CN103039157A - Rapid after-ripening and germination method of pulsatilla koreana seeds - Google Patents
Rapid after-ripening and germination method of pulsatilla koreana seeds Download PDFInfo
- Publication number
- CN103039157A CN103039157A CN2013100189678A CN201310018967A CN103039157A CN 103039157 A CN103039157 A CN 103039157A CN 2013100189678 A CN2013100189678 A CN 2013100189678A CN 201310018967 A CN201310018967 A CN 201310018967A CN 103039157 A CN103039157 A CN 103039157A
- Authority
- CN
- China
- Prior art keywords
- ripening
- seeds
- seed
- germination
- rapid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention relates to a plant propagation technology and provides a rapid after-ripening and germination method of pulsatilla koreana seeds. The rapid after-ripening and germination method comprises the following steps of: immersing the pulsatilla koreana seeds by utilizing a solution mixed by KT with the concentration of 2.10-2.30mg/L, GA3 with the concentration of 2.90-3.20mg/L and 2,4-D with the concentration of 0.30-0.60mg/L; uniformly mixing the seeds with river sand and putting the mixture into a wood groove; placing the wood groove into a room to carry out poikilothermic treatment for 24-28 days, wherein the poikilothermic treatment amplitude and time are as follows: the temperature at daytime is 25+/-2 DEG C and the treatment time is 14 hours; and the temperature at night is 10+/-2 DEG C and the treatment time is 10 hours; and treating for 24-28 days and directly sowing the seeds. The pulsatilla koreana seeds can germinate after being treated by the method and sowed for 10-14 days; and the germination rate is more than 99.9%. The rapid after-ripening and germination method of the pulsatilla koreana seeds has the characteristics of short seed treatment time, strong maneuverability, very high germination rate and the like. The rapid after-ripening and germination method belongs to a rapid after-ripening and efficient seedling raising technique and can be popularized and applied to industrial seedling raising and scaled cultivation of pulsatilla koreana.
Description
Technical field
The present invention relates to a kind of plant propagation technology, namely
OnePlant the quick after-ripening of pasque flower seed and germination method.
Background technology
In the prior art, pasque flower [
Pulsatilla cernua(Thunb.) Bercht. et Opiz.], Ranunculaceae Pulsatilla perennial root herbaceous plant, root contains root of Chinese clematis table disaccharides saponin, root of Chinese clematis disaccharides saponin, the important chemical compositions such as saponin II and saponin III, have the effects such as purging intense heat and detonicating, pain relieving, inducing diuresis to reduce edema, cure mainly the diseases such as amoebic dysentery, malaria, metal-inflicted wound, women's amenorrhoea, acute toothache, extremities joint pain, the favus of the scalp and edema, external application can be controlled the white disease such as bald of external hemorrhoid welling and pain, children's; Also can make desinsection and botanical pesticide.Pasque flower is often rare in Changbaishan area quantity, is the genuine medicinal plant of Changbai Mountain preciousness, is decided to be a provincial class and lays special stress on protecting plant in " Jilin Wild fauna and flora protection provisional administrative regulations " literary composition.
Pasque flower is distributed in northeast short high and cold area of frost-free season more, autumn, behind the seed collection, embryo in the seed is in the cotyledonary embryos mid-term stage, need to carry out after-ripening and process, otherwise sprouting is extremely low behind the planting seed, and the vegetative propagation rates such as root division are extremely low, moreover cause wild resource to be subject to extinctive threat, extensive artificial cultivation, development and utilization are extremely restricted.
Summary of the invention
The objective of the invention is to provide for above-mentioned deficiency a kind of seed afterripening time short, workable, germination rate is high
OnePlant the quick after-ripening of pasque flower seed and germination method.
Technical solution of the present invention is: the quick after-ripening of a kind of pasque flower seed and germination method is characterized in that step is as follows:
The pasque flower seed is with 2.10～2.30 mgL
-1Kinetin (KT), 2.90～3.20 mgL
-1Gibberellin (GA
3) and 0.30～0.60 mgL
-12, the 4-dichlorphenoxyacetic acid (2, after 4-D) mixed solution soaks, seed and river sand are mixed in the trogue of all packing into afterwards, place the indoor alternating temperature processing of carrying out 24～28 d; Alternating temperature is processed amplitude and time: day temperature is 25 ± 2 ℃, processes 14 h, and nocturnal temperature is 10 ± 2 ℃, processes 10 h; Processed 24～28 d(days) after can directly sow.
Seed soak time 18 h(hours).Seed: river sand=1:2.
Advantage of the present invention is: 1, the present invention takes the mode of an artificial after-ripening of step that seed is processed, and reaching the target of the quick after-ripening of seed and sprouting, solves the in the past artificial after-ripening complexity of taking steps, and speed waits problem slowly.2, be KT, GA through testing the factor of having determined to affect the quick after-ripening of seed
3, 2,4-D, alternating temperature process and the processing time, and by KT, GA
3, 2, the seed that 4-D and alternating temperature are processed no longer needs the freezing lamination can direct fast-germination.After this method processing and sowing 10～14 d, the pasque flower seed can germinate, and germination rate reaches more than 99.9%.This method not only solved planting seed sprout difficult, and extremely low difficult points of vegetative propagation rate such as root division, and overcome report in the past and study in complicated processing procedure.3, this method has and processes short, workable, the advantage such as germination rate is high of pasque flower seed time.Belong to quick after-ripening and high efficiency seedling cultivating technology, can be applied to factorial seedling growth and the large-scale planting of pasque flower.
Below in conjunction with embodiment embodiments of the present invention are described in further detail.
Embodiment
OnePlant the quick after-ripening of pasque flower seed and germination method:
1 materials and methods
1.1 experiment material and processing
Gather the pasque flower seed autumn (mid-October), and (concentration is controlled at 1.50～2.00 mgL to remove behind the white cilium that covers on the seed directly kinetin (KT) with different quality concentration
-1), gibberellin (GA
3) (concentration is controlled at 2.20～2.70 mgL
-1) and 2,4-dichlorphenoxyacetic acid (2,4-D) (concentration is controlled at 0.80～1.30 mgL
-1) soak 18 h in the mixed solution, again seed and river sand (1:2) to be mixed in the trogue of all packing into afterwards, covered with plastic film places the indoor alternating temperature that carries out to process, and maintenance relative moisture is controlled at about 65%～70%.
1.2 the after-ripening of pasque flower seed, sowing and management
The beginning alternating temperature is processed after through method in 1.1 seed being soaked vanning, and alternating temperature is processed and is divided into two stages, and alternating temperature amplitude and time: day temperature is 25 ± 2 ℃, processes 14 h, and nocturnal temperature is 10 ± 2 ℃, processes 10 h.Processing the time limit is 14～24 d.Percentage by per 100 seed germinations is investigation object, i.e. germination rate=seed germination number/100 * 100%.For improving pasque flower seed germination rate and sprouting speed, by the Uniform Design method experiment, select U
12(12
4) evenly show, each processes 100 seeds, repeats 3 times and averages, and determines in the pasque flower seed afterripening process KT, GA
3, 2, the best in quality concentration of 4-D solution and suitable treatment time.
Pasque flower seed by above processing can disseminate on ready-made soil bed (furrow) together with river sand Direct Uniform, and overlying soil thickness is the fine earth about 0.5 cm, waters permeablely, treats seed sprouting.
After seed sprouting is unearthed, note temperature, relative moisture, illumination, keep ventilation, suitably spray an amount of antibacterials preventing the generation of root rot, and notice that fertilising removes weeds.
2 results and analysis
2.1 KT, GA
3, 2,4-D concentration is intersected the impact on the pasque flower seed germination of proportioning and alternating temperature processing time
Table 1
Affect the U of pasque flower seed germination principal element
12(12
4) experimental scheme and result
Can get regression equation after data (table 1) are processed by analysis is
Y=-102+37.0
X 1+ 25.7
X 2-25.3
X 3+ 8.80
X 4-0.231
X 4 2, significance
α=0.05, multiple correlation coefficient
R=0.9968, residual standard deviation
S=0.8670, test value
F t =187.5 ﹥ critical values
F (0.05,5,6)=4.387, regression equation has significance.KT, GA
3, 2,4-D and alternating temperature processing time all have appreciable impact to the pasque flower Seed germination.By calculating K T, GA
3, 2,4-D and alternating temperature processing time to the contribution margin of pasque flower seed germination and contribution rate as can be known,
U 1=328,
U 1/
U=46.6%;
U 2=114,
U 2/
U=16.2%;
U 3=154,
U 3/
U=21.8%;
U 4=63.4,
U 4/
U=8.98%;
U 5=63.6,
U 5/
U=9.02%, illustrate that 4 factors to the contribution of pasque flower seed germination sequentially are: KT〉2,4-D〉GA
3The alternating temperature processing time, again because of KT, GA
3Be proportionate with alternating temperature processing time and cotyledonary embryos incidence, and 2,4-D and alternating temperature processing time square with germination rate be negative correlation.Though the alternating temperature processing time is little to the contribution of pasque flower seed germination rate, the alternating temperature processing time is played indispensable effect equally in the pasque flower during Seed Germination.Infer thus KT and GA
3Mass concentration is higher than respectively 2.00 mgL
-1With 2. 70 mgL
-1, 2,4-D mass concentration is lower than 0.80 mgL
-1, the alternating temperature processing time, the pasque flower seed germination rate may be higher when surpassing 24 d.For verifying this possibility, again take the KT mass concentration as 2.00,2.10,2.20,2.30,2.40 and 2.50 mgL
-1, GA
3Mass concentration is 2.70,2.80,2.90,3.00,3.10,3.20,3.30,3.40 and 3.50 mgL
-1, 2,4-D mass concentration is 0.80,0.70,0.60,0.50,0.40,0.30,0.20 and 0.10 mgL
-1, the alternating temperature processing time is the demonstration test that 24,26,28,30,32,34 and 36 d carry out 9 levels, repeats 3 times.Found that KT, GA
3Be respectively (2.10～2.30 mgL with 2,4-D mass concentration scope
-1), (2.90～3.20 mgL
-1) and (0.30～0.60 mgL
-1) and alternating temperature processing time when being (24～28 d), the pasque flower seed germination rate is the highest, and average germination rate reaches more than 99.9%.The germination rate that all is higher than listed 12 processing of table 1.
2.2 the sowing after the pasque flower seed treatment and management
After seed sprouting was unearthed, temperature remained on 18～20 ℃, and relative moisture is 70%, lighting delay number position 10 hd
-1Intensity of illumination is controlled at 1000～1200 lx, keep ventilation, the first frost of 1 800～1000 times of liquid of per 10 d spraying is disliked mould spirit to prevent the generation of root rot, till becoming redness to the stem below the cotyledon, per 15 d spray 3.0% potassium dihydrogen phosphate 1 time on blade, and note removing in early days weeds.
3 conclusions
The factor and the scope that affect the pasque flower seed germination are respectively KT (2.10～2.30 mgL
-1), GA
3Mass concentration is (2.90～3.20 mgL
-1), 2,4-D (0.30～0.60 mgL
-1) and the alternating temperature processing time be (24～28 d).
Therefore, can determine that pasque flower seed afterripening and the condition of sprouting are: seed is with 2.10～2.30 mgL
-1KT, 2.90～3.20 mgL
-1GA
3With 0.30～0.60 mgL
-12, after the 4-D mixed solution soaks 18 h, add river sand seed and river sand (1:2) mixed in the trogue of all packing into afterwards, covered with plastic film places the indoor alternating temperature processing of carrying out 24～28 d.Alternating temperature is processed amplitude and time: day temperature is 25 ± 2 ℃, processes 14 h, and nocturnal temperature is 10 ± 2 ℃, processes 10 h.Can directly sow after processing 24～28 d.
After processing and sow 10～14 d according to method in 1.1 and 1.2, the pasque flower seed can germinate, and germination rate reaches more than 99.9%.This method has not only solved planting seed and has sprouted difficulty, and the extremely low difficult points of vegetative propagation rate such as root division, and overcome report in the past and study in complicated processing procedure, this method has short, workable, the advantage such as germination rate is high of the pasque flower seed time of processing.Belong to quick after-ripening and high efficiency seedling cultivating technology, can be applied to factorial seedling growth and the large-scale planting of pasque flower.
Claims (3)
1. the quick after-ripening of pasque flower seed and germination method is characterized in that step is as follows:
(1) the pasque flower seed is with 2.10～2.30 mgL
-1KT, 2.90～3.20 mgL
-1GA
3With 0.30～0.60 mgL
-12, after the 4-D mixed solution soaks, seed and river sand are mixed in the trogue of all packing into afterwards, place the indoor alternating temperature processing of carrying out 24～28 d;
(2) alternating temperature is processed amplitude and time: day temperature is 25 ± 2 ℃, processes 14 h, and nocturnal temperature is 10 ± 2 ℃, processes 10 h; Can directly sow after processing 24～28 d.
2. according to the quick after-ripening of pasque flower seed claimed in claim 1 and germination method, it is characterized in that seed soak time 18 h.
3. according to claim 1 or the 2 quick after-ripening of described pasque flower seed and germination methods, it is characterized in that seed: river sand=1:2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310018967.8A CN103039157B (en) | 2013-01-18 | 2013-01-18 | Rapid after-ripening and germination method of pulsatilla koreana seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310018967.8A CN103039157B (en) | 2013-01-18 | 2013-01-18 | Rapid after-ripening and germination method of pulsatilla koreana seeds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103039157A true CN103039157A (en) | 2013-04-17 |
| CN103039157B CN103039157B (en) | 2014-07-09 |
Family
ID=48052209
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310018967.8A Active CN103039157B (en) | 2013-01-18 | 2013-01-18 | Rapid after-ripening and germination method of pulsatilla koreana seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103039157B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365211A (en) * | 2014-09-12 | 2015-02-25 | 陈小洁 | Method for breaking pulsatilla chinensis (bunge) regel seed dormancy |
| CN106818178A (en) * | 2017-02-09 | 2017-06-13 | 合肥隆扬农业科技有限公司 | The implantation methods of the Chinese bulbul |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006094400A1 (en) * | 2005-03-10 | 2006-09-14 | Cellfor Inc. | Aerated liquid priming of conifer somatic germinants |
| CN101702981A (en) * | 2009-11-30 | 2010-05-12 | 时维静 | Method for artificially culturing Chinese medicinal plant of Chinese pulsatilla root |
| CN102668765A (en) * | 2012-04-28 | 2012-09-19 | 湖南农业大学 | Method for raising seedlings of redrlowered loropetalum |
-
2013
- 2013-01-18 CN CN201310018967.8A patent/CN103039157B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006094400A1 (en) * | 2005-03-10 | 2006-09-14 | Cellfor Inc. | Aerated liquid priming of conifer somatic germinants |
| CN101702981A (en) * | 2009-11-30 | 2010-05-12 | 时维静 | Method for artificially culturing Chinese medicinal plant of Chinese pulsatilla root |
| CN102668765A (en) * | 2012-04-28 | 2012-09-19 | 湖南农业大学 | Method for raising seedlings of redrlowered loropetalum |
Non-Patent Citations (3)
| Title |
|---|
| 时维静 等: "白头翁种子发芽特性研究", 《种子》 * |
| 李海燕 等: "不同处理对朝鲜白头翁种子萌发的影响", 《西北农业学报》 * |
| 许桂芳 等: "毛莨科三种野生观赏植物种子发芽条件的研究", 《种子》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365211A (en) * | 2014-09-12 | 2015-02-25 | 陈小洁 | Method for breaking pulsatilla chinensis (bunge) regel seed dormancy |
| CN106818178A (en) * | 2017-02-09 | 2017-06-13 | 合肥隆扬农业科技有限公司 | The implantation methods of the Chinese bulbul |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103039157B (en) | 2014-07-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102870564B (en) | Fast efficient propagation method of Paris polyphylla | |
| CN102577803B (en) | Method for rapidly culturing improved mulberry seedlings by using sheared waste mulberry branches | |
| CN103733930A (en) | Drip irrigation under plastic film method for rice cropping | |
| CN105230333A (en) | Rotation planting technology for saffron crocus with rice, peony and green vegetables | |
| CN104067939A (en) | Tissue culture rapid propagation method of radix gentianae | |
| CN104541881A (en) | Planting method of rose | |
| CN105850450A (en) | Soybean planting method | |
| CN107027372A (en) | A kind of method for promoting red gentian seed to sprout and its seedling | |
| CN104054502B (en) | Ecological pepper cultivating method | |
| CN103109747B (en) | Rapid pseudolarix propagation method based on stem node propagation | |
| CN103039240B (en) | Seeding and seedling raising method for waterbirch in Three Gorges Reservoir region | |
| CN107306632A (en) | A kind of culturing and reproducing of dahlia | |
| CN104429576A (en) | Liquorice planting method | |
| CN104126398B (en) | The pine forest interplanting Chinese medicine bletilla striata is combined breeding method | |
| CN102960095B (en) | Rapid after-ripening method of stichopus japonicus seed | |
| CN103039157B (en) | Rapid after-ripening and germination method of pulsatilla koreana seeds | |
| CN113213996A (en) | Root promoting agent for promoting growth of cigar root system and preparation and use methods thereof | |
| CN101743908A (en) | Tissue culture, rapid propagation and cultivation method of grevillea banksii | |
| CN107241996A (en) | A kind of cultural method of the peaceful root of purple-flowered peucedanum | |
| CN104322267A (en) | Interplanting method of shiny-leaved yellowhorn and sedum sarmentosum | |
| CN104170616A (en) | Artificial cultivation method of radix clematidis | |
| CN107155579A (en) | A kind of seed seedling-raising method of Flos micheliae Albae | |
| CN106105619A (en) | A kind of Herba Spinaciae implantation methods | |
| CN106431656A (en) | Sowing and seedling culture method of pittosporum tobira | |
| CN109892177A (en) | A kind of cold area's Trollius ledebouri greenhouse direct-seeding planting method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |