CN103044094A - Microorganism active phosphate fertilizer prepared from water hyacinth residue and composite microorganism strain - Google Patents
Microorganism active phosphate fertilizer prepared from water hyacinth residue and composite microorganism strain Download PDFInfo
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- CN103044094A CN103044094A CN2012105771069A CN201210577106A CN103044094A CN 103044094 A CN103044094 A CN 103044094A CN 2012105771069 A CN2012105771069 A CN 2012105771069A CN 201210577106 A CN201210577106 A CN 201210577106A CN 103044094 A CN103044094 A CN 103044094A
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Abstract
The invention relates to a microorganism active phosphate fertilizer prepared from water hyacinth residue and a composite microorganism strain and belongs to the technical field of microorganism organic phosphate fertilizer. The microorganism active phosphate fertilizer has a component weight percentage ratio of a water hyacinth residue organic phosphate fertilizer to a composite microorganism bacterium agent of 1:(0.1 to 0.2), wherein components of the water hyacinth residue organic phosphate fertilizer have a weight percentage ratio of the water hyacinth residue to ground phosphate rock with fineness of 300 to 350 mesh to agrocybe cylindracea mushroom residue to tobacco powder to Bacillus cereus KMSR88 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6596 of 6:1.5:1.5:0.8:0.01; and the composite microorganism bacterium agent is prepared from a Pseudomonas fluorescens KMXU22 strain with the preservation number of CGMCC No.6597 and a Bacillus amyloliquefaciens KMXU1 strain with the preservation number of CCMCC No.6598 by a conventional method. The microorganism active phosphate fertilizer has the advantages that 1, the microorganism active phosphate fertilize is low in cost, is convenient to use, has obvious effects, is nontoxic, is harmless and has no pollution to the environment; and 2, physical and chemical properties of soil with continuous cropping obstacles and soil microbial activity can be improved, stress resistance of plants is improved, and the microorganism organic phosphate fertilizer has effects of durable fertilizer efficiency and reduction of pest and disease damage.
Description
Technical field:
The present invention relates to a kind of microorganism active phosphate fertilizer with Herba Eichhorniae slag and the preparation of complex microorganism bacterial classification, microorganism belonging to genus organic phosphatic fertiliser technical field.
Background technology:
Herba Eichhorniae has another name called Herba Eichhorniae, and belong to the Pontederiaceae Herba Eichhorniae and belong to, be a kind of perennial floating plant that originates in South America.Herba Eichhorniae natural disposition happiness light is adapted to the torrid zone, subtropical climate (27-30 ℃ is optimum growth temp), fast growth (Sheng Jing etc., 2010).Herba Eichhorniae is abundant in china natural resources.Because the Herba Eichhorniae big area covers, easily cause the detrimentally affects such as Dissolved Oxygen in Water reduction, species diversity minimizing, waterborne traffic obstruction, a lot of areas, annual south all need actively to take measures it is salvaged prevents and kill off, often directly abandon after but Herba Eichhorniae is salvaged, cause serious secondary pollution and the wasting of resources (Liu Hongjiang etc. 2011).Studies have shown that Herba Eichhorniae has higher utility value.Gajalakshmi and Abbasi (2002) result of study shows, the green manure that utilizes the Herba Eichhorniae hybrid composting to produce produces obvious facilitation effect to the growth of plant, and is safe and feasible.Murugesan etc. show that take Herba Eichhorniae, straw as raw material culturing edible fungus, result the income that Herba Eichhorniae is processed is significantly higher than straw and processes.Trans-utilization by Herba Eichhorniae reduces Herba Eichhorniae quantity, and making it turns waste into wealth, and is the good approach of the water body harm of control Herba Eichhorniae and secondary pollution.Existing studies show that, Herba Eichhorniae are one of the highest waterplant of potassium content (Reddy et al., 1991; Zhou Wenbing etc., 2004).But the Herba Eichhorniae slag is the soil utilization after compost treatment, can take full advantage of its organic and nutritive elements such as nitrogen, phosphorus, is the disposal options that is fit to China's national situation.
The phosphate fertilizer of our general using is via the produced fertilizer of chemical process at present, and it is via the vitriol oil form that phosphorus ore resolves into the higher calcium superphosphate of solubility to be used as the phosphate fertilizer sale.Cause atmospheric pollution in the air because in making the phosphate fertilizer process, having the sulfurous gas sulfides to be emitted on, the sulfate radical that can bring because of phosphate fertilizer during use is soil acidification, and the phosphate fertilizer more than 80 percent is fixed by elements such as the aluminium in the soil, iron, calcium, magnesium and made the soil salinization.Phosphate fertilizer under general situation in soil actual by crop (plant) utilize less than 20%.The shortcoming of ploughing and planting and more worsen for solving above atmospheric pollution, soil acidification, salinization and utilization ratio problem on the low side.Therefore organic fertilizer is used, and to higher yield of crops, high-quality, strengthens agricultural aftereffect, improves farmland ecological environment, and it is out of proportion significant to alleviate chemical fertilizer.Along with the development of social progress and science and technology, people more and more pay attention to the Sustainable development of quality safety, environment protection and the agricultural of agricultural-food.
A kind of microorganism active phosphate fertilizer with Herba Eichhorniae slag and the preparation of complex microorganism bacterial classification of the present invention by literature search, has no the open report identical with the present invention.
Reference:
[1]?Reddy?K.R.,?Agami?M.,?D'Angelo?E.M.,?Tucker?J.C，Influence?of?K?supply?on?growth?and?nutrient?storage?of?water?hyacinth.?Bioresource?Technol.?,?1991,?37?(1)：79–84
[2] Liu Hongjiang keeps root, and Zhu Puping contains Jing, Zhang Yuefang, and Zheng Jianchu, farmland application of water cucurbit uses and the environmental organism journal 2011,17 (4): 521-526 to the impact of rice dry matter production and distribution
[3] contain Jing, keep root, Zhu Puping, Zhou Wei, Xue Xinhong, the farmland utilization research of high eutrophication vegetation water cucurbit, Chinese Ecological Agriculture journal, 2010,18 (1): 46-49
[4] thank to the Jianchang, Zhou Jianmin, Harder R. potassium and Chinese agriculture. Nanjing: press of Hohai University, 2000:105-106
[5] Zhou Wenbing, Liu Dahui, Zhu Duanwei, different amendments be on the impact of pig manure process and nutrient situation thereof, Hua Zhong Agriculture University's journal, 2004,23 (4): 421 – 425
Summary of the invention:
The object of the present invention is to provide a kind of microorganism active phosphate fertilizer with Herba Eichhorniae slag and the preparation of complex microorganism bacterial classification.
The object of the present invention is achieved like this:
A kind of microorganism active phosphate fertilizer with Herba Eichhorniae slag and the preparation of complex microorganism bacterial classification, the weight percentages of components of this microorganism organic phosphatic fertiliser is: Herba Eichhorniae slag organic phosphatic fertiliser: complex micro organism fungicide=1:0.1-0.2.
The weight percentages of components of described Herba Eichhorniae slag organic phosphatic fertiliser is: the Herba Eichhorniae slag: fineness 300-350 purpose ground phosphate rock: tea tree mushroom bacterium slag: offal: preserving number is bacillus cereus (Bacillus cereus) KMSR88:=6:1.5:1.5:0.8:0.01 of CGMCC No.6596; Wherein:
Described Herba Eichhorniae slag is the reed slag that the fresh water cucurbit is crushed to 3-5cm;
Described ground phosphate rock is that the mid low grade phosphate rock of phosphorus content 20-25% is broken into fineness 300-350 purpose breeze;
Described offal is: the offal that obtains after the offal of tobacco leaf is pulverized in cigarette factory, the Redrying Factory.
Described complex micro organism fungicide obtains through following steps:
With preserving number be CGMCC NO. 6597 fluorescent pseudomonas (Pseudomonas fluorescens) KMXU22 bacterial strain, and preserving number be bacillus amyloliquefaciens (Bacillus amyloliquefaciens) the KMXU1 bacterial strain of CGMCC NO. 6598, seed culture and fermentation culture through routine are made bacteria suspension, then be sprayed on the Semen Maydis powder carrier, microbe population reaches 1 * 10
11Individual/more than the g, oven dry below 65 ℃ obtains the solid composite microbe microbial inoculum.
Preserving number is bacillus cereus (Bacillus cereus) KMSR88 of CGMCC No.6596, preserving number is fluorescent pseudomonas (Pseudomonas fluorescens) the KMXU22 bacterial strain of CGMCC NO. 6597, and preserving number is bacillus amyloliquefaciens (Bacillus amyloliquefaciens) the KMXU1 bacterial strain of CGMCC NO. 6598, all be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on September 21st, 2012 and (be called for short CGMCC, address: No. 3, No. 1 institute in BeiChen West Road, Chaoyang District, BeiJing City, Institute of Microorganism, Academia Sinica, postcode 100101).
The preparation method of a kind of microorganism active phosphate fertilizer with the preparation of Herba Eichhorniae slag and complex microorganism bacterial classification of the present invention is ordinary method, and equipment used is the general-purpose equipment of market sale.
The invention has the advantages that:
1, cost is low, and is easy to use, and successful is nontoxic, harmless, pollute without environment.
2, this fertilizer can not only replenish the required nutritive element of plant growth, can also improve physicochemical character and the soil microbial activities of continuous cropping obstacle soil, improves the resistance of plant, and has fertilizer efficiency to reach lastingly the effect that reduces disease and pest.
Embodiment:
The used preparation method of embodiment is ordinary method, and equipment used is the general-purpose equipment of market sale.
Embodiment 1:
1. the preparation of complex micro organism fungicide
Fluorescent pseudomonas (Pseudomonas fluorescens) and bacillus amyloliquefaciens (Bacillus amyloliquefaciens) are transplanted to respectively the test tube that contains the LB substratum carry out a ring activation, cultivated 48 hours for 29 ℃, the LB culture medium prescription is: Tryptones (Tryptone) 10g/L, yeast extract (Yeast extract) 5g/L, sodium-chlor (NaCl) 5g/L, pH7.0, agar 17g/L; Get lawn after the ring activation and move in the LB nutrient solution with the bottled 600ml of 1000ml taper, turn 110/min, 29 ℃ of lower cultivations 72 hours, obtain zymocyte liquid; The training zymocyte liquid is inoculated in fermentation culture in the LB nutrient solution in the fermentor tank in the ratio of inoculum size 1:20 training, and 29-31 ℃ of fermentation is after 72-96 hour, and bacterial content is 1 * 10 in the fermented liquid in the fermentor tank
11During the above bacterium liquid of individual/g, go out tank stores.Then fluorescent pseudomonas and bacillus amyloliquefaciens 1:1 evenly are mixed to get composite microbial bacteria liquid; Cultured composite microbial bacteria liquid is sprayed on the Semen Maydis powder carrier, and microbe population reaches 1 * 10
11Individual/when g was above, bake drying packaged spare below 65 ℃.
2. the preparation of Herba Eichhorniae slag organic phosphatic fertiliser
With the Herba Eichhorniae slag: fineness 300-350 purpose ground phosphate rock: tea tree mushroom bacterium slag: offal: preserving number is that the weight ratio of bacillus cereus (Bacillus cereus) KMSR88:=6:1.5:1.5:0.8:0.01 of CGMCC No.6596 mixes evenly that to regulate carbon-nitrogen ratio be 25, water ratio 56%, carry out compost, the compost cycle is 22 days, measure compost temperature every day, after rising to 60 ℃ and lasting 24 hours, compost temperature carries out turning, continue fermentation after the turning, until material is when being grey chocolate, the material fermenting-ripening.The material of fermenting-ripening, through the tunnel kiln drying, temperature is controlled at below 75 ℃, and water content is controlled in the 10-15% scope, crushes and screens, and fineness reaches the 40-50 order, and is for subsequent use.
4. Herba Eichhorniae slag microorganism organic phosphatic fertiliser preparation
The complex micro organism fungicide for preparing is joined in the Herba Eichhorniae slag organic phosphatic fertiliser by 0.1% or 0.2% part by weight, mix, obtain microorganism active phosphate fertilizer after the quantitative package, the total viable count in this microorganism active phosphate fertilizer is greater than 0.2 hundred million/gram.
Claims (3)
1. the microorganism active phosphate fertilizer with Herba Eichhorniae slag and the preparation of complex microorganism bacterial classification is characterized in that the weight percentages of components of this microorganism organic phosphatic fertiliser is, Herba Eichhorniae slag organic phosphatic fertiliser: complex micro organism fungicide=1:0.1-0.2.
2. the microorganism active phosphate fertilizer with the preparation of Herba Eichhorniae slag and complex microorganism bacterial classification claimed in claim 1, it is characterized in that the weight percentages of components of described Herba Eichhorniae slag organic phosphatic fertiliser is: Herba Eichhorniae slag: ground phosphate rock: tea tree mushroom bacterium slag: offal: preserving number is bacillus cereus (Bacillus cereus) KMSR88=6:1.5:1.5:0.8:0.01 of CGMCC No.6596; Wherein:
Described Herba Eichhorniae slag is the reed slag that the fresh water cucurbit is crushed to 3-5cm;
Described ground phosphate rock is that the mid low grade phosphate rock of phosphorus content 20-25% is broken into fineness 300-350 purpose breeze;
Described offal is: the offal that obtains after the offal of tobacco leaf is pulverized in cigarette factory, the Redrying Factory.
3. the microorganism active phosphate fertilizer with the preparation of Herba Eichhorniae slag and complex microorganism bacterial classification claimed in claim 1 is characterized in that described complex micro organism fungicide obtains through following steps:
With preserving number be CGMCC NO. 6597 fluorescent pseudomonas (Pseudomonas fluorescens) KMXU22 bacterial strain, and preserving number be bacillus amyloliquefaciens (Bacillus amyloliquefaciens) the KMXU1 bacterial strain of CGMCC NO. 6598, make bacteria suspension through seed culture and fermentation culture, then be sprayed on the Semen Maydis powder carrier, microbe population reaches 1 * 10
11Individual/when g was above, oven dry obtained the solid composite microbe microbial inoculum.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104355820A (en) * | 2014-10-31 | 2015-02-18 | 云南省烟草公司玉溪公司 | Water hyacinth containing adsorbent composition for microbial fertilizer |
| CN110885771A (en) * | 2019-11-22 | 2020-03-17 | 云南省烟草公司临沧市公司 | Biological agent for improving tobacco-planting soil micro-ecological environment level |
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| CN102268390A (en) * | 2011-07-07 | 2011-12-07 | 华中农业大学 | Bacillus amyloliquefaciens for generating fungi apoptosis lipopeptide, and preparation method and application thereof |
| CN102674934A (en) * | 2012-05-28 | 2012-09-19 | 昆明学院 | Water-retaining amino acid bio-organic fertilizer prepared prepare from water hyacinth |
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Patent Citations (5)
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| CN1923760A (en) * | 2006-09-14 | 2007-03-07 | 广东琪田农药化工有限公司 | Biological activity fertilizer produced from hyacinth fermentation slag and method thereof |
| CN101486613A (en) * | 2009-02-18 | 2009-07-22 | 沃邦（江苏）生态肥业有限公司 | Method for preparing organic fertilizer by mixed fermentation of water hyacinth and dewatered sludge |
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| CN102268390A (en) * | 2011-07-07 | 2011-12-07 | 华中农业大学 | Bacillus amyloliquefaciens for generating fungi apoptosis lipopeptide, and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104355820A (en) * | 2014-10-31 | 2015-02-18 | 云南省烟草公司玉溪公司 | Water hyacinth containing adsorbent composition for microbial fertilizer |
| CN104355820B (en) * | 2014-10-31 | 2018-04-27 | 云南省烟草公司玉溪市公司 | A kind of microbial manure adsorbent composition containing water hyacinth |
| CN110885771A (en) * | 2019-11-22 | 2020-03-17 | 云南省烟草公司临沧市公司 | Biological agent for improving tobacco-planting soil micro-ecological environment level |
| CN110885771B (en) * | 2019-11-22 | 2023-05-16 | 云南省烟草公司临沧市公司 | Biological microbial agent for improving micro-ecological environment level of tobacco planting soil |
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Application publication date: 20130417 |