JP2003259760A - Taurine-enriched rotifers - Google Patents
Taurine-enriched rotifersInfo
- Publication number
- JP2003259760A JP2003259760A JP2002065682A JP2002065682A JP2003259760A JP 2003259760 A JP2003259760 A JP 2003259760A JP 2002065682 A JP2002065682 A JP 2002065682A JP 2002065682 A JP2002065682 A JP 2002065682A JP 2003259760 A JP2003259760 A JP 2003259760A
- Authority
- JP
- Japan
- Prior art keywords
- taurine
- rotifer
- enriched
- vitamin
- content
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 title claims abstract description 252
- 241000700141 Rotifera Species 0.000 title claims abstract description 170
- 229960003080 taurine Drugs 0.000 title claims abstract description 125
- 241000251468 Actinopterygii Species 0.000 claims abstract description 28
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims abstract description 28
- 229930003231 vitamin Natural products 0.000 claims abstract description 24
- 235000013343 vitamin Nutrition 0.000 claims abstract description 24
- 239000011782 vitamin Substances 0.000 claims abstract description 24
- 229940088594 vitamin Drugs 0.000 claims abstract description 24
- 238000004519 manufacturing process Methods 0.000 claims abstract description 19
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 16
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 14
- 241000195493 Cryptophyta Species 0.000 claims abstract description 12
- 239000000843 powder Substances 0.000 claims abstract description 12
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 34
- 238000009395 breeding Methods 0.000 claims description 23
- 230000001488 breeding effect Effects 0.000 claims description 23
- 235000014102 seafood Nutrition 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 17
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 16
- 229930003268 Vitamin C Natural products 0.000 claims description 16
- 235000019154 vitamin C Nutrition 0.000 claims description 16
- 239000011718 vitamin C Substances 0.000 claims description 16
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims description 15
- 238000009372 pisciculture Methods 0.000 claims description 2
- 230000037396 body weight Effects 0.000 claims 1
- 235000019688 fish Nutrition 0.000 abstract description 26
- 238000012258 culturing Methods 0.000 abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 12
- 235000015170 shellfish Nutrition 0.000 abstract description 9
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 241001282110 Pagrus major Species 0.000 description 10
- 241000269908 Platichthys flesus Species 0.000 description 9
- 235000016709 nutrition Nutrition 0.000 description 7
- 230000000366 juvenile effect Effects 0.000 description 6
- 239000013535 sea water Substances 0.000 description 6
- 230000004083 survival effect Effects 0.000 description 6
- 241000238366 Cephalopoda Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000003623 enhancer Substances 0.000 description 4
- 239000013505 freshwater Substances 0.000 description 4
- 230000012447 hatching Effects 0.000 description 4
- 230000035764 nutrition Effects 0.000 description 4
- 230000036186 satiety Effects 0.000 description 4
- 235000019627 satiety Nutrition 0.000 description 4
- 241000238413 Octopus Species 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000000384 rearing effect Effects 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- 241000555825 Clupeidae Species 0.000 description 2
- 101000652359 Homo sapiens Spermatogenesis-associated protein 2 Proteins 0.000 description 2
- 101000642464 Homo sapiens Spermatogenesis-associated protein 2-like protein Proteins 0.000 description 2
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 2
- 102100030254 Spermatogenesis-associated protein 2 Human genes 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 235000008429 bread Nutrition 0.000 description 2
- RGJHWLDSRIHFKY-FWCDDDAWSA-L calcium;(2r)-2-[(1s)-1,2-dihydroxyethyl]-4-hydroxy-5-oxo-2h-furan-3-olate;dihydrate Chemical compound O.O.[Ca+2].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] RGJHWLDSRIHFKY-FWCDDDAWSA-L 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 235000019512 sardine Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- UIERGBJEBXXIGO-UHFFFAOYSA-N thiamine mononitrate Chemical compound [O-][N+]([O-])=O.CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N UIERGBJEBXXIGO-UHFFFAOYSA-N 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000700106 Brachionus Species 0.000 description 1
- 241000700104 Brachionus plicatilis Species 0.000 description 1
- 241001247197 Cephalocarida Species 0.000 description 1
- 241000239250 Copepoda Species 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000257465 Echinoidea Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000237852 Mollusca Species 0.000 description 1
- 241000237503 Pectinidae Species 0.000 description 1
- 241000490567 Pinctada Species 0.000 description 1
- 241001600434 Plectroglyphidodon lacrymatus Species 0.000 description 1
- 241000269978 Pleuronectiformes Species 0.000 description 1
- 241000269851 Sarda sarda Species 0.000 description 1
- 241000276699 Seriola Species 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 238000001212 derivatisation Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 description 1
- 229960005135 eicosapentaenoic acid Drugs 0.000 description 1
- 235000020673 eicosapentaenoic acid Nutrition 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 229940067631 phospholipid Drugs 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 235000020637 scallop Nutrition 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 229960000344 thiamine hydrochloride Drugs 0.000 description 1
- 235000019190 thiamine hydrochloride Nutrition 0.000 description 1
- 239000011747 thiamine hydrochloride Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
Landscapes
- Feed For Specific Animals (AREA)
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明はタウリン強化ワム
シ、その生産方法および該タウリン強化ワムシを用いて
魚介類を飼育する方法に関する。本発明のタウリン強化
ワムシを用いて魚介類、特に仔稚魚を飼育すると、斃死
率の大幅な低減、活力の増加、生育促進を図りながら、
魚介類を健全に生産性良く飼育することができる。TECHNICAL FIELD The present invention relates to a taurine-enriched rotifer, a method for producing the same, and a method for breeding fish and shellfish using the taurine-enriched rotifer. Raising seafood, especially larvae, using the taurine-enriched rotifer of the present invention, while significantly reducing the mortality rate, increasing vitality, and promoting growth,
The seafood can be raised soundly and productively.
【０００２】[0002]
【従来の技術】海産魚類の種苗生産の餌料としては、生
物餌料であるワムシ、アルテミア、コペポーダなどが従
来から慣用されている。近年、ワムシに代わる種々の種
苗生産用飼料が開発、販売されているが、ワムシの全量
を代替可能な実用性のある飼料は未だ開発されておら
ず、そのため海産魚類の種苗生産は、ワムシなどの生物
餌料に頼らざるを得ないのが現状である。しかしなが
ら、ワムシなどの生物餌料も、魚類、特に仔稚魚に対す
る栄養面からみると十分なものではなく、飼育中に、魚
類の斃死、活力低下、生育不良などが生じ易いという問
題があった。2. Description of the Related Art As feed for producing seedlings of marine fish, rotifer, artemia, copepoda, etc., which are biological feeds, have been conventionally used. In recent years, various feeds for producing seedlings have been developed and sold in place of rotifers, but practical feeds that can replace the whole amount of rotifers have not yet been developed. The present situation is that we have no choice but to rely on the biological feed of the above. However, biological feeds such as rotifers are not sufficient from the nutritional point of view of fishes, particularly larvae, and there is a problem that death of fishes, reduction of vitality, poor growth and the like are likely to occur during breeding.
【０００３】海産魚類の種苗生産に用いられるワムシの
生産方法としては、クロレラ、藻類、酵母、魚卵粉末な
どの１種または２種以上を用いてワムシを培養する方法
が一般に採用されている。酵母や淡水クロレラを使用し
てワムシを培養する場合は、ワムシの大量培養が可能で
あるというメリットはあるが、それによって生産された
ワムシは、栄養面で欠けており、種苗生産に使用すると
仔稚魚の斃死、活力低下、生育不良などを招き易いとい
う欠点がある。一方、海産クロレラを用いて培養したワ
ムシは、酵母や淡水クロレラを使用して培養したワムシ
に比べて、ワムシ体内中でのω３ＨＵＦＡなどの栄養成
分の蓄積量が多くなり、そのためそれにより得られたワ
ムシを用いて海産魚類の種苗生産を行うと、酵母培養ワ
ムシや淡水クロレラ培養ワムシを使用した場合に比べ
て、仔稚魚の斃死、活力低下、生育不良などが生じにく
いという長所があるが、その効果は未だ十分に満足のゆ
くものではない。しかも、海産クロレラの培養には多大
な培養設備が必要であるという欠点がある。As a method of producing rotifers used for producing seedlings of marine fish, a method of cultivating rotifers using one or more of chlorella, algae, yeast, fish egg powder and the like is generally adopted. When cultivating rotifers using yeast or freshwater chlorella, there is an advantage that large-scale cultivation of rotifers is possible, but the rotifer produced by this is lacking in nutritional aspects, and when used for seedling production, it produces pups. It has the drawback that it is prone to death of larvae, loss of vitality, and poor growth. On the other hand, rotifers cultivated using marine chlorella have a greater amount of nutrients such as ω3HUFA accumulated in the rotifer body than rotifers cultivated using yeast or freshwater chlorella, and are thus obtained. Producing seedlings of marine fish using rotifers has the advantage that mortality of larvae, loss of vitality, poor growth, etc. are less likely to occur than when using yeast-cultured rotifers or freshwater chlorella-cultured rotifers. The effect is not yet fully satisfactory. Moreover, there is a drawback that a large amount of culture equipment is required for culturing marine chlorella.
【０００４】かかる点から、エイコサペンタエン酸（Ｅ
ＰＡ）やドコサコヘキサエン酸などのω３高度不飽和酸
（ω３ＨＵＦＡ）、リン脂質、ビタミンＡやビタミンＤ
などの脂溶性ビタミンなどの栄養成分を添加した強化剤
（餌料）を用いてワムシを培養し、ワムシ体内でのそれ
らの栄養成分の含有量を増強させ、それにより得られた
ワムシを用いて仔稚魚を飼育することが行われている。
しかしながら、その効果は未だ十分ではない。そのた
め、海産魚類の種苗生産時において、仔稚魚の斃死、活
力低下、生育不良などを従来よりも一層抑制して、仔稚
魚を健全に且つ生産性良く生育させることのできるワム
シが求められていた。From this point of view, eicosapentaenoic acid (E
PA) and ω3 highly unsaturated acids such as docosacohexaenoic acid (ω3HUFA), phospholipids, vitamin A and vitamin D
The rotifer is cultivated by using a fortifier (feeding material) to which nutrient components such as fat-soluble vitamins are added to increase the content of those nutrient components in the rotifer body, and the rotifer thus obtained is used. Raising fry is performed.
However, the effect is not yet sufficient. Therefore, during the production of seedlings of marine fish, there has been a demand for a rotifer that can suppress mortality, decrease in vitality, poor growth, etc. of larvae and larvae and grow larvae soundly and with good productivity. .
【０００５】[0005]
【発明が解決しようとする課題】本発明の目的は、魚介
類の飼育、特に海産魚類の種苗生産に用いたときに、魚
介類、特に仔稚魚の斃死、活力低下、生育不良などをよ
り効果的に防いで、魚介類、特に仔稚魚を健全に且つ生
産性よく生育させることのできるワムシを提供すること
である。さらに、本発明の目的は、魚介類を健全に且つ
生産性よく生育させることのできるワムシの生産方法を
提供することである。そして、本発明の目的は、前記し
たワムシを使用して魚介類を飼育する方法を提供するこ
とである。DISCLOSURE OF THE INVENTION The object of the present invention is to improve the effects of mortality, loss of vitality and poor growth of seafood, especially larvae, when used for breeding seafood, especially for producing seedlings of marine fish. The present invention aims to provide a rotifer capable of growing seafood, particularly larvae, in a healthy and productive manner by preventing the rotifer. Further, an object of the present invention is to provide a method for producing rotifer which enables healthy growth of fish and shellfish with good productivity. And an object of the present invention is to provide a method for breeding seafood using the above-mentioned rotifer.
【０００６】[0006]
【課題を解決するための手段】上記の目的を達成すべく
本発明者らは研究を重ねてきた。その結果、タウリンを
ワムシの体内に蓄積させてタウリン強化ワムシを生産
し、そのタウリン強化ワムシを用いて魚介類の仔稚魚を
飼育すると、斃死率が低下すると共に活力の増加および
生育促進を図ることができ、魚介類の飼育、特に種苗生
産が極めて円滑に生産性よく行えることを見出した。さ
らに、本発明者らは、そのようなタウリン強化ワムシ
は、タウリンの添加下に、クロレラ、藻類、酵母、魚卵
粉末などの少なくとも１種を餌料として用いて培養する
ことによって生産でき、そのようにして生産されたワム
シはタウリン以外の点でも栄養的に優れていて、良好な
飼育成績が得られることを見出した。また、本発明者ら
は、タウリン強化ワムシの生産時に、餌料中にタウリン
と共に更に水溶性ビタミン、特にビタミンＢ1および／
またはビタミンＣを添加しておくと、タウリンと共にそ
れらのビタミンもワムシの体内に取り込まれて、タウリ
ンおよび水溶性ビタミンの増強された、魚介類に対する
栄養面で一層優れたタウリン強化ワムシが得られること
を見出し、それらの知見に基づいて本発明を完成した。Means for Solving the Problems The present inventors have conducted extensive research in order to achieve the above object. As a result, taurine is accumulated in the body of the rotifer to produce a taurine-enriched rotifer, and if taurine-enriched rotifer is used to raise larvae of seafood, the mortality rate will be reduced and the vitality will be increased and the growth promotion will be promoted. It was found that the breeding of seafood, especially the production of seeds and seedlings can be carried out very smoothly and with good productivity. Furthermore, the present inventors can produce such a taurine-enriched rotifer by culturing with the addition of taurine using at least one of chlorella, algae, yeast, fish egg powder, etc. as a feed, and It was found that the rotifers produced in this manner were nutritionally excellent in terms other than taurine and that good breeding results were obtained. In addition, the present inventors also confirmed that during the production of taurine-fortified rotifer, water-soluble vitamins, particularly vitamin B 1 and /
Alternatively, if vitamin C is added, those vitamins are taken into the body of the rotifer along with taurine, and taurine-enriched rotifer with enhanced taurine and water-soluble vitamins, which is more excellent in nutrition for seafood, can be obtained. The present invention has been completed based on these findings.
【０００７】すなわち、本発明は、
（１） 体内でのタウリン含量が増強されていることを
特徴とするタウリン強化ワムシである。That is, the present invention is (1) a taurine-enriched rotifer characterized by having an increased taurine content in the body.
【０００８】そして、本発明は、
（２） タウリン含量がワムシの体重に対して、０．０
１質量％以上である前記した（１）のタウリン強化ワム
シ；
（３） さらに、体内での水溶性ビタミンの含量が増強
されている前記した（１）または（２）のタウリン強化
ワムシ；
（４） 水溶性ビタミンが、ビタミンＢ1および／また
はビタミンＣである前記した（３）のタウリン強化ワム
シ；
（５） タウリンの添加下、またはタウリンと水溶性ビ
タミンの添加下に、クロレラ、藻類、酵母および魚卵粉
末のうちの少なくとも１種を用いて培養された前記した
（１）〜（４）のタウリン強化ワムシ；および、
（６） 養魚用餌料である前記した（１）〜（５）のい
ずれかのタウリン強化ワムシ；である。(2) The taurine content of the present invention is 0.0 relative to the weight of the rotifer.
(1) Taurine-enriched rotifer of 1% by mass or more; (3) Further, the taurine-enriched rotifer of (1) or (2) above, wherein the content of water-soluble vitamins in the body is enhanced; ) The water-soluble vitamin is vitamin B 1 and / or vitamin C, the above-mentioned (3) taurine-enriched rotifer; (5) under the addition of taurine or taurine and a water-soluble vitamin, chlorella, algae, yeast. And the taurine-enriched rotifer of (1) to (4), which has been cultivated using at least one of the above, and (6) the above-mentioned (1) to (5) which is a feed for fish farming. Any taurine-enriched rotifer;
【０００９】さらに、本発明は、
（７） タウリンの添加下またはタウリンと水溶性ビタ
ミンの添加下に、クロレラ、藻類、酵母および魚卵粉末
のうちの少なくとも１種を用いてワムシを培養すること
を特徴とする、体内でのタウリン含量、またはタウリン
含量と水溶性ビタミン含量の増強されたタウリン強化ワ
ムシの生産方法である。そして、本発明は、
（８） 水溶性ビタミンが、ビタミンＢ1および／また
はビタミンＣである前記した（７）の生産方法である。The present invention further provides (7) culturing a rotifer using at least one of chlorella, algae, yeast, and fish egg powder under the addition of taurine or taurine and a water-soluble vitamin. A method for producing a taurine-enriched rotifer having an increased taurine content in the body, or an increased taurine content and a water-soluble vitamin content. Then, the present invention is (8) the method for producing (7), wherein the water-soluble vitamin is vitamin B 1 and / or vitamin C.
【００１０】さらに、本発明は、
（９） 前記した（１）〜（６）のいずれかのタウリン
強化ワムシを用いて魚介類を飼育する方法である。Furthermore, the present invention is (9) a method for breeding seafood using the taurine-enriched rotifer of any one of (1) to (6) above.
【００１１】[0011]
【発明の実施の形態】以下に本発明について詳細に説明
する。本発明のタウリン強化ワムシのベースとなるワム
シは、魚介類の飼育時に生物餌料として用いられている
ワムシであればいずれでもよく、例えば、海産シオミズ
ツボワムシであるＳ型ワムシ（Brachionus rotundiform
is）およびＬ型ワムシ（Brachionus plicatilis）を挙
げることができる。BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below. The rotifer serving as the base of the taurine-enriched rotifer of the present invention may be any rotifer that is used as a biological feed during the breeding of seafood, for example, an S-type rotifer (Brachionus rotundiform) that is a marine sea urchin rotifer.
is) and L-type rotifer (Brachionus plicatilis).
【００１２】タウリンは、アミノエタンスルホン酸の別
称であり、牛の胆汁、イカ、タコ、貝類などの軟体動物
の肉エキス中に多量に存在し、その体内含量は、例えば
イカでは０．３５質量％程度、タコでは０．５質量％程
度、アコヤ貝では０．８質量％程度、また一般の魚類で
はそれよりも少なく、例えばイワシでは０．０２質量％
程度、カツオでは０．０８質量％程度と言われている。
これに対して、ワムシの体内にはタウリンは元々殆ど含
まれていなか、または含まれていてもその含有量は極め
て微量である。Taurine is another name for aminoethanesulfonic acid and is present in large amounts in bovine bile, meat extract of molluscs such as squid, octopus and shellfish, and its content in the body is, for example, 0.35 mass in squid. %, About 0.5% by mass for octopus, about 0.8% by mass for pearl oysters, and less than that for general fish, for example 0.02% by mass for sardines.
It is said that bonito is about 0.08% by mass.
In contrast, the body of rotifer originally contains almost no taurine, or contains taurine in an extremely small amount.
【００１３】本発明のタウリン強化ワムシは、元々タウ
リンを殆ど含有していないワムシの体内中に、タウリン
を外部から取り込ませて体内に蓄積させて、体内でのタ
ウリン含量を増強させたワムシである。本発明で用いる
タウリンは、天然物に由来するものであっても、合成し
たものであっても、または両者の併用であってもよく、
そのうちでもホタテやイワシなどの水産物に由来するタ
ウリンが好ましく用いられる。The taurine-enriched rotifer of the present invention is a rotifer that has been incorporated with taurine from the outside into the body of a rotifer that originally contains little taurine and accumulates it in the body to enhance the taurine content in the body. . Taurine used in the present invention may be derived from a natural product, may be synthetic, or may be a combination of both,
Among them, taurine derived from marine products such as scallops and sardines is preferably used.
【００１４】本発明のタウリン強化ワムシにおけるワム
シ体内でのタウリン含量は、ワムシを給餌する魚介類の
種類、全長、日令などに応じて異なり得るが、一般的に
はワムシの体重（生体質量）に基づいて、０．０１質量
％以上（乾燥したワムシの重量に対して０．１質量％以
上）であることが好ましく、０．０３質量％以上（乾燥
したワムシの重量に対して０．４質量％以上）であるこ
とがより好ましく、０．０５質量％以上（乾燥したワム
シの重量に対して０．６質量％以上）であることがより
好ましい。ワムシの体内でのタウリン含量が０．０１質
量％未満であると、そのようなワムシを生物餌料（生
餌）として用いて種苗生産を行ったときに、仔稚魚の斃
死、活力低下、生育不良などの効果的な抑制が困難にな
る。タウリン強化ワムシにおける体内でのタウリン含量
の上限値は特に制限されないが、タウリン強化ワムシの
生産（増殖）の容易性、コストなどの点から、ワムシの
体重に基づいて０．３質量％以下であることが好まし
い。The taurine content in the rotifer in the taurine-enriched rotifer of the present invention may vary depending on the type of fish and shellfish feeding the rotifer, the total length, the age, etc., but generally the rotifer's weight (biomass) It is preferably 0.01% by mass or more (0.1% by mass or more based on the weight of the dried rotifer), and 0.03% by mass or more (0.4% based on the weight of the dried rotifer). Mass% or more), and more preferably 0.05 mass% or more (0.6 mass% or more with respect to the weight of dried rotifer). When the taurine content in the body of rotifer is less than 0.01% by mass, when such a rotifer is used as a biological bait (live bait) for seedling production, mortality of larvae, decline in vitality, and poor growth It becomes difficult to effectively control such as. Although the upper limit of the taurine content in the body of the taurine-enriched rotifer is not particularly limited, it is 0.3% by mass or less based on the weight of the rotifer from the viewpoint of the ease of production (propagation) of the taurine-enriched rotifer and the cost. It is preferable.
【００１５】本発明のタウリン強化ワムシの生産方法は
特に制限されず、ワムシの体内にタウリンを蓄積させて
タウリン含量を増強し得る生産方法であればいずれの方
法も採用できる。そのうちでも、本発明のタウリン強化
ワムシは、タウリンの添加下に、クロレラ、藻類、酵
母、魚卵粉末などの少なくとも１種を餌料として用いて
ワムシを培養する（増殖させる）方法、或いは予めタウ
リンを取り込ませたクロレラおよび／または酵母を餌料
として用いてワムシを培養する（増殖させる）方法が好
ましい。これらの方法を採用した場合は、ワムシの体内
にタウリンが良好に取り込まれると共に、その他の栄養
面にも優れた、餌料価値の高いタウリン強化ワムシが生
産される。The method for producing the taurine-enriched rotifer of the present invention is not particularly limited, and any method can be adopted as long as it can increase taurine content by accumulating taurine in the body of a rotifer. Among them, the taurine-enriched rotifer of the present invention, under the addition of taurine, chlorella, algae, yeast, a method of culturing (proliferating) rotifer using at least one kind of fish egg powder as a feed, or taurine in advance. A method of culturing (growing) a rotifer using the incorporated Chlorella and / or yeast as a feed is preferable. When these methods are adopted, taurine is well incorporated into the body of the rotifer, and taurine-enriched rotifer with excellent nutritional value and high food value is produced.
【００１６】本発明のタウリン強化ワムシの生産は、ク
ロレラ、藻類、酵母、魚卵粉末などの少なくとも１種を
用いてワムシを１次培養する際にタウリンを強化して魚
介類に給餌する方法によって行ってもよいし、或いはク
ロレラ、藻類、酵母、魚卵粉末などの少なくとも１種を
用いてワムシを一次培養した後にω３ＨＵＦＡなどを給
与して２次強化する際にタウリンを強化する方法によっ
て行ってもよい。ワムシを魚介類に給餌する２４時間以
内にワムシ体内のタウリン含量を増強して強化すること
が望ましい。The production of the taurine-enriched rotifer of the present invention is carried out by a method in which taurine is enriched and fed to seafood when the rotifer is primarily cultured using at least one kind of chlorella, algae, yeast, fish egg powder and the like. May be done, or by culturing rotifer using at least one kind of chlorella, algae, yeast, roe powder, etc., and then feeding ω3HUFA etc. to strengthen taurine when secondary strengthening Good. It is desirable to enhance and strengthen the taurine content within the rotifer within 24 hours of feeding the rotifer to seafood.
【００１７】本発明のタウリン強化ワムシを生産する際
のタウリンの添加量は、ワムシの体内に蓄積含有させよ
うとするタウリン量などに応じて異なり得るが、一般的
には、ワムシ１億個体に対して０．５〜５０ｇ程度であ
ることが好ましく、１〜３０ｇ程度であることがより好
ましい。タウリンを添加したクロレラ、藻類、酵母、魚
卵粉末などのうちの少なくとも１種を用いてワムシの増
殖を行うに当たっては、各々の状況に応じて適した条件
を採用することができるが、クロレラを用いて増殖する
場合は、一般的には水温１８〜２８℃、培養（増殖）時
のワムシ密度３００〜５０００個体／ｍｌ、クロレラ
（生クロレラ）の給餌量１〜２０ｍｌ／リットルの条件
下に、２〜４８時間培養することにより、目的とするタ
ウリン強化ワムシを円滑に生産することができる。The amount of taurine to be added when the taurine-enriched rotifer of the present invention is produced may vary depending on the amount of taurine to be accumulated and contained in the body of the rotifer, etc. On the other hand, it is preferably about 0.5 to 50 g, and more preferably about 1 to 30 g. In growing rotifers using at least one of taurine-containing chlorella, algae, yeast, roe powder, etc., conditions suitable for each situation can be adopted. When grown using, generally under the conditions of water temperature 18 to 28 ° C, rotifer density during culture (propagation) 300 to 5000 individuals / ml, and chlorella (raw chlorella) feed amount 1 to 20 ml / liter, The desired taurine-enriched rotifer can be smoothly produced by culturing for 2 to 48 hours.
【００１８】上記したタウリン強化ワムシの生産時に、
ワムシ培養用のクロレラなどの餌料にタウリンと共に水
溶性ビタミン、特にビタミンＢ1および／またはビタミ
ンＣを添加すると、水溶性ビタミン、特にビタミンＢ1
および／またはビタミンＣの体内での含有量の高い、栄
養的に一層優れるタウリン強化ワムシを得ることができ
る。水溶性ビタミンのうち、ビタミンＢ1およびビタミ
ンＣは、他の水溶性ビタミンに比べて、ワムシ体内への
取り込み・蓄積性に優れている。タウリン強化ワムシに
おけるビタミンＢ1および／またはビタミンＣなどの含
有量は特に制限されないが、ワムシの体重に基づいて、
ビタミンＢ1では０．０５ｍｇ質量％以上、またビタミ
ンＣでは５ｍｇ質量％以上であることが、タウリン強化
ワムシの餌料として栄養面から好ましい。During the production of the above-mentioned taurine-enriched rotifer,
When water-soluble vitamins, especially vitamin B 1 and / or vitamin C, are added together with taurine to a diet such as chlorella for rotifer culture, water-soluble vitamins, especially vitamin B 1
It is possible to obtain a taurine-enriched rotifer that has a high content of vitamin C and / or vitamin C in the body and is nutritionally superior. Among the water-soluble vitamins, vitamin B 1 and vitamin C are superior to other water-soluble vitamins in their ability to be taken up and accumulated in the rotifer body. The content of vitamin B 1 and / or vitamin C in the taurine-fortified rotifer is not particularly limited, but based on the weight of the rotifer,
From the viewpoint of nutrition, a vitamin B 1 content of 0.05 mg mass% or more and a vitamin C content of 5 mg mass% or more are preferable from the viewpoint of nutrition as a feed for taurine-fortified rotifers.
【００１９】本発明のタウリン強化ワムシの生産に当た
っては、ワムシの２次強化に、必要に応じて、例えば、
クロレラ工業株式会社製「生クロレラω３」、日清サイ
エンス株式会社製「マリングロス」などのワムシ強化
剤、魚卵粉末などを添加して培養（増殖）を行うことが
できる。In the production of the taurine-enriched rotifer of the present invention, the rotifer is secondarily fortified, if necessary, for example, by:
Culture (proliferation) can be carried out by adding rotifer enhancers such as "raw chlorella ω3" manufactured by Chlorella Industry Co., Ltd. and "Maringros" manufactured by Nisshin Science Co., Ltd., fish egg powder and the like.
【００２０】本発明のタウリン強化ワムシは、魚介類を
飼育する際の生物餌料として有効に用いることができ、
特に海産魚介類の種苗生産時に孵化した仔稚魚に給与す
るための生物餌料として有用である。魚介類に給餌する
に当たっては、本発明のタウリン強化ワムシは、生きた
ままの状態で給餌しても、また場合によっては乾燥した
状態で給餌してもよく、特に生きたままで給餌すること
が好ましい。本発明のタウリン強化ワムシを給餌する魚
介類の種類は特に制限されず、従来からワムシを給餌し
て飼育することが行われている魚介類のいずれに対して
も有効に用いることができ、例えば、マダイ、ヒラメ、
トラフグ、ブリ、カンパチ、マグロなどの海産魚類、エ
ビ、カニなどの甲殻類、タコ、イカなどの頭足類などを
挙げることができる。魚介類への本発明のタウリン強化
ワムシの給餌期間は特に制限されず、魚介類の種類など
に応じて、従来と同様の期間にわたって給餌することが
できる。例えば、マダイに給餌する場合は孵化直後から
３０日令位まで、ヒラメに給餌する場合は孵化直後から
２５日令位まで給餌するとよい。The taurine-enriched rotifer of the present invention can be effectively used as a biological feed for raising seafood,
Particularly, it is useful as a biological feed for feeding larvae that have hatched during the production of seeds of marine seafood. In feeding seafood, the taurine-enriched rotifer of the present invention may be fed in a living state, or may be fed in a dried state in some cases, and it is particularly preferable to feed in a live state. . The type of fish and shellfish feeding the taurine-enriched rotifer of the present invention is not particularly limited, and can be effectively used for any of the fish and shellfish conventionally fed and bred with rotifers, for example, , Red sea bream, flounder,
Examples include marine fish such as trough, yellowtail, amberjack, and tuna, crustaceans such as shrimp and crab, and cephalopods such as octopus and squid. The period for feeding the taurine-enriched rotifer of the present invention to seafood is not particularly limited, and it can be fed for a period similar to the conventional one depending on the type of seafood. For example, when feeding red sea bream, it is advisable to feed immediately after hatching up to the 30th day of age, and when feeding flounder to the 25th day of age right after hatching.
【００２１】[0021]
【実施例】以下に実施例などにより本発明について具体
的に説明するが、本発明は以下の例により何ら限定され
るものではない。EXAMPLES The present invention will be specifically described below with reference to examples, but the present invention is not limited to the following examples.
【００２２】《実施例１》
（１） 継代培養したＳ型ワムシ約５億個体を、海水５
００リットルを収容した培養タンク（タンク容量６００
リットル）に入れ、そこに淡水クロレラである生クロレ
ラＶ１２（クロレラ工業株式会社製）を１日当たり４０
０ｍｌおよび海洋酵母（日清サイエンス株式会社製）を
１日２回、１回当たり８０ｇ（ワムシ１億個体当たり１
６ｇ）の割合で給餌して、水温２５℃で２日間培養し
た。
（２） ３つのタンク（各タンクの容量５０リットル）
を用意し、それぞれのタンクに海水３５リットルずつ入
れ、そこに上記（１）で培養したワムシを約1億２６０
０万個体／1タンクの割合で入れ（各タンクのワムシ密
度約３６００個体／ｍｌ）、水温２３〜２４℃で１６時
間培養した。その際に第１のタンク（試験区１：対照
区）ではタウリンを添加せずに市販のワムシ強化剤（ク
ロレラ工業株式会社製「生クロレラω３」）８０ｍｌの
みを添加して培養を行い、第２タンク（試験区２：発明
区）では前記ワムシ強化剤（生クロレラω３）８０ｍｌ
とタウリン（和光純薬株式会社製アミノエチルスルホン
酸）を１０ｇ添加して培養を行い、また第３タンク（試
験区３：発明区）では前記ワムシ強化剤８０ｍｌと前記
タウリン２０ｇを添加して培養を行った。
（３） 培養後に、それぞれのタンクからワムシを全量
取り出して、十分に水道水にて洗浄した後、ワムシ体内
に含まれているタウリン含量を測定してところ、下記の
表１に示すとおりであった。またワムシを温度１３５℃
で常圧加熱乾燥にて水分含量が１０質量％程度になるま
で乾燥して乾燥物（乾燥ワムシ）中に含まれるタウリン
含量を測定したところ、下記表１に示すとおりであっ
た。なお、ワムシ（生ワムシ）の体内でのタウリン含量
および乾燥ワムシ中のタウリン含量は、加水分解した
後、アミノ酸自動分析法により分析した。Example 1 (1) About 500 million S-type rotifers subcultured were treated with 5 times of seawater.
Culture tank containing 00 liters (tank capacity 600
Liter), and fresh chlorella V12 (manufactured by Chlorella Industry Co., Ltd.), which is fresh water chlorella, is added thereto 40 times a day.
0 ml and marine yeast (manufactured by Nisshin Science Co., Ltd.) twice a day, 80 g per time (1 per 100 million rotifers)
6 g) was fed and cultured at a water temperature of 25 ° C. for 2 days. (2) Three tanks (each tank has a capacity of 50 liters)
We prepared 35 liters of seawater in each tank and put about 120 million rotifers cultured in (1) above.
It was placed at a rate of 0,000 individuals / tank (rotifer density of each tank was about 3600 individuals / ml), and cultured at a water temperature of 23 to 24 ° C for 16 hours. At that time, in the first tank (test section 1: control section), without adding taurine, only 80 ml of a commercially available rotifer enhancer (“fresh chlorella ω3” manufactured by Chlorella Industry Co., Ltd.) was added and cultured, 80 ml of the rotifer enhancer (raw chlorella ω3) in 2 tanks (test zone 2: invention zone)
And taurine (aminoethyl sulfonic acid manufactured by Wako Pure Chemical Industries, Ltd.) were added for culturing, and in the third tank (test section 3: invention section), 80 ml of the rotifer enhancer and 20 g of the taurine were added and cultivated. I went. (3) After culturing, all the rotifers were taken out of each tank, washed thoroughly with tap water, and the taurine content in the rotifer was measured. The results are shown in Table 1 below. It was Rotate the rotifer at a temperature of 135 ° C.
The content of taurine contained in the dried product (dried rotifer) was measured by drying under normal pressure heating and drying until the water content became about 10% by mass. The results are shown in Table 1 below. The taurine content in the body of the rotifer (raw rotifer) and the taurine content in the dried rotifer were hydrolyzed and then analyzed by the amino acid automatic analysis method.
【００２３】[0023]
【表１】 [Table 1]
【００２４】《実施例２》
（１） 実施例１の（２）において、試験区２ではタウ
リン１０ｇと共にビタミンＢ1硝酸塩（和光純薬工業株
式会社製）を１５ｍｇ、試験区３ではタウリン２０ｇと
共に前記ビタミンＢ1硝酸塩を３０ｍｇの割合で添加し
た以外は、実施例１の（１）および（２）と同じ操作を
行ってワムシの培養（増殖）を行った。
（２） 培養後に、それぞれのタンクからワムシを全量
取り出して、十分に水道水にて洗浄した後、ワムシ体内
に含まれているタウリン含量およびビタミンＢ1含量を
測定してところ、下記の表２に示すとおりであった。ま
た、ワムシを温度１３５℃にて常圧加熱乾燥法により、
ワムシの水分を測定し、これよりワムシ乾燥質量当りに
含まれるビタミンＢ1含量を測定したところ、下記表２
に示すとおりであった。なお、ワムシ中（生ワムシ）体
内でのタウリン含量および乾燥ワムシ中のタウリン含量
は実施例１と同様にして測定した。また、ワムシ（生ワ
ムシ）体内中のビタミンＢ1含量は、サイアミン塩酸塩
として高速液体クロマトグラフ法により測定した。Example 2 (1) In Example 1 (2), in test section 2, taurine 10 g and vitamin B 1 nitrate (manufactured by Wako Pure Chemical Industries, Ltd.) 15 mg, and in test section 3 taurine 20 g The rotifer was cultivated (proliferated) by the same procedure as in (1) and (2) of Example 1, except that the vitamin B 1 nitrate was added at a ratio of 30 mg. (2) After culturing, all rotifers were taken out from each tank, washed thoroughly with tap water, and then the taurine content and vitamin B 1 content contained in the rotifer were measured. It was as shown in. In addition, the rotifer is dried at a temperature of 135 ° C. under atmospheric pressure and dried,
The water content of the rotifer was measured, and the content of vitamin B 1 contained in the dry mass of the rotifer was measured.
It was as shown in. The taurine content in the rotifer (raw rotifer) and the taurine content in the dried rotifer were measured in the same manner as in Example 1. The vitamin B 1 content in the body of rotifer (raw rotifer) was measured by high performance liquid chromatography as thiamine hydrochloride.
【００２５】[0025]
【表２】 [Table 2]
【００２６】《実施例３》
（１） 実施例１の（２）において、試験区２ではタウ
リン１０ｇと共にビタミンＣカルシウム塩（Ｊangshan
Ｐharmaceutical Ｃｏ．Ｌtd．）を１０ｇ、試験区３で
はタウリン２０ｇと共に前記ビタミンＣカルシウム塩を
２０ｇの割合で添加した以外は、実施例１の（１）およ
び（２）と同じ操作を行ってワムシの培養（増殖）を行
った。
（２） 培養後に、それぞれのタンクからワムシを全量
取り出して、十分に水道水にて洗浄した後、ワムシ体内
に含まれているタウリン含量およびビタミンＣ含量を測
定してところ、下記の表３に示すとおりであった。ま
た、ワムシを温度１３５℃にて常圧加熱乾燥法により、
ワムシの水分を測定し、これよりワムシ乾燥質量当りに
含まれるビタミンＣ含量を測定したところ、下記表３に
示すとおりであった。なお、ワムシ中（生ワムシ）体内
でのタウリン含量および乾燥ワムシ中のタウリン含量は
実施例１と同様にして測定した。また、ワムシ（生ワム
シ）体内中のビタミンＣ含量は、ヒドラジンで誘導体化
した後、総アスコルビン酸として高速液体クロマトグラ
フ法により測定した。Example 3 (1) In (2) of Example 1, in test section 2, 10 g of taurine and vitamin C calcium salt (Jangshan
Pharmaceutical Co. Ltd. 10 g), and in test section 3 except that the vitamin C calcium salt was added at a rate of 20 g together with 20 g of taurine, the same operation as in (1) and (2) of Example 1 was performed to culture (proliferate) the rotifer. went. (2) After culturing, all the rotifers were taken out from each tank, washed thoroughly with tap water, and the taurine content and vitamin C content contained in the rotifer were measured. It was as shown. In addition, the rotifer is dried at a temperature of 135 ° C. under atmospheric pressure and dried,
The water content of the rotifer was measured, and the content of vitamin C contained in the dried rotifer was measured. The results are shown in Table 3 below. The taurine content in the rotifer (raw rotifer) and the taurine content in the dried rotifer were measured in the same manner as in Example 1. The vitamin C content in the body of rotifer (raw rotifer) was measured by high performance liquid chromatography as total ascorbic acid after derivatization with hydrazine.
【００２７】[0027]
【表３】 [Table 3]
【００２８】《実施例４》
（１） 孵化直後のヒラメ仔稚魚を２区［Ａ区（対照
区）およびＢ区（発明区）］に分け（各区約４万個
体）、それぞれを５００リットルパンライト水槽に収容
し、水温１８℃にて飼育した。
（２） Ａ区のヒラメ仔稚魚には、実施例１の試験区１
で得られたワムシ（タウリンを含有しない生ワムシ）を
１日２〜３回の割合で給与して飽食摂餌させて１７日間
飼育を行った。
（３）Ｂ区のヒラメ仔稚魚には、実施例１の試験区２で
得られたワムシ（タウリンを含有する生ワムシ）を１日
２〜３回の割合で給与して飽食摂餌させて１７日間飼育
を行った。
（４）（ｉ） 上記（２）または（３）の飼育試験にお
いて、飼育１７日後の斃死率を調べたところ、下記の表
４に示すとおりであった。
（ii） また、飼育１７日後におけるヒラメ仔稚魚の平
均全長（頭の先から尾の先まで）を測定したところ下記
の表４に示すとおりであった。
（iii） さらに、飼育１７日後のヒラメ仔稚魚の活力
を下記の方法で調べたところ、以下の表４の示すとおり
であった。Example 4 (1) Flounder flounder larvae immediately after hatching are divided into 2 wards [A ward (control ward) and B ward (invention ward)] (about 40,000 individuals in each ward), each of which is a 500-liter bread It was housed in a light water tank and kept at a water temperature of 18 ° C. (2) For the flatfish larvae in the A ward, the test ward 1 in Example 1 was used.
The rotifer (raw rotifer containing no taurine) obtained in 1. was fed at a rate of 2 to 3 times a day, fed satiety and fed for 17 days. (3) For the flounder larvae of the B ward, the rotifer (raw rotifer containing taurine) obtained in the test ward 2 of Example 1 was fed at a rate of 2 to 3 times a day to allow it to be fed satiety. The animals were raised for 17 days. (4) (i) In the breeding test of (2) or (3) above, the mortality rate after 17 days of breeding was examined and the results are shown in Table 4 below. (Ii) Further, the average total length (from the tip of the head to the tip of the tail) of the flounder larvae after 17 days of breeding was measured, and the results are shown in Table 4 below. (Iii) Furthermore, when the vitality of the flounder fry after 17 days of breeding was examined by the following method, it was as shown in Table 4 below.
【００２９】［ヒラメ仔稚魚の活力の評価方法］タモ網
にて飼育１７日後のヒラメ仔稚魚をすくって（約１００
尾）、空中に２０秒間露出させた後、飼育海水に戻し、
その１５分後の生存率を求めて、生存率の高低によって
仔稚魚の活力の大小の評価を行った。[Evaluation Method of Vitality of Flounder Fry] Flounder larvae after 17 days of rearing are scooped with Tamo net (about 100).
Tail), exposed to air for 20 seconds, then returned to breeding seawater,
After 15 minutes, the survival rate was obtained, and the vitality of the larvae was evaluated based on the level of the survival rate.
【００３０】[0030]
【表４】 [Table 4]
【００３１】上記の表４にみるように、タウリンを含有
する試験区２のワムシ（タウリン強化ワムシ）を給餌し
たＢ区は、タウリンを含有しない試験区１のワムシを給
餌したＡ区に比べて、１７日間の飼育期間中、ヒラメ仔
稚魚の斃死率が大幅に低減しており、しかも空気中で２
０秒間露出してから海水に戻した後の生存率が高く、活
力に優れている。As shown in Table 4 above, the group B fed with the rotifer of the taurine-containing test group 2 (taurine-enriched rotifer) was compared with the group A fed with the rotifer of the test group 1 containing no taurine. , The mortality rate of juvenile flounder larvae has been significantly reduced during the 17-day breeding period, and 2 in the air.
It has a high survival rate after being exposed for 0 seconds and then returned to seawater, and has excellent vitality.
【００３２】《実施例５》
（１） 孵化直後のマダイ仔稚魚を２区［Ａ区（対照
区）およびＢ区（発明区）］に分け（各区約４万個
体）、それぞれを５００リットルパンライト水槽に収容
し、水温１８℃にて飼育した。
（２） Ａ区のマダイ仔稚魚には、実施例１の試験区１
で得られた生ワムシ（タウリンを含有しない生ワムシ）
を１日２〜３回の割合で給与して飽食摂餌させて１７日
間飼育を行った。
（３）Ｂ区のマダイ仔稚魚には、実施例１の試験区２で
得られた生ワムシ（タウリンを含有する生ワムシ）を１
日２〜３回の割合で給与して飽食摂餌させて１７日間飼
育を行った。
（４）（ｉ） 上記（２）または（３）の飼育試験にお
いて、飼育１７日後の斃死率を調べたところ、下記の表
４に示すとおりであった。
（ii） また、飼育１７日後におけるマダイ仔稚魚の平
均全長（頭の先から尾の先まで）を測定したところ下記
の表５に示すとおりであった。
（iii） さらに、飼育１７日後のマダイ仔稚魚の活力
を下記の方法で調べたところ、以下の表５の示すとおり
であった。Example 5 (1) Frying red sea bream larvae immediately after hatching are divided into 2 wards [A ward (control ward) and B ward (invention ward)] (about 40,000 individuals in each ward), each of which is a 500-liter bread It was housed in a light water tank and kept at a water temperature of 18 ° C. (2) For the juvenile red sea bream in the A ward, the test ward 1 in Example 1 was used.
Raw rotifer obtained in step (raw rotifer without taurine)
Was fed at a rate of 2 to 3 times a day to allow it to be fed satiety and fed for 17 days. (3) For the juvenile red sea bream in the B ward, 1 rotifer (raw rotifer containing taurine) obtained in the test ward 2 of Example 1 is used.
They were fed at a rate of 2 to 3 times a day, fed satiety, and fed for 17 days. (4) (i) In the breeding test of (2) or (3) above, the mortality rate after 17 days of breeding was examined and the results are shown in Table 4 below. (Ii) Further, the average total length (from the tip of the head to the tip of the tail) of the juvenile red sea bream after 17 days of breeding was measured, and the results are shown in Table 5 below. (Iii) Furthermore, when the vitality of the juvenile red sea bream after 17 days of breeding was examined by the following method, it was as shown in Table 5 below.
【００３３】［マダイ仔稚魚の活力の評価方法］タモ網
にて飼育１７日後のマダイ仔稚魚をすくって（約１００
尾）、空中に５秒間露出させた後、飼育海水に戻し、そ
の１５分後の生存率を求めて、生存率の高低によって仔
稚魚の活力の大小の評価を行った。[Method of evaluating vitality of red sea bream larvae] Frying red sea bream larvae after 17 days of rearing with a tamo net (about 100)
Tail), exposed to the air for 5 seconds, returned to the breeding seawater, and after 15 minutes, the survival rate was determined and the vitality of the larvae was evaluated based on the level of survival rate.
【００３４】[0034]
【表５】 [Table 5]
【００３５】上記の表５にみるように、タウリンを含有
する試験区２のワムシ（タウリン強化ワムシ）を給餌し
たＢ区は、タウリンを含有しない試験区１のワムシを給
餌したＡ区に比べて、１７日間の飼育期間中、マダイ仔
稚魚の斃死率が大幅に低減しており、しかも空気中で５
秒間露出してから海水に戻した後の生存率が高く、活力
にも優れている。As shown in Table 5 above, the B group fed with the rotifer containing taurine in the test group 2 (taurine-enriched rotifer) was compared with the A group fed with the rotifer in the test group 1 containing no taurine. , The mortality rate of juvenile red sea breams has been significantly reduced during the 17-day breeding period, and the mortality rate was 5 in the air.
It has a high survival rate after being exposed for a second and then returned to seawater, and has excellent vitality.
【００３６】[0036]
【発明の効果】本発明のタウリン強化ワムシを用いて魚
介類の仔稚魚を飼育すると、斃死率が低下すると共に、
仔稚魚の活力の増加および生育促進を図ることができ、
そのため本発明のタウリン強化ワムシは、魚介類の飼
育、特に種苗生産に有効に使用することができる。ワム
シの体内にタウリンと共に更に水溶性ビタミン、特にビ
タミンＢ1および／またはビタミンＣを含有する本発明
のタウリン強化ワムシは、魚介類に対する栄養面で一層
優れており、魚介類、特に仔稚魚を、低い斃死率で、し
かも仔稚魚の活力を増加させならが、良好に生育させる
ことができる。タウリンの添加、またはタウリンと水溶
性ビタミン、特にビタミンＢ1および／またはビタミン
Ｃの添加下に、クロレラ、藻類、酵母、魚卵粉末などの
うちの少なくとも１種を用いてワムシを培養する本発明
の方法による場合は、魚介類、特に仔稚魚の飼育に有用
な上記タウリン強化ワムシを円滑に生産することができ
る。EFFECTS OF THE INVENTION Rearing of larvae of seafood using the taurine-enriched rotifer of the present invention reduces the mortality rate and
It is possible to increase the vitality and promote the growth of larvae,
Therefore, the taurine-enriched rotifer of the present invention can be effectively used for breeding seafood, especially for seedling production. The taurine-enriched rotifer of the present invention containing a water-soluble vitamin, particularly vitamin B 1 and / or vitamin C, in addition to taurine in the body of the rotifer is more excellent in terms of nutrition for fish and shellfish. It has a low mortality rate and can grow well even if the vitality of larvae is increased. The present invention in which a rotifer is cultured by using at least one of chlorella, algae, yeast, fish egg powder, etc. under the addition of taurine, or taurine and a water-soluble vitamin, particularly vitamin B 1 and / or vitamin C. According to the method described above, the above taurine-enriched rotifer useful for breeding seafood, especially larvae, can be smoothly produced.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 竹内 俊郎 東京都港区港南４−５−７ 東京水産大学 水産学部内 Ｆターム(参考） 2B005 GA09 HA01 MB09 NA18 ─────────────────────────────────────────────────── ─── Continued front page (72) Inventor Toshiro Takeuchi 4-5-7 Konan, Minato-ku, Tokyo Tokyo Fisheries University Faculty of Fisheries F-term (reference) 2B005 GA09 HA01 MB09 NA18
Claims (9)
ことを特徴とするタウリン強化ワムシ。1. A taurine-enriched rotifer having an increased taurine content in the body.
０．０１質量％以上である請求項１に記載のタウリン強
化ワムシ。2. The taurine content relative to the body weight of a rotifer,
The taurine-fortified rotifer according to claim 1, which is 0.01% by mass or more.
が増強されている請求項１または２に記載のタウリン強
化ワムシ。3. The taurine-enriched rotifer according to claim 1 or 2, wherein the content of water-soluble vitamins in the body is further enhanced.
／またはビタミンＣである請求項３に記載のタウリン強
化ワムシ。4. The taurine-enriched rotifer according to claim 3, wherein the water-soluble vitamin is vitamin B 1 and / or vitamin C.
溶性ビタミンの添加下に、クロレラ、藻類、酵母および
魚卵粉末のうちの少なくとも１種を用いて培養された請
求項１〜４のいずれか１項に記載のタウリン強化ワム
シ。5. The method according to any one of claims 1 to 4, which is cultivated using at least one of chlorella, algae, yeast and fish egg powder under the addition of taurine or the addition of taurine and a water-soluble vitamin. A taurine-enriched rotifer according to item 1.
か１項に記載のタウリン強化ワムシ。6. The taurine-enriched rotifer according to claim 1, which is a feed for fish farming.
性ビタミンの添加下に、クロレラ、藻類、酵母および魚
卵粉末のうちの少なくとも１種を用いてワムシを培養す
ることを特徴とする、体内でのタウリン含量、またはタ
ウリン含量と水溶性ビタミン含量の増強されたタウリン
強化ワムシの生産方法。7. A rotifer is cultivated in the body using at least one of chlorella, algae, yeast and fish egg powder under the addition of taurine or taurine and a water-soluble vitamin. Of a taurine-enriched rotifer with enhanced taurine content, or taurine content and water-soluble vitamin content.
／またはビタミンＣである請求項７に記載の生産方法。8. The production method according to claim 7, wherein the water-soluble vitamin is vitamin B 1 and / or vitamin C.
ウリン強化ワムシを用いて魚介類を飼育する方法。9. A method for breeding seafood using the taurine-enriched rotifer according to any one of claims 1 to 6.
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|---|---|---|---|
| JP2002065682A JP2003259760A (en) | 2002-03-11 | 2002-03-11 | Taurine-enriched rotifers |
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ID=28671300
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007053967A (en) * | 2005-08-24 | 2007-03-08 | Oriental Yeast Co Ltd | Fodder for enriching taurine in animal fodder |
| CN102511432A (en) * | 2012-01-13 | 2012-06-27 | 广东海洋大学 | Method for cultivating Brachionus plicatilis |
| CN108124796A (en) * | 2018-01-08 | 2018-06-08 | 广西壮族自治区海洋研究所 | A kind of method for cultivating wheel animalcule |
-
2002
- 2002-03-11 JP JP2002065682A patent/JP2003259760A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007053967A (en) * | 2005-08-24 | 2007-03-08 | Oriental Yeast Co Ltd | Fodder for enriching taurine in animal fodder |
| CN102511432A (en) * | 2012-01-13 | 2012-06-27 | 广东海洋大学 | Method for cultivating Brachionus plicatilis |
| CN108124796A (en) * | 2018-01-08 | 2018-06-08 | 广西壮族自治区海洋研究所 | A kind of method for cultivating wheel animalcule |
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