CN103121884B - Preparation method of multifunctional bioprotein controlled release fertilizer - Google Patents
Preparation method of multifunctional bioprotein controlled release fertilizer Download PDFInfo
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- CN103121884B CN103121884B CN201310042740.7A CN201310042740A CN103121884B CN 103121884 B CN103121884 B CN 103121884B CN 201310042740 A CN201310042740 A CN 201310042740A CN 103121884 B CN103121884 B CN 103121884B
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 137
- 238000002360 preparation method Methods 0.000 title claims abstract description 51
- 238000013270 controlled release Methods 0.000 title abstract description 20
- 239000003112 inhibitor Substances 0.000 claims abstract description 52
- 239000003895 organic fertilizer Substances 0.000 claims abstract description 35
- WFJIVOKAWHGMBH-UHFFFAOYSA-N 4-hexylbenzene-1,3-diol Chemical compound CCCCCCC1=CC=C(O)C=C1O WFJIVOKAWHGMBH-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 43
- 230000001580 bacterial effect Effects 0.000 claims description 40
- 241000894006 Bacteria Species 0.000 claims description 35
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 29
- 239000004202 carbamide Substances 0.000 claims description 29
- 239000000203 mixture Substances 0.000 claims description 28
- 239000002068 microbial inoculum Substances 0.000 claims description 26
- 229910052757 nitrogen Inorganic materials 0.000 claims description 25
- 238000002156 mixing Methods 0.000 claims description 23
- 229940040145 liniment Drugs 0.000 claims description 21
- 239000000865 liniment Substances 0.000 claims description 21
- 239000000463 material Substances 0.000 claims description 19
- 239000000843 powder Substances 0.000 claims description 19
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 17
- 239000011591 potassium Substances 0.000 claims description 17
- 229910052700 potassium Inorganic materials 0.000 claims description 17
- 239000003921 oil Substances 0.000 claims description 16
- 235000019198 oils Nutrition 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 claims description 14
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 claims description 14
- 235000019837 monoammonium phosphate Nutrition 0.000 claims description 14
- 239000006012 monoammonium phosphate Substances 0.000 claims description 14
- 239000005416 organic matter Substances 0.000 claims description 14
- 230000004151 fermentation Effects 0.000 claims description 13
- 238000000855 fermentation Methods 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 11
- 241000194108 Bacillus licheniformis Species 0.000 claims description 10
- 238000005469 granulation Methods 0.000 claims description 10
- 230000003179 granulation Effects 0.000 claims description 10
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 9
- 230000003716 rejuvenation Effects 0.000 claims description 9
- 240000002791 Brassica napus Species 0.000 claims description 8
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 8
- 239000002374 bone meal Substances 0.000 claims description 8
- 229940036811 bone meal Drugs 0.000 claims description 8
- 210000003746 feather Anatomy 0.000 claims description 8
- 238000009655 industrial fermentation Methods 0.000 claims description 8
- 235000012054 meals Nutrition 0.000 claims description 8
- 230000000813 microbial effect Effects 0.000 claims description 8
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims description 7
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 7
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 7
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims description 7
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims description 7
- 235000012343 cottonseed oil Nutrition 0.000 claims description 7
- DCUJJWWUNKIJPH-UHFFFAOYSA-N nitrapyrin Chemical compound ClC1=CC=CC(C(Cl)(Cl)Cl)=N1 DCUJJWWUNKIJPH-UHFFFAOYSA-N 0.000 claims description 7
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 7
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 7
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 7
- 235000011151 potassium sulphates Nutrition 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 7
- 238000010298 pulverizing process Methods 0.000 claims description 7
- 238000010792 warming Methods 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 244000063299 Bacillus subtilis Species 0.000 claims description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
- 239000010721 machine oil Substances 0.000 claims description 4
- 230000007480 spreading Effects 0.000 claims description 4
- 238000003892 spreading Methods 0.000 claims description 4
- 239000002689 soil Substances 0.000 abstract description 20
- 230000000694 effects Effects 0.000 abstract description 19
- 239000000126 substance Substances 0.000 abstract description 19
- 230000001965 increasing effect Effects 0.000 abstract description 10
- 238000004519 manufacturing process Methods 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 9
- 238000005516 engineering process Methods 0.000 abstract description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 239000011248 coating agent Substances 0.000 abstract description 4
- 238000000576 coating method Methods 0.000 abstract description 4
- 201000010099 disease Diseases 0.000 abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 230000035558 fertility Effects 0.000 abstract description 4
- 230000035764 nutrition Effects 0.000 abstract description 4
- 241000607479 Yersinia pestis Species 0.000 abstract description 2
- 230000000855 fungicidal effect Effects 0.000 abstract 1
- 239000000417 fungicide Substances 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 241000209140 Triticum Species 0.000 description 12
- 235000021307 Triticum Nutrition 0.000 description 12
- 239000001888 Peptone Substances 0.000 description 10
- 108010080698 Peptones Proteins 0.000 description 10
- 244000005700 microbiome Species 0.000 description 10
- 235000019319 peptone Nutrition 0.000 description 10
- 240000008042 Zea mays Species 0.000 description 9
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 9
- 230000009286 beneficial effect Effects 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 9
- 235000015097 nutrients Nutrition 0.000 description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 8
- 239000002131 composite material Substances 0.000 description 8
- 230000001954 sterilising effect Effects 0.000 description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 7
- 239000002585 base Substances 0.000 description 7
- 235000005822 corn Nutrition 0.000 description 7
- 244000068988 Glycine max Species 0.000 description 6
- 235000010469 Glycine max Nutrition 0.000 description 6
- 229910019142 PO4 Inorganic materials 0.000 description 6
- 210000003608 fece Anatomy 0.000 description 6
- 239000002054 inoculum Substances 0.000 description 6
- 235000013336 milk Nutrition 0.000 description 6
- 239000008267 milk Substances 0.000 description 6
- 210000004080 milk Anatomy 0.000 description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 6
- 239000010452 phosphate Substances 0.000 description 6
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 241000220324 Pyrus Species 0.000 description 5
- 241001247821 Ziziphus Species 0.000 description 5
- 235000013339 cereals Nutrition 0.000 description 5
- 239000010871 livestock manure Substances 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 244000179970 Monarda didyma Species 0.000 description 4
- 235000010672 Monarda didyma Nutrition 0.000 description 4
- 235000014443 Pyrus communis Nutrition 0.000 description 4
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 150000001875 compounds Chemical group 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 4
- 229910000397 disodium phosphate Inorganic materials 0.000 description 4
- 235000019800 disodium phosphate Nutrition 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- XHCADAYNFIFUHF-TVKJYDDYSA-N esculin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC(C(=C1)O)=CC2=C1OC(=O)C=C2 XHCADAYNFIFUHF-TVKJYDDYSA-N 0.000 description 4
- 239000011790 ferrous sulphate Substances 0.000 description 4
- 235000003891 ferrous sulphate Nutrition 0.000 description 4
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 4
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 4
- 235000013379 molasses Nutrition 0.000 description 4
- 230000000050 nutritive effect Effects 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 230000000638 stimulation Effects 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- 230000009746 freeze damage Effects 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 239000004323 potassium nitrate Substances 0.000 description 3
- 235000010333 potassium nitrate Nutrition 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 238000009938 salting Methods 0.000 description 3
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 2
- ZPLCXHWYPWVJDL-UHFFFAOYSA-N 4-[(4-hydroxyphenyl)methyl]-1,3-oxazolidin-2-one Chemical compound C1=CC(O)=CC=C1CC1NC(=O)OC1 ZPLCXHWYPWVJDL-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 2
- RDXARWSSOJYNLI-UHFFFAOYSA-N [P].[K] Chemical compound [P].[K] RDXARWSSOJYNLI-UHFFFAOYSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 229940116007 ferrous phosphate Drugs 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000004021 humic acid Substances 0.000 description 2
- 239000010903 husk Substances 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 229910000155 iron(II) phosphate Inorganic materials 0.000 description 2
- SDEKDNPYZOERBP-UHFFFAOYSA-H iron(ii) phosphate Chemical compound [Fe+2].[Fe+2].[Fe+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O SDEKDNPYZOERBP-UHFFFAOYSA-H 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 235000009973 maize Nutrition 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 238000009335 monocropping Methods 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 238000007747 plating Methods 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000609240 Ambelania acida Species 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000194107 Bacillus megaterium Species 0.000 description 1
- 241000195940 Bryophyta Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 244000241235 Citrullus lanatus Species 0.000 description 1
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
- 239000005696 Diammonium phosphate Substances 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- WZLMXYBCAZZIRQ-UHFFFAOYSA-N [N].[P].[K] Chemical compound [N].[P].[K] WZLMXYBCAZZIRQ-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001458 anti-acid effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000010905 bagasse Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 230000000536 complexating effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 229910000388 diammonium phosphate Inorganic materials 0.000 description 1
- 235000019838 diammonium phosphate Nutrition 0.000 description 1
- 235000021245 dietary protein Nutrition 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002631 hypothermal effect Effects 0.000 description 1
- 238000009863 impact test Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000011499 joint compound Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000003077 lignite Substances 0.000 description 1
- 150000002826 nitrites Chemical class 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000021749 root development Effects 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000003971 tillage Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention belongs to preparation of a fertilizer and particularly relates to a preparation method of a multifunctional bioprotein controlled release fertilizer. The preparation method comprises the following process steps of: granulating an organic fertilizer, preparing a fungicide, coating mycoderm, coating a nitrification inhibitor, preparing the bioprotein controlled release fertilizer and the like. The preparation method disclosed by the invention solves the prominent problems that when a biological fertilizer in the prior art and a chemical fertilizer are applied at the same time, strains are easily killed by hyperosmosis generated by the chemical fertilizer, soil are hardened, the fertility is reduced, crop diseases and pest are serious, agricultural output and quality are reduced and the like. The preparation method disclosed by the invention has an advanced production technology and selects high-grade raw materials; the nutrition is comprehensive and the content is high; and the fertilizer has many functions and has the obvious effects of reducing soil activity, fertilizing soil fertility, resisting diseases, increasing the production and improving the quality.
Description
Technical field
The invention belongs to the preparation of fertilizer, refer to especially a kind of preparation method of multifunctional bio albumen release and release control fertilizer.
Background technology
The healthy and strong growth needs fertile soil of crop does basis, and fertile soil not only should have various big or middle, micro-inorganic nutrients and the organotrophies such as plentiful nitrogen phosphorus potassium, also should have the beneficial microbe colony of some amount.As not enough or shortage need to apply by enough, just can make its target output be achieved.
Conventional composite (blending) fertilizer, because only containing nitrogen, phosphorus, potassium macroelement.For many years, in arviculture, chemical fertilizer consumption constantly increases, and the consumption of organic fertilizer is fewer and feweri, to plant the phenomenon of not increasing soil fertility and give prominence to, the excessive inorganic fertilizer of using not only causes sharply successively decreasing of fertilizer volume increase return, improving constantly of planting cost, can serve negative impact to agricultural belt.First be to cause soil soda acid; Next is to cause soil compaction, and fertility declines, and affects the existence of soil microorganisms, and quantity reduces; The 3rd is to weaken crops throughput, is mainly manifested in easily lodging and generation disease and pest of farm crop, and disease-resistant, cold-resistant, drought-resistant ability weakens; The 4th can cause quality of agricultural product to decline, to HUMAN HEALTH constitute a serious threat (the edible agricultural-food that contain excessive nitrite salt of human body can cause poisoning, dead and bring out malignant tumour).Long agricultural tillage that this robs formula certainly will be advocated with country in a word full nutrition, multi-functional, improve chemical fertilizer utilization ratio, ensure agricultural product security, safeguard that the sustainable and agriculture sound development of soil disagrees.
For meeting the healthy and strong growth of crop, not merely need chemical fertilizer, also to depend on organic fertilizer and beneficial microorganism cooperation and cooperation, reduction soil activation.Therefore, organic fertilizer, microbial fertilizer arise at the historic moment, and single result of use and function all show excellence, but peasant is in agriculture production reality, because the bacterial classification in most in the market bio-fertilizers cannot be resisted killing of " high osmotic pressure " that chemical fertilizer produces in mineral manure, can only emphasize in the use to avoid to use with chemical fertilizer (this had both increased fertilising cost, had also changed common fertilising custom) simultaneously.Therefore, microbial fertilizer application quantity but only accounts for 1% left and right of fertilizer amount in China, well below developed country's levels such as U.S.'s (9%).So, on market, composite organic-inorganic fertilizer starts to occur, also there is minority manufacturer production to go out composite microbiological fertilizer, but there is following problem, the one, inorganic nutrients content low (NPK total content is no more than 35%), also need in actual applications increasing to join part mineral manure and could meet the healthy and strong needs of growing of crop, the 2nd, organic matter material class is compared with low and probiotics content is few, organic matter material in the composite microbiological fertilizer of selling in the market has humic acid powder (containing humic acid), industrial fermentation by product (vinasse, vinegar grain, bacterium slag, Gourmet Powder Factory's tankage, containing amino acid), cake fertilizer (containing organonitrogen), animal dung and stalk (rice husk, wheat husk, bagasse, plant ash, sawdust) pile fermentation material and chitosan, weathered coal, brown coal, vermiculite, mud, the natural resourcess such as the peat composed of rotten mosses, the few content of the beneficial organism bacterial classification class of allocating into is low, and for example microbial bacteria has vinelandii (root nodule bacterium), phosphorus-solubilizing bacteria (bacillus megaterium, Bacillus licheniformis, subtilis), silicate bacteria is potassium solubilizing bacteria (colloid bacillus cereus).Only having of having is wherein a kind of, function singleness; Also 2-3 the bacterial strain only of composite bacteria, function is also sufficiently complete.Its effective active bacterial content (2,000 ten thousand/g) in, and the high temperature resistance having, resist drying, antiacid alkali, anti-salinity ability are strong not, mortality in fertiliser production or preservation process, survival rate is low, after using, is difficult to get a desired effect.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of multifunctional bio albumen release and release control fertilizer, improve to reach fertilizer inorganic nutrients content, promote organotrophy grade, increase the object of effective active bacterial content.
Overall technology design of the present invention is:
The preparation method of multifunctional bio albumen release and release control fertilizer, comprises following processing step:
A, organic fertilizer granulation
Be rapeseed cake according to ratio of quality and the number of copies: the ratio of feather meal: bone meal: auxiliary material=25-35:25-35:18-22:18-22, by granulation shape fertilizer after above-mentioned raw materials mixture, pulverizing, auxiliary material is selected a kind of or its mixture in cottonseed cake or stalk;
B, microbial inoculum preparation
Accessing separately fermention medium by bacterial classification rejuvenation respectively, after spreading cultivation ferments, to after the bacterium liquid expansion drying after fermentation, make microbial inoculum, described bacterial classification is selected Bacillus licheniformis (Bacillus licheniformis) CCTCCNO:M2013021, subtilis (Bacillus subtilis) CCTCCNO:M2013019 and bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CCTCCNO:M2013020; Above-mentioned bacterial classification is submitted the center preservation of Chinese Typical Representative culture collection on January 16th, 2013, and depositary institution is referred to as CCTCC, and the address of depositary institution is positioned at Wuhan City, Hubei Province Wuhan University.
C, mycoderm dressing
The microbial inoculum mixture of making in step B is made to mixing dry bacterial powder, be mixing dry bacterial powder according to ratio of quality and the number of copies: the ratio of carboxymethyl cellulose: water=2.25-5:0.03-0.05:1.5-3 is made liniment, it is liniment according to ratio of quality and the number of copies: the ratio of particulate state fertilizer=3.78-8.05:470-1000, liniment is coated on to the particulate state fertilizer surface of preparing in steps A, after being dried, makes biological organic fertilizer under 40 DEG C of-50 DEG C of conditions;
D, nitrification inhibitor dressing
It is nitrification inhibitor in ratio of quality and the number of copies: the ratio of No. 68 machinery oil=0.8-1:2.6-3, nitrification inhibitor is poured in No. 68 machinery oil, be warming up to 68 DEG C-72.5 DEG C, being stirred to the chloro-6-trimethylpyridine of 2-dissolves, be under 68 DEG C of-72.5 DEG C of conditions in temperature, be nitrification inhibitor machine oil solution according to ratio of quality and the number of copies: the ratio of urea=0.98-1:980-1000, the nitrification inhibitor after dissolving is sprayed on to urea surface, nitrification inhibitor is selected N-serve;
The preparation of E, bioprotein release and release control fertilizer
It is nitrogen element according to biological bacteria 0.2 hundred million/g and ratio of quality and the number of copies: the ratio of phosphoric: potassium element: organic matter=13-28:4-20:5-17:15-20, according to different requirements, granular organic fertilizer mixture prepared by biological organic fertilizer prepared by step C, nitrification inhibitor coated urea, monoammonium phosphate, Repone K or potassium sulfate prepared by step D and steps A is even, makes bioprotein release and release control fertilizer.
For meeting the interpolation of the elements such as nitrogen, phosphorus, potassium and obtaining, can it is evident that, its compound form can also adopt the chemical fertilizer kind that comprises that diammonium phosphate, saltpetre etc. are common, does not all depart from essence of the present invention.
Concrete technical characterstic of the present invention and processing parameter also have:
In the preparation of microbial inoculum, can adopt prior art to realize easily because the rejuvenation of bacterial classification spreads cultivation etc., the progression spreading cultivation depends primarily on the output of fermentation.Wherein common technology realizes means and is, described step B microbial inoculum preparation comprises that the rejuvenation of bacterial classification spreads cultivation and makes preparation, the industrial fermentation of shake-flask seed, seed liquor.
In the preparation of described step B microbial inoculum, the rejuvenation of bacterial classification spreads cultivation, and to make shake-flask seed processing step as follows:
The preparation of B1, bacterial classification plane
The proportioning of plating medium is as follows:
Peptone 10g, extractum carnis 10g, sodium-chlor 50g, agar 18g, potassium primary phosphate 0.09g, 0.2% bromothymol blue liquid 20ml, distilled water 1000ml;
First extractum carnis, peptone, sodium-chlor are dissolved in distilled water, adjust pH value=7.5, add agar, heating for dissolving, filtration; Add indicator, by sterilizing after 10 flasks of substratum packing, each flask adds sucrose 1g, and dissolve and be cooled to 50 DEG C, pour plate, inoculum size is 1 ring, under 28 DEG C of conditions, cultivates 3-5 days, and each flat board is divided into 12 parts;
The preparation of B2, shake-flask seed
The proportioning of shake-flask seed substratum is as follows:
Peptone 5g, saltpetre 2.5g, ferrous phosphate 1g, magnesium sulfate 0.5g, the soya-bean milk 1000ml that mass percent is 1:40;
The quality such as above-mentioned shake-flask seed substratum are divided into 12 parts, and after sterilizing, access prepared bacterial classification plane in step B1 1 part, under 28 DEG C of conditions, shaking table is cultivated 36 hours, and pH=7.5, obtains shake-flask seed.
In described step B microbial inoculum preparation, the step of preparation process of seed liquor is as follows:
The Medium Proportion of B3, preparation seed liquor is as follows:
Peptone 350g, molasses 700g, potassium primary phosphate 45g, sodium hydrogen phosphate 42g, ferrous sulfate 5g, Vitamin C2 35g, the soya-bean milk 300kg that mass percent is 1:100, the corn steep liquor 50kg that mass percent is 1:100;
By cooling after above-mentioned medium sterilization, the shake-flask seed of making in the inoculum size that is 5% according to mass percent access step B2, fermentation is stirred in sealing oxygen supply, adopt hydrochloric acid or sodium hydroxide to adjust fermented liquid pH=7.5, after 24 hours, make seed liquor, leavening temperature is 29.5 DEG C-37.2 DEG C, and oxygen-supplying amount is 1:1, and mixing speed is 150 revs/min.
Industrial fermentation process step in described step B microbial inoculum preparation is as follows:
The Medium Proportion of B4, industrial fermentation is as follows:
Peptone 3.5g, molasses 7kg, potassium primary phosphate 910g, Sodium phosphate dibasic 840g, ferrous sulfate 35g, Vitamin C2 350g, the soya-bean milk 3000kg that mass percent is 1:100, the corn steep liquor 500kg that mass percent is 1:100;
By cooling after above-mentioned medium sterilization, the seed liquor of making in the inoculum size that is 10% according to mass percent access step B3, fermentation is stirred in sealing oxygen supply, adopt hydrochloric acid or sodium hydroxide to adjust fermented liquid pH=7.5, after 24 hours to bacterium number be the complete bacterium liquid that obtains of 10,000,000,000/ml fermentation, leavening temperature is 29.5 DEG C-37.2 DEG C, and oxygen-supplying amount is 1:1, and mixing speed is 150 revs/min.
In described step C, mixing dry bacterial powder is bacillus licheniformis agent according to ratio of quality and the number of copies: bacillus subtilis microbial agent: the ratio preparation of bacillus amyloliquefaciens microbial inoculum=2:1:1.
After dry in described step C, making mass percent moisture in biological organic fertilizer is 5%-8%.
For realizing the balance of inorganic nutrients, organotrophy and microorganism beneficial bacterium in bioprotein release and release control fertilizer, preferred technical scheme is, inorganic nitrogen in the bioprotein release and release control fertilizer that described step e is made: the ratio of quality and the number of copies of organonitrogen is 20-40:60-80, organonitrogen derives from fertilizer and biological organic fertilizer prepared by urea and steps A, C, and inorganic nitrogen source is in monoammonium phosphate.
Applicant adopts the prepared multifunctional bio albumen release and release control fertilizer of the present invention, and (commodity root power by name is many, lower same.) carry out a large amount of field experiments, experimental result is as follows:
Experiment 1
In Tong Anzhuan village, Liu Zao town of Dingzhou county, Baoding Xu Lei (telephone number: 15133264028) 7.5 mu of plantation wheats, wheat breed is stone new 733, every mu, base fertilizer is used multifunctional bio albumen Controlled Release Fertilizer 40kg prepared by the present invention, wherein stay mu to use other compound manure of equal value for contrast, other management is all identical, surveys on June 6th, 2012 and produces result:
Liu Zao town, Dingzhou county, Baoding Tong Anzhuan village Xu Lei wheat is surveyed and is produced result
Can find out from upper table result: use many bioproteins of root power Controlled Release Fertilizer and do base fertilizer mu spike number, grain number per spike, per mu yield and increase respectively 2.86 ten thousand fringes, 2.65 grains, 69.38Kg, stimulation ratio 15.31% with being worth other fertilizer.
Experiment 2 (effect of increasing production is obvious):
In Zhao naval of Xi Yang village, town of Xingtai City Neiqiu County government's bank (telephone number: 15075995521) 6 mu of plantation Jimai 22 wheats, wherein every mu, 3 mu of base fertilizer is used many bioproteins of root power Controlled Release Fertilizer 40Kg, wherein use other compound manure of equal value for contrast for 3 mu, other management is all identical, surveys on May 28th, 2012 and produces result:
Zhao naval of Xi Yang village, town of Xingtai City Neiqiu County government's bank wheat is surveyed and is produced result
Can find out from upper table result: use many bioproteins of root power Controlled Release Fertilizer and do base fertilizer mu spike number, grain number per spike, per mu yield and increase respectively 5.47 ten thousand fringes, 0.7 grain, 68.85Kg, stimulation ratio 17.38% with being worth other fertilizer.
Experiment 3 (enhancing resistance: cold-resistant):
Large block of wood village, the Korla City Shang Hu of Xinjiang autonomous region town, Mo Yidun. sub-raw (Uygurs) phone: 15001577664.
Target crop: 15 years growth bergamot pears, area: 50 mu.
Time: in September, 2011-2012 year May
Demonstration fertilizer: many bioproteins of root power Controlled Release Fertilizer uses autumn, every strain 2.5Kg.
Effect explanation: bergamot pear was September 20, and trench digging uses, water in full field subsequently.Meet with continuous low temperature 22 days winter in 2011, subzero 29 degree of extreme temperature hypothermic phase, continue one week.Cause Kurle region big area bergamot pear freeze injury to occur.At the beginning of 5 months, observe the contrast field less than 30% of blooming, fruit-setting rate less than 25%, branch freeze injury incidence is more than 60%.Model field freeze injury branch is less than 2%, and fruit-setting rate is normal, and needs secondary fruit thinning.This effect obtains the local compatriot of Uygurs accreditation, and Kuerle delicious pear institute and bergamot pear association have given to assert and high evaluation to demonstration effect simultaneously.
Experiment 4 (supporting short long, the increasing yield and improving quality of root):
How the He Chen village, Cang County, Hebei province kindly helps secure the success of (15833175808)
Target crop: Golden jujube, 3000 mu of areas (the organic little jujube large-scale production of country demonstration area)
Time: in June, 2011-2011 year October
Demonstration fertilizer: many bioproteins of root power Controlled Release Fertilizer
Effect explanation: demonstration area adopts government procurement behavior, unified technology, unified management, unified rich water, before and after June 20 and October 5, every strain is used many bioproteins of root power Controlled Release Fertilizer 2Kg respectively.On year-on-year basis the white ripe phase of other jujube gardens observe the local underproduction serious split jujube, the jujube phenomenon that falls reduce 84%, leaf look dark green is, plump, individual plant root system increases by 38 centimetres on a year-on-year basis, capillary root increase by 13.4.3 groups of model field mu volume increase 74.3Kg on year-on-year basis after results.
Experiment 5 (early maturing and high yields):
Pingyuan County, the Shandong Province king township Zhang Shujing (13869288315) that divines by casting lots
Target crop: canopy early mature watermelon in 4 meters, area: 6 mu
Time: the 3-6 month in 2012
Demonstration fertilizer: many bioproteins of root power Controlled Release Fertilizer
Effect explanation: Wei Laogua district, this area, continuous cropping continuous cropping is general.Every mu of application of organic fertilizers of model field 2 sides, the many 40Kg of root power, contrast every mu, field application of organic fertilizers 4 sides, other are with content composite fertilizer 50Kg.Seedling stage is sent out seedling early in the performance of each stage in the time of infertility, 30 centimetres of climing length, contrast on year-on-year basis field early 5 days, the ripe listing of model field contrasts early 11 days (home town is planted the first family with form) of field on year-on-year basis, mu volume increase 247Kg, cost-saving 185 yuan of COMPREHENSIVE CALCULATING demonstration field, 0.14 yuan of price increase per jin, by 7000 jin of this areas mean level (ML), mu increases income 1165 yuan.
Experiment 6 (support root strong sprout, cost-saving synergistic):
Bei Miao village of Linyi County of Shandong Province rather passes increasing (13791319789)
Target crop: corn (moral agriculture 958), area: 12 mu
Time: in September, 2011
Demonstration fertilizer: many bioproteins of root power Controlled Release Fertilizer
Effect explanation: 12 mu of model fields, wherein 6 mu use the many every mu of 33Kg of root power, and the later stage exempts to topdress.Other 6 mu adopt conventional corn composite fertilizer special mu base fertilizer 25Kg, and the later stage imposes urea 23Kg.119.6 yuan of demonstration field costs, contrast field cost: 120 yuan.Contrast well developed root system mid-term, aerial root increases by 4 on year-on-year basis, without lodging phenomenon.After receiving, survey and produce model field per mu yield 649Kg, 589Kg is produced in contrast field, mu volume increase 60Kg.Save artificial 13, increase income 112 yuan.Effect is remarkable.
Experiment 7 (anti-saline and alkaline high yield):
Chang Guo village of Chang Guo township, Huanghua City, Hebei province platinum friend (13483776654)
Target crop: saltings wheat (Ji wheat 32) 4 families amount to 23 mu
Time: on May 20th, 2012
Effect explanation: home town is commonly moderate saltings, P in soil H value 7,8-8, between 5, causes wheat to emerge of poor quality, the deficiency of tillering, later stage early ageing is obvious, and thousand seed weight maintains breediness 70% left and right all the time.Per mu yield over the years is many at 220-300Kg.For alkaline land improving requirement, introduce many bioproteins of root power Controlled Release Fertilizer, obtain at corn on the basis of good result, select 4 families to amount to 23 mu of wheats and contrast as the common same valency of model field and other composite fertilizer.The operative technique such as sowing, manage, water is equal to.Period of seedling establishment performance is sent out seedling early, turns green faster than contrasting 3 days, field, after watering, without saline and alkaline vestige, surveys native model field pH value on April 12 and reduces 0.5-0.86.May 20 was surveyed product on the spot by Wheat In Hebei Province expert group, and mu spike number increases by 120,000, and single fringe granulating increases 2-3.Model field average yield per mu 423Kg.The improving yield of wheat record of wound saltings, Huanghua.
Experiment 8 (the production-increasing function impact test of different components on spring maize in bioprotein Controlled Release Fertilizer):
| Process numbering | Fertilizer variety | Substance |
| 1 | Bioprotein Controlled Release Fertilizer (28-4-8) | Wherein protein and biological bacteria each 50% |
| 2 | Conventional Chemical Mixed Fertilizer (28-4-8) | Add 50% protein |
| 3 | Conventional Chemical Mixed Fertilizer (28-4-8) | Add 50% biological bacteria |
| 4 | Conventional Chemical Mixed Fertilizer (28-4-8) | Add 50% organic matter |
| 5 | Conventional Chemical Mixed Fertilizer (28-4-8) | 0 |
| 6 | Ck (the blank district of not applying fertilizer) | 0 |
Process all by base manure 40Kg/666.7m upper table 1～5
2, disposable applying.
Can find out from upper watch test result: be up to 736.1 kilograms with the output of bioprotein Controlled Release Fertilizer, use the conventional Chemical Mixed Fertilizer volume increase 19.42% of same inorganic nutrients, secondly add the more conventional Chemical Mixed Fertilizer volume increase 14.67% of protein for conventional Chemical Mixed Fertilizer, and conventional Chemical Mixed Fertilizer add the stimulation ratio of biological bacteria come add organic after, drop on the 4th.The conventional Chemical Mixed Fertilizer of tracing it to its cause adds biological bacteria in biological bacteria processing and has not only lacked good food---protein, and inorganic fertilizer has a strong impact on the performance of its effect.And biological bacteria, protein and inorganic nutrients rational Match in bioprotein Controlled Release Fertilizer, three kinds of nutrition-concerted effects, effect of increasing production is the most outstanding.
Also explanation simultaneously, used protein to be added in fertilizer as organic matter, and the effect of increasing production of spring maize is much better than to use common organic matter, adds the more common equal size Bulk Blending Fertilizer stimulation ratio of protein than adding organic raising 109.57%.
The substantive distinguishing features that the present invention is obtained and significant technical progress are:
1, the full content of nutrition is high: bioprotein Controlled Release Fertilizer nutrient content inorganic nutritive element prepared by employing the present invention is between 30-48, organic between 15-20, more than the active bacterium 2,000 ten thousand/g of beneficial microorganism, wherein, in the nondecreasing situation of the active bacterium of beneficial microorganism, can effectively realize nitrogen, phosphorus, potassium and organic balance and supply with.Can not only meet separately to plant growth and provide outside required NPK and various trace element, can also provide organic and beneficial microorganism activity bacterium for crop.And need to use separately according to Different Crop base fertilizer fertilising, needn't increase again and join other inorganic nutrients and can meet crop to inorganic organic beneficial microorganism nutritional need.
2, slowly-releasing is efficient: the bioprotein Controlled Release Fertilizer that adopts the present invention to prepare, organotrophy possesses biological slow-released function after by biological bacteria coating, wherein nitrogen nutrition reaches controlled-release effect after nitrification inhibitor coating, and in September 2 in 2011 Nikkei Hebei Province Agriculture And Forestry Sciences Agricultural Resources And Environment Institute water seaoning to bioprotein slow-release fertilizer nitrogenous fertilizer release rate assay conclusion: initial stage solubility rate is 9.92%, in compound national standard, control the requirement of release and release control fertilizer initial stage nutrient release rate≤15%, in the requirement that continues also to meet in test 28 days accumulation nutrient release rate≤75%. and in process of production, after dissolving, part inorganic nutritive element adsorbed complexing together by organic, form organic NPK, entering soil is difficult for being run off and fixing, chemical fertilizer utilization ratio can improve 10%-30%, the sustainable 3-9 of fertilizer efficiency month.
3, organic of high grade, remarkably productive: organic matter material used in the present invention is the feed grade materials such as rapeseed cake, feather meal, bone meal, wherein also contain the high-grade nutritive ingredient such as animal proteinum and Calcium of intensifiens, how through, many crop demonstration application effects are obviously better than other fertilizer.
4, molten phosphorus potassium decomposing, fertile soil loosens the soil: fertilizer enters after soil, microorganism is amount reproduction under the assistance of organic matter, inorganic nutritive element, moisture, temperature, reduce the living space of harmful microorganism colony, thereby increase the quantity of soil beneficial microorganism bacterium, microbial bacteria produces a large amount of organic acids and can be partly dissolved to discharge for crop and again absorb being deposited on for many years phosphorus potassium element in soil, and after life-time service, soil will become more and more loose and fertile.
5, strong plantlets and rootage, upgrading volume increase: after microbial bacteria is bred in soil, produce a large amount of plant hormone and organic acid, stimulate root growth and development, strengthen the rate of photosynthisis of farm crop, plant growth is well established and vigorously developing, can effectively improve the pol of crop and fruit, in reduction crop product, the content of nitrate and other objectionable impurities, improves quality, and farm crop can be increased production 10%-30%.
Bacillus licheniformis (Bacillus licheniformis) CCTCCNO:M2013021, subtilis (Bacillus subtilis) CCTCCNO:M2013019 and bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CCTCCNO:M2013020; Above-mentioned bacterial classification is submitted the center preservation of Chinese Typical Representative culture collection on January 16th, 2013, and depositary institution is referred to as CCTCC.
Embodiment
Below in conjunction with embodiment, the present invention is described further; but not as a limitation of the invention; the content that protection scope of the present invention is recorded with claim is as the criterion, and any equivalence techniques means of making according to specification sheets are replaced, and all do not depart from protection scope of the present invention.
Embodiment 1
The preparation method of multifunctional bio albumen release and release control fertilizer, comprises following processing step:
A, organic fertilizer granulation
Be rapeseed cake according to ratio of quality and the number of copies: the ratio of feather meal: bone meal: auxiliary material=25:25:18:18, by granulation shape fertilizer after above-mentioned raw materials mixture, pulverizing, auxiliary material is selected a kind of or its mixture in cottonseed cake or stalk;
B, microbial inoculum preparation
Accessing separately fermention medium by bacterial classification rejuvenation respectively, after spreading cultivation ferments, to after the bacterium liquid expansion drying after fermentation, make microbial inoculum, described bacterial classification is selected Bacillus licheniformis CCTCCNO:M2013021, subtilis CCTCCNO:M2013019 and bacillus amyloliquefaciens CCTCCNO:M2013020;
C, mycoderm dressing
The microbial inoculum mixture of making in step B is made to mixing dry bacterial powder, be mixing dry bacterial powder according to ratio of quality and the number of copies: the ratio of carboxymethyl cellulose: water=2.25-5:0.03-0.05:1.5-3 is made liniment, it is liniment according to ratio of quality and the number of copies: the ratio of particulate state fertilizer=3.78:470, liniment is coated on to the particulate state fertilizer surface of preparing in steps A, after being dried, makes biological organic fertilizer under 40 DEG C of-50 DEG C of conditions;
D, nitrification inhibitor dressing
It is nitrification inhibitor in ratio of quality and the number of copies: the ratio of No. 68 machinery oil=0.8:2.6, nitrification inhibitor is poured in No. 68 machinery oil, be warming up to 68 DEG C, being stirred to the chloro-6-trimethylpyridine of 2-dissolves, be under 68 DEG C of conditions in temperature, be nitrification inhibitor machine oil solution according to ratio of quality and the number of copies: the ratio of urea=0.98:980, the nitrification inhibitor after dissolving is sprayed on to urea surface, nitrification inhibitor is selected N-serve;
The preparation of E, bioprotein release and release control fertilizer
It is nitrogen element according to biological bacteria 0.2 hundred million/g and ratio of quality and the number of copies: the ratio of phosphoric: potassium element: organic matter=18:16:6:19, biological organic fertilizer prepared by step C, nitrification inhibitor coated urea prepared by step D and, the granular organic fertilizer computer mixture prepared of monoammonium phosphate, Repone K or potassium sulfate and steps A is even, makes bioprotein release and release control fertilizer.
The preparation of described step B microbial inoculum comprises that the rejuvenation of bacterial classification spreads cultivation and makes preparation, the industrial fermentation of shake-flask seed, seed liquor.
In the preparation of described step B microbial inoculum, the rejuvenation of bacterial classification expands that to be mixed with shake-flask seed processing step as follows:
The preparation of B1, bacterial classification plane
The proportioning of plating medium is as follows:
Peptone 10g, extractum carnis 10g, sodium-chlor 50g, agar 18g, potassium primary phosphate 0.09g, 0.2% bromothymol blue liquid 20ml, distilled water 1000ml;
First extractum carnis, peptone, sodium-chlor are dissolved in distilled water, adjust pH value=7.5, add agar, heating for dissolving, filtration; Add indicator, by sterilizing after 10 flasks of substratum packing, each flask adds sucrose 1g, and dissolve and be cooled to 50 DEG C, pour plate, inoculum size is 1 ring, under 28 DEG C of conditions, cultivates 3-5 days, and each flat board is divided into 12 parts;
The preparation of B2, shake-flask seed
The proportioning of shake-flask seed substratum is as follows:
Peptone 5g, saltpetre 2.5g, ferrous phosphate 1g, magnesium sulfate 0.5g, the soya-bean milk 1000ml that mass percent is 1:40;
The quality such as above-mentioned shake-flask seed substratum are divided into 12 parts, and after sterilizing, access prepared bacterial classification plane in step B1 1 part, under 28 DEG C of conditions, shaking table is cultivated 36 hours, and pH=7.5, obtains shake-flask seed.
In described step B microbial inoculum preparation, the step of preparation process of seed liquor is as follows:
The Medium Proportion of B3, preparation seed liquor is as follows:
Peptone 350g, molasses 700g, potassium primary phosphate 45g, sodium hydrogen phosphate 42g, ferrous sulfate 5g, Vitamin C2 35g, the soya-bean milk 300kg that mass percent is 1:100, the corn steep liquor 50kg that mass percent is 1:100;
By cooling after above-mentioned medium sterilization, the shake-flask seed of making in the inoculum size that is 5% according to mass percent access step B2, fermentation is stirred in sealing oxygen supply, adopt hydrochloric acid or sodium hydroxide to adjust fermented liquid pH=7.5, after 24 hours, make seed liquor, leavening temperature is 29.5 DEG C-37.2 DEG C, and oxygen-supplying amount is 1:1, and mixing speed is 150 revs/min.
Industrial fermentation process step in described step B microbial inoculum preparation is as follows:
The Medium Proportion of B4, industrial fermentation is as follows:
Peptone 3.5g, molasses 7kg, potassium primary phosphate 910g, Sodium phosphate dibasic 840g, ferrous sulfate 35g, Vitamin C2 350g, the soya-bean milk 3000kg that mass percent is 1:100, the corn steep liquor 500kg that mass percent is 1:100;
By cooling after above-mentioned medium sterilization, the seed liquor of making in the inoculum size that is 10% according to mass percent access step B3, fermentation is stirred in sealing oxygen supply, adopt hydrochloric acid or sodium hydroxide to adjust fermented liquid pH=7.5, after 24 hours to bacterium number be the complete bacterium liquid that obtains of 10,000,000,000/ml fermentation, leavening temperature is 29.5 DEG C-37.2 DEG C, and oxygen-supplying amount is 1:1, and mixing speed is 150 revs/min.
In described step C, mixing dry bacterial powder is bacillus licheniformis agent according to ratio of quality and the number of copies: bacillus subtilis microbial agent: the ratio preparation of bacillus amyloliquefaciens microbial inoculum=2:1:1.
After dry in described step C, making mass percent moisture in biological organic fertilizer is 5%-8%.
Inorganic nitrogen in the bioprotein release and release control fertilizer that described step e is made: the ratio of quality and the number of copies of organonitrogen is 20:80, organonitrogen derives from biological organic fertilizer prepared by urea and step C, and inorganic nitrogen source is in monoammonium phosphate.
Embodiment 2
The difference of the present embodiment and embodiment 1 is:
In steps A, be rapeseed cake according to ratio of quality and the number of copies: the ratio of feather meal: bone meal: auxiliary material=35:35:22:22, by granulation shape fertilizer after above-mentioned raw materials mixture, pulverizing, auxiliary material is selected a kind of or its mixture in cottonseed cake or stalk;
The culture temperature of step B3, B4 is 29.5 DEG C.
C, mycoderm dressing
The microbial inoculum mixture of making in step B is made to mixing dry bacterial powder, be mixing dry bacterial powder according to ratio of quality and the number of copies: the ratio of carboxymethyl cellulose: water=5:0.05:3 is made liniment, it is liniment according to ratio of quality and the number of copies: the ratio of particulate state fertilizer=8.05:1000, liniment is coated on to the particulate state fertilizer surface of preparing in steps A, after being dried, makes biological organic fertilizer under 50 DEG C of conditions;
D, nitrification inhibitor dressing
It is nitrification inhibitor in ratio of quality and the number of copies: the ratio of No. 68 machinery oil=1:3, nitrification inhibitor is poured in No. 68 machinery oil, be warming up to 72.5 DEG C, being stirred to the chloro-6-trimethylpyridine of 2-dissolves, be under 72.5 DEG C of conditions in temperature, be nitrification inhibitor machine oil solution according to ratio of quality and the number of copies: the ratio of urea=1:1000, the nitrification inhibitor after dissolving is sprayed on to urea surface, nitrification inhibitor is selected N-serve;
The preparation of E, bioprotein release and release control fertilizer
It is nitrogen element according to biological bacteria 0.2 hundred million/g and ratio of quality and the number of copies: the ratio of phosphoric: potassium element: organic matter=28:4:8:16, granular organic fertilizer mixture prepared by biological organic fertilizer prepared by step C, nitrification inhibitor coated urea prepared by step D and monoammonium phosphate, Repone K or potassium sulfate and steps A is even, makes bioprotein release and release control fertilizer.
Inorganic nitrogen in the bioprotein release and release control fertilizer that step e is made: the ratio of quality and the number of copies of organonitrogen is 40:60, organonitrogen derives from biological organic fertilizer prepared by urea and step C, and inorganic nitrogen source is in monoammonium phosphate.
All the other contents are with embodiment 1.
Embodiment 3
The difference of the present embodiment and embodiment 1 is:
In steps A, be rapeseed cake according to ratio of quality and the number of copies: the ratio of feather meal: bone meal: auxiliary material=30:30:20:20, by granulation shape fertilizer after above-mentioned raw materials mixture, pulverizing, auxiliary material is selected a kind of or its mixture in cottonseed cake or stalk;
The culture temperature of step B3, B4 is 35 DEG C.
C, mycoderm dressing
The microbial inoculum mixture of making in step B is made to mixing dry bacterial powder, be mixing dry bacterial powder according to ratio of quality and the number of copies: the ratio of carboxymethyl cellulose: water=3:0.04:2 is made liniment, it is liniment according to ratio of quality and the number of copies: the ratio of particulate state fertilizer=6:745, liniment is coated on to the particulate state fertilizer surface of preparing in steps A, after being dried, makes biological organic fertilizer under 45 DEG C of conditions;
D, nitrification inhibitor dressing
It is nitrification inhibitor in ratio of quality and the number of copies: the ratio of No. 68 machinery oil=1:2.8, nitrification inhibitor is poured in No. 68 machinery oil, be warming up to 70 DEG C, being stirred to the chloro-6-trimethylpyridine of 2-dissolves, be under 70 DEG C of conditions in temperature, be the ratio of nitrification inhibitor oil solution: urea=0.98:980 according to ratio of quality and the number of copies, the nitrification inhibitor after dissolving is sprayed on to urea surface, nitrification inhibitor is selected N-serve;
The preparation of E, bioprotein release and release control fertilizer
It is nitrogen element according to biological bacteria 0.2 hundred million/g and ratio of quality and the number of copies: the ratio of phosphoric: potassium element: organic matter=20:20:5:18, biological organic fertilizer prepared by step C, nitrification inhibitor coated urea prepared by step D and, monoammonium phosphate, Repone K or potassium sulfate mixture even, make bioprotein release and release control fertilizer.
Inorganic nitrogen in the bioprotein release and release control fertilizer that step e is made: the ratio of quality and the number of copies of organonitrogen is 30:70, organonitrogen derives from biological organic fertilizer prepared by urea and step C, and inorganic nitrogen source is in monoammonium phosphate.
All the other contents are with embodiment 1.
Embodiment 4
The difference of the present embodiment and embodiment 1 is:
In steps A, be rapeseed cake according to ratio of quality and the number of copies: the ratio of feather meal: bone meal: auxiliary material=32:32:21:21, by granulation shape fertilizer after above-mentioned raw materials mixture, pulverizing, auxiliary material is selected a kind of or its mixture in cottonseed cake or stalk;
The culture temperature of step B3, B4 is 35 DEG C.
C, mycoderm dressing
The microbial inoculum mixture of making in step B is made to mixing dry bacterial powder, be mixing dry bacterial powder according to ratio of quality and the number of copies: the ratio of carboxymethyl cellulose: water=4:0.04:2.5 is made liniment, it is liniment according to ratio of quality and the number of copies: the ratio of particulate state fertilizer=6.8:845, liniment is coated on to the particulate state fertilizer surface of preparing in steps A, after being dried, makes biological organic fertilizer under 40 DEG C of conditions;
D, nitrification inhibitor dressing
It is nitrification inhibitor in ratio of quality and the number of copies: the ratio of No. 68 machinery oil=1:3, nitrification inhibitor is poured in No. 68 machinery oil, be warming up to 72 DEG C, being stirred to the chloro-6-trimethylpyridine of 2-dissolves, be under 72 DEG C of conditions in temperature, be the ratio of nitrification inhibitor oil solution: urea=1:1000 according to ratio of quality and the number of copies, the nitrification inhibitor after dissolving is sprayed on to urea surface, nitrification inhibitor is selected N-serve;
The preparation of E, bioprotein release and release control fertilizer
It is nitrogen element according to biological bacteria 0.2 hundred million/g and ratio of quality and the number of copies: the ratio of phosphoric: potassium element: organic matter=16:16:16:16, biological organic fertilizer prepared by step C, nitrification inhibitor coated urea prepared by step D and, monoammonium phosphate, Repone K or potassium sulfate mixture even, make bioprotein release and release control fertilizer.
Inorganic nitrogen in the bioprotein release and release control fertilizer that step e is made: the ratio of quality and the number of copies of organonitrogen is 25:75, organonitrogen derives from biological organic fertilizer prepared by urea and step C, and inorganic nitrogen source is in monoammonium phosphate.
All the other contents are with embodiment 1.
Embodiment 5
The difference of the present embodiment and embodiment 1 is:
In steps A, be rapeseed cake according to ratio of quality and the number of copies: the ratio of feather meal: bone meal: auxiliary material=28:28:18:18, by granulation shape fertilizer after above-mentioned raw materials mixture, pulverizing, auxiliary material is selected a kind of or its mixture in cottonseed cake or stalk;
The culture temperature of step B3, B4 is 32 DEG C.
C, mycoderm dressing
The microbial inoculum mixture of making in step B is made to mixing dry bacterial powder, be mixing dry bacterial powder according to ratio of quality and the number of copies: the ratio of carboxymethyl cellulose: water=4.5:0.05:2 is made liniment, it is liniment according to ratio of quality and the number of copies: the ratio of particulate state fertilizer=7.5:932, liniment is coated on to the particulate state fertilizer surface of preparing in steps A, after being dried, makes biological organic fertilizer under 50 DEG C of conditions;
D, nitrification inhibitor dressing
It is nitrification inhibitor in ratio of quality and the number of copies: the ratio of No. 68 machinery oil=0.9:2.8, nitrification inhibitor is poured in No. 68 machinery oil, be warming up to 71 DEG C, being stirred to the chloro-6-trimethylpyridine of 2-dissolves, be under 71 DEG C of conditions in temperature, be the ratio of nitrification inhibitor: urea=0.93:930 according to ratio of quality and the number of copies, the nitrification inhibitor after dissolving is sprayed on to urea surface, nitrification inhibitor is selected N-serve;
The preparation of E, bioprotein release and release control fertilizer
It is nitrogen element according to biological bacteria 0.2 hundred million/g and ratio of quality and the number of copies: the ratio of phosphoric: potassium element: organic matter=15:10:17:15, biological organic fertilizer prepared by step C, nitrification inhibitor coated urea prepared by step D and, monoammonium phosphate, Repone K or potassium sulfate mixture even, make bioprotein release and release control fertilizer.
Inorganic nitrogen in the bioprotein release and release control fertilizer that step e is made: the ratio of quality and the number of copies of organonitrogen is 35:65, organonitrogen derives from biological organic fertilizer prepared by urea and step C, and inorganic nitrogen source is in monoammonium phosphate.
All the other contents are with embodiment 1.
Claims (5)
1. the preparation method of multifunctional bio albumen release and release control fertilizer, is characterized in that comprising following processing step:
A, organic fertilizer granulation
Be rapeseed cake according to ratio of quality and the number of copies: the ratio of feather meal: bone meal: auxiliary material=25-35:25-35:18-22:18-22, by granulation shape fertilizer after above-mentioned raw materials mixture, pulverizing, auxiliary material is selected a kind of or its mixture in cottonseed cake or stalk;
B, microbial inoculum preparation
Accessing separately fermention medium by bacterial classification rejuvenation respectively, after spreading cultivation ferments, to after the bacterium liquid expansion drying after fermentation, make microbial inoculum, described bacterial classification is selected Bacillus licheniformis CCTCCNO:M2013021, subtilis CCTCCNO:M2013019 and bacillus amyloliquefaciens CCTCCNO:M2013020;
C, mycoderm dressing
The microbial inoculum mixture of making in step B is made to mixing dry bacterial powder, be mixing dry bacterial powder according to ratio of quality and the number of copies: the ratio of carboxymethyl cellulose: water=2.25-5:0.03-0.05:1.5-3 is made liniment, it is liniment according to ratio of quality and the number of copies: the ratio of particulate state fertilizer=3.78-8.05:470-1000, liniment is coated on to the particulate state fertilizer surface of preparing in steps A, after being dried, makes biological organic fertilizer under 40 DEG C of-50 DEG C of conditions;
D, nitrification inhibitor dressing
It is nitrification inhibitor in ratio of quality and the number of copies: the ratio of No. 68 machinery oil=0.8-1:2.6-3, nitrification inhibitor is poured in No. 68 machinery oil, be warming up to 68 DEG C-72.5 DEG C, being stirred to nitrification inhibitor dissolves, be under 68 DEG C of-72.5 DEG C of conditions in temperature, be nitrification inhibitor machine oil solution according to ratio of quality and the number of copies: the ratio of urea=0.98-1:980-1000, the nitrification inhibitor after dissolving is sprayed on to urea surface, nitrification inhibitor is selected N-serve;
The preparation of E, bioprotein release and release control fertilizer
It is nitrogen element according to biological bacteria 0.2 hundred million/g and ratio of quality and the number of copies: the ratio of phosphoric: potassium element: organic matter=13-28:4-20:5-17:15-20, granular organic fertilizer mixture prepared by biological organic fertilizer prepared by step C, nitrification inhibitor coated urea, monoammonium phosphate, Repone K or potassium sulfate prepared by step D and steps A is even, makes bioprotein release and release control fertilizer.
2. the preparation method of multifunctional bio albumen release and release control fertilizer according to claim 1, is characterized in that described step B microbial inoculum preparation comprises that the rejuvenation of bacterial classification spreads cultivation and makes preparation, the industrial fermentation of shake-flask seed, seed liquor.
3. the preparation method of multifunctional bio albumen release and release control fertilizer according to claim 1, is characterized in that in described step C, mixing dry bacterial powder is bacillus licheniformis agent according to ratio of quality and the number of copies: bacillus subtilis microbial agent: the ratio preparation of bacillus amyloliquefaciens microbial inoculum=2:1:1.
4. the preparation method of multifunctional bio albumen release and release control fertilizer according to claim 1, after being dried in the step C described in it is characterized in that, making mass percent moisture in biological organic fertilizer is 5%-8%.
5. the preparation method of multifunctional bio albumen release and release control fertilizer according to claim 1, inorganic nitrogen in the bioprotein release and release control fertilizer that step e described in it is characterized in that is made: the ratio of quality and the number of copies of organonitrogen is 20-40:60-80, organonitrogen derives from fertilizer and biological organic fertilizer prepared by urea and steps A, C, and inorganic nitrogen source is in monoammonium phosphate.
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| CN103553770B (en) * | 2013-10-21 | 2016-02-03 | 张喜梅 | A kind of ecological fertilizer and production method thereof containing organic microbial |
| CN104058818B (en) * | 2014-04-25 | 2015-12-02 | 浙江省农业科学院 | A kind of leaf vegetables Special slow release fertilizer |
| CN105272778A (en) * | 2015-11-11 | 2016-01-27 | 辽宁嘉丰科技有限公司 | Stably-blended fertilizer containing organic nutrients and manufacturing and using method of stably-blended fertilizer |
| CN106007955A (en) * | 2016-05-20 | 2016-10-12 | 深圳市芭田生态工程股份有限公司 | Hardening-preventing microorganism coated fertilizer |
| CN106106090A (en) * | 2016-06-28 | 2016-11-16 | 温振海 | A kind of Citrullus vulgaris culture matrix increasing the supply of root system oxygen |
| CN106866283A (en) * | 2017-04-06 | 2017-06-20 | 广州盈圃农业科技有限公司 | With greening beta pruning mucous membrane fertilizer is produced with ammonium metaphosphate industrial wastewater |
| CN108329124A (en) * | 2018-04-11 | 2018-07-27 | 河南沃特威生物科技有限公司 | A kind of biological organic fertilizer and preparation method thereof improving chemical fertilizer utilization ratio |
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| CN1480433A (en) * | 2002-09-03 | 2004-03-10 | 吴永常 | Fertilizer assorted with high grade organic matter |
| CN1480434A (en) * | 2002-09-03 | 2004-03-10 | 吴永常 | Organised fertilizer |
| CN1715399A (en) * | 2005-02-01 | 2006-01-04 | 湛江海洋大学 | Process for preparing lichem bacillus strain for producing composite amino acid and culture amino acid liquid fertilizer |
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| CN1480433A (en) * | 2002-09-03 | 2004-03-10 | 吴永常 | Fertilizer assorted with high grade organic matter |
| CN1480434A (en) * | 2002-09-03 | 2004-03-10 | 吴永常 | Organised fertilizer |
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