KR102164339B1 - feed composition for phyllosoma larvae of spiny lobster - Google Patents

Abstract

The present invention relates to a feed composition for spiny lobster larvae from phyllosoma to juvenile steps and a spiny lobster larva breeding method using the same. With the feed composition for spiny lobster larvae of the present invention, the survival rate of larvae from the phyllosoma to juvenile steps can be substantially improved and an excellent growth enhancement effect can be achieved. Accordingly, the feed composition of the present invention can be useful for artificial lobster larva breeding.

Description

Feed composition for phyllosoma larvae of spiny lobster}

The present invention relates to a feed composition for spiny lobster larvae from phyllosoma to juvenile larvae, and a method for rearing larvae using the same.

In the global sea shrimp market, lobsters with large tongs (Nephropoidea) account for 66%, and spiny lobsters (Palinuroidea), which will be farmed through feed made through this technology development, account for 32% of the world. do.

Spa am mainly lobster and reef habitat in the area and carrying mud (沼池), the English 'Spiny lobster' and the scientific name is' Panulirus depending on the local habitat cygnus , Panulirus ornatus, Panulirus argus , Panulirus laevicauda , Panulirus interruptus , Panulirus polyphagus , Panulirus homarus , Panulirus japonicus ' species. The two breastplates or the exoskeleton of the abdomen are very hard. The head column is close to a cylinder shape, and the belly is flat. There are many forward-facing thorns on the back of the two breastplates, the larger the one in front. There are no forehead horns, and there is a pair of large anterior thorns on the forehead. The tail is rectangular, 1.7 times longer than the butterfly, and the corners of the back edge are rounded. The antennae is very thick and long, more than twice the length of the body, and there are large thorns on the antennal stalk, many small thorns on the whip, and the sounder at the inner base of the antennae stalk. In Korea, some of the similar species are caught on Jeju Island, but most of them are farmed on the east coast of Vietnam.

Spiny lobster transforms into a puerulus larvae that grows to about 30 mm in length as it grows in the phyllosoma larvae of about 1.5 mm in size immediately after hatching from an egg. ) Go through the larval stage.

The demand for spiny lobster continues to increase around the world, and the demand for lobster is increasing rapidly as the increasing demand of the middle class in Asia is increasing. To meet this demand, mainly in Southeast Asia. Soma or Puerulus) is collected in nature and then farmed.

However, in the field of fisheries test research, phyllosoma larvae are artificially hatched and then cultured around egg yolk as a food source, but it is difficult to grow and rear due to high mortality and deteriorating water quality. It's just that individual breeding is being done. The causes of difficulty in artificial rearing of phyllosoma larvae include the physical characteristics required for feed feeding, the peculiarity of the larvae's benthic behavior and floating life, and contamination by microorganisms caused by feces or debris at the bottom of the tank. , Palatability for chemical substances that are diffused in the process of attracting food, and securing areas due to individual interference due to food feeding activities, although they lead a community life.

Meanwhile, some studies have previously reported that the nutritional requirements of the spiny lobster at the early stage of growth are very important. In terms of nutritional requirements, if the imbalance required for growth and molting is not resolved, mortality due to emptiness occurs due to the survival competition for food after the Puerulus stage, and due to the nature of crustaceans, the growth process In the case of lack of nutritional requirements during the peeling process necessary for, a large amount of death occurs.

Therefore, in order to increase the growth and survival rate in spy lobster farming, the demand for compound feed to be supplied to larvae from phyllosoma to juvenile larvae among the growth stages is increasing day by day.

Under this background, the present inventors not only reduce the mortality rate of spiny lobster larvae, but also increase the mortality rate of spiny lobster larvae when feeding feed containing a specific component as a protein source to larvae from phyllosoma to juvenile larvae. The present invention was completed by confirming the effect of improving the weight gain.

Korean Patent Registration No. 10-0780907

Therefore, it is to provide a feed composition that can effectively lead to growth while improving the survival rate of the spiny lobster larvae from the phyllosoma to the juvenile larvae of the present invention.

Another object of the present invention is to provide a method for effectively breeding sphiny lobster larvae from phyllosoma to juvenile larvae using the composition.

In order to achieve the object of the present invention as described above,

The present invention provides a feed composition for spiny lobster larvae comprising one or more raw materials selected from the group consisting of mackerel, herring, mussels and crabs as an active ingredient.

In one embodiment of the present invention, the raw material may be included in an amount of 5 to 30 parts by weight based on 100 parts by weight of the total composition.

In one embodiment of the present invention, the raw material is mackerel or herring; mussel; And crabs.

In one embodiment of the present invention, the mackerel or herring; mussel; And crab may be included in a weight ratio of 1:1:1.

In one embodiment of the present invention, the spiny lobster larvae may be larvae ranging from phyllosoma to juvenile.

In one embodiment of the present invention, the composition is squid, fish meal, krill meal, wheat flour, seaweed, fish oil, gluten, glycine, arginine, lysine hydrochloride, methionine, cysteine hydrochloride, betaine, lecithin, choline chloride, vitamin complex , Mineral complexes, TGA (Transglutaminase), sodium caseinate, rotifer culture solution, spirulina, kiwi, sodium alginate, guar gum, crab flavor, and at least one selected from the group consisting of podzol may be further included.

In addition, the present invention provides a method of breeding a larvae of a spiny lobster, comprising the step of feeding the composition to a larvae of a spiny lobster.

When using the feed composition for spiny lobster larvae of the present invention, the survival rate of larvae from phyllosoma to juvenile can be remarkably improved, and the growth enhancing effect is excellent. Therefore, the feed composition of the present invention can be usefully used in artificial breeding of shrimp and lobster larvae.

1 is a schematic diagram showing a simplified process of manufacturing a wet feed according to the present invention.
Figure 2 is a schematic diagram showing a schematic diagram showing a soft feed manufacturing process according to the present invention.
Figure 3 is a photograph showing the spiny lobster larvae wet feed and soft feed of the present invention.
Figures 4a to 4c are photographs showing the spiny lobster larvae breeding tank used in the following experiment of the present invention.
Figure 5 Panulirus The initial size of ornatus lobster shows the effect of pellet feeding on growth.

The present invention relates to a feed composition for spiny lobster larvae.

Currently, spiny lobster larvae are farmed mainly on catch fish, snails, and shellfish, but these food sources have nutritional imbalances depending on the type and quality, showing low survival rates and slow growth rates of around 30%.

As an invention conceived to solve the above problems of the present invention, mortality of spiny lobster larvae when feed containing a specific component as a protein source is supplied to larvae from phyllosoma to juvenile In addition to reducing the effect of improving the weight gain was confirmed.

The composition of the present invention is characterized in that it contains one or more raw materials selected from the group consisting of mackerel or herring, mussels and crabs as an active ingredient.

Mackerel or herring used as a raw material of the present invention was used except for the head, intestines, and fins, and the mussel was used only for the edible part excluding the shell. Fresh raw materials can be pulverized at low temperatures to prevent rupture of nutrients and bioactive substances.

In addition to mackerel or herring, mussels, and crabs as the raw material of the present invention, squid (only the body part excluding the intestines and legs is crushed and then stored frozen).

The raw material of the present invention can be used in 5 to 30% to be useful for digestion and absorption by reflecting the nutritional requirements.

In one embodiment of the present invention, the raw material may include all of mackerel, mussels and crabs, and in this case, mackerel, mussels and crabs may be included in a weight ratio of 1:1:1.

The composition of the present invention may contain fish meal and krill meal in order to overcome the limitation of deficiencies of various nutrients required in the process of growth and peeling, and may contain 20 to 50% of these mixtures.

The composition of the present invention is a lipid source in addition to raw materials; Amino acid source; Carbohydrate source and ingredient adhesives; It may further include other additives for physiological activity and immunity enhancement.

As the lipid source, fish oil and olive oil may be used to supplement a nutrient source required for growth, and the composition may contain 1 to 5% fish oil and olive oil, respectively.

As the amino acid source, glycine, arginine, lysine hydrochloride, methionine, cysteine hydrochloride, and betaine may be used to supplement the amino acid component required for growth and increase attractiveness, and proline, alanine, taurine, etc. may be used as necessary. . Glycine, which is a component of hormones such as oxytocin, plays an important role in metabolism in vivo, and arginine, which plays an important role in muscle contraction and affects appetite and growth rate, is used to match the composition ratio because it has a hostile relationship with arginine. Lysine hydrochloride, which is a scent component, has affinity with fat, and is used as cysteine hydrochloride to complement cysteine, which is involved in collagen production and choline synthesis, and cysteine involved in the production of lipoic acid. Each of betaine and the like may be contained in an amount of 0.1 to 3%.

As the carbohydrate source and ingredient adhesive, wheat flour, α-starch, gluten, sodium caseinate, sodium alginate, and guar gum can be used, and if necessary, glycerin fatty acid ester, TGA (transglutaminase), proline, carrageenan, agar, etc. can be used. have. For use as a source of carbohydrates used as an active energy source and an ingredient adhesive for maintaining the formulation, wheat flour and α-potato starch may be included in the composition in an amount of 3 to 15%, and for use as a thickener or stabilizer in feed, gluten and casein Sodium, sodium alginate, guar gum, etc. may be contained in an amount of 0.1 to 4%, respectively.

Other additives for the physiological activity and immunity enhancement include rotifer culture solution, seaweed, spirulina (adapted as a food source in the phylosoma and puerulus stages), vitamin mixture, mineral mixture, podzol, phytase and kiwi (digestion rate Promotion and intestinal development), probiotics (such as Bacillus subtilis and Lactobacillus plantarum), fructooligosaccharide (enhances beneficial bacteria in the intestine), lecithin (solubilizes cholesterol constituting the biological membrane), choline chloride, and can be used. If necessary, 0.1 to 5% of altemia, copepods, kelp meal, vitamin C and vitamin E, calcium salts and phosphates, and crab flavors can be used to improve palatability, respectively.

In order to prepare a rotifer culture medium used as needed, 50 mL of a commercially available frozen rotifer culture medium may be thawed at room temperature and used as a bacterial inoculum. As a substrate source for the rotifer, 2L of spirulina was prepared in a 5L Air-Lift Bioreactor container with 1g/L of spirulina, 20 g/L of sea salt, and 2L of the culture solution by setting the initial pH to 7. It can be used after incubation for 10 days while supplying.

In one embodiment of the present invention, the composition may be used as a feed composition for breeding spiny lobster larvae from phyllosoma to puerulus.

The composition of the present invention may be a wet feed composition, and the manufacturing process of the wet feed is as follows.

Ⅰ. In the raw material trimming and pulverization step, mackerel and herring excluding head, intestines and fins, small crabs are frozen, and mussels are mixed and crushed according to the raw material composition ratio.

Ⅱ. Mixing after quantification of solid raw materials and powder additives.

Ⅲ. Mix and knead A and B with liquid additives (fish oil and kiwi juice).

IV. Olive oil coating

In another embodiment of the present invention, the composition may be used as a feed composition for breeding spiny lobster larvae from the middle stage of puerulus to the middle stage of juvenile.

The composition of the present invention may be a soft feed composition, and the manufacturing process of the soft feed is as follows.

Steps Ⅰ to Ⅳ are the same as the wet feed

Ⅴ. Add appropriate amount of moisture (5 ~ 10%)

Ⅵ. In order to prevent entanglement when formulated in pellet form, it is coated using pulverized grains with fibrous surface.

Ⅶ. 45℃, drying for 2 to 4 hours

In addition, the present invention provides a method of breeding a larvae of a spiny lobster, comprising the step of feeding the composition to a larvae of a spiny lobster.

In the process of feeding the feed required for fish farming of the larvae of the spiny lobster, it is desirable to perform the number of feeding twice a day, and observe the amount eaten after feeding the previous day, and observe the amount eaten after feeding the next day (1 /2, 1/2), (1/3, 2/3), (1/4, 3/4), (1/5, 4/5) can be distributed and fed.

Hereinafter, the present invention will be described in more detail through examples. These examples are for explaining the present invention more specifically, and the scope of the present invention is not limited to these examples.

< Example >

Preparation of Ingredients for Wet Feed Manufacturing

The mackerel and herring were frozen domestically; Mussels were used in Namhae and Tongyeong, Gyeongsangnam-do; Crabs were frozen from China; For squid, squid agitated from the east coast was used; Fish meal (Jack Mackerel) was used ALIMENTOS MARINOS S. A. Chile, and krill meal was purchased from Dongwon Fisheries; Wheat flour was purchased from CJ CheilJedang; α-starch was used as THAI ASAHI, Thailand; Seaweed (seaweed powder) used Palms Road (raw material domestically), and fish oil (sardine oil) was purchased from Daekyung ONT Co., Ltd. (origin: Russia); Gluten (active wheat gluten) was purchased from Komida (country of origin France); For glycine, Japan, Arginine (L-Arginine Base), China was used; Lysine hydrochloride (L-Lysine HCl 99%) was used as PT.CHEILIEDANG Indonesia; Methionine (DL-Methionine) was used as SUMITOMO CHEMICAL, Japan; Cysteine hydrochloride (L-Cysteine Monohydrochloride 100%) was used as Samin Chemical Co., Ltd., Korea, betaine (Betaine anhydrous 97%), Danisco Animal Nutrition, UK was used; Lecithin (soy lecithin, granule 70%) was purchased from Sein International Co., Ltd. (origin Taiwan); Choline chloride (Choline Chloride 50%) was used by Kolin Chemical, Korea; Vitamin C was used as L-Ascorbate-2-Phosphate 35% YIXING JIANGSHAN BIO-TECH, China; The vitamin complex was purchased from Woosung Corporation, Korea; The mineral complex was purchased from Daeyoung Bio (Origin USA); TGA (Transglutaminase) was used by AJIMOTO CO, Japan; Sodium Caseinate was used LACTOPROT DEUTSCHLQAND GMBH, Germany; Rotifer culture solution was purchased from Bizidduk, Suncheon, Jeollanam-do; Spirulina was purchased from SNC International (Origin USA); Kiwi was used domestically and Chilean; Sodium Alginate was used QINDAO BRIGHT MOON SEAWEED GR, China; Guar Gum Powder, Shree Vijaylaxmi International, India was used; Mokkyung, Korea was used for Crab Flavor; For phytase, CTC Bio, ㆍKorea was used; Probiotics were purchased from Woosung Corporation, Korea; For fructo-oligosaccharide (Fructo-OligoSacchaaride 10%), Sensus B. V Netherlands was used; Calcium Lactate was used as Pentahydrate GALACTIC, USA; The first calcium phosphate (MonoCalcium Phosphate) was used as Guizhou Chanhen Chemical Corporation, China.

Feeding

Feeding was provided twice a day in the morning (within 2 hours after sunrise) and in the afternoon (within 2 hours after sunset), and the amount of feed for the next day in consideration of the amount of feed remaining after 2 hours of feeding. Was supplied.

The feed rate for the 1st to 5th experiments was supplied for the next day in consideration of the amount of feed remaining 2 hours after feeding. In the 6th experiment, 1/3 of the daily feed intake was fed in the morning and 2/3 was fed in the afternoon. In the 7th experiment, 1/5 of the daily feed intake was fed in the morning and 4/5 was fed in the afternoon. In the 8th and 9th experiments, 1/4 of the daily feed intake was fed in the morning and 3/4 was fed in the afternoon.

From the first to the ninth experiment, the feed from the beginning of puerulus was supplied as wet feed, and raw ingredients were prepared for the wet feed, and components other than the raw ingredients were mixed there and kneaded with a rolling roller. Was prepared (see Fig. 1). On the other hand, mackerel and sardines used as raw materials were used excluding heads, intestines and fins, and small crabs were frozen, and mussels were edible parts excluding skins. These were mixed and ground according to the composition ratio in each of the following examples. In the 7th experiment, dry food was supplied from the beginning of breeding, and in the 8th experiment, dry food was supplied from the late Puerulus.

Breeding tank

In one embodiment of the breeding experiment from phyllosoma to the initial stage of puerulus, a circular bucket (800L) having a diameter × height of 1m × 1m from above sea level is filled with seawater at a depth of 0.6m. An oxygen supply was installed to continuously supply oxygen. Using the nets used in the existing farms, experiments 1 to 6, and 8 and 9 experiments were raised in a sea cage pond 1m × 1m × 1m (width × length × height), and the 7th experiment. 2m × 2m × 2m was used to conduct mass loading experiments. In the 8th test, a frame was installed in one pond of 1m × 1m × 1m, and in the 9th test, the frame was installed in one pond of 1m × 1m × 1.5m. The frame was installed.

< Example 1>

Experiment 1st: Marine Edging In one nursing home (1m × 1m × 1m) Puerulus Results of farming 100 medium-sized animals for 23 days

Krill meal, frozen squid (soyster), peduncle, lobster, sodium alginate, agar, carrageenan, monobasic calcium phosphate (MCP), choline chloride, taurine, DL-alanine, glycine, betaine, brewer's yeast, vitamin complex, It was prepared in the form of a wet feed containing mineral complex, vitamin C, vitamin E, arginine, proline, and calcium lactate.

Due to its weak viscosity, it was observed that it was easily consumed as food for fish under the net, but 12 out of 100 puerulus died and 88 survived.

Experiment 1st feed composition (wt%) Ingredients Experimental group 1 Krill wheat 20 to 40 Frozen squid, peduncle, roe shrimp 30 to 50 Sodium alginate 0.1 to 4 Agar 0.1 to 4 Karakinan 0.1 to 4 MCP 0.1 to 4 Choline chloride 0.1 to 3 Taurine 0.1 to 3 DL-alanine 0.1 to 3 Glycine 0.1 to 3 Betaine 0.1 to 3 Brewer's yeast 0.1 to 3 Vitamin complex 0.1 to 5 Mineral complex 0.1 to 5 Vitamin C 0.1 to 5 Vitamin E 0.1 to 5 Arginine 0.1 to 5 Proline 0.1 to 5 Calcium lactate 0.1 to 5 Total amount 100

Experiment 1st feeding growth result Experimental group breed
Number of days exam
Mari number survival
Mari number Survival rate After farming
Weight gain 1
Average weight Daily growth factor Primary One 23 100 88 88.00 - - -

< Example 2>

Experiment 2nd: Sea Edging At 2 nursing homes (1m × 1m × 1m) Puerulus Results of farming the initial 120 for 32 days

Wheat flour, seaweed, fish oil, gluten, arginine, lysine hydrochloride, methionine, cysteine hydrochloride, betaine, lecithin, choline chloride, vitamin C, crab flavor, TGA (transglutaminase), vitamin mixture, mineral mixture, and podzol are commonly used. In Experimental Group 2A, mackerel (domestic refrigerated), fish meal, and krill meal were used, and in Experimental Group 2B, mackerel (domestic refrigerated), squid (seondong), and krill meal were used. Differently, as shown in Table 3, it was prepared in the form of wet feed.

Experimental 2nd feeding feed composition (% by weight) Ingredients Experimental group 2A Experimental group 2B Mackerel 25 to 40 25 to 40 Fish meal 15-25 - squid - 15-25 Krill wheat 5 to 20 5 to 20 Wheat flour 5 to 20 5 to 20 Seaweed 0.1 to 5 0.1 to 5 Fish oil 1 to 5 1 to 5 gluten 0.1 to 5 0.1 to 5 Arginine 0.1 to 3 0.1 to 3 Lysine hydrochloride 0.1 to 3 0.1 to 3 Methionine 0.1 to 3 0.1 to 3 Cysteine hydrochloride 0.1 to 3 0.1 to 3 Betaine 0.1 to 3 0.1 to 3 lecithin 0.1 to 3 0.1 to 3 Choline chloride 0.1 to 5 0.1 to 5 Vitamin C 0.1 to 5 0.1 to 5 Crab flavor 0.1 to 5 0.1 to 5 TGA (transglutaminase) 0.1 to 5 0.1 to 5 Vitamin mix 0.1 to 5 0.1 to 5 Mineral mixture 0.1 to 5 0.1 to 5 Podzol 0.1 to 5 0.1 to 5 Total amount 100 100

Experiment 2nd feeding growth result Round Experimental group breed
Number of days exam
Mari number survival
Mari number Survival rate
(%) After growth
Gross weight (g) 1
Average weight (g) Daily growth factor Secondary 2A 32 60 51 85.00 200 3.92 1.49 2B 60 50 83.33 190 3.80 1.43

The results are shown in detail in Table 4 above. In the above table, the daily growth coefficient was derived by the following equation.

Daily growth coefficient =

사육 후 평균 중량

Average weight after breeding

사육 전 평균 중량

Average weight before breeding

J.GUILLAUME et al. reported that when the level of squid protein increased up to 6% in the breeding experiment of barley shrimp, the growth increased significantly (J. GUILLAUME, E. CRUZ-RICQUE, G. CUZON, A. VAN WORMHOUDT and A. REVOL, Growth factors in Penaeid shrimp feeding, 1989. ADVANCES IN TROPICAL AQUACULTURE), in this experiment, the average weight of one animal in feed 2A with 8% squid and feed 2B without added squid was 3.92 g and 3.80 g, respectively. In the diet containing squid, the weight gain was slightly lower, but the palatability (reactivity to feeding) was observed to be high.

The experimental secondary feed used in the experiment had a relatively similar palatability compared to that of fish farming, but compared to raw materials, growth was insufficient and there were a lot of differences between individuals. In %, it was more than the formula for feeding raw materials compared to young babies, and the amount of feed was insufficient.

In the primary feed of the experiment, compared to the use of 1% glycine, 1% alanine, and 0.5% proline, which are used as attractants, it is judged that the amount of amino acids used as attractants is large, and as a result of excluding all three types of attractants, food It is judged that the amount of feed intake for attractiveness is insufficient.

< Example 3>

Experiment 3rd: Sea Edging At 3 nursing homes (1m × 1m × 1m) Puerulus Results of farming the initial 330 dogs for 35 days

Squid (smelt), fish meal, krill meal, α-potato starch, seaweed, fish oil, gluten, glycine, arginine, lysine hydrochloride, methionine, cysteine hydrochloride, betaine, lecithin, choline chloride, vitamin C, crab flavor, TGA, vitamins Although the mixture, mineral mixture, and podzol were formed in common, the problem of lowering the weight gain compared to the primary feed (the weight of the third feed test was not measured) occurred.In the third experiment, the carbohydrate content was reduced and the amino acid and protein content Increased.

To reduce the carbohydrate source content and increase the protein source content, instead of using wheat flour, α-starch was used.As a result of increasing the amount of fish oil by 1% point from 1% to 2% to increase the lipid source, the secondary The accessibility of the food is much lower than that of the feed, and after 1 hour due to the gelatinization reaction of α-starch, more than 70% of the amount of feed is lost before feeding by the lobster larvae by feeding and washing in the net. The formulation maintenance of the feed was somewhat inferior. In Experimental Group 3A, mackerel (domestic refrigeration) was used, in Experimental Group 3B, mussels (self-cooked in China), and in Experimental Group 3C, the surplus feed of Experimental Group A was cultured for 35 days.

Experiment 3rd feed composition (% by weight) Ingredients Experimental group 3A Experimental group 3B Experimental group 3C Mackerel 15-25 - Surplus feed for experimental group 2A mussel - 15-25 squid 15-25 15-25 Fish meal, krill meal 20 to 40 20 to 40 α-starch 3 to 15 3 to 15 Seaweed 0.1 to 5 0.1 to 5 Fish oil 1 to 5 1 to 5 gluten 0.1 to 4 0.1 to 4 Glycine 0.1 to 3 0.1 to 3 Arginine 0.1 to 3 0.1 to 3 Lysine hydrochloride 0.1 to 3 0.1 to 3 Methionine 0.1 to 3 0.1 to 3 Cysteine hydrochloride 0.1 to 3 0.1 to 3 Betaine 0.1 to 3 0.1 to 3 lecithin 0.1 to 3 0.1 to 3 Choline chloride 0.1 to 5 0.1 to 5 Vitamin C 0.1 to 5 0.1 to 5 Crab flavor 0.1 to 5 0.1 to 5 TGA 0.1 to 5 0.1 to 5 Vitamin mix 0.1 to 5 0.1 to 5 Mineral mixture 0.1 to 5 0.1 to 5 Podzol 0.1 to 5 0.1 to 5 Total amount 100 100

Experiment 3rd feeding growth result Round Experimental group breed
Number of days exam
Mari number survival
Mari number Survival rate (%) After growth
Gross weight (g) 1
Average weight (g) Daily growth factor 3rd 3A 35 110 103 93.64 - - - 3B 110 102 92.73 - - - 3C 110 100 90.91 - - -

The growth rate was observed to be slower than that of raw feed, but it was uniformly progressed in the breeding tank during the molting process. As a result of growing for 10 days, the first peeling process was achieved, and the overall peeling period in the breeding tank was achieved in 3 days.

In experimental group 3A, 103 out of 110 animals survived, in experimental group 3B, 102 out of 110 animals survived, and in experimental group 3C, 100 out of 110 animals survived.

< Example 4>

Experiment 4th: Sea Edging At 3 nursing homes (1m × 1m × 1m) Puerulus Results of farming the initial 300 for 38 days

Krill meal, wheat flour, α-potato starch, seaweed, fish oil, gluten, glycine, arginine, lysine hydrochloride, methionine, cysteine hydrochloride, betaine, lecithin, choline chloride, vitamin C, flavor, TGA, vitamin mixture, mineral mixture, Homophilia was commonly formed, and fish meal was used as the main raw material in experimental group 4A, and mackerel (refrigerated from Vietnam) and fish meal in experimental group 4B, and shellfish (refrigerated from Vietnam) and fish meal were mixed in experimental group 4C.

Experiment 4th feed composition (wt%) Ingredients Experimental group 4A Experimental group 4B Experimental group 4C Fish meal 30 to 50 15-25 15-25 Mackerel - 15-25 - clam - - 15-25 Krill wheat 20 to 40 20 to 40 20 to 40 Wheat flour, α-potato starch 3 to 15 3 to 15 3 to 15 Seaweed 0.1 to 5 0.1 to 5 0.1 to 5 Fish oil 1 to 5 1 to 5 1 to 5 gluten 0.1 to 4 0.1 to 4 0.1 to 4 Glycine 0.1 to 3 0.1 to 3 0.1 to 3 Arginine 0.1 to 3 0.1 to 3 0.1 to 3 Lysine hydrochloride 0.1 to 3 0.1 to 3 0.1 to 3 Methionine 0.1 to 3 0.1 to 3 0.1 to 3 Cysteine hydrochloride 0.1 to 3 0.1 to 3 0.1 to 3 Betaine 0.1 to 3 0.1 to 3 0.1 to 3 lecithin 0.1 to 3 0.1 to 3 0.1 to 3 Choline chloride 0.1 to 5 0.1 to 5 0.1 to 5 Vitamin C 0.1 to 5 0.1 to 5 0.1 to 5 Crab flavor 0.1 to 5 0.1 to 5 0.1 to 5 TGA 0.1 to 5 0.1 to 5 0.1 to 5 Vitamin mix 0.1 to 5 0.1 to 5 0.1 to 5 Mineral mixture 0.1 to 5 0.1 to 5 0.1 to 5 Podzol 0.1 to 5 0.1 to 5 0.1 to 5 Total amount 100 100 100

Experiment 4th feeding growth result Round Experimental group breed
Number of days exam
Mari number survival
Mari number Survival rate (%) After farming
Weight gain (g) 1
Average weight (g) Daily growth factor 4th 4A 38 100 90 90.00 65 0.72 1.13 4B 100 71 71.00 35 0.50 0.87 4C 100 91 91.00 65 0.71 1.13

The daily growth coefficient was calculated in the same manner as in <Example 2>.

When fish with low freshness (mackerel and shellfish, purchased at the Vietnamese fish market), which are felt by the human sense of smell, are used as a raw material food source, access to food is significantly reduced. In addition, when the fish oil was increased from 2% to 3% in order to increase the lipid content, access to food was significantly decreased.

ATP is almost universal as a carrier of chemical energy in metabolic pathways. Richard K. and Zimmer-Faust are particularly abundant in metabolic active tissues such as muscles, but when cells die, ATP is rapidly destroyed into AMP.As the level of ATP decreases, the level of AMP increases. It has been reported to have dropped significantly (Richard K. Zimmer-Faust, ATP: A potential prey attractant evoking carnivory, 1993, LIMONOLOGY AND OCEANOGRAPHY).

Therefore, it was confirmed that the relationship of food inducement according to freshness was confirmed that the habit of food was changed according to the composition ratio of nucleic acids.

In Experimental Group 4A, which focuses on fishmeal as an alternative source of raw material, as a result of experimenting with different concentrations of crab flavor, 0.5% and 1.0%, the preference for food selection increased according to the concentration of crab flavor.

As a result of growth for 38 days, 90 out of 100 animals survived in experimental group 4A, 71 out of 100 survived in experimental group 4B, 91 out of 100 survived in experimental group 4C, and the average weight was 0.72 g, 0.50 g, respectively. It was 0.71 g.

< Example 5>

Experiment 5: Sea Edging In 4 ponds (1m × 1m × 1m) Philosoma Results of farming 300 late-stage animals for 37 days

Squid (stirred), fish meal, krill meal, wheat flour, casein sodium, seaweed, fish oil, gluten, glycine, arginine, lysine hydrochloride, methionine, cysteine hydrochloride, betaine, lecithin, choline chloride, vitamin C, TGA, vitamin mixture, A mineral mixture and podzol were formed in common, and in experimental group 5A, mackerel (domestic frozen) and crab flavor, in experimental group 5B, mussels (domestic refrigeration) and crab flavor, in experimental group 5C, crab (frozen from China), in experimental group 5D, mackerel: Mussels: crabs = 1: 1: 1 was prepared by mixing in a weight ratio.

Experiment 5th feed composition (wt%) Ingredients Experimental group 5A Experimental group 5B Experimental group 5C Experimental group 5D Mackerel 15-25 - - 5 to 10 mussel - 15-25 - 5 to 10 to - - 15-25 5 to 10 squid 15-25 15-25 15-25 15-25 Fish meal, krill meal 20 to 40 20 to 40 20 to 40 20 to 40 Wheat flour 3 to 15 3 to 15 3 to 15 3 to 15 Sodium caseinate 0.1 to 4 0.1 to 4 0.1 to 4 0.1 to 4 Seaweed 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Fish oil 1 to 5 1 to 5 1 to 5 1 to 5 gluten 0.1 to 4 0.1 to 4 0.1 to 4 0.1 to 4 Glycine 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Arginine 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Lysine hydrochloride 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Methionine 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Cysteine hydrochloride 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Betaine 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 lecithin 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Choline chloride 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Vitamin C 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Crab flavor 0.1 to 5 0.1 to 5 - - TGA 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Vitamin mix 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Mineral mixture 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Podzol 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Total amount 100 100 100 100

Experiment 5th Feeding Growth Results Round Experimental group breed
Number of days exam
Mari number survival
Mari number Survival rate (%) Gross weight after growth (g) 1
Average weight (g) Daily growth factor 5th 5A 37 80 71 88.75 55 0.77 1.23 5B 80 72 90.00 70 0.97 1.42 5C 70 62 88.57 50 0.81 1.26 5D 70 67 95.71 55 0.82 1.28

The daily growth coefficient was calculated in the same manner as in <Example 2>.

They were sold in the later stages of Philosoma and put into the breeding tank, but in the process of transforming to puerulus, the process of eating food was not observed.

As a result of stocking 80, 80, 70, and 70 animals in each tank for 37 days, the survival rates were 88.75%, 90.00%, 88.57%, and 95.71%. The survival rate was highest in the tank fed Feed D, but the weight gain was increased. Silver mussels> mixed ≒ crab> mackerel were grown in the order, and it was confirmed that the weight gain was increased due to the increase of the feeding amount according to the food preference in the feed with 1% crab flavor added.

Due to the unique characteristics of the sequence and cluster according to the food intake, the difference in the amount of food intake for each individual occurs.If the deviation of the growing individuals according to the number of molting is severe, the difference in survival rate occurs due to death due to eating each other due to food competition Became.

< Example 6>

Experiment 6th: Sea Edging At 10 homes (1m × 1m) Philosoma Results of farming 1000 of the terminal stages for 42 days

In the latter half of Philosoma, they were distributed from Jongmyo-sa, and fed after acclimatization, and 47 out of 1,000 died before feeding.

In experimental groups 6A and 6E mackerel (domestic frozen mackerel), mussels (domestic refrigeration) in experimental groups 6B and 6F, crabs (frozen made in China) were used in experimental groups 6C and 6G, and mackerel: mussel: crab = 1:1 in experimental groups 6D and 6H. : Mixing at a weight ratio of 1 to prepare a 6th feed (wet feed) as shown in Table 11.

100 animals were received in each tank, and the eight tanks were fed with the feed prepared as shown in Table 11 below, and the remaining two tanks were fed with raw materials (catch and snail) in the farm.

Experiment 6th feed composition (wt%) Ingredients Experimental group 6A Experimental group 6B Experimental group 6C Experimental group 6D Experimental group 6E Experimental group 6F Experimental group 6G Experimental group 6H Mackerel 15-25 - - 5 to 10 15-25 - - 5 to 10 mussel - 15-25 - 5 to 10 - 15-25 - 5 to 10 to - - 15-25 5 to 10 - - 15-25 5 to 10 squid 15-25 15-25 15-25 15-25 15-25 15-25 15-25 15-25 Fish meal, krill meal 20 to 40 20 to 40 20 to 40 20 to 40 20 to 40 20 to 40 20 to 40 20 to 40 Wheat flour 3 to 15 3 to 15 3 to 15 3 to 15 3 to 15 3 to 15 3 to 15 3 to 15 Seaweed 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Fish oil 1 to 5 1 to 5 1 to 5 1 to 5 1 to 5 1 to 5 1 to 5 1 to 5 gluten 0.1 to 4 0.1 to 4 0.1 to 4 0.1 to 4 0.1 to 4 0.1 to 4 0.1 to 4 0.1 to 4 Glycine 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Arginine 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Lysine hydrochloride 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Methionine 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Cysteine hydrochloride 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Betaine 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 lecithin 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Choline chloride 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Vitamin Complex 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Mineral complex 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 TGA 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 - - - - Sodium caseinate - - - - 0.1 to 4 0.1 to 4 0.1 to 4 0.1 to 4 Rotifer culture solution - - - - 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Spirulina - - - - 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Kiwi - - - - 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Sodium alginate - - - - 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Guar gum - - - - 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Crab flavor 0.1 to 5 0.1 to 5 - - 0.1 to 5 0.1 to 5 - - Podzol 0.1 to 5 0.1 to 5 - - 0.1 to 5 0.1 to 5 - - Total amount 100 100 100 100 100 100 100 100

Growth result of the 6th feed feeding Round Experimental group breed
Number of days exam
Mari number survival
Mari number Survival rate
(%) After farming
Weight gain (g) 1
Average weight (g) Daily growth factor 6th 6A 42 96 82 85.42 85 1.04 1.3 6B 95 81 85.26 75 0.93 1.22 6C 96 82 85.42 100 1.22 1.44 6D 93 72 77.42 110 1.53 1.64 6E 97 76 78.35 105 1.38 1.55 6F 94 60 63.83 60 One 1.28 6G 95 88 92.63 135 1.53 1.64 6H 93 71 76.34 100 1.41 1.56 Raw feed 194 157 80.93 320 2.04 1.91

The daily growth coefficient was calculated in the same manner as in <Example 2>.

The survival rates of the mackerel group, mussel group, crab group and mixed group fed with feed were 85.34% 81.48% 71.20% 84.57%, and the average weight was 0.98g, 1.36g, 1.21g, 1.48g.

In the group using mackerel and mussels as raw materials, crab flavor and podzol were added, and in the group using crab as raw materials, the content of raw material (crab) was increased as much as crab flavor and podzol were excluded. The survival rate and average weight were measured in the group with and without the crabhyang and podzol, respectively, and they were 83.42%, 1.17g, 77.84%, and 1.36g. In the group to which crab flavor was added, the difference in weight of the individual was not large, but in the group that increased the raw material of crab by the amount lacking the crab flavor and podzol, the size of the individual was observed to be uneven. It is judged to have declined.

The reason for the highest mortality rate due to the formula due to the difference in the feeding method in the breeding tank is the aggression among individuals to surplus food, which is considered to be consistent with the report that the mortality rate differs depending on the feeding method.

< Example 7>

6th In feed Experimental group Nutritional analysis of 6D and 6G

Based on the standard feed standard analysis method for the 6th general nutrient analysis of the experiment by an accredited certification institution, the weight ratio (w/w %) or g/g/g/g/ It was expressed as 100g fatty acid, and as a result of self-quality inspection, it was verified as shown in the table below.

Analysis of general components of experimental groups 6D and 6G in the 6th feed (w/w %) Inspection items Experimental group 6D Experimental group 6G moisture 44.32 43.40 protein 35.64 35.33 Crude fat 4.12 3.94 Crude fiber 1.11 1.18 Minutes 9.90 9.74

Analysis of amino acid components of experimental groups 6D and 6G in the 6th feed (w/w %) Inspection items Experimental group 6D Experimental group 6G Aspartic acid 2.45 2.54 Threonine 1.15 1.19 Serine 1.18 1.22 Glutamic acid 3.92 4.03 Proline 1.23 1.25 Glycine 1.52 1.60 Alanine 1.45 1.48 Valine 1.37 1.39 Isoleucine 1.14 1.17 Leucine 1.97 2.00 Tyrosine 0.74 0.59 Phenylalanine 1.13 1.16 Histidine 0.58 0.55 Lysine hydrochloride 3.52 3.72 Arginine 3.58 3.75 Cystine 1.12 1.14 Methionine 1.80 1.91 Tritophan 0.27 0.27

Fatty acid composition analysis results of experimental groups 6D and 6G in the 6th feed (g/100g fatty acid) Inspection items Experimental group 6D Experimental group 6G saturation
fatty acid Caprylic acid 0.02 0.03 Capric acid 0.10 0.07 Lauric acid 0.14 0.17 Myristic acid 4.67 5.32 Pentadecanoic acid 0.39 0.37 Palmitic acid 22.48 22.87 Marmaric acid 0.66 1.24 Stearic acid 13.99 7.91 Aragidic acid 0.76 0.73 Unsaturated
fatty acid Myristoleic acid 0.16 0.19 Fendadicenoic acid 0.37 0.41 Palmitoreic acid 4.40 5.19 Margaolitic acid 0.55 0.56 Oleic acid 15.72 15.40 Linoleic acid 4.23 5.03 Linolenic acid 4.53 0.05 Eicosadienoic acid 1.93 2.45 Dihomo gamma-linoleic acid 1.35 1.08 Eicosatrienoic acid 0.50 0.45 Arachidonic acid 0.09 0.10 Eicosapentaenoic acid 8.48 11.28 Docosapentaenoic acid 0.82 0.83 Docosahexaenoic acid 7.77 9.00

Results of inorganic component analysis of experimental groups 6D and 6G in the 6th feed (w/w %) Inspection items Experimental group 6D Experimental group 6G calcium 1.72 1.49 sign 0.79 0.82 potassium 0.18 0.20 magnesium 0.19 0.16 salt 0.77 0.76

< Example 8>

Experiment 7th: Marine Edging At 8 ponds (2m × 2m × 2m) bracket By breeding tank 500 animals in stock

In the first phase (46 days), mussels and crabs were prepared in a ratio of 4:1 in raw materials, and in the second phase (35 days) and the third phase (35 days), mackerel: mussels: crabs = 1: 1: 1 It was prepared by mixing at a weight ratio.

In addition, squid, fish meal, krill meal, wheat flour, sodium caseinate, rotifer culture medium, spirulina, seaweed, fish oil, gluten, glycine, arginine, lysine hydrochloride, methionine, cysteine hydrochloride, betaine, lecithin, choline chloride, vitamin C, kiwi , Sodium alginate, guar gum, vitamin complex, mineral complex, phytase, probiotic, fructooligosaccharide, and monobasic calcium phosphate were added.

The above components were mixed and converted into a ring having a diameter of about 3mm with a moisture content of 32.8%, dried to 13.88%, and stored frozen to feed the lobster larvae. In the late Philosophers, feed was fed to the breeding tank after the transformation to the early puerulus.

Experiment 7th feed composition (wt%) Ingredients 1st Stage 2 and Stage 3 Mackerel - 5 to 10 mussel 10 to 30 5 to 10 to 5 to 15 5 to 10 squid 5 to 15 5 to 15 Fish meal, krill meal 20 to 40 20 to 40 Wheat flour 3 to 15 3 to 15 Sodium caseinate 0.1 to 4 0.1 to 4 Rotifer culture medium 0.1 to 5 0.1 to 5 Spirulina 0.1 to 5 0.1 to 5 Seaweed 0.1 to 5 0.1 to 5 Fish oil 1 to 5 1 to 5 gluten 0.1 to 4 0.1 to 4 Glycine 0.1 to 3 0.1 to 3 Arginine 0.1 to 3 0.1 to 3 Lysine hydrochloride 0.1 to 3 0.1 to 3 Methionine 0.1 to 3 0.1 to 3 Cysteine hydrochloride 0.1 to 3 0.1 to 3 Betaine 0.1 to 3 0.1 to 3 lecithin 0.1 to 3 0.1 to 3 Choline chloride 0.1 to 5 0.1 to 5 Vitamin C 0.1 to 5 0.1 to 5 Kiwi 0.1 to 5 0.1 to 5 Sodium alginate 0.1 to 5 0.1 to 5 Guar gum 0.1 to 5 0.1 to 5 Vitamin complex 0.1 to 5 0.1 to 5 Mineral complex 0.1 to 5 0.1 to 5 Phytase 0.1 to 5 0.1 to 5 Probiotic 0.1 to 5 0.1 to 5 Fructooligosaccharide 0.1 to 5 0.1 to 5 Monobasic calcium phosphate 0.1 to 4 0.1 to 4 Total amount 100 100

Growth results of the 7th feed feeding Item 1st measurement 2nd measurement 3rd measurement 2nd~3rd period measurement result large
individual small
individual sub Total large
individual small
individual sub Total large
individual small
individual sub Total large
individual small
individual sub Total Breeding days 46 35 35 69 Number of test animals 4000 961 420 1381 652 349 1001 961 420 1381 Number of surviving animals 961 420 1381 652 349 1001 598 269 867 598 269 867 Survival rate - - 34.53 67.85 83.10 72.48 91.72 77.08 86.61 62.23 64.05 62.78 Gross weight after growth 1790 380 2170 4530 810 5340 8500 1310 9810 6710 930 7640 Average weight 1.86 0.90 1.57 5.33 7.13 2.38 14.21 4.87 11.31 14.21 4.87 11.31 every day
Growth factor 1.67 1.09 1.52 1.98 1.05 1.67 1.42 1.03 1.42 1.73 1.06 1.57 feed
Conversion efficiency 8.86 5.05 3.58 4.19

The daily growth coefficient was calculated in the same manner as in <Example 2>.

As a result of feeding with dry feed rather than wet feed in the early puerulus, individuals domesticated with the prey had relatively active food intake, but puerulus, which was not tamed for food adaptation, did not adapt to the intake of the feed It is judged to be a food feeding activity mainly focused on plankton in. As a result of feeding twice a day at sunrise and sunset during the first phase (raising for 46 days) after stocking in the late Philosophy, the survival rate was 34.53%, and the average weight of large and small individuals was 1.86 g 0.90 g. There was a contrasting pattern in the size of the liver. The result was consistent with CWThomas et.al's study that the mortality rate was increased due to food competition and nutrient intake of small-sized individuals if the feeding amount was not sufficient (CW Thomas, CG). Carter, BJ Crear, Feed availability and its relationship to survival, growth, dominance and the agonistic behavior of the southern rock lobster, Jasus edwardsii in captivity. 2003. Aquaculture.).

As a result of excessive input of feed from the middle of Puerulus, the amount of food intake at sunrise of the next day decreased significantly when an excess was supplied at dusk. As a result of rearing during the second period (35 days) and the third period (35 days), the amount of food intake and feed conversion rate were different according to the growing season. As shown in the table above, as a result of breeding for 69 days, the survival rates of large and small individuals were 62.23% and 64.05%, which was an average of 62.78%, and daily growth coefficients were 1.73 and 1.06, which was an average of 1.57.

As a result of breeding for 69 days, a result of 4.19 was derived as a result of calculating the feed conversion efficiency of surviving lobster larvae based on the amount of food supplied.

< Example 9>

Experiment 8: Sea Edging In 4 ponds (1m × 1m × 1m) 400 in stock

In the first phase (13 days), in Lobster F1 stage feed, mussels and crabs were mixed in a weight ratio of 2:1 in raw materials, and in the second stage (39 days), mussels in raw materials in Lobster F2 stage feed: crab = 2: 1 was prepared by mixing in a weight ratio of 1: In the third phase (13 days), Lobster F3 stage feed was prepared by mixing herring: mussels: crabs in a weight ratio of 1: 1: 1.

In addition, squid, fish meal, krill meal, wheat flour, sodium caseinate, rotifer culture medium, spirulina, seaweed, fish oil, gluten, glycine, arginine, lysine hydrochloride, methionine, cysteine hydrochloride, betaine, lecithin, choline chloride, vitamin C, kiwi , Sodium alginate, guar gum, vitamin complex, mineral complex, phytase, probiotic, fructooligosaccharide, and calcium monophosphate were added to the composition as shown in Table 20.

Table 19 shows the breeding period of the first period and the breeding method of the second and third periods.

As a result of three days of acclimatization of late Philosophy larvae in a marine general net, eight were killed. 100, 100, 100, 92, 40, and 60 animals were placed in the 8A to 8F breeding tanks.

The 8th feed feeding-breeding tank and feeding method step Breeding tank Feeding method 1st 8A Wet feed supply in the breeding tank of Figure 4a 8B Wet feed supply from general net breeding tanks 8C Raw feed (grabbed and sliced) supplied from general net breeding tanks 8D Mixed wet feed + raw feed (catch and slice) in a general net breeding tank 8E Supply of raw feed (grabbing slices) from the water tank of FIG. 8F Wet feed from the water tank of Figure 4b 2 ~ 3 period 8G Wet feed supply in the breeding tank of Figure 4a 8H Wet feed supply from general net breeding tanks 8I Raw feed (grabbed and sliced) supplied from general net breeding tank 8J Mixed wet feed + raw feed (catch and slice) in a general net breeding tank

Experiment 8th feed composition (wt%) Ingredients Lobster F1 stage feed Lobster F2 stage feed Lobster F3 stage feed herring - - 5 to 10 mussel 10 to 30 10 to 30 5 to 10 to 10 to 30 5 to 15 5 to 10 squid 5 to 15 5 to 10 5 to 10 Fish meal, krill meal 20 to 40 20 to 40 20 to 40 Wheat flour - 15-25 15-25 Rotifer culture solution 0.1 to 5 0.1 to 5 - Spirulina 0.1 to 5 1.5 0.1 to 5 Seaweed 0.1 to 5 0.1 to 5 0.1 to 5 olive oil 0.1 to 5 0.1 to 5 - Fish oil 0 0.1 to 5 0.1 to 5 gluten - - 0.1 to 5 Glycine 0.1 to 3 0.1 to 3 0.1 to 3 Arginine 0.1 to 3 0.1 to 3 0.1 to 3 Lysine hydrochloride 0.1 to 3 0.1 to 3 0.1 to 3 Methionine 0.1 to 3 0.1 to 3 0.1 to 3 Cysteine 0.1 to 3 0.1 to 3 0.1 to 3 Betaine 0.1 to 3 0.1 to 3 0.1 to 3 lecithin 0.1 to 3 0.1 to 3 0.1 to 3 Choline chloride 0.1 to 3 0.1 to 3 0.1 to 3 Vitamin C 0.1 to 3 0.1 to 3 0.1 to 3 Phytase 0.01 to 1 0.01 to 1 0.01 to 1 Probiotic - 0.1 to 3 0.1 to 3 Fructooligosaccharide - 0.1 to 3 0.1 to 3 Sodium alginate 0.1 to 3 0.1 to 3 - Guar gum 0.1 to 3 0.1 to 3 0.1 to 3 vitamin 0.1 to 5 0.1 to 5 0.1 to 5 mineral 0.1 to 5 0.1 to 5 0.1 to 5 Calcium lactate 0.1 to 3 0.1 to 3 0.1 to 3 MCP 0.1 to 3 0.1 to 3 0.1 to 3 system 100 100 100

Groups 8A through 8D tested in the offshore cage farm were affected by waves, but in Groups 8E and 8F tested in the water tank, the vibrations caused by the wind were more severe than the effects of the waves due to the wind, and dissolved oxygen (dissolved oxygen). Oxygen) was constantly supplied by installing an oxygen supply to keep the oxygen constant, but the oxygen supply was not operated due to the interruption of the power supply twice, resulting in a large amount of death due to lack of oxygen supply. There was a loss of population due to.

8th Stage 1 (13 days breeding) Feed feed growth results Feeding
Number of days exam
Mari number survival
Mari number Survival rate After farming
Gross weight 1
Average weight Daily growth coefficient (13 days) 8A 13 100 94 94.00 75 0.80 3.56 8B 13 100 87 87.00 70 0.80 3.58 8C 13 100 91 91.00 65 0.71 3.31 8D 13 92 74 80.43 60 0.81 3.60 8E 13 40 22 55.00 20 0.91 3.88 8F 13 60 17 28.33 20 1.18 4.55

The same food sources were supplied in the breeding tanks 8G and 8H. The stability of the food supply is judged to be an important factor that greatly affects the survival rate and daily growth coefficient values due to differences in the feeding environment.

In the breeding tank 8I, the catches were crushed and supplied uniformly, and in the breeding tank 8J, the mixed feed and raw feed were mixed to provide food. In the breeding tank 8J, the growth rate is judged to be the best because the nutritional demand and feed preservation effects complement each other.

Growth results of the 8th 2nd (49 days breeding) feed feeding Feeding
Number of days exam
Mari number survival
Mari number Survival rate After breeding
Gross weight 1
Average weight Daily growth coefficient (49 days) 8G 49 94 86 91.49 325 3.78 1.28 8H 49 104 52 50.00 135 2.60 0.86 8I 49 113 83 73.45 325 3.92 1.36 8J 49 74 65 87.84 410 6.31 1.87

< Example 10>

Experiment 9: Sea Edging 4 nursing homes (1m × 1m × 1m, 1.5m × 1m × 1m) 510 animals in stock

In the first phase (11 days), in Lobster F1 stage feed, mussels and crabs were mixed in a weight ratio of 2:1 in raw materials, and in the second stage (27 days) and in the third stage (20 days), Lobster F2 stage In feed, it was prepared by mixing mussels: crabs = 2: 1 in the weight ratio of raw materials, and in the fourth phase (29 days), in the Lobster F3 stage feed, herring: mussels: crabs = 1: 1: 1 in the raw materials By mixing, the feed composition for the ninth feed was prepared as shown in Table 23.

510 at the end of Philosoma were placed in the 9A, 9B, 9C, 9D, 9E and 9F tanks, respectively, with 100, 100, and 100. After 110, 50, and 50 were received, they were allowed to acclimate for 3 days. As a result of acclimatization for 3 days, 12 mortality occurred in 9A, 4 mortality in 9B, 4 mortality in 9C, 12 mortality in 9D, 16 mortality in 9E, and 24 mortality in 9F. Occurred. In 9E and 9F, there was no interruption of supply due to continuous supply of oxygen.

Table 24 shows the breeding period of the first period and the breeding method of the second and third periods.

Experiment 9th feed composition (wt%) Ingredients Lobster F1 stage feed Lobster F2 stage feed Lobster F3 stage feed Lobster F3-1 stage feed herring - - 5 to 10 - mussel 10 to 30 10 to 30 5 to 10 - to 10 to 30 5 to 15 5 to 10 - squid 5 to 15 5 to 10 5 to 10 - Fish meal, krill meal 20 to 40 20 to 40 20 to 40 30 to 60 Wheat flour - 15-25 15-25 15-25 Rotifer culture solution 0.1 to 5 0.1 to 5 - - Spirulina 0.1 to 5 1.5 0.1 to 5 0.1 to 5 Seaweed 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 olive oil 0.1 to 5 0.1 to 5 - - Fish oil 0 0.1 to 5 0.1 to 5 0.1 to 5 gluten - - 0.1 to 5 0.1 to 5 Glycine 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Arginine 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Lysine hydrochloride 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Methionine 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Cysteine 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Betaine 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 lecithin 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Choline chloride 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Vitamin C 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 Phytase 0.01 to 1 0.01 to 1 0.01 to 1 0.01 to 1 Probiotic - 0.1 to 3 0.1 to 3 0.1 to 3 Fructooligosaccharide - 0.1 to 3 0.1 to 3 0.1 to 3 Sodium alginate 0.1 to 3 0.1 to 3 - - Guar gum 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 vitamin 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 mineral 0.1 to 5 0.1 to 5 0.1 to 5 0.1 to 5 Calcium lactate 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 MCP 0.1 to 3 0.1 to 3 0.1 to 3 0.1 to 3 system 100 100 100 100

The 9th feed feeding-breeding tank and feeding method step Breeding tank Feeding method 1st 9A Feed F1 stage supply in the breeding tank of Figure 4c 9B Feed F1 stage supply from general net breeding tank 9C Feed F1 stage supply from general net breeding tank 9D Supplied as raw feed (catch and shellfish) 9E Feeding the feed F1 stage in the breeding tank of Figure 4b 9F Feeding the feed F1 stage in the breeding tank of Figure 4b 2nd 9G Feed F2 stage supply in the breeding tank of Figure 4c 9H Feed F2 stage feed from general net breeding tank 9I Feed F2 stage feed from general net breeding tank 9J Supplied as raw feed (catch and shellfish) Stage 3 9G Feed F3 stage supply in the breeding tank of Figure 4c 9H Feed F3 stage feed from general net breeding tank 9I Feed F3-1 stage feed from general net breeding tank 9J Supplied as raw feed (catch and shellfish)

In the breeding tanks 9E and 9F, the survival rate was low due to mortality due to continuous shortage of oxygen supply due to the effect of waves, wind, and temporary suspension of the oxygen supply, as in the 8th phase.

Growth results of feed feeding in the 9th stage 1 (raising for 11 days) Experimental group Feeding
Number of days exam
Mari number survival
Mari number Survival rate After farming
Gross weight 1
Average weight Daily growth coefficient (11 days) 9A 11 88 85 96.59 35.00 0.41 2.53 9B 11 96 94 97.92 30.00 0.32 2.00 9C 11 96 96 100.00 25.00 0.26 1.58 9D 11 98 98 100.00 45.00 0.46 2.80 9E 11 34 11 32.35 5 0.45 2.75 9F 11 26 5 19.23 Not measurable

In the rearing tanks 9E and 9F, where the exchange of seawater was blocked in the early stage of rearing after arrival, the process of peeling and turning to color was slower than 9A to 9D in offshore farms. Breeding tanks 9A to 9D are believed to have grown due to food sources of organic matter and plankton constantly flowing from seawater. However, it is judged that the rapid growth in 9E and 9F in late breeding is higher than the amount of organic matter in seawater due to the accumulated supply of organic matter and nutrient source supplied from the Lobster F1 stage. Therefore, it is judged that the supply of food in the F1 stage is efficient from the time when the feeding activity takes place after acclimatizing for 3 to 5 days in the breeding tank where seawater was exchanged at the beginning of breeding.

As shown in Tables 26 and 27, 9J crushed raw feed (catch and shellfish) has a higher daily growth coefficient than other breeding tanks, but its survival rate is significantly lower.

The 9th 2nd (46 days breeding) feed feed growth results Experimental group Feeding
Number of days exam
Mari number survival
Mari number Survival rate After farming
Gross weight 1
Average weight Daily growth coefficient (27 days) 9G 46 85 83 97.65 105 1.27 0.74 9H 46 94 77 81.91 100 1.30 0.88 9I 46 96 81 84.38 85 1.05 0.82 9J 46 114 89 78.07 190 2.13 1.12

The 9th 3rd (29 days rearing) feed feed growth results Experimental group Feeding
Number of days exam
Mari number survival
Mari number Survival rate After farming
Gross weight 1
Average weight Daily growth coefficient (74 days) 9G 29 83 75 90.36 250 3.33 1.42 9H 29 77 71 92.21 235 3.31 1.38 9I 29 81 70 86.42 230 3.29 1.62 9J 29 89 61 68.54 340 5.57 1.67

Comprehensively, the results of lobster larvae growth according to feeding from the first to the ninth wet feed of the present invention derived from <Example 1> to <Example 10> are summarized in detail in the table below.

Growth results of feed (1st to 9th) Round Experimental group breed
Number of days exam
Mari number survival
Mari number Survival rate
(%) After breeding
Gross weight (g) 1
Average weight (g) Daily growth factor Primary One 23 100 88 88.00 - - - Secondary 2A 32 60 51 85.00 200 3.92 1.49 2B 60 50 83.33 190 3.80 1.43 3rd 3A 35 110 103 93.64 - - - 3B 110 102 92.73 - - - 3C 110 100 90.91 - - - 4th 4A 38 100 90 90.00 65 0.72 1.13 4B 100 71 71.00 35 0.50 0.87 4C 100 91 91.00 65 0.71 1.13 5th 5A 37 80 71 88.75 55 0.77 1.23 5B 80 72 90.00 70 0.97 1.42 5C 70 62 88.57 50 0.81 1.26 5D 70 67 95.71 55 0.82 1.28 6th 6A 42 96 82 85.42 85 1.04 1.30 6B 96 82 85.42 100 1.22 1.44 6C 97 76 78.35 105 1.38 1.55 6D 95 88 92.63 135 1.53 1.64 6E 95 81 85.26 75 0.93 1.22 6F 93 72 77.42 110 1.53 1.64 6G 94 60 63.83 60 1.00 1.28 6H 93 71 76.34 100 1.41 1.56 7th
(1st period) Large object 46 4000 961 - 1790 1.86 1.67 Small objects 420 - 380 0.90 1.09 system 1381 34.53 2170 1.57 1.52 7th
(2nd~3rd period) Large object 69 961 598 62.23 6710 14.21 1.73 Small objects 420 269 64.05 930 4.87 1.06 system 1381 867 62.78 7640 11.31 1.57 8th
(1st period) 8A 13 100 94 94.00 75 0.80 3.56 8B 100 87 87.00 70 0.80 3.58 8C 100 91 91.00 65 0.71 3.31 8D 92 74 80.43 60 0.81 3.60 8E 40 22 55.00 20 0.91 3.88 8F 60 17 28.33 20 1.18 4.55 8th
(2nd~3rd period) 8G 49 94 86 91.49 325 3.78 1.28 8H 104 52 50.00 135 2.60 0.86 8I 113 83 73.45 325 3.92 1.36 8J 74 65 87.84 410 6.31 1.87 9th
(1st period) 9A 11 88 85 96.59 35 0.41 2.54 9B 96 94 97.92 30 0.32 1.99 9C 96 96 100.00 25 0.26 1.59 9D 98 98 100.00 45 0.46 2.79 9E 34 11 32.35 5 0.45 2.77 9F 26 5 19.23 - - - 9th
(2nd~4th period) 9G 74 85 75 88.24 250 3.33 1.01 9H 94 71 75.53 235 3.31 1.09 9I 96 70 72.92 230 3.29 1.15 9J 114 61 53.51 340 5.57 1.35

The size of the breeding tank is 1m × 1m × 1.2m in width × height × height, and a frame is installed so that the feeding source is not easily swept away by the influence of a storm, and feeding about 100 larvae at the end of Philosoma is feeding. It is believed that accessibility and survival rate will be high.

If the results of the experiments from the 1st to the 9th are summed up, they are stocked in a water tank of seawater circulating from the end of the transparent Philosomes and allowed to acclimatize for 3 to 5 days. After the puerulus stage 1 ~ 2 turns dark brown, the stocking initial feed, Lobster F1 stage, is supplied for about 12 to 14 days. In the 3rd and 4th phases of Puerulus, it is considered good to feed the Lobster F2 stage for 45 to 50 days. When the weight of Puerulus is around 1.5g ~ 2.0g, the mortality rate due to formula is high. The mortality rate due to the formula can be reduced by performing screening for individual size. It is desirable to supply F3 stage feed up to 90 days after stocking up to juvenil stage 1 to stage 2 to grow to about 4 to 5 g in weight, and then switch from soft feed to hard feed.

So far, the present invention has been looked at around its preferred embodiments. Those of ordinary skill in the art to which the present invention pertains will be able to understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative point of view rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (7)

Mackerel or herring; mussel; And three kinds of raw materials consisting of crab,
Squid, fish meal, krill meal, wheat flour, seaweed, fish oil, gluten, glycine, arginine, lysine hydrochloride, methionine, cysteine hydrochloride, betaine, lecithin, choline chloride, vitamin C, vitamin complex, mineral complex, sodium caseinate, rotifer culture solution, Spirulina, kiwi, sodium alginate, guar gum, phytase, probiotics, fructo-oligosaccharide and monobasic calcium phosphate are added to form a feed composition for spiny lobster larvae. The method of claim 1,
The three raw materials are mackerel or herring; mussel; And crabs are included in a weight ratio of 1:1:1, and each composition is included in an amount of 5 to 25% by weight based on 100% by weight of the total composition. delete delete The method of claim 1,
The spiny lobster larvae is a composition, characterized in that the larvae from phyllosoma to juvenile larvae stage. delete Claims 1, 2 and 5, comprising the step of feeding the composition of any one of claims to spiny lobster larvae, spiny lobster larvae breeding method.

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