CN102838673B - 修饰抗体的方法和具有改善的功能性质的修饰抗体 - Google Patents
修饰抗体的方法和具有改善的功能性质的修饰抗体 Download PDFInfo
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Classifications
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/241—Tumor Necrosis Factors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/567—Framework region [FR]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
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- Wood Science & Technology (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- Plant Pathology (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
Description
残基 | VH4 | VH 12 | VH 103 | VH 144 | VL 15 | VL 147 |
A | 0.23046215 | 0 | 0 | 0 | 3.8647343 | 0.176821923 |
C | 0 | 0 | 0 | 0 | 0 | 0 |
D | 0 | 0 | 0 | 0 | 0 | 0 |
E | 0 | 0 | 0 | 0 | 0 | 0 |
F | 0.483091787 | 0 | 0.483091787 | 0 | 0 | 0 |
G | 0 | 0 | 0 | 0 | 0 | 0 |
H | 0 | 0 | 0 | 0 | 0 | 0 |
I | 0 | 2.415458937 | 9.661835749 | 0 | 5.314009662 | 70.38834951 |
K | 0 | 0 | 0 | 0 | 0 | 0 |
L | 96.61835749 | 89.85507246 | 7.246376812 | 27.0531401 | 45.89371981 | 15.53398058 |
M | 0 | 0 | 10.62801932 | 1.93236715 | 0 | 0.970873786 |
N | 0 | 0 | 0 | 0 | 0 | 0 |
P | 0.966183575 | 0 | 0 | 0.966183575 | 21.73913043 | 0.485436893 |
Q | 0 | 0 | 0 | 0.483091787 | 0 | 0 |
R | 0 | 0 | 7.246376812 | 0 | 0 | 0 |
S | 0 | 0.966183575 | 0 | 18.84057971 | 0 | 0 |
T | 0 | 0 | 15.4589372 | 50.72463768 | 0.966183575 | 0 |
V | 1.93236715 | 6.763285024 | 49.27536232 | 0 | 22.22222222 | 12.62135922 |
W | 0 | 0 | 0 | 0 | 0 | 0 |
Y | 0 | 0 | 0 | 0 | 0 | 0 |
蛋白质 | 蛋白质丧失(不溶性聚集物) |
ESBA105 | 1.14% |
ESBA105 Opt 1_0 | 8.17% |
ESBA105 Opt 0_2 | 4.45% |
ESBA105 Opt 1_2 | 46.60% |
ESBA105 VH V103T | -1.95% |
Claims (1)
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CN200880021889.4A CN101849001B (zh) | 2007-06-25 | 2008-06-25 | 修饰抗体的方法和具有改善的功能性质的修饰抗体 |
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CN102838673A CN102838673A (zh) | 2012-12-26 |
CN102838673B true CN102838673B (zh) | 2016-05-11 |
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CN201510357753.2A Pending CN105017427A (zh) | 2007-06-25 | 2008-06-25 | 修饰抗体的方法和具有改善的功能性质的修饰抗体 |
CN201310383003.3A Active CN103435696B (zh) | 2007-06-25 | 2008-06-25 | 修饰抗体的方法和具有改善的功能性质的修饰抗体 |
CN201610852278.0A Active CN106366192B (zh) | 2007-06-25 | 2008-06-25 | 修饰抗体的方法和具有改善的功能性质的修饰抗体 |
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CN200880021889.4A Active CN101849001B (zh) | 2007-06-25 | 2008-06-25 | 修饰抗体的方法和具有改善的功能性质的修饰抗体 |
CN201510357753.2A Pending CN105017427A (zh) | 2007-06-25 | 2008-06-25 | 修饰抗体的方法和具有改善的功能性质的修饰抗体 |
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