CN103068854A - 具有因子vii活性的融合蛋白质 - Google Patents

具有因子vii活性的融合蛋白质 Download PDF

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Publication number
CN103068854A
CN103068854A CN2011800277076A CN201180027707A CN103068854A CN 103068854 A CN103068854 A CN 103068854A CN 2011800277076 A CN2011800277076 A CN 2011800277076A CN 201180027707 A CN201180027707 A CN 201180027707A CN 103068854 A CN103068854 A CN 103068854A
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CN
China
Prior art keywords
fvii
fused protein
seq
fusion protein
joint
Prior art date
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Pending
Application number
CN2011800277076A
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English (en)
Chinese (zh)
Inventor
宋寅瑛
金薰泽
李峰镛
朴万勋
李镐顺
林允挺
李智慧
孙瑞延
金旼善
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tiumbio Co ltd
Original Assignee
SK Chemicals Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SK Chemicals Co Ltd filed Critical SK Chemicals Co Ltd
Priority to CN201710403215.1A priority Critical patent/CN107188970A/zh
Publication of CN103068854A publication Critical patent/CN103068854A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/49—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/79—Transferrins, e.g. lactoferrins, ovotransferrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/31—Fusion polypeptide fusions, other than Fc, for prolonged plasma life, e.g. albumin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN2011800277076A 2010-06-04 2011-06-07 具有因子vii活性的融合蛋白质 Pending CN103068854A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710403215.1A CN107188970A (zh) 2010-06-04 2011-06-07 具有因子vii活性的融合蛋白质

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR10-2010-0052719 2010-06-04
KR20100052719 2010-06-04
PCT/KR2011/004131 WO2011152694A2 (en) 2010-06-04 2011-06-07 Fusion protein having factor vii activity

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201710403215.1A Division CN107188970A (zh) 2010-06-04 2011-06-07 具有因子vii活性的融合蛋白质

Publications (1)

Publication Number Publication Date
CN103068854A true CN103068854A (zh) 2013-04-24

Family

ID=45067214

Family Applications (2)

Application Number Title Priority Date Filing Date
CN2011800277076A Pending CN103068854A (zh) 2010-06-04 2011-06-07 具有因子vii活性的融合蛋白质
CN201710403215.1A Pending CN107188970A (zh) 2010-06-04 2011-06-07 具有因子vii活性的融合蛋白质

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201710403215.1A Pending CN107188970A (zh) 2010-06-04 2011-06-07 具有因子vii活性的融合蛋白质

Country Status (13)

Country Link
US (2) US9644197B2 (https=)
EP (1) EP2576625B1 (https=)
JP (2) JP6143297B2 (https=)
KR (1) KR101331101B1 (https=)
CN (2) CN103068854A (https=)
AU (1) AU2011262494B2 (https=)
BR (1) BR112012030941A2 (https=)
CA (1) CA2801223C (https=)
ES (1) ES2676874T3 (https=)
MX (1) MX348567B (https=)
RU (1) RU2585231C2 (https=)
WO (1) WO2011152694A2 (https=)
ZA (1) ZA201300066B (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2585231C2 (ru) 2010-06-04 2016-05-27 Ск Кемикалз Ко., Лтд. Слитый белок, обладающий активностью фактора vii
ES2630031T3 (es) * 2010-09-28 2017-08-17 Aegerion Pharmaceuticals, Inc. Un polipéptido de leptina pinnípedo-humano quimérico con solubilidad aumentada
US20140056870A1 (en) * 2012-08-27 2014-02-27 Allergan, Inc. Fusion proteins
KR20140106448A (ko) 2013-02-25 2014-09-03 에스케이케미칼주식회사 인자 vii을 포함하는 융합 단백질의 분리 및 정제 방법
KR102058864B1 (ko) * 2013-12-16 2019-12-24 주식회사 티움바이오 인자 vii의 융합 단백질을 포함하는 안정성이 개선된 약학적 조성물
EP3351263A1 (en) 2017-01-20 2018-07-25 Universitätsklinikum Hamburg-Eppendorf Pharmaceutical preparation for treating or preventing tissue adhesion
EP3668972B8 (en) 2017-08-18 2024-11-13 Neutrolis, Inc. Engineered dnase enzymes and use in therapy
US10988746B2 (en) 2018-10-08 2021-04-27 Neutrolis, Inc. Manufacturing and engineering of DNASE enzymes for therapy
AU2019358915B2 (en) 2018-10-08 2025-09-18 Neutrolis, Inc. Engineering of DNASE enzymes for manufacturing and therapy
CN110218259B (zh) * 2019-06-24 2022-09-16 王跃驹 植物生产胰高血糖素样肽-1短肽与转铁蛋白的融合蛋白制造口服降糖胶囊的应用
CN110229237A (zh) * 2019-06-24 2019-09-13 王跃驹 植物生产口服epo与转铁蛋白融合蛋白胶囊的应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1400910A (zh) * 2000-02-11 2003-03-05 马克西根公司 凝血因子VⅡ或VⅡa样分子
WO2003020746A1 (en) * 2001-08-30 2003-03-13 Biorexis Pharmaceutical Corporation Modified transferrin fusion proteins
CN1665526A (zh) * 2002-05-01 2005-09-07 舍林股份公司 作为抗凝剂的新的靶向组织因子的血栓调节蛋白融合蛋白
US20070072271A1 (en) * 2004-01-07 2007-03-29 Novo Nordisk Healthcare A/G Method for the production of recombinant proteins
CN101177456A (zh) * 2001-08-30 2008-05-14 比奥雷克西斯药物公司 经修饰的运铁蛋白融合蛋白

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US8129504B2 (en) 2001-08-30 2012-03-06 Biorexis Technology, Inc. Oral delivery of modified transferrin fusion proteins
US7176278B2 (en) * 2001-08-30 2007-02-13 Biorexis Technology, Inc. Modified transferrin fusion proteins
US7125843B2 (en) * 2001-10-19 2006-10-24 Neose Technologies, Inc. Glycoconjugates including more than one peptide
EP1545595B1 (en) * 2002-08-30 2010-07-07 Biorexis Pharmaceutical Corporation Modified transferrin fusion proteins comprising duplicate transferrin amino or carboxy terminal domains
EP1816201A1 (en) 2006-02-06 2007-08-08 CSL Behring GmbH Modified coagulation factor VIIa with extended half-life
EP2004683B1 (en) * 2006-03-24 2016-05-11 Biogen Hemophilia Inc. Pc5 as a factor ix propeptide processing enzyme
GB0614780D0 (en) * 2006-07-25 2006-09-06 Ucb Sa Biological products
WO2009086132A2 (en) 2007-12-20 2009-07-09 University Of Southern California Design of spacers to increase the expression of recombinant fusion proteins
EP3412300A1 (en) 2008-06-27 2018-12-12 Duke University Therapeutic agents comprising elastin-like peptides
EP2509502A2 (en) * 2009-12-08 2012-10-17 Koninklijke Philips Electronics N.V. A method and a correction system for correcting tracer-uptake measurements
RU2585231C2 (ru) 2010-06-04 2016-05-27 Ск Кемикалз Ко., Лтд. Слитый белок, обладающий активностью фактора vii

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1400910A (zh) * 2000-02-11 2003-03-05 马克西根公司 凝血因子VⅡ或VⅡa样分子
WO2003020746A1 (en) * 2001-08-30 2003-03-13 Biorexis Pharmaceutical Corporation Modified transferrin fusion proteins
CN101177456A (zh) * 2001-08-30 2008-05-14 比奥雷克西斯药物公司 经修饰的运铁蛋白融合蛋白
CN1665526A (zh) * 2002-05-01 2005-09-07 舍林股份公司 作为抗凝剂的新的靶向组织因子的血栓调节蛋白融合蛋白
US20070072271A1 (en) * 2004-01-07 2007-03-29 Novo Nordisk Healthcare A/G Method for the production of recombinant proteins

Also Published As

Publication number Publication date
CA2801223C (en) 2019-12-03
EP2576625A4 (en) 2013-11-06
JP2017000144A (ja) 2017-01-05
EP2576625A2 (en) 2013-04-10
ES2676874T3 (es) 2018-07-25
MX348567B (es) 2017-06-20
KR20110133454A (ko) 2011-12-12
US20130236945A1 (en) 2013-09-12
US20160194622A1 (en) 2016-07-07
RU2585231C2 (ru) 2016-05-27
JP6143297B2 (ja) 2017-06-07
JP2013529914A (ja) 2013-07-25
CN107188970A (zh) 2017-09-22
AU2011262494A1 (en) 2013-01-24
CA2801223A1 (en) 2011-12-08
US10696960B2 (en) 2020-06-30
WO2011152694A8 (en) 2013-01-17
EP2576625B1 (en) 2018-04-25
ZA201300066B (en) 2014-03-26
MX2012013807A (es) 2012-12-17
BR112012030941A2 (pt) 2017-06-06
US9644197B2 (en) 2017-05-09
KR101331101B1 (ko) 2013-11-19
RU2012157301A (ru) 2014-07-20
WO2011152694A3 (en) 2012-04-19
AU2011262494A2 (en) 2013-08-01
JP6422918B2 (ja) 2018-11-14
AU2011262494B2 (en) 2016-01-21
WO2011152694A2 (en) 2011-12-08

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SE01 Entry into force of request for substantive examination
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TA01 Transfer of patent application right

Effective date of registration: 20170605

Address after: Gyeonggi Do, South Korea

Applicant after: TiumBio Co.,Ltd.

Address before: Gyeonggi Do, South Korea

Applicant before: SK CHEMICALS Co.,Ltd.

RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20130424