/mu of bleach and exposed to the sun. After one week, filter the incoming water with 100 mesh sieves to the water depth of 0.8m in the cultivation pond, and install two waterwheel type aerators with a power of 0.75kv at the diagonal of the cultivation pond;
（2）基础饵料：以小杂鱼为培养褶皱臂尾轮虫的基础饵料，小杂鱼用量为180kg/亩，将小杂鱼按15kg/袋，用80目的网袋盛装，然后扎紧网袋袋口，将装有小杂鱼的网袋均匀地吊挂于培养池塘的水中； (2) Basic bait: use small trash fish as the basic bait for cultivating Brachionus plicaulata, the amount of small trash fish is 180kg/mu, put the small trash fish in 15kg/bag, put them in 80-mesh mesh bags, and then tie the net tightly At the mouth of the bag, evenly hang the net bag with small miscellaneous fish in the water of the cultivation pond;
（3）日常管理：利用淡水调节培养池塘塘水比重，使培养池塘塘水比重维持在1.010；小杂鱼在培养池塘塘水中12天开始腐烂发酵，此时每天上午8时、下午6时开动增氧机，每次开动1.0小时，培养18天，当褶皱臂尾轮虫的密度达到250个/mL时，开始轮虫收集袋收集褶皱臂尾轮虫； (3) Daily management: use fresh water to adjust the specific gravity of the water in the cultivation pond, so that the specific gravity of the cultivation pond water is maintained at 1.010; small miscellaneous fish begin to rot and ferment in the water of the cultivation pond for 12 days, and start at 8:00 am and 6:00 pm every day Aerator, start 1.0 hour each time, cultivate 18 days, when the density of Brachionus rugosa reaches 250/mL, start the rotifer collection bag to collect Brachionus rugosa;
（4）轮虫收集： (4) Rotifer collection:
1）准备两个轮虫收集袋：用100目筛绢制作而成长5m、直径30cm且两端开口的圆形的100目筒形袋和用250目的筛绢制作而成长5.5m、直径50cm的250目筒形袋； 1) Prepare two rotifer collection bags: a circular 100-mesh cylindrical bag made of 100-mesh sieve silk with a length of 5m, a diameter of 30cm and openings at both ends, and a rotifer bag made of 250-mesh sieve with a length of 5.5m and a diameter of 50cm 250 mesh tubular bag;
2）轮虫收集方法：将250目筒形袋套在100目筒形袋的外面，将两个轮虫收集袋的一端开口分别扎紧，另一端开口均绑在用木桩固定在离培养池塘底部高50cm处的潜水泵的出水口； 2) The method of collecting rotifers: Put a 250-mesh cylindrical bag on the outside of a 100-mesh cylindrical bag, tie the openings at one end of the two rotifer collection bags tightly respectively, and tie the openings at the other end to a wooden stake and fix it in a separate culture area. The outlet of the submersible pump at a height of 50 cm from the bottom of the pond;
3）收集至250目筒形袋中褶皱臂尾轮虫的数量已较多，取下两个轮虫收集袋，从250目筒形袋中收取褶皱臂尾轮虫； 3) The number of Brachionus rugosa collected in the 250-mesh cylindrical bag is already large, remove the two rotifer collection bags, and collect the Brachionus rugosa from the 250-mesh cylindrical bag;
4）收集至观测到培养池塘中褶皱臂尾轮虫的繁育速度下降、褶皱臂尾轮虫密度已降低至90个/mL，表明池塘中的营养物质已满足不了褶皱臂尾轮虫生长的需要，因此追加小杂鱼的吊挂，追加量为40kg/亩。 4) From collection to observation, the breeding speed of Brachionus rugosa in the culture pond has decreased, and the density of Brachionus rugosa has dropped to 90/mL, indicating that the nutrients in the pond can no longer meet the growth needs of Brachionus rugosa , so the hanging of small miscellaneous fish is added, and the additional amount is 40kg/mu.
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| CN105230577A (en) * | 2015-11-30 | 2016-01-13 | 江苏纳克生物工程有限公司 | Continuous culturing method for brachionus calyciflorus |
| CN105230577B (en) * | 2015-11-30 | 2018-03-30 | 江苏纳克生物工程有限公司 | The continuous cultural method of Brachionus calyciflorus |
| CN105393991A (en) * | 2015-12-09 | 2016-03-16 | 苏州大学 | Calyx Branchionus culturing method and detection method for toxicity of ammonia nitrogen to calyx Branchionus |
| CN105393991B (en) * | 2015-12-09 | 2018-08-21 | 苏州大学 | A kind of detection method of toxicity of ammonia nitrogen to Brachionus calyciflorus |
| CN106069895A (en) * | 2016-06-15 | 2016-11-09 | 安徽省皖江水产技术研究院有限责任公司 | The construction method of a kind of Heterologous Hybridization strain and offspring seed cultivation method thereof |
| CN108124796A (en) * | 2018-01-08 | 2018-06-08 | 广西壮族自治区海洋研究所 | A kind of method for cultivating wheel animalcule |
| CN110326562A (en) * | 2019-07-10 | 2019-10-15 | 盛世生态环境股份有限公司 | Large-scale branch pin class zooplankter high-density breeding method |
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