CN103068995B - 直接克隆 - Google Patents

直接克隆 Download PDF

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Publication number
CN103068995B
CN103068995B CN201180038908.6A CN201180038908A CN103068995B CN 103068995 B CN103068995 B CN 103068995B CN 201180038908 A CN201180038908 A CN 201180038908A CN 103068995 B CN103068995 B CN 103068995B
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nucleic acid
linearized
linear
acid molecule
rece
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CN103068995A (zh
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Y.张
J.付
F·斯图尔特
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Gene Bridges GmbH
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Gene Bridges GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/16—Exonucleases active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters (3.16)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201180038908.6A 2010-06-10 2011-06-10 直接克隆 Active CN103068995B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810875866.5A CN109825486A (zh) 2010-06-10 2011-06-10 用于进行同源重组的方法、宿主细胞和试剂盒

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1009732.7A GB201009732D0 (en) 2010-06-10 2010-06-10 Direct cloning
GB1009732.7 2010-06-10
PCT/IB2011/052549 WO2011154927A2 (en) 2010-06-10 2011-06-10 Direct cloning

Related Child Applications (1)

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CN201810875866.5A Division CN109825486A (zh) 2010-06-10 2011-06-10 用于进行同源重组的方法、宿主细胞和试剂盒

Publications (2)

Publication Number Publication Date
CN103068995A CN103068995A (zh) 2013-04-24
CN103068995B true CN103068995B (zh) 2020-10-16

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CN201180038908.6A Active CN103068995B (zh) 2010-06-10 2011-06-10 直接克隆
CN201810875866.5A Pending CN109825486A (zh) 2010-06-10 2011-06-10 用于进行同源重组的方法、宿主细胞和试剂盒

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CN201810875866.5A Pending CN109825486A (zh) 2010-06-10 2011-06-10 用于进行同源重组的方法、宿主细胞和试剂盒

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US (2) US10443051B2 (enExample)
EP (2) EP3138921A1 (enExample)
JP (2) JP5934196B2 (enExample)
CN (2) CN103068995B (enExample)
CA (1) CA2802167C (enExample)
GB (1) GB201009732D0 (enExample)
SG (1) SG186219A1 (enExample)
WO (1) WO2011154927A2 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201009732D0 (en) 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
EP2612918A1 (en) * 2012-01-06 2013-07-10 BASF Plant Science Company GmbH In planta recombination
CN102558309B (zh) * 2012-02-10 2014-09-17 浙江大学 一对转录激活子样效应因子核酸酶及其编码基因与应用
CN102627691B (zh) * 2012-04-05 2014-09-17 浙江大学 一对转录激活子样效应因子核酸酶及其编码基因与应用
JP6275120B2 (ja) * 2012-04-25 2018-02-07 リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． 大きい標的化ベクターによるヌクレアーゼ媒介標的化
CN102702335B (zh) * 2012-05-23 2014-07-09 上海斯丹赛生物技术有限公司 重组转录激活子样效应因子、转录激活子样效应因子核酸酶及其编码基因及应用
US20150267210A1 (en) 2012-10-16 2015-09-24 Genebridges Gmbh Electrocompetent cells and preparation thereof
EP3456831B1 (en) 2013-04-16 2021-07-14 Regeneron Pharmaceuticals, Inc. Targeted modification of rat genome
MX388127B (es) 2013-12-11 2025-03-19 Regeneron Pharma Metodos y composiciones para la modificacion dirigida de un genoma.
AU2014368982B2 (en) * 2013-12-19 2021-03-25 Amyris, Inc. Methods for genomic integration
BR112016028564A2 (pt) 2014-06-06 2018-01-30 Regeneron Pharma método para modificar um locus-alvo em uma célula.
MX384887B (es) 2014-06-23 2025-03-14 Regeneron Pharma Ensamblaje de adn mediado por nucleasa.
SG10201911411YA (en) 2014-06-26 2020-02-27 Regeneron Pharma Methods and compositions for targeted genetic modifications and methods of use
CA3176380A1 (en) 2014-11-21 2016-05-26 Regeneron Pharmaceuticals, Inc. Methods and compositions for targeted genetic modification using paired guide rnas
CN105713885B (zh) * 2014-12-04 2019-06-11 清华大学 特异识别并修复β地中海贫血症beta-globin基因的嵌合核酸酶
BR112017013104A2 (pt) * 2014-12-19 2018-05-15 Regeneron Pharma métodos para modificar um locus genômico alvo em uma célula, para intensificar a recombinação homóloga em um locus genômico alvo em uma célula e para produzir uma geração f0 de um animal não humano.
CN105734061B (zh) * 2016-03-25 2019-07-23 中国农业科学院生物技术研究所 从毕赤酵母中分离的鼠李糖诱导型启动子及其应用
CN109715813A (zh) * 2016-04-06 2019-05-03 基因桥有限公司 腺病毒载体
CN106995813B (zh) * 2017-03-23 2020-06-16 山东大学 基因组大片段直接克隆和dna多分子组装新技术
CN109385417A (zh) * 2017-08-03 2019-02-26 华东理工大学 体内dna无缝组装方法
MX2021012158A (es) * 2019-04-05 2022-01-06 Danisco Us Inc Métodos para la integración de una secuencia de adn donante en el genoma de bacillus usando constructos de adn recombinante lineal y composiciones de los mismos.
CN110184287B (zh) * 2019-05-24 2024-01-30 华南农业大学 一种制备重组病毒的方法及其应用
CN114630910A (zh) 2019-06-25 2022-06-14 伊纳瑞农业技术有限公司 改善的同源依赖修复基因组编辑
CN115717152A (zh) * 2022-11-29 2023-02-28 江南大学 一种控制枯草芽孢杆菌基因组同源重组概率的方法

Citations (2)

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CN1331748A (zh) * 1998-10-30 2002-01-16 默多克研究所 重组方法及其所用试剂
CN1373803A (zh) * 1999-07-09 2002-10-09 欧洲分子生物学实验室 应用同源重组定向克隆和亚克隆的方法和组合物

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US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5776744A (en) * 1995-06-07 1998-07-07 Yale University Methods and compositions for effecting homologous recombination
ATE449844T1 (de) 1997-12-05 2009-12-15 Europ Lab Molekularbiolog Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems
US6569681B1 (en) * 2000-03-14 2003-05-27 Transkaryotic Therapies, Inc. Methods of improving homologous recombination
EP1399546A2 (en) 2001-02-09 2004-03-24 Gene Bridges GmbH Recombination method
WO2003010322A1 (en) * 2001-07-20 2003-02-06 Youming Zhang Improved rect or recet cloning and subcloning method
EP1590468A2 (en) * 2003-01-28 2005-11-02 Cellectis Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof
WO2007013979A2 (en) 2005-07-20 2007-02-01 Invitrogen Corporation Methods for increasing efficiency of homologous recombination
KR100681815B1 (ko) * 2005-12-02 2007-02-12 한국생명공학연구원 리컴비네이즈 매개 선택표지 유전자 발현유도에 의한 목표유전자 간편 클로닝 및 목표 단백질 신속 발현 방법.
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CN1373803A (zh) * 1999-07-09 2002-10-09 欧洲分子生物学实验室 应用同源重组定向克隆和亚克隆的方法和组合物

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Publication number Publication date
US20130210681A1 (en) 2013-08-15
JP2013530689A (ja) 2013-08-01
WO2011154927A9 (en) 2013-01-24
US12195732B2 (en) 2025-01-14
CN103068995A (zh) 2013-04-24
WO2011154927A3 (en) 2012-02-16
SG186219A1 (en) 2013-01-30
JP5934196B2 (ja) 2016-06-15
CN109825486A (zh) 2019-05-31
JP2016135158A (ja) 2016-07-28
CA2802167C (en) 2023-03-07
US20200283759A1 (en) 2020-09-10
GB201009732D0 (en) 2010-07-21
WO2011154927A2 (en) 2011-12-15
EP3138921A1 (en) 2017-03-08
EP2580338B1 (en) 2016-10-05
CA2802167A1 (en) 2011-12-15
US10443051B2 (en) 2019-10-15
EP2580338A2 (en) 2013-04-17

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