CN102870524A - Method for breaking hard seed dormancy in astragalus seeds - Google Patents
Method for breaking hard seed dormancy in astragalus seeds Download PDFInfo
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- CN102870524A CN102870524A CN 201210339009 CN201210339009A CN102870524A CN 102870524 A CN102870524 A CN 102870524A CN 201210339009 CN201210339009 CN 201210339009 CN 201210339009 A CN201210339009 A CN 201210339009A CN 102870524 A CN102870524 A CN 102870524A
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Abstract
The invention discloses a method for breaking hard seed dormancy in astragalus seeds, relates to a method for producing the astragalus seeds, and belongs to the field of traditional Chinese medicine planting. The method is characterized by comprising the following steps of: soaking the astragalus seeds into 70 to 75 percent alcohol for 5 to 10 minutes for sterilization, and washing the astragalus seeds by using sterilized water for 2 to 3 times; and after guar gum culture medium is fully grown with microorganisms, spreading the astragalus seeds on the culture medium in a density of 26 to 34 seeds/cm<2> at the temperature of between 25 and 30 DEG C for 24 to 96 hours. The method has the advantages that the astragalus seeds are treated by one of trichoderma harzianum, aspergillus oryzae and aspergillus niger or the mixture of two or all of trichoderma harzianum, aspergillus oryzae and aspergillus niger, the hard seed dormancy in the astragalus seeds can be effectively broken, and the germination rate is improved by 10 to 30 percent; and the trichoderma harzianum, aspergillus oryzae and aspergillus niger or the mixture thereof are or is easily obtained and cultured, and can be used for preparing a coating agent or a seed dressing microbial agent for the astragalus seeds, cost is low, and the method has a quite wide market prospect in the production practice of astragalus seeds.
Description
Technical field
The present invention relates to the astragalus membranaceus seed production method, belong to the Chinese medicine field of planting, specifically a kind of method of breaking the strong dormancy of astragalus membranaceus seed.
Background technology
The Radix Astragali has another name called yellow over sixty years of age, is the general designation of plant and traditional Chinese medicine.The plant Radix Astragali originates in the ground such as the Inner Mongol, Shanxi, Gansu, Heilungkiang, is state three level protecting plant.Modern study, the Radix Astragali contain the various trace elements such as saponin, sucrose, polysaccharide, several amino acids, folic acid and selenium, zinc, copper.The enhanced machine body immunity function arranged, protect the liver, diuresis, anti-ageing, anti-stress, step-down and antibacterial action more widely.Wild Radix Astragali output is seldom supplied market demand by artificial planting in recent years.The nice and cool weather of Radix Astragali happiness, drought-enduring cold-resistant, be afraid of waterlogging, deep with soil layer, be rich in humus, neutral sandy loam that permeable power is strong is advisable.Astragalus membranaceus seed has strong phenomenon, i.e. losing property of seed epidermis even can not sprout under suitable external environmental condition in imbibition.
Through the literature search of prior art is found have sunlight to be exposed to the sun to breaking the strong common method of Papilionaceae grass seed, hot-water soak, physics are milled, chemicals treatment (concentrated sulfuric acid or pak-chor) etc.
But, adopt above method to break strong hard crust of legume seeds dormancy, seed for not of the same race, kind, differing maturity and the different time limits of preservation, the differences such as processing time (sunlight is exposed to the sun), treatment temperature and concentration (hot-water soak, chemicals treatment) and processing pressure (physics is milled) are all very large, be difficult for controlling, chemical method also has harmfulness to seed simultaneously; Treatment effect is often unsatisfactory in the production practices; Because astragalus membranaceus seed is less, for the strong dormancy of breaking astragalus membranaceus seed, also there is not desirable solution at present.
Summary of the invention
For deficiency and the defective of breaking the strong dormancy method existence of astragalus membranaceus seed in the above-mentioned background technology, the object of the invention is to, a kind of method of breaking the strong dormancy of astragalus membranaceus seed is provided, the method adopts the combination bacterial classification to process astragalus membranaceus seed, thoroughly solve the key of breaking the strong dormancy method of astragalus membranaceus seed, can improve germination rate and the emergence rate of its seed; Employed microorganism mould, can be made into astragalus membranaceus seed seed coating medicine or seed dressing microbial inoculum, be directly used in the production practices, reduced some before the sowing and broken the strong link of seed, saved manpower and materials, and production cost has been low, benefit is large, easy to use, alleviated in recent years wild Radix Astragali output seldom, by artificial planting supply market demand.
For achieving the above object, a kind of method of breaking the strong dormancy of astragalus membranaceus seed of the present invention is characterized in that, comprises the following steps:
One, gets raw material preparation medium by following weight ratio: guar gum: peptone: MgS047H20: KH2P04: agar: distilled water=30: 2: 1: 2: 30: 2000; Each composition is dissolved in the distilled water successively, and the limit edged shakes up, and 121 ℃ of sterilization 15min are after the medium condensation, stand-by;
Two, inoculate mould at above-mentioned guar gum medium, described mould is that trichoderma harzianum wood is mould, aspergillus oryzae, aspergillus niger one of them, the perhaps in twos mixing of above-mentioned bacterial classification, perhaps the three of above-mentioned bacterial classification mixes; Under 25～30 ℃ of conditions, cultivated 2～7 days, take fungus growth fully as the degree;
Three, process astragalus membranaceus seed:
1. astragalus membranaceus seed is sterilized with 70～75% alcohol seed soaking, 5～10min, with sterile water washing 2～3 times;
2. when treating to cover with microorganism on the medium, be sprinkled into therein astragalus membranaceus seed, density keeps 24～96h to get final product with 26～34/cm2 under 25～30 ℃ of conditions.
Described guar gum has the function of suspension, emulsification, and outstanding dispersibility is arranged in cold water and the hot water, can not produce clustering phenomena, has improved the convenience of manufacturing and production operation; Agar is that fixative has coagulability, stability, as coagulating agent liquid nutrient medium is converted into solid culture medium or semisolid culturemedium by adding agar.
Described peptone, MgSO47H2O, KH2PO4: the nutriments such as C source, N source, growth factor can be provided for microorganism.
A kind of method of breaking the strong dormancy of astragalus membranaceus seed of the present invention, its beneficial effect is: adopt mould: trichoderma harzianum wood is mould, aspergillus oryzae, aspergillus niger any single culture wherein, or mix in twos among the three or bacterial classification that the three mixes is processed astragalus membranaceus seed, can both effectively break the strong dormancy of astragalus membranaceus seed, improve germination rate 10～30%; The bacterial classification of above-mentioned 3 moulds or its Mixed Microbes are easy to obtain and cultivate, and with low cost, make astragalus membranaceus seed seed coating medicine or seed dressing microbial inoculum, have than larger market prospect in the astragalus membranaceus seed production practices.
Embodiment
Following examples are that the present invention further goes out to set forth.
Embodiment 1
1. get raw material preparation medium by following weight ratio: guar gum: peptone: MgSO47H2O: KH2PO4: agar: distilled water=30: 2: 1: 2: 30: 2000; Each composition is dissolved in the distilled water successively, and the limit edged shakes up, and 121 ℃ of sterilization 15min are after the medium condensation, stand-by;
2. inoculate mould at above-mentioned guar gum medium, described mould is that trichoderma harzianum wood is mould, aspergillus oryzae, aspergillus niger one of them, the perhaps in twos mixing of above-mentioned bacterial classification, perhaps the three of above-mentioned bacterial classification mixes; Under 25 ℃ of conditions, cultivated 2 days, take fungus growth fully for spending;
3. processing astragalus membranaceus seed:
1. astragalus membranaceus seed is sterilized with 70% alcohol seed soaking 5min, with sterile water washing 2 times;
When 2. treating to cover with microorganism on the medium, be sprinkled into therein astragalus membranaceus seed, density keeps 24h to get final product under 25 ℃ of conditions with 26/cm2.
Table 1 adopts the mould processing to break the germination result of the strong dormancy of astragalus membranaceus seed
As seen from Table 1, adopt mould to process and break the strong dormancy of astragalus membranaceus seed, standard germination percentage reaches 93%, and contrast group (not taking over what bacterial classification) improves 20%.
Embodiment 2
1. get raw material preparation medium by following weight ratio: guar gum: peptone: MgSO47H2O: KH2PO4: agar: distilled water=30: 2: 1: 2: 30: 2000; Each composition is dissolved in the distilled water successively, and the limit edged shakes up, and 121 ℃ of sterilization 15min are after the medium condensation, stand-by;
2. inoculate mould at above-mentioned guar gum medium, described mould is that trichoderma harzianum wood is mould, aspergillus oryzae, aspergillus niger one of them, the perhaps in twos mixing of above-mentioned bacterial classification, perhaps the three of above-mentioned bacterial classification mixes; Under 30 ℃ of conditions, cultivated 7 days, take fungus growth fully for spending;
3. processing astragalus membranaceus seed:
1. astragalus membranaceus seed is sterilized with 75% alcohol seed soaking 10min, with sterile water washing 3 times;
When 2. treating to cover with microorganism on the medium, be sprinkled into therein astragalus membranaceus seed, density keeps 96h to get final product under 30 ℃ of conditions with 34/cm2.
Table 2 adopts the mould processing to break the germination result of the strong dormancy of astragalus membranaceus seed
As seen from Table 2, adopt mould to process and break the strong dormancy of astragalus membranaceus seed, standard germination percentage reaches 95%, and contrast group (not taking over what bacterial classification) improves 20%.
Claims (3)
1. a method of breaking the strong dormancy of astragalus membranaceus seed is characterized in that: astragalus membranaceus seed with 70～75% alcohol seed soaking, 5～10min sterilization, is washed 2～3 times with sterile water; When treating to cover with microorganism on the guar gum medium, be sprinkled into therein astragalus membranaceus seed, density keeps 24～96h to get final product with 26～34/cm2 under 25～30 ℃ of conditions.
2. a kind of method of breaking the strong dormancy of astragalus membranaceus seed as claimed in claim 1, it is characterized in that: the compound method of guar gum medium is: get the raw material preparation by following weight ratio: guar gum: peptone: MgSO47H2O: KH2PO4: agar: distilled water=30: 2: 1: 2: 30: 2000; Each composition is dissolved in the distilled water successively, and the limit edged shakes up, 121 ℃ of sterilization 15min.
3. a kind of method of breaking the strong dormancy of astragalus membranaceus seed as claimed in claim 1, it is characterized in that: inoculate mould on the guar gum medium, described mould is that trichoderma harzianum wood is mould, aspergillus oryzae, aspergillus niger one of them, the perhaps in twos mixing of above-mentioned bacterial classification, perhaps the three of above-mentioned bacterial classification mixes; Under 25～30 ℃ of conditions, cultivated 2～7 days.
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| CN 201210339009 CN102870524A (en) | 2012-09-13 | 2012-09-13 | Method for breaking hard seed dormancy in astragalus seeds |
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| CN 201210339009 CN102870524A (en) | 2012-09-13 | 2012-09-13 | Method for breaking hard seed dormancy in astragalus seeds |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638020A (en) * | 2014-11-10 | 2016-06-08 | 王玉红 | Treatment method for milkvetch root seeds |
| CN105900558A (en) * | 2016-04-18 | 2016-08-31 | 甘肃赫博陇药科技有限责任公司 | Pests and diseases prevention and rapid synchronous germination processing method of astragalus membranaceus seeds |
| CN106105488A (en) * | 2016-06-23 | 2016-11-16 | 青岛蔚蓝生物集团有限公司 | A kind of method improving Quality of Seedlings |
| CN108738484A (en) * | 2018-06-25 | 2018-11-06 | 安徽农业大学 | A kind of Kiwi berry seed initiator and cause method |
| CN113179704A (en) * | 2021-06-18 | 2021-07-30 | 东北农业大学 | Method for improving germination rate of Chinese medicinal material astragalus seeds |
-
2012
- 2012-09-13 CN CN 201210339009 patent/CN102870524A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638020A (en) * | 2014-11-10 | 2016-06-08 | 王玉红 | Treatment method for milkvetch root seeds |
| CN105638020B (en) * | 2014-11-10 | 2018-02-16 | 西和县志强中药材开发有限公司 | A kind of processing method of astragalus membranaceus seed |
| CN105900558A (en) * | 2016-04-18 | 2016-08-31 | 甘肃赫博陇药科技有限责任公司 | Pests and diseases prevention and rapid synchronous germination processing method of astragalus membranaceus seeds |
| CN105900558B (en) * | 2016-04-18 | 2018-06-15 | 甘肃赫博陇药科技有限责任公司 | A kind of astragalus membranaceus seed no disease and pests harm and Fast synchronization germination treatment method of wax |
| CN106105488A (en) * | 2016-06-23 | 2016-11-16 | 青岛蔚蓝生物集团有限公司 | A kind of method improving Quality of Seedlings |
| CN106105488B (en) * | 2016-06-23 | 2019-06-07 | 青岛蔚蓝生物集团有限公司 | A method of improving Quality of Seedlings |
| CN108738484A (en) * | 2018-06-25 | 2018-11-06 | 安徽农业大学 | A kind of Kiwi berry seed initiator and cause method |
| CN108738484B (en) * | 2018-06-25 | 2021-05-25 | 安徽农业大学 | Kiwi seed initiator and initiation method |
| CN113179704A (en) * | 2021-06-18 | 2021-07-30 | 东北农业大学 | Method for improving germination rate of Chinese medicinal material astragalus seeds |
| CN113179704B (en) * | 2021-06-18 | 2024-04-26 | 东北农业大学 | Method for improving germination rate of astragalus seeds of traditional Chinese medicinal materials |
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Application publication date: 20130116 |