CN102511432A - Method for cultivating Brachionus plicatilis - Google Patents

Method for cultivating Brachionus plicatilis Download PDF

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Publication number
CN102511432A
CN102511432A CN2012100104618A CN201210010461A CN102511432A CN 102511432 A CN102511432 A CN 102511432A CN 2012100104618 A CN2012100104618 A CN 2012100104618A CN 201210010461 A CN201210010461 A CN 201210010461A CN 102511432 A CN102511432 A CN 102511432A
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brachionus plicatilis
pond
cultivating
water
brachionus
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CN102511432B (en
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罗杰
刘楚吾
曹伏君
杜涛
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Guangdong Ocean University
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Guangdong Ocean University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a method for cultivating Brachionus plicatilis, belonging to the technical field of aquaculture. The method for cultivating the Brachionus plicatilis by taking a waste or old pool as a cultivation pool and small trash fishes as basic bait materials comprises the following specific steps of: feeding water to the cultivation pool subjected to disinfection and exposure treatments, mounting an aerator, suspending the basic bait materials contained in a net bag according to a constant amount in water of the cultivation pool, cultivating for 13-18 days during daily management by combining with the aerator, beginning collection the Brachionus plicatilis by a Brachionus plicatilis collection bag when the density of the Brachionus plicatilis is up to 250 pieces/mL, and adding the suspension amount of the trash fishes according to a breeding speed and the density of the Brachionus plicatilis. The method for cultivating the Brachionus plicatilis, disclosed by the invention has the advantages of simpleness, convenience, large density of cultivated Brachionus plicatilis, comprehensive nutrition, long fertility in the water without frequently bait feeding, and capabilities of largely reducing production cost and improving economic benefit.

Description

A kind of cultural method of brachionus plicatilis
Technical field
The present invention relates to the cultural method of a kind of cultural method, particularly a kind of brachionus plicatilis of good biological feed in the marine economic animal seed rearing process, belong to technical field of aquaculture.
Background technology
Brachionus plicatilis (Brachionus plicatilis) belongs to Rotatoria, Monogonota, swimming suborder, arm tail Rotaliidae; It has wide gentle eurysalinity, strong to adaptive capacity to environment, growth and breeding speed fast, suitable size (100 μ m~300 μ m) and characteristics such as nutritious, become the good biological feed of important marine economic animal seed rearings such as fish, shrimp, crab and jellyfish.Generally less when the seed of seafood fish hatches, except that few species, their body is grown up between 2~7mm, and its bore is littler during initial feeding, and therefore, the open-mouthed bait nutritious, that size is agreeable to the taste of throwing something and feeding is the Technique in Fishes key of success of growing seedlings.Wheel animalcule is applied to the production of seafood fish seed and starts from late 1960s, the beginning of the seventies; The Japan scholar at first uses brachionus plicatilis and succeeds in the seed large-scale production of porgy (Pagrus major); Wheel animalcule just becomes the general food organisms that all seafood fish seeds produce afterwards; Up to now, with wheel animalcule as open-mouthed bait successfully carry out marine products economic fish that seed produces reached 60 surplus kind.
The wheel animalcule feeding habits are extensive, the subtitle substance in the water of mainly ingesting, and unit cell algae, bacterium, yeast, even organic debrises such as soya-bean milk, fermentation poultry dung slurry, straw pulp all can be used as it and cultivate bait.Traditional wheel animalcule is cultivated, and generally adopts unit cell algae, bacterium or yeast as its bait.Though bacteriums such as unit cell such as chlorella, flat algae algae and photosynthetic bacteria are cultivated the wheel animalcule comprehensive nutrition; Bait effect is good, still, owing to receive unit cell algae and photosynthetic bacteria to cultivate the restriction in place; Carrying out extensive wheel animalcule when cultivating, the amount of unit cell algae and photosynthetic bacteria is difficult to accomplish the capacity supply; Secondly because wheel animalcule is heavy, with unit cell algae and photosynthetic bacteria cultivation wheel animalcule, generally speaking wheel animalcule density be difficult to reach 200/more than the mL.Therefore; In the actual production, carry out large-scale high density wheel animalcule and cultivate, the bait that yeast commonly used is cultivated as wheel animalcule; But the unsaturated fatty acid that lacks fish growth with the wheel animalcule of Yeast Cultivation; Must strengthen through lipid nutritions such as the unit cell algae of short time or cuttlefish liver oils when wheel animalcule is thrown something and fed, and the yeast water of when indoor cement pit is cultivated, throwing something and feeding blames with control, it is high to cultivate cost.Therefore, adopt the method that facility is simple and crude, production cost is low to cultivate wheel animalcule, can realize the maximization of economic benefit.
Summary of the invention
The object of the invention is to provide a kind of cultural method of brachionus plicatilis, and this method is simple to operation, can realize the cultivation of large-scale high density brachionus plicatilis, and production cost is low, and economic benefit is high.
For addressing the above problem, the technical scheme that the present invention adopted is: a kind of cultural method of brachionus plicatilis comprises the steps:
(1) cultivates facility: convenient, the area 667.7~1000m of selection fresh water sources 2, dark 1.0~1.2m discarded or aging old pond as cultivating the pond; Before brachionus plicatilis is cultivated, the cultivation pond was carried out disinfection, is exposed to the sun after a week with the bleaching essence of the quicklime of 120-150kg/ mu or 4-5kg/ mu; To cultivating the depth of water 0.8~1.0m in the pond, cultivating installation two waterwheel aerators that power is 0.75kv in diagonal angle, pond with the water inlet of 100 purpose silk cover filterings;
(2) foundation bait: with the small fish is the foundation bait of cultivating brachionus plicatilis; The small fish consumption is 180~200kg/ mu, and small fish is pressed the 15-20kg/ bag, with 80 purpose mesh bag splendid attires; Tighten the mesh bag sack then, the mesh bag that small fish is housed is hung on equably in the water of cultivating the pond;
(3) daily management: utilize fresh water to regulate and cultivate the pool, pond water proportion, the cultivation pool, pond water proportion is maintained between 1.010~1.015; Small fish fermentation that putresces in 8-12 days in cultivating the pool, pond water, 8 o'clock every mornings of this moment, at 6 o'clock in afternoon are started aerator, start 0.5-1.5 hour at every turn, cultivate 13-18 days; When the density of brachionus plicatilis reaches 250/mL, begin to collect brachionus plicatilis with the wheel animalcule collecting bag; Be collected into observe cultivate brachionus plicatilis in the pond the speed of breeding descend, in time append when density obviously reduces and hang small fish, additional quantity is 40~50kg/ mu.
Wheel animalcule collecting bag in the said step (3) is for being made with 100 mesh sieve thin,tough silk and 100 order tubular bags of the circle of both ends open and be made and 250 order tubular bags of the circle of both ends open with 250 purpose bolting silks: the long 5m of 100 order tubular bags, diameter 30cm are used to filter out bigger object; The long 5.5m of 250 order tubular bags, diameter 50cm are used to collect wheel animalcule.
Method with wheel animalcule collecting bag collection brachionus plicatilis in the said step (3) is following: during collection; 250 order tubular bags are enclosed within the outside of 100 order tubular bags; One end opening of two wheel animalcule collecting bags is tightened respectively, and other end opening is all tied up the delivery port place at immersible pump.
Said immersible pump is fixed on from cultivating the high 50cm of pond bottom place with timber.
The present invention with respect to the beneficial effect of prior art is: the present invention be according to brachionus plicatilis life, ingest and reproductive characteristic, utilize discarded or the little old pond of value, be foundation bait with the small fish; Also supporting simultaneously oxygen increasing equipment carries out the cultivation of brachionus plicatilis, brachionus plicatilis cultural method according to the invention; Improved the cumbersome of bait of often to throw something and feed in the cultivation of wheel animalcule tradition; Simple to operation, the brachionus plicatilis density of being cultivated big (250/more than the mL), comprehensive nutrition need not carry out the hot housing seedling of just can directly throwing something and feeding with unit cell algae or other materials; And fertility is permanent in the water; Satisfy wheel animalcule and grow, breed required nutriment for a long time, thereby reduced production cost, remarkable in economical benefits improves.
Embodiment
Through embodiment the present invention is explained further details below, these embodiment only are used for explaining the present invention, do not limit the scope of the invention.
Embodiment 1Adopt brachionus plicatilis cultural method of the present invention, cultivate brachionus plicatilis according to the following step:
(1) cultivates facility: convenient, the area 667.7m of selection fresh water sources 2, dark 1.2m discarded old pond as the cultivation pond; Before brachionus plicatilis is cultivated, the cultivation pond was carried out disinfection, is exposed to the sun after a week with the quicklime of 150kg/ mu; To cultivating depth of water 0.85m in the pond, cultivating installation two waterwheel aerators that power is 0.75kv in diagonal angle, pond with the water inlet of 100 purpose silk cover filterings;
(2) foundation bait: with the small fish is the foundation bait of cultivating brachionus plicatilis; The small fish consumption is a 188kg/ mu, and small fish is pressed the 15kg/ bag, with 80 purpose mesh bag splendid attires; Tighten the mesh bag sack then, the mesh bag that small fish is housed is hung on equably in the water of cultivating the pond;
(3) daily management: utilize fresh water to regulate and cultivate the pool, pond water proportion, make the cultivation pool, pond water proportion maintain 1.010; Small fish fermentation that putresces in 8 days in cultivating the pool, pond water; 8 o'clock every mornings of this moment, at 6 o'clock in afternoon are started aerator, start 0.5 hour at every turn, cultivate 15 days; When the density of brachionus plicatilis reached 250/mL, beginning wheel animalcule collecting bag was collected brachionus plicatilis;
(4) wheel animalcule is collected:
1) prepare two wheel animalcule collecting bags: with 100 mesh sieve thin,tough silk be made long 5m, diameter 30cm and both ends open circle 100 order tubular bags and with the be made 250 order tubular bags of long 5.5m, diameter 50cm of 250 purpose bolting silks;
2) wheel animalcule collection method: 250 order tubular bags are enclosed within the outside of 100 order tubular bags, an end opening of two wheel animalcule collecting bags is tightened respectively, other end opening is all tied up at the delivery port that is fixed on timber from the immersible pump of cultivating the high 50cm of pond bottom place;
3) it is more to be collected into the quantity of brachionus plicatilis in the 250 order tubular bags, takes off two wheel animalcule collecting bags, from 250 order tubular bags, collects brachionus plicatilis;
4) be collected into the speed of the breeding decline, the brachionus plicatilis density that observe brachionus plicatilis in the cultivation pond and be reduced to 100/mL; Show that the nutriment in the pond has satisfied not the needs of brachionus plicatilis growth; Therefore append hanging of small fish, additional quantity is a 48kg/ mu.
Embodiment 2Adopt brachionus plicatilis cultural method of the present invention, cultivate brachionus plicatilis according to the following step:
(1) cultivates facility: convenient, the area 826m of selection fresh water sources 2, dark 1.1m discarded old pond as the cultivation pond; Before brachionus plicatilis is cultivated, the cultivation pond was carried out disinfection, is exposed to the sun after a week with the bleaching essence of 5kg/ mu; To cultivating depth of water 1.0m in the pond, cultivating installation two waterwheel aerators that power is 0.75kv in diagonal angle, pond with the water inlet of 100 purpose silk cover filterings;
(2) foundation bait: with the small fish is the foundation bait of cultivating brachionus plicatilis; The small fish consumption is a 200kg/ mu, and small fish is pressed the 17kg/ bag, with 80 purpose mesh bag splendid attires; Tighten the mesh bag sack then, the mesh bag that small fish is housed is hung on equably in the water of cultivating the pond;
(3) daily management: utilize fresh water to regulate and cultivate the pool, pond water proportion, make the cultivation pool, pond water proportion maintain 1.013; Small fish fermentation that putresces in 10 days in cultivating the pool, pond water; 8 o'clock every mornings of this moment, at 6 o'clock in afternoon are started aerator, start 1.2 hours at every turn, cultivate 13 days; When the density of brachionus plicatilis reached 250/mL, beginning wheel animalcule collecting bag was collected brachionus plicatilis;
(4) wheel animalcule is collected:
1) prepare two wheel animalcule collecting bags: with 100 mesh sieve thin,tough silk be made long 5m, diameter 30cm and both ends open circle 100 order tubular bags and with the be made 250 order tubular bags of long 5.5m, diameter 50cm of 250 purpose bolting silks;
2) wheel animalcule collection method: 250 order tubular bags are enclosed within the outside of 100 order tubular bags, an end opening of two wheel animalcule collecting bags is tightened respectively, other end opening is all tied up at the delivery port that is fixed on timber from the immersible pump of cultivating the high 50cm of pond bottom place;
3) it is more to be collected into the quantity of brachionus plicatilis in the 250 order tubular bags, takes off two wheel animalcule collecting bags, from 250 order tubular bags, collects brachionus plicatilis;
4) be collected into the speed of the breeding decline, the brachionus plicatilis density that observe brachionus plicatilis in the cultivation pond and be reduced to 80/mL; Show that the nutriment in the pond has satisfied not the needs of brachionus plicatilis growth; Therefore append hanging of small fish, additional quantity is a 50kg/ mu.
Embodiment 3Adopt brachionus plicatilis cultural method of the present invention, cultivate brachionus plicatilis according to the following step:
(1) cultivates facility: convenient, the area 1000m of selection fresh water sources 2, dark 1.0m aging old pond as cultivating the pond; Before brachionus plicatilis is cultivated, the cultivation pond was carried out disinfection, is exposed to the sun after a week with the quicklime of 150kg/ mu; To cultivating depth of water 0.9m in the pond, cultivating installation two waterwheel aerators that power is 0.75kv in diagonal angle, pond with the water inlet of 100 purpose silk cover filterings;
(2) foundation bait: with the small fish is the foundation bait of cultivating brachionus plicatilis; The small fish consumption is a 190kg/ mu, and small fish is pressed the 20kg/ bag, with 80 purpose mesh bag splendid attires; Tighten the mesh bag sack then, the mesh bag that small fish is housed is hung on equably in the water of cultivating the pond;
(3) daily management: utilize fresh water to regulate and cultivate the pool, pond water proportion, make the cultivation pool, pond water proportion maintain 1.015; Small fish fermentation that putresces in 10 days in cultivating the pool, pond water; 8 o'clock every mornings of this moment, at 6 o'clock in afternoon are started aerator, start 1.5 hours at every turn, cultivate 15 days; When the density of brachionus plicatilis reached 250/mL, beginning wheel animalcule collecting bag was collected brachionus plicatilis;
(4) wheel animalcule is collected:
1) prepare two wheel animalcule collecting bags: with 100 mesh sieve thin,tough silk be made long 5m, diameter 30cm and both ends open circle 100 order tubular bags and with the be made 250 order tubular bags of long 5.5m, diameter 50cm of 250 purpose bolting silks;
2) wheel animalcule collection method: 250 order tubular bags are enclosed within the outside of 100 order tubular bags, an end opening of two wheel animalcule collecting bags is tightened respectively, other end opening is all tied up at the delivery port that is fixed on timber from the immersible pump of cultivating the high 50cm of pond bottom place;
3) it is more to be collected into the quantity of brachionus plicatilis in the 250 order tubular bags, takes off two wheel animalcule collecting bags, from 250 order tubular bags, collects brachionus plicatilis;
4) be collected into the speed of the breeding decline, the brachionus plicatilis density that observe brachionus plicatilis in the cultivation pond and be reduced to 110/mL; Show that the nutriment in the pond has satisfied not the needs of brachionus plicatilis growth; Therefore append hanging of small fish, additional quantity is a 45kg/ mu.
Embodiment 4Adopt brachionus plicatilis cultural method of the present invention, cultivate brachionus plicatilis according to the following step:
(1) cultivates facility: convenient, the area 750m of selection fresh water sources 2, dark 1.05m aging old pond as cultivating the pond; Before brachionus plicatilis is cultivated, the cultivation pond was carried out disinfection, is exposed to the sun after a week with the bleaching essence of 4kg/ mu; To cultivating depth of water 0.8m in the pond, cultivating installation two waterwheel aerators that power is 0.75kv in diagonal angle, pond with the water inlet of 100 purpose silk cover filterings;
(2) foundation bait: with the small fish is the foundation bait of cultivating brachionus plicatilis; The small fish consumption is a 180kg/ mu, and small fish is pressed the 15kg/ bag, with 80 purpose mesh bag splendid attires; Tighten the mesh bag sack then, the mesh bag that small fish is housed is hung on equably in the water of cultivating the pond;
(3) daily management: utilize fresh water to regulate and cultivate the pool, pond water proportion, make the cultivation pool, pond water proportion maintain 1.010; Small fish fermentation that putresces in 12 days in cultivating the pool, pond water; 8 o'clock every mornings of this moment, at 6 o'clock in afternoon are started aerator, start 1.0 hours at every turn, cultivate 18 days; When the density of brachionus plicatilis reached 250/mL, beginning wheel animalcule collecting bag was collected brachionus plicatilis;
(4) wheel animalcule is collected:
1) prepare two wheel animalcule collecting bags: with 100 mesh sieve thin,tough silk be made long 5m, diameter 30cm and both ends open circle 100 order tubular bags and with the be made 250 order tubular bags of long 5.5m, diameter 50cm of 250 purpose bolting silks;
2) wheel animalcule collection method: 250 order tubular bags are enclosed within the outside of 100 order tubular bags, an end opening of two wheel animalcule collecting bags is tightened respectively, other end opening is all tied up at the delivery port that is fixed on timber from the immersible pump of cultivating the high 50cm of pond bottom place;
3) it is more to be collected into the quantity of brachionus plicatilis in the 250 order tubular bags, takes off two wheel animalcule collecting bags, from 250 order tubular bags, collects brachionus plicatilis;
4) be collected into the speed of the breeding decline, the brachionus plicatilis density that observe brachionus plicatilis in the cultivation pond and be reduced to 90/mL; Show that the nutriment in the pond has satisfied not the needs of brachionus plicatilis growth; Therefore append hanging of small fish, additional quantity is a 40kg/ mu.

Claims (4)

1. the cultural method of a brachionus plicatilis is characterized in that: comprise the steps:
(1) cultivates facility: convenient, the area 667.7~1000m of selection fresh water sources 2, dark 1.0~1.2m discarded or aging old pond as cultivating the pond; Before brachionus plicatilis is cultivated, the cultivation pond was carried out disinfection, is exposed to the sun after a week with the bleaching essence of the quicklime of 120-150kg/ mu or 4-5kg/ mu; To cultivating the depth of water 0.8~1.0m in the pond, cultivating installation two waterwheel aerators that power is 0.75kv in diagonal angle, pond with the water inlet of 100 purpose silk cover filterings;
(2) foundation bait: with the small fish is the foundation bait of cultivating brachionus plicatilis; The small fish consumption is 180~200kg/ mu, and small fish is pressed the 15-20kg/ bag, with 80 purpose mesh bag splendid attires; Tighten the mesh bag sack then, the mesh bag that small fish is housed is hung on equably in the water of cultivating the pond;
(3) daily management: utilize fresh water to regulate and cultivate the pool, pond water proportion, the cultivation pool, pond water proportion is maintained between 1.010~1.015; Small fish fermentation that putresces in 8-12 days in cultivating the pool, pond water, 8 o'clock every mornings of this moment, at 6 o'clock in afternoon are started aerator, start 0.5-1.5 hour at every turn, cultivate 13-18 days; When the density of brachionus plicatilis reaches 250/mL, begin to collect brachionus plicatilis with the wheel animalcule collecting bag; Be collected into observe cultivate brachionus plicatilis in the pond the speed of breeding descend, in time append when density obviously reduces and hang small fish, additional quantity is 40~50kg/ mu.
2. according to the cultural method of the described brachionus plicatilis of claim 1; It is characterized in that: the wheel animalcule collecting bag in the said step (3) is for being made with 100 mesh sieve thin,tough silk and 100 order tubular bags of the circle of both ends open and be made and 250 order tubular bags of the circle of both ends open with 250 purpose bolting silks: the long 5m of 100 order tubular bags, diameter 30cm are used to filter out bigger object; The long 5.5m of 250 order tubular bags, diameter 50cm are used to collect wheel animalcule.
3. according to the cultural method of the described brachionus plicatilis of claim 1; It is characterized in that: the method with wheel animalcule collecting bag collection brachionus plicatilis in the said step (3) is following: during collection; 250 order tubular bags are enclosed within the outside of 100 order tubular bags; One end opening of two wheel animalcule collecting bags is tightened respectively, and other end opening is all tied up the delivery port place at immersible pump.
4. according to the described method of collecting brachionus plicatilis with the wheel animalcule collecting bag of claim 3, it is characterized in that: said immersible pump is fixed on from cultivating the high 50cm of pond bottom place with timber.
CN2012100104618A 2012-01-13 2012-01-13 Method for cultivating Brachionus plicatilis Expired - Fee Related CN102511432B (en)

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CN103141441A (en) * 2012-10-23 2013-06-12 中国水产科学研究院东海水产研究所 Method for cultivating rotifers by using dried yeast and Antarctic krill meal
CN103858815A (en) * 2012-12-18 2014-06-18 上海市水产研究所 Method for ecologically cultivating rotifers in summer eastern puffer fish fingerling culture pond
CN104067959A (en) * 2013-09-12 2014-10-01 江苏省淡水水产研究所 Method for improving large loach fry breeding survival rate
CN104082248A (en) * 2014-06-23 2014-10-08 浙江省淡水水产研究所 Method for small-scale and simple culturing of freshwater rotifers
CN105230577A (en) * 2015-11-30 2016-01-13 江苏纳克生物工程有限公司 Continuous culturing method for brachionus calyciflorus
CN105393991A (en) * 2015-12-09 2016-03-16 苏州大学 Calyx Branchionus culturing method and detection method for toxicity of ammonia nitrogen to calyx Branchionus
CN106069895A (en) * 2016-06-15 2016-11-09 安徽省皖江水产技术研究院有限责任公司 The construction method of a kind of Heterologous Hybridization strain and offspring seed cultivation method thereof
CN108124796A (en) * 2018-01-08 2018-06-08 广西壮族自治区海洋研究所 A kind of method for cultivating wheel animalcule
CN110326562A (en) * 2019-07-10 2019-10-15 盛世生态环境股份有限公司 Large-scale branch pin class zooplankter high-density breeding method

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CN103141441B (en) * 2012-10-23 2014-06-04 中国水产科学研究院东海水产研究所 Method for cultivating rotifers by using dried yeast and Antarctic krill meal
CN103141441A (en) * 2012-10-23 2013-06-12 中国水产科学研究院东海水产研究所 Method for cultivating rotifers by using dried yeast and Antarctic krill meal
CN103858815A (en) * 2012-12-18 2014-06-18 上海市水产研究所 Method for ecologically cultivating rotifers in summer eastern puffer fish fingerling culture pond
CN103858815B (en) * 2012-12-18 2015-09-09 上海市水产研究所 A kind of interior ecological method of cultivating wheel animalcule of Fugu fingerling culturing pool in summer
CN104067959A (en) * 2013-09-12 2014-10-01 江苏省淡水水产研究所 Method for improving large loach fry breeding survival rate
CN104082248B (en) * 2014-06-23 2016-01-20 浙江省淡水水产研究所 The method of the simple and easy cultivation freshwater rotifer of a kind of small-scale
CN104082248A (en) * 2014-06-23 2014-10-08 浙江省淡水水产研究所 Method for small-scale and simple culturing of freshwater rotifers
CN105230577A (en) * 2015-11-30 2016-01-13 江苏纳克生物工程有限公司 Continuous culturing method for brachionus calyciflorus
CN105230577B (en) * 2015-11-30 2018-03-30 江苏纳克生物工程有限公司 The continuous cultural method of Brachionus calyciflorus
CN105393991A (en) * 2015-12-09 2016-03-16 苏州大学 Calyx Branchionus culturing method and detection method for toxicity of ammonia nitrogen to calyx Branchionus
CN105393991B (en) * 2015-12-09 2018-08-21 苏州大学 A kind of detection method of toxicity of ammonia nitrogen to Brachionus calyciflorus
CN106069895A (en) * 2016-06-15 2016-11-09 安徽省皖江水产技术研究院有限责任公司 The construction method of a kind of Heterologous Hybridization strain and offspring seed cultivation method thereof
CN108124796A (en) * 2018-01-08 2018-06-08 广西壮族自治区海洋研究所 A kind of method for cultivating wheel animalcule
CN110326562A (en) * 2019-07-10 2019-10-15 盛世生态环境股份有限公司 Large-scale branch pin class zooplankter high-density breeding method

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