CN102919522A - Preparation method for feed for ruminants - Google Patents
Preparation method for feed for ruminants Download PDFInfo
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- CN102919522A CN102919522A CN2012104325821A CN201210432582A CN102919522A CN 102919522 A CN102919522 A CN 102919522A CN 2012104325821 A CN2012104325821 A CN 2012104325821A CN 201210432582 A CN201210432582 A CN 201210432582A CN 102919522 A CN102919522 A CN 102919522A
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Abstract
The invention relates to a preparation method for feed, in particular to a preparation method for feed for ruminants. The method includes: adding water and dissolving corn steep liquor, urea, ammonium sulfate, monopotassium phosphate and magnesium sulfate, mixing evenly, stirring and mixing with crop straw powder, wheat bran and bean cake powder, mixing evenly, sterilizing and cooling to 28 DEG C; and inoculating needle mushroom seed liquid and candida utilis seed liquid into a solid medium respectively, fermenting at constant temperature of 28 DEG C, and drying at low temperature to obtain the product. During a fermenting process, a large amount of microbial thallus breed, and abundant vitamin, microbial active enzymes and unknown growth factors are generated. After the ruminants eat the feed, powerful beneficial microbial population can be formed in intestinal canals, pathogenic bacterium groups are restrained, effects which common antibiotic can not achieve are achieved, immunity of animals is improved, conversion rate of the feed is greatly improved, growth of the animals is promoted, and effective output rate of the animals is improved.
Description
Technical field
The present invention relates to the preparation method of a kind of preparation method of feed, particularly a kind of ruminant feed.
Background technology
The agricultural crop straws such as corn stalk, kaoliang stalk, straw, peanut vine, potato seedling, beanstalk are owing to containing a large amount of celluloses, the ruminants such as ox, sheep roughages commonly used, these feeds are just fed through being directly used in after simple the pulverizing usually, so not only the utilization rate of active components in the feed is low, and the edible such feed of Long term Animal can cause malnutrition, hypoimmunity has increased the probability that animal falls ill, and has reduced the meat of animal, the output of milk.
Summary of the invention
The object of the invention is to provides a kind of nutrient composition content that can increase in the ruminant feed for above-mentioned deficiency, improves efficiency of feed utilization, improves ruminant immunity, increases the preparation method of the ruminant feed of ruminant throughput rate.
The object of the present invention is achieved like this:
1, the preparation of seed liquor
1. the preparation of Asparagus seed liquor
Asparagus seed liquor culture medium prescription is: 3% soluble starch, 2% beancake powder, 1% corn flour, 0.2% yeast extract, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate.Get the 100ml seed liquor and cultivate based in the 500ml triangular flask, 121 ℃ of sterilizations 20 minutes are cooled to 28 ℃, and inoculation Asparagus inclined-plane seed was cultivated 6 days under rotary shaker 150rpm, 26～28 ℃ condition, namely got the Asparagus seed liquor.
2. the preparation of candida utili seed liquor
Take by weighing corn flour 100 grams, place triangular flask, add water 1000ml, shake up, ratio in 10 units of every gram corn flour adds thermostable α-amylase, and insulation is 30 minutes under 90～95 ℃ of conditions, is cooled to 60 ℃, ratio in 100 units of every gram corn flour adds carbohydrase, keeps 58 ℃～60 ℃ saccharification 5 hours.Get 100 milliliters of saccharified liquids, add 0.3 gram urea, 0.1 gram potassium dihydrogen phosphate, 0.05 gram magnesium sulfate, sterilized 20 minutes for 121 ℃, be cooled to 28 ℃, from inclined-plane inoculation one ring candida utili, under 28 ℃, the condition of rotary shaker 150rpm, cultivated 16～18 hours, namely get the candida utili seed liquor.
2, the making of ruminant feed
With the agricultural crop straw powder, wheat bran, beancake powder mixes in the ratio of 85:10:5, with the agricultural crop straw powder, wheat bran, the corn steep liquor of beancake powder gross weight 5%, 1.5% urea, 1.5% ammonium sulfate, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate is dissolved in water and admixes the agricultural crop straw powder behind the mixing, wheat bran, in the beancake powder, moisture content in medium is controlled at 60～65%, mix, sterilized 20 minutes for 121 ℃, be cooled to 28 ℃, the Asparagus seed liquor is inoculated in the above-mentioned solid medium, inoculum concentration is 8%, mix rear tiling to the bamboo curtain splint of sterilization, 4～6 centimetres of thickness, fermentation is 72 hours in 28 ℃ of insulating boxs, and then the candida utili seed liquor is inoculated in the above-mentioned solid medium, inoculum concentration is 8%, mixes, and continues 28 ℃ of ferment at constant temperature 4 days, under 45 ℃ of conditions, be dried to afterwards moisturely below 10%, get product.
Ruminant feed of the present invention, during the fermentation, a large amount of microbial cells breedings produce abundant vitamin, microbial activity enzyme and UGF.After ruminant is edible, in enteron aisle, can form strong beneficial microbe population advantage, thereby inhibition pathogenic flora, played the effect of being beyond one's reach of general antibiotic, strengthened the immunity of animal, greatly improve the conversion ratio of feed, promoted the growth of animal, improved the effective output capacity of animal.
(4), specific embodiments
2 bacterial strains that the present invention adopts are purchased in Chinese industrial microorganism fungus kind preservation administrative center, are respectively: Asparagus CICC50051, candida utili CICC31395.
Mode one:
1, the preparation of seed liquor
1. the preparation of Asparagus seed liquor
The corn flour, 0.2 that takes by weighing 3 gram soluble starches, 2 gram beancake powders, 1 gram restrains the potassium dihydrogen phosphate of yeast extract, 0.1 gram, the magnesium sulfate of 0.05 gram, add water and be settled to 100 milliliters, pour in the 500ml triangular flask, sterilized 20 minutes for 121 ℃, be cooled to 28 ℃, inoculation Asparagus inclined-plane seed was cultivated 6 days under rotary shaker 150rpm, 26～28 ℃ condition, namely got the Asparagus seed liquor.
2. the preparation of candida utili seed liquor
Take by weighing corn flour 100 grams, place triangular flask, add water 1000ml, shake up, add thermostable α-amylase in the ratio of 10 units of every gram corn flour, insulation is 30 minutes under 95 ℃ of conditions, be cooled to 60 ℃, in the ratio adding carbohydrase of 100 units of every gram corn flour, keep 60 ℃ of saccharification 5 hours.Get 100 milliliters of saccharified liquids, add 0.3 gram urea, 0.1 gram potassium dihydrogen phosphate, 0.05 gram magnesium sulfate, sterilized 20 minutes for 121 ℃, be cooled to 28 ℃, from inclined-plane inoculation one ring candida utili, under 28 ℃, the condition of rotary shaker 150rpm, cultivated 18 hours, namely get the candida utili seed liquor.
2, the making of ruminant feed
Take by weighing corn stalk powder 850 grams, wheat bran 100 grams, beancake powder 50 grams mix, with 50 gram corn steep liquors, the urea of 15 grams, the ammonium sulfate of 15 grams, the potassium dihydrogen phosphate of 1 gram, 0.5 being dissolved in water, the magnesium sulfate of gram admixes corn stalk powder behind the mixing, wheat bran, in the beancake powder, moisture content in medium is controlled at 65%, mix, sterilized 20 minutes for 121 ℃, be cooled to 28 ℃, 80 milliliters of Asparagus seed liquor are inoculated in the above-mentioned solid medium, mix rear tiling to the bamboo curtain splint of sterilization, 5 centimetres of thickness, fermentation is 72 hours in 28 ℃ of insulating boxs, and then 80 milliliters of candida utili seed liquor are inoculated in the above-mentioned solid medium, mix, continued 28 ℃ of ferment at constant temperature 4 days, under 45 ℃ of conditions, be dried to afterwards moisturely below 10%, get product.
Mode two:
1, the preparation of seed liquor
1. the preparation of Asparagus seed liquor
The corn flour, 0.2 that takes by weighing 3 gram soluble starches, 2 gram beancake powders, 1 gram restrains the potassium dihydrogen phosphate of yeast extract, 0.1 gram, the magnesium sulfate of 0.05 gram, add water and be settled to 100 milliliters, pour in the 500ml triangular flask, sterilized 20 minutes for 121 ℃, be cooled to 28 ℃, inoculation Asparagus inclined-plane seed was cultivated 6 days under rotary shaker 150rpm, 26～28 ℃ condition, namely got the Asparagus seed liquor.
2. the preparation of candida utili seed liquor
Take by weighing corn flour 100 grams, place triangular flask, add water 1000ml, shake up, add thermostable α-amylase in the ratio of 10 units of every gram corn flour, insulation is 30 minutes under 90 ℃ of conditions, be cooled to 60 ℃, in the ratio adding carbohydrase of 100 units of every gram corn flour, keep 60 ℃ of saccharification 5 hours.Get 100 milliliters of saccharified liquids, add 0.3 gram urea, 0.1 gram potassium dihydrogen phosphate, 0.05 gram magnesium sulfate, sterilized 20 minutes for 121 ℃, be cooled to 28 ℃, from inclined-plane inoculation one ring candida utili, under 28 ℃, the condition of rotary shaker 150rpm, cultivated 18 hours, namely get the candida utili seed liquor.
2, the making of ruminant feed
Take by weighing rice straw powder 850 grams, wheat bran 100 grams, beancake powder 50 grams mix, with 50 gram corn steep liquors, the urea of 15 grams, the ammonium sulfate of 15 grams, the potassium dihydrogen phosphate of 1 gram, 0.5 being dissolved in water, the magnesium sulfate of gram admixes rice straw powder behind the mixing, wheat bran, in the beancake powder, moisture content in medium is controlled at 65%, mix, sterilized 20 minutes for 121 ℃, be cooled to 28 ℃, 80 milliliters of Asparagus seed liquor are inoculated in the above-mentioned solid medium, mix rear tiling to the bamboo curtain splint of sterilization, 4 centimetres of thickness, fermentation is 72 hours in 28 ℃ of insulating boxs, and then 80 milliliters of candida utili seed liquor are inoculated in the above-mentioned solid medium, mix, continued 28 ℃ of ferment at constant temperature 4 days, under 45 ℃ of conditions, be dried to afterwards moisturely below 10%, get product.
Claims (1)
1. the preparation method of a ruminant feed, it is characterized in that: with the agricultural crop straw powder, wheat bran, beancake powder mixes in the ratio of 85:10:5, with the agricultural crop straw powder, wheat bran, the corn steep liquor of beancake powder gross weight 5%, 1.5% urea, 1.5% ammonium sulfate, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate is dissolved in water and admixes the agricultural crop straw powder behind the mixing, wheat bran, in the beancake powder, moisture content in medium is controlled at 60～65%, mix, sterilized 20 minutes for 121 ℃, be cooled to 28 ℃, the Asparagus seed liquor is inoculated in the above-mentioned solid medium, inoculum concentration is 8%, mix rear tiling to the bamboo curtain splint of sterilization, 4～6 centimetres of thickness, fermentation is 72 hours in 28 ℃ of insulating boxs, and then the candida utili seed liquor is inoculated in the above-mentioned solid medium, and inoculum concentration is 8%, mix, continued 28 ℃ of ferment at constant temperature 4 days, and under 45 ℃ of conditions, be dried to afterwards moisturely below 10%, get product.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283945A (en) * | 2013-06-08 | 2013-09-11 | 金华市金帆饲料有限公司 | Method for preparing complex enzyme-probiotic preparation for feeds through biotransformation of bagasse and sunflower cakes |
| CN107853455A (en) * | 2017-12-06 | 2018-03-30 | 邹家勇 | Organic feed for ruminant and preparation method thereof |
| CN109430549A (en) * | 2018-12-14 | 2019-03-08 | 马山县山山农业科技有限公司 | The method for preparing feed using the digestion of edible mushroom |
Citations (4)
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| CN1139520A (en) * | 1996-03-05 | 1997-01-08 | 高银相 | Production method for biological multi-bacterial fermented fodder made from stalks |
| CN1552233A (en) * | 2003-05-26 | 2004-12-08 | 哈尔滨中冠生物工程有限公司 | Method for producing multi-vitamins and multi-enzymes high protein microorganic agent by multi-bacteria solid fermentation |
| CN101023775A (en) * | 2006-02-20 | 2007-08-29 | 郑爱国 | Microbe feed additive |
| CN101606579A (en) * | 2009-07-03 | 2009-12-23 | 农业部环境保护科研监测所 | Straw feed of a kind of fermented by white rot fungus and preparation method thereof |
-
2012
- 2012-11-02 CN CN2012104325821A patent/CN102919522A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1139520A (en) * | 1996-03-05 | 1997-01-08 | 高银相 | Production method for biological multi-bacterial fermented fodder made from stalks |
| CN1552233A (en) * | 2003-05-26 | 2004-12-08 | 哈尔滨中冠生物工程有限公司 | Method for producing multi-vitamins and multi-enzymes high protein microorganic agent by multi-bacteria solid fermentation |
| CN101023775A (en) * | 2006-02-20 | 2007-08-29 | 郑爱国 | Microbe feed additive |
| CN101606579A (en) * | 2009-07-03 | 2009-12-23 | 农业部环境保护科研监测所 | Straw feed of a kind of fermented by white rot fungus and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 吴萍 等: "发酵废菌渣生产饲料蛋白的研究", 《食品工业科技》, vol. 31, no. 8, 1 August 2010 (2010-08-01) * |
| 李学梅: "食用菌菌渣的开发利用", 《河南农业科学》, no. 5, 15 May 2003 (2003-05-15), pages 40 - 42 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283945A (en) * | 2013-06-08 | 2013-09-11 | 金华市金帆饲料有限公司 | Method for preparing complex enzyme-probiotic preparation for feeds through biotransformation of bagasse and sunflower cakes |
| CN107853455A (en) * | 2017-12-06 | 2018-03-30 | 邹家勇 | Organic feed for ruminant and preparation method thereof |
| CN109430549A (en) * | 2018-12-14 | 2019-03-08 | 马山县山山农业科技有限公司 | The method for preparing feed using the digestion of edible mushroom |
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Application publication date: 20130213 |