CN103131738B - 用改变乌头酸酶调控元件的细菌发酵生产l-赖氨酸的方法 - Google Patents
用改变乌头酸酶调控元件的细菌发酵生产l-赖氨酸的方法 Download PDFInfo
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- CN103131738B CN103131738B CN201310050196.0A CN201310050196A CN103131738B CN 103131738 B CN103131738 B CN 103131738B CN 201310050196 A CN201310050196 A CN 201310050196A CN 103131738 B CN103131738 B CN 103131738B
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- 238000000034 method Methods 0.000 title claims abstract description 39
- 108010009924 Aconitate hydratase Proteins 0.000 title claims abstract description 22
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- 239000004472 Lysine Substances 0.000 title abstract description 10
- 235000019766 L-Lysine Nutrition 0.000 title abstract description 9
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- 230000009466 transformation Effects 0.000 claims description 54
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- WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 claims description 4
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- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 description 1
- 108700029229 Transcriptional Regulatory Elements Proteins 0.000 description 1
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- 230000023266 generation of precursor metabolites and energy Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 235000018977 lysine Nutrition 0.000 description 1
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- 239000002207 metabolite Substances 0.000 description 1
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- ODBLHEXUDAPZAU-UHFFFAOYSA-N threo-D-isocitric acid Natural products OC(=O)C(O)C(C(O)=O)CC(O)=O ODBLHEXUDAPZAU-UHFFFAOYSA-N 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
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- 230000004102 tricarboxylic acid cycle Effects 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (10)
Priority Applications (10)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310050196.0A CN103131738B (zh) | 2013-02-08 | 2013-02-08 | 用改变乌头酸酶调控元件的细菌发酵生产l-赖氨酸的方法 |
KR1020157024148A KR102127181B1 (ko) | 2013-02-08 | 2014-01-07 | 아코니트산수화 효소 유전자와(또는) 그 조절요소를 개조한 세균 발효를 통한 l-리신 생산방법 |
ES14748825.8T ES2673582T3 (es) | 2013-02-08 | 2014-01-07 | Método de generación de L-lisina por medio de bacterias fermentadoras que poseen un gen de aconitasa y/o un elemento regulador modificado |
JP2015556383A JP6335196B2 (ja) | 2013-02-08 | 2014-01-07 | アコニターゼ遺伝子および/またはその調節エレメントの改変により、l−リジンを生産する方法 |
PCT/CN2014/070228 WO2014121669A1 (zh) | 2013-02-08 | 2014-01-07 | 用改变乌头酸酶基因和/或其调控元件的细菌发酵生产l-赖氨酸的方法 |
EP14748825.8A EP2824186B1 (en) | 2013-02-08 | 2014-01-07 | L-lysine generation method by fermenting bacteria having modified aconitase gene and/or regulatory element |
RU2015134995A RU2792116C2 (ru) | 2013-02-08 | 2014-01-07 | Способ получения L-лизина модифицированием гена аконитазы и/или его регуляторных элементов |
DK14748825.8T DK2824186T3 (en) | 2013-02-08 | 2014-01-07 | L-light generation method using fermentation bacteria with modified aconitic gene and / or regulatory element |
CA2900580A CA2900580C (en) | 2013-02-08 | 2014-01-07 | Method for producing l-lysine by modifying aconitase gene and/or regulatory elements thereof |
US14/384,370 US20160002684A1 (en) | 2013-02-08 | 2014-01-07 | Method for Producing L-Lysine by Modifying Aconitase Gene and/or Regulatory Elements thereof |
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CN201310050196.0A CN103131738B (zh) | 2013-02-08 | 2013-02-08 | 用改变乌头酸酶调控元件的细菌发酵生产l-赖氨酸的方法 |
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CN103131738A CN103131738A (zh) | 2013-06-05 |
CN103131738B true CN103131738B (zh) | 2014-01-15 |
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Families Citing this family (3)
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CN103146772B (zh) * | 2013-02-08 | 2014-06-18 | 宁夏伊品生物科技股份有限公司 | 用乌头酸酶表达弱化和/或酶活性降低的细菌发酵生产l-赖氨酸的方法 |
ES2673582T3 (es) * | 2013-02-08 | 2018-06-22 | Ningxia Eppen Biotech Co. Ltd | Método de generación de L-lisina por medio de bacterias fermentadoras que poseen un gen de aconitasa y/o un elemento regulador modificado |
CN110607313B (zh) * | 2019-09-27 | 2021-06-22 | 内蒙古伊品生物科技有限公司 | 一种高产l-赖氨酸的重组菌株及其构建方法与应用 |
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US8110672B2 (en) * | 2005-04-27 | 2012-02-07 | Massachusetts Institute Of Technology | Promoter engineering and genetic control |
JP2010110217A (ja) * | 2007-02-22 | 2010-05-20 | Ajinomoto Co Inc | L−アミノ酸生産菌及びl−アミノ酸の製造法 |
CN102471790B (zh) * | 2009-07-29 | 2014-10-29 | 味之素株式会社 | 产生l-氨基酸的方法 |
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Denomination of invention: Bacteria fermenting and producing L-lysine method by using changed aconitase to regulate and control unit Effective date of registration: 20200120 Granted publication date: 20140115 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd. Registration number: Y2020640000002 |
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Denomination of invention: Production of L-lysine by bacterial fermentation by changing the regulatory elements of aconitase Effective date of registration: 20210127 Granted publication date: 20140115 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd. Registration number: Y2021640000001 |
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Denomination of invention: Method for producing L-lysine by bacterial fermentation with changing aconitase regulatory element Effective date of registration: 20220323 Granted publication date: 20140115 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd. Registration number: Y2022640000002 |
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Date of cancellation: 20230315 Granted publication date: 20140115 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd.|INNER MONGOLIA EPPEN BIOTECH Co.,Ltd.|Heilongjiang Yipin Biotechnology Co.,Ltd. Registration number: Y2022640000002 |
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Denomination of invention: A method for producing L-lysine through bacterial fermentation by changing the regulatory element of aconitase Granted publication date: 20140115 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd.|INNER MONGOLIA EPPEN BIOTECH Co.,Ltd.|Heilongjiang Yipin Biotechnology Co.,Ltd. Registration number: Y2024640000005 |